Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 743,448 G→T 7.4% intergenic (+127/+147) PP_0635 → / ← PP_0636 group II intron‑encoding maturase/cold shock DNA‑binding domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947743,4480GT 7.4% 165.1 / 4.0 54intergenic (+127/+147)PP_0635/PP_0636group II intron‑encoding maturase/cold shock DNA‑binding domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base G (24/26);  new base T (2/2);  total (26/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-02

CGGCTTAGCCGCGTAACCTGAACCGCCGTGTACGGAACCGTACGCACGGTGGTGTGAGAGGACGGCGGGTGTGAATCCGCCTCCTACTCGATTGCGCGAAATCAGACCTGCCTTGTGTGCCCGCTATGTGAAGCAACCTTCGGTTGCGTTACATAGCGGACACACAGCGTCGTGACTTGCCGGGGAAACTACTTGGTCGCGGCGTGTGGAGCAGAGACTGTAGGGGAGCGTTGACAGTGGCGGTTTCAAGCTAGCTTGGCGGTCACGCTAAGCCGAGTGAGGCATTC  >  NC_002947/743301‑743587
                                                                                                                                                   |                                                                                                                                           
cGGCTTAGCCGCGTAACCTGAACCGCCGTGTACGGAACCGTACGCACGGTGGTGTGAGAGGACGGCGGGTGTGAATCCGCCTCCTACTCGATTGCGCGAAATCAGACCTGCCTTGTGTGCCCGCTATGTGAAGCAACCTTCGGTTGCGt                                                                                                                                            >  8:221371/1‑149 (MQ=255)
 ggCTTAGCCGCGTAACCTGAACCGCCGTGTACGGAACCGTACGCACGGTGGTGTGAGAGGACGGCGGGTGTGAATCCGCCTCCTACTCGATTGCGCGAAATCAGACCTGCCTTGTGTGCCCGCTATGTGAAGCAACCTTCGGTTGCGtt                                                                                                                                           <  3:223035/149‑1 (MQ=255)
  gCTTAGCCGCGTAACCTGAACCGCCGTGTACGGAACCGTACGCACGGTGGTGTGAGAGGACGGCGGGTGTGAATCCGCCTCCTACTCGATTGCGCGAAATCAGACCTGCCTTGTGTGCCCGCTATGTGAAGCAACCTTCGGTTGCGTTa                                                                                                                                          <  2:152259/149‑1 (MQ=255)
               aCCTGAACCGCCGTGTACGGAACCGTACGCACGGTGGTGTGAGAGGACGGCGGGTGTGAATCCGCCTCCTACTCGATTGCGCGAAATCAGACCTGCCTTGTGTGCCCGCTATGTGAAGCAACCTTCGGTTGCGTTACATAGCGGacaca                                                                                                                             >  7:229505/1‑149 (MQ=255)
                 cTGAACCGCCGTGTACGGAACCGTACGCACGGTGGTGTGAGAGGACGGCGGGTGTGAATCCGCCTCCTACTCGATTGCGCGAAATCAGACCTGCCTTGTGTGCCCGCTATGTGAAGCAACCTTCGGTTGCGTTACATAGCGGacaca                                                                                                                             >  6:8675/1‑147 (MQ=255)
                    aaCCGCCGTGTACGGAACCGTACGCACGGTGGTGTGAGAGGACGGCGGGTGTGAATCCGCCTCCTACTCGATTGCGCGAAATCAGACCTGCCTTGTGTGTCCGCTATGTAACGCAACCGAAGGTTGCTTCACATAGCGGGcacacaagg                                                                                                                        <  1:79077/149‑4 (MQ=18)
                       cgccgTGTACGGAACCGTACGCACGGTGGTGTGAGAGGACGGCGGGTGTGAATCCGCCTCCTACTCGATTGCGCGAAATCAGACCTGCCTTGTGTGCCCGCTATGTGAAGCAACCTTCGGTTGCGTTACATAGCGGACACACAGcgtcg                                                                                                                     <  8:229505/149‑1 (MQ=255)
                       cgccgTGTACGGAACCGTACGCACGGGGGTGTGAGAGGACGGCGGGTGTGAATCCGCCTCGTACTCGATTGCGCGAATTCAGACCTGCCTTGTGTGCCCGCTATGTGAAGCAACCTTCGGGTGCGTTTCAGAGCGGGGACACAGcgggg                                                                                                                     >  8:94320/1‑146 (MQ=21)
                        gccgTGTACGGAACCGTACGCACGGTGGTGTGAGAGGACGGCGGGTGTGAATCCGCCTCCTACTCGATTGCGCGAAATCAGACCTGCCTTGTGTGCCCGCTATGTGAAGCAACCTTCGGTTGCGTTACATAGCGGACACACAGcgtcgt                                                                                                                    <  3:191146/149‑1 (MQ=255)
                         ccgTGTACGGAACCGTACGCACGGTGGTGTGAGAGGACGGCGGGTGTGAATCCGCCTCCTACTCGATTGCGCGAAATCAGACCTGCCTTGTGTGCCCGCTATGTGAAGCAACCTTCGGTTGCGTTACATAGCGGACACACAGcgtcgt                                                                                                                    >  4:34938/1‑148 (MQ=255)
                                 ggAACCGTACGCACGGTGGTGTGAGAGGACGGCGGGTGTGAATCCGCCTCCTACTCGATTGCGCGAAATCAGACCTGCCTTGTGTGCCCGCTATGTGAAGCAACCTTCGGTTGCGTTACATAGCGGACACACAGCGTCGTGACTTGCCg                                                                                                           <  3:34938/149‑1 (MQ=255)
                                     ccGTACGCACGGTGGTGTGAGAGGACGGCGGGTGTGAATCCGCCTCCTACTCGATTGCGCGAAATCAGACCTGCCTTGTGTGCCCGCTATGTGAAGCAACCTTCGGTTGCGTTACATAGCGGACACACAGCGTCGTGACTTGCCgggg                                                                                                        >  6:251011/1‑148 (MQ=255)
                                     ccGTACGCACGGTGGTGTGAGAGGACGGCGGGTGTGAATCCGCCTCCTACTCGATTGCGCGAAATCAGACCTGCCTTGTGTGACCGCAATGTGAATCAACCATCGGTTGCGTTACAAAGACGACACACAACGAAGTTACATTCCgggg                                                                                                        >  6:39284/1‑148 (MQ=21)
                                           gCACGGTGGTGTGAGAGGACGGCGGGTGTGAATCCGCCTCCTACTCGATTGCGCGAAATCAGACCTGCCTTGTGTGCCCGCTATGTGAAGCAACCTTCGGTTGCGTTACATAGCGGACACACAGCGTCGTGACTTGCCGGGGAAactac                                                                                                 >  6:217303/1‑149 (MQ=255)
                                             aCGGTGGTGTGAGAGGACGGCGGGTGTGAATCCGCCTCCTACTCGATTGCGCGAAATCAGACCTGCCTTGTGTGCCCGCTATGTGAAGCAACCTTCGGTTGCGTTACATAGCGGACACACAGCGTCGTGACTTGCCGGGGAAACTACtt                                                                                               >  3:108466/1‑149 (MQ=255)
                                                   gtgtGAGAGGACGGCGGGTGTGAATCCGCCTCCTACTCGATTGCGCGAAATCAGACCTGCCTTGTGTGCCCGCTATGTGAAGCAACCTTCGGTTGCGTTACATAGCGGACACACAGCGTCGTGACTTGCCGGGGAAACTACTTGGTcgc                                                                                         <  5:217303/149‑1 (MQ=255)
                                                     gtgAGAGGACGGCGGGTGTGTATCCGCCTCCTACTCGATTGCGCGAAATCAGACCTGCCTTGTGTGCCCGCTATGTGAAGCAACCTTCGGTTGCGTTACATAGCGGACACACAGCGTCGTGACTTGCCGGGGAAACTACTTgg                                                                                             <  1:190853/143‑1 (MQ=255)
                                                     gtgAGAGGACGGCGGGTGTGTATCCGCCTCCTACTCGATTGCGCGAAATCAGACCTGCCTTGTGTGCCCGCTATGTGAAGCAACCTTCGGTTGCGTTACATAGCGGACACACAGCGTCGTGACTTGCCGGGGAAACTACTTgg                                                                                             >  2:190853/1‑143 (MQ=255)
                                                      tgAGAGGACGGCGGGTGTGAATCCGCCTCCTACTCGATTGCGCGAAATCAGTCCTGCCTTGTGTGCCCGCTATGTGAAGCAACCTTCGGTTGCGTTACATAGCGGACACACAGcgtcg                                                                                                                     <  7:66055/118‑1 (MQ=255)
                                                      tgAGAGGACGGCGGGTGTGAATCCGCCTCCTACTCGATTGCGCGAAATCAGTCCTGCCTTGTGTGCCCGCTATGTGAAGCAACCTTCGGTTGCGTTACATAGCGGACACACAGcgtcg                                                                                                                     >  8:66055/1‑118 (MQ=255)
                                                          agGACGGCGGGTGTGAATCCGCCTCCTACTCGATTGCGCGAAATCAGACCTGCCTTGTGTGCCCGCTATGTGAAGCAACCTTCGGTTGCGTTACATAGCGGACACACAGCGTCGTGACTTGCCGGGGAAACTACTTGGTCGCGGCgtgt                                                                                  <  5:8675/149‑1 (MQ=255)
                                                           ggACGGCGGGTGTGAATCCGCCTCCTACTCGATTGCGCGAAATCAGACCTGCCTTGTGTGCCCGCTATGTGAAGCAACCTTCGGTTGCGTTACATAGCGGACACACAGCGTCGTGACTTGCCGGGGAAACTACTTGGTCgcggc                                                                                      <  2:39823/144‑1 (MQ=255)
                                                           ggACGGCGGGTGTGAATCCGCCTCCTACTCGATTGCGCGAAATCAGACCTGCCTTGTGTGCCCGCTATGTGAAGCAACCTTCGGTTGCGTTACATAGCGGACACACAGCGTCGTGACTTGCCGGGGAAACTACTTGGTCgcggc                                                                                      >  1:39823/1‑144 (MQ=255)
                                                           ggACGGCGGGTGTGAATCCGCCTCCTACTCGATTGCGCGAAATCAGACCTGCCTTGTGTGCCCGCTATGTGAAGCAACCTTCGGTTGCGTTACATAGCGGACACACAGCGTCGTGACTTGCCGGGGAAACTACTTGGTCGCGGCGtgtg                                                                                 >  3:10275/1‑149 (MQ=255)
                                                             aCGGCGGGTGTGAATCCGCCTCCTACTCGATTGCGCGAAATCAGACCTGCCTTGTGTGCCCGCTATGTGAAGCAACCTTCGGTTGCGTTACATAGCGGACACACAGCGTCGTGACTTGCCGGGGAAACTACTTGGTCGCGGCGTGTGGa                                                                               <  4:233110/149‑1 (MQ=255)
                                                             aCGGCGGGTGTGAATCCGCCTCCTACTCGATTGCGCGAAATCAGACCTGCCTTGTGTGCCCGCTATGTGAAGCAACCTTCGGTTGCGTTACATAGCGGACACACAGCGTCGTGACTTGCCGGGGAAACTACTTGGTCGCGGCGTGTGGa                                                                               >  5:185480/1‑149 (MQ=255)
                                                                       tgAATCCGCCTCCTACTCGATTGCGCGAAATCAGACCTGCCTTGTGTGCCCGCTATGTGAAGCAACCTTCGGTTGCGTTACATAGCGGACACACAGCGTCGTGACTTGCCGGGGAAACTACTTGGTCGCGGCGTGTGGAGCAGAGACt                                                                      <  4:10275/148‑1 (MQ=255)
                                                                        gAATCCGCCTCCTACTCGATTGCGCGAAATCAGACCTGCCTTGTGTGCCCGCTATGTGAAGCAACCTTCGGTTGCGTTACATAGCGGACACACAGCGTCGTGACTTGCCGGGGAAACTACTTGGTCGCGGCGTGTGGAGCAGAGACTg                                                                     >  5:161901/1‑148 (MQ=255)
                                                                           tCCGCCTCCTACTCGATTGCGCGAAATCAGACCTGCCTTGTGTGCCCGGTATGTGAAGCAACCTTCGGTTGTGTTACATAGCGGACACACAGCGTCGTGACTTGCCGGGGAAACTACTTGGTCGCGGCGTGTGGAGCAGAGACTGTAgg                                                                 <  8:96316/149‑1 (MQ=255)
                                                                             cGCCTCCTACTCGATTGCGCGAAATCAGACCTGCCTTGTGTGCCCGCTATGTGAAGCAACCTTCGGTTGCGTTACATAGCGGACACACAGCGTCGTGACTTGCCGGGGAAACTACTTGGTCGCGGCGTGTGGAGCAGAGACTGTAggg                                                                <  8:84294/148‑1 (MQ=255)
                                                                                   ctACTCGATTGCGCGAAATCAGACCTGCCTTGTGTGCCCGCTATGTGAAGCAACCTTCGGTTGCGTTACATAGCGGACACACAGCGTCGTGACTTGCCGGGGAAACTACTTGGTCGCGGCGTGTGGAGCAGAGACTGTAGGGGAGCg                                                           <  7:221371/147‑1 (MQ=255)
                                                                                     aCTCGATTGCGCGAAATCAGACCTGCCTTGTGTGCCCGCTATGTGAAGCAACCTTCGGTTGCGTTACATAGCGGACACACAGCGTCGTGACTTGCCGGGGAAACTACTTGGTCGCGGCGTGTGGAGCAGAGACTGTAGGGGAGCg                                                           >  8:229502/1‑145 (MQ=255)
                                                                                     aCTCGATTGCGCGAAATCAGACCTGCCTTGTGTGCCCGCTATGTGAAGCAACCTTCGGTTGCGTTACATAGCGGACACACAGCGTCGTGACTTGCCGGGGAAACTACTTGGTCGCGGCGTGTGGAGCAGAGACTGTAGGGGAGCg                                                           <  7:229502/145‑1 (MQ=255)
                                                                                         gATTGCGCGAAATCAGACCTGCCTTGTGTGCCCGCTATGTGAAGCAACCTTCGGTTGCGTTACATAGCGGACACACAGCGTCGTGACTTGCCGGGGAAACTACTTGGTCGCGGCGTGTGGAGCAGAGACTGTAGGGGAGCGTTGACAgg                                                   >  2:190366/1‑148 (MQ=255)
                                                                                                 gAAATCAGACCTGCCTTGTGTGCCCGCTATGTGAAGCAACCTTCGGTTGCGTTACATAGCGGACACACAGCGTCGTGACTTGCCGGGGAAACTACTTGGTCGCGGCGTGTGGAGCAGAGACTGTAggg                                                                >  6:176877/1‑128 (MQ=255)
                                                                                                 gAAATCAGACCTGCCTTGTGTGCCCGCTATGTGAAGCAACCTTCGGTTGCGTTACATAGCGGACACACAGCGTCGTGACTTGCCGGGGAAACTACTTGGTCGCGGCGTGTGGAGCAGAGACTGTAggg                                                                <  5:176877/128‑1 (MQ=255)
                                                                                                             gCCTTGTGTGCCCGCTATGTGAAGCAACCTTCGGTTGCGTTAGATAGCGGACACACAGCGTCGTGACTTGCCGGGGCAACTACTTGGTCGCGGCGTGTGGAGCAGAGACTGTAGGGGAGCGTTGACAGTGGCGGt                                             >  3:162854/1‑135 (MQ=255)
                                                                                                             gCCTTCTGTGCCCGCTATGTGAAGCAACCTTCGGTTGCGTTACATAGGGGACACACAGCGTCGTGACTTGCCGGGGAAACTACTTGGTCGCGGCGTGTTGAGCAGAGACTGTAGGGGAGCGTTGACAGTGGCGGt                                             <  4:162854/135‑1 (MQ=255)
                                                                                                              ccTTGTGTGCCCGCTATGTGAAGCAACCTTCGGTTGCGTTACATAGCGGACACACAGCGTCGTGACTTGCCGGTGAAACTACTTGGTCGCGTCGTGTGGAGCAGAGACTGTAGGGGAGCGTTGACAGTGGCGGTTTCAAGCTAGCTTgg                              <  5:39284/149‑1 (MQ=255)
                                                                                                                     tgCCCGCTATGTGAAGCAACCTTCGGTTGCGTTACATAGCGGACACACAGCGTCGTGACTTGCCGGGGAAACTACTTGGTCGCGGCGTGTGGAGCAGAGACTGTAGGGGAGCg                                                           >  5:273500/1‑113 (MQ=255)
                                                                                                                     tgCCCGCTATGTGAAGCAACCTTCGGTTGCGTTACATAGCGGACACACAGCGTCGTGACTTGCCGGGGAAACTACTTGGTCGCGGCGTGTGGAGCAGAGACTGTAGGGGAGCg                                                           <  6:273500/113‑1 (MQ=255)
                                                                                                                         cGCTATGTGAAGCAACCTTCGGTTGCGTTACATAGCGGACACACAGCGTCGTGACTTGCCGGGGAAACTACTTGGTCGCGGCGTGTGGAGCAGAGACTGTAGGGGAGCGTTGACAGTGGCGGTTTCAAGCTAGCTTGGCGGTCACGCt                    >  1:264009/1‑148 (MQ=255)
                                                                                                                         cGCTATGTAACGCAACCGAAGGTTGCTTCACATAGCGGGCACACAGCGTCGTGACTTGCCGGGGAAACTACTTGGTCGCGGCGTGTGGAGCAGAGACTGTAGGGGAGCGTTGACAGTGGCGGTTTCAAGCTAGCTTGGCGGTCACGCt                    >  7:201726/1‑148 (MQ=37)
                                                                                                                          gCTATGTGAAGCAACCTTCGGTTGCGTTACATAGCGGACACACAGCGTCGTGACTTGCCGGGGAAACTACTTGGTCGCGGCGTGTGGAGCAGAGACTGTAGGGGAGCGTTGACAGTGGCGGTTTCAAGCTAGCTTGGCGGTCACGCTaa                  <  6:185480/149‑1 (MQ=255)
                                                                                                                          gCTATGTAACGCAACCGAAGGTTGCTTCACATAGCGGACACACAGCGTCGTGACTTGCCGGGGAAACTACTTGGTCGCGGCGTGTgg                                                                                <  6:83227/87‑1 (MQ=18)
                                                                                                                          gCTATGTAACGCAACCGAAGGTTGCTTCACATAGCGGACACACAGCGTCGTGACTTGCCGGGGAAACTACTTGGTCGCGGCGTGTgg                                                                                >  5:83227/1‑87 (MQ=18)
                                                                                                                              tgtgAAGCAACCTTCGGTTGCGTTACATAGCGGACACACAGCGTCGTGACTTGCCGGGGAAACTACTTGGTCGCGGCGTGTGGAGCAGAGACTGTAGGGGAGCGTTGACAGTGGCGGTTTCAAGCTAGCTTGGCGGTCACGCTAAGcc               >  4:171927/1‑148 (MQ=255)
                                                                                                                                tgAAGCAACCTTCGGTTGCGTTACATAGCGGACACACAGCGTCGTGACTTGCCGGGGAAACTACTTGGTCGCGGCGTGTGGAGCAGAGACTGTAGGGGAGCGTTGACAGTGGCGGTTTCAAGCTAGCTTGGCGGTCACGCTAAGCCGAg            <  6:161901/149‑1 (MQ=255)
                                                                                                                                   aGCAACCTTCGGTTGCGTTACATAGCGGACACACAGCGTCGTGACTTGCCGGGGAAACTACTTGGTCGCGGCGTGTGGAGCAGAGACTGTAGGGGAGCGTTGACAGTGGCGGTTTCAAGCTAGCTTGGCGGTCACGCTAAGCCGAGt           >  8:156069/1‑147 (MQ=255)
                                                                                                                                     cAACCTTCGGTTGCGTTACATAGCGGACACACAGCGTCGTGACTTGCCGGGGAAACTACTTGGTCGCGGCGTGTGGAGCAGAGACTGTAGGGGAGCGTTGACAGTGGCGGTTTCAAGCTAGCTTGGCGGTCACGCTAAGCCGAGTGAgg       <  3:171927/149‑1 (MQ=255)
                                                                                                                                            cGGTTGCGTTACATAGCGGACACACAGCGTCGTGACTTGCCGGGGAAACTACTTGGTCGCGGCGTGTGGAGCAGAGACTGTAGGGGAGCGTTGACAGTGGCGGTTTCAAGCTAGCTTGGCGGTCACGCTAAGCCGAGTGAGGCATTc  <  4:108466/147‑1 (MQ=255)
                                                                                                                                            cGGTTGCGTTACATAGCGGACACACAGCGTCCTGACTAGCCGGGGAAACTACTTGGTCGCGGCGTGTGGAGCAGAGACTGTAGGGGAGCGTTGACAGTGGCGGTTTCAAGCTAGCTTGGCGGTCACGCTAAGCCGAGTGAGGCATTc  <  7:94320/147‑1 (MQ=255)
                                                                                                                                                 gCGTTACATAGCGGACACACAGCGTCGTGACTTGCCGGGGAAACTACTTGGTCGCGGCGTGTGGAGCAGAGACTGTAgg                                                                 <  7:138627/79‑1 (MQ=255)
                                                                                                                                                 gCGTTACATAGCGGACACACAGCGTCGTGACTTGCCGGGGAAACTACTTGGTCGCGGCGTGTGGAGCAGAGACTGTAgg                                                                 >  8:138627/1‑79 (MQ=255)
                                                                                                                                                   |                                                                                                                                           
CGGCTTAGCCGCGTAACCTGAACCGCCGTGTACGGAACCGTACGCACGGTGGTGTGAGAGGACGGCGGGTGTGAATCCGCCTCCTACTCGATTGCGCGAAATCAGACCTGCCTTGTGTGCCCGCTATGTGAAGCAACCTTCGGTTGCGTTACATAGCGGACACACAGCGTCGTGACTTGCCGGGGAAACTACTTGGTCGCGGCGTGTGGAGCAGAGACTGTAGGGGAGCGTTGACAGTGGCGGTTTCAAGCTAGCTTGGCGGTCACGCTAAGCCGAGTGAGGCATTC  >  NC_002947/743301‑743587

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑