Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 81,032 T→A 8.8% intergenic (+89/‑45) tsaC → / → PP_5425 threonylcarbamoyl‑AMP synthase/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00294781,0320TA 8.8% 191.3 / 8.0 68intergenic (+89/‑45)tsaC/PP_5425threonylcarbamoyl‑AMP synthase/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (32/30);  new base A (4/2);  total (36/32)
Fisher's exact test for biased strand distribution p-value = 6.76e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.31e-01

ACCCGAGTGTGATTCGCGACCTGGCAACCGGCGAAGTCGTTCGCCCGGGTTGATACCAATCCACAGGCCTGGCTCGGGACTCGGGCCCCTGTGGGAGCGGGCGCGCCCGCGAAGAATACAACTCGGTGCCAGGCACGGGCTTCGCCCGTGTTCGCGGGCATGCCCGCTCCCGAAAGTGTCACGCACATGACTGCCCTTCGCAGCACGTTTGGTCGCGATGATGGCTATGGTAACGAGGTCTTCGATGAGGCGATTTTTGAACTGACGGCCCGCAAGCCCAAGGCA  >  NC_002947/80891‑81175
                                                                                                                                             |                                                                                                                                               
aCCCGAGTGTGATTCGCGACCTGGCAACCGGCGAAGTCGTTCGCCCGGGTTGATACCAATCCACAGGCCTGGCTCGGGACTCGGGCCCCTGTGGGAGCGGGCGCGCCCGCGAAGAATACAACTCGGTGCCAGGCACGGGCTTCGCCCgt                                                                                                                                          >  4:124582/1‑149 (MQ=255)
    gAGTGTGATTCGCGACCTGGCAACCGGCGAAGTCGTTCGCCCGGGTTGATACCAATCCACAGGCCTGGCTCGGGACTCGGGCCCCTGTGGGAGCGGGCGCGCCCGCGAAGAATACAACTCGGTGCCAGGCACGGGCTTCGCCCGTGTTc                                                                                                                                      <  5:35609/149‑1 (MQ=255)
         tgATTCGCGACCTGGCAACCGGCGAAGTCGTTCGCCCGGGTTGATACCAATCCACAGGCCTGGCTCGGGACTCGGGCCCCTGTGGGAGCGGGCGCGCCCGCGAAGAATACAACTCGGTGCCAGGCACGGGCTTCGCCCGTGTTCGCgg                                                                                                                                  <  3:99522/148‑1 (MQ=255)
            ttCGCGACCTGGCAACCGGCGAAGTCGTTCGCCCAGGTTGATACCAATCCACAGGCCTGGCTCGGGACTCGGGCCCCTGTGGGAGCGGGCGCGCCCGCGAAGAATACAACTCGGTGCCAGGCACGGGCTTCGCCCGTGTTCGCGGGCAt                                                                                                                              >  8:46615/1‑149 (MQ=255)
              cgcgACCTGGCAACCGGCGAAGTCGTTCGCCCGGGTTGATACCAATCCACAGGCCTGGCTCGGGACTCGGGCCCCTGTGGGAGCGGGCGCGCCCGCGAAGAATACAACTCGGTGCCAGGCACGGGCTTCGCCCGTGTTCGCGGGCATGc                                                                                                                            >  7:123393/1‑149 (MQ=255)
                     tGGCAACCGGCGAAGTCGTTCGCCCGGGTTGATACCAATCCACAGGCCTGGCTCGGGACTCGGGCCCCTGTGGGAGCGGGCGCGCCCGCGAAGAATACAACTCGGTGCCAGGCACGGGCTTCGCCCGTGTTCGCGGGCATGCCCGCTcc                                                                                                                     >  7:174298/1‑149 (MQ=255)
                       gCAACCGGCGAAGTCGTTCGCCCGGGTTGATACCAATCCACAGGCCTGGCTCGGGACTCGGGCCCCTGTGGGAGCGGGCGCGCCCGCGAAGAATACAACTCGGTGCCAGGCACGGGCTTCGCCCGTGTTCGCGGGCATGCCCGCTccc                                                                                                                    <  6:65550/148‑1 (MQ=255)
                         aaCCGGCGAAGTCGTTCGCCCGGGTTGATACCAATCCACAGGCCTGGCTCGGGACTCGGGCCCCTGTGGGAGCGGGCGCGCCCGCGAAGAATACAACTCGGTGCCAGGCACGGGCTTCGCCCGTGTTCGCGGGCATGCCCGCTCCCGa                                                                                                                  >  1:201030/1‑148 (MQ=255)
                         aaCCGGCGAAGTCGTTCGCCCGGGTTGATACCAATCCACAGGCCTGGCTCGGGACTCGGGCCCCTGTGGGAGCGGGCGCGCCCGCGAAGAATACAACTCGGTGCCAGGCACGGGCTTCGCCCGTGTTCGCGGGCATGCCCGCTCCCGa                                                                                                                  >  3:207211/1‑148 (MQ=255)
                             ggCGAAGTCGTTCGCCCGGGTTGATACCAATCCACAGGCCTGGCTCGGGACTCGGGCCCCTGTGGGAGCGGGCGCGCCCGCGAAGAATACAACTCGGTGCCAGGCACGGGCGAAGCCCgtgcctg                                                                                                                                     >  4:48985/1‑121 (MQ=255)
                             ggCGAAGTCGTTCGCCCGGGTTGATACCAATCCACAGGCCTGGCTCGGGACTCGGGCCCCTGTGGGAGCGGGCGCGCCCGCGAAGAATACAACTCGGTGCCAGGCACGGGCGAAGCCCgtgcctg                                                                                                                                     <  3:48985/125‑5 (MQ=255)
                                 aaGTCGTTCGCGCGGGTTGATACCTATCCACAGGCCTGGCTCGGGACGCGGGCCCCTGTGGGAGCGGGCGCGCCCGCGAAGAATACAACTCGGTGCCAGGCACGGGCTTCGCCCGTGTTCGCGGGCATGCCCGCTCCCGAAAGTGTCAc                                                                                                         <  7:234731/149‑1 (MQ=255)
                                   gTCGTTCGCCCGGGTTGATACCAATCCACAGGCCTGGCTCGGGACTCGGGCCCCTGTGGGAGCGGGCGCGCCCGCGAAGAATACAACTCGGTGCCAGGCACGGGCTTCGCCCGTGTTCGCGGGCATGCCCGCTCCCGAAAGTGTCACGc                                                                                                       <  8:174298/149‑1 (MQ=255)
                                   gTCGTTCGCCCGGGTTGATACCAATCCACAGGCCTGGCGCGGGACTCGGGCCCCTGTGGGAGCGGGCGCGCCCGCGAAGAATACAACTCGGTGCCAGGCACGGGCTTCGCCCGTGTTCGCGGGCATGCCCGCTCCCGAAAGTGTCACGc                                                                                                       <  8:237125/149‑1 (MQ=255)
                                            ccGGGTTGATACCAATCCACAGGCCTGGCTCGGGACTCGGGCCCCTGTGGGAGCGGGCGCGCCCGCGAAGAATACAACTCGGTGCCAGGCACGGGCTTCGCCCGTGTTCGCGGGCATGccc                                                                                                                          <  5:135445/121‑1 (MQ=255)
                                            ccGGGTTGATACCAATCCACAGGCCTGGCTCGGGACTCGGGCCCCTGTGGGAGCGGGCGCGCCCGCGAAGAATACAACTCGGTGCCAGGCACGGGCTTCGCCCGTGTTCGCGGGCATGccc                                                                                                                          >  6:135445/1‑121 (MQ=255)
                                              gggTTGATACCAATCCACAGGCCTGGATCGGGACTCGGGCCCCTGTGGGAGCGGGCGCGCCCGCGAAGAATACAACTCGGTGCCAGGCACGGGCTTCGCCCGTGTTCGCGGGCATGCCCGCTCCCGAAAGTGTCACGCACATGACTGcc                                                                                            <  3:149406/149‑1 (MQ=255)
                                                    aTACCAATCCACAGGCCTGGCTCGGGACTCGGGCCCCTGTGGGAGCGGGCGCGCCCGCGAAGAATACAACTCGGTGCCAGGCACGGGCTTCGCCCGTGTTCGCGGGCATGc                                                                                                                            <  2:6088/111‑1 (MQ=255)
                                                    aTACCAATCCACAGGCCTGGCTCGGGACTCGGGCCCCTGTGGGAGCGGGCGCGCCCGCGAAGAATACAACTCGGTGCCAGGCACGGGCTTCGCCCGTGTTCGCGGGCATGc                                                                                                                            >  1:6088/1‑111 (MQ=255)
                                                    aTACCAATCCACAGGCCTGGCTCGGGACTCGGGCCCCTGTGGGAGCGGGCGCGCCCGCGAAGAATACAACTCGGTGCCAGGCACGGGCTTCGCCCGTGTTCGCGGGCATGc                                                                                                                            >  5:159339/1‑111 (MQ=255)
                                                    aTACCAATCCACAGGCCTGGCTCGGGACTCGGGCCCCTGTGGGAGCGGGCGCGCCCGCGAAGAATACAACTCGGTGCCAGGCACGGGCTTCGCCCGTGTTCGCGGGCATGc                                                                                                                            >  5:155656/1‑111 (MQ=255)
                                                    aTACCAATCCACAGGCCTGGCTCGGGACTCGGGCCCCTGTGGGAGCGGGCGCGCCCGCGAAGAATACAACTCGGTGCCAGGCACGGGCTTCGCCCGTGTTCGCGGGCATGc                                                                                                                            <  6:155656/111‑1 (MQ=255)
                                                    aTACCAATCCACAGGCCTGGCTCGGGACTCGGGCCCCTGTGGGAGCGGGCGCGCCCGCGAAGAATACAACTCGGTGCCAGGCACGGGCTTCGCCCGTGTTCGCGGGCATGc                                                                                                                            <  6:159339/111‑1 (MQ=255)
                                                        cagtCCACAGGCCTGGCTCGGGACTCGGGCCCCTGTGGGAGCGGGCGCGCCCGCGAAGAATACAACTCGGTGCCAGGCACGGGCTTCGCCCGTGTTCGCGGGCATGCCCGCTCCCGAAAGTGTCACGCACATGACTGCCCTTCgccgca                                                                                  >  1:96970/4‑149 (MQ=255)
                                                        cAATCCACAGGCCTGGCTCGGGACTCGGGCCCCTGTGGGAGCGGGCGCGCCCGCGAAGAATACAACTCGGTGCCAGGCACGGGCTTCGCCCGTGTTCGCGGGCATGCCCGCTCCCGAAAGTGTCACGCACATGACTGCCCTTCgcagca                                                                                  >  5:199273/1‑149 (MQ=255)
                                                        cAATCCACAGGCCTGGCTCGGGACTCGGGCCCCTGTGGGAGCGGGCGCGCCCGCGAAGAATACAACTCGGTGCCAGGCACGGGCTTCGCCCGTGTTCGCGGGCATGCCCGCTCCCGAAAGTGTCACGCACATGACTGCCCTTCgcagca                                                                                  >  8:218674/1‑149 (MQ=255)
                                                        cAATCCACAGGCCTGGCTCGGGACTCGGGCCCCTGTGGGAGCGGGCGCGCCCGCGAAGAATACAACTCGGTGCCAGGCACGGGCTTCGCCCGTGTTCGCGGGCATGCCCGCTCCCGAAAGTGTCACGCACATGACTGCCCTTCgcagca                                                                                  >  7:67267/1‑149 (MQ=255)
                                                             cacaGGCCTGGCTCGGGACTCGGGCCCGTGTGGGAGGGGGCGCGCCCGCGAATAATACAACTCGGTGCCAGGCACGGGCTTCGCCCGTGTTCGCGGGCATGCCCGCTCCCGAAAGTGTCACGCACATGACTGCCCTTCGCAGCACGtt                                                                              <  8:127222/148‑1 (MQ=255)
                                                             cacaGGCCTGGCTCGGGACTCGGGCCCCTGTGGGAGCGGGCGCGCCCGCGAAGAATACAACTCGGTGCCAGGCACGGGCTTCGCCCGTGTTCGCGGGCATGCCCGCTCCCGAAAGTGTCACGCACATGACTGCCCTTCGCAGCACGttt                                                                             <  7:46615/149‑1 (MQ=255)
                                                              acaGGCCTGGCTCGGGACTCGGGCCCCTGTGGGAGCGGGCGCGCCCGCGAAGAATACAACTCGGTGCCAGGCACGGGCTTCGCCCGTGTTCGCGGGCATGCCCGCTCCCGAAAGTGTCACGCACATGACTGCCCTTCGCAGCACGTTTg                                                                            <  8:123393/149‑1 (MQ=255)
                                                                aGGCCTGGCTCGGGACTCGGGCCCCTGTGGGAGCGGGCGCGCCCGCGAAGAATACAACTCGGTGCCAGGCACGGGCTTCGCCCGTGTTCGCGGGCATGCCCGCTCCCGAAAGTGTCACGCACATGACTGCCCTTCGCAGCACGTTTGGt                                                                          <  7:218674/149‑1 (MQ=255)
                                                                    cTGGCTCGGGACTCGGGCCCCTGTGGGAGCGGGCGCGCCCGCGAAGAATACAACTCGGTGCCAGGCACGGGCTTCGCCCGTGTTCGCGGGc                                                                                                                                >  8:50027/1‑91 (MQ=255)
                                                                    cTGGCTCGGGACTCGGGCCCCTGTGGGAGCGGGCGCGCCCGCGAAGAATACAACTCGGTGCCAGGCACGGGCTTCGCCCGTGTTCGCGGGc                                                                                                                                <  7:50027/91‑1 (MQ=255)
                                                                    cTGGCTCGGGACTCGGGCCCCTGTGGGAGCGGGCGCGCCCGCGAAGAATACAACTCGGTGCCAGGCACGGGCTTCGCCCGTGTTCGCGGGCATGCCCGCTCCCGAAAGTGTCACGCACATGACTGCCCTTCGCAGCACGTTTGGTcgc                                                                       >  6:111923/1‑148 (MQ=255)
                                                                    cTGGCTCGGGACTCGGGCCCCTGTGGGAGCGGGCGCGCCCGCGAAGAATACAACTCGGTGCCAGGCACGGGCTTCGCCCGTGTTCGCGGGCATGCCCGCTCCCGAAAGTGTCACGCACATGACTGCCCTTCGCAGCACGTTTGGTcgc                                                                       >  4:256217/1‑148 (MQ=255)
                                                                       gCTCGGGACTCGGGCCCCTGTGGGAGCGGGCGCGCCCGCGAAGAATACAACTCGGTGCCAGGCACGGGCTTCGc                                                                                                                                              >  2:136123/1‑74 (MQ=255)
                                                                       gCTCGGGACTCGGGCCCCTGTGGGAGCGGGCGCGCCCGCGAAGAATACAACTCGGTGCCAGGCACGGGCTTCGc                                                                                                                                              <  1:136123/74‑1 (MQ=255)
                                                                           gggACTCGGGCCCCTGTGGGAGCGGGCGCGCCCGCGAAGAATACAACTCGGTGCCAGGCACGCGCTTCGCCCGTGTTCGCGCGCATGCCCGCTCCCGAAAGTGTCACGCACATGACTGCCCTTCGCAGCACGTTTGGTCGCGATGATgg                                                               >  4:219145/1‑149 (MQ=255)
                                                                              aCTCGGGCCCCTGTGGGAGCGGGCGCGCCCGCGAAGAATACAACTCGGTGCCAGGCACGGGCTTCGCCCGTGTTCGCGGGCATGCCCGCTCCCGaaatt                                                                                                              >  2:221553/1‑97 (MQ=255)
                                                                               cTCGGGCCCCTGTGGGAGCGGGCGCGCCCGCGAAGAATACAACTCGGTGCCAGGCACGGGCTTCGCCCGTGTTCGCGGGCATGCCCGCTCCCGAAAGTGTCACGCACATGACTGCCCTTCGCAGCACGTTTGGTCGCGATGATGGCTAt                                                           <  4:60230/149‑1 (MQ=255)
                                                                                tCGGGCCCCTGTGGGAGCGGGCGCGCCCGCGAAGAATACAACTCGGTGCCAGGCACGGGCTTCGCCCGTGTTCGCGGGCATGCCCGCTCCCGAAAGTGTCACGCACATGACTGCCCTTCGCAGCACGTTTGGTCGCGATGATGGCTATg                                                          >  2:78109/1‑149 (MQ=255)
                                                                                  gggCCCCTGTGGGAGCGGGCGCGCCCGCGAAGAATACAACTCGGTGCCAGGCACGGGCTTCGCCCGTGTTCGCGGGCATGCCCGCTCCCGAAAGTGTCCCGCACATGACTGCCCTTCGCAGCACGTTTGGTCGCGATGATGGCTATgg                                                         >  3:128868/1‑148 (MQ=255)
                                                                                  gggCCCCTGTGGGAGCGGGCGCGCCCGCGAAGAATACAACTCGGTGCCAGGCACGGGCTTCGCCCGTGTTCGCGGGCATGCCCGCTCCCGAAAGTGTCACGCACATGACTGCCCTTCGCAGCACGTTTGGTCGCGATGATGGCTATGGt                                                        <  3:256217/149‑1 (MQ=255)
                                                                                   ggCCCCTGTGGGAGCGGGCGCGCCCGCGAAGAATACAACTCGGTGCCAGGCACGGGCTTCGCCCGTGTTCGCGGGCATGCCCGCTccc                                                                                                                    >  3:263543/1‑88 (MQ=255)
                                                                                   ggCCCCTGTGGGAGCGGGCGCGCCCGCGAAGAATACAACTCGGTGCCAGGCACGGGCTTCGCCCGTGTTCGCGGGCATGCCCGCTccc                                                                                                                    <  4:263543/88‑1 (MQ=255)
                                                                                        cTGTGGGAGCGGGCGCGCCCGCGAAGAATACAACTCGGTGCCAGGCACGGGCTTCGCCCGTGTTCGCGGGCATGCCCGCTCCCGAAAGTGTCACGCACATGACTGCCCTTCGCAGCACGTTTGGTCGCGATGATGGCTATGGTAACGAg                                                  <  1:231856/149‑1 (MQ=255)
                                                                                              gAGCGGGCGCGCCCGCGAAGAATACAACTCGGTGCCAGGCACGGGCTTCGCCCGTGTTCGCGGGCATGCCCGCTCCCGAAAGTGTCACGCACATGACTGCCCTTCGCAGCACGTTTGGTCGCGATGATGGCTATGGTAACGAGGTCTTc                                            >  4:198507/1‑149 (MQ=255)
                                                                                                gCGGGCGCGCCCGCGAAGAATACAACTCGGTGCCAGGCACGGGCTTCGCCCGTGTTCGCGGGCATGCCCGCTCCCGAAAGTGTCACGcac                                                                                                     >  4:162489/1‑90 (MQ=255)
                                                                                                gCGGGCGCGCCCGCGAAGAATACAACTCGGTGCCAGGCACGGGCTTCGCCCGTGTTCGCGGGCATGCCCGCTCCCGAAAGTGTCACGcac                                                                                                     <  3:162489/90‑1 (MQ=255)
                                                                                                   ggCGCGCCCGCGAAGAATACAACTCGGTGCCAGGCACGGGCTTCGCCCGTGTTCGCGGGCATGCCCGCTCCCGAAAGTGTCACGCACATGACTGCCCTTCGCAGCACGTTTGGTCGCGATGATGGCTATGGTAACGAGGTCTTCGATGa                                       <  1:78109/149‑1 (MQ=255)
                                                                                                            gcgAAGAATACAACTCGGTGCCAGGCACGGGCTTCGCCCGTGTTCGCGGGCATGCCCGCTCCCGAAAGTGTCACGCACATGACTGCCCTTCGCAGCACGTTTGGTCGCGATGATGGCTATGGTAACGAGGTCTTCGATGAGGCGAtttt                              <  3:198507/149‑1 (MQ=255)
                                                                                                                agaaTACAACTCGGTGCCAGGCACGGGCTTCGCCCGTGTTCGCGGGCATGCCCGCTCCCGAAAGTGTCACGCACATGACTGCCCTTCGCAGCACGTTTGGTCGCGATGATGGCTATGGTAACGAGGTCTTCGATGAGGCGATTTTTGaa                          <  5:111923/149‑1 (MQ=255)
                                                                                                                    tACAACTCGGTGCCAGGCACGGGCTTCGCCCGTGTTCGCGGGCATGCCCGCTCCCGAAAGTGTCACGCACATGACTGCCCTTCGCAGCACGTTTGGTCGCGATGATGGCTATGGTAACGAGGTCTTCGATGAGGCGATTTTTGAACTGa                      <  8:67267/149‑1 (MQ=255)
                                                                                                                    tACAACTCGGTGCCAGGCACGGGCTTCGCCCGTGTTCGCGGGCATGCCCGCTCCCGAAAGTGTCACGCACATGACTGCCCTTCGCAGCACGTTTGGTCGCGATGATGGCTATGGTAACGAGGTCTTCGATGAGGCGATTTTTGAACTGa                      >  1:146408/1‑149 (MQ=255)
                                                                                                                    tACAACTCGGTGCCAGGCACGGGCTTCGCCCGTGTTCGCGGGCATGCCCGCTCCCGAAAGTGTCACGCACATGACTGCCCTTCGCAGCACGTTTGGTCGCGATGATGGCTATGGTAACGAGGTCTTCGATGAGGCGATTTTTGAACTGa                      <  6:199273/149‑1 (MQ=255)
                                                                                                                       aaCTCGGTGCCAGGCACGGGCTTCGCCCGTGTTCGCGGGCATGCCCGCTCCCGAAAGTGTCACGCACATGACTGCCCTTCGCAGCACGTTTGGTCGCGATGATGGCTATGGTAACGAGGTCTTCGATGAGGCGATTTTTGAACTGACgg                   >  2:216704/1‑149 (MQ=255)
                                                                                                                        aCTCGGTGCCAGGCACGGGCTTCGCCCGTGTTCGCGGGCATGCCCGCTCCCGAAAGTGTCACGCACATGACTGCCCTTCGCAGCACGTTTGGTCGCGATGATGGCTATGGTAACGAGGTCTTCGATGAGGCGATTTTTGAACTGACGGc                  >  5:12521/1‑149 (MQ=255)
                                                                                                                         cTCGGTGCCAGGCACGGCCTTCGCCCGTGTTCGCGGGCAAGCCCGCTACCGAAAGTGTCACGCACATGACTGCCCTTCGCAGCACGTTTGGTCGCGATGATGGCTATGGTAACGAGGTCTTCGATGAGGCGATTTTTGAACTGACGGcc                 >  2:127814/1‑149 (MQ=255)
                                                                                                                            ggTGCCAGGCACGGGCTTCGCCCGTGTTCGCGGGCATGCCCGCTCCCGAAAGTGTCACGCACATGACTGCCCTTCGCAGCACGTTTGGTCGCGATGATGGCTATGGTAACGAGGTCTTCGATGAGGCGATTTTTGAACTGACGGCCCGc              <  6:12521/149‑1 (MQ=255)
                                                                                                                              tGCCAGGCACGGGCTTCGCCCGTGTTCGCGGGCATGCCCGCTCCCGAAAGTGTCACGCACATGACTGCCCTTCGCAGCACGGTTGGTCGCGATGATGGCTATGGTAACGAGGTCTTCGATGAGGCGATTTTTGAACTGACGGCCCGc              >  7:211437/1‑147 (MQ=255)
                                                                                                                               cgcgaacACGGGCGAAGCCCGTGTTCGCGGGCATGCCCGCTCCCGAAAGTGTCACGCACATGACTGCCCTTCGCAGCACGTTTGGTCGCGATGATGGCTATGGTAACGAGGTCTTCGATGAGGCGATTTTTGAACTGACGGCCCGCAAg           >  6:128601/7‑149 (MQ=255)
                                                                                                                                  gaacACGGGCGAAGCCCGTGTTCGCGGGCATGCCCGCTCCCGAAAGTGTCACGCACATGACTGCCCTTCGCAGCACGTTTGGTCGCGATGATGGCTATGGTAACGAGGTCTTCGATGAGGCGATTTTTGAACTGACGGCCCGCAAGcc         >  1:137271/4‑148 (MQ=255)
                                                                                                                                    acACGGGCGAAGCCCGTGTTCGCGGGCATGCCCGCTCCCGAAAGTGTCACGCACATGACTGCCCTTCGCAGCACGTTTGGTCGCGATGATGGCTATGGTAACGAGGTCTTCGATg                                        >  7:89376/2‑115 (MQ=255)
                                                                                                                                    acACGGGCGAAGCCCGTGTTCGCGGGCATGCCCGCTCCCGAAAGTGTCACGCACATGACTGCCCTTCGCAGCACGTTTGGTCGCGATGATGGCTATGGTAACGAGGTCTTCGATg                                        <  8:89376/114‑1 (MQ=255)
                                                                                                                                     cACGGGCTTCGCCCGTGTTCGCGGGCATGCCCGCTCCCGAAAGTGTCACGCCCATGACTGCCCTTCGCAGCACGTTTGGTCGCGAAGATGGCTATGGTAACGAGGTCTTCGATGAGGCGATTTTTGAACTGACGGCCCGCAAGCCCAAg     <  4:207211/149‑1 (MQ=255)
                                                                                                                                        gggCTTCGCCCGTGTTCGCGGGCATGCCCGCTCCCGAAAGTGTCACGCACATGACTGCCCTTCGCAGCACGTTTGGTCGCGATGATGGCTATGGTAACGAGGTCTTCGATGAGGCGATTTTTGAACTGACGGccc                <  6:227580/135‑1 (MQ=255)
                                                                                                                                        gggCTTCGCCCGTGTTCGCGGGCATGCCCGCTCCCGAAAGTGTCACGCACATGACTGCCCTTCGCAGCACGTTTGGTCGCGATGATGGCTATGGTAACGAGGTCTTCGATGAGGCGATTTTTGAACTGACGGccc                >  5:227580/1‑135 (MQ=255)
                                                                                                                                          gCTTCGCCCGTGTTCGCGGGCATGCCCGCTCCCGAAAGTGTCACGCACATGACTGCCCTTCGCAGCACGTTTGGTCGCGATGATGGCTATGGTAACGAGGTCTTCGATGAGGCGATTTTTGAACTGACGGCCCGCAAGCCCAAGGCa  >  1:266494/1‑147 (MQ=255)
                                                                                                                                             |                                                                                                                                               
ACCCGAGTGTGATTCGCGACCTGGCAACCGGCGAAGTCGTTCGCCCGGGTTGATACCAATCCACAGGCCTGGCTCGGGACTCGGGCCCCTGTGGGAGCGGGCGCGCCCGCGAAGAATACAACTCGGTGCCAGGCACGGGCTTCGCCCGTGTTCGCGGGCATGCCCGCTCCCGAAAGTGTCACGCACATGACTGCCCTTCGCAGCACGTTTGGTCGCGATGATGGCTATGGTAACGAGGTCTTCGATGAGGCGATTTTTGAACTGACGGCCCGCAAGCCCAAGGCA  >  NC_002947/80891‑81175

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑