Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 615,566 G→A 8.3% intergenic (‑81/+85) xseB ← / ← ribB exodeoxyribonuclease 7 small subunit/3,4‑dihydroxy‑2‑butanone‑4‑phosphate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947615,5660GA 8.3% 129.9 / 2.5 48intergenic (‑81/+85)xseB/ribBexodeoxyribonuclease 7 small subunit/3,4‑dihydroxy‑2‑butanone‑4‑phosphate synthase
Reads supporting (aligned to +/- strand):  ref base G (24/20);  new base A (2/2);  total (26/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.55e-01

CTCGACCAGTGCTTGCAGGTCTGCGAGGGATTGCTCGAAATCGAGGGAGGCTTTTTTGCGGGCCATGGCGACTGTCTCGGTGGCATTCAGAACGGCGCGACACTAGCAGAGCGGGGCGGGGGGAGCAAACTTCCGGCAGCTGCATCGGCTGATGCAGACCGATGGAGGAGCGGCCCTGTGTCGCGAAAGGGCCGCTCGCACAAGGACCGCAAAGCGTCCCGGTTCGGTGGATCAGGCCGGCTCTGCCTCCCGTTCCAGCATTGCCTCACGGTACCGCGCCAGCTCCTCGATGGT  >  NC_002947/615420‑615713
                                                                                                                                                  |                                                                                                                                                   
cTCGACCAGTGCTTGCAGGTCTGCGAGGGATTGCTCGAAATCGAGGGAGGCTTTTTTGCGGGCCATGGCGACTGTCTCGGTGGCATTCAGAACGGCGCGACACTAGCAGAGCGGGGCGGGGGGAGCAAACTTCCGGCAGCTGCATCGGc                                                                                                                                                   >  6:219197/1‑149 (MQ=255)
   gACCAGTGCTGGCAGGTCTGCGAGGGATTGCTCGACATCGAGGGCGGCGTTTTTGCGGGCCATGGCGACTGTCTCGGTGGCATTCAGAACGGCGCGACACTAGCAGAGCGGGGCGGGGGGAGCAAACTTCCGGCAGCTGCATCGGCTGa                                                                                                                                                <  8:246581/149‑1 (MQ=255)
      cAGTGCTTGCAGGTCTGCGAGGGATTGCTCGAAATCGAGGGAGGCTTTTTTGCGGGCCATGGCGACTGTCTCGGTGGCATTCAGAACGGCGCGACACTAGCAGAGCGGGGCGGGGGGAGCAAACTTCCGGCAGCTGCATCGGCTGATg                                                                                                                                              >  7:252600/1‑148 (MQ=255)
        gTGCTTGCAGGTCTGCGAGGGATTGCTCGAAATCGAGGGAGGCTTTTTTGCGGGCCATGGCGACTGTCTCGGTGGCATTCAGAACGGCGCGACACTAGCAGAGCGGGGCGGGGGGAGCAAACTTCCGGCAGCTGCATCGGCTGATg                                                                                                                                              >  6:82246/1‑146 (MQ=255)
          gCTTGCAGGTCTGCGAGGGATTGCTCGAAATCGAGGGAGGCTTTTTTGCGGGCCATGGCGACTGTCTCGGTGGCATTCAGAACGGCGCGACACTAGCAGAGCGGGGCGGGGGGAGCAAACTTCCGGCAGCTGCATCAGCCGATGCAgc                                                                                                                                          <  4:232954/148‑2 (MQ=255)
                aGGTCTGCGAGGGATTGCGCGAAATCGAGGGAGGCTTTTTTGCGGGCCATGGCGACTGTCTCGGTGGCATTCAGAACGGCGCGACACTAGCAGAGCGGGGCGGGGGGAGCAAACTTCCGGCAGCTGCATCGGCTGATGCAGACCGATgg                                                                                                                                   <  6:22962/149‑1 (MQ=255)
                   tCTGCGAGGGATTGCTCGAAATCGAGGGAGGCTTTTTTGCGGGCCATGGCGACTGTCTCGGTGGCATTCAGAACGGCGCGACACTAGCAGAGCGGGGCTGGGGGAGCAAACTTCCGGCAGCTGCATCGGCTGATGCAGACCGATggag                                                                                                                                 >  1:147341/1‑148 (MQ=255)
                    cTGCGAGGGATTGCTCGAAATCGAGGGAGGCTTTTTTGCGGGCCATGGCGACTGTCTCGGTGGCATTCAGAACGGCGCGACACTAGCAGAGCGGCGCGGGGGGAGCAAACTTCCGGCAGCTGCATCGGCt                                                                                                                                                  >  4:217286/1‑130 (MQ=255)
                        gAGGGATTGCTCGAAATCGAGGGAGGCTTTTTTGCGGGCCATGGCGACTGTCTCGGTGGCATTCAGAACGGCGCGACACTAGCAGAGCGGGGCGGGGGGAGCAAACTTCCGGCAGCTGCATCGGCTGATGCAGACCGATGGAGGAGCgg                                                                                                                           <  5:82246/149‑1 (MQ=255)
                          gggATTGCTCGAAATCGAGGGAGGCTTTTTTGCGGGCCATGGCGACTGTCTCGGTGGCATTCAGAACGGCGCGACACTAGCAGAGCGGGGCGGGGGGAGCAAACTTCCGGCAGCTGCATCgg                                                                                                                                                    <  1:132602/122‑1 (MQ=255)
                          gggATTGCTCGAAATCGAGGGAGGCTTTTTTGCGGGCCATGGCGACTGTCTCGGTGGCATTCAGAACGGCGCGACACTAGCAGAGCGGGGCGGGGGGAGCAAACTTCCGGCAGCTGCATCgg                                                                                                                                                    >  2:132602/1‑122 (MQ=255)
                            gATTGCTCGAAATCGAGGGAGGGTTTTTTGCGGGCCAGGGCGACTGGCTCGGTGGCATTCAGACCGGCGCGACACTAGCAGAGCGGGGCGGGGGGTGCAAACTTCCGGCAGCTGCATCGGCTGATGCAGACCGATGGAGGAGCGGCCCt                                                                                                                       <  5:268409/149‑1 (MQ=255)
                                       aTCGAGGGAGGCTTTTTTGCGGGCCATGGCGACTGTCTCGGTGGCATTCAGAACGGCGCGACACTAGCAGAGCGGGGCGGGGGGAGTAAACTTCCGGCAGCTGCATCGGCTGATGCAGACCGATGGAGGAGCGGCCCTGTGTCGCGaaa                                                                                                            <  2:76599/149‑1 (MQ=255)
                                          gagggaggCTTTTTTGCGGGCCATGGCGACTGTCTCGGTGGCATTCAGAACGGCGCGACACTAGCAGAGCGGGGCGGGGGGAGCAAACTTCCGGCAGCTGCATCGGCTGATGCAGACCGATGGAGGAGCGGCCCTGTGTCGCGAAAgg                                                                                                          <  8:252600/148‑1 (MQ=255)
                                           agggaggCTTTTTTGCGGGCCATGGCGACTGTCTCGGTGGCATTCAGAACGGCGCGACACTAGCAGAGCGGGGCGGGGGGAGCAAACTTCCGGCAGATGCATCGGCTGATGCAGACCGATGGAGGAGCGGCCCTGTGTCGCGAAAGGGc                                                                                                        >  1:25943/1‑149 (MQ=255)
                                                 gCTTTTTTGCGGGCCATGGCGACTGTCTCGGTGGCATTCAGAACGGCGCGACACTAGCAGAGCGGGGCGGGGGGAGCAAACTTCCGGCAGCTGCATCGGCTGATGCAGACCGATGGAGGAGCGGCCCTGTGTCGCGAAAGGGCCGCTCg                                                                                                  >  3:240365/1‑149 (MQ=255)
                                                   ttttttGCGGGCCATGGCGACTGTCTCGGTGGCATTCAGAACGGCGCGACACTAGCAGAGCGGGGCGGGGGGAGCAAACTTCCGGCAGCTGCATCGGCTGATGCAGACCGATGGAGGAGCGGCCCTGTGTCGCGAAAGGGCCGCTCGca                                                                                                >  6:212940/1‑149 (MQ=255)
                                                   ttttttGCGGGCCATGGCGACTGTCTCGGTGGCATTCAGAACGGCGCGACACTAGCAGAGCGGGGCGGGGGGAGCAAACTTCCGGCAGCTGCATCGGCTGATGCAGACCGATGGAGGAGCGGCCCTGTGTCGCGAAAGGGCCGCTCGca                                                                                                >  1:2914/1‑149 (MQ=255)
                                                     ttttGCGGGCCATGGCGACTGTCTCGGTGGCATTCAGAACGGCGCGACACTAGCAGAGCGGGGCGGGGGGAGCAAACTTCCGGCAGCTGCATCGGCTGATGCAGACCGATGGAGGAGCGGCCCTGTGTCGCGAAAGGGCCGCTCGca                                                                                                <  8:1969/147‑1 (MQ=255)
                                                          cGGGCCATGGCGACTGTCTCGGTGGCATTCAGAACGGCGCGACACTAGCAGAGCGGGGCGGGGGGAGCAAACTTCCGGCAGCTGCATCGGCTGATGCAGACCGATGGAGGAGCGGCCCTGTGTCGCGAAAGGGCCGCTCGCACAAGGa                                                                                          <  5:212940/148‑1 (MQ=255)
                                                           gggCCATGGCGACTGTCTCGGTGGCATTCAGAACGGCGCGACACTAGCAGAGCGGGGCGGGGGGAGCAAACTTCCGGCAGCTGCATCGGCTGATGCAGACCGATGGAGGAGCGGCCCTGTGTCGCGAAAGGGCCGCTCGCACAAGGAcc                                                                                        >  6:236697/1‑149 (MQ=255)
                                                              ccATGGCGACTGTCTCGGTGGCATTCAGAACGGCGCGACACTAGCAGAGCGGGGCGGGGGGAGCAAACTTCCGGCAGCTGCATCGGCTGATGCAGACCGATGGAGGAGCGGCCCTGTGTCGCGAAAGGGCCGCTCGCACAAGGACCGc                                                                                      >  7:73923/1‑148 (MQ=255)
                                                                aTGGCGACTGTCTCGGTGGCATTCAGAACGGCGCGACACTAGCAGCGCGGGGCGGGGGGAGCAAACTTCCGGCAGCTGCATCGGCTGATGCAGACCGATGGAGGAGCGGCCCTGTGTCGCGAAAGGGCCGCTCGCACAAGGACCGCaaa                                                                                   <  2:2914/149‑1 (MQ=255)
                                                                                tGGCATTCAGAACGGCGCGACACTAGCAGAGCGGGGCGGGGGGAGCAAACTTCCGGCAGCTGCATCGGCTGATGCAGACCGATGGAGGAGCGGCCCTTTCGCGACACAGGGCCGCt                                                                                                    >  5:109382/1‑116 (MQ=38)
                                                                                tGGCATTCAGAACGGCGCGACACTAGCAGAGCGGGGCGGGGGGAGCAAACTTCCGGCAGCTGCATCGGCTGATGCAGACCGATGGAGGAGCGGCCCTTTCGCGACACAGGGCCGCt                                                                                                    <  6:109382/116‑1 (MQ=38)
                                                                                   cATTCAGAACGGCGCGACACTAGCAGAGCGGGGCGGGGGGAGCAAACTTCCGGCAGCTGCATCGGCTGATGCAGACCGATGGAGGAGCGGCCCTGTGTCGCGAAAGGGCCGCTCGCACAAGGACCGCAAAGCGTCCCGGTTCGGTGGAt                                                                >  1:119610/1‑149 (MQ=255)
                                                                                        aGAACGGCGCGACACTAGCAGAGCGGGGCGGGGGGAGCAAACTTCCGGCAGCTGCATCGGCTGATGCAGACCGATGGAGGAGCGGCCCTGTGTCGCGAAAGGGCCGCTCGCACAAGGACCGCAAAGCGTCCCGGTTCGGTGGATCAGGc                                                           >  1:81434/1‑149 (MQ=255)
                                                                                                  gACACTAGCAGAGCGGGGCGGGGGGAGCAAACTTCCGGCAGCTGCATCGGCTGATGCAGACCGATGGAGGAGCGGCCCTGTGTCGCGAAAGGGCCGCTCGCACAAGGACCGCAAAGCGTCCCGGtt                                                                        <  6:20393/126‑1 (MQ=255)
                                                                                                  gACACTAGCAGAGCGGGGCGGGGGGAGCAAACTTCCGGCAGCTGCATCGGCTGATGCAGACCGATGGAGGAGCGGCCCTGTGTCGCGAAAGGGCCGCTCGCACAAGGACCGCAAAGCGTCCCGGtt                                                                        >  5:20393/1‑126 (MQ=255)
                                                                                                      cTAGCAGAGCGGGGCGGGGGGAGCAAACTTCCGGCAGCTGCATCGGCTGATGCAGACCGATGGAGGAGCGGCCCTGTGTCGCGAAAGGGCCGCTCGCACAAGGACCGCAAAGCGTCCCGGTTCGGTGGATCAGGCCGGCTCTGCCTccc                                             <  5:219197/149‑1 (MQ=255)
                                                                                                       tAGCAGAGCGGGGCGGGGGGAGCAAACTTCCGACAGCTGCATCGGCTGATGCAGACCGATGGAGGAGCGGCCCTGTGTCGCGAAAGGGCCGCTCGCACAAGGACCGCAAAGCGTCCCGGTTCGGTGGATCAGGCCGGCTCTGCCTCCCg                                            >  8:83128/1‑149 (MQ=255)
                                                                                                         gCAGAGCGGGGCGGGGGGAGCAAACTTCCGGCAGCTGCATCGGCTGATGCAGACCGATGGAGGAGCGGCCCTGTGTCGCGAAAGGGCCGCTCGCACAAGGACCGCAAAGCGTCCCGGTTCGGTGGATCAGGCCGGCTCTGCCTCCCGtt                                          >  6:124939/1‑149 (MQ=255)
                                                                                                              gcggggctggGGGAGCAAACTTCCGGCAGCTGCATCGGCTGATGCAGACCGATGGAGGAGCGGCCCTGTGTCGCGAAAGGGCCGCTCGCACAAGGACCGCAAAGCGTCCCGGTTCGGTGGATCAGGCCGGCTCTGCCTCCCGTTCCAGc                                     <  2:147341/149‑1 (MQ=255)
                                                                                                                    cggggGGAGCAAACTTCCGGCAGCTGCATCGGCTGATGCAGACCGATGGAGGAGCGGCCCTGTGTCGCGAAAGGGCCGCTCGCACAAGGACCGCAAAGCGTCCCGGTTCGGTGGATCAGGCCGGCTCTGCCTCCCGTTCCAGCATTGcc                               <  5:124939/149‑1 (MQ=255)
                                                                                                                        gggAGCAAACTTCCGGCAGCTGCATCGGCTGATGCAGACCGATGGAGGAGCGGCCCTGTGTCGCGAAAGGGCCGCTCGCACAAGGACCGCAAAGCGTCCCGGTTCGGTGGATCAGGCCGGCTCTGCCTCCCGTTCCAGCATTGCCTCAc                           <  8:73923/149‑1 (MQ=255)
                                                                                                                        gggAGCAAACTTCCGACAGCTGCATCGGCTGATGCAGACCGATGGAGGAGCGGCCCTGTGTCGCGAAAGGGCCGCTCGCACAAGGACCGCAAAGCGTCCCGGTTCGGTGGATCAGGCCGGCTCTGCCTCCCGTTCCAGCATTGCCTCAc                           <  7:83128/149‑1 (MQ=255)
                                                                                                                            gCAAACTTCCGGCAGCTGCATCGGCTGATGCAGACCGATGGAGGAGCGGCCCTGTGTCGCGAAAGGGCCGCTCGCACAAGGACCGCAAAGCGTccc                                                                            <  4:54653/96‑1 (MQ=255)
                                                                                                                            gCAAACTTCCGGCAGCTGCATCGGCTGATGCAGACCGATGGAGGAGCGGCCCTGTGTCGCGAAAGGGCCGCTCGCACAAGGACCGCAAAGCGTccc                                                                            >  3:54653/1‑96 (MQ=255)
                                                                                                                              aaaCTTCCGGCAGCTGCATCGGCTGATGCAGACCGATGGAGGAGCGGCCCTGTGTCGCGAAAGGGCCGCTCGCACAAGGACCGCAAAGCGTCCCGGTTCGGTGGATCAGGCCGGCTCTGCCTCCCGTTCCAGCATTGCCTCACGGTAcc                     >  5:232664/1‑149 (MQ=255)
                                                                                                                              aaaCTTCCGGCAGCTGCATCGGCTGATGCAGACCGATGGAGGAGCGGCCCTGTGTCGCGAAAGGGCCGCTCGCACAAGGACCGCAAAGCGTCCCGGTTCGGTGGATCAGGCCGGCTCTGCCTCCCGTTCCAGCATTGCCTCACGGTAcc                     >  3:208675/1‑149 (MQ=255)
                                                                                                                                  ttCCGGCAGCTGCATCGGCTGATGCAGACCGATGGAGGAGCGGCCCTGTGTCGCGAAAGGGCCGCTCGCACAAGGACCGCAAAGCGTCCCGGTTCGGTGGATCAGGCCGGCTCTGCCTCCCGTTCCAGCATTGCCTCACGGTACCgcgc                 >  5:233939/1‑149 (MQ=255)
                                                                                                                                  ttCCGGCAGCTGCATCGGCTGATGCAGACCGATGGAGGAGCGGCCCTGTGTCGCGAAAGGGCCGCTCGCACAAGGACCGCAAAGCGTCCCGGTTCGGTGGATCAGGCCGGCTCTGCCTCCCGTTCCAGCATTGCCTCACGGTACCgcgc                 >  2:189881/1‑149 (MQ=255)
                                                                                                                                          tcTGCATCAGCCGATGCAGACCGATGGAGGAGCGGCCCTGTGTCGCGAAAGGGCCGCTCGCACAAGGACCGCAAAGCGTCCCGGTTCGGTGGATCAGGCCGGCTCTGCCTCCCGTTCCAGCATTGCCTCACGGTACCGCGCCAGctccg         >  2:53195/2‑148 (MQ=255)
                                                                                                                                             gCATCGGCTGATGCAGACCGATGGAGGAGCGGCCCTGTGTCGCGAAAGGGCCGCTCGCACAAGGACCGCAAAGCGTCCCGGTTCGGTGGATCAGGCCGGCTCTGCCTCCCGTTCCAGCATTGCCTCACGGTACCgcgc                 <  3:129785/138‑1 (MQ=255)
                                                                                                                                             gCATCGGCTGATGCAGACCGATGGAGGAGCGGCCCTGTGTCGCGAAAGGGCCGCTCGCACAAGGACCGCAAAGCGTCCCGGTTCGGTGGATCAGGCCGGCTCTGCCTCCCGTTCCAGCATTGCCTCACGGTACCGCGCCAGCTCCTCGa      >  1:125677/1‑149 (MQ=255)
                                                                                                                                             gCATCGGCTGATGCAGACCGATGGAGGAGCGGCCCTGTGTCGCGAAAGGGCCGCTCGCACAAGGACCGCAAAGCGTCCCGGTTCGGTGGATCAGGCCGCCTCTGCCTCCCGTTCCAGCATTGCCTCACGGTACCgcgc                 >  4:129785/1‑138 (MQ=255)
                                                                                                                                             gCATCAGCCGATGCAGACCGATGGAGGAGCGGCCCTGTGTCGCGAAAGGGCCGCTCGCACAAGGACCGCAAAGCGTCCCGGTTCGGTGGATCAGGCCGGCTCTGCCTCCCGTTCCAGCATTGCCTCACGGTACCGCGCCAGc             <  7:232506/142‑1 (MQ=255)
                                                                                                                                             gCATCAGCCGATGCAGACCGATGGAGGAGCGGCCCTGTGTCGCGAAAGGGCCGCTCGCACAAGGACCGCAAAGCGTCCCGGTTCGGTGGATCAGGCCGGCTCTGCCTCCCGTTCCAGCATTGCCTCACGGTACCGCGCCAGc             >  8:232506/1‑142 (MQ=255)
                                                                                                                                              cATCGGCTGATGCAGACCGATGGAGGAGCGGCCCTGTGTCGCGAAAGGGCCGCTCGCACAAGGACCGCAAAGCGTCCCGGTTCGGTGGATCAGGCCGGCTCTGCCTCCCGTTCCAGCATTGCCTCACGGTACCGCGCCAGCTCCTCGAt     <  2:25943/149‑1 (MQ=255)
                                                                                                                                              cATCGGCTGATGCAGACCGATGGAGGAGCGGCCCTGTGTCGCGAAAGGGCCGCTCGCACAAGGACCGCAAAGCGTCCCGGTTCGGTGGATCAGGCCGGCTCTGCCTCCCGTTCCAGCATTGCCTCACGGTACCGCGCCAGCTCCTCGAt     <  5:236697/149‑1 (MQ=255)
                                                                                                                                                  ggCTGATGCAGACCGATGGAGGAGCGGCCCTGTGTCGCGAAAGGGCCGCTCGCACAAGGACCGCAAAGCGTCCCGGTTCGGTGGATCAGGCCGGCTCTGCCTCCCGTTCCAGCATTGCCTCACGGTACCGCGCCAGCTCCTCGATGGt  <  2:119610/148‑1 (MQ=255)
                                                                                                                                                  ggCTGATGCAGACCGATGGAGGAGCGGCCCTGTGTCGCGAAAGGGCCGCTCGCACAAGGACCGCAAAGCGTCCCGGTTCGGTGGATCAGGCCGGCTCTGCCTCCCGTTCCAGCATTGCCTCACGGTACCGCGCCAGCTCCTCGATGGt  >  1:179934/1‑148 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
CTCGACCAGTGCTTGCAGGTCTGCGAGGGATTGCTCGAAATCGAGGGAGGCTTTTTTGCGGGCCATGGCGACTGTCTCGGTGGCATTCAGAACGGCGCGACACTAGCAGAGCGGGGCGGGGGGAGCAAACTTCCGGCAGCTGCATCGGCTGATGCAGACCGATGGAGGAGCGGCCCTGTGTCGCGAAAGGGCCGCTCGCACAAGGACCGCAAAGCGTCCCGGTTCGGTGGATCAGGCCGGCTCTGCCTCCCGTTCCAGCATTGCCTCACGGTACCGCGCCAGCTCCTCGATGGT  >  NC_002947/615420‑615713

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑