Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 625,062 C→T 8.8% intergenic (‑156/+27) PP_0537 ← / ← ppa transcriptional regulator/inorganic pyrophosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947625,0620CT 8.8% 157.3 / 5.1 57intergenic (‑156/+27)PP_0537/ppatranscriptional regulator/inorganic pyrophosphatase
Reads supporting (aligned to +/- strand):  ref base C (28/24);  new base T (3/2);  total (31/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.85e-01

TGTAGCGGCGGGTTGCCAGTAAGGAAATGAACCGGGTGTTGACGGGATTTGTACGAAAAGAGGCTGTTTGTAGGATGCTGGTGTAGGACTGTGTAGGATTTGTTCCCAGCAGTGAGCGCGTTCCATTCAGGCATGAAAAACCCCGCCAAAGCGGGGTTTCCCTGCAGTCGAAATCAGCCCTTGTAGGCAGCGACCGATTTGGTGATCGCGGCACGGGCGGCGTCGGCGCCTTCCCAGCCTTCGATCTTGACCCACTTGCCCTTCTCGAGATCTTTGTAGTTGGCAAAGAAGTGC  >  NC_002947/624916‑625209
                                                                                                                                                  |                                                                                                                                                   
tgtAGCGGCGGGTTGCCAGTAAGGAAATGAACCGGGTGTTGACGGGATTTGTACGAAAAGAGGCTGTTTGTAGGATGCTGGTGTAGGACTGTGTAGGATTTGTTCCCAGCAGTGAGCGCGTTCCATTCAGGCATGAAAAACCCCGCCa                                                                                                                                                    >  3:48549/1‑148 (MQ=255)
      ggcggGTTGCCAGTAAGGAAATGAACCGGGTGTTGACGGGATTTGTACGAAAAGAGGCTGTTTGTAGGATGCTGGTGTAGGACTGTGTAGGATTTGTTCCCAGCAGTGAGCGCGTTCCATTCAGGCATGAAAAACCCCGCCAAAGCggg                                                                                                                                             <  4:129593/149‑1 (MQ=255)
         gggTTGCCAGTAAGGAAATGAACCGGGTGTTGACGGGATTTGTACGAAAAGAGGCTGTTTGTAGGATGCTGGTGTAGGACTGTGTAGGATTTGTTCCCAGCAGTGAGCGCGTTCCATTCAGGCATGAAAAACCCCGCCAAAGCGGGGtt                                                                                                                                          >  5:82733/1‑149 (MQ=255)
          ggTTGCCAGTAAGGAAATGAACCGGGTGTTGACGGGATTTGTACGAAAAGAGGCTGTTTGTAGGATGCTGGTGTAGGACTGTGTAGGATTTGTTCCCAGCAGTGAGCGCGTTCCATTCAGGCATGAAAAACCCCGCCAAAGCGGGGttt                                                                                                                                         <  5:161662/149‑1 (MQ=255)
                      ggAAATGAACCGGGTGTTGACGGGATTTGTACGAAAAGAGGCTGTTTGTAGGATGCTGGTGTAGGACTGTGTAGGATTTGTTCCCAGCAGTGAGCGCGTTCCATTCAGGCATGAAAAACCCCACCAAAGCGGGGTTTCCCTGCAGTCg                                                                                                                              >  2:176140/1‑148 (MQ=255)
                        aaaTGAACCGGGTGTTGACGGGATTTGTACGAAAAGAGGCTGTTTGTAGGATGCTGGTGTAGGACTGTGTAGGATTTGTTCCCAGCAGTGAGCGCGTTCCATTCAGGCATGAAAAACCCCGCCAAAGCGGGGTTTCCCTGCAGTCGaaa                                                                                                                           >  7:112019/1‑149 (MQ=255)
                         taTGAACCGGGTGTTGAAGGGATTTGTACGAAAAGAGGCTGTTTGTAGGATGCTGGTGTAGGACTGTGTAGGATTTGTTCCCAGCAGTGAGCGCGTTCCATTCAGGCATGAAAAACCCCGCCAAAGCGGGGTTTCCCTGCAGTCGAAAt                                                                                                                          <  5:208091/148‑1 (MQ=255)
                            gAACCGGGTGTTGACGGGATTTGTACGAAAAGAGGCTGTTTGTAGGATGCTGGTGTAGGACTGTGTAGGATTTGTTCCCAGCAGTGAGCGCGTTCCATTCAGGCATGAAAAACCCCGCCAAAGCGGGGTTTCCCTGCAGTCGa                                                                                                                             >  4:199094/1‑143 (MQ=255)
                            gAACCGGGTGTTGACGGGATTTGTACGAAAAGAGGCTGTTTGTAGGATGCTGGTGTAGGACTGTGTAGGATTTGTTCCCAGCAGTGAGCGCGTTCCATTCAGGCATGAAAAACCCCGCCAAAGCGGGGTTTCCCTGCAGTCGa                                                                                                                             <  3:199094/143‑1 (MQ=255)
                               ccGGGTGTTGACGGGATTTGTACGAAAAGAGGCTGTTTGTAGGATGCTGGTGTAGGACTGTGTAGGATTTGTTCCCAGCAGTGAGCGCGTTCCATTCAGGCATGAAAAACCCCGCCAAAGCGGGGTTTCCCTGCAGTCGAAATCAGccc                                                                                                                    <  8:112019/149‑1 (MQ=255)
                               ccGGGTGTTGACGGGATTTGTACGAAAAGAGGCTGTTTGTAGGATGCTGGTGTAGGACTGTGTAGGATTTGTTCACAGCAGTGAGCGCGTTCCATTCAGGCATGAAAAACCCCGCCAAAGCGGGGTTTCCCTGCAGTCGAAATCAGccc                                                                                                                    >  3:80242/1‑149 (MQ=255)
                               ccGGGTGTTGACGGGATTTGTACGAAAAGAGGCTGTTTGTAGGATGCTGGTGTAGGACTGTGTAGGATGTGTTCCCAGCAGTGAGCGCGTTCCATTCAGGCATGAAAAACCCCGCCAAAGCGGGGTTTCCCTGCAGTCGAAATCAGccc                                                                                                                    <  7:174432/149‑1 (MQ=255)
                                    tgtTGACGGGATTTGTACGAAAAGAGGCTGTTTGTAGGATGCTGGTGTAGGACTGTGTAGGATTTGTTCCCAGCAGTGAGCGCGTTCCATTCAGGCATGAAAAACCCCGCCAAAGCGGGGTTTCCCTGCAGTCGAAATCAGCCCTTGTa                                                                                                               <  2:254769/149‑1 (MQ=255)
                                    tgtTGACGGGATTTGTACGAAAAGAGGCTGTTTGTAGGATGCTGGTGTAGGACTGTGTAGGATTTGTTCCCAGCAGTGAGCGCGTTCCATTCAGGCATGAAAAACCCCGCCAAAGCGGGGTTTCCCTGCAGTCGAAATCAGCCCTTGTa                                                                                                               <  4:48549/149‑1 (MQ=255)
                                      ttGACGGGATTTGTACGAAAAGAGGCTGTTTGTAGGATGCTGGTGTAGGACTGTGTAGGATTTGTTCCCAGCAGTGAGCGCGTTCCATTCAGGCATGAAAAACCCCGCCAAAGCGGGGTTTCCCTGCAGTCGAAATCAGCCCTTGTAgg                                                                                                             >  7:214346/1‑149 (MQ=255)
                                        gACGGGATTTGTACGAAAAGAGGCTGTTTGTAGGATGCTGGTGTAGGACTGTGTAGGATTTGTTCCCAGCAGTGAGCGCGTTCCATTCAGGCATGAAAAACCCCGCCAAAGCGGGGTTTCCCTGCAGTCGAAATCAGCCCTTGTAGGc                                                                                                            <  4:80242/148‑1 (MQ=255)
                                        gACGGGATTTGTACGAAAAGAGGCTGTTTGTAGGATGCTGGTGTAGAACTGTGTAGGATTTGTTCCCAGCAGTGAGCGCGTTCCATTCAGGCATGAAAAACCCCGCCAAAGCGGGGTTTCCCTGCAGTCGAAATCAGCCCTTGTAgg                                                                                                             >  1:59218/1‑147 (MQ=255)
                                         aCGGGATTTGTACGAAAAGAGGCTGTTTGTAGGATGCTGGTGTAGGACTGTGTAGGATTTGTTCCCAGCAGTGAGCGCGTTCCATTCAGGCATGAAAAACCCCGCCAAAGCGGGGTTTCCCTGCAGTCGAAATCAGCCCTTGTAGGCa                                                                                                           <  2:59218/148‑1 (MQ=255)
                                         aCGGGATTTGTACGAAAAGAGGCTGTTTGTAGGATGCTGGTGTAGGACTGTGTAGGATTTGTTCCCAGCAGTGAGCGCGTTCCATTCAGGCATGAAAAACCCCGCCAAAGCGGGGTTTCCCTGCAGTCGAAATCAGCCCTTGTAGGCAg                                                                                                          >  1:253753/1‑149 (MQ=255)
                                             gATTTGTACGAAAAGAGGCTGTTTGTAGGATGCTGGTGTAGGACTGTGTAGGATTTGTTCCCAGCAGTGAGCGCGTTCCATTCAGGCATGAAAAACCCCGCCAAAGCGGGGTTTCCCTGCAGTCGAAATCAGCCCTTGTAGGCAGCGAc                                                                                                      <  6:82733/149‑1 (MQ=255)
                                             gATTTGTACGAAAAGAGGCTGTTTGTAGGATGCTGGTGTAGGACTGTGTAGGATTTGTTCCCAGCAGTGAGCGCGTTCCATTCAGGCATGAAAAACCCCGCCAAAGCGGGGTTTCCCTGCAGTCGAAATCAGCCCTTGTAGGCAGCGAc                                                                                                      >  2:180073/1‑149 (MQ=255)
                                                   tACGAAAAGAGGCTGTTTGTAGGATGCTGGTGTAGGACTGTGTAGGATTTGTTCCCAGCAGTGAGCGCGTTCCATTCAGGCATGAAAAACCCCGCTTTGGCGGGGtttttcat                                                                                                                                    >  6:80775/1‑108 (MQ=255)
                                                   tACGAAAAGAGGCTGTTTGTAGGATGCTGGTGTAGGACTGTGTAGGATTTGTTCCCAGCAGTGAGCGCGTTCCATTCAGGCATGAAAAACCCCGCTTTGGCGGGGtttttcat                                                                                                                                    <  5:80775/113‑6 (MQ=255)
                                                    aCGAAAAGAGGCTGTTTGTAGGATGCTGGTGTAGGACTGTGTAGGATTTGTTCCCAGCAGTGAGCGCGTTCCATTCAGGCATGAAAAACCCCGCCAAAGCGGGGTTTCCCTGCAGTCGAAATCAGCCCTTGTAGGCAGCGACCGATTTg                                                                                               >  7:2730/1‑149 (MQ=255)
                                                     cGAAAAGATGCTGTTTGTAGGATGCTGGTGTAGGACTGTGTAGGATTTGTTCCCAGCAGTGAGCGCGTTCCATTCAGGCATGAAAAACCCCGCCAAAGCGGGGTTTCCCTGCAGTCGAAATCAGCCCTTGTAGGCAGCGACCGATTTgg                                                                                              <  6:16837/149‑1 (MQ=255)
                                                      gAAAAGAGGCTGTTTGTAGGATGCTGGTGTAGGACTGTGTAGGATTTGTTCCCAGCAGTGAGCGCGTTCCATTCAGGCATGAAAAACCCCGCCAAAGCGGGGTTTCCCTGCAGTCGAAATCAGCCCTTGTAGGCAGCGACCGATTTGGt                                                                                             <  8:2730/149‑1 (MQ=255)
                                                       aaaaGAGGCTGTTTGTAGGATGCTGGTGTAGGACTGTGTAGGATTTGTTCCCCGCAGTGAGCGCGTTCGATTCAGGCATGAAAAACCCCGCCAAAGCGGGGTGTCCCTGCAGTCGAAAACAGCCCTTGGAGGCAGCGACAGCTgtggtg                                                                                            >  8:196799/1‑149 (MQ=255)
                                                                     gTAGGATGCTGGTGTAGGACTGTGTAGGATTTGTTCCCAGCAGTGAGCGCGTTCCATTTAGGCATGAAAAACCCCGCCAAAGCGGGGTTTCCCTGCAGTCGAAATCAGCCCTTGTAGGCAGCGACCGATTTGGTGATCGCGGCACGggc                                                                              >  4:169384/1‑149 (MQ=255)
                                                                     gTAGGATGCTGGTGTAGGACTGTGTAGGATTTGTTCCCAGCAGTGAGCGCGTTCCATTCAGGCATGAAAAACCCCGCCAAAGCGGGGTTTCCCTGCAGTCGAAATCAGCCCTTGTAGGCAGCGACCGATTTGGTGATCGCGGCACGggc                                                                              >  7:21731/1‑149 (MQ=255)
                                                                              tGGTGTAGGACTGTGTAGGATTTGTTCCCAGCAGTGAGCGCGTTCCATTCAGGCATGAAAAACCCCGCCAAAGCGGGGTTTCCCTGCAGTCGAAATCAGCCCTTGTAGGCAGCGACCGATTTGGTGATCGCGGCACGGGCGGCGTCGgc                                                                     >  4:160018/1‑149 (MQ=255)
                                                                                gtgtAGGACTGTGTAGGATTTGTTCCCAGCAGTGAGCGCGTTCCATTCAGGCATGAAAAACCCCGCCAAAGCGGGGTTTCCCTGCAGTCGAAATCAGCCCTTGTAGGCAGCGACCGATTTGGTGATCGCGGCACGGGCGGCGTCGgcgc                                                                   >  3:180909/1‑149 (MQ=255)
                                                                                      gACTGTGTAGGATTTGTTCCCAGCAGTGAGCGCGTTCCATTCAGGCATGAAAAACCCCGCCAAAGCGGGGTTTCCCTGCAGTCGAAATCAGCCCTTGTAGGCAGCGACCGATTTGGTGATCGCGGCACGGGCGGCGTCGGCGCCTTccc                                                             <  1:180073/149‑1 (MQ=255)
                                                                                         tgtgTAGGATTTGTTCCCAGCAGTGAGCGCGTTCCATTCAGGCATGAAAAACCCCGCCAAAGCGGGGTTTCCCTGCAGTCGAAATCAGCCCTTGTAGGCAGCGACCGATTTGGTGATCGCGGCACGGGCGGCGTCGGCGCCTTCCCAGc                                                          >  8:124004/1‑149 (MQ=255)
                                                                                             tAGGATTTGTTCCCAGCAGTGAGCGCGTTCCATTCAGGCATGAAAAACCCCGCCAAAGCGGGGTTTCCCTGCAGTCGAAATCAGCCCTTGTAGGCAGCGACCGATTTGGTGATCGCGGCACGGGCGGCGTCGGCGCCTTCCCAGCCTTc                                                      >  7:137688/1‑149 (MQ=255)
                                                                                              aGGATTTGTTCCCAGCAGTGAGCGCGTTCCATTCAGGCATGAAAAACCCCGCCAAAGCGGGGTTTCCCTGCAGTCGAAATCAGCCCTTGTAGGCAGCGACCGATTTGGTGATCGCGGCACGGGCGGCGTCGGCGCCTTCCCAGCCTTc                                                      >  2:197349/1‑148 (MQ=255)
                                                                                               ggATTTGTTCCCAGCAGTGAGCGCGTTCCATTCAGGCATGAAAAACCCCGCCAAAGCGGGGTTTCCCTGCAGTCGAAATCAGCCCTTGTAGGCAGCGACCGATTTGGTGATCGCGGCACGGGCGGCGTCGGCGCCTTCCCAGCCTTCGa                                                    <  4:180909/149‑1 (MQ=255)
                                                                                               ggATTTGTTCCCAGCAGTGAGCGCGTTCCATTCAGGCATGAAAAACCCCACCAAAGCGGGGTTTCCCTGCAGTCGAAATCAGCCCTTGTAGGCAGCGACCGATTTGGTGATCTCGGCACGGGCGGCGTCGGCGCCTTCCCAGCCTTCg                                                     <  1:176140/148‑1 (MQ=255)
                                                                                                gATTTGTTCCCAGCAGTGAGCGCGTTCCATTCAGGCATGAAAAACCCCGCCAAAGCGGGGTTTCCCTGCAGTCGAAATCAGCCCTTGTAGGCAGCGACCGATTTGGTGATCGCGGCACGGGCGGCGTCGGCGCCTTCCCAGCCTTCGa                                                    >  3:83858/1‑148 (MQ=255)
                                                                                                 aTTTGTTCCCAGCAGTGAGCGCGTTCCATTCAGGCATGAAAAACCCCGCCAAAGCGGGGTTTCCCTGCAGTCGAAATCAGCCCTTGTAGGCAGCGACCGATTTGGTGATCGCGGCACGGGCGGCGTCGGCGCCTTCCCAGCCTTCGATc                                                  >  5:67285/1‑149 (MQ=255)
                                                                                                 aTTTGTTCCCAGCAGTGAGCGCGTTCCATTCAGGCATGAAAAACCCCGCCAAAGCGGGGTTTCCCTGCAGTCGAAATCAGCCCTTGTAGGCAGCGACCGATTTGGTGATCGCGGCACGGGCGGCGTCGGCGCCTTCCCAGCCTTCGATc                                                  <  3:160018/149‑1 (MQ=255)
                                                                                                 aTTTGTTCCCAGCAGTGAGCGCGTTCCATTCAGGCATGAAAAACCCCGCCAAAGCGGGGTTTCCCTGCAGTCGAAATCAGCCCTTGTAGGCAGCGACCGATTTGGTGATCGCGGCACGGGCGGCGTCGGCGCCTTCCCAGCCTTCGATc                                                  >  1:39485/1‑149 (MQ=255)
                                                                                                            gcagTGAGCGCGTTCCATTCAGGCATGAAAAACCCCGCCAAAGCGTGGTTTCCCTGCAGTCGAAATCAGCCCTTGTAGGCAGCGACCGATTTGGTGATCGCGGCACGGGCGGCGTCGGCGCCTTCCCAGCCTTCGATCTTGACCCAc                                         >  8:139144/1‑147 (MQ=255)
                                                                                                                  aGCGCGTTCCATTCAGGCATGAAAAACCCCGCCAAAGCGGGGTTTCCCTGCAGTCGAAATCAGCCCTTGTAGGCAGCGACCGATTTGGTGATCGCGGCACGGGCGGCGTCGGCGCCTTCCCAGCCTTCGATCTTGACCCACTTGCCCtt                                 >  4:153247/1‑149 (MQ=255)
                                                                                                                   gcgcGTTCCATTCAGGCATGAAAAACCCCGCCAAAGCGGGGTTTCCCTGCAGTCGAAATCAGCCCTTGTAGGCAGCGACCGATTTGGTGATCGCGGCACGGGCGGCGTCGGCGCCTTCCCAGCCTTCGATCTTGACCCACtt                                       <  5:52241/142‑1 (MQ=255)
                                                                                                                   gcgcGTTCCATTCAGGCATGAAAAACCCCGCCAAAGCGGGGTTTCCCTGCAGTCGAAATCAGCCCTTGTAGGCAGCGACCGATTTGGTGATCGCGGCACGGGCGGCGTCGGCGCCTTCCCAGCCTTCGATCTTGACCCACtt                                       >  6:52241/1‑142 (MQ=255)
                                                                                                                     gcgTTCCATTCAGGCATGAAAAACCCCGCCAAAGCGGGGTTTCCCTGCAGTCGAAATCAGCCCTTGTAGGCAGCGACCGATTTGGTGATCGCGGCACGGGCGGCGTCGGCGCCTTCCCAGCCTTCGATCTTGACCCACTTGCCCTtctc                              <  2:253753/149‑1 (MQ=255)
                                                                                                                            aTTCAGGCATGAAAAACCCCGCCAAAGCGGGGTTTCCCTGCAGTCGAAATCAGCCCTTGTAGGCAGCGACCGATTTGGTGATCGCGGCACGGGCGGCGTCGGCGCCTTCCCAGCCTTCGATCTTGACCCACTTGCCCTTCTCGAGATCt                       <  1:197349/149‑1 (MQ=255)
                                                                                                                            aTTCAGGCATGAAAAACCCCGCCAAAGCGGGGTTTCCCTGCAGTCGAAATCAGCCCTTGTAGGCAGCGACCGATTTGGTGATCGCGGCACGGGCGGCGTCGGCGCCTTCCCAGCCTTCGATCTTGACCCACTTGCCCTTCTCGAGATCt                       <  7:139144/149‑1 (MQ=255)
                                                                                                                                 ggCATGAAAAACCCCGCCAAAGCGGGGTTTCCCTGCAGTCGAAATCAGCCCTTGTAGGCAGCGACCGATTTGGTGATCGCGGCACGGGCGGCGTCGGCGCCTTCCCAGCCTTCGATCTTGACCCACTTGCCCTTCTCGAGATCTTTgnn                  <  2:132852/149‑3 (MQ=255)
                                                                                                                                     tGAAAAACCCCGCCAAAGCGGGGTTTCCCTGCAGTCGAAATCAGCCCTTGTAGGCAGCGACCGATTTGGTGATCGCGGCACGGGCGGCGTCGGCGCCTTCCCAGCCTTCGATCTTGACCCACTTGCCCTTCTCGAGATCTTTGTAGTTg              <  6:67285/149‑1 (MQ=255)
                                                                                                                                      gAAAAACCCCGCCAAAGCGGGGTTTCCCTGCAGTCGAAATCAGCCCTTGTAGGCAGCGACCGATTTGGTGATCGCGGCACGGGCGGCGTCGGCGCCTTCCCAGCCTTCGATCTTGACCCACTTGCCCTTCTCGAGATCTTTGTAGTTgg             >  3:209444/1‑149 (MQ=255)
                                                                                                                                       ggaaaCCCCGCTTTGGCGGGGTTTTCCTGCAGTCGAAATCAGCCCTTGTAGGCAGCGACCGATTTGGTGATCGCGGCACGGGCGGCGTCGGCGCCTTCCCAGCCTTCGATCTTGACCCACTTGCCCTTCTCgag                           >  6:156155/3‑134 (MQ=255)
                                                                                                                                       ggaaaCCCCGCTTTGGCGGGGTTTTCCTGCAGTCGAAATCAGCCCTTGTAGGCAGCGACCGATTTGGTGATCGCGGCACGGGCGGCGTCGGCGCCTTCCCAGCCTTCGATCTTGACCCACTTGCCCTTCTCgag                           <  5:156155/132‑1 (MQ=255)
                                                                                                                                       ggaaaCCCCGCTTTGGCGGGGTTTCCCTGCAGTCGAAATCAGCCCTTGTAGGCAGCGACCGATTTGGTGATCGCGGCACGGGCGGCGTCGGCGCCTTCCCAGCCTTCGATCTTGACCCACTTGCCCTTCTCGAGATCTTTGTAGTTgg             >  5:12317/3‑148 (MQ=255)
                                                                                                                                        aaaaCCCCGCCAAAGCGGGGTTTCCCTGCAGTCGAAATCAGCCCTTGTAGGCAGCGACCGATTTGGTGATCGCGGCACGGGCGGCGTCGGCGCCTTCCCAGCCTTCGATCTTGACCCACTTGCCCTTCTCga                            <  3:141153/132‑1 (MQ=255)
                                                                                                                                        aaaaCCCCGCCAAAGCGGGGTTTCCCTGCAGTCGAAATCAGCCCTTGTAGGCAGCGACCGATTTGGTGATCGCGGCACGGGCGGCGTCGGCGCCTTCCCAGCCTTCGATCTTGACCCACTTGCCCTTCTCga                            >  4:141153/1‑132 (MQ=255)
                                                                                                                                              ccGCCAAAGCGGGGTTTCCCTGCAGTCGAAATCAGCCCTTGTAGGCAGCGACCGATTTGGTGATCGCGGCACGGGCGGCGTCGGCGCCTTCCCAGCCTTCGATCTTGACCCACTTGCCCTTCTCGAGATCTTTGTAGTTGGCAaagaag     >  4:165705/1‑149 (MQ=255)
                                                                                                                                                 ccAAAGCGGGGTTTCCCTGCAGTCGAAATCAGCCCTTGTAGGCAGCGACCGATTTg                                                                                               <  1:43499/56‑1 (MQ=255)
                                                                                                                                                 ccAAAGCGGGGTTTCCCTGCAGTCGAAATCAGCCCTTGTAGGCAGCGACCGATTTg                                                                                               >  2:43499/1‑56 (MQ=255)
                                                                                                                                                 ccAAAGCGGGGTTTCCCTGCAGTCGAAATCAGCCCTTGTAGGCAGCGACCGATTTGGTGATCGCGGCACGGGCGGCGTCGGCGCCTTCCCAGCCTTCGATCTTGACCCACTTGCCCTTCTCGAGATCTTTGTAGTTGGCAAAGAAGTGc  <  4:252124/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
TGTAGCGGCGGGTTGCCAGTAAGGAAATGAACCGGGTGTTGACGGGATTTGTACGAAAAGAGGCTGTTTGTAGGATGCTGGTGTAGGACTGTGTAGGATTTGTTCCCAGCAGTGAGCGCGTTCCATTCAGGCATGAAAAACCCCGCCAAAGCGGGGTTTCCCTGCAGTCGAAATCAGCCCTTGTAGGCAGCGACCGATTTGGTGATCGCGGCACGGGCGGCGTCGGCGCCTTCCCAGCCTTCGATCTTGACCCACTTGCCCTTCTCGAGATCTTTGTAGTTGGCAAAGAAGTGC  >  NC_002947/624916‑625209

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑