Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	743,430	G→A	8.3%	intergenic (+109/+165)	PP_0635 → / ← PP_0636	group II intron‑encoding maturase/cold shock DNA‑binding domain‑containing protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	743,430	0	G	A	8.3%	135.6 / 2.5	48	intergenic (+109/+165)	PP_0635/PP_0636	group II intron‑encoding maturase/cold shock DNA‑binding domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base G (23/21);  new base A (2/2);  total (25/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.93e-01

GATACTGGATACGATTAACCGGCTTAGCCGCGTAACCTGAACCGCCGTGTACGGAACCGTACGCACGGTGGTGTGAGAGGACGGCGGGTGTGAATCCGCCTCCTACTCGATTGCGCGAAATCAGACCTGCCTTGTGTGCCCGCTATGTGAAGCAACCTTCGGTTGCGTTACATAGCGGACACACAGCGTCGTGACTTGCCGGGGAAACTACTTGGTCGCGGCGTGTGGAGCAGAGACTGTAGGGGAGCGTTGACAGTGGCGGTTTCAAGCTAGCTTGGCGGTCACGCTAAGCCGAG  >  NC_002947/743282‑743577                                                                                                                                                     |                                                                                                                                                    gATACTGGATACGATTAACCGGCTTAGCCGCGTAACCTGAACCGCCTTGTACGGAACCGTACGCACGGTGGTGTGAGAGGACGGCGGGTGTGAATCCGCCTCCTACTCGATTGCGCGAAATCAGACCTGCCTTGTGTGCCCGCTAtgtg                                                                                                                                                     <  1:256752/149‑1 (MQ=255)           aCGATTAACCGGCTTAGCCGCGTAACCTAAACCGCCGTGTACGGAACCGTACGCACGGTGGTGTGAGAGGACGGCGGGTGTGAATCCGCCTCCTACTCGATTGCGCGAAATCAGACCTGCCTTGTGTGCCCGCTATGTGAAGCAACCtt                                                                                                                                           >  1:152259/1‑149 (MQ=255)              aTTAACCGGCTTAGCCGCGTAACCTGAACCGCCGTGTACGGAACCGTACGCACGGTGGTGTGAGAGGACGGCGGGTGTGAATCCGCCTCCTACTCGATTGCGCGAAATCAGACCTGCCTTGTGTGCCCGCTATGTGAAGCAACCTTCgg                                                                                                                                        >  3:233110/1‑149 (MQ=255)                    cGGCTTAGCCGCGTAACCTGAACCGCCGTGTACGGAACCGTACGCACGGTGGTGTGAGAGGACGGCGGGTGTGAATCCGCCTCCTACTCGATTGCGCGAAATCAGACCTGCCTTGTGTGCCCGCTATGTGAAGCAACCTTCGGTTGCGt                                                                                                                                  >  8:221371/1‑149 (MQ=255)                     ggCTTAGCCGCGTAACCTGAACCGCCGTGTACGGAACCGTACGCACGGTGGTGTGAGAGGACGGCGGGTGTGAATCCGCCTCCTACTCGATTGCGCGAAATCAGACCTGCCTTGTGTGCCCGCTATGTGAAGCAACCTTCGGTTGCGtt                                                                                                                                 <  3:223035/149‑1 (MQ=255)                      gCTTAGCCGCGTAACCTGAACCGCCGTGTACGGAACCGTACGCACGGTGGTGTGAGAGGACGGCGGGTGTGAATCCGCCTCCTACTCGATTGCGCGAAATCAGACCTGCCTTGTGTGCCCGCTATGTGAAGCAACCTTCGGTTGCGTTa                                                                                                                                <  2:152259/149‑1 (MQ=255)                                   aCCTGAACCGCCGTGTACGGAACCGTACGCACGGTGGTGTGAGAGGACGGCGGGTGTGAATCCGCCTCCTACTCGATTGCGCGAAATCAGACCTGCCTTGTGTGCCCGCTATGTGAAGCAACCTTCGGTTGCGTTACATAGCGGacaca                                                                                                                   >  7:229505/1‑149 (MQ=255)                                     cTGAACCGCCGTGTACGGAACCGTACGCACGGTGGTGTGAGAGGACGGCGGGTGTGAATCCGCCTCCTACTCGATTGCGCGAAATCAGACCTGCCTTGTGTGCCCGCTATGTGAAGCAACCTTCGGTTGCGTTACATAGCGGacaca                                                                                                                   >  6:8675/1‑147 (MQ=255)                                        aaCCGCCGTGTACGGAACCGTACGCACGGTGGTGTGAGAGGACGGCGGGTGTGAATCCGCCTCCTACTCGATTGCGCGAAATCAGACCTGCCTTGTGTGTCCGCTATGTAACGCAACCGAAGGTTGCTTCACATAGCGGGcacacaagg                                                                                                              <  1:79077/149‑4 (MQ=18)                                           cgccgTGTACGGAACCGTACGCACGGTGGTGTGAGAGGACGGCGGGTGTGAATCCGCCTCCTACTCGATTGCGCGAAATCAGACCTGCCTTGTGTGCCCGCTATGTGAAGCAACCTTCGGTTGCGTTACATAGCGGACACACAGcgtcg                                                                                                           <  8:229505/149‑1 (MQ=255)                                           cgccgTGTACGGAACCGTACGCACGGGGGTGTGAGAGGACGGCGGGTGTGAATCCGCCTCGTACTCGATTGCGCGAATTCAGACCTGCCTTGTGTGCCCGCTATGTGAAGCAACCTTCGGGTGCGTTTCAGAGCGGGGACACAGcgggg                                                                                                           >  8:94320/1‑146 (MQ=21)                                            gccgTGTACGGAACCGTACGCACGGTGGTGTGAGAGGACGGCGGGTGTGAATCCGCCTCCTACTCGATTGCGCGAAATCAGACCTGCCTTGTGTGCCCGCTATGTGAAGCAACCTTCGGTTGCGTTACATAGCGGACACACAGcgtcgt                                                                                                          <  3:191146/149‑1 (MQ=255)                                             ccgTGTACGGAACCGTACGCACGGTGGTGTGAGAGGACGGCGGGTGTGAATCCGCCTCCTACTCGATTGCGCGAAATCAGACCTGCCTTGTGTGCCCGCTATGTGAAGCAACCTTCGGTTGCGTTACATAGCGGACACACAGcgtcgt                                                                                                          >  4:34938/1‑148 (MQ=255)                                                     ggAACCGTACGCACGGTGGTGTGAGAGGACGGCGGGTGTGAATCCGCCTCCTACTCGATTGCGCGAAATCAGACCTGCCTTGTGTGCCCGCTATGTGAAGCAACCTTCGGTTGCGTTACATAGCGGACACACAGCGTCGTGACTTGCCg                                                                                                 <  3:34938/149‑1 (MQ=255)                                                         ccGTACGCACGGTGGTGTGAGAGGACGGCGGGTGTGAATCCGCCTCCTACTCGATTGCGCGAAATCAGACCTGCCTTGTGTGCCCGCTATGTGAAGCAACCTTCGGTTGCGTTACATAGCGGACACACAGCGTCGTGACTTGCCgggg                                                                                              >  6:251011/1‑148 (MQ=255)                                                         ccGTACGCACGGTGGTGTGAGAGGACGGCGGGTGTGAATCCGCCTCCTACTCGATTGCGCGAAATCAGACCTGCCTTGTGTGACCGCAATGTGAATCAACCATCGGTTGCGTTACAAAGACGACACACAACGAAGTTACATTCCgggg                                                                                              >  6:39284/1‑148 (MQ=21)                                                               gCACGGTGGTGTGAGAGGACGGCGGGTGTGAATCCGCCTCCTACTCGATTGCGCGAAATCAGACCTGCCTTGTGTGCCCGCTATGTGAAGCAACCTTCGGTTGCGTTACATAGCGGACACACAGCGTCGTGACTTGCCGGGGAAactac                                                                                       >  6:217303/1‑149 (MQ=255)                                                                 aCGGTGGTGTGAGAGGACGGCGGGTGTGAATCCGCCTCCTACTCGATTGCGCGAAATCAGACCTGCCTTGTGTGCCCGCTATGTGAAGCAACCTTCGGTTGCGTTACATAGCGGACACACAGCGTCGTGACTTGCCGGGGAAACTACtt                                                                                     >  3:108466/1‑149 (MQ=255)                                                                       gtgtGAGAGGACGGCGGGTGTGAATCCGCCTCCTACTCGATTGCGCGAAATCAGACCTGCCTTGTGTGCCCGCTATGTGAAGCAACCTTCGGTTGCGTTACATAGCGGACACACAGCGTCGTGACTTGCCGGGGAAACTACTTGGTcgc                                                                               <  5:217303/149‑1 (MQ=255)                                                                         gtgAGAGGACGGCGGGTGTGTATCCGCCTCCTACTCGATTGCGCGAAATCAGACCTGCCTTGTGTGCCCGCTATGTGAAGCAACCTTCGGTTGCGTTACATAGCGGACACACAGCGTCGTGACTTGCCGGGGAAACTACTTgg                                                                                   <  1:190853/143‑1 (MQ=255)                                                                         gtgAGAGGACGGCGGGTGTGTATCCGCCTCCTACTCGATTGCGCGAAATCAGACCTGCCTTGTGTGCCCGCTATGTGAAGCAACCTTCGGTTGCGTTACATAGCGGACACACAGCGTCGTGACTTGCCGGGGAAACTACTTgg                                                                                   >  2:190853/1‑143 (MQ=255)                                                                          tgAGAGGACGGCGGGTGTGAATCCGCCTCCTACTCGATTGCGCGAAATCAGTCCTGCCTTGTGTGCCCGCTATGTGAAGCAACCTTCGGTTGCGTTACATAGCGGACACACAGcgtcg                                                                                                           <  7:66055/118‑1 (MQ=255)                                                                          tgAGAGGACGGCGGGTGTGAATCCGCCTCCTACTCGATTGCGCGAAATCAGTCCTGCCTTGTGTGCCCGCTATGTGAAGCAACCTTCGGTTGCGTTACATAGCGGACACACAGcgtcg                                                                                                           >  8:66055/1‑118 (MQ=255)                                                                              agGACGGCGGGTGTGAATCCGCCTCCTACTCGATTGCGCGAAATCAGACCTGCCTTGTGTGCCCGCTATGTGAAGCAACCTTCGGTTGCGTTACATAGCGGACACACAGCGTCGTGACTTGCCGGGGAAACTACTTGGTCGCGGCgtgt                                                                        <  5:8675/149‑1 (MQ=255)                                                                               ggACGGCGGGTGTGAATCCGCCTCCTACTCGATTGCGCGAAATCAGACCTGCCTTGTGTGCCCGCTATGTGAAGCAACCTTCGGTTGCGTTACATAGCGGACACACAGCGTCGTGACTTGCCGGGGAAACTACTTGGTCgcggc                                                                            <  2:39823/144‑1 (MQ=255)                                                                               ggACGGCGGGTGTGAATCCGCCTCCTACTCGATTGCGCGAAATCAGACCTGCCTTGTGTGCCCGCTATGTGAAGCAACCTTCGGTTGCGTTACATAGCGGACACACAGCGTCGTGACTTGCCGGGGAAACTACTTGGTCgcggc                                                                            >  1:39823/1‑144 (MQ=255)                                                                               ggACGGCGGGTGTGAATCCGCCTCCTACTCGATTGCGCGAAATCAGACCTGCCTTGTGTGCCCGCTATGTGAAGCAACCTTCGGTTGCGTTACATAGCGGACACACAGCGTCGTGACTTGCCGGGGAAACTACTTGGTCGCGGCGtgtg                                                                       >  3:10275/1‑149 (MQ=255)                                                                                 aCGGCGGGTGTGAATCCGCCTCCTACTCGATTGCGCGAAATCAGACCTGCCTTGTGTGCCCGCTATGTGAAGCAACCTTCGGTTGCGTTACATAGCGGACACACAGCGTCGTGACTTGCCGGGGAAACTACTTGGTCGCGGCGTGTGGa                                                                     >  5:185480/1‑149 (MQ=255)                                                                                 aCGGCGGGTGTGAATCCGCCTCCTACTCGATTGCGCGAAATCAGACCTGCCTTGTGTGCCCGCTATGTGAAGCAACCTTCGGTTGCGTTACATAGCGGACACACAGCGTCGTGACTTGCCGGGGAAACTACTTGGTCGCGGCGTGTGGa                                                                     <  4:233110/149‑1 (MQ=255)                                                                                           tgAATCCGCCTCCTACTCGATTGCGCGAAATCAGACCTGCCTTGTGTGCCCGCTATGTGAAGCAACCTTCGGTTGCGTTACATAGCGGACACACAGCGTCGTGACTTGCCGGGGAAACTACTTGGTCGCGGCGTGTGGAGCAGAGACt                                                            <  4:10275/148‑1 (MQ=255)                                                                                            gAATCCGCCTCCTACTCGATTGCGCGAAATCAGACCTGCCTTGTGTGCCCGCTATGTGAAGCAACCTTCGGTTGCGTTACATAGCGGACACACAGCGTCGTGACTTGCCGGGGAAACTACTTGGTCGCGGCGTGTGGAGCAGAGACTg                                                           >  5:161901/1‑148 (MQ=255)                                                                                               tCCGCCTCCTACTCGATTGCGCGAAATCAGACCTGCCTTGTGTGCCCGGTATGTGAAGCAACCTTCGGTTGTGTTACATAGCGGACACACAGCGTCGTGACTTGCCGGGGAAACTACTTGGTCGCGGCGTGTGGAGCAGAGACTGTAgg                                                       <  8:96316/149‑1 (MQ=255)                                                                                                 cGCCTCCTACTCGATTGCGCGAAATCAGACCTGCCTTGTGTGCCCGCTATGTGAAGCAACCTTCGGTTGCGTTACATAGCGGACACACAGCGTCGTGACTTGCCGGGGAAACTACTTGGTCGCGGCGTGTGGAGCAGAGACTGTAggg                                                      <  8:84294/148‑1 (MQ=255)                                                                                                       ctACTCGATTGCGCGAAATCAGACCTGCCTTGTGTGCCCGCTATGTGAAGCAACCTTCGGTTGCGTTACATAGCGGACACACAGCGTCGTGACTTGCCGGGGAAACTACTTGGTCGCGGCGTGTGGAGCAGAGACTGTAGGGGAGCg                                                 <  7:221371/147‑1 (MQ=255)                                                                                                         aCTCGATTGCGCGAAATCAGACCTGCCTTGTGTGCCCGCTATGTGAAGCAACCTTCGGTTGCGTTACATAGCGGACACACAGCGTCGTGACTTGCCGGGGAAACTACTTGGTCGCGGCGTGTGGAGCAGAGACTGTAGGGGAGCg                                                 >  8:229502/1‑145 (MQ=255)                                                                                                         aCTCGATTGCGCGAAATCAGACCTGCCTTGTGTGCCCGCTATGTGAAGCAACCTTCGGTTGCGTTACATAGCGGACACACAGCGTCGTGACTTGCCGGGGAAACTACTTGGTCGCGGCGTGTGGAGCAGAGACTGTAGGGGAGCg                                                 <  7:229502/145‑1 (MQ=255)                                                                                                             gATTGCGCGAAATCAGACCTGCCTTGTGTGCCCGCTATGTGAAGCAACCTTCGGTTGCGTTACATAGCGGACACACAGCGTCGTGACTTGCCGGGGAAACTACTTGGTCGCGGCGTGTGGAGCAGAGACTGTAGGGGAGCGTTGACAgg                                         >  2:190366/1‑148 (MQ=255)                                                                                                                     gAAATCAGACCTGCCTTGTGTGCCCGCTATGTGAAGCAACCTTCGGTTGCGTTACATAGCGGACACACAGCGTCGTGACTTGCCGGGGAAACTACTTGGTCGCGGCGTGTGGAGCAGAGACTGTAggg                                                      >  6:176877/1‑128 (MQ=255)                                                                                                                     gAAATCAGACCTGCCTTGTGTGCCCGCTATGTGAAGCAACCTTCGGTTGCGTTACATAGCGGACACACAGCGTCGTGACTTGCCGGGGAAACTACTTGGTCGCGGCGTGTGGAGCAGAGACTGTAggg                                                      <  5:176877/128‑1 (MQ=255)                                                                                                                                 gCCTTGTGTGCCCGCTATGTGAAGCAACCTTCGGTTGCGTTAGATAGCGGACACACAGCGTCGTGACTTGCCGGGGCAACTACTTGGTCGCGGCGTGTGGAGCAGAGACTGTAGGGGAGCGTTGACAGTGGCGGt                                   >  3:162854/1‑135 (MQ=255)                                                                                                                                 gCCTTCTGTGCCCGCTATGTGAAGCAACCTTCGGTTGCGTTACATAGGGGACACACAGCGTCGTGACTTGCCGGGGAAACTACTTGGTCGCGGCGTGTTGAGCAGAGACTGTAGGGGAGCGTTGACAGTGGCGGt                                   <  4:162854/135‑1 (MQ=255)                                                                                                                                  ccTTGTGTGCCCGCTATGTGAAGCAACCTTCGGTTGCGTTACATAGCGGACACACAGCGTCGTGACTTGCCGGTGAAACTACTTGGTCGCGTCGTGTGGAGCAGAGACTGTAGGGGAGCGTTGACAGTGGCGGTTTCAAGCTAGCTTgg                    <  5:39284/149‑1 (MQ=255)                                                                                                                                         tgCCCGCTATGTGAAGCAACCTTCGGTTGCGTTACATAGCGGACACACAGCGTCGTGACTTGCCGGGGAAACTACTTGGTCGCGGCGTGTGGAGCAGAGACTGTAGGGGAGCg                                                 <  6:273500/113‑1 (MQ=255)                                                                                                                                         tgCCCGCTATGTGAAGCAACCTTCGGTTGCGTTACATAGCGGACACACAGCGTCGTGACTTGCCGGGGAAACTACTTGGTCGCGGCGTGTGGAGCAGAGACTGTAGGGGAGCg                                                 >  5:273500/1‑113 (MQ=255)                                                                                                                                             cGCTATGTGAAGCAACCTTCGGTTGCGTTACATAGCGGACACACAGCGTCGTGACTTGCCGGGGAAACTACTTGGTCGCGGCGTGTGGAGCAGAGACTGTAGGGGAGCGTTGACAGTGGCGGTTTCAAGCTAGCTTGGCGGTCACGCt          >  1:264009/1‑148 (MQ=255)                                                                                                                                             cGCTATGTAACGCAACCGAAGGTTGCTTCACATAGCGGGCACACAGCGTCGTGACTTGCCGGGGAAACTACTTGGTCGCGGCGTGTGGAGCAGAGACTGTAGGGGAGCGTTGACAGTGGCGGTTTCAAGCTAGCTTGGCGGTCACGCt          >  7:201726/1‑148 (MQ=37)                                                                                                                                              gCTATGTGAAGCAACCTTCGGTTGCGTTACATAGCGGACACACAGCGTCGTGACTTGCCGGGGAAACTACTTGGTCGCGGCGTGTGGAGCAGAGACTGTAGGGGAGCGTTGACAGTGGCGGTTTCAAGCTAGCTTGGCGGTCACGCTaa        <  6:185480/149‑1 (MQ=255)                                                                                                                                              gCTATGTAACGCAACCGAAGGTTGCTTCACATAGCGGACACACAGCGTCGTGACTTGCCGGGGAAACTACTTGGTCGCGGCGTGTgg                                                                      <  6:83227/87‑1 (MQ=18)                                                                                                                                              gCTATGTAACGCAACCGAAGGTTGCTTCACATAGCGGACACACAGCGTCGTGACTTGCCGGGGAAACTACTTGGTCGCGGCGTGTgg                                                                      >  5:83227/1‑87 (MQ=18)                                                                                                                                                  tgtgAAGCAACCTTCGGTTGCGTTACATAGCGGACACACAGCGTCGTGACTTGCCGGGGAAACTACTTGGTCGCGGCGTGTGGAGCAGAGACTGTAGGGGAGCGTTGACAGTGGCGGTTTCAAGCTAGCTTGGCGGTCACGCTAAGcc     >  4:171927/1‑148 (MQ=255)                                                                                                                                                    tgAAGCAACCTTCGGTTGCGTTACATAGCGGACACACAGCGTCGTGACTTGCCGGGGAAACTACTTGGTCGCGGCGTGTGGAGCAGAGACTGTAGGGGAGCGTTGACAGTGGCGGTTTCAAGCTAGCTTGGCGGTCACGCTAAGCCGAg  <  6:161901/149‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                    GATACTGGATACGATTAACCGGCTTAGCCGCGTAACCTGAACCGCCGTGTACGGAACCGTACGCACGGTGGTGTGAGAGGACGGCGGGTGTGAATCCGCCTCCTACTCGATTGCGCGAAATCAGACCTGCCTTGTGTGCCCGCTATGTGAAGCAACCTTCGGTTGCGTTACATAGCGGACACACAGCGTCGTGACTTGCCGGGGAAACTACTTGGTCGCGGCGTGTGGAGCAGAGACTGTAGGGGAGCGTTGACAGTGGCGGTTTCAAGCTAGCTTGGCGGTCACGCTAAGCCGAG  >  NC_002947/743282‑743577

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑