Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 615,597 G→T 7.5% intergenic (‑112/+54) xseB ← / ← ribB exodeoxyribonuclease 7 small subunit/3,4‑dihydroxy‑2‑butanone‑4‑phosphate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947615,5970GT 7.5% 165.1 / 4.2 53intergenic (‑112/+54)xseB/ribBexodeoxyribonuclease 7 small subunit/3,4‑dihydroxy‑2‑butanone‑4‑phosphate synthase
Reads supporting (aligned to +/- strand):  ref base G (26/23);  new base T (2/2);  total (28/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.92e-01

ATCGAGGGAGGCTTTTTTGCGGGCCATGGCGACTGTCTCGGTGGCATTCAGAACGGCGCGACACTAGCAGAGCGGGGCGGGGGGAGCAAACTTCCGGCAGCTGCATCGGCTGATGCAGACCGATGGAGGAGCGGCCCTGTGTCGCGAAAGGGCCGCTCGCACAAGGACCGCAAAGCGTCCCGGTTCGGTGGATCAGGCCGGCTCTGCCTCCCGTTCCAGCATTGCCTCACGGTACCGCGCCAGCTCCTCGATGGTCAGCACCGGCAAATTGTACTGCCG  >  NC_002947/615459‑615737
                                                                                                                                          |                                                                                                                                            
aTCGAGGGAGGCTTTTTTGCGGGCCATGGCGACTGTCTCGGTGGCATTCAGAACGGCGCGACACTAGCAGAGCGGGGCGGGGGGAGTAAACTTCCGGCAGCTGCATCGGCTGATGCAGACCGATGGAGGAGCGGCCCTGTGTCGCGaaa                                                                                                                                    <  2:76599/149‑1 (MQ=255)
   gagggaggCTTTTTTGCGGGCCATGGCGACTGTCTCGGTGGCATTCAGAACGGCGCGACACTAGCAGAGCGGGGCGGGGGGAGCAAACTTCCGGCAGCTGCATCGGCTGATGCAGACCGATGGAGGAGCGGCCCTGTGTCGCGAAAgg                                                                                                                                  <  8:252600/148‑1 (MQ=255)
    agggaggCTTTTTTGCGGGCCATGGCGACTGTCTCGGTGGCATTCAGAACGGCGCGACACTAGCAGAGCGGGGCGGGGGGAGCAAACTTCCGGCAGATGCATCGGCTGATGCAGACCGATGGAGGAGCGGCCCTGTGTCGCGAAAGGGc                                                                                                                                >  1:25943/1‑149 (MQ=255)
          gCTTTTTTGCGGGCCATGGCGACTGTCTCGGTGGCATTCAGAACGGCGCGACACTAGCAGAGCGGGGCGGGGGGAGCAAACTTCCGGCAGCTGCATCGGCTGATGCAGACCGATGGAGGAGCGGCCCTGTGTCGCGAAAGGGCCGCTCg                                                                                                                          >  3:240365/1‑149 (MQ=255)
            ttttttGCGGGCCATGGCGACTGTCTCGGTGGCATTCAGAACGGCGCGACACTAGCAGAGCGGGGCGGGGGGAGCAAACTTCCGGCAGCTGCATCGGCTGATGCAGACCGATGGAGGAGCGGCCCTGTGTCGCGAAAGGGCCGCTCGca                                                                                                                        >  6:212940/1‑149 (MQ=255)
            ttttttGCGGGCCATGGCGACTGTCTCGGTGGCATTCAGAACGGCGCGACACTAGCAGAGCGGGGCGGGGGGAGCAAACTTCCGGCAGCTGCATCGGCTGATGCAGACCGATGGAGGAGCGGCCCTGTGTCGCGAAAGGGCCGCTCGca                                                                                                                        >  1:2914/1‑149 (MQ=255)
              ttttGCGGGCCATGGCGACTGTCTCGGTGGCATTCAGAACGGCGCGACACTAGCAGAGCGGGGCGGGGGGAGCAAACTTCCGGCAGCTGCATCGGCTGATGCAGACCGATGGAGGAGCGGCCCTGTGTCGCGAAAGGGCCGCTCGca                                                                                                                        <  8:1969/147‑1 (MQ=255)
                   cGGGCCATGGCGACTGTCTCGGTGGCATTCAGAACGGCGCGACACTAGCAGAGCGGGGCGGGGGGAGCAAACTTCCGGCAGCTGCATCGGCTGATGCAGACCGATGGAGGAGCGGCCCTGTGTCGCGAAAGGGCCGCTCGCACAAGGa                                                                                                                  <  5:212940/148‑1 (MQ=255)
                    gggCCATGGCGACTGTCTCGGTGGCATTCAGAACGGCGCGACACTAGCAGAGCGGGGCGGGGGGAGCAAACTTCCGGCAGCTGCATCGGCTGATGCAGACCGATGGAGGAGCGGCCCTGTGTCGCGAAAGGGCCGCTCGCACAAGGAcc                                                                                                                >  6:236697/1‑149 (MQ=255)
                       ccATGGCGACTGTCTCGGTGGCATTCAGAACGGCGCGACACTAGCAGAGCGGGGCGGGGGGAGCAAACTTCCGGCAGCTGCATCGGCTGATGCAGACCGATGGAGGAGCGGCCCTGTGTCGCGAAAGGGCCGCTCGCACAAGGACCGc                                                                                                              >  7:73923/1‑148 (MQ=255)
                         aTGGCGACTGTCTCGGTGGCATTCAGAACGGCGCGACACTAGCAGCGCGGGGCGGGGGGAGCAAACTTCCGGCAGCTGCATCGGCTGATGCAGACCGATGGAGGAGCGGCCCTGTGTCGCGAAAGGGCCGCTCGCACAAGGACCGCaaa                                                                                                           <  2:2914/149‑1 (MQ=255)
                                         tGGCATTCAGAACGGCGCGACACTAGCAGAGCGGGGCGGGGGGAGCAAACTTCCGGCAGCTGCATCGGCTGATGCAGACCGATGGAGGAGCGGCCCTTTCGCGACACAGGGCCGCt                                                                                                                            >  5:109382/1‑116 (MQ=38)
                                         tGGCATTCAGAACGGCGCGACACTAGCAGAGCGGGGCGGGGGGAGCAAACTTCCGGCAGCTGCATCGGCTGATGCAGACCGATGGAGGAGCGGCCCTTTCGCGACACAGGGCCGCt                                                                                                                            <  6:109382/116‑1 (MQ=38)
                                            cATTCAGAACGGCGCGACACTAGCAGAGCGGGGCGGGGGGAGCAAACTTCCGGCAGCTGCATCGGCTGATGCAGACCGATGGAGGAGCGGCCCTGTGTCGCGAAAGGGCCGCTCGCACAAGGACCGCAAAGCGTCCCGGTTCGGTGGAt                                                                                        >  1:119610/1‑149 (MQ=255)
                                                 aGAACGGCGCGACACTAGCAGAGCGGGGCGGGGGGAGCAAACTTCCGGCAGCTGCATCGGCTGATGCAGACCGATGGAGGAGCGGCCCTGTGTCGCGAAAGGGCCGCTCGCACAAGGACCGCAAAGCGTCCCGGTTCGGTGGATCAGGc                                                                                   >  1:81434/1‑149 (MQ=255)
                                                           gACACTAGCAGAGCGGGGCGGGGGGAGCAAACTTCCGGCAGCTGCATCGGCTGATGCAGACCGATGGAGGAGCGGCCCTGTGTCGCGAAAGGGCCGCTCGCACAAGGACCGCAAAGCGTCCCGGtt                                                                                                >  5:20393/1‑126 (MQ=255)
                                                           gACACTAGCAGAGCGGGGCGGGGGGAGCAAACTTCCGGCAGCTGCATCGGCTGATGCAGACCGATGGAGGAGCGGCCCTGTGTCGCGAAAGGGCCGCTCGCACAAGGACCGCAAAGCGTCCCGGtt                                                                                                <  6:20393/126‑1 (MQ=255)
                                                               cTAGCAGAGCGGGGCGGGGGGAGCAAACTTCCGGCAGCTGCATCGGCTGATGCAGACCGATGGAGGAGCGGCCCTGTGTCGCGAAAGGGCCGCTCGCACAAGGACCGCAAAGCGTCCCGGTTCGGTGGATCAGGCCGGCTCTGCCTccc                                                                     <  5:219197/149‑1 (MQ=255)
                                                                tAGCAGAGCGGGGCGGGGGGAGCAAACTTCCGACAGCTGCATCGGCTGATGCAGACCGATGGAGGAGCGGCCCTGTGTCGCGAAAGGGCCGCTCGCACAAGGACCGCAAAGCGTCCCGGTTCGGTGGATCAGGCCGGCTCTGCCTCCCg                                                                    >  8:83128/1‑149 (MQ=255)
                                                                  gCAGAGCGGGGCGGGGGGAGCAAACTTCCGGCAGCTGCATCGGCTGATGCAGACCGATGGAGGAGCGGCCCTGTGTCGCGAAAGGGCCGCTCGCACAAGGACCGCAAAGCGTCCCGGTTCGGTGGATCAGGCCGGCTCTGCCTCCCGtt                                                                  >  6:124939/1‑149 (MQ=255)
                                                                       gcggggctggGGGAGCAAACTTCCGGCAGCTGCATCGGCTGATGCAGACCGATGGAGGAGCGGCCCTGTGTCGCGAAAGGGCCGCTCGCACAAGGACCGCAAAGCGTCCCGGTTCGGTGGATCAGGCCGGCTCTGCCTCCCGTTCCAGc                                                             <  2:147341/149‑1 (MQ=255)
                                                                             cggggGGAGCAAACTTCCGGCAGCTGCATCGGCTGATGCAGACCGATGGAGGAGCGGCCCTGTGTCGCGAAAGGGCCGCTCGCACAAGGACCGCAAAGCGTCCCGGTTCGGTGGATCAGGCCGGCTCTGCCTCCCGTTCCAGCATTGcc                                                       <  5:124939/149‑1 (MQ=255)
                                                                                 gggAGCAAACTTCCGGCAGCTGCATCGGCTGATGCAGACCGATGGAGGAGCGGCCCTGTGTCGCGAAAGGGCCGCTCGCACAAGGACCGCAAAGCGTCCCGGTTCGGTGGATCAGGCCGGCTCTGCCTCCCGTTCCAGCATTGCCTCAc                                                   <  8:73923/149‑1 (MQ=255)
                                                                                 gggAGCAAACTTCCGACAGCTGCATCGGCTGATGCAGACCGATGGAGGAGCGGCCCTGTGTCGCGAAAGGGCCGCTCGCACAAGGACCGCAAAGCGTCCCGGTTCGGTGGATCAGGCCGGCTCTGCCTCCCGTTCCAGCATTGCCTCAc                                                   <  7:83128/149‑1 (MQ=255)
                                                                                     gCAAACTTCCGGCAGCTGCATCGGCTGATGCAGACCGATGGAGGAGCGGCCCTGTGTCGCGAAAGGGCCGCTCGCACAAGGACCGCAAAGCGTccc                                                                                                    >  3:54653/1‑96 (MQ=255)
                                                                                     gCAAACTTCCGGCAGCTGCATCGGCTGATGCAGACCGATGGAGGAGCGGCCCTGTGTCGCGAAAGGGCCGCTCGCACAAGGACCGCAAAGCGTccc                                                                                                    <  4:54653/96‑1 (MQ=255)
                                                                                       aaaCTTCCGGCAGCTGCATCGGCTGATGCAGACCGATGGAGGAGCGGCCCTGTGTCGCGAAAGGGCCGCTCGCACAAGGACCGCAAAGCGTCCCGGTTCGGTGGATCAGGCCGGCTCTGCCTCCCGTTCCAGCATTGCCTCACGGTAcc                                             >  5:232664/1‑149 (MQ=255)
                                                                                       aaaCTTCCGGCAGCTGCATCGGCTGATGCAGACCGATGGAGGAGCGGCCCTGTGTCGCGAAAGGGCCGCTCGCACAAGGACCGCAAAGCGTCCCGGTTCGGTGGATCAGGCCGGCTCTGCCTCCCGTTCCAGCATTGCCTCACGGTAcc                                             >  3:208675/1‑149 (MQ=255)
                                                                                           ttCCGGCAGCTGCATCGGCTGATGCAGACCGATGGAGGAGCGGCCCTGTGTCGCGAAAGGGCCGCTCGCACAAGGACCGCAAAGCGTCCCGGTTCGGTGGATCAGGCCGGCTCTGCCTCCCGTTCCAGCATTGCCTCACGGTACCgcgc                                         >  5:233939/1‑149 (MQ=255)
                                                                                           ttCCGGCAGCTGCATCGGCTGATGCAGACCGATGGAGGAGCGGCCCTGTGTCGCGAAAGGGCCGCTCGCACAAGGACCGCAAAGCGTCCCGGTTCGGTGGATCAGGCCGGCTCTGCCTCCCGTTCCAGCATTGCCTCACGGTACCgcgc                                         >  2:189881/1‑149 (MQ=255)
                                                                                                   tcTGCATCAGCCGATGCAGACCGATGGAGGAGCGGCCCTGTGTCGCGAAAGGGCCGCTCGCACAAGGACCGCAAAGCGTCCCGGTTCGGTGGATCAGGCCGGCTCTGCCTCCCGTTCCAGCATTGCCTCACGGTACCGCGCCAGctccg                                 >  2:53195/2‑148 (MQ=255)
                                                                                                      gCATCGGCTGATGCAGACCGATGGAGGAGCGGCCCTGTGTCGCGAAAGGGCCGCTCGCACAAGGACCGCAAAGCGTCCCGGTTCGGTGGATCAGGCCGGCTCTGCCTCCCGTTCCAGCATTGCCTCACGGTACCgcgc                                         <  3:129785/138‑1 (MQ=255)
                                                                                                      gCATCGGCTGATGCAGACCGATGGAGGAGCGGCCCTGTGTCGCGAAAGGGCCGCTCGCACAAGGACCGCAAAGCGTCCCGGTTCGGTGGATCAGGCCGGCTCTGCCTCCCGTTCCAGCATTGCCTCACGGTACCGCGCCAGCTCCTCGa                              >  1:125677/1‑149 (MQ=255)
                                                                                                      gCATCGGCTGATGCAGACCGATGGAGGAGCGGCCCTGTGTCGCGAAAGGGCCGCTCGCACAAGGACCGCAAAGCGTCCCGGTTCGGTGGATCAGGCCGCCTCTGCCTCCCGTTCCAGCATTGCCTCACGGTACCgcgc                                         >  4:129785/1‑138 (MQ=255)
                                                                                                      gCATCAGCCGATGCAGACCGATGGAGGAGCGGCCCTGTGTCGCGAAAGGGCCGCTCGCACAAGGACCGCAAAGCGTCCCGGTTCGGTGGATCAGGCCGGCTCTGCCTCCCGTTCCAGCATTGCCTCACGGTACCGCGCCAGc                                     >  8:232506/1‑142 (MQ=255)
                                                                                                      gCATCAGCCGATGCAGACCGATGGAGGAGCGGCCCTGTGTCGCGAAAGGGCCGCTCGCACAAGGACCGCAAAGCGTCCCGGTTCGGTGGATCAGGCCGGCTCTGCCTCCCGTTCCAGCATTGCCTCACGGTACCGCGCCAGc                                     <  7:232506/142‑1 (MQ=255)
                                                                                                       cATCGGCTGATGCAGACCGATGGAGGAGCGGCCCTGTGTCGCGAAAGGGCCGCTCGCACAAGGACCGCAAAGCGTCCCGGTTCGGTGGATCAGGCCGGCTCTGCCTCCCGTTCCAGCATTGCCTCACGGTACCGCGCCAGCTCCTCGAt                             <  5:236697/149‑1 (MQ=255)
                                                                                                       cATCGGCTGATGCAGACCGATGGAGGAGCGGCCCTGTGTCGCGAAAGGGCCGCTCGCACAAGGACCGCAAAGCGTCCCGGTTCGGTGGATCAGGCCGGCTCTGCCTCCCGTTCCAGCATTGCCTCACGGTACCGCGCCAGCTCCTCGAt                             <  2:25943/149‑1 (MQ=255)
                                                                                                           ggCTGATGCAGACCGATGGAGGAGCGGCCCTGTGTCGCGAAAGGGCCGCTCGCACAAGGACCGCAAAGCGTCCCGGTTCGGTGGATCAGGCCGGCTCTGCCTCCCGTTCCAGCATTGCCTCACGGTACCGCGCCAGCTCCTCGATGGt                          >  1:179934/1‑148 (MQ=255)
                                                                                                           ggCTGATGCAGACCGATGGAGGAGCGGCCCTGTGTCGCGAAAGGGCCGCTCGCACAAGGACCGCAAAGCGTCCCGGTTCGGTGGATCAGGCCGGCTCTGCCTCCCGTTCCAGCATTGCCTCACGGTACCGCGCCAGCTCCTCGATGGt                          <  2:119610/148‑1 (MQ=255)
                                                                                                            gCTGATGCAGACCGATGGAGGAGCGGCCCTGTGTCGCGAAAGGGCCGCTCGCACAAGGACCGCAAAGCGTCCCGGTTCGGTGGATCAGGCCGGCTCTGCCTCCCGTTCCAGCAtt                                                          <  6:161944/115‑1 (MQ=255)
                                                                                                            gCTGATGCAGACCGATGGAGGAGCGGCCCTGTGTCGCGAAAGGGCCGCTCGCACAAGGACCGCAAAGCGTCCCGGTTCGGTGGATCAGGCCGGCTCTGCCTCCCGTTCCAGCAtt                                                          >  5:161944/1‑115 (MQ=255)
                                                                                                                   cAGACCGATGGGGGAGCGGCCCTGTGTCGCGAAAGGGCCGCTCGCACAAGGACAGCAAAGCGTCCCGGTTCGGTGGATCAGGCCGGCTCTGCCTCCCGTTCCAGCATTGCCTCACGGTACCGCGCCAGCTCCTCGATGGTCAGCACCgg                 >  1:205265/1‑149 (MQ=255)
                                                                                                                    aGACCGATGGAGGAGCGGCCCTGTGTCGCGAAAGGGCCGCTCGCACAAGGACCGCAAAGCGTCCCGGTTCGGTGGATCAGGCCGGCTCTGCCTCCCGTTCCAGCATTGCCTCACGGTACCGCGCCAGCTCCTCGATGGTCAGCACCGGc                <  6:233939/149‑1 (MQ=255)
                                                                                                                        cGATGGAGGAGCGGCCCTGTGTCGCGAAAGGGCCGCTCGCACAAGGACCGCAAAGCGTCCCGGTTCGGTGGATCAGGCCGGCTCTGCCTCCCGTTCCAGCATTGCCTCACGGTACCGCGCCAGctcct                                 >  3:176670/1‑128 (MQ=255)
                                                                                                                        cGATGGAGGAGCGGCCCTGTGTCGCGAAAGGGCCGCTCGCACAAGGACCGCAAAGCGTCCCGGTTCGGTGGATCAGGCCGGCTCTGCCTCCCGTTCCAGCATTGCCTCACGGTACCGCGCCAGctcct                                 <  4:176670/128‑1 (MQ=255)
                                                                                                                         gATGGAGGAGCGGCCCTGTGTCGCGAAAGGGCCGCTCGCACAAGGACCGCAAAGCGTCCCGGTTCGGTGGATCAGGCCGGCTCTGCCTCCCGTTCCAGCATTGGCTCACGGTACCGCGCCAGCTCCTCGATGGTCAGCACCGGCAAAtt           <  1:189881/149‑1 (MQ=255)
                                                                                                                         gATGGAGGAGCGGCCCTGTGTCGCGAAAGGGCCGCTCGCACAAGGACCGCAAAGCGTCCCGGTTCGGTGGATCAGGCCGGCTCTGCCTCCCGTTCCAGCATTGCCTCACGGTACCGCGCCAGCTCCTCGATGGTCAGCACCGGCAAAtt           <  2:125677/149‑1 (MQ=255)
                                                                                                                             gaggagCGGCCCTGTGTCGCGAAAGGGCCGCTCGCACAAGGACCGCAAAGCGTCCCGGTTCGGTGGATCAGGCCGGCTCTGCCTCCCGTTCCAGCATTGCCTCACGGTACCGCGCCAGCTCCTCGATGGTCAGCACCGGCAAATTGTa        <  1:53195/148‑1 (MQ=255)
                                                                                                                                gagCGGCCCTTTCGCGACACAGGGCCGCTCGCACAAGGACCGCAAAGCGTCCCGGTTCGGTGGATCAGGCCGGCTCTGCCTCCCGTTCCAGCATTGCCTCAc                                                   >  3:187472/1‑102 (MQ=38)
                                                                                                                                gagCGGCCCTTTCGCGACACAGGGCCGCTCGCACAAGGACCGCAAAGCGTCCCGGTTCGGTGGATCAGGCCGGCTCTGCCTCCCGTTCCAGCATTGCCTCAc                                                   <  4:187472/102‑1 (MQ=38)
                                                                                                                                gagCGGCCCTGTGTCGCGAAAGGGCCGCTCGCACAAGGACCGCAAAGCGTCCCGGTTCGGTGGATCAGGCCGGCTCTGCCTCCCGTTCCAGCATTGCCTCACGGTACCGCGCCAGCTCCTCGATGGTCAGCACCGGCAAATTGTACTGc    >  3:203044/1‑149 (MQ=255)
                                                                                                                                  gCGGCCCTGTGTCGCGAAAGGGCCGCTCGCACAAGGACCGCAAAGCGTCCCGGTTCGGTGGATCAGGCCGGCTCTGCCTCCAGTTCCAGCATTGCCTCACGGTACCGCGCCAGCTCCTCGATGGTCAGCACCGGCAAATTGTACTGCCg  >  8:53825/1‑149 (MQ=255)
                                                                                                                                          |                                                                                                                                            
ATCGAGGGAGGCTTTTTTGCGGGCCATGGCGACTGTCTCGGTGGCATTCAGAACGGCGCGACACTAGCAGAGCGGGGCGGGGGGAGCAAACTTCCGGCAGCTGCATCGGCTGATGCAGACCGATGGAGGAGCGGCCCTGTGTCGCGAAAGGGCCGCTCGCACAAGGACCGCAAAGCGTCCCGGTTCGGTGGATCAGGCCGGCTCTGCCTCCCGTTCCAGCATTGCCTCACGGTACCGCGCCAGCTCCTCGATGGTCAGCACCGGCAAATTGTACTGCCG  >  NC_002947/615459‑615737

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑