| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 574,998 | C→A | 8.4% | A12D (GCC→GAC) | fdoG → | formate dehydrogenase‑O major subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 574,998 | 0 | C | A | 8.4% | 180.9 / 4.8 | 58 | A12D (GCC→GAC) | fdoG | formate dehydrogenase‑O major subunit |
| Reads supporting (aligned to +/- strand): ref base C (26/27); new base A (2/3); total (28/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.75e-01 | |||||||||||
ATTCTTGACTAGTCTTAACCTTTGGCGGCCATCAATTGGCCGCCACCGTGACTACCAAGAGGCGCCGGCAATGTTCCGCGCGTCCACCACCTGATCAGGAGTGCACGATGGACCTGAACC‑‑GTCGGCAGTTCTTCAAGGTCGCCGCCGTCGGCCTTGGAGGCTCGAGCCTTGCGGCGTTGGGCATGGCCCCAACGCCGGCATTCGCCGAGCAGGTGCGTCACTTCAAGCTGGCGCATACCAAAGAAACCCGTAACACCTGCCCCTACTGTTCGGTCGGCTGCGGCCTGATC > NC_002947/574857‑575146 | aTTCTTGACTAGTCTTAACCTTTGGCGGCCATCAATTGGCCGCCACCGTGACTACCAAGAGGCGCCGGCAATGTTCCGCGCGTCCACCACCTGATCAGGAGTGCACGATGGACCTGAACC‑‑GTCGGCAGTTCTTCAAGGTCGCCGCCGTc < 3:123396/149‑1 (MQ=255) ttCTTGACTAGTCTTAACCTTTGGCGGCCATCAATTGGCCGCCACCGTGACTACCAAGAGGCGCCGGCAATGTTCCGCGCGTCCACCACCTGATCAGGAGTGCACGATGGACCTGAACC‑‑GTCGGCAGTTCTTCAAGGTCGCCGCCGTCg < 5:91661/149‑1 (MQ=255) gACTAGTCTTAACCTTTGGCGGCCATCAATTGGCCGCCACCGTGACTACCAAGAGGCGCCGGCAATGTTCCGCGCGTCCACCACCTGATCAGGAGTGCACGATGGACCTGAACC‑‑GTCGGCAGTTCTTCAAGGTCGCCGCCGTCGGCCt < 2:230565/148‑1 (MQ=255) aCTAGTCTTAACCTTTGGCGGCCATCAATTGGCCGCCACCGTGACTACCAACAGGCGCCGGCAATGTTCCGCGCGTCCACCACCTGATCAGGAGTGCACGATGGACCTGAACC‑‑GTCGGCAGTTCTTCAAGGTCGCCGCCGTCGGCCtt < 2:48857/148‑1 (MQ=255) gTCTTAACCTTTGGCGGCCATCAATTGGCCGCCACCGTGACTACCAAGAGGCGCCGGCAATGTTCCGCGCGTCCACCACCTGATCAGGAGTGCACGATGGACCTGAACC‑‑GTCGGCAGTTCTTCAAGGTCGCCGCCGTCGGCCTTGGAgg > 5:114595/1‑149 (MQ=255) tCTTAACCTTTGGCGGCCATCAATTGGCCGCCACCGTGACTACCAAGAGGCGCCGGCAATGTTCCGCGCGTCCACCACCTGATCAGGAGTGCACGATGGACCTGAACC‑‑GTCGGCAGTTCTTCAAGGTCGCCGCCGTCGGCCTTGGAgg > 4:112942/1‑148 (MQ=255) ttAACCTTTGGCGGCCATCAATTGGCCGCCACCGTGACTACCAAGAGGCGCCGGCAATGTTCCGCGCGTCCACCACCTGATCAGGAGTGCACGATGGACCTGAACC‑‑GTCGGCAGTTCTTCAAGGTCGCCGCCGTCGGCCTTGGAGGCTc > 5:245396/1‑149 (MQ=255) aCCTTTGGCGGCCATCAATTGGCCGCCACCGTGACTACCAAGAGGCGCCGGCAATGTTCCGCGCGTCCACCACCTGATCAGGAGTGCACGATGGACCTGAACC‑‑GTCGGCAGTTCTTCAAGGTCGCCGCCGTCGGCCTTGGAGGCTCGa < 4:83448/148‑1 (MQ=255) tttGGCGGCCATCAATTGGCCGCCACCGTGACTACCAAGAGGCGCCGGCAATGTTCCGCGCGTCCACCACCTGATCAGGAGTGCACGATGGACCTGAACC‑‑GTCGGCAGTTCTTCAAGGTCGCCGCCGTCGGCCTTGGAGGCTCGAGc < 8:87030/147‑1 (MQ=255) tttGGCGGCCATCAATTGGCCGCCACCGTGACTACCAAGAGGCGCCGGCAATGTTCCGCGCGTCCACCACCTGATCAGGAGTGCACGATGGACCTGAACC‑‑GTCGGCAGTTCTTCAAGGTCGCCGCCGTCGGCCTTGGAGGCTCGAGc > 7:87030/1‑147 (MQ=255) tttGGCGGCCATCAATTGGCCGCCACCGTGACTACCAAGAGGCGCCGGCAATGTTCCGCGCGTCCACCACCTGATCAGGAGTGCACGATGGACCTGAACC‑‑GTCGGCAGTTCTTCAAGGTCGCCGCCGTCGGCCTTGGAGGCTCGAGCCt > 6:135157/1‑149 (MQ=255) tGGCGGCCATCAATTGGCCGCCACCGTGACTACCAAGAGGCGCCGGCAATGTTCCGCGCGTCCACCACCTGATCAGGAGTGCACGATGGACCTGAACC‑‑GTCGGCAGTTCTTCAAGGTCGCCGCCGTCGGCCTTGGAGGCTCGAGCCtt < 1:110864/148‑1 (MQ=255) ccATCAATTGGCCGCCACCGTGACTACCAAGAGGCGCCGGCAATGTTCCGCGCGTCCACCACCTGATCAGGAGTGCACGATGGACCTGAACC‑‑GTCGGCAGTTCTTCAAGGTCGCCGCCGTCGGCCTTGGAGGCTCGAGCCTTGCGGCGt < 6:245396/149‑1 (MQ=255) cATCAATTGGCCGCCACCGTGACTACCAAGAGGCGCCGGCAATGTTCCGCTCGACCACCACCTGATCAGGAGTGCACGATGGACCTCAACC‑‑GTCGGCAGTTCTTCAAGCTCGCCGCCGTCGGCCTTGGAGGCTCGAGCCTTGCGGCGtt > 3:171182/1‑149 (MQ=255) aaTTGGCCGCCACCGTGACTACCAAGAGGCGCCGGCAATGTTCCGCGCGTCCACCACCTGATCAGGAGTGCACGATGGACCTGAACC‑‑GTCGGCAGTTCTTCAAGGTCGCCGCCGTCGGCCTTGGAGGCTCGAGCCTTGCGGCGTTggg < 6:114595/148‑1 (MQ=255) aaTTGGCCGCCACCGTGACTACCAAGAGGCGCCGGCAATGTTCCGCGCGTCCACCACCTGATCAGGAGTGCACGATGGACCTGAACC‑‑GTCGGCAGTTCTTCAAGGTCGCCGCCGTCGGCCTTGGAGGCTCGAGCCTTGCGGCGTTGGGc < 4:228235/149‑1 (MQ=255) aTTGGCCGCCACCGTGACTACCAAGAGGCGCCGGCAATGTTCCGCGCGTCCACCACCTGATCAGGAGTGCACGATGGACCTGAACC‑‑GTCGGCAGTTCTTCAAGGTCGCCGCCGTCGGCCTTGGAGGCTCGAGCCTTGCGGCGTTGGGCa > 5:120116/1‑149 (MQ=255) cgccACCGTGACTACCAAGAGGCGCCGGCAATGTTCCGCGCGTCCACCACCTGATCAGGAGTGCACGATGGACCTGAACC‑‑GTCGGCAGTTCTTCAAGGTCGCCGCCGTCGGCCTTGGAGGCTCGAGCCATGCGGCGTTGGGCATGGccc > 2:74384/1‑149 (MQ=255) gACTACCAAGAGGCGCCGGCAATGTTCCGCGCGTCCACCACCTGATCAGGAGTGCACGATGGACCTGAACC‑‑GTCGGCAGTTCTTCAAGGTCGCCGCCGTCGGCCTTGGAGGCTCGa > 6:33459/1‑116 (MQ=255) gACTACCAAGAGGCGCCGGCAATGTTCCGCGCGTCCACCACCTGATCAGGAGTGCACGATGGACCTGAACC‑‑GTCGGCAGTTCTTCAAGGTCGCCGCCGTCGGCCTTGGAGGCTCGa < 5:33459/116‑1 (MQ=255) ccAAGAGGCGCCGGCAATGTTCCGCGCGCCCACCACCTGATCAGGAGTGCACGATGGACCTGAACCGTGTCGGCAGTTATTCAAGGTCGCCGCCGTCGGCCTTGGAGGCTCGAGCCTTGCGGCGTTGGGCATGGCCCCAACGCCGGCAt > 1:215929/1‑149 (MQ=255) cAAGAGGCGCCGGCAATGTTCCGCGCGTCCACCACCTGATCAGGAGTGCACGATGGACCTGAACC‑‑GTCGGCAGTTCTTCAAGGTCGCCGCCGTCGGCCTTGGAGGCTCGAGCCTTGCGGCGTTGGGCATGGCCCCAACGCCGGCATTCg > 7:102245/1‑149 (MQ=255) aaGAGGCGCCGGCAATGTTCCGCGCGTCCACCACCTGATCAGGAGTGCACGATGGACCTGAACC‑‑GTCGGCAGTTCTTCAAGGTCGCCGCCGTCGGCCTTGGAGGCTCGAGCCTTGCGGCGTTGGGCATGGCCCCAACGCCGGCATTCGc < 1:105347/149‑1 (MQ=255) gcCGGCAATGTTCCGCGCGTCCACCACCTGATCAGGAGTGCACGATGGACCTGAACC‑‑GTCGGCAGTTCTTCAAGGTCGCCGCCGTCGGCCTTGGAGGCTCGAGCCTTGCGGCGTTGGGCATGGCCCCCACGCCGGCATTCGCCGAGCAg > 5:5097/1‑149 (MQ=255) ggCAATGTTCCGCGCGTCCACCACCTGATCAGGAGTGCACGATGGACCTGAACC‑‑GTCGGCAGTTCTTCAAGGTCGCCGCCGTCGGCCTTGGAGGCTCGAGCCTTGCGGCGTTGGGCATGGc < 6:165729/121‑1 (MQ=255) ggCAATGTTCCGCGCGTCCACCACCTGATCAGGAGTGCACGATGGACCTGAACC‑‑GTCGGCAGTTCTTAAAGGTCGCCGCCGTCGGCCTTGGAGGCTCGAGCCTTGCGGCGTTGGGCATGGc > 5:165729/1‑121 (MQ=255) cgcgTCCACCACCTGATCAGGAGTGCACGATGGACCTGAACC‑‑GTCGGCAGTTCTTCAAGGTCGCCGCCGTCGGCCTAGGAGGCTCGAGCCTTGCGGCGTAGGGCATGGCCCCACCGCCCGCATTCGCCCAGCAGGTGCGTCCCTTCAAg > 8:135740/1‑149 (MQ=255) gTCCACCACCTGATCAGGAGTGCACGATGGACCTGAACC‑‑GTCGGCAGTTCTTCAAGGTCGCCGCCGTCGGCCTTGGAGGCTCGAGCCTTGCGGCGTTGGGCATGGCCCCAACGCCGGCATTCGCCGAGCAGGTGCGTCACTTCAAGCTg < 6:233876/149‑1 (MQ=255) accaccTGATCAGGAGTGCACGATGGACCTGAACC‑‑GTCGGCAGTTCTTCAAGGTCGCCGCCGTCGGCCTTGGAGGCTCGAGCCTTGCGGCGTTGGGCATGGCCCCAACGCCGGCATTCGCCGAGCAGGTGCGTCACTTCAAGCTGgcgc < 6:120116/149‑1 (MQ=255) accaccTGATCAGGAGTGCACGATGGACCTGAACC‑‑GTCGGCAGTTCTTCAAGGTCGCCGCCGTCGGCCTTGGAGGCTCGAGCCTTGCGGCGTTGGGCATGGCCCCAACGCCGGCATTCGCCGAGCAGGTGCGTCACTTCAAGCTGgcg > 6:95546/1‑148 (MQ=255) accaccTGATCAGGAGTGCACGATGGACCTGAACC‑‑GTCGGCAGTTCTTCAAGGTCGCCGCCGTCGGCCTTGGAGGCTCGAGCCTTGCCGCGTTGGTCATGGCCCCCACGCCGGCATTCGCCGAGCAGGTGAGTCACTTAAAGCTGgcg > 8:230599/1‑148 (MQ=255) tGATCAGGAGTGCACGATGGACCTGAACC‑‑GTCGGCAGTTCTTCAAGGTCGCCGCCGTCGGCCTTGGAGGCTCGAGCCTTGCGGCGTTGGGCATGGCCCCAACGCCGGCATTCGCCGAGCAGGTGCGTc < 1:98720/128‑1 (MQ=255) tGATCAGGAGTGCACGATGGACCTGAACC‑‑GTCGGCAGTTCTTCAAGGTCGCCGCCGTCGGCCTTGGAGGCTCGAGCCTTGCGGCGTTGGGCATGGCCCCAACGCCGGCATTCGCCGAGCAGGTGCGTc > 2:98720/1‑128 (MQ=255) aGGAGTGCACGATGGACCTGAACC‑‑GTCGGCAGTTCTTCAAGGTCTCCGCCGTCGGCCTTGGAGGCTCGAGCCTTGCGGCGTTGGGCATGGCCCCAACGCCGGCATTCGCCGAGCAGGTGCGTCAATTCAAGCTGGAGCATACCAAAGa > 6:270597/1‑148 (MQ=255) gTGCACGATGGACCTGAACC‑‑GTCGGCAGTTCTTCAAGGTCGCCGCCGTCGGCCTTGGAGGCTCGAGCCTTGCGGCGTTGGGCATGGCCCCAACGCCGGCATTCGCCGAGCAGGTGCGTCACTTCAAGCTGGCGCATACCAAAGa < 5:210927/144‑1 (MQ=255) gTGCACGATGGACCTGAACC‑‑GTCGGCAGTTCTTCAAGGTCGCCGCCGTCGGCCTTGGAGGCTCGAGCCTTGCGGCGTTGGGCATGGCCCCAACGCCGGCATTCGCCGAGCAGGTGCGTCACTTCAAGCTGGCGCATACCAAAGa > 6:210927/1‑144 (MQ=255) cACGATGGACCTGAACC‑‑GTCGGCAGTTCTTCAAGGTCGCCGCCGTCGGCCTTGGAGGCTCGAGCCTTGCGGCGTTGGGCATGGCCCCAACGCCGGCATTCGCCGAGCAGGTGCGTCACTTCAAGCTGGCGCATACCAAAGAAACCCGt > 5:226627/1‑148 (MQ=255) cACCATGGACCTGAACC‑‑GTCGGCAGTTCTTCAAGGTCGCCGCCGTCGGCCTTGGAGGCTCGAGCCTTGCGGCGTTCGGCATGGCCCCAACGCCGGCATTCCCCGAGCACCTGCGTCACTTCAAGCTGGCGCATACCAAAGAAACCCGt > 5:226625/1‑148 (MQ=255) aTGGACCTGAACC‑‑GTCGGGAGTTCTTCTAGGTCGCCGCCGTGGGCCTTGGAGGCTCGAGCCTTGCGGCGTTGGGCATGGCCCCAACGCCGGCATTCGCCGAGCAGGTGCGTCACTTCAAGCTGGCGCATACCAAAGAAACCCGTAagac < 6:226625/149‑1 (MQ=255) aTGGACCTGAACC‑‑GTCGGCAGTTCTTCAAGGTCGCCGCCGTCGGCCTTGGAGGCTCGAGCCTTGCGGCGTTGGGCATGGCCCCAACGCCGGCATTCGCCGAGCAGGTGCGTCACTTCAAGCTGGCGCATACCAAAGAAACCCGTAacac < 6:226627/149‑1 (MQ=255) aCCTGAACC‑‑GTCGGCAGTTCTTCAAGGTCGCCGCCGTCGGCCTTGGAGGCTCGAGCCTTGCGGCGTTGGGCATGGCCCCAACGCCGGCATTCGCCGAGCAGGTGCGTCACTTCAAGCTGGCGCATACCAAAGAAACCCGTAACACCTGc < 5:135157/149‑1 (MQ=255) cTGAACC‑‑GTCGGCAGTTCTTCAAGGTCGCCCCCGTCGGCCTTGGAGGCTCGAGCCTTGCGGCGTTGGGCATGGCCCCAACGCCGGCATTCGCCGAGCAGGTGCGTCACTTCAAGCTGGCGCATACCAAAGAAACCCGTAACACCTGccc > 1:24000/1‑149 (MQ=255) aaCC‑‑GTCGGCAGTTCTTCAAGGTCGCCGCCGTCGGCCTTGGAGGCTCGAGCCTTGCGGCGTTGGGCATGGCCCCAACGCCGGCATTCGCCGAGCAGGTGCGTCACTTCAAGCTGGCGCATACCAAAGAAACCCGTAACACCTGcccc < 3:112942/147‑1 (MQ=255) aaCC‑‑GTCGGCAGTTCTTCAAGGTCGACGCCGTCGGCCTTGGAGGCTCGAGCCTTGCGGCGTTGGGCATTGACCCAACGCCGTCATTCGCCGAGCAGGTGCGTCACTTCAATCTGGCGCATACCAAAGAAACCCGTAACACCTGCCCCTa < 5:270597/149‑1 (MQ=255) tCGGCAGTTCTTCAAGGTCGCCGCCGTCGGCCTTGGAGGCTCGAGCCTTGCGGCGTTGGGCATGGCCCCAACGCCGGCATTCGCCGAGCAGGTGCGTCACTTCAAGCTGGCGCATACCAAAGAAAcc < 4:126246/127‑1 (MQ=255) tCGGCAGTTCTTCAAGGTCGCCGCCGTCGGCCTTGGAGGCTCGAGCCTTGCGGCGTTGGGCATGGCCCCAACGCCGGCATTCGCCGAGCAGGTGCGTCACTTCAAGCTGGCGCATACCAAAGAAAcc > 3:126246/1‑127 (MQ=255) cAGTTCTTCAAGGTCGCCGCCGTCGGCCTTGGAGGCTCGAGCCTTGCGGCGTTGGGCATGGCCCCAACGCCGGCATTCGCCGAGCAGGTGCGTCACTTCAAGCTGGCGCATACCAAAGAAACCCGTAACACCTGCCCCTACTGTtcggt < 2:215929/149‑1 (MQ=255) ctccAAGGCCGACGGCGGCGACCTTGGAGGCTCGAGCCTTGCGGCGTTGGGCATGGCCCCAACGCCGGCATTCGCCGAGCAGGTGCGTCACTTCAAGCTGGCGCATACCAAAGAAACCCGTAACACCTGccc > 7:719/4‑132 (MQ=255) ctccAAGGCCGACGGCGGCGACCTTGGAGGCTCGAGCCTTGCGGCGTTGGGCATGGCCCCAACGCCGGCATTCGCCGAGCAGGTGCGTCACTTCAAGCTGGCGCATACCAAAGAAACCCGTAACACCTGccc < 8:719/129‑1 (MQ=255) ttcAAGGTCGCCGCCGTCGGCCTTGGAGGCTCGAGCCTTGCGGCGTTGGGCATGGCCCCAACGCCGGCATTCGCCGAGCAGGTGCGTCACTTCAAGCTGGCGCATACCAAAGAAACCCGTAACACCTGCCCCTACTGTTCGGTCGGCTg > 2:12402/1‑149 (MQ=255) tccAAGGCCGACGGCGGCGACCTTGAAGGCTCGAGCCTTGCGGCGTTGGGCATGGCCCCAACGCCGGCATTCGCCGAGCAGGTGCGTCACTTCaa > 6:192858/3‑95 (MQ=37) tccAAGGCCGACGGCGGCGACCTTGAAGGCTCGAGCCTTGCGGCGTTGGGCATGGCCCCAACGCCGGCATTCGCCGAGCAGGTGCGTCACTTCaa < 5:192858/93‑1 (MQ=37) cAAGGTCGCCGCCGTCGGCCTTGGAGGCTCGAGCCTTGCGGCGTTGGGCATGGCCCCAACGCCGGCATTCGCCGAGCAGGTGCGTCACTTCAAg > 4:47060/1‑94 (MQ=255) cAAGGTCGCCGCCGTCGGCCTTGGAGGCTCGAGCCTTGCGGCGTTGGGCATGGCCCCAACGCCGGCATTCGCCGAGCAGGTGCGTCACTTCAAg < 3:47060/94‑1 (MQ=255) cAAGGTCGCCGCCGTCGGCCTTGGAGGCTCGAGCCTTGCGGCGTTGGGCATGGCCCCAACGCCGGCATTCGCCGAGCAGGTGCGTCACTTCAAGCTGGCGCATACCAAAGAAACCCGTAACACCTGCCCCTACTGTTCGGTCGGCTGCg > 5:40525/1‑149 (MQ=255) aGGTCGCCGCCGTCGGCCTTGGAGGCTCGAGCCTTGCGGCGTTGGGCATGGCCCCAACGCCGGCATTCGCCGAGCAGGTGCGTCACTTCAAGCTGGCGCATACCAAAGAAACCCGTAACACCTGCCCCTACTGTTCGGTCGGCTGCGGc < 2:24000/149‑1 (MQ=255) aGGTCGCCGCCGTCGGCCTTGGAGGCTCGAGCCTTGCGGCGTTGGGCATGGCCCCAACGCCGGCATTCGCCGAGCAGGTGCGTCACTTCAAGCTGGCGCATACCAAAGAAACCCGTAACACCTGCCCCTACTGTTCGGTCGGCTGCGGc < 3:173174/149‑1 (MQ=255) gTCGCCGCCGTCGGCCTTGGAGGCTCGAGCCTTGCGGCGTTGGGCATGGCCCCAACGCCGGCATTCGCCGAGCAGGTGCGTCACTTCAAGCTGGCGCATACCAAAGAAACCCGTAACACCTGCCCCTACTGTTCGGTCGGCTGCGGCCt < 6:40525/149‑1 (MQ=255) ccgccgGCGGCCTTGGAGGCTCGAGCCTTGCGGCGTTGGGCATGGCCCCAACGCCGGCATTCGCCGAGCAGGTGCGTCACTTCAAGCTGGCGCATACCAAAGAAACCCGTAACACCTGCCCCTACTGTTCGGTCGGCTGCGGCCTGATc < 7:230599/149‑1 (MQ=255) | ATTCTTGACTAGTCTTAACCTTTGGCGGCCATCAATTGGCCGCCACCGTGACTACCAAGAGGCGCCGGCAATGTTCCGCGCGTCCACCACCTGATCAGGAGTGCACGATGGACCTGAACC‑‑GTCGGCAGTTCTTCAAGGTCGCCGCCGTCGGCCTTGGAGGCTCGAGCCTTGCGGCGTTGGGCATGGCCCCAACGCCGGCATTCGCCGAGCAGGTGCGTCACTTCAAGCTGGCGCATACCAAAGAAACCCGTAACACCTGCCCCTACTGTTCGGTCGGCTGCGGCCTGATC > NC_002947/574857‑575146 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |