| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 714,976 | T→C | 7.7% | intergenic (+42/+35) | ispH → / ← PP_0607 | 4‑hydroxy‑3‑methylbut‑2‑enyl diphosphate reductase/type IV pili biogenesis protein FimT |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 714,976 | 0 | T | C | 7.7% | 146.4 / 2.5 | 52 | intergenic (+42/+35) | ispH/PP_0607 | 4‑hydroxy‑3‑methylbut‑2‑enyl diphosphate reductase/type IV pili biogenesis protein FimT |
| Reads supporting (aligned to +/- strand): ref base T (24/24); new base C (2/2); total (26/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.50e-01 | |||||||||||
ACAGCTCAAGGCCTGGGGCGCCACTGGCGCTGAAGAGCTGGACGGGCGCGAAGAGAACATCACCTTTTCAATGCCCAAAGAGCTGCGGGTTCGCTCGCTGATCTGACGGGCAGTACCGCCGCCAATGCAGGGGCTGC‑TTTGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAATGACCGC‑GCTAGCAGCGGTCATTGCTCAGGTCCTCCGTAAGCAACCTGACTCTGCCGCTTGATGCCAGCACCACCTGATATCGGCTGCGAGCAGCGGCGCGTTCGCACATTTCCAGTTT > NC_002947/714829‑715121 | aCAGCTCAAGGCCTGGGGCGCCACTGGCGCTGAAGAGCTGGACGGGCGCGAAGAGAACATCACCTTTTCAATGCCCAAAGAGCTGCGGGTTCGCTCGCTGATCTGACGGGCAGTACCGCCGCCAATGCAGGGGCTGC‑TTTGCAGCCCtt > 1:117756/1‑149 (MQ=255) cTCAAGGCCTGGGGCGCCACTGGCGCTGAAGAGCTGGACGGGCGCGAAGAGAACATCACCTTTTCAATGCCCAAAGAGCTGCGGGTTCGCTCGCTGATCTGACGGGCAGTACCGCCGCCAATGCAGGGGCTGC‑TTTGCAGCCCTTgcgc > 1:119332/1‑149 (MQ=255) tCAAGGCCTGGGGCGCCACTGGCGCTGAAGAGCTGGACGGGCGCGAAGAGAACATCACCTTTTCAATGCCCAAAGAGCTGCGGGTTCGCTCGCTGATCTGACGGGCAGTACCGCCGCCAATGCAGGGGCTGC‑TTTGCAGCCCTTgcgc > 3:193267/1‑148 (MQ=255) aaGGCCTGGGGCGCCACTGGCGCTGAAGAGCTGGACGGGCGCGAAGAGAACATCACCTTTTCAATGCCCAAAGAGCTGCGGGTTCGCTCGCTGATCTGACGGGCAGTACCGCCGCCAATGCAGGGGCTGC‑TTTGCAGCCCTTgcgc < 4:193267/146‑1 (MQ=255) ggCCTGGGGCGCCACTGGCGCTGAAGAGCTGGACGGGCGCGAAGAGAACATCACCTTTTCAATGCCCAAAGAGCTGCGGGTTCGCTCGCTGATCTGACGGGCAGTACCGCCGCCAATGCAGGGGCTGC‑TTTGCAGCCCTTGCGCGacac < 2:119332/149‑1 (MQ=255) ggCCTGGGGCGCCACTGGCGCTGAAGAGCTGGACGGGCGCGAAGAGAACATCACCTTTTCAATGCCCAAAGAGCTGCGGGTTCGCTCGCTGATCTGACGGGCAGTACCGCCGCCAATGCAGGGGCTGC‑TTTGCAGCCCTTGCGCGaca > 2:70017/1‑148 (MQ=255) ggCCTGGGGCGCCACTGGCGCTGAAGAGCTGGACGGGCGCGAAGAGAACATCACCTTTTCAATGCCCAAAGAGCTGCGGGTTCGCTCGCTGATCTGACGGGCAGTACCGCCGCCAATGCAAGGGCTGC‑AAAGCAGCCCCTgcattggcg < 2:112808/149‑8 (MQ=255) cTGGGGCGCCACTGGCGCTGAAGAGCTGGACGGGCGCGAAGAGAACATCACCTTTTCAATGCCCAAAGAGCTGCGGGTTCGCTCGCTGATCTGACGGGCAGTACCGCCGCCAATGCAGGGGCTGC‑TTTGCAGCCCTTGCGCGacac > 4:146571/1‑146 (MQ=255) gggCGCCACTGGCGCTGAAGAGCTGGACGGGCGCGAAGAGAACATCACCTTTTCAATGCCCAAAGAGCTGCGGGTTCGCTCGCTGATCTGACGGGCAGTACCGCCGCCAATGCAGGGGCTGC‑TTTGCAGCCCTTGCGCGACACAAGa < 3:30833/147‑1 (MQ=255) aaGAGCTGGACGGGCGCGAAGAGAACATCACCTTTTCAATGCCCAAAGAGCTGCGGGTTCGCTCGCTGATCTGACGGGCAGTACCGCCGCCAATGCAGGGGCTGC‑TTTGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAATGACcg < 2:89311/148‑1 (MQ=255) agCTGGACGGGCGCGAAGAGAACATCACCTTTTCAATGCCCAAAGAGCTGCGGGTTCGCTCGCTGATCTGACGGGCAGTACCGCCGCCAATGCAGGGGCTGC‑TTTGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAATGACcgctgc > 3:67982/1‑146 (MQ=255) tGGACGGGCGCGAAGAGAACATCACCTTTTCAATGCCCAAAGAGCTGCGGGTTCGCTCGCTGATCTGACGGGCAGTACCGCCGCCAATGCAGGGGCTGC‑TTTGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAATGACCGC‑GCTag > 3:57057/1‑148 (MQ=255) aCGGGCGCGAAGAGAACATCACCTTTTCAATGCCCAAAGAGCTGCGGGTTCGCTCGCTGATCTGACGGGCAGTACCGCCGCCAATGCAGGGGCTGC‑TTTGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAATGACCGC‑GCTagcagc < 5:205502/149‑1 (MQ=255) aCGGGCGCGAAGAGAACATCACCTTTTCAATGCCCAAAGAGCTGCGGGTTCGCTCGCTGATCTGACGGGCAGTACCGCCGCCAATGCAGGGGCTGC‑TTTGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAATGACCGC‑GCTagcagc < 1:238516/149‑1 (MQ=255) gggCGCGAAGAGAACATCACCTTTTCAATGCCCAAAGAGCTGCGGGTTCGCTCGCTGATCTGACGGGCAGTACCGCCGCCAATGCAGGGGCTGCTTTTGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAATGACCGC‑GCTAGCAGCg < 1:67307/149‑1 (MQ=255) ggCGCGAAGAGAACATCACCTTTTCAATGCCCAAAGAGCTGCGGGTTCGCTCGCTGATCTGACGGGCAGTACCGCCGCCAATGCAGGGGCTGC‑TTTGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAATGACCGC‑GCTAGCAGCGGt < 4:57057/149‑1 (MQ=255) gcgAAGAGAACATCACCTTTTCAATGCCCAAAGAGCTGCGGGTTCGCTCGCTGATCTGACGGGCAGTACCGCCGCCAATGCAGGGGCTGC‑TTTGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAATGACCGC‑GCTAGCAGCGGTCAt < 8:2071/149‑1 (MQ=255) gcgAAGAGAACATCACCTTTTCAATGCCCAAAGAGCTGCGGGTTCGCTCGCTGATCTGACGGGCAGTACCGCCGCCAATGCAGGGGCTGC‑TTTGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAATGACCGC‑GCTAGCAGCGGTCAt < 5:147215/149‑1 (MQ=255) aaGAGAACATCACCTTTTCAATGCCCAAAGAGCTGCGGGTTCGCTCGCTGATCTGACGGGCAGTACCGCCGCCAATGCAGGGGCTGC‑TTTGAAGCCCTTGCGCGACACAAGACCGCTCCTGCAATGACCGC‑GCt > 7:53178/1‑134 (MQ=255) agagAACATCACCTTTTCAATGCCCAAAGAGCTGCGGGTTCGCTCGCTGATCTGACGGGCAGTACCGCCGCCAATGCAGGGGCTGC‑TTTGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAATGACCGCTGCTAGC‑GCGGTCATTGc < 4:67982/148‑1 (MQ=255) agagAACATCACCTTTTCAATGCCCAAAGAGCTGCGGGTTCGCTCGCTGATCTGACGGGCAGTACCGCCGCCAATGCAGGGGCTGC‑TTTGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAATGACCGC‑GCTAGCAGCGGTCATTGCt < 7:104843/149‑1 (MQ=255) gaACATCACCTTTTCAATGCCCAAAGAGCTGCGGGTTCGCTCGCTGATCTGACGGGCAGTACCGCCGCCAATGCAGGGGCTGC‑TTTGCAGCCCTTGCGCGCCACAAGACCGCTCCTGCAATGACCGC‑GCTAGCAGCGGTCATTGCTCAg > 2:206074/1‑149 (MQ=255) cACCTTTTCAATGCCCAAAGAGCTGCGGGTTCGCTCGCTGATCTGACGGGCAGTACCGCCGCCAATGCAGGGGCTGC‑TTTGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAATGACCGCTGCTAGC‑GCGGTCATTGcaggagc > 5:211172/1‑139 (MQ=39) cACCTTTTCAATGCCCAAAGAGCTGCGGGTTCGCTCGCTGATCTGACGGGCAGTACCGCCGCCAATGCAGGGGCTGC‑TTTGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAATGACCGCTGCTAGC‑GCGGTCATTGcaggagc < 6:211172/145‑7 (MQ=39) cTTTTCAATGCCCAAAGAGCTGCGGGTTCGCTCGCTGATCTGACGGGCAGTACCGCCGCCAATGCAGGGGCTGC‑TTTGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAATGACCGC‑GCTAGCAGCGGTCATTGCTCAGGTCCTCCg > 1:156890/1‑148 (MQ=255) ttCAATGCCCAAAGAGCTGCGGGTTCGCTCGCTGATCTGACGGGCAGTACCGCCGCCAATGCAGGGGCTGC‑TTTGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAATGACCGC‑GCTAGCAGCGGTCATTGCTCAGGTCCTCCGTaa < 3:146571/148‑1 (MQ=255) aaaGAGCTGCGGGTTCGCTCGCTGATCTGACGGGCAGTACCGCCGCCAATGCAGGGGCTGC‑TTTGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAATGACCGC‑GCTAGCAGCGGTCATTGCTCAGGTCCTCCGTAAGCAACCTGAc > 4:74792/1‑148 (MQ=255) ttCGCTCGCTGATCTGACGGGCAGTACCGCCGCCAATGCAGGGGCTGC‑TTTGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAATGACCGC‑GCTAGCAGCGGTCATTGCTCAGGTCCTCCGTAAGCAACCTGACTCTGCCGCTTGATg > 5:88722/1‑149 (MQ=255) gctcgctGATCTGACGGGCAGTACCGCCGCCAATGCAGGGGCTGC‑TTTGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAATGACCGC‑GCTAGCAGCGGTCATTGCTCAGGTCCTCCGTAAGCAACCTGAct < 3:21930/133‑1 (MQ=255) gctcgctGATCTGACGGGCAGTACCGCCGCCAATGCAGGGGCTGC‑TTTGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAATGACCGC‑GCTAGCAGCGGTCATTGCTCAGGTCCTCCGTAAGCAACCTGAct > 4:21930/1‑133 (MQ=255) ctcgctGATCTGACGGGCAGTACCGCCGCCAATGCAGGGGCTGC‑TTTGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAATGACCGC‑GCTAGCAGCGGTCATTGCTCAGGTCCTCCGTAAGCAACCTGACTCTGCCGCTTGATGCCAg < 1:63482/149‑1 (MQ=255) ctcgctGATCTGACGGGCAGTACCGCCGACAATGCAGGGGCTGC‑TTTGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAATGACCGC‑GCTAGCAGCGGTCATTGCTCAGGTCCTCCGTAAGCAACCTGACTCTGCCGCTTGATGCCAg < 3:4910/149‑1 (MQ=255) tcgctGATCTGACGGGCAGTACCGCCGCCAATGCAGGGGCTGC‑TTTGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAATGACCGC‑GCTAGCAGCGGTCATTGCTCAGGTCCTCCGTAAGCAACCTGACTCTGCCGCTTGATGCCAGc > 8:217257/1‑149 (MQ=255) tCTGACGGGCAGTACCGCCGCCAATGCAGGGGCTGC‑TTTGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAATGACCGC‑GCTAGCAGCGGTCATTGCTCAGGTCCTCCGTAAGCAACCTGACTCTGCCGCTTGATGCCa > 4:128864/1‑140 (MQ=255) tCTGACGGGCAGTACCGCCGCCAATGCAGGGGCTGC‑TTTGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAATGACCGC‑GCTAGCAGCGGTCATTGCTCAGGTCCTCCGTAAGCAACCTGACTCTGCCGCTTGATGCCa < 3:128864/140‑1 (MQ=255) gggCAGTACCGCCGCCAATGCAGGGGCTGC‑TTTGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAATGACCGC‑GCTAGCAGCGGTCATTGCTCAGGTCCTCCGTAAGCAACCTGACTCTGCCGCTTGATGCCAGCACCACCTGATATc < 3:74792/149‑1 (MQ=255) gCAGTACCGCCGCCAATGCAGGGGCTGC‑TTTGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAATGACCGC‑GCTAGCAGCGGTCATTGCTCAGGTCCTCCGTAAGCAACCTGACTCTGCCGCTTGATGCCAGCACCACCTGATATCgg > 1:226800/1‑149 (MQ=255) ccgccAATGCAGGGGCTGC‑TTTGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAATGACCGC‑GCTAGCAGCGGTCATTGCTCAGGTCCTCCGTAAGCAACCTGAct < 1:68789/107‑1 (MQ=255) ccgccAATGCAGGGGCTGC‑TTTGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAATGACCGC‑GCTAGCAGCGGTCATTGCTCAGGTCCTCCGTAAGCAACCTGAct > 2:68789/1‑107 (MQ=255) tctcgcgCAAGGGCTGC‑AAAGCAGCCCCTGCGCGACACAAGACCGCTCCTGCAATGACCGC‑GCTAGCAGCGGTCATTGCTCAGGTCCTCCGTAAGCAACCTGACTCTGCCGCTTGATGCCAGCACCACCTGATATCGGCTGCGagcag < 1:114268/142‑1 (MQ=255) aaTTCAGGGGCTGC‑TTTGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAATGACCGC‑GCTAGCAGCGGTCATTGCTCAGGTCCTCCGTAAGCAACCTGACTCTGCCGCTTGATGCCAGcacc > 5:35087/1‑123 (MQ=255) aaTTCAGGGGCTGC‑TTTGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAATGACCGC‑GCTAGCAGCGGTCATTGCTCAGGTCCTCCGTAAGCAACCTGACTCTGCCGCTTGATGCCAGcacc < 6:35087/123‑1 (MQ=255) gcgCAAGGGCTGC‑AGAGCAGCCCCTGCGCGACACAAGACCGCTCCTGCAATGACCGC‑GCTAGCAGCGGTCATTGCTCAGGTCCTCCGTAAGCAACCTGACTCTGCCGCTTGATGCCAGCACCACCTGATATCGGCTGCGAGCAgcggcg > 7:228188/3‑149 (MQ=255) gcgCAAGGGCTGC‑AAAGCAGCCCCTGCGCGACACAAGACCGCTCCTGCAATGACCGC‑GCTAGCAGCGGTCATTGCTCAGGTCCTCCGTAAGCAACCTGACTCTGCCGCTTGATGCCAGCACCACCTGATATCGGCTGCGagcag > 2:114268/3‑144 (MQ=255) gCAAGGGCTGC‑AAAGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAATGACCGC‑GCTAGCAGCGGTCATTGCTCAGGTCCTCCGTAAGCAACCTGACTCTGCCGCTTGATGCCAGCACCACCTGATATCGGCTGCGAGCAGCGgcgc < 8:64877/148‑1 (MQ=255) gCAAGGGCTGC‑AAAGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAATGACCGC‑GCTAGCAGCGGTCATTGCTCAGGTCCTCCGTAAGCAACCTGACTCTGCCGCTTGATGCCAGCACCACCTGATATCGGCTGCGAGCAGCGGcgcg > 7:64877/1‑149 (MQ=255) ggggCTGC‑TTTGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAATGACCGC‑GCTAGCAGCGGTCATTGCTCAGGTCCTCCGTAAGCAACCTGACTCTGCCGCTTGATGCCAGCACCACCTGATATCGGCTGCGAGCAGCGGCGCGTTc > 7:143172/1‑149 (MQ=255) agggCTGC‑AAAGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAATGACCGC‑GCTAGCAGCGGTCATTGCTCAGGTCCTCCGTAAGCAACCTGACTCTGCCGCTTGATGCCAGCACCACCTGATATCGGCTGCGAGCAGCGGCGCGTTc > 3:51162/2‑149 (MQ=255) tgc‑tTTGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAATGACCGC‑GCTAGCAGCGGTCATTGCTCAGGTCCTCCGTAAGCAACCTGACTCTGCCGCTTGATGCCAg > 5:171790/1‑108 (MQ=255) tgc‑tTTGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAATGACCGC‑GCTAGCAGCGGTCATTGCTCAGGTCCTCCGTAAGCAACCTGACTCTGCCGCTTGATGCCAGCACCACCTGATATCGGCTGCGAGCAGCGGCGCGTTCGcaca > 3:215866/1‑149 (MQ=255) cAGCCCTTGCGCGACACAAGACCGCTCCTGCAATGACCGC‑GCTAGCAGCGGTCATTGCTCAGGTCCTCCGTAAGCAACCTGACTCTGCCGCTTGATGCCAGCACCACCTGATATCGGCTGCGAGCAGCGGCGCGTTCGCACATTTCCa < 6:88722/148‑1 (MQ=255) aGCCCTTGCGCGACACACGCCCCCTCCTGCAATGACCGC‑GCTAGCAGCGGTCATTGCTCAGGTCCTCCGTAAGCAACCTGACTCTGCCGCTTGATGCCAGCACCACCTGATATCGGCTGCGAGCAGCGGCGCGTTCGCACATTTCCAGt < 6:149369/149‑1 (MQ=255) cccTTGCGCGACACAAGACCGCTCCTGCAATGACCGC‑GCTAGCAGCGGTCATTGCTCAGGTCCTCCGTAAGCAACCTGACTCTGCCGCTTGATGCCAGCACCAACTGATATCGGCTGCGAGCAGCGGCGCGTTCGCACATTTCCAGttt > 6:151355/1‑149 (MQ=255) | ACAGCTCAAGGCCTGGGGCGCCACTGGCGCTGAAGAGCTGGACGGGCGCGAAGAGAACATCACCTTTTCAATGCCCAAAGAGCTGCGGGTTCGCTCGCTGATCTGACGGGCAGTACCGCCGCCAATGCAGGGGCTGC‑TTTGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAATGACCGC‑GCTAGCAGCGGTCATTGCTCAGGTCCTCCGTAAGCAACCTGACTCTGCCGCTTGATGCCAGCACCACCTGATATCGGCTGCGAGCAGCGGCGCGTTCGCACATTTCCAGTTT > NC_002947/714829‑715121 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |