Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 780,560 G→T 8.5% intergenic (‑90/+32) PP_0670 ← / ← glyA‑II bile acid/Na+ symporter family transporter/serine hydroxymethyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947780,5600GT 8.5% 141.4 / 4.4 47intergenic (‑90/+32)PP_0670/glyA‑IIbile acid/Na+ symporter family transporter/serine hydroxymethyltransferase
Reads supporting (aligned to +/- strand):  ref base G (22/21);  new base T (2/2);  total (24/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.12e-01

CCAGTACGGCGGCGAAGTAGATGGGGATCTGCCGGGTTTCGAGTTGCTCTCTGGTCAAGGTGCGGCCTGTGCAAAGTGGAGGTATTGGGGGCGCAGTCTTTGTACGAGGGGCGGTGCGCCTGTATTAAACGAAAGAGCCCGGCACTGGGCCGGGCTCTTTCACTTTAGGCAAGCGCCAATCAGTTGCCGTAAACCGGCAGCTTCTTGCAGATGGCCTTGACCTTCTCACGGACGGCGTCGATCACCGCTTCGTTGTTCAGGTCGGCCAGGATGTCGCAGATCCAGCCGG  >  NC_002947/780413‑780701
                                                                                                                                                   |                                                                                                                                             
acAGTACGGCGGCGAAGTAGATGGGGATCTGCCGGGTTTCGAGTTGCTCTCTTGTCAAGGTGCGGCCTGTGCAAAGTGGAGGTATTGGGGGCGCAGTCTTTGTACGAGGGGCGGTGCGCCTGTATTAAACGAAAGAGCCCGGCACTggg                                                                                                                                              <  6:2657/148‑1 (MQ=255)
 cAGTACGGCGGCGAAGTAGATGGGGATCTGCCGGGTTTCGAGTTGCTCTCTGGTCAAGGTGCGGCCTGTGCAAAGTGGAGGTATTGGGGGCGCAGTCTTTGTACGAGGGGCGGTGCGCCTGTATTAAACGAAAGAGCCCGGCACTGGGc                                                                                                                                             <  7:109269/149‑1 (MQ=255)
      cggcggCGAAGTAGATGGGGAGCTGCCGGGTTTCGAGGTGCTCTCTGGTCACGGGGCGGCCTGTGCAAAGTGGAGGTATTGGGGGCGCAGGCTTTGTACGAGGGGCGGTGCGCCTGTATTAAACGAAAGAGCCCGGCACTGGGCCggg                                                                                                                                         <  3:193401/148‑1 (MQ=255)
      cgccggcgAAGTAGATGCGGATCTGCCGGGTATCGAGTTGCTCTCTGGTCAAGCTGCGGCCTGTGCAAAGTGGAGGTATTGGGGGCGCAGTCTTTGTACGAGGGGCGGTGCGCCTGTATTAAACGAAAGAGCCCGGCACTGGGCCggg                                                                                                                                         <  6:254975/145‑1 (MQ=255)
           gcgAAGTAGATGGGGATCTGCCGGGTTTCGAGTTGCTCTCTGGTCAAGGTGCGGCCTGTGCAAAGTGGAGGTATTGGGGGCGCAGTCTTTGTACGAGGGGCGGTGCGCCTGTATTAAACGAAAGAGCCCGGCCCAGTGCCGGGCTCttt                                                                                                                                   <  4:185189/149‑1 (MQ=255)
           gcgAAGTAGATGGGGATCTGCCGGGTTTCGAGTTGCTCTCTGGTCAAGGTGCGGCCTGTGCAAAGTGGAGGTATTGGGGGCGCAGTCTTTGTACGAGGGGCGGTGCGCCTGTATTAAACGAAAGAGCCCGGCACTGGGCCGGGCTCttt                                                                                                                                   <  5:149030/149‑1 (MQ=255)
              aaGTAGATGGGGATCTGCCGGGTTTCGAGTTGCTCTCTGGTCAAGGTGCGGCCTGTGCAAAGTGGAGGTATTGGGGGCGCAGTCTTTGTACGAGGGGCGGTGCGCCTGTATTAAACGAAAGAGCCCGGCCCAGTGCCGGGCTCTTTcgt                                                                                                                                <  2:203979/149‑3 (MQ=255)
               aGTAGATGGGGATCTGCCGGGTTTCGAGTTGCTCTCTGGTCAAGGTGCGGCCTGTGCAAAGTGGAGGTATTGGGGGCGCAGTCTTTGTACGAGGGGCGGTGCGCCTGTATTAAACGAAAGAGCCCGGCACTGGGCCGGGCTCTTTCACt                                                                                                                               >  4:35590/1‑149 (MQ=255)
                   gATGGGGATCTGCCGGGTTTCGAGTTGCTCTCTGGTCAAGGTGCGGCCTGTGCAAAGTGGAGGTATTGGGGGCGCAGTCTTTGTACGAGGGGCGGTGCGCCTGTATTAAACGAAAGAGCCCGGCACTGGGCCGGGCTCTTTCACTTTa                                                                                                                            <  8:88691/148‑1 (MQ=255)
                    aTGGGGATCTGCCGGGTTTCGAGTTGCTCTCTGGTCAAGGTGCGGCCTGTGCAAAGTGGAGGTATTGGGGGCGCAGTCTTTGTACGAGGGGCGGTGCGCCTGTATTAAACGAAAGAGCCCGGCACTGGGCCGGGCTCTTTCACTTTAgg                                                                                                                          >  1:183013/1‑149 (MQ=255)
                             tGCCGGGTTTCGAGTTGCTCTCTGGTCAAGGTGCGGCCTGTGCAAAGTGGAGGTATTGGGGGCGCAGTCTTTGTACGAGGGGCGGTGCGCCTGTATTAAACGAAAGAGCCCGGCACTGGGCCGGGctct                                                                                                                                     <  7:21921/129‑1 (MQ=255)
                             tGCCGGGTTTCGAGTTGCTCTCTGGTCAAGGTGCGGCCTGTGCAAAGTGGAGGTATTGGGGGCGCAGTCTTTGTACGAGGGGCGGTGCGCCTGTATTAAACGAAAGAGCCCGGCACTGGGCCGGGctct                                                                                                                                     >  8:21921/1‑129 (MQ=255)
                               ccGGGTTTCGAGTTGCTCTCTGGTCAAGGTGCGGCCTGTGCAAAGTGGAGGTATTGGGGGCGCAGTCTTTGTACGAGGGGCGGTGCGCCTGTATTAAACGAAAGAGCCCGGCACTGGGCCGGGCTCTTTCACTTTAGGCAAGCGCCAAt                                                                                                               >  6:19677/1‑149 (MQ=255)
                                cGGGTTTCGAGTTGCTCTCTGGTCAAGGTGCGGCCTGTGCAAAGTGGAGGTATTGGGGGCGCAGTCTTTGTACGAGGGGCGGTGCGCCTGTATTAAACGAAAGAGCCCGGCACTGGGCCGGGCTCTTTCACTTTAGGCAAGCGCCAAt                                                                                                               >  5:68264/1‑148 (MQ=255)
                                     ttCGAGTTGCTCTCTGGTCAAGGTGCGGCCTGTGCAAAGTGGAGGTATTGGGGGCGCAGTCTTTGTACGAGGGGCGGTGCGCCTGTATTAAACGAAAGAGCCCGGCACTGGGCCGGGCTCTTTCACTTTAGGCAAGCGCCAATCAGTTg                                                                                                         >  6:198614/1‑149 (MQ=255)
                                       cGAGTTGCTCTCTGGTCAAGGTGCGGCCTGTGCAAAGTGGAGGTATTGGGGGCGCAGTCTTTGTACGAGGGGCGGTGCGCCTGTATTAAACGAAAGAGCCCGGCACTGGGCCGGGCTCTTTCACTTTAGGCAAGCGCCAATCAGTTGcc                                                                                                       >  1:2340/1‑149 (MQ=255)
                                              ctctctGGTCAAGGTGCGGCCTGTGCAAAGTGGAGGTATTGGGGGCGCAGTCTTTGTACGAGGGGCGGTGCGCCTGTATTAAACGAAAGAGCCCGGCACTGGGCCGGGCTCTTTCACTTTAGGCAAGCGCCAATCAGTTGCCGTAAAcc                                                                                                >  1:90700/1‑149 (MQ=255)
                                                     gTCAAGGTGCGGCCTGTGCAAAGTGGAGGTATTGGGGGCGCAGTCTTTGTACGAGGGGCGGTGCGCCTGTATTAAACGAAAGAGCCCGGCACTGGGCCGGGCTCTTTCACTTTAGGCAAGCGCCAATCAGTTGCCGTAAACCGGCAGct                                                                                         >  3:31271/1‑149 (MQ=255)
                                                      tCAAGGTGCGGCCTGTGCAAAGTGGAGGTATTTGTGGCGAAGTCTTTGTACGAGGGGCGGTGCGCCTGTATTAAAAGAAAGAGCCCGGCACTGGGCCGGGCTCTTTCACTTTAGGCAAGAGCCAATCAGTTGCCGTAAAACGGCAGct                                                                                         >  8:175632/1‑148 (MQ=255)
                                                        aaGGTGCGGCCTGTGCAAAGTGGAGGTATTGGGGGCGCAGTCTTTGTACGAGGGGCGGTGCGCCTGTATTAAACGAAAGAGCCCGGCACTGGGCCGGGCTCTTTCACTTTAGGCAAGCGCCAATCAGTTGCCGTAAACCGGCAGcttc                                                                                       <  3:35590/148‑1 (MQ=255)
                                                          ggTGCGGCCTGTGCAAAGTGGAGGTATTGGGGGCGCAGTCTTTGTACGAGGGGCGGTGCGCCTGTATTAAACGAAAGAGCCCGGCACTGGGCCGGGCTCTTTCACTTTAGGCAAGCGCCAATCAGTTGCCGTAAACCGGCAGCTTCTTg                                                                                    <  5:19677/149‑1 (MQ=255)
                                                              cGGCCTGTGCAAAGTGGAGGTATTGGGGGCGCAGTCTTTGTACGAGGGGCGGTGCGCCTGTATTAAACGAAAGAGCCCGGCACTGGGCCGGGCTCTTTCACTTTAGGCAAGCGCCAATCAGTTGCCGTAAACCGGCAGCTTCTTGCAGa                                                                                <  4:31271/149‑1 (MQ=255)
                                                                gCCTGTGCAAAGTGGAGGTATTGGGGGCGCAGTCTTTGTACGAGGGGCGGTGCGCCTGTATTAAACGAAAGAGCCCGGCACTGGGCCGGGCTCttt                                                                                                                                   >  2:119392/1‑96 (MQ=255)
                                                                gCCTGTGCAAAGTGGAGGTATTGGGGGCGCAGTCTTTGTACGAGGGGCGGTGCGCCTGTATTAAACGAAAGAGCCCGGCACTGGGCCGGGCTCttt                                                                                                                                   <  1:119392/96‑1 (MQ=255)
                                                                   tgtgCAAAGTGGAGGTATTGGGGGCGCAGTCTTTGTACGAGGGGCGGTGCGCCTGTATTAAACGAAAGAGCCCGGCACTGGGCCGGGCTCTTTCACTTTAGGCAAGCGCCAATCAGTTGCCGTAAACCGGCAGCTTCTTGCAGATGGcc                                                                           <  2:2340/149‑1 (MQ=255)
                                                                       cAAAGTGGAGGTATTGGGGGCGCAGTCTTTGTACGAGGGGCGGTGCGCCTGTATTAAACGAAAGAGCCCGGCACTGGGCCGGGCTCTTTCACTTTAGGCAAGCGCCAATCAGTTGCCGTAAACCGGCAGCTTCTTGCAGATGGCCTTg                                                                        <  6:68264/148‑1 (MQ=255)
                                                                       cAAAGTGGAGGTATTGGGGGCGCAGTCTTTGTACGAGGGGCGGTGCGCCTGTATTAAACGAAAGAGCCCGGCACTGGGCCGGGCTCTTTCACTTTAGGCAAGCGCCAATCAGTTGCCGTAAACCGGCAGCTTCTTGCAGATGGCCTTGa                                                                       >  2:108834/1‑149 (MQ=255)
                                                                        aaaGTGGAGGTATTGGGGGCGCAGTCTTTGTACGAGGGGCGGTGCGCCTGTATTAAACGAAAGAGCCCGGCACTGGGCCGGGCTCTTTCACTTTAGGCAAGCGCCAATCa                                                                                                             <  8:92501/110‑1 (MQ=255)
                                                                        aaaGTGGAGGTATTGGGGGCGCAGTCTTTGTACGAGGGGCGGTGCGCCTGTATTAAACGAAAGAGCCCGGCACTGGGCCGGGCTCTTTCACTTTAGGCAAGCGCCAATCa                                                                                                             >  7:92501/1‑110 (MQ=255)
                                                                                 gTATTGGGGGCGCAGTCTTTGTACGAGGGGCGGTGCGCCTGTATTAAACGAAAGAGCCCGGCACTGGGCCGGGCTCTTTCACTTTAGGCAAGCGCCAATCAGTTGCCGTAAACCGGCAGCTTCTTGCAGATGGCCtt                                                                         >  1:268849/1‑137 (MQ=255)
                                                                                 gTATTGGGGGCGCAGTCTTTGTACGAGGGGCGGTGCGCCTGTATTAAACGAAAGAGCCCGGCACTGGGCCGGGCTCTTTCACTTTAGGCAAGCGCCAATCAGTTGCCGTAAACCGGCAGCTTCTTGCAGATGGCCtt                                                                         <  2:268849/137‑1 (MQ=255)
                                                                                       ggggCGCAGTCTTTGTACGAGGGGCGGTGCGCCTGTATTAAACGAAAGAGCCCGGCACTGGGCCGGGCTCTTTCACTTTAGGCAAGCGCCAATCAGTTGCCg                                                                                                      <  1:193744/102‑1 (MQ=255)
                                                                                       ggggCGCAGTCTTTGTACGAGGGGCGGTGCGCCTGTATTAAACGAAAGAGCCCGGCACTGGGCCGGGCTCTTTCACTTTAGGCAAGCGCCAATCAGTTGCCg                                                                                                      >  2:193744/1‑102 (MQ=255)
                                                                                            gcAGTCTTTGTACGAGGGGCGGTGCGCCTGTATTAAACGAAAGAGCCCGGCACTGGGCCGGGCTCTTTCACTTTAGGCAAGCGCCAATCAGTTGCCGTAAACCGGCAGCTTCTTGCAGATGGCCTTGACCTTCTCACGGACGGCGTCGa                                                  >  7:2532/1‑149 (MQ=255)
                                                                                              aGTCTTTGTACGAGGGGCGGTGCGCCTGTATTAAACGAAAGAGCCCGGCACTGGGCCGGGCTCTTTCACTTTAGGCAAGCGCCAATCAGTTGCCGTAAACCGGCAGCTTCTTGCAGATGGCCTTGACCTTCTCACGGACGGCGTc                                                    <  3:105701/145‑1 (MQ=255)
                                                                                              aGTCTTTGTACGAGGGGCGGTGCGCCTGTATTAAACGAAAGAGCCCGGCACTGGGCCGGGCTCTTTCACTTTAGGCAAGCGCCAATCAGTTGCCGTAAACCGGCAGCTTCTTGCAGATGGCCTTGACCTTCTCACGGACGGCGTc                                                    >  4:105701/1‑145 (MQ=255)
                                                                                                   ttGTACGAGGGCCGGTGCGCCTGTATTAAACGAAAGAGCCCGGCACTCGCCCGGGCACATTCACATTAGGCAAGCCCCAAACAGTTGCCGTAAACCGGCACCATCTTGCAGATGGCCTTGACCTTCTCACGGACGGCGACGATCACCg                                            >  6:38595/1‑148 (MQ=255)
                                                                                                         gAGGGGCGGTGCGCCTGTATTAAACGAAAGAGCCCGGCACTGGGCCGGGCTCTTTCACTTTAGGCAAGCGCCAATCAg                                                                                                            >  5:42016/1‑78 (MQ=255)
                                                                                                         gAGGGGCGGTGCGCCTGTATTAAACGAAAGAGCCCGGCACTGGGCCGGGCTCTTTCACTTTAGGCAAGCGCCAATCAg                                                                                                            <  6:42016/78‑1 (MQ=255)
                                                                                                                ggTGCGCCTGTATTAAACGAAAGAGCCCGGCACTGGGCCGGGCTCTTTCACTTTAGGCAAGCGCCAATCAGTTGCCGTAAACCGGCAGCTTCTTGCAGATGGCCTTGACCTTCTCACGGACGGCGt                                                     <  8:25534/126‑1 (MQ=255)
                                                                                                                            ttAAACGAAAGAGCCCGGCACTGGGCCGGGCTCTTTCACTTTAGGCAAGCGCCAATCAGTTGCCGTAAACCGGCAGCTTCTTGCAGATGGCCTTGACCTTCTCACGGACGGCGTCGATc                                                >  5:143755/1‑119 (MQ=255)
                                                                                                                            ttAAACGAAAGAGCCCGGCACTGGGCCGGGCTCTTTCACTTTAGGCAAGCGCCAATCAGTTGCCGTAAACCGGCAGCTTCTTGCAGATGGCCTTGACCTTCTCACGGACGGCGTCGATc                                                <  6:143755/119‑1 (MQ=255)
                                                                                                                             tAAACGAAAGAGCCCGGCACTGGGCCGGGCTCTTTCACTTTAGGCAAGCGCCAATCAGTTGCCGTAAACCGGCAGCTTCTTGCAGATGGCCTTGACCTTCTCACGTACGGCGTCGATCACCGCTTCGTTGTTCAGGTCGGCCAGGATg                  <  2:90700/148‑1 (MQ=255)
                                                                                                                                  gAAAGAGCCCGGCCCAGTGCCGGGCTCTTTCACTTTAGGCAAGCGCCAATCAGTTGCCGTAAACCGGCAGCTTCTTGCAGATGGCCTTGACCTTCTCACGGACGGCGTCGATCACCGCTTCGTTGTTCAGGTCGGCCAGGATGTCg               >  7:86576/1‑146 (MQ=255)
                                                                                                                                   aaaGAGCCCGGCCCAGTGCCGGGCTCTTTCACTTTAGGCAAGCGCCAATCAGTTGCCGTAAACCGGCAGCTTCTTGCAGATGGCCTTGACCTTCTCACGGACGGCGTCGATCACCGCTTCGTTGTTCAGGTCGGCCAGGATGTCg               >  3:8292/1‑145 (MQ=255)
                                                                                                                                         cccGGCACTGGGCCGGGCTCTTTCACTTTAGGCAAGCGCCAATCAGTTGCCGTAAACCGGCAGCTTCTTGCAGATGGCCTTGACCTTCTCACGGACGGCGTCGATCACCGCTTCGTTGTTCAGGTCGGCCa                       <  6:201512/131‑1 (MQ=255)
                                                                                                                                         cccGGCACTGGGCCGGGCTCTTTCACTTTAGGCAAGCGCCAATCAGTTGCCGTAAACCGGCAGCTTCTTGCAGATGGCCTTGACCTTCTCACGGACGGCGTCGATCACCGCTTCGTTGTTCAGGTCGGCCa                       >  5:201512/1‑131 (MQ=255)
                                                                                                                                            ggCACTGGGCCGGGCTCTTTCACTTTAGGCAAGCGCCAATCAGTTGCCGTAAACCGGCAGCTTCTTGCAGATGGCCTTGACCTTCTCACGGACGGCGTCGATCACCGCTTCGTTGTTCAGGTCGGCCAGGATGTCGCAGATCCAGCCgg  >  5:152177/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                             
CCAGTACGGCGGCGAAGTAGATGGGGATCTGCCGGGTTTCGAGTTGCTCTCTGGTCAAGGTGCGGCCTGTGCAAAGTGGAGGTATTGGGGGCGCAGTCTTTGTACGAGGGGCGGTGCGCCTGTATTAAACGAAAGAGCCCGGCACTGGGCCGGGCTCTTTCACTTTAGGCAAGCGCCAATCAGTTGCCGTAAACCGGCAGCTTCTTGCAGATGGCCTTGACCTTCTCACGGACGGCGTCGATCACCGCTTCGTTGTTCAGGTCGGCCAGGATGTCGCAGATCCAGCCGG  >  NC_002947/780413‑780701

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑