| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 743,450 | T→C | 7.7% | intergenic (+129/+145) | PP_0635 → / ← PP_0636 | group II intron‑encoding maturase/cold shock DNA‑binding domain‑containing protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 743,450 | 0 | T | C | 7.7% | 152.1 / 3.1 | 52 | intergenic (+129/+145) | PP_0635/PP_0636 | group II intron‑encoding maturase/cold shock DNA‑binding domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base T (23/25); new base C (2/2); total (25/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.54e-01 | |||||||||||
GGCTTAGCCGCGTAACCTGAACCGCCGTGTACGGAACCGTACGCACGGTGGTGTGAGAGGACGGCGGGTGTGAATCCGCCTCCTACTCGATTGCGCGAAATCAGACCTGCCTTGTGTGCCCGCTATGTGAAGCAACCTTCGGTTGCGTTACATAGCGGACACACAGCGTCGTGACTTGCCGGGGAAACTACTTGGTCGCGGCGTGTGGAGCAGAGACTGTAGGGGAGCGTTGACAGTGGCGGTTTCAAGCTAGCTTGGCGGTCACGCTAAGCCGAGTGAGGCATTCGATCCTGC > NC_002947/743302‑743595 | ggCTTAGCCGCGTAACCTGAACCGCCGTGTACGGAACCGTACGCACGGTGGTGTGAGAGGACGGCGGGTGTGAATCCGCCTCCTACTCGATTGCGCGAAATCAGACCTGCCTTGTGTGCCCGCTATGTGAAGCAACCTTCGGTTGCGtt < 3:223035/149‑1 (MQ=255) gCTTAGCCGCGTAACCTGAACCGCCGTGTACGGAACCGTACGCACGGTGGTGTGAGAGGACGGCGGGTGTGAATCCGCCTCCTACTCGATTGCGCGAAATCAGACCTGCCTTGTGTGCCCGCTATGTGAAGCAACCTTCGGTTGCGTTa < 2:152259/149‑1 (MQ=255) aCCTGAACCGCCGTGTACGGAACCGTACGCACGGTGGTGTGAGAGGACGGCGGGTGTGAATCCGCCTCCTACTCGATTGCGCGAAATCAGACCTGCCTTGTGTGCCCGCTATGTGAAGCAACCTTCGGTTGCGTTACATAGCGGacaca > 7:229505/1‑149 (MQ=255) cTGAACCGCCGTGTACGGAACCGTACGCACGGTGGTGTGAGAGGACGGCGGGTGTGAATCCGCCTCCTACTCGATTGCGCGAAATCAGACCTGCCTTGTGTGCCCGCTATGTGAAGCAACCTTCGGTTGCGTTACATAGCGGacaca > 6:8675/1‑147 (MQ=255) aaCCGCCGTGTACGGAACCGTACGCACGGTGGTGTGAGAGGACGGCGGGTGTGAATCCGCCTCCTACTCGATTGCGCGAAATCAGACCTGCCTTGTGTGTCCGCTATGTAACGCAACCGAAGGTTGCTTCACATAGCGGGcacacaagg < 1:79077/149‑4 (MQ=18) cgccgTGTACGGAACCGTACGCACGGTGGTGTGAGAGGACGGCGGGTGTGAATCCGCCTCCTACTCGATTGCGCGAAATCAGACCTGCCTTGTGTGCCCGCTATGTGAAGCAACCTTCGGTTGCGTTACATAGCGGACACACAGcgtcg < 8:229505/149‑1 (MQ=255) cgccgTGTACGGAACCGTACGCACGGGGGTGTGAGAGGACGGCGGGTGTGAATCCGCCTCGTACTCGATTGCGCGAATTCAGACCTGCCTTGTGTGCCCGCTATGTGAAGCAACCTTCGGGTGCGTTTCAGAGCGGGGACACAGcgggg > 8:94320/1‑146 (MQ=21) gccgTGTACGGAACCGTACGCACGGTGGTGTGAGAGGACGGCGGGTGTGAATCCGCCTCCTACTCGATTGCGCGAAATCAGACCTGCCTTGTGTGCCCGCTATGTGAAGCAACCTTCGGTTGCGTTACATAGCGGACACACAGcgtcgt < 3:191146/149‑1 (MQ=255) ccgTGTACGGAACCGTACGCACGGTGGTGTGAGAGGACGGCGGGTGTGAATCCGCCTCCTACTCGATTGCGCGAAATCAGACCTGCCTTGTGTGCCCGCTATGTGAAGCAACCTTCGGTTGCGTTACATAGCGGACACACAGcgtcgt > 4:34938/1‑148 (MQ=255) ggAACCGTACGCACGGTGGTGTGAGAGGACGGCGGGTGTGAATCCGCCTCCTACTCGATTGCGCGAAATCAGACCTGCCTTGTGTGCCCGCTATGTGAAGCAACCTTCGGTTGCGTTACATAGCGGACACACAGCGTCGTGACTTGCCg < 3:34938/149‑1 (MQ=255) ccGTACGCACGGTGGTGTGAGAGGACGGCGGGTGTGAATCCGCCTCCTACTCGATTGCGCGAAATCAGACCTGCCTTGTGTGCCCGCTATGTGAAGCAACCTTCGGTTGCGTTACATAGCGGACACACAGCGTCGTGACTTGCCgggg > 6:251011/1‑148 (MQ=255) ccGTACGCACGGTGGTGTGAGAGGACGGCGGGTGTGAATCCGCCTCCTACTCGATTGCGCGAAATCAGACCTGCCTTGTGTGACCGCAATGTGAATCAACCATCGGTTGCGTTACAAAGACGACACACAACGAAGTTACATTCCgggg > 6:39284/1‑148 (MQ=21) gCACGGTGGTGTGAGAGGACGGCGGGTGTGAATCCGCCTCCTACTCGATTGCGCGAAATCAGACCTGCCTTGTGTGCCCGCTATGTGAAGCAACCTTCGGTTGCGTTACATAGCGGACACACAGCGTCGTGACTTGCCGGGGAAactac > 6:217303/1‑149 (MQ=255) aCGGTGGTGTGAGAGGACGGCGGGTGTGAATCCGCCTCCTACTCGATTGCGCGAAATCAGACCTGCCTTGTGTGCCCGCTATGTGAAGCAACCTTCGGTTGCGTTACATAGCGGACACACAGCGTCGTGACTTGCCGGGGAAACTACtt > 3:108466/1‑149 (MQ=255) gtgtGAGAGGACGGCGGGTGTGAATCCGCCTCCTACTCGATTGCGCGAAATCAGACCTGCCTTGTGTGCCCGCTATGTGAAGCAACCTTCGGTTGCGTTACATAGCGGACACACAGCGTCGTGACTTGCCGGGGAAACTACTTGGTcgc < 5:217303/149‑1 (MQ=255) gtgAGAGGACGGCGGGTGTGTATCCGCCTCCTACTCGATTGCGCGAAATCAGACCTGCCTTGTGTGCCCGCTATGTGAAGCAACCTTCGGTTGCGTTACATAGCGGACACACAGCGTCGTGACTTGCCGGGGAAACTACTTgg < 1:190853/143‑1 (MQ=255) gtgAGAGGACGGCGGGTGTGTATCCGCCTCCTACTCGATTGCGCGAAATCAGACCTGCCTTGTGTGCCCGCTATGTGAAGCAACCTTCGGTTGCGTTACATAGCGGACACACAGCGTCGTGACTTGCCGGGGAAACTACTTgg > 2:190853/1‑143 (MQ=255) tgAGAGGACGGCGGGTGTGAATCCGCCTCCTACTCGATTGCGCGAAATCAGTCCTGCCTTGTGTGCCCGCTATGTGAAGCAACCTTCGGTTGCGTTACATAGCGGACACACAGcgtcg < 7:66055/118‑1 (MQ=255) tgAGAGGACGGCGGGTGTGAATCCGCCTCCTACTCGATTGCGCGAAATCAGTCCTGCCTTGTGTGCCCGCTATGTGAAGCAACCTTCGGTTGCGTTACATAGCGGACACACAGcgtcg > 8:66055/1‑118 (MQ=255) agGACGGCGGGTGTGAATCCGCCTCCTACTCGATTGCGCGAAATCAGACCTGCCTTGTGTGCCCGCTATGTGAAGCAACCTTCGGTTGCGTTACATAGCGGACACACAGCGTCGTGACTTGCCGGGGAAACTACTTGGTCGCGGCgtgt < 5:8675/149‑1 (MQ=255) ggACGGCGGGTGTGAATCCGCCTCCTACTCGATTGCGCGAAATCAGACCTGCCTTGTGTGCCCGCTATGTGAAGCAACCTTCGGTTGCGTTACATAGCGGACACACAGCGTCGTGACTTGCCGGGGAAACTACTTGGTCgcggc < 2:39823/144‑1 (MQ=255) ggACGGCGGGTGTGAATCCGCCTCCTACTCGATTGCGCGAAATCAGACCTGCCTTGTGTGCCCGCTATGTGAAGCAACCTTCGGTTGCGTTACATAGCGGACACACAGCGTCGTGACTTGCCGGGGAAACTACTTGGTCgcggc > 1:39823/1‑144 (MQ=255) ggACGGCGGGTGTGAATCCGCCTCCTACTCGATTGCGCGAAATCAGACCTGCCTTGTGTGCCCGCTATGTGAAGCAACCTTCGGTTGCGTTACATAGCGGACACACAGCGTCGTGACTTGCCGGGGAAACTACTTGGTCGCGGCGtgtg > 3:10275/1‑149 (MQ=255) aCGGCGGGTGTGAATCCGCCTCCTACTCGATTGCGCGAAATCAGACCTGCCTTGTGTGCCCGCTATGTGAAGCAACCTTCGGTTGCGTTACATAGCGGACACACAGCGTCGTGACTTGCCGGGGAAACTACTTGGTCGCGGCGTGTGGa < 4:233110/149‑1 (MQ=255) aCGGCGGGTGTGAATCCGCCTCCTACTCGATTGCGCGAAATCAGACCTGCCTTGTGTGCCCGCTATGTGAAGCAACCTTCGGTTGCGTTACATAGCGGACACACAGCGTCGTGACTTGCCGGGGAAACTACTTGGTCGCGGCGTGTGGa > 5:185480/1‑149 (MQ=255) tgAATCCGCCTCCTACTCGATTGCGCGAAATCAGACCTGCCTTGTGTGCCCGCTATGTGAAGCAACCTTCGGTTGCGTTACATAGCGGACACACAGCGTCGTGACTTGCCGGGGAAACTACTTGGTCGCGGCGTGTGGAGCAGAGACt < 4:10275/148‑1 (MQ=255) gAATCCGCCTCCTACTCGATTGCGCGAAATCAGACCTGCCTTGTGTGCCCGCTATGTGAAGCAACCTTCGGTTGCGTTACATAGCGGACACACAGCGTCGTGACTTGCCGGGGAAACTACTTGGTCGCGGCGTGTGGAGCAGAGACTg > 5:161901/1‑148 (MQ=255) tCCGCCTCCTACTCGATTGCGCGAAATCAGACCTGCCTTGTGTGCCCGGTATGTGAAGCAACCTTCGGTTGTGTTACATAGCGGACACACAGCGTCGTGACTTGCCGGGGAAACTACTTGGTCGCGGCGTGTGGAGCAGAGACTGTAgg < 8:96316/149‑1 (MQ=255) cGCCTCCTACTCGATTGCGCGAAATCAGACCTGCCTTGTGTGCCCGCTATGTGAAGCAACCTTCGGTTGCGTTACATAGCGGACACACAGCGTCGTGACTTGCCGGGGAAACTACTTGGTCGCGGCGTGTGGAGCAGAGACTGTAggg < 8:84294/148‑1 (MQ=255) ctACTCGATTGCGCGAAATCAGACCTGCCTTGTGTGCCCGCTATGTGAAGCAACCTTCGGTTGCGTTACATAGCGGACACACAGCGTCGTGACTTGCCGGGGAAACTACTTGGTCGCGGCGTGTGGAGCAGAGACTGTAGGGGAGCg < 7:221371/147‑1 (MQ=255) aCTCGATTGCGCGAAATCAGACCTGCCTTGTGTGCCCGCTATGTGAAGCAACCTTCGGTTGCGTTACATAGCGGACACACAGCGTCGTGACTTGCCGGGGAAACTACTTGGTCGCGGCGTGTGGAGCAGAGACTGTAGGGGAGCg > 8:229502/1‑145 (MQ=255) aCTCGATTGCGCGAAATCAGACCTGCCTTGTGTGCCCGCTATGTGAAGCAACCTTCGGTTGCGTTACATAGCGGACACACAGCGTCGTGACTTGCCGGGGAAACTACTTGGTCGCGGCGTGTGGAGCAGAGACTGTAGGGGAGCg < 7:229502/145‑1 (MQ=255) gATTGCGCGAAATCAGACCTGCCTTGTGTGCCCGCTATGTGAAGCAACCTTCGGTTGCGTTACATAGCGGACACACAGCGTCGTGACTTGCCGGGGAAACTACTTGGTCGCGGCGTGTGGAGCAGAGACTGTAGGGGAGCGTTGACAgg > 2:190366/1‑148 (MQ=255) gAAATCAGACCTGCCTTGTGTGCCCGCTATGTGAAGCAACCTTCGGTTGCGTTACATAGCGGACACACAGCGTCGTGACTTGCCGGGGAAACTACTTGGTCGCGGCGTGTGGAGCAGAGACTGTAggg < 5:176877/128‑1 (MQ=255) gAAATCAGACCTGCCTTGTGTGCCCGCTATGTGAAGCAACCTTCGGTTGCGTTACATAGCGGACACACAGCGTCGTGACTTGCCGGGGAAACTACTTGGTCGCGGCGTGTGGAGCAGAGACTGTAggg > 6:176877/1‑128 (MQ=255) gCCTTGTGTGCCCGCTATGTGAAGCAACCTTCGGTTGCGTTAGATAGCGGACACACAGCGTCGTGACTTGCCGGGGCAACTACTTGGTCGCGGCGTGTGGAGCAGAGACTGTAGGGGAGCGTTGACAGTGGCGGt > 3:162854/1‑135 (MQ=255) gCCTTCTGTGCCCGCTATGTGAAGCAACCTTCGGTTGCGTTACATAGGGGACACACAGCGTCGTGACTTGCCGGGGAAACTACTTGGTCGCGGCGTGTTGAGCAGAGACTGTAGGGGAGCGTTGACAGTGGCGGt < 4:162854/135‑1 (MQ=255) ccTTGTGTGCCCGCTATGTGAAGCAACCTTCGGTTGCGTTACATAGCGGACACACAGCGTCGTGACTTGCCGGTGAAACTACTTGGTCGCGTCGTGTGGAGCAGAGACTGTAGGGGAGCGTTGACAGTGGCGGTTTCAAGCTAGCTTgg < 5:39284/149‑1 (MQ=255) tgCCCGCTATGTGAAGCAACCTTCGGTTGCGTTACATAGCGGACACACAGCGTCGTGACTTGCCGGGGAAACTACTTGGTCGCGGCGTGTGGAGCAGAGACTGTAGGGGAGCg < 6:273500/113‑1 (MQ=255) tgCCCGCTATGTGAAGCAACCTTCGGTTGCGTTACATAGCGGACACACAGCGTCGTGACTTGCCGGGGAAACTACTTGGTCGCGGCGTGTGGAGCAGAGACTGTAGGGGAGCg > 5:273500/1‑113 (MQ=255) cGCTATGTGAAGCAACCTTCGGTTGCGTTACATAGCGGACACACAGCGTCGTGACTTGCCGGGGAAACTACTTGGTCGCGGCGTGTGGAGCAGAGACTGTAGGGGAGCGTTGACAGTGGCGGTTTCAAGCTAGCTTGGCGGTCACGCt > 1:264009/1‑148 (MQ=255) cGCTATGTAACGCAACCGAAGGTTGCTTCACATAGCGGGCACACAGCGTCGTGACTTGCCGGGGAAACTACTTGGTCGCGGCGTGTGGAGCAGAGACTGTAGGGGAGCGTTGACAGTGGCGGTTTCAAGCTAGCTTGGCGGTCACGCt > 7:201726/1‑148 (MQ=37) gCTATGTGAAGCAACCTTCGGTTGCGTTACATAGCGGACACACAGCGTCGTGACTTGCCGGGGAAACTACTTGGTCGCGGCGTGTGGAGCAGAGACTGTAGGGGAGCGTTGACAGTGGCGGTTTCAAGCTAGCTTGGCGGTCACGCTaa < 6:185480/149‑1 (MQ=255) gCTATGTAACGCAACCGAAGGTTGCTTCACATAGCGGACACACAGCGTCGTGACTTGCCGGGGAAACTACTTGGTCGCGGCGTGTgg < 6:83227/87‑1 (MQ=18) gCTATGTAACGCAACCGAAGGTTGCTTCACATAGCGGACACACAGCGTCGTGACTTGCCGGGGAAACTACTTGGTCGCGGCGTGTgg > 5:83227/1‑87 (MQ=18) tgtgAAGCAACCTTCGGTTGCGTTACATAGCGGACACACAGCGTCGTGACTTGCCGGGGAAACTACTTGGTCGCGGCGTGTGGAGCAGAGACTGTAGGGGAGCGTTGACAGTGGCGGTTTCAAGCTAGCTTGGCGGTCACGCTAAGcc > 4:171927/1‑148 (MQ=255) tgAAGCAACCTTCGGTTGCGTTACATAGCGGACACACAGCGTCGTGACTTGCCGGGGAAACTACTTGGTCGCGGCGTGTGGAGCAGAGACTGTAGGGGAGCGTTGACAGTGGCGGTTTCAAGCTAGCTTGGCGGTCACGCTAAGCCGAg < 6:161901/149‑1 (MQ=255) aGCAACCTTCGGTTGCGTTACATAGCGGACACACAGCGTCGTGACTTGCCGGGGAAACTACTTGGTCGCGGCGTGTGGAGCAGAGACTGTAGGGGAGCGTTGACAGTGGCGGTTTCAAGCTAGCTTGGCGGTCACGCTAAGCCGAGt > 8:156069/1‑147 (MQ=255) cAACCTTCGGTTGCGTTACATAGCGGACACACAGCGTCGTGACTTGCCGGGGAAACTACTTGGTCGCGGCGTGTGGAGCAGAGACTGTAGGGGAGCGTTGACAGTGGCGGTTTCAAGCTAGCTTGGCGGTCACGCTAAGCCGAGTGAgg < 3:171927/149‑1 (MQ=255) cGGTTGCGTTACATAGCGGACACACAGCGTCGTGACTTGCCGGGGAAACTACTTGGTCGCGGCGTGTGGAGCAGAGACTGTAGGGGAGCGTTGACAGTGGCGGTTTCAAGCTAGCTTGGCGGTCACGCTAAGCCGAGTGAGGCATTc < 4:108466/147‑1 (MQ=255) cGGTTGCGTTACATAGCGGACACACAGCGTCCTGACTAGCCGGGGAAACTACTTGGTCGCGGCGTGTGGAGCAGAGACTGTAGGGGAGCGTTGACAGTGGCGGTTTCAAGCTAGCTTGGCGGTCACGCTAAGCCGAGTGAGGCATTc < 7:94320/147‑1 (MQ=255) gCGTTACATAGCGGACACACAGCGTCGTGACTTGCCGGGGAAACTACTTGGTCGCGGCGTGTGGAGCAGAGACTGTAgg < 7:138627/79‑1 (MQ=255) gCGTTACATAGCGGACACACAGCGTCGTGACTTGCCGGGGAAACTACTTGGTCGCGGCGTGTGGAGCAGAGACTGTAgg > 8:138627/1‑79 (MQ=255) ttACATAGCGGACACACAGCGTCGTGACTTGCCGGGGAAACTACTTGGTCGCGGCGTGTGGAGCAGAGACTGTAGGGGAGCGTTGACAGTGGCGGTTTCAAGCTAGCTTGGCGGTCACGCTAAGCCGAGTGAGGCATTCGATCCTGc > 3:239923/1‑147 (MQ=255) | GGCTTAGCCGCGTAACCTGAACCGCCGTGTACGGAACCGTACGCACGGTGGTGTGAGAGGACGGCGGGTGTGAATCCGCCTCCTACTCGATTGCGCGAAATCAGACCTGCCTTGTGTGCCCGCTATGTGAAGCAACCTTCGGTTGCGTTACATAGCGGACACACAGCGTCGTGACTTGCCGGGGAAACTACTTGGTCGCGGCGTGTGGAGCAGAGACTGTAGGGGAGCGTTGACAGTGGCGGTTTCAAGCTAGCTTGGCGGTCACGCTAAGCCGAGTGAGGCATTCGATCCTGC > NC_002947/743302‑743595 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |