| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 650,846 | T→A | 14.7% | intergenic (‑135/+45) | aroQ‑I ← / ← dsbD‑I | type II 3‑dehydroquinate dehydratase/thiol/disulfide interchange protein 1 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 650,846 | 0 | T | A | 14.7% | 77.4 / 7.1 | 34 | intergenic (‑135/+45) | aroQ‑I/dsbD‑I | type II 3‑dehydroquinate dehydratase/thiol/disulfide interchange protein 1 |
| Reads supporting (aligned to +/- strand): ref base T (19/10); new base A (3/2); total (22/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.08e-01 | |||||||||||
GATTCCTTGGATTTGTAGGGCAATTCGACACAGCGCGGCGAGTGTGCCGTAAAGCAACGGCAAGTGTCCAGTTCCCGGCAATAGCCAGCACGATGTCCGAGGTTCGCGCGAAGTATGTGACCGGATATTTAAATCCGGTCACTCATTTCAGGATTTTTCCGAGCTCGCGGGAAGTTATAGACCGACTTTGCCGCGCACGCGCGTCAGAATCTGCGCGAATTCACCGGCATTTATCTCGCCAATCACCCGATCAGCGGTAATTTCGCTGCCGTTCG > NC_002947/650717‑650991 | gATTCCTTGGATTTGTAGGGCAATTCGACACAGCGCGGCGAGTGTGCCGTAAAGCAACGGCAAGTGTCCAGTTCCCGGCAATAGCCAGCACGATGTCCGAGGTTCGCGCGAAGTATGTGACCGGATATTTAAATCCGGTCACTCAttt > 7:173528/1‑148 (MQ=255) ttGGATTTGTAGGGCAATTCGACACAGCGCGGCGAGTGTGCCGTAAAGCAACGGCAAGTGTCCAGTTCCCGGCAATAGCCAGCACGATGTCCGAGGTTCGCGCGAAGTATGTGACCGGATTTAAATATCCGGTCAca < 7:72965/137‑2 (MQ=255) ttGGATTTGTAGGGCAATTCGACACAGCGCGGCGAGTGTGCCGTAAAGCAACGGCAAGTGTCCAGTTCCCGGCAATAGCCAGCACGATGTCCGAGGTTCGCGCGAAGTATGTGACCGGATTTAAATATCCGGTCAca > 8:72965/1‑136 (MQ=255) ttGGATTTGTAGGGCAATTCGACACAGCGCGGCGAGTGTGCCGTAAAGCAACGGCAAGTGTCCAGTTCCCGGCAATAGCCAGCACGATGTCCGAGGTTCGCGCGAAGTATGTGACCGGATATTTAAATCCGGTCACTCATTTCAGGAtt > 3:5607/1‑149 (MQ=255) aTTTGTAGGGCAATTCGACACAGCGCGGCGAGTGTGCCGTAAAGCAACGGCAAGTGTCCAGTTCCCGGCAATAGCCAGCACGATGTCCGAGGTTCGCGCGAAGTATGTGACCGGATTTAAATATCCGGTc > 8:8671/1‑130 (MQ=255) aTTTGTAGGGCAATTCGACACAGCGCGGCGAGTGTGCCGTAAAGCAACGGCAAGTGTCCAGTTCCCGGCAATAGCCAGCACGATGTCCGAGGTTCGCGCGAAGTATGTGACCGGATTTAAATATCCGGTc < 7:8671/130‑1 (MQ=255) gTAGGGCAATTCGACACAGCGCGGCGAGTGTGCCGTAAAGCAACGGCAAGTGTCCAGTTCCCGGCAATAGCCAGCACGATGTCCGAGGTTCGCGCGAAGTATGTGACCGGATATTTAAATCCGGTCACTCATTTCAGGATTTTTCCGa < 8:173528/148‑1 (MQ=255) ggCAATTCGACACAGCGCGGCGAGTGTGCCGTAAAGCAACGGCAAGTGTCCAGTTCCCGGCAATAGCCAGCACGATGTCCGAGGTTCGCGCGAAGTATGTGACCGGATATTTAAATCCGGTCACTCATTTCAGGATTTTTCCGAGCTcg > 1:126471/1‑149 (MQ=255) ggCAATTCGACACAGCGCGGCGAGTGTGCCGTAAAGCAACGGCAAGTGTCCAGTTCCCGGCAATAGCCAGCACGATGTCCGAGGTTCGCGCGAAGTATGTGACCGGATATTTAAATCCGGTCACACATTTCAGGATTTTTCCGAGCTcg > 3:130466/1‑149 (MQ=255) acaGCGCGGGGAGTGTGCCGTAAAGCAACGGAAAGTGTCCAGTTCCCGGCAATAGGCAGCACGATGTCCGAGGTTCGCGCTAATTATGTGACCGGATATTTAAATCGGGTCACTCTTTTCAGGATTTTTCCGAGCTCGCGGGAAGtttt < 6:10669/149‑3 (MQ=255) tgCCGTAAAGCAACGGCAAGTGTCCAGTTCCCGGCAATAGCCAGCACGATGTCCGAGGTTCGCGCGAAGTATGTGACCGGATATTTAAATCCGGTCACTCATTTCAGGATTTTTCCGAGCTCGCGGGAAGTTATAGACCGACTTTGCcg < 2:33036/149‑1 (MQ=255) aCGGCAAGTGTCCAGTTCCCGGCAATAGCCAGCACGATGTCCGAGGTTCGCGCGAAGTATGTGACCGGATATTTAAATCCGGTCACTCATTTCAGGATTTTTCCGAGCTCGCGGGAAGTTATAGACCGACTTTGCCGCGCACGCGCGt > 2:32872/1‑148 (MQ=255) tCCAGTTCCCGGCAATAGCCAGCACGATGTCCGAGGTTCGCGCGAAGTATGTGACCGGATATTTAAATCCGGTCACTCATTTCAGGATTTTTCCGAGCTc < 6:30691/100‑1 (MQ=255) tCCAGTTCCCGGCAATAGCCAGCACGATGTCCGAGGTTCGCGCGAAGTATGTGACCGGATATTTAAATCCGGTCACTCATTTCAGGATTTTTCCGAGCTc > 5:30691/1‑100 (MQ=255) cccGGCAATAGCCAGCACGATGTCCGAGGTTCGCGCGAAGTATGTGACCGGATATTTAAATCCGGTCACTCATTTCAGGATTTTTCCGAGCTCGCGGGAAGTTATAGACCGACTTTGCCGCGCACGCGCGTCAGAATCTGCGCGAAtt > 7:9909/1‑148 (MQ=255) ggCAATAGCCAGCACGATGTCCGAGGTTCGCGCGAAGTATGTGACCGGATATTTAAATCCGGTCACTCATTTCAGGATTTTTCCGAGCTCGCGGGAAGTTATAGACCGACTTTGCCGCGCACGCGCGTCAGAATCTGCGCGAATTCAcc < 4:5607/149‑1 (MQ=255) gCAATAGCCAGGACGATGTCCGAGGTTCGCGCGAAGTATGTGACCGGATATTTAAATCCGGTCACTCATTTCAGGATTTTTCCGAGCTCGCGGGAAGTTATAGACCGACTTTGCCGCTCACGCGCGTCAGAATCTGCGCGAATTCACCg < 2:126471/149‑1 (MQ=255) aaTAGCCAGCACGATGTCCGAGGTTCGCGCGAAGTATGTGACCGGATATTTAAATCCGGTCACACATTTCAGGATTTTTCCGAGCTCGCGGGAAGTTATAGACCGACTTTGCCGCGCACGCGCGTCAGAATCTGCGCGAATTCACCGGc < 4:130466/149‑1 (MQ=255) ccAGCACGATGTCCGAGGTTCGCGCGAAGTATGTGACCGGATATTTAAATCCGGTCACTCATTTCAGGATTTTTCCGAGCTCGCGGGAAGTTATAGACCGACTTTGCCGCGCACGCGCGTCAGAATCTGCGCGAATTCACCGGCATTTa > 1:517/1‑149 (MQ=255) aTGTCCGAGGTTCGCGCGAAGTATGTGACCGGATATTTAAATCCGGTCACTCATTTCAGGATTTTTCCGAGCTCGCGGGAAGTTATAGACCGACTTTGCCGCGCACGCGCGTCAGAATCTGCGCGAATTCACCGGCATTTATCTCGcc > 8:146161/1‑148 (MQ=255) cgcgcgAAGTATGTGACCGGATATTTAAATCCGGTCACTCATTTCAGGATTTTTCCGAGCTCCAGGGAAGTTATAGACAGACTTTGCCGCGCACGCGCGTCAGAATCTCCGCGAAGTCACCGGCATTTAACTCGCCAATCACCCCAt > 4:48940/1‑147 (MQ=255) gcgcgAAGTATGTGACCGGATATTTAAATCCGGTCACTCATTTCAGGATTTTTCCGAGCTCGCGGGAAGTTATAGACCGACTTTGCCGCGCACGCGCGTCAGAATCTGCGCGAATTCAc > 5:53714/1‑119 (MQ=255) gcgcgAAGTATGTGACCGGATATTTAAATCCGGTCACTCATTTCAGGATTTTTCCGAGCTCGCGGGAAGTTATAGACCGACTTTGCCGCGCACGCGCGTCAGAATCTGCGCGAATTCAc < 6:53714/119‑1 (MQ=255) aTGTGACCGGATATTTAAATCCGGTCACTCATTTCAGGATTTTTCCGAGCTCGCGGGAAGTTATAGACCGACTTTGCCGCGCACGCGCGTCAGAATCTGCGCGAATTCACCGGCATTTATCTCGCCAATCACCCGATCAGCGGTAAttt > 3:80218/1‑149 (MQ=255) gtgACCGGATTTAAATATCCGGTCACTCATTTCAGGATTTTTCCGAGCTCGCGGGAAGTTATAGACCGACTTTGCCGCGCACGCGCGTCAGAATCTGCGCGAATTCACCGGCATTTATCTCGCCAATCACCCGATCAGCGGTAATTTCg > 2:137869/1‑149 (MQ=255) tgACCGGATATTTAAATCCGGTCACTCATTTCAGGATTTTTCCGAGCTCGCGGGAAGTTATAGACCGACTTTGCCGCGCACGCGCGTCAGAATCTGCGCGAATTCACCGGCATTTATCTCGCCAATCACCCGATCAGCGGTAATTTCGc > 5:214382/1‑149 (MQ=255) gACCGGATATTTAAATCCGGTCACTCATTTCAGGATTTTTCCGAGCTCGCGGGAAGTTATAGACCGACTTTGCCGCGCACGCGCGTCAGAATCTGCGCGAATTCACCGGCATTTATCTCGCCAATCACCCGATCAGCGGTAATTTCGCt > 5:131197/1‑149 (MQ=255) gACCGGATATTTAAATCCGGTCACTCATTTCAGGATTTTTCCGAGCTCGCGGGAAGTTATAGACCGACTTTGCCGCGCACGCGCGTCAGAATCTGCGCGAATTCACCGGCATTTATCTCGCCAATCACCCGATCAGCGGTAATTTCGCt > 7:169368/1‑149 (MQ=255) ccGGATATTTAAATCCGGTTACTCATTTCAGGATTTTTCCGAGCTCGCGGGAAGTTATAGACCGACTTTGCCGCGCACGCGCGTCAGAATCTGCGCGAATTCACCGGCATTTATCTCGCCAATCACCCGATCAGCGGTAATTTCGCTGc > 5:23307/1‑149 (MQ=255) cGGATATTTAAATCCGGTCACTCATTTCAGGATTTTTCCGAGTTCGCGGGAAGTTATAGACCGACTTTGCCGCGCACGCGCGTCAGAATCTGCGCGAATTCACCgg > 4:10945/1‑106 (MQ=255) cGGATATTTAAATCCGGTCACTCATTTCAGGATTTTTCCGAGTTCGCGGGAAGTTATAGACCGACTTTGCCGCGCACGCGCGTCAGAATCTGCGCGAATTCACCgg < 3:10945/106‑1 (MQ=255) ggATATTTAAATCCGGTCACTCATTTCAGGATTTTTCCGAGCTCGCGGGAAGTTATAGACCGACTTTGCCGCGCACGCGCGTCAGAATCTGCGCGAATTCACCGGCATTTATCTCGCCAATCACCCGATCAGCGGTAATTTCGCTGCCg > 6:209370/1‑149 (MQ=255) gATATTTAAATCCGGTCACTCATTTCAGGATTTTTCCGAGCTCGCGGGAAGTTATAGACCGACTTTGCCGCGCACGCGCGTCAGAATCTGCGCGAATTCACCGGCATTTATCTCGCCAATCACCCGATCAGCGGTaa < 4:35605/137‑1 (MQ=255) gATATTTAAATCCGGTCACTCATTTCAGGATTTTTCCGAGCTCGCGGGAAGTTATAGACCGACTTTGCCGCGCACGCGCGTCAGAATCTGCGCGAATTCACCGGCATTTATCTCGCCAATCACCCGATCAGCGGTaa > 3:35605/1‑137 (MQ=255) tttAAATCCGGTCACTCATTTCAGGATTTTTCCGAGCTCGCGGGAAGTTATAGACCGACTTTGCCGCGCACGCGCGTCAGAATCTGCGCGAATTCACCGGCATTTATCTCGCCAATCACCCGATCAGCGGTAATTTCGCTGCCGTTCg < 6:131197/148‑1 (MQ=255) | GATTCCTTGGATTTGTAGGGCAATTCGACACAGCGCGGCGAGTGTGCCGTAAAGCAACGGCAAGTGTCCAGTTCCCGGCAATAGCCAGCACGATGTCCGAGGTTCGCGCGAAGTATGTGACCGGATATTTAAATCCGGTCACTCATTTCAGGATTTTTCCGAGCTCGCGGGAAGTTATAGACCGACTTTGCCGCGCACGCGCGTCAGAATCTGCGCGAATTCACCGGCATTTATCTCGCCAATCACCCGATCAGCGGTAATTTCGCTGCCGTTCG > NC_002947/650717‑650991 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |