Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	575,007	G→A	7.0%	G15D (GGC→GAC)	fdoG →	formate dehydrogenase‑O major subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	575,007	0	G	A	7.0%	165.0 / 2.2	57	G15D (GGC→GAC)	fdoG	formate dehydrogenase‑O major subunit
Reads supporting (aligned to +/- strand):  ref base G (26/27);  new base A (2/2);  total (28/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.11e-01

GACTAGTCTTAACCTTTGGCGGCCATCAATTGGCCGCCACCGTGACTACCAAGAGGCGCCGGCAATGTTCCGCGCGTCCACCACCTGATCAGGAGTGCACGATGGACCTGAACC‑‑GTCGGCAGTTCTTCAAGGTCGCCGCCGTCGGCCTTGGAGGCTCGAGCCTTGCGGCGTTGGGCATGGCCCCAACGCCGGCATTCGCCGAGCAGGTGCGTCACTTCAAGCTGGCGCATACCAAAGAAACCCGTAACACCTGCCCCTACTGTTCGGTCGGCTGCGGCCTGATC  >  NC_002947/574863‑575146                                                                                                                                                   |                                                                                                                                            gACTAGTCTTAACCTTTGGCGGCCATCAATTGGCCGCCACCGTGACTACCAAGAGGCGCCGGCAATGTTCCGCGCGTCCACCACCTGATCAGGAGTGCACGATGGACCTGAACC‑‑GTCGGCAGTTCTTCAAGGTCGCCGCCGTCGGCCt                                                                                                                                          <  2:230565/148‑1 (MQ=255)  aCTAGTCTTAACCTTTGGCGGCCATCAATTGGCCGCCACCGTGACTACCAACAGGCGCCGGCAATGTTCCGCGCGTCCACCACCTGATCAGGAGTGCACGATGGACCTGAACC‑‑GTCGGCAGTTCTTCAAGGTCGCCGCCGTCGGCCtt                                                                                                                                         <  2:48857/148‑1 (MQ=255)      gTCTTAACCTTTGGCGGCCATCAATTGGCCGCCACCGTGACTACCAAGAGGCGCCGGCAATGTTCCGCGCGTCCACCACCTGATCAGGAGTGCACGATGGACCTGAACC‑‑GTCGGCAGTTCTTCAAGGTCGCCGCCGTCGGCCTTGGAgg                                                                                                                                    >  5:114595/1‑149 (MQ=255)       tCTTAACCTTTGGCGGCCATCAATTGGCCGCCACCGTGACTACCAAGAGGCGCCGGCAATGTTCCGCGCGTCCACCACCTGATCAGGAGTGCACGATGGACCTGAACC‑‑GTCGGCAGTTCTTCAAGGTCGCCGCCGTCGGCCTTGGAgg                                                                                                                                    >  4:112942/1‑148 (MQ=255)         ttAACCTTTGGCGGCCATCAATTGGCCGCCACCGTGACTACCAAGAGGCGCCGGCAATGTTCCGCGCGTCCACCACCTGATCAGGAGTGCACGATGGACCTGAACC‑‑GTCGGCAGTTCTTCAAGGTCGCCGCCGTCGGCCTTGGAGGCTc                                                                                                                                 >  5:245396/1‑149 (MQ=255)            aCCTTTGGCGGCCATCAATTGGCCGCCACCGTGACTACCAAGAGGCGCCGGCAATGTTCCGCGCGTCCACCACCTGATCAGGAGTGCACGATGGACCTGAACC‑‑GTCGGCAGTTCTTCAAGGTCGCCGCCGTCGGCCTTGGAGGCTCGa                                                                                                                               <  4:83448/148‑1 (MQ=255)               tttGGCGGCCATCAATTGGCCGCCACCGTGACTACCAAGAGGCGCCGGCAATGTTCCGCGCGTCCACCACCTGATCAGGAGTGCACGATGGACCTGAACC‑‑GTCGGCAGTTCTTCAAGGTCGCCGCCGTCGGCCTTGGAGGCTCGAGc                                                                                                                             <  8:87030/147‑1 (MQ=255)               tttGGCGGCCATCAATTGGCCGCCACCGTGACTACCAAGAGGCGCCGGCAATGTTCCGCGCGTCCACCACCTGATCAGGAGTGCACGATGGACCTGAACC‑‑GTCGGCAGTTCTTCAAGGTCGCCGCCGTCGGCCTTGGAGGCTCGAGc                                                                                                                             >  7:87030/1‑147 (MQ=255)               tttGGCGGCCATCAATTGGCCGCCACCGTGACTACCAAGAGGCGCCGGCAATGTTCCGCGCGTCCACCACCTGATCAGGAGTGCACGATGGACCTGAACC‑‑GTCGGCAGTTCTTCAAGGTCGCCGCCGTCGGCCTTGGAGGCTCGAGCCt                                                                                                                           >  6:135157/1‑149 (MQ=255)                 tGGCGGCCATCAATTGGCCGCCACCGTGACTACCAAGAGGCGCCGGCAATGTTCCGCGCGTCCACCACCTGATCAGGAGTGCACGATGGACCTGAACC‑‑GTCGGCAGTTCTTCAAGGTCGCCGCCGTCGGCCTTGGAGGCTCGAGCCtt                                                                                                                          <  1:110864/148‑1 (MQ=255)                       ccATCAATTGGCCGCCACCGTGACTACCAAGAGGCGCCGGCAATGTTCCGCGCGTCCACCACCTGATCAGGAGTGCACGATGGACCTGAACC‑‑GTCGGCAGTTCTTCAAGGTCGCCGCCGTCGGCCTTGGAGGCTCGAGCCTTGCGGCGt                                                                                                                   <  6:245396/149‑1 (MQ=255)                        cATCAATTGGCCGCCACCGTGACTACCAAGAGGCGCCGGCAATGTTCCGCTCGACCACCACCTGATCAGGAGTGCACGATGGACCTCAACC‑‑GTCGGCAGTTCTTCAAGCTCGCCGCCGTCGGCCTTGGAGGCTCGAGCCTTGCGGCGtt                                                                                                                  >  3:171182/1‑149 (MQ=255)                            aaTTGGCCGCCACCGTGACTACCAAGAGGCGCCGGCAATGTTCCGCGCGTCCACCACCTGATCAGGAGTGCACGATGGACCTGAACC‑‑GTCGGCAGTTCTTCAAGGTCGCCGCCGTCGGCCTTGGAGGCTCGAGCCTTGCGGCGTTggg                                                                                                               <  6:114595/148‑1 (MQ=255)                            aaTTGGCCGCCACCGTGACTACCAAGAGGCGCCGGCAATGTTCCGCGCGTCCACCACCTGATCAGGAGTGCACGATGGACCTGAACC‑‑GTCGGCAGTTCTTCAAGGTCGCCGCCGTCGGCCTTGGAGGCTCGAGCCTTGCGGCGTTGGGc                                                                                                              <  4:228235/149‑1 (MQ=255)                             aTTGGCCGCCACCGTGACTACCAAGAGGCGCCGGCAATGTTCCGCGCGTCCACCACCTGATCAGGAGTGCACGATGGACCTGAACC‑‑GTCGGCAGTTCTTCAAGGTCGCCGCCGTCGGCCTTGGAGGCTCGAGCCTTGCGGCGTTGGGCa                                                                                                             >  5:120116/1‑149 (MQ=255)                                   cgccACCGTGACTACCAAGAGGCGCCGGCAATGTTCCGCGCGTCCACCACCTGATCAGGAGTGCACGATGGACCTGAACC‑‑GTCGGCAGTTCTTCAAGGTCGCCGCCGTCGGCCTTGGAGGCTCGAGCCATGCGGCGTTGGGCATGGccc                                                                                                       >  2:74384/1‑149 (MQ=255)                                            gACTACCAAGAGGCGCCGGCAATGTTCCGCGCGTCCACCACCTGATCAGGAGTGCACGATGGACCTGAACC‑‑GTCGGCAGTTCTTCAAGGTCGCCGCCGTCGGCCTTGGAGGCTCGa                                                                                                                               <  5:33459/116‑1 (MQ=255)                                            gACTACCAAGAGGCGCCGGCAATGTTCCGCGCGTCCACCACCTGATCAGGAGTGCACGATGGACCTGAACC‑‑GTCGGCAGTTCTTCAAGGTCGCCGCCGTCGGCCTTGGAGGCTCGa                                                                                                                               >  6:33459/1‑116 (MQ=255)                                                 ccAAGAGGCGCCGGCAATGTTCCGCGCGCCCACCACCTGATCAGGAGTGCACGATGGACCTGAACCGTGTCGGCAGTTATTCAAGGTCGCCGCCGTCGGCCTTGGAGGCTCGAGCCTTGCGGCGTTGGGCATGGCCCCAACGCCGGCAt                                                                                           >  1:215929/1‑149 (MQ=255)                                                  cAAGAGGCGCCGGCAATGTTCCGCGCGTCCACCACCTGATCAGGAGTGCACGATGGACCTGAACC‑‑GTCGGCAGTTCTTCAAGGTCGCCGCCGTCGGCCTTGGAGGCTCGAGCCTTGCGGCGTTGGGCATGGCCCCAACGCCGGCATTCg                                                                                        >  7:102245/1‑149 (MQ=255)                                                   aaGAGGCGCCGGCAATGTTCCGCGCGTCCACCACCTGATCAGGAGTGCACGATGGACCTGAACC‑‑GTCGGCAGTTCTTCAAGGTCGCCGCCGTCGGCCTTGGAGGCTCGAGCCTTGCGGCGTTGGGCATGGCCCCAACGCCGGCATTCGc                                                                                       <  1:105347/149‑1 (MQ=255)                                                          gcCGGCAATGTTCCGCGCGTCCACCACCTGATCAGGAGTGCACGATGGACCTGAACC‑‑GTCGGCAGTTCTTCAAGGTCGCCGCCGTCGGCCTTGGAGGCTCGAGCCTTGCGGCGTTGGGCATGGCCCCCACGCCGGCATTCGCCGAGCAg                                                                                >  5:5097/1‑149 (MQ=255)                                                             ggCAATGTTCCGCGCGTCCACCACCTGATCAGGAGTGCACGATGGACCTGAACC‑‑GTCGGCAGTTCTTCAAGGTCGCCGCCGTCGGCCTTGGAGGCTCGAGCCTTGCGGCGTTGGGCATGGc                                                                                                         <  6:165729/121‑1 (MQ=255)                                                             ggCAATGTTCCGCGCGTCCACCACCTGATCAGGAGTGCACGATGGACCTGAACC‑‑GTCGGCAGTTCTTAAAGGTCGCCGCCGTCGGCCTTGGAGGCTCGAGCCTTGCGGCGTTGGGCATGGc                                                                                                         >  5:165729/1‑121 (MQ=255)                                                                         cgcgTCCACCACCTGATCAGGAGTGCACGATGGACCTGAACC‑‑GTCGGCAGTTCTTCAAGGTCGCCGCCGTCGGCCTAGGAGGCTCGAGCCTTGCGGCGTAGGGCATGGCCCCACCGCCCGCATTCGCCCAGCAGGTGCGTCCCTTCAAg                                                                 >  8:135740/1‑149 (MQ=255)                                                                            gTCCACCACCTGATCAGGAGTGCACGATGGACCTGAACC‑‑GTCGGCAGTTCTTCAAGGTCGCCGCCGTCGGCCTTGGAGGCTCGAGCCTTGCGGCGTTGGGCATGGCCCCAACGCCGGCATTCGCCGAGCAGGTGCGTCACTTCAAGCTg                                                              <  6:233876/149‑1 (MQ=255)                                                                                accaccTGATCAGGAGTGCACGATGGACCTGAACC‑‑GTCGGCAGTTCTTCAAGGTCGCCGCCGTCGGCCTTGGAGGCTCGAGCCTTGCGGCGTTGGGCATGGCCCCAACGCCGGCATTCGCCGAGCAGGTGCGTCACTTCAAGCTGgcgc                                                          <  6:120116/149‑1 (MQ=255)                                                                                accaccTGATCAGGAGTGCACGATGGACCTGAACC‑‑GTCGGCAGTTCTTCAAGGTCGCCGCCGTCGGCCTTGGAGGCTCGAGCCTTGCGGCGTTGGGCATGGCCCCAACGCCGGCATTCGCCGAGCAGGTGCGTCACTTCAAGCTGgcg                                                           >  6:95546/1‑148 (MQ=255)                                                                                accaccTGATCAGGAGTGCACGATGGACCTGAACC‑‑GTCGGCAGTTCTTCAAGGTCGCCGCCGTCGGCCTTGGAGGCTCGAGCCTTGCCGCGTTGGTCATGGCCCCCACGCCGGCATTCGCCGAGCAGGTGAGTCACTTAAAGCTGgcg                                                           >  8:230599/1‑148 (MQ=255)                                                                                      tGATCAGGAGTGCACGATGGACCTGAACC‑‑GTCGGCAGTTCTTCAAGGTCGCCGCCGTCGGCCTTGGAGGCTCGAGCCTTGCGGCGTTGGGCATGGCCCCAACGCCGGCATTCGCCGAGCAGGTGCGTc                                                                         >  2:98720/1‑128 (MQ=255)                                                                                      tGATCAGGAGTGCACGATGGACCTGAACC‑‑GTCGGCAGTTCTTCAAGGTCGCCGCCGTCGGCCTTGGAGGCTCGAGCCTTGCGGCGTTGGGCATGGCCCCAACGCCGGCATTCGCCGAGCAGGTGCGTc                                                                         <  1:98720/128‑1 (MQ=255)                                                                                           aGGAGTGCACGATGGACCTGAACC‑‑GTCGGCAGTTCTTCAAGGTCTCCGCCGTCGGCCTTGGAGGCTCGAGCCTTGCGGCGTTGGGCATGGCCCCAACGCCGGCATTCGCCGAGCAGGTGCGTCAATTCAAGCTGGAGCATACCAAAGa                                                >  6:270597/1‑148 (MQ=255)                                                                                               gTGCACGATGGACCTGAACC‑‑GTCGGCAGTTCTTCAAGGTCGCCGCCGTCGGCCTTGGAGGCTCGAGCCTTGCGGCGTTGGGCATGGCCCCAACGCCGGCATTCGCCGAGCAGGTGCGTCACTTCAAGCTGGCGCATACCAAAGa                                                <  5:210927/144‑1 (MQ=255)                                                                                               gTGCACGATGGACCTGAACC‑‑GTCGGCAGTTCTTCAAGGTCGCCGCCGTCGGCCTTGGAGGCTCGAGCCTTGCGGCGTTGGGCATGGCCCCAACGCCGGCATTCGCCGAGCAGGTGCGTCACTTCAAGCTGGCGCATACCAAAGa                                                >  6:210927/1‑144 (MQ=255)                                                                                                  cACGATGGACCTGAACC‑‑GTCGGCAGTTCTTCAAGGTCGCCGCCGTCGGCCTTGGAGGCTCGAGCCTTGCGGCGTTGGGCATGGCCCCAACGCCGGCATTCGCCGAGCAGGTGCGTCACTTCAAGCTGGCGCATACCAAAGAAACCCGt                                         >  5:226627/1‑148 (MQ=255)                                                                                                  cACCATGGACCTGAACC‑‑GTCGGCAGTTCTTCAAGGTCGCCGCCGTCGGCCTTGGAGGCTCGAGCCTTGCGGCGTTCGGCATGGCCCCAACGCCGGCATTCCCCGAGCACCTGCGTCACTTCAAGCTGGCGCATACCAAAGAAACCCGt                                         >  5:226625/1‑148 (MQ=255)                                                                                                      aTGGACCTGAACC‑‑GTCGGGAGTTCTTCTAGGTCGCCGCCGTGGGCCTTGGAGGCTCGAGCCTTGCGGCGTTGGGCATGGCCCCAACGCCGGCATTCGCCGAGCAGGTGCGTCACTTCAAGCTGGCGCATACCAAAGAAACCCGTAagac                                    <  6:226625/149‑1 (MQ=255)                                                                                                      aTGGACCTGAACC‑‑GTCGGCAGTTCTTCAAGGTCGCCGCCGTCGGCCTTGGAGGCTCGAGCCTTGCGGCGTTGGGCATGGCCCCAACGCCGGCATTCGCCGAGCAGGTGCGTCACTTCAAGCTGGCGCATACCAAAGAAACCCGTAacac                                    <  6:226627/149‑1 (MQ=255)                                                                                                          aCCTGAACC‑‑GTCGGCAGTTCTTCAAGGTCGCCGCCGTCGGCCTTGGAGGCTCGAGCCTTGCGGCGTTGGGCATGGCCCCAACGCCGGCATTCGCCGAGCAGGTGCGTCACTTCAAGCTGGCGCATACCAAAGAAACCCGTAACACCTGc                                <  5:135157/149‑1 (MQ=255)                                                                                                            cTGAACC‑‑GTCGGCAGTTCTTCAAGGTCGCCCCCGTCGGCCTTGGAGGCTCGAGCCTTGCGGCGTTGGGCATGGCCCCAACGCCGGCATTCGCCGAGCAGGTGCGTCACTTCAAGCTGGCGCATACCAAAGAAACCCGTAACACCTGccc                              >  1:24000/1‑149 (MQ=255)                                                                                                               aaCC‑‑GTCGGCAGTTCTTCAAGGTCGCCGCCGTCGGCCTTGGAGGCTCGAGCCTTGCGGCGTTGGGCATGGCCCCAACGCCGGCATTCGCCGAGCAGGTGCGTCACTTCAAGCTGGCGCATACCAAAGAAACCCGTAACACCTGcccc                             <  3:112942/147‑1 (MQ=255)                                                                                                               aaCC‑‑GTCGGCAGTTCTTCAAGGTCGACGCCGTCGGCCTTGGAGGCTCGAGCCTTGCGGCGTTGGGCATTGACCCAACGCCGTCATTCGCCGAGCAGGTGCGTCACTTCAATCTGGCGCATACCAAAGAAACCCGTAACACCTGCCCCTa                           <  5:270597/149‑1 (MQ=255)                                                                                                                      tCGGCAGTTCTTCAAGGTCGCCGCCGTCGGCCTTGGAGGCTCGAGCCTTGCGGCGTTGGGCATGGCCCCAACGCCGGCATTCGCCGAGCAGGTGCGTCACTTCAAGCTGGCGCATACCAAAGAAAcc                                            <  4:126246/127‑1 (MQ=255)                                                                                                                      tCGGCAGTTCTTCAAGGTCGCCGCCGTCGGCCTTGGAGGCTCGAGCCTTGCGGCGTTGGGCATGGCCCCAACGCCGGCATTCGCCGAGCAGGTGCGTCACTTCAAGCTGGCGCATACCAAAGAAAcc                                            >  3:126246/1‑127 (MQ=255)                                                                                                                          cAGTTCTTCAAGGTCGCCGCCGTCGGCCTTGGAGGCTCGAGCCTTGCGGCGTTGGGCATGGCCCCAACGCCGGCATTCGCCGAGCAGGTGCGTCACTTCAAGCTGGCGCATACCAAAGAAACCCGTAACACCTGCCCCTACTGTtcggt                  <  2:215929/149‑1 (MQ=255)                                                                                                                               ctccAAGGCCGACGGCGGCGACCTTGGAGGCTCGAGCCTTGCGGCGTTGGGCATGGCCCCAACGCCGGCATTCGCCGAGCAGGTGCGTCACTTCAAGCTGGCGCATACCAAAGAAACCCGTAACACCTGccc                              >  7:719/4‑132 (MQ=255)                                                                                                                               ctccAAGGCCGACGGCGGCGACCTTGGAGGCTCGAGCCTTGCGGCGTTGGGCATGGCCCCAACGCCGGCATTCGCCGAGCAGGTGCGTCACTTCAAGCTGGCGCATACCAAAGAAACCCGTAACACCTGccc                              <  8:719/129‑1 (MQ=255)                                                                                                                                ttcAAGGTCGCCGCCGTCGGCCTTGGAGGCTCGAGCCTTGCGGCGTTGGGCATGGCCCCAACGCCGGCATTCGCCGAGCAGGTGCGTCACTTCAAGCTGGCGCATACCAAAGAAACCCGTAACACCTGCCCCTACTGTTCGGTCGGCTg            >  2:12402/1‑149 (MQ=255)                                                                                                                                tccAAGGCCGACGGCGGCGACCTTGAAGGCTCGAGCCTTGCGGCGTTGGGCATGGCCCCAACGCCGGCATTCGCCGAGCAGGTGCGTCACTTCaa                                                                  >  6:192858/3‑95 (MQ=37)                                                                                                                                tccAAGGCCGACGGCGGCGACCTTGAAGGCTCGAGCCTTGCGGCGTTGGGCATGGCCCCAACGCCGGCATTCGCCGAGCAGGTGCGTCACTTCaa                                                                  <  5:192858/93‑1 (MQ=37)                                                                                                                                  cAAGGTCGCCGCCGTCGGCCTTGGAGGCTCGAGCCTTGCGGCGTTGGGCATGGCCCCAACGCCGGCATTCGCCGAGCAGGTGCGTCACTTCAAg                                                                 >  4:47060/1‑94 (MQ=255)                                                                                                                                  cAAGGTCGCCGCCGTCGGCCTTGGAGGCTCGAGCCTTGCGGCGTTGGGCATGGCCCCAACGCCGGCATTCGCCGAGCAGGTGCGTCACTTCAAg                                                                 <  3:47060/94‑1 (MQ=255)                                                                                                                                  cAAGGTCGCCGCCGTCGGCCTTGGAGGCTCGAGCCTTGCGGCGTTGGGCATGGCCCCAACGCCGGCATTCGCCGAGCAGGTGCGTCACTTCAAGCTGGCGCATACCAAAGAAACCCGTAACACCTGCCCCTACTGTTCGGTCGGCTGCg          >  5:40525/1‑149 (MQ=255)                                                                                                                                    aGGTCGCCGCCGTCGGCCTTGGAGGCTCGAGCCTTGCGGCGTTGGGCATGGCCCCAACGCCGGCATTCGCCGAGCAGGTGCGTCACTTCAAGCTGGCGCATACCAAAGAAACCCGTAACACCTGCCCCTACTGTTCGGTCGGCTGCGGc        <  2:24000/149‑1 (MQ=255)                                                                                                                                    aGGTCGCCGCCGTCGGCCTTGGAGGCTCGAGCCTTGCGGCGTTGGGCATGGCCCCAACGCCGGCATTCGCCGAGCAGGTGCGTCACTTCAAGCTGGCGCATACCAAAGAAACCCGTAACACCTGCCCCTACTGTTCGGTCGGCTGCGGc        <  3:173174/149‑1 (MQ=255)                                                                                                                                      gTCGCCGCCGTCGGCCTTGGAGGCTCGAGCCTTGCGGCGTTGGGCATGGCCCCAACGCCGGCATTCGCCGAGCAGGTGCGTCACTTCAAGCTGGCGCATACCAAAGAAACCCGTAACACCTGCCCCTACTGTTCGGTCGGCTGCGGCCt      <  6:40525/149‑1 (MQ=255)                                                                                                                                          ccgccgGCGGCCTTGGAGGCTCGAGCCTTGCGGCGTTGGGCATGGCCCCAACGCCGGCATTCGCCGAGCAGGTGCGTCACTTCAAGCTGGCGCATACCAAAGAAACCCGTAACACCTGCCCCTACTGTTCGGTCGGCTGCGGCCTGATc  <  7:230599/149‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                            GACTAGTCTTAACCTTTGGCGGCCATCAATTGGCCGCCACCGTGACTACCAAGAGGCGCCGGCAATGTTCCGCGCGTCCACCACCTGATCAGGAGTGCACGATGGACCTGAACC‑‑GTCGGCAGTTCTTCAAGGTCGCCGCCGTCGGCCTTGGAGGCTCGAGCCTTGCGGCGTTGGGCATGGCCCCAACGCCGGCATTCGCCGAGCAGGTGCGTCACTTCAAGCTGGCGCATACCAAAGAAACCCGTAACACCTGCCCCTACTGTTCGGTCGGCTGCGGCCTGATC  >  NC_002947/574863‑575146

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑