| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 812,329 | A→G | 16.7% | intergenic (‑78/‑150) | PP_0698 ← / → PP_0699 | LysR family transcriptional regulator/LysE family transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 812,329 | 0 | A | G | 16.7% | 79.7 / 9.1 | 36 | intergenic (‑78/‑150) | PP_0698/PP_0699 | LysR family transcriptional regulator/LysE family transporter |
| Reads supporting (aligned to +/- strand): ref base A (16/14); new base G (4/2); total (20/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.72e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.29e-01 | |||||||||||
GCGCGCACGAAGCTCTCGGTCTGGGCGGCAATGTCGAAGTAGCGGAAGGCGTTGAGTGGCGGCAGTTTCATGATATCCCTGTTCAGCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACATGGCCACATATTTCCCTGTAGGGGCAGGTTTACCCGCGAAGAGGCCAAGGCAGGCTGTACATATCTGTGAGCAAAACTATCAGATTCATCTCAGCATAAATCGATTTTTCCGCATCTGTAAGCGCTTGATAATCGGCGGTCTGTGCATTTCCATCGCCTATCGAGAGCCCTCA > NC_002947/812181‑812476 | gcgcgcACGAAGCTCTCGGTCTGGGCGGCAATGTCGAAGTAGCGGAAGGCGTTGATTGGCGGCATTTTCATGATATCCCTGTTCAGCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACATGGCCACATATTTCCCTGTAGGGGCa < 5:250744/149‑1 (MQ=255) gAAGCTCTCGCTCTGGGCGGCAATGTCGAAGTAGCGGAAGGCGTTGAGTGGCGGCAGTTTCATGATATCCCTGTTCAGCTGTGCCGGCCTATTCGCGGGTAAACCCGCTCCTACATGGCCACATATTTCCCTGTAGGGGCAGGTTTAcc < 5:228260/149‑1 (MQ=255) aaGCTCTCGGTCTGGGCGGCAATGTCGAAGTAGCGGAAGGCGTTGAGTGGCGGCAGTTTCATGATATCCCTGTTCAGCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACATGGCCACATATTTCCCTGTAGGGGCAGGTTTAccc < 6:27207/149‑1 (MQ=255) aaGCGCTCGGTCTGGGCGGCAATGTCGAAGTAGCGGAAGGCGTTGAGTGGCGGCAGTTTCATGATATCCCTGTTCAGCTGTGCCGGCCTCTTCGCGGGTAAACCTGCCCCTACAGGGAAATATGTGGCCATGTAGGAGCGGGTTTAccc < 4:140910/149‑1 (MQ=18) cggcAATGTCGAAGTAGCGGAAGGCGTTGAGTGGCGGCAGTTTCATGATATCCCTGTTCAGCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACATGGCCACATATTTCCCTGTAGGGGCAGGTTTACCCGCGAAGAgg < 5:23028/142‑1 (MQ=255) tCGAAGTAGCGGAAGGCGTTGAGTGGCGGCAGTTTCATGATATCCCTGTTCAGCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACATGGCCAAATATTTCCCTGTAGGGGCAGGTTTAACCGCGAAGAGGCCAAGGCAGGCTGt > 7:71961/1‑148 (MQ=255) gAAGTAGCGGAAGGCGTTGAGTGTCGGCAGTTTCATGATATCCCTGTTCAGCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACATGGCCACATATTTCCCTGTAGGGGCAGGTTTACCCGCGAAGAGGCCAAGGCAGGCTGTAc < 4:125413/148‑1 (MQ=255) cGGAAGGCGTTGAGTGGCGGCAGTTTCATGATATCCCTGTTCAGCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACATGGCCACATATTTACCTGTAGGGGCAGGTTTACCCGCGAAGAGGCCAAGGCAGGCTGTACATATCtgt > 4:147810/1‑149 (MQ=255) tCCCTGTTCAGCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACATGGCCACATATTTCCCTGTAGGGGCAGGTTTACCCGCGAAGAGGCCAAGGCAGGCTGTACATATCTGTGAGCAAAACTATCAGATTCATCTCAGCATAAAt < 3:147810/149‑1 (MQ=255) cccTGTTCAGCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACATGGCCACATATTTCCCTGTAGGGGCAGGTTTACCCGCGAAGAGGCCAAGGCAGGCTGTACATATCTGTGAGCAAAACTATCAGATTCATCTCAGCATAAATc > 5:200951/1‑149 (MQ=255) aGCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACATGGCCACATATTTCCCTGTAGGGGCAGGTTTACCCGCGAAGAGGCCAAGGCAGGCTGTACATATCTGTGAGCAAAACTATCAGATTCATCTCAGCATAAATCGATTTTTc > 3:200004/1‑149 (MQ=255) gCTGTGCCGGCCTCTTCGCGGGTTAACAGGCTCCTACTTGGCCACATATTTCCCTGTTGGGGCAGGTTTACCCGCTAAGAGGCCAAGGCAGGCTGTACATATCTGTGAGCAAAACTATCAGATTCATCTCAGCATAAATCGATTTTTcc < 4:93981/149‑1 (MQ=255) cTGTGCCGGCCTGTTCGCGGGTATACCCTCTCCTACATGGCCTCATTTTTCCCTGTAGGGGCAGGTTTACCAGCGAAGAGGCCAAGGCAGGCTGTAGATATCTGTGAGCAAAACTATCAGATTCATCTCAGCATAAATCGATTTTTCCg < 3:40456/149‑1 (MQ=255) tgCCGGCCTGTTCGCGGGTAACCCCGCTCCTACATGGCCACATATTTCCCTGTAGGGGCAGGTTTACCCGCGAAGAGGCCAAGGCAGGCTGTACATATCTGTGAGCAAAACTATCAGATTCATCTCAGCATAAATCGATTTTTCCGCAt < 7:90020/149‑1 (MQ=255) ccGGCCTCTTCGCGGGTAAACCCGCTCCTACATGGCCACATATTTCCCTGTAGGGGCAGGTTTACCCGCGAAGAGGCCAAGGCAGGCTGTACATATCTGTGAGCAAAACTATCAGATTCATCTCAGCATAAATCGAGTTTTCCGCATCt > 7:172164/1‑149 (MQ=255) ccGGCCTCTTCGCGGGTAAACCCGCTCCTACATGGCCACATATTTACCTGTAGGGGCAGGTTTACCCGCGAAGAGGCCAAGGCAGGCTGTACATATCTGTGAGCAAAACTATCAGATTCATCTAAGGATAAATCGATTTTTCCGCATCt > 7:216302/1‑149 (MQ=255) gCCTCTTCGCGGGTAAACCCGCTCCTACATGGCCACATATTTCCCTGTAGGGGCAGGTTTACCCGCGAAGAGGCCAAGGCAGGCTGTACATATCTGTGAGCAAAACTATCAGATTCATCTCAGCATAAATCGATTTTTCCGCATCTGt > 7:165658/1‑148 (MQ=255) ttCGCGGGTAAACCCGCTCCTACATGGCCACATATTTCCCTGTAGGGGCAGGTTTACCCGCGAAGAGGCCAAGGCAGGCTGTACATATCTGTGAGCAAAACTATCAGATTCATCTCAGCATAAATCGATTTTTCCGCATCTGTAAgcgc > 6:69895/1‑149 (MQ=255) ttCGCGGGTAAACCCGCTCCTACATGGCCACATATTTCCCTGTAGGGGCAGGTTTACCCGCGAAGAGGCCAAGGCAGGCTGTACATATCTGTGAGCAAAACTATCAGATTCATCTCAGCATAAATCGATTTTTCCGCATCTGTAAgc < 8:71961/147‑1 (MQ=255) cgcgGGTAAACCCGCTCCTACATGGCCACATATTTCCCTGTAGGGGCAGGTTTACCCGCGAAGAGGCCAAGGCAGGCTGTACATATCTGTGAGCAAAACTATCAGATTCATCTCAGCATAAATCGATTTTTCCGCATCTGTAAGCGCt < 8:165658/148‑1 (MQ=255) gggTAAACCTGCCCCTACAGGGAAATATGTGGCCATGTAGGAGCGGGTTTACCCGCGAAGAGGCCAAGGCAGGCTGTACATATCTGTGAGCAAAACTATCAGATTCATCTCAGCATAAATCGATTTTTCCGCATCTGTAAGCGCTTGa > 5:168970/1‑148 (MQ=21) gggTAAACCTGCCCCTACAGGGAAATATGTGGCCATGTAGGAGCGGGTTTACCCGCGAAGAGGCCAAGGCAGGCTGTACATATCTGTGAGCAAAACTATCAGATTCATCTCAGCATAAATCGATTTTTCCGCATCTGTAAGCGCTTGa > 6:52517/1‑148 (MQ=21) gggTAAACCTGCCCCTACAGGGAAATATGTGGCCATGTAGGAGCGGGTTTACCCGCGAAGAGGCCAAGGCAGGCTGTACATATCTGTGAGCAAAACTATCAGATTCATCTCAGCATAAATCGATTTTTCCGCATCTGTAAGCGCTTGa > 6:148687/1‑148 (MQ=21) cccGCTGGTACATGGCCAGATAGTTCCGTGTAGGGGCAGGTTTACCCGCGAAGAGGCCAAGGCAGGCTGTACATATCTGTGAGCAAAGCTATCAGATTCATCTCAGCATTAATCGATTTGTCGGCATCTGTATGCGCTTGATATTcggg > 2:168412/1‑148 (MQ=255) ccGCTCCTACATGGCCACATATTTCCCTGTAGGGGCAGGTTTACCCGCGAAGAGGCCAAGGCAGGCTGTACATATCTGTGAGCAAAACTATCAGATTCATCTCAGCATAAATCGATTTTTCCGAATCTGTAAGCGCTTGATAATcggcg > 8:89412/1‑149 (MQ=255) cTACATGGCCACATATTTCCCTGTAGGGGCAGGTTTACCCGCGAAGAGGCCAAGGCAGGCTGTACATATCTGTGAGCAAAACTATCAGATTCATCTCAGCATAAATCGATTTTTCCGCATCTGTAAGCGCTTGATAATCGGCGGTCtgt > 7:115296/1‑149 (MQ=255) cTACATGGCCACATATTTCCCTGTAGGGGCAGGTTTACCCGCGAAGAGGCCAAGGCAGGCTGTACATATCTGTGAGCAAAACTATCAGATTCATCTCAGCATAAATCGATTTTTCCGCATCTGTAAGCGCTTGATAATCGGCGGTCt < 5:69895/147‑1 (MQ=255) aCATGGCCACATATTTCCCTGTAGGGGCAGGTTTACCCGCGAAGAGGCCAAGGCAGGCTGTACATATCTGTGAGCAAAACTATCAGATTCATCTCAGCATAAATCGATTTTTCCGCATGTGTATGCGCTTGATAATCGGCGGTCTGTGc > 2:72332/1‑149 (MQ=255) ccACATATTTCCCTGTAGGGGCAGGTTTACCCGCGAAGAGGCCAAGGCAGGCTGTACATATCTGTGAGCAAAACTATCAGATTCATCTCAGCATAAATCGATTTTTCCGCATCTGTAAGCGCTTGATAATCGGCGGTCTGTGCATTTc > 6:627/1‑148 (MQ=255) gccatGTAGGAGCGGGTTTACCCGCGAAGAGGCCAAGGCAGGCTGTACATATCTGTGAGCAAAACTATCAGATTCATCTCAGCATAAATCGATTTTTCCGCATCTGTAAGCGCTTGa < 2:207564/113‑1 (MQ=255) gccatGTAGGAGCGGGTTTACCCGCGAAGAGGCCAAGGCAGGCTGTACATATCTGTGAGCAAAACTATCAGATTCATCTCAGCATAAATCGATTTTTCCGCATCTGTAAGCGCTTGa > 1:207564/5‑117 (MQ=255) gTAGGGGCAGGTTTACCCGCGAAGAGGCCAAGGCAGGCTGTACATATCTGTGAGCAAAACTATCAGATTCATCTCAGCATAAATCGATTTTTc < 4:24288/93‑1 (MQ=255) gTAGGGGCAGGTTTACCCGCGAAGAGGCCAAGGCAGGCTGTACATATCTGTGAGCAAAACTATCAGATTCATCTCAGCATAAATCGATTTTTc > 3:24288/1‑93 (MQ=255) tAGGGGCAGGTTTACCCGCGAAGAGGCCAAGGCAGGCTGTACATATCTGTGAGCAAAACTATCAGATTCATCTCAGCATAAATCGATTTTTCCGCATCTGTAAGCGCTTGATAATCGGCGGTCTGTGCATTTCCATCGCCTATCgaga < 8:115296/148‑1 (MQ=255) gggCAGGTTTACCCGCGAAGAGGCCAAGGCAGGCTGTACATATCTGTGAGCAAAACTATCAGATTCATCTCAGCATAAATCGATTTTTCCGCATCTGTAAGCGCTTGATAATCGGCGGTCTGTGCATTTCCATCGCCTATCGAGAGccc > 5:265580/1‑149 (MQ=255) ggCAGGTTTACCCGCGAAGAGGCCAAGGCAGGCTGTACATATCTGTGAGCAAAACTATCAGATTCATCTCAGCATAAATCGATTTTTCCGCATCTGTAAGCGCTTGATAATCGGCGGTCTGTGCATTTCCATAGCCTATCGAGAGCCCt > 5:103881/1‑149 (MQ=255) cAGGTTTACCCTCGAAGAGGCCAAGGCAGGCTGTACATATCTGTGAGCAAAACTATCAGATTCATCTCAGCATAAATCGATTTTTCCGCATCTGTAAGCGCTTGATAATCGGCGGTCTGTGCATTTCCATCGCCTATCGAGAGCCCTCa < 3:42651/149‑1 (MQ=255) | GCGCGCACGAAGCTCTCGGTCTGGGCGGCAATGTCGAAGTAGCGGAAGGCGTTGAGTGGCGGCAGTTTCATGATATCCCTGTTCAGCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACATGGCCACATATTTCCCTGTAGGGGCAGGTTTACCCGCGAAGAGGCCAAGGCAGGCTGTACATATCTGTGAGCAAAACTATCAGATTCATCTCAGCATAAATCGATTTTTCCGCATCTGTAAGCGCTTGATAATCGGCGGTCTGTGCATTTCCATCGCCTATCGAGAGCCCTCA > NC_002947/812181‑812476 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |