Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	6,339	T→A	8.9%	Q15L (CAG→CTG)	trmE ←	GTPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	6,339	0	T	A	8.9%	150.4 / 5.7	56	Q15L (CAG→CTG)	trmE	GTPase
Reads supporting (aligned to +/- strand):  ref base T (26/25);  new base A (2/3);  total (28/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.84e-01

CCTTCGTCCAGCACCAGCCCTTCATCGTCGCGGAATGGACCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCGTTTTTTA‑TTCACAGGCTAGCGTATTAGCCCGTCAAGCAGCA  >  NC_002947/6195‑6480                                                                                                                                                 |                                                                                                                                                ccTTCGTCCAGCACCAGCCCTTCATCGTCGCGGAATGGACCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTggg                                                                                                                                              <  4:82152/148‑1 (MQ=255)       tCCAGCACCAGCCCTTCATCGTCGCGGAATGGACCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGAGCAACAGGCCGGCCTTGGCAGCCAGCGGGCCGGACAACCTGACAAATCCCACACCGCCCCGGCCCTGGGCCgacgc                                                                                                                                       >  8:234943/1‑145 (MQ=255)         cAGCACCAGCCCTTCATCGTCGCGGAATGGACCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGa                                                                                                                                     >  1:223140/1‑149 (MQ=255)         cAGCACCAGCCCTTCATCGTCGCGGAATGGACCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCACGGCCCAGGGCCGGGGCgg                                                                                                                                     <  7:145320/149‑2 (MQ=255)               cAGCCCTTCATCGTCGCGGAATGGACCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATggcgg                                                                                                                               >  3:71781/1‑149 (MQ=255)                  cttccgaTCTTCGCGGAATGGACCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATggcggc                                                                                                                              <  3:45720/141‑1 (MQ=255)                    cTTCATCGTCGCGGAATGGACCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATg                                                                                                                          >  2:198431/1‑149 (MQ=255)                    cTTCATCGTCGCGGAATGGACCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATg                                                                                                                          >  7:95416/1‑149 (MQ=255)                       cATCTTCGCGGAATTGACAGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCATCAGGCCGGCCTTGGCAGGCATAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGt                                                                                                                        <  6:242400/148‑1 (MQ=255)                        aTCGTCGCGGAATGGACCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGtt                                                                                                                       <  8:154976/148‑1 (MQ=255)                           gtcgCGGAATGGACCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCAc                                                                                                                   <  4:58785/149‑1 (MQ=255)                            tcgCGGAATGGACCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACg                                                                                                                  >  7:190294/1‑149 (MQ=255)                             cgcgGAATGGACCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATggcggc                                                                                                                              >  4:45720/1‑136 (MQ=255)                                ggAATGGACCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGcaca                                                                                                              <  4:219749/149‑1 (MQ=255)                                        ccGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCAt                                                                                                      <  8:190294/149‑1 (MQ=255)                                         cGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATg                                                                                                     >  6:53756/1‑149 (MQ=255)                                         cGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATg                                                                                                     >  2:259608/1‑149 (MQ=255)                                                ggCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGa                                                                                                >  5:55958/1‑147 (MQ=255)                                                             gTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACa                                                                                  >  8:39036/1‑148 (MQ=255)                                                              tCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGaaa                                                                                              <  5:227023/135‑1 (MQ=255)                                                              tCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGaaa                                                                                              >  6:227023/1‑135 (MQ=255)                                                                 gggTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAAc                                                                             <  3:206673/149‑1 (MQ=255)                                                                 gggTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAAc                                                                             <  7:39036/149‑1 (MQ=255)                                                                  ggTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAAc                                                                             >  7:131159/1‑148 (MQ=255)                                                                   gTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGTTGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGa                                                                           <  1:198431/149‑1 (MQ=255)                                                                   gTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGa                                                                           <  8:95416/149‑1 (MQ=255)                                                                         ccGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGa                                                                       >  3:140533/1‑147 (MQ=255)                                                                         ccGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGa                                                                       >  2:135969/1‑147 (MQ=255)                                                                                tCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCaaaaa                                                              <  4:71781/149‑1 (MQ=255)                                                                                 cAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAAc                                                                             >  1:16081/1‑133 (MQ=255)                                                                                 cAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAAc                                                                             <  2:16081/133‑1 (MQ=255)                                                                                   aCAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGAc                                                                          >  1:156402/1‑134 (MQ=255)                                                                                   aCAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGAc                                                                          <  2:156402/134‑1 (MQ=255)                                                                                       gacggccTTGGCAGCCAGAGGGCCGGACAACCTCACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATCATCCAAAGCCTCAACGACAAAAACTACAGATAGAAAAAACGcccc                                                        >  2:232672/3‑148 (MQ=255)                                                                                          ggccTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCacac                                                      >  1:268842/1‑147 (MQ=255)                                                                                             cTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAg‑gg                                                <  1:264208/149‑1 (MQ=255)                                                                                                ggCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAg‑gggg                                              <  8:131159/148‑1 (MQ=255)                                                                                                   aGCCAGAGGGCCGGAGAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCC‑CTAGTGGGGCGtt                                          <  7:234943/149‑1 (MQ=255)                                                                                                    gCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCGttt                                         <  1:232672/149‑1 (MQ=255)                                                                                                       agagGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCGtttttg                                      <  6:55958/149‑2 (MQ=255)                                                                                                             ccGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCGTTTTTTA‑TTca                                >  6:107258/1‑148 (MQ=255)                                                                                                               ggACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCGTTTTTTA‑TTCACAg                             <  1:259608/149‑1 (MQ=255)                                                                                                                      cTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCGTTTTTTA‑TTCACAg                             <  7:237238/142‑1 (MQ=255)                                                                                                                      cTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCGTTTTTTA‑TTCACAg                             >  8:237238/1‑142 (MQ=255)                                                                                                                      cTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCC‑CTAg‑tggggc                                            <  7:215662/127‑3 (MQ=255)                                                                                                                      cTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCC‑CTAg‑tggggc                                            >  8:215662/1‑125 (MQ=255)                                                                                                                           aaTTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCGTTTTTTA‑TTCACAGGCTAGCGTAt                   >  1:142180/1‑147 (MQ=255)                                                                                                                           aaTTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCGTTTTTTA‑TTCACAGGCTAGCGTAt                   >  2:58633/1‑147 (MQ=255)                                                                                                                               ccgccatcGCCACGGCCCAGGGCCGGGGCGGTGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCGTTTTTTA‑TTCACAGGCTAGCGTATTAGccc             >  2:35853/8‑149 (MQ=255)                                                                                                                                ccACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCGTTTTTTA‑TTCACAGGCTAGCGTATTAGccc             <  2:142180/148‑1 (MQ=255)                                                                                                                                 cacaCCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCGTTTTTTA‑TTCACAGGCTAGCGTATTAGCCCGt           >  3:69446/1‑149 (MQ=255)                                                                                                                                     tcGCCACGGCCCAGGGCCGGGGCGGTGTGGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCGTTTTTTA‑tt                                  >  3:239645/2‑122 (MQ=38)                                                                                                                                     tcGCCACGGCCCAGGGCCGGGGCGGTGTGGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCGTTTTTTA‑tt                                  <  4:239645/121‑1 (MQ=38)                                                                                                                                      cGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCGTTTTTTA‑TTCACAGGCTAGCGTATTAGCCCGTCAagc      <  1:135969/149‑1 (MQ=255)                                                                                                                                      cGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCGTTTTTTA‑TTCACAGGCTAGCGTATTAGCCCGTCAag       >  6:187209/1‑148 (MQ=255)                                                                                                                                           cGGCCCAGGGCCGGGGCGGTGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCGTTTTTTATTTCACAGGCTAGCGTATTAGCCCGTCAagcagca  <  1:35853/149‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                                CCTTCGTCCAGCACCAGCCCTTCATCGTCGCGGAATGGACCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCGTTTTTTA‑TTCACAGGCTAGCGTATTAGCCCGTCAAGCAGCA  >  NC_002947/6195‑6480

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑