Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 725,706 A→C 6.2% L65V (TTG→GTG)  PP_0619 ← branched‑chain amino acid ABC transporter substrate‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947725,7060AC 6.2% 203.7 / 3.6 64L65V (TTG→GTG) PP_0619branched‑chain amino acid ABC transporter substrate‑binding protein
Reads supporting (aligned to +/- strand):  ref base A (32/28);  new base C (2/2);  total (34/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01

GACACTGCCAGTGCCGAAGACGACAGGGTGCACCCCTGGAAGAAGCGTGCACCATCCTGGCCGATGGCTTCCTGCACCTTGCGCACCGCCTTGCCGGCGTTGCCTTCGGTGTCGATGACTTTGTACTTGAGCGGGTGGCCGAGCAACTGCGGGTGCTCGGCGATGGCCAGGCGCGAGCCCATGTCGGCGTACTTGCCGTAGCTGGCGAAGGCGCCCGACATGGACTTGAGCCCATAGAAGGTCAGCGGTTGGTCGGCGAATGCCCGGTGTGCCCCCAAGGGGAGGCTACCTAAG  >  NC_002947/725561‑725854
                                                                                                                                                 |                                                                                                                                                    
gACACTGCCAGTGCCGAAGACGACAGGGTGCACCCCTGGAAGAAGCGTGCACCATCCTGGCCGATGGCTTCCTGCACCTTGCGCACCGCCTTGCCGGCGTTGCCTTCGGTGTCGATGACTTTGTACTTGAGCGGGTGGCCGAGCAACt                                                                                                                                                    <  6:102843/148‑1 (MQ=255)
   acTGCCAGTGCCGAAGACGACAGGGTGCACCCCTGGAAGAAGCGTGCACCATCCTGGCCGATGGCTTCCTGCACCTTGCGCACCGCCTTGCCGGCGTTGCCTTCGGTGTCGATGACTTTGTACTTGAGCGGGTGGCCGAGCAACTGCgg                                                                                                                                                <  2:174312/149‑1 (MQ=255)
              cGAAGACGACAGGGTGCACCCCTGGAAGAAGCGTGCACCATCCTGGCCGATGGCTTCCTGCACCTTGCGCACCGCCTTGCCGGCGTTGCCTTCGGTGTCGATGACTTTGTACTTGAGCGGGTGGCCGAGCAACTGCGGGTGCTCGGCGa                                                                                                                                     >  3:234945/1‑149 (MQ=255)
              cGAAGACGACAGGGTGCACCCCTGGAAGAAGCGTGCACCATCCTGGCCGATGGCTTCCTGCACCTTGCGCACCGCCTTGCCGGCGTTGCCTTCGGTGTCGATGACTTTGTACTTGAGCGGGTGGCCGAGCAACTGCGGGTGCTCGGCGa                                                                                                                                     >  6:35328/1‑149 (MQ=255)
               gAAGACGACAGGGTGCACCCCTGGAAGAAGCGTGCACCATCCTGGCCGATGGCTTCCTGCACCTTGCGCACCGCCTTGCCGGCGTTGCCTTCGGTGTCGATGACTTTGTACTTGAGCGGGTGGCCGAGCAACTGCGGGTGCTCGGCGAt                                                                                                                                    <  5:205141/149‑1 (MQ=255)
               gAAGACGACAGGGTGCACCCCTGGAAGAAGCGTGCACCATCCTGGCCGATGGCTTCCTGCACCTTGCGCACCGCCTTGCCGGCGTTGCCTTCGGTGTCGATGACTTTGTACTTGAGCGGGTGGCCGAGCAACTGCGGGTGCTCGGCGAt                                                                                                                                    >  6:205141/1‑149 (MQ=255)
                aaGACGACAGGGTGCACCCCTGGAAGAAGCGTGCACCATCCTGGCCGATGGCTTCCTGCACCTTGCGCACCGCCTTGCCGGCGTTGCCTTCGGTGTCGATGACTTTGTACTTGAGCGGGTGGCCGAGCAACTGCGGGTGCTCGGCGATg                                                                                                                                   <  5:227147/149‑1 (MQ=255)
                  cttccaggggTGCA‑CCCTGGAAGAAGCGTGCACCATCCTGGCCGATGGCTTCCTGCACCTTGCGCACCGCCTTGCCGGCGTTGCCTTCGGTGTCGATGACTTTGTACTTGAGCGGGTGGCCGAGCAACTGCGGGTGCTCGGCGATGGc                                                                                                                                 >  4:55407/8‑148 (MQ=255)
                   acgacAGGGTGCACCCCTGGAAGAAGCGTGCACCATCCTGGCCGATGGCTTCCTGCACCTTGCGCACCGCCTTGCCGGCGTTGCCTTCGGTGTCGATGACTTTGTACTTGAGCGGGTGGCCGAGCAACTGCGGGTGCTCGGCGATGGc                                                                                                                                 >  2:221365/1‑148 (MQ=255)
                        aGGGTGCACCCCTGGAAGAAGCGTGCACCATCCTGGCCGATGGCTTCCTGCACCTTGCGCACCGCCTTGCCGGCGTTGCCTTCGGTGTCGATGACTTTGTACTTGAGCGGGTGGCCGAGCAACTGCgg                                                                                                                                                <  1:209888/128‑1 (MQ=255)
                        aGGGTGCACCCCTGGAAGAAGCGTGCACCATCCTGGCCGATGGCTTCCTGCACCTTGCGCACCGCCTTGCCGGCGTTGCCTTCGGTGTCGATGACTTTGTACTTGAGCGGGTGGCCGAGCAACTGCgg                                                                                                                                                >  2:209888/1‑128 (MQ=255)
                          ggTGCACTCCTGGAAGAAGCGTGCACCATCCTGGCCGATGGCTTCCTGCACCTTGCGCACCGCCTTGCCGGCGTTGCCTTCGGTGTCGATGACTTTGTACTTGAGCGGGTGGCCGAGCAACTGCGGGTGCTCGGCGATGGCCAGgc                                                                                                                            <  2:131869/146‑1 (MQ=255)
                          ggTGCACTCCTGGAAGAAGCGTGCACCATCCTGGCCGATGGCTTCCTGCACCTTGCGCACCGCCTTGCCGGCGTTGCCTTCGGTGTCGATGACTTTGTACTTGAGCGGGTGGCCGAGCAACTGCGGGTGCTCGGCGATGGCCAGgc                                                                                                                            >  1:131869/1‑146 (MQ=255)
                              gcacccTGGAAGAAGCGTGCACCATCCTGGCCGATGGCTTCCTGCACCTTGCGCACCGCCTTGCCGGCGTTGCCTTCGGTGTCGATGACTTTGTACTTGAGCGGGTGGCCGAGCAACTGCGGGTGCTCGGCGATGGCCAGGCGCGAGcc                                                                                                                     <  3:55407/146‑1 (MQ=255)
                                  ccTGGAAGAAGCGTGCACCATCCTGGCCGATGGCTTCCTGCACCTTGCGCACCGCCTTGCCGGCGTTGCCTTCGGTGTCGATGACTTTGTACTTGAGCGGGTGGCCGAGCAACTGCGGGTGCTCGGCGATGGCCAGGCGCGAGCCCATg                                                                                                                 >  6:198402/1‑149 (MQ=255)
                                       aagaagCGTGCACCATCCTGGCCGATGGCTTCCTGCACCTTGCGCACCGCCTTGCCGGCGTTGCCTTCGGTGTCGATGACTTTGTACTTGAGCGGGTGGCCGAGCACCCGCAGTTGCTCg                                                                                                                                         >  3:85941/1‑120 (MQ=255)
                                       aagaagCGTGCACCATCCTGGCCGATGGCTTCCTGCACCTTGCGCACCGCCTTGCCGGCGTTGCCTTCGGTGTCGATGACTTTGTACTTGAGCGGGTGGCCGAGCACCCGCAGTTGCTCg                                                                                                                                         <  4:85941/120‑1 (MQ=255)
                                       aagaagCGTGCACCATCCTGGCCGATGGCTTCCTGCACCTTGCGCACCGCCTTGCCGGCGTTGCCTTCGGTGTCGATGACTTTGTACTTGAGCGGGTGGCCGAGCAACTGCGGGTGCTCGGCGATGGCCAGGCGCGAGCCCATGTCGGc                                                                                                            <  2:220527/149‑1 (MQ=255)
                                       aagaagCGTGCACCATCCTGGCCGATGGCTTCCTGCACCTTGCGCACCGCCTTGCCGGCGTTGCCTTCGGTGTCGATGACTTTGTACTTGAGCGGGTGGCCGAGCAACTGCGGGTGCTCGGCGATGGCCAGGCGCGAGCCCATGTCGGc                                                                                                            <  6:41729/149‑1 (MQ=255)
                                              gTGCACCATCCTGGCCGATGGCTTCCTGCACCTTGCGCACCGCCTTGCCGGCGTTGCCTTCGGTGTCGATGACTTTGTACTTGAGCGGGTGGCCGAGCAACTGCGGGTGCTCGGCGATGGCCAGGCGCGAGCCCATGTCGGCGTACTTg                                                                                                     >  3:170997/1‑149 (MQ=255)
                                               tGCACCATCCTGGCCGATGGCTTCCTGCACCTTGCGCACCGCCTTGCCGGCGTTGCCTTCGGTGTCGATGACTTTGTACTTGAGCGGGTGGCCGAGCAACTGCGGGTGCTCGGCGATGGCCAGGCGCGAGCCCATGTCGGCGTACTTGc                                                                                                    >  7:80611/1‑149 (MQ=255)
                                                    cATCCTGGCCGATGGCTTCCTGCACCTTGCGCACCGCCTTGCCGGCGTTGCCTTCGGTGTCGATGACTTTGTACTTGAGCGGGTGGCCGAGCAACTGCGGGTGCTCGGCGATGGCCAGGCGCGAGCCCATGTCGGCGTACTTGCCGTAg                                                                                               <  5:198402/149‑1 (MQ=255)
                                                            ccGATGGCTTCCTGCACCTTGCGCACCGCCTTGCCGGCGTTGCCTTCGGTGTCGATGACTTTGTACTTGAGCGGGTGGCCGAGCAACTGCGGGTGCTCGGCGATGGCCAGGCGCGAGCCCATGTCGGCGTACTTGCAGTAGCTGGCg                                                                                         >  1:260246/1‑147 (MQ=255)
                                                                tGGCTTCCTGCACCTTGCGCACCGCCTTGCCGGCGTTGCCTTCGGTGTCGATGACTTTGTACTTGAGCGGGTGGCCGAGCAACTGCGGGTGCTCGGCGATGGCCAGGCGCGAGCCCATGTCGGCGTACTTGCCGTAGCTGGCGAAGgcg                                                                                   <  1:221365/149‑1 (MQ=255)
                                                                    ttCCTGCACCTTGCGCACCGCCTTGCCGGCGTTGCCTTCGGTGTCGATGACTTTGTACTTGAGCGGGTGGCCGAGCAACTGCGGGTGCTCGGCGATGGCCAGGCGCGAGCCCATGTCGGCGTACTTGCCGTAGCTGGCGAAGGCGccc                                                                                >  5:146153/1‑148 (MQ=255)
                                                                       cTGCACCTTGCGCACCGCCTTGCCGGCGTTGCCTTCGGTGTCGATGACTTTGTACTTGAGCGGGTGGCCGAGCAACTGCGGGTGCTCGGCGATGGCCAGGCGCGAGCCCATGTCGGCGTACTTGCCGTAGCTGGCGAAGGCGCCCGAc                                                                             <  4:234945/148‑1 (MQ=255)
                                                                         gCACCTTGCGCACCGCCTTGCCGGCGTTGCCTTCGGTGTCGATGACTTTGTACTTGAGCGGGTGGCCGAGCAACTGCGGGTGCTCGGCGATGGCCAGGCGCGAGCCCATGTCGGCGTACTTGCCGTAGCTGGCGAAGGCGCCCGACATg                                                                          >  6:81245/1‑149 (MQ=255)
                                                                          cACCTTGCGCACCGCCTTGCCGGCGTTGCCTTCGGTGTCGATGACTTTGTACTTGAGCGGGTGGCCGAGCAACTGCGGGTGCTCGGCGATGGCCAGGCGCGAGCCCATGTCGGCGTACTTGCCGTAGCTGGCGAAGGCTCCCGACATgg                                                                         <  6:13541/149‑1 (MQ=255)
                                                                          cACCTTGCGCACCGCCTTGCCGGCGTTGCCTTCGGTGTCGATGACTTTGTACTTGAGCGGGTGGCCGAGCAACTGCGGGTGCTCGGCGATGGCCAGGCGCGAGCCCATGTCGGCGTACTTGCCGTAGCTGGCGAAGGCGCCCGACATg                                                                          >  7:78272/1‑148 (MQ=255)
                                                                               tGCGCACCGCCTTGCCGGCGTTGCCTTCGGTGTCGATGACTTTGTACTTGAGCGGGTGGCCGAGCAACTGCGGGTGCTCGGCGATGGCCAGGCGCGAGCCCATGTCGGCGTACTTGCCGTAGCTGGCGAAGGCGCCCGACATGGACtt                                                                     >  3:90123/1‑148 (MQ=255)
                                                                                 cgcACCGCCTTGCCGGCGTTGCCTTCGGTGTCGATGACTTTGTACTTGAGCGGGTGGCCGAGCAACTGCGGGTGCTCGGCGATGGCCAGGCGCGAGCCCATGTCGGCGTACTTGCCGTAGCTGGCGAAGGCGCCCGACATGGACTTGa                                                                   <  3:148628/148‑1 (MQ=255)
                                                                                  tctcggCCTTGGCGGGGTTGCTTTCGGTTTCGTTGTGTTTTTAGTTGTGGGGGTGGCCGAGCTACTGGGGGTGCTGGGCTTTGGCCTGGCGCGAGCCCATGTCGGCTTACTTGCCGTTGGTGGCGAAGGCGCCGGACTTGGATTTTAGc                                                                 <  2:260246/144‑1 (MQ=255)
                                                                                   cACCGCCTTGCCGGCGTTGCCTTCGGTGTCGATGACTTTGTACTTGAGCGGGTGGCCGAGCAACTGCGGGTGCTCGGCGATGGCCAGGCGCGAGCCCATGTCGGCGTACTTGCCGTAGCTGGCGAAGGCGCCCGAc                                                                             >  7:222804/1‑136 (MQ=255)
                                                                                       gCCTTGCCGGCGTTGCCTTCGGTGTCGATGACTTTGTACTTGAGCGGGTGGCCGAGCAACTGCGGGTGCTCGGCGATGGCCAGGCGCGAGCCCATGTCGGCGTACTTGCCGTAGCTGGCGAAGGCGCCCGACATGGACTTGAGCCCATa                                                            <  4:170997/149‑1 (MQ=255)
                                                                                       gCCTTGCCGGCGTTGCCTTCGGTGTCGATGACTTTGTACTTGAGCGGGTGGCCGAGCAACTGCGGGTGCTCGGCGATGGCCAGGCGCGAGCCCATGTCGGCGTACTTGCCGTAGCTGGCGAAGGCGCCCGACATGGACTTGAGCCCATa                                                            <  7:97355/149‑1 (MQ=255)
                                                                                             ccGGCGTTGCCTTCGGTGTCGATGACTTTGTACTTGAGCGGGTGGCCGAGCAACTGCGGGTGCTCGGCGATGGCCAGGCGCGAGCCCATGTCGGCGTACTTGCCGTAGCTGGCGAAGGCGCCCGACATGGACTTGAGCCCATAGAAGGt                                                      <  5:35328/149‑1 (MQ=255)
                                                                                               ggCGTTGCCTTCGGTGTCGATGACTTTGTACTTGAGCGGGTGGCCGAGCAACTGCGGGTGCTCGGCGATGGCCAGGCGCGAGCCCATGTCGGCGTACTTGCCGTAGCTGGCGAAGGCGCCCGACATGGACTTGAGCCCATAGAAGGt                                                      >  5:204485/1‑147 (MQ=255)
                                                                                                gctgtGCCTTCGGTGTCGATGACTTTGTACTTGAGCGGGTGGCCGAGCAACTGCGGGTGCTCGGCGATGGCCAGGCGCGAGCCCATGTCGGCGTACTTGCCGTAGCTGGCGAAGGCGCCCGACATGGACTTGAGCCCATAGAAGGTCAg                                                   <  4:180372/145‑1 (MQ=255)
                                                                                                gCGTTGCCTTCGGTGTCGATGACTTTGTACTTGAGCGGGTGGCCGAGCAACTGCGGGTGCTCGGCGATGGCCAGGCGCGAGCCCATGTCGGCGTACTTGCCGTAGCTGGCGAAGGCGCCCGACATGGACTTGAGCCCATAGAAGGTCAg                                                   <  8:80611/149‑1 (MQ=255)
                                                                                                      ccTTCGGTGTCGATGACTTTGTACTTGAGCGGGTGGCCGAGCAACTGCGGGTGCTCGGCGATGGCCAGGCGCGAGCCCATGTCGGCGTACTTGCCGTAGCTGGCGAAGGCGCCCGACATGGACTTGAGCCCATAGAAGGTCAGCGGTTg                                             <  4:90123/149‑1 (MQ=255)
                                                                                                        ttCGGTGTCGATGACTTTGTACTTGAGCGGGTGGCCGAGCAACTGCGGGTGCTCGGCGATGGCCAGGCGCGAGCCCATGTCGGCGTACTTGCCGTAGCTGGCGAAGGCGCCCGACATGGACTTGAGCCCATAGAAGGTCAGCGGt                                               <  1:69888/145‑1 (MQ=255)
                                                                                                        ttCGGTGTCGATGACTTTGTACTTGAGCGGGTGGCCGAGCAACTGCGGGTGCTCGGCGATGGCCAGGCGCGAGCCCATGTCGGCGTACTTGCCGTAGCTGGCGAAGGCGCCCGACATGGACTTGAGCCCATAGAAGGTCAGCGGt                                               >  2:69888/1‑145 (MQ=255)
                                                                                                             tgtCGATGACTTTGTACTTGAGCGGGTGGCCGAGCAACTGCGGGTGCTCGGCGATGGCCAGGCGCGAGCCCATGTCGGCGTACTTGCCGTAGCTGGCGAAGGCGCCCGACATGGACTTGAGCCCATAGAAGGTCAGCGGTTGGTCGGc                                       >  5:127456/1‑148 (MQ=255)
                                                                                                               tCGATGACTTTGTACTTGAGCGGGTGGCCGAGCAACTGCGGGTGCTCGGCGATGGCCAGGCGCGAGCCCATGTCGGCGTACTTGCCGTAGCTGGCGAAGGCGCCCGACATGGACTTGAGCCCATAGAAg                                                        >  8:224865/1‑129 (MQ=255)
                                                                                                               tCGATGACTTTGTACTTGAGCGGGTGGCCGAGCAACTGCGGGTGCTCGGCGATGGCCAGGCGCGAGCCCATGTCGGCGTACTTGCCGTAGCTGGCGAAGGCGCCCGACATGGACTTGAGCCCATAGAAGGTCAGCGGTTGGTCGGCGaa                                    <  1:138194/149‑1 (MQ=255)
                                                                                                               tCGATGACTTTGTACTTGAGCGGGTGGCCGAGCAACTGCGGGTGCTCGGCGATGGCCAGGCGCGAGCCCATGTCGGCGTACTTGCCGTAGCTGGCGAAGGCGCCCGACATGGACTTGAGCCCATAGAAGGTCAGCGGTTGGTCGGCGaa                                    >  2:147696/1‑149 (MQ=255)
                                                                                                                 gATGACTTTGTACTTGAGCGGGTGGCCGAGCAACTGCGGGTGCTCGGCGATGGCCAGGCGCGAGCCCATGTCGGCGTACTTGCCGTAGCTGGCGAAGGCGCCCGACATGGACTTGAGCCCATAGAAGGTCAGCGGTTGGTCGGCGAATg                                  >  4:27283/1‑149 (MQ=255)
                                                                                                                 gATGACTTTGTACTTGAGCGGGTGGCCGAGCAACTGCGGGTGCTCGGCGATGGCCAGGCGCGAGCCCATGTCGGCGTACTTGCCGTAGCTGGCGAAGGCGCCCGACATGGACTTGAGCCCATAGAAGGTCAGCGGTTGGTCGGCGAATg                                  >  5:110640/1‑149 (MQ=255)
                                                                                                                  aTGACTTTGTACTTGAGCGGGTGGCCGAGCAACTGCGGGTGCTCGGCGATGGCCAGGCGCGAGCCCATGTCGGCGTACTTGCCGTAGCTGGCGAAGGCGCCCGACATGGAc                                                                       <  8:52525/111‑1 (MQ=255)
                                                                                                                  aTGACTTTGTACTTGAGCGGGTGGCCGAGCAACTGCGGGTGCTCGGCGATGGCCAGGCGCGAGCCCATGTCGGCGTACTTGCCGTAGCTGGCGAAGGCGCCCGACATGGAc                                                                       >  7:52525/1‑111 (MQ=255)
                                                                                                                  aTGACTTTGTACTTGAGCGGGTGGCCGAGCAACTGCGGGTGCTCGGCGATGGCCAGGCGCGAGCCCATGTCGGCGTACTTGCCGTAGCTGGCGAAGGCGCCCGACATGGACTTGAGCCCATAGAAGGTCAGCGGTTGGTCGGCGAATGc                                 >  5:31679/1‑149 (MQ=255)
                                                                                                                       tttGTACTTGAGCGGGTGGCCGAGCAACTGNGGGTGCTCGGAGATGGCCAGGCGCGAGCCCATGTAGGCGTACTTGACGTAGCTGGAGAAGGCGCCCGACATGGACTTGAGCCCAAAGAAGTTCAGCTGTTGGTCGGCGAATGCCCg                              >  1:22615/1‑147 (MQ=255)
                                                                                                                        ttGTACTTGAGCGGGTGGCCGAGCAACTGCGGGTGCTCGGCGATGGCCAGGCGCGAGCCCATGTCGGCGTACTTGCCGTAGCTGGCGAAGGCGCCCGACATGGACTTGAGCCCATAGAAGGTCAGCGGTTGGTCGGCGAATGCCCGgtg                           >  2:147998/1‑149 (MQ=255)
                                                                                                                         tGTACTTGAGCGGGTGGCCGAGCAACTGCGGGTGCTCGGCGATGGCCAGGCGCGAGCCCATGTCGGCGTACTTGCCGTAGCTGGCGAAGGCGCCCGACATGGACTTGAGCCCATAGAAGGTCAGCGGTTGGTCGGCGAATGCCCGgt                            <  2:173863/147‑1 (MQ=255)
                                                                                                                          gTACTTGAGCGGGTGGCCGAGCAACTGCGGGTGCTCGGCGATGGCCAGGCGCGAGCCCATGTCGGCGTACTTGCCGTAGCTGGCGAAGGCGCCCGACATGGACTTGAGCCCATAGAAGGTCAGCGGTTGGTCGGCGAATGCCCGGtgtg                         >  4:62545/1‑149 (MQ=255)
                                                                                                                                      gTGGCCGAGCAACTGCGGGTGCTCGGCGATGGCCAGGCGCGAGCCCATGTCGGCGTACTTGCCGTAGCTGGCGAAGGCGCCCGACATGGACTTGAGCCCATAGAAGGTCAGCGGTTGGTCGGCGAATGCCCGGTGTGCCCCCAAGGGGa             <  5:81245/149‑1 (MQ=255)
                                                                                                                                      atcgCCGAGCACCCGCAGTTGCTCGGCAATGGCCAGGCGCGAGCCCATGTCGGCGTACTTGCCGTAGCTGGCGAAGGCGCCCGACATGGACTTGAGCCCATAGAAGGTCAGCGGTTGGTCGGCGAATGCCCGGTGTGCCCCCAAggg               >  6:199081/4‑147 (MQ=255)
                                                                                                                                        ggCCGAGCAACTGCGGGTGCTCGGCGATGGCCAGGCGCGAGCCCATGTCGGCGTACTTGCCGTAGCTGGCGAAGGCGCCCGACATGGACTTGAGCCCATAGAAGGTCAGCGGTTGGTCGGCGAATGCCCGGTGTGCCCCCAAGGGGAgg           <  8:78272/149‑1 (MQ=255)
                                                                                                                                         gCCGAGCAACTGCGGGTGCTCGGCGATGGCCAGGCGCGAGCCCATGTCGGCGTACTTGCCGTAGCTGGCGAAGGCGCCCGACATGGACTTGAGCCCATAGAAGGTCAGCGGTTGGTCGGCGAATGCCCGGTGTGCCCCCAAGGGGAGGc          <  6:110640/149‑1 (MQ=255)
                                                                                                                                          ccGAGCAACTGCGGGTGCTCGGCGATGGCCAGGCGCGAGCCCATGTCGGCGTACTTGCCGTAGCTGGCGAAGGCGCCCGACATGGACTTGAGCCCATAGAAGGTCAGCGGTTGGTCGGCGAATGCCCGGTGTGCCCCCAAGGGGAGGc          >  8:148123/1‑148 (MQ=255)
                                                                                                                                            gAGCACCCGCAGTTGCTCGGCAATGGCCAGGCGCGAGCCCATGTCGGCGTACTTGCCGTAGCTGGCGAAGGCGCCCGACATGGACTTGAGCCCATAGAAGGTCAGCGGTTGGTCGGCGAATGCCCGGTGTGCCCCCAAGGGGAGGCTAc       <  5:199081/149‑1 (MQ=255)
                                                                                                                                            gAGCAACTGCGGGTGCTCGGCGATGGCCAGGCGCGAGCCCATGTCGGCGTACTTGCCGTAGCTGGCGAAGGCGCCCGACATGGACTTGAGCCCATAGAAGGTCAGCGGTTGGTCGGCGAATGCCCGGTGTGCCCCCAAgggggatcgg        >  1:210415/1‑142 (MQ=255)
                                                                                                                                            gAGCAACTGCGGGTGCTCGGCGATGGCCAGGCGCGAGCCCATGTCGGCGTACTTGCCGTAGCTGGCGAAGGCGCCCGACATGGACTTGAGCCCATAGAAGGTCAGCGGTTGGTCGGCGAATGCCCGGTGTGCCCCCAAGGGGAGGCTAc       >  6:142478/1‑149 (MQ=255)
                                                                                                                                               cAACTGCGGGTGTTCGGCGATGGCCAGGCGCGAGCCCATGTCGGCGTACTTGCCGTAGCTGGCGAAGGCGCCCGACATGGACTTGAGCCCATAGAAGGTCAGCGGTTGGTCGGCGAATGCCCGGTGTGCCCCCAAGGGGAGGCTACCt     >  3:12321/1‑148 (MQ=255)
                                                                                                                                                aaCTGCGGGTGCTCGGCGATGGCCAGGCGCGAGCCCATGTCGGCGTACTTGCCGTAGCTGGCGAAGGCGCCCGACATGGACTTGAGCCCATAGAAGGTCAGCGGTTGGTCGGCGAATGCCCGGTGTGCCCCCAAGGGGAGGCTACCTaa   <  6:146153/149‑1 (MQ=255)
                                                                                                                                                 aCTGCGGGTGCTCGGCGATGGCCAGGCGCGAGCCCATGTCGGCGTACTTGCCGTAGCTGGCGAAGGCGCCCGACATGGACTTGAGCCCATAGAAGGTCAGCGGTTGGTCGGCGAATGCCCGGTGTGCCCCCAAGGGGAGGCTACCTAAg  <  2:270328/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
GACACTGCCAGTGCCGAAGACGACAGGGTGCACCCCTGGAAGAAGCGTGCACCATCCTGGCCGATGGCTTCCTGCACCTTGCGCACCGCCTTGCCGGCGTTGCCTTCGGTGTCGATGACTTTGTACTTGAGCGGGTGGCCGAGCAACTGCGGGTGCTCGGCGATGGCCAGGCGCGAGCCCATGTCGGCGTACTTGCCGTAGCTGGCGAAGGCGCCCGACATGGACTTGAGCCCATAGAAGGTCAGCGGTTGGTCGGCGAATGCCCGGTGTGCCCCCAAGGGGAGGCTACCTAAG  >  NC_002947/725561‑725854

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑