Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 820,150 G→A 7.4% A210A (GCG→GCA)  ada → fused O(6)‑alkylguanine DNA alkyltransferase/transcriptional activator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947820,1500GA 7.4% 160.0 / 2.3 54A210A (GCG→GCA) adafused O(6)‑alkylguanine DNA alkyltransferase/transcriptional activator
Reads supporting (aligned to +/- strand):  ref base G (26/24);  new base A (2/2);  total (28/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.14e-01

CCGCTTCTACGAAAGTGCCGACCAGCGCCTGGGCATGCGCCCCCGGCAATACCGCGCCGGCGGTGCCGGGGCAACCATTCACTTCGCCCTTGGGCAATGCTCGCTGGGTGCAATACTGGTAGCCCAGAGCGACAAGGGCATCTGCGCGATTCTGCTGGGCGACGACCCCGAGGGCTTGTTGCACGAGCTGCAGGATCAGTTCCCCAAGGCCCACCTGATTGGCGGCGACAGCGCCTACGAGCAGAAGGTCGCCGAAGTGATCGGTTTCGTCGAAGCGCCCTCCTTGGGCCTGGCCC  >  NC_002947/820003‑820298
                                                                                                                                                   |                                                                                                                                                    
ccGCTTCTACGAAAGTGCCGACCAGCGCCTGTGCATGCGCCCCCGGCAATACCGCGCCGGCGGTGCCGGGGCAACCATTCACTTCGCCCTTGGGCAATGCTCGCTGGGTGCAATACTGGTAGCCCAGAGCGACAAGGGCATCTGCGCGa                                                                                                                                                     <  6:47654/149‑1 (MQ=255)
          gAAAGTGCCGACCAGCGCCTGGGCATGCGCCCCCGGCAATACCGCGCCGGCGGTGCCGGGGCAACCATTCACTTCGCCCTTGGGCAATGCTCGCTGGGTGCAATACTGGTAGCCCAGAGCGACAAGGGCATCTGCGCGATTCTGCTggg                                                                                                                                           >  6:161049/1‑149 (MQ=255)
            aaGTGCCGACCAGCGCCTGGGCATGCGCCCCCGGCAATACCGCGCCGGCGGTGCCGGGGCAACCATTCACTTCGCCCTTGGGCAATGCTCGCTGGGTGCAATACTGGTAGCCCAGAGCGACAAGGGCATCTGCGCGATTCTGCTGcgcg                                                                                                                                         >  1:170737/1‑149 (MQ=255)
               tGCCGCCCAGCGCCTGGGCATGCGCCCCCGGCAATACCGCGCCGGCGGTGCCGGGGCAACCATTCACTTCGCCGTTGGGCAATGCTCGCTGGGTGCAATACTGGTAGCCCAGAGCGACAAGGGCATCTGCGCGATTCTGCTGGGcgacg                                                                                                                                      >  6:136474/1‑149 (MQ=255)
               tGCCGACCAGCGCCTGGGCATGCGCCCCCGGCAATACCGCGCCGGCGGTGCCGGGGCAACCATTCACTTCGCCCTTGGGCAATGCTCGCTGGGTGCAATACTGGTAGCCCAGAGCGACAAGGGCATCTGCGCGATTCTGCTGGGcgacg                                                                                                                                      <  3:187333/149‑1 (MQ=255)
                    aCCAGCGCCTGGGCATGCGCCCCCGGCAATACCGCGCCGGCGGTGCCGGGGCAACCATTCACTTCGCCCTTGGGCAATGCTCGCTGGGTGCAATACTGGTAGCCCAGAGCGACAAGGGCATCTGCGCGATTCTGCTGGGCGACGAcccc                                                                                                                                 <  5:161049/149‑1 (MQ=255)
                       aGCGCCTGGGCATGCGCCCCCGGCAATACCGCGCCGGCGGTGCCGGGGCAACCATTCACTTCGCCCTTGGGCAATGCTCGCTGGGTGCAATACTGGTAGCCCAGAGCGACAAGGGCATCTGCGCGATTCTGCTGGGCGACGACCCCGAg                                                                                                                              <  1:268759/149‑1 (MQ=255)
                       aGCGCCTGGGCATGCGCCCCCGGCAATACCGCGCCGGCGGTGCCGGGGCAACCATTCACTTCGCCCTTGGGCAATGCTCGCTGGGTGCAATACTGGTAGCCCAGAGCGACAAGGGCATCTGCGCGATTCTGCTGGGCGACGACCCCGAg                                                                                                                              >  2:163165/1‑149 (MQ=255)
                           ccTGGGCATGCGCCCCCGGCAATACCGCGCCGGCGGTGCCGGGGCAACCATTCACTTCGCCCTTGGGCAATGCTCGCTGGGTGCAATACTGGTAGCCCAGAGCGACAAGGGCATCTGCGCGATTCTGCTGGGCGACGACCCCGAGGGCt                                                                                                                          >  2:126300/1‑149 (MQ=255)
                            cTGGGCATGCGCCCCCGGCAATACCGCGCCGGCGGTGCCGGGGCAACCATTCACTTCGCCCTTGGGCAATGCTCGCTGGGTGCAATACTGGTAGCCCAGAGCGACAAGGGCATCTGCGCGATTCTGCTGGGCGACGACCCCGAGGGCtt                                                                                                                         >  4:234758/1‑149 (MQ=255)
                              gggCATGCGCCCCCGGCAATACCGCGCCGGCGGTGCCGGGGCAACCATTCACTTCGCCCTTGGGCAATGCTCGCTGGGTGCAATACTGGTAGCCCAGAGCGACAAGGGCATCTGCGCGATTCTGCTGGGCGACGACCCCGAGGGCttgt                                                                                                                       >  7:119997/1‑149 (MQ=255)
                                       cccccGGCAATACCGCGCCGGCGGTGCCGGGGCAACCATTCACTTCGCCCTTGGGCAATGCTCGCTGGGTGCAATACTGGTAGCCCAGAGCGACAAGGGCATCTGCGCGATTCTGCTGGGCGACGACCCCGAGGGCTTGTTGCACGAGc                                                                                                              >  8:100762/1‑149 (MQ=255)
                                          ccGGCAATACCGCGCCGGCGGTGCCGGGGCAACCATTCACTTCGCCCTTGGGCAATGCTCGCTGGGTGCAATACTGGTAGCCCAGAGCGACAAGGGCATCTGCGCGATTCTGCTGGGCGACGACCCCGAGGGCTTGTTGCACGAGCTGc                                                                                                           >  3:126957/1‑149 (MQ=255)
                                           cgtcAATACCGCGCCGGCGGTGCCGGGGCAACCATTCACTTCGCCCTTGGGCAATGCTCGCTGGGTGCAATACTGGTAGCCCAGAGCGACAAGGGCATCTGCGCGATTCTGCTGGGCGACGACCCCGAGGGCTTGTTGCACGAGCTGCa                                                                                                          <  8:239952/146‑1 (MQ=255)
                                           cGGCAATACCGCGCCGGCGGTGCCGGGGCAACCATTCACTTCGCCCTTGGGCAATGCTCGCTGGGTGCAATACTGGTAGCCCAGAGCGACAAGGGCATCTGCGCGATTCTGCTGGGCGACGACCCCGAGGGCTTGTTGCACGAGCTGCa                                                                                                          <  7:100762/149‑1 (MQ=255)
                                                   ccGCGCCGGCGGTGCCGGGGCAACCATTCACTTCGCCCTTGGGCAATGCTCGCTGGGTGCAATACTGGTAGCCCAGAGCGACAAGGGCATCTGCGCGATTCTGCTGGGCGACGACCCCGAGGGCTTGTTGCACGAGCTGCAGGATCAg                                                                                                   <  1:38858/148‑1 (MQ=255)
                                                   ccGCGCCGGCGGTGCCGGGGCAACCATTCACTTCGCCCTTGGGCAATGCTCGCTGGGTGCAATACTGGTAGCCCAGAGCGACAAGGGCATCTGCGCGATTCTGCTGGGCGACGACCCCGAGGGCTTGTTGCACGAGCTGCAGGATCAg                                                                                                   <  2:30037/148‑1 (MQ=255)
                                                     gcgcCGGCGGTGCCGGGGCAACCATTCACTTCGCCCTTGGGCAATGCTCGCTGGGTGCAATACTGGTAGCCCAGAGCGACAAGGGCATCTGCGCGATTCTGCTGGGCGACGACCCCGAgg                                                                                                                             >  4:225527/1‑120 (MQ=255)
                                                     gcgcCGGCGGTGCCGGGGCAACCATTCACTTCGCCCTTGGGCAATGCTCGCTGGGTGCAATACTGGTAGCCCAGAGCGACAAGGGCATCTGCGCGATTCTGCTGGGCGACGACCCCGAgg                                                                                                                             <  3:225527/120‑1 (MQ=255)
                                                           gcggTGCCGGGGCAACCATTCACTTCGCCCTTGGGCAATGCTCGCTGGGTGCAATACTGGTAGCCCAGAGCGACAAGGGCATCTGCGCGATTCTGCTGGGCGACGACCCCGAGGGCTTGTTGCACGAGCTGCAGGATCAGTTCCCCAAg                                                                                          <  1:126300/149‑1 (MQ=255)
                                                            cggTGCCGGGGCAACCATTCACTTCGCCCTTGGGCAATGCTCGCTGGGTGCAATACTGGTAGCCCAGAGCGACAAGGGCATCTGCGCGATTCTGCTGGGCGACGACCCCGAGGGCTTGTTGCACGAGCTGCAGGATCAGTTCCCCAAgg                                                                                         <  8:119997/149‑1 (MQ=255)
                                                                          ccATTCACTTCGCCCTTGGGCAATGCTCGCTGGGTGCAATACTGGTAGCCCAGAGCGACAAGGGCATCTGCGCGATTCTGCTGGGCGACGACCCCGAGGGCTTGTTGCACGAGCTGCAGGATCAGTTCCCCAAGGCCCACCTGATTggc                                                                           >  3:76960/1‑149 (MQ=255)
                                                                           cATTCACTTCGCCCTTGGGCAATGCTCGCTGGGTGCAATACTGGTAGCCCAGAGCGACAAGGGCATCTGCGCGATTCTGCTGGGCGACGACCCCGAGGGCTTGTTGCACGAGCTGCAGGATCAGTTCCCCAAGGCCCACCTGATTggcg                                                                          >  3:131852/1‑149 (MQ=255)
                                                                                aCTTCGCCCTTGGGCAATGCTCGCTGGGTGCAATACTGGTAGCCCAGAGCGACAAGGGCATCTGCGCGATTCTGCTGGGCGACGACCCCGAGGGCTTGTTGCACGAGCTGCAGGATCAGTTCCCCAAGGCCCACCTGATTggcg                                                                          >  7:6886/1‑144 (MQ=255)
                                                                                aCTTCGCCCTTGGGCAATGCTCGCTGGGTGCAATACTGGTAGCCCAGAGCGACAAGGGCATCTGCGCGATTCTGCTGGGCGACGACCCCGAGGGCTTGTTGCACGAGCTGCAGGATCAGTTCCCCAAGGCCCACCTGATTggcg                                                                          <  8:6886/144‑1 (MQ=255)
                                                                                aCTTCGCCCTTGGGCAATGCTCGCTGGGTGCAATACTGGTAGCCCAGAGCGACAAGGGCATCTGCGCGATTCTGCTGGGCGACGACCCCGAGGGCTTGTTGCACGAGCTGCAGGATCAGTTCCCCAAGGCCCACCTGATTGGCGGCGa                                                                      >  2:131694/1‑148 (MQ=255)
                                                                                aCTTCGCCCTTGGGCAATGCTCGCTGGGTGCAATACTGGTAGCCCAGAGCGACAAGGGCATCTGCGCGATTCTGCTGGGCGACGACCCCGAGGGCTTGTTGCACGAGCTGCAGGATCAGTTCCCCAAGGCCCACCTGATTGGCGGCGa                                                                      >  5:174108/1‑148 (MQ=255)
                                                                                 cTTCGCCCTTGGGCAATGCTCGCTGGGTGCAATACTGGTAGCCCAGAGCGACAAGGGCATCTGCGCGATTCTGCTGGGCGACGACCCCGAGGGCTTGTTGCACGAGCTGCAGGATCAGTTCCCCAAGGCCCACCTGATTGGCGGCGa                                                                      >  8:156211/1‑147 (MQ=255)
                                                                                 cTTCGCCCTTGGGCAATGCTCGCTGGGTGCAATACTGGTAGCCCAGAGCGACAAGGGCATCTGCGCGATTCTGCTGGGCGACGACCCCGAGGGCTTGTTGCACGAGCTGCAGGATCAGTTCCCCAAGGCCCACCTGATTGGCGGCGa                                                                      >  2:225717/1‑147 (MQ=255)
                                                                                     gCCCTTGGGCAATGCTCGCTGGGTGCAATACTGGTAGCCCAGAGCGACAAGGGCATCTGCGCGATTCTGCTGGGCGACGACCCCGAGGGCTTGTTGCACGAGCTGCAGGATCAGTTCCCCAAGGCCCACCTGATTGGCGGCGACAgcg                                                                 <  5:136474/148‑1 (MQ=255)
                                                                                            ggCAATGCTCGCTGGGTGCAATACTGGTAGCCCAGAGCGACAAGGGCATCTGCGCGATTCTGCTGGGCGACGACCCCGAGGGCTTGTTGCACGAGCTGCAGGATCAGTTCCCCAAGGCCCACCTGATTGGCGGCGACAGCGCCTACGa                                                          <  4:76960/148‑1 (MQ=255)
                                                                                              cAATGCTCGCTGGGTGCAATACTGGTAGCCCAGAGCGACAAGGGCATCTGCGCGATTCTGCTGGGCGACGACCCCGAGGGCTTGTTGCACGAGCTGCAGGATCAGTTCCCCAAGGCCCACCTGATTGGCGGCGACAGCGCCTACGAg                                                         >  4:2588/1‑147 (MQ=255)
                                                                                                aTGCTCGCTGGGTGCAATACTGGTAGCCCAGAGCGACAAGGGCATCTGCGCAATTCTGCTGGGCGTCGACCCCGAGGGCTTGTTGCACGAGCTGCa                                                                                                          <  1:23116/96‑1 (MQ=255)
                                                                                                aTGCTCGCTGGGTGCAATACTGGTAGCCCAGAGCGACAAGGGCATCTGCGCAATTCTGCTGGGCGACGACCCCGAGGGCTTGTTGCACGAGCTGCa                                                                                                          >  8:204121/1‑96 (MQ=255)
                                                                                                aTGCTCGCTGGGTGCAATACTGGTAGCCCAGAGCGACAAGGGCATCTGCGCAATTCTGCTGGGCGACGACCCCGAGGGCTTGTTGCACGAGCTGCa                                                                                                          <  7:204121/96‑1 (MQ=255)
                                                                                                aTGCTCGCTGGGTGCAATACTGGTAGCCCAGAGCGACAAGGGCATCTGCGCAATTCTGCTGGGCGACGACCCCGAGGGCTTGTTGCACGAGCTGCa                                                                                                          >  2:23116/1‑96 (MQ=255)
                                                                                                   cTCGCTGGGTGCAATACTGGTAGCCCAGAGCGACAAGGGCATCTGCGCGATTCTGCTGGGCGACGACCCCGAGGGCTTGTTGCACGAGCTGCAGGATCAGTTCCCCAAGGCCCACCTGATTGGCGGCGACAGCGCCTACGAGCAGAAg                                                   <  1:163165/148‑1 (MQ=255)
                                                                                                       cTGGGTGCAATACTGGTAGCCCAGAGCGACAAGGGCATCTGCGCGATTCTGCTGGGCGACGACCCCGAGGGCTTGTTGCACGAGCTGCAGGATCAGTTCCCCAAGGCCCACCTGATTGGCGGCGACAGCGCCTACGAGCAGAAGGTCGc                                              >  1:72786/1‑149 (MQ=255)
                                                                                                          ggTGCAATACTGGTAGCCCAGAGCGACAAGGGCATCTGCGCGATTCTGCTGGGc                                                                                                                                          >  8:65666/1‑54 (MQ=255)
                                                                                                          ggTGCAATACTGGTAGCCCAGAGCGACAAGGGCATCTGCGCGATTCTGCTGGGc                                                                                                                                          <  7:65666/54‑1 (MQ=255)
                                                                                                          ggTGCAATACTGGTAGCCCAGAGCGACAAGGGCATCTGCGCGATTCTGCTGGGCGACGACCCCGAGGGCTTGTTGCACGAGCTGCAGGATCAGTTCCCCAAGGCCCACCTGATTGGCGGCGACAGCGCCTACGAGCAGAAGGTCGCCg                                            >  3:257896/1‑148 (MQ=255)
                                                                                                               aaTACTGGTAGCCCAGAGCGACAAGGGCATCTGCGCGATTCTGCTGGGCGACGACCCCGAGGGCTTGTTGCACGAGCTGCAGGAt                                                                                                      <  4:142461/85‑1 (MQ=255)
                                                                                                               aaTACTGGTAGCCCAGAGCGACAAGGGCATCTGCGCGATTCTGCTGGGCGACGACCCCGAGGGCTTGTTGCACGAGCTGCAGGAt                                                                                                      >  3:142461/1‑85 (MQ=255)
                                                                                                               aaTACTGGTAGCCCAGAGCGACAAGGGCATCTGCGCGATTCTGCTGGGCGACGACCCCGAGGGCTTGTTGCACGAGCTGCAGGATCAGTTCCCCAAGGCCCACCTGATTGGCGGCGACAGCGCCTACGAGCAGAAGGTCGCCGAAGTGa                                      >  3:83358/1‑149 (MQ=255)
                                                                                                               aaTACTGGTAGCCCAGAGCGACAAGGGCATCTGCGCGATTCTGCTGGGCGACGACCCCGAGGGCTTGTTGCACGAGCTGCAGGATCAGTTCCCCAAGGCCCACCTGATTGGCGGCGACAGCGCCTACGAGCAGAAGGTCGCCGAAGTGa                                      >  7:12935/1‑149 (MQ=255)
                                                                                                                 tACTGGTAGCCCAGAGCGACAAGGGCATCTGCGCGATTCTGCTGGGCGACGACCCCGAGGGCTTGTTGCACGAGCTGCAGGATCAGTTCCCCAAGGCCCACCTGATTGGCGGCGACAGCGCCTACGAGCAGAAGGTCGCCGAAGTGATc                                    <  4:257896/149‑1 (MQ=255)
                                                                                                                       tAGCCCAGAGCGACAAGGGCATCTGCGCGATTCTGCTGGGCGACGACCCCGAGGGCTTGTTGCACGAGCTGCAGGATCAGTTCCCCAAGGCCCACCTGATTGGCGGCGACAGCGCCTACGAGCAGAAGGTCGCCGAAGTGATCGGTTtc                              >  2:238766/1‑149 (MQ=255)
                                                                                                                          cccAGAGCGACAAGGGCATCTGCGCGATTCTGCTGGGCGACGACCCCGAGGGCTTGTTGCACGAGCTGCAGGATCAGTTCCCCAAGGCCCACCTGATTGGCGGCGACAGCGCCTACGAGCAGAAGGTCGCCGAAGTGATCGGTTtcgtc                           <  3:234758/149‑1 (MQ=255)
                                                                                                                               agCGACAAGGGCATCTGCGCGATTCTGCTGGGCGACGACCCCGAGGGCTTGTTGCACGAGCTGCAGGATCAGTTCCCCAAGGCCCACCTGATTGGCGGCGACAGCGCCTACGAGCAGAAGGTCGCCGAAGTGATCGGTTTCGTCGAAgc                      <  8:12935/149‑1 (MQ=255)
                                                                                                                                  gACAAGGGCATCTGCGCGATTCTGCTGGGCGACGACCGCGAGGGCTTGTTGCACGAGCTGCAGGATCAGTTCCCGAAGGCCCACCTGATTGGCGGCGACAGCGCCTACGAGCAGAAGGTCGCCGAAGTGATCGGTTTCGTCGAAGCGcc                   <  2:170737/149‑1 (MQ=255)
                                                                                                                                   aCAAGGGCATCTGCGCGATTCTGCTGGGCGACGACCCCGAGGGCTTGTTGCACGAGCTGCAGGATCAGTTCCCCAAGGCCCACCTGATTggcggc                                                                        <  8:74221/95‑1 (MQ=255)
                                                                                                                                   aCAAGGGCATCTGCGCGATTCTGCTGGGCGACGACCCCGAGGGCTTGTTGCACGAGCTGCAGGATCAGTTCCCCAAGGCCCACCTGATTggcggc                                                                        >  7:74221/1‑95 (MQ=255)
                                                                                                                                         gCATCTGCGCGATTCTGCTGGGCGACGACCCCGAGGGCTTGTTGCACGAGCTGCAGGATCAGTTCCCCAAGGCCCACCTGATTGGCGGCGACAGCGCCTACGAGCAGAAGGTCGCCGAAGTGATCGGTTTCGTCGAAGCGCCCTCCTTg            <  4:131852/149‑1 (MQ=255)
                                                                                                                                         gCATCTGCGCGATTCTGCTGGGCGACGACCCCGAGGGCTTGTTGCACGAGCTGCAGGATCAGTTCCCCAAGGCCCACCTGATTGGCGGCGACAGCGCCTACGAGCAGAAGGTCGCCGAAGTGATCGGTTTCGTCGAAGCGCCCTCCTTg            <  1:131694/149‑1 (MQ=255)
                                                                                                                                                cgcgATTCTGCTGGGCGACGACCCCGAGGGCTTGTTGCACGAGCTGCAGGATCAGTTCCCCAAGGccc                                                                                      <  6:102452/68‑1 (MQ=255)
                                                                                                                                                cgcgATTCTGCTGGGCGACGACCCCGAGGGCTTGTTGCACGAGCTGCAGGATCAGTTCCCCAAGGCCCACCTGATTGGCGGCGACAGCGCCTACGAGCAGAAGGTCGCCGAAGTGATCGGTTTCGTCGAAGCGCCCTCCTTGGGc         <  3:2588/145‑1 (MQ=255)
                                                                                                                                                cgcgATTCTGCTGGGCGACGACCACGAGGGCTTGTTGCACGAGCTGCAGGATCAGTTCCCCAAGGccc                                                                                      >  5:102452/1‑68 (MQ=255)
                                                                                                                                                   gATTCTGCTGGGCGACGACCCCGAGGGCTTGTTGCACGAGCTGCAGGATCAGTTCCCCAAGGCCCACCTGATTGGCGGCGACAGCGCCTACGAGCAGAAGGTCGCCGAAGTGATCGGTTTCGTCGAAGCGCCCTCCTTGGGCCTGGccc  >  1:247422/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
CCGCTTCTACGAAAGTGCCGACCAGCGCCTGGGCATGCGCCCCCGGCAATACCGCGCCGGCGGTGCCGGGGCAACCATTCACTTCGCCCTTGGGCAATGCTCGCTGGGTGCAATACTGGTAGCCCAGAGCGACAAGGGCATCTGCGCGATTCTGCTGGGCGACGACCCCGAGGGCTTGTTGCACGAGCTGCAGGATCAGTTCCCCAAGGCCCACCTGATTGGCGGCGACAGCGCCTACGAGCAGAAGGTCGCCGAAGTGATCGGTTTCGTCGAAGCGCCCTCCTTGGGCCTGGCCC  >  NC_002947/820003‑820298

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑