| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 863,900 | T→A | 11.6% | intergenic (+17/+19) | hemH → / ← uraA | ferrochelatase/uracil permease |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 863,900 | 0 | T | A | 11.6% | 132.3 / 9.1 | 52 | intergenic (+17/+19) | hemH/uraA | ferrochelatase/uracil permease |
| Reads supporting (aligned to +/- strand): ref base T (23/23); new base A (3/3); total (26/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.28e-01 | |||||||||||
GAAGAGATAGGCATGCGTGGCAGCGAGCAGTTTGTCGAGGCGGGCGGCCAGGAGCTGGTGCTGGTGCCGTGCCTGAATGATCACCCGGAGTGGGTGAGGGTGTTGGCTGATATGTGTGAAAAGGCCTGAATCGAAGGGGCCGCTTTGCGG‑CCCCTTCCGACCGGTCATACATCCAGCTTCTTGTGCTTCCAGCTGTCATTACCCGGCAGAATCAGGTTCAGGGCAATAGCCACAATGGCGCACAGGGCGATGCCCTTCAGGCCCCAGTCATCCGGGCCGTCACCGCTGC > NC_002947/863755‑864043 | gAAGAGATAGGCATGCGTGGCAGCGAGCAGTTTGTCGAGGCGGGCGGCCAGGAGCTGGTGCTGGTGCCGTGCCTGAATGATCACCCGGAGTGGGTGAGGGTGTTGGCTGATATGTGTGAAAAGGCCTGAATCGAAGGGGCCGCTTTGCg < 3:4625/149‑1 (MQ=255) gagaTAGGCATGCGTGGCAGCGAGCAGTTTGTCGAGGCGGGCGGCCAGGAGCTGGTGCTGGTGCCGTGCCTGAATGATCACCCGGAGTGGGTGAGGGTGTTGGCTGATATGTGTGAAAAGGCCTGAATCGAAGGGGCCGCAAAGCGG‑cc < 3:23062/149‑1 (MQ=255) aGGCATGCGTGGCAGCGAGCAGTTTGTCGAGGCGGGCGGCCAGGAGCTGGTGCTGGTGCCGTGCCTGAATGATCACCCGGAGTGGGTGAGGGTGTTGGCTGATATGTGTGAAAAGGCCTGAATCGAAGGGGCCGCTTTGCGG‑CCCCTTc > 7:138351/1‑149 (MQ=255) gCATGCGTGGCAGCGAGCAGTTTGTCGAGGCGGGCGGCCAGGAGCTGGTGCTGGTGCCGTGCCTGAATGATCACCCGGAGTGGGTGAGGGTGTTGGCTGATATGTGTGAAAAGGCCTGAATCGAAGGGGCCGCAAAGCGG‑CCCCTTc > 4:64936/1‑147 (MQ=255) gCATGCGTGGCAGCGAGCAGTTTGTCGAGGCGGGCGGCCAGGAGCTGGTGCTGGTGCCGTGCCTGAATGATCACCCGGAGTGGGTGAGGGTGTTGGCTGATATGTGTGAAAAGGCCTGAATCGAAGGGGCCGCAAAGCGG‑CCCCTTc < 3:64936/147‑1 (MQ=255) aTGCGTGGCAGCGAGCAGTTTGTCGAGGCGGGCGGCCAGGAGCTGGTGCTGGTGCCGTGCCTGAATGATCACCCGGAGTGGGTGAGGGTGTTGGCTGATATGTGTGAAAAGGCCTGAATCGAAGGGGCCGCTTTGCGG‑CCCCTTCCGAc > 5:231743/1‑149 (MQ=255) gAGCAGTTTGTCGAGGCGGGCGGCCAGGAGCTGGTGCTGGTGCCGTGCCTGAATGATCACCCGGAGTGGGTGAGGGTGTTGGCTGATATGTGTGAAAAGGCCTGAATCGAAGGGGCCGCTTTGCGG‑CCCCTTCCGACCGGTCATACATc > 8:204710/1‑149 (MQ=255) gAGCAGTTTGTCGAGGCGGGCGGCCAGGAGCTGGTGCTGGTGCCGTGCCTGAATGATCACCCGGAGTGGGTGAGGGTGTTGGCTGATATGTGTGAAAAGGCCTGAATCGAAGGGGCCGCTTTGCGG‑CCCCTTCCGACCGGTCATACATc < 1:93310/149‑1 (MQ=255) aGCAGTTTGTCGAGGCGGGCGGCCAGGAGCTGGTGCTGGTGCCGTGCCTGAATGATCACCCGGAGTGGGTGAGGGTGTTGGCTGATATGTGTGAAAAGGCCTGAATCGAAGGGGCCGCTTTGCGG‑CCCCTTCCGACCGGTCATACATcc < 2:63683/149‑1 (MQ=255) tttGTCGAGGCGGGCGGCCAGGAGCTGGTGCTGGTGCCGTGCCTGAATGATCACCCGGAGTGGGTGAGGGTGTTGGCTGATATGTGTGAAAAGGCCTGAATCGAAGGGGCCGCTTTGCGG‑CCCCTTCCGACCGGTCATACATCCAGctt > 5:232734/1‑149 (MQ=255) gAGGCGGGCGGCCAGGAGCTGGTGCTGGTGCCGTGCCTGAATGATCACCCGGAGTGGGTGAGGGTGTTGGCTGATATGTGTGAAAAGGCCTGAATCGAAGGGGCCGCAAAGCGG‑CCCCtt > 6:198995/1‑120 (MQ=255) gAGGCGGGCGGCCAGGAGCTGGTGCTGGTGCCGTGCCTGAATGATCACCCGGAGTGGGTGAGGGTGTTGGCTGATATGTGTGAAAAGGCCTGAATCGAAGGGGCCGCAAAGCGG‑CCCCtt < 5:198995/120‑1 (MQ=255) ggcggcCAGGAGCTGGTGCTGGTGCCGTGCCTGAATGATCACCCGGAGTGGGTGAGGGTGTTGGCTGATATGTGTGAAAAGGCCTGAATCGAAGGGGCCGCTTTGCGG‑CCCCTTCCGACCGGTCATACATCCAGCTTCTTGTGCTTc > 7:250103/1‑147 (MQ=255) cAGGAGCTGGTGCTGGTGCCGTGCCTGAATGATCACCCGGAGTGGGTGAGGGTGTTGGCTGATATGTGTGAAAAGGCCTGAATCGAAGGGGCCGCTTTGCGG‑CCCCTTCCGACCGGTCATACATCCAGCTTCTTGTGCTTCCAGCTGt > 2:171211/1‑148 (MQ=255) ggAGCTGGTGCTGGTGCCGTGCCTGAATGATCACCCGGAGTGGGTGAGGGTGTTGGCTGATATGTGTGAAAAGGCCTGAATCGAAGGGGCCGCTTTGAGG‑CCCCTTCCGACCGGTCATACATACAGCTTCTTGTGCTTCCAGCGGTCaa > 2:122875/1‑148 (MQ=255) gctggtgctggtGCCGTGCCTGAATGATCACCCGGAGTGGGTGAGGGTGTTGGCTGATATGTGTGAAAAGGCCTGAATCGAAGGGGCCGCTTTGCGG‑CCCCTTCCGACCGGTCATACATCCAGCTTCTTGTGCTTCCAGCTGTCATTAc < 6:231743/149‑1 (MQ=255) tggtgctggtgcCGTGCCTGAATGATCACCCGGAGTGGGTGAGGGTGTTGGCTGATATGTGTGAAAAGGCCTGAATCGAAGGGGCCGCTTTGCGG‑CCCCTTCCGACCGGTCCTACATCCAGCTTCTTGTGCTTCCAGCTGTCATTAccc > 5:24870/1‑149 (MQ=255) gtgctggtgcCGTGCCTTAATGATCACCCGGAGTGGGTTTGGGTGTTGGCTTATATGTGTGAAAAGGCCTGAATCGAAGGGGCCGCTTTGCGG‑CCCCTTCCGACCGGTCATACATCCAGCTTCTTGTGCTTCCAGCTGTCATTACCCgg < 6:24870/149‑1 (MQ=255) gtgccgtgccTGAATGATCACCCGGAGTGGGTGAGGGTGTTGGCTGATATGTGTGAAAAGGCCTGAATCGAAGGGGCCGCTTTGCGG‑CCCCTTCCGACCGGTCATACATCCAGCTTCTTGTGCTTCCAGCTGTCATTACCCGGCAGAAt > 1:243281/1‑149 (MQ=255) tgccgtgccTGAATGATCACCCGGAGTGGGTGAGGGTGTTGGCTGATATGTGTGAAAAGGCCTGAATCGAAGGGGCCGCGTTGCGG‑CCCCTTGCGACCGGTCATACATCCAGCTTCTTGTGCTTGCCGCTGGCATTACCCGGCAGAAt > 1:254915/1‑148 (MQ=255) ccgtgccTGAATGATCACCCGGAGTGGGTGAGGGTGTTGGCTGATATGTGTGAAAAGGCCTGAATCGAAGGGGCCGCTTTGCGG‑CCCCTTCCGACCGGTCATACATCCAGCTTCTTGTGCTTCCAGCTGTCATTACCCGGCAGAATCAg < 1:171211/149‑1 (MQ=255) ccTGAATGATCACCCGGAGTGGGTGAGGGTGTTGGCTGATATGTGTGAAAAGGCCTGAATCGAAGGGGCCGCTTTGCGG‑CCCCTTCCGACCGGTCATACATCCAGCTTCTTGTGCTTCCAGCTGTCATTACCCGGCAGAATCAGGTTCa < 8:138351/149‑1 (MQ=255) cTGAATGATCACCCGGAGTGGGTGAGGGTGTTGGCTTATATGTGTGAAAAGGCCTGAATCGAAGGGGCCGCTTTGCGG‑CCCCTTCCGACCGGTCATACATCCAGCTTCTTGTGCTTCCAGCTGTCATTACCCGGCAGAATCAGGTTCAg < 3:258900/149‑1 (MQ=255) tGAATGATCACCCGGAGTGGGTGAGGGTGTTGGCTGATATGTGTGAAAAGGCCTGAATCGAGGGGGCCGCTTTGCGG‑CCCCTTCCGACCGGTCATACATCCAGCTTCTTGTGCTTCCAGCTGTCATTACCCGGCAGAATCAGGTTCAgg > 1:42411/1‑149 (MQ=255) tGATCACCCGGAGTGGGTGAGGGTGTTGGCTGATATGTGTGAAAAGGCCTGAATCGAAGGGGCCGCTTTGCGG‑CCCCTTCCGACCGGTCATACATCCAGCTTCTTGTGCTTCCAGCTGTCATTACCCGGCAGAATCAGGTTCAGGGCaa > 1:27344/1‑149 (MQ=255) aCCCGGAGTGGGTGAGGGTGTTGGCTGATATGTGTGAAAAGGCCTGAATCGAAGGGGCCGCTTTGCGG‑CCCCTTCCGACCGGTCATACATCCAGCTTCTTGTGCTTCCAGCTGTc > 5:97573/1‑115 (MQ=255) aCCCGGAGTGGGTGAGGGTGTTGGCTGATATGTGTGAAAAGGCCTGAATCGAAGGGGCCGCTTTGCGG‑CCCCTTCCGACCGGTCATACATCCAGCTTCTTGTGCTTCCAGCTGTc < 6:97573/115‑1 (MQ=255) aCCCGGAGTGGGTGAGGGTGTTGGCTGATATGTGTGAAAAGGCCTGAATCGAAGGGGCCGCTTTGCGG‑CCCCTTCCGACCGGTCATACATCCAGCTTCTTGTGCTTCCAGCTGTCATTACCCGGCAGAATCAGGTTCAGGGCAATAGc > 4:79116/1‑148 (MQ=255) gTGAGGGTGTTGGCTTATATGTGTGAAAAGGCCTGAATCGACGGGGCCGCTTTGCGG‑CCCCTTCCGACCGGTCATACATCCAGCTTCTTGTGCTTCCAGCTGTCATTACCCGGCAGAATCAGGTTCAGGGCAATAGCCACAATGGCGca < 1:122875/149‑1 (MQ=255) aGGGTGTTGGCTGATATGTGTGAAAAGGCCTGAATCGATGGGGCCGCTTTGCGG‑CCCCTTCCGACCGGTCATACATCCAGCTTCTTGTGCTTCCAGCTGTCATTACCCGGCAGAATCAGGTTCAGGGCAATAGCCACAATGGCGcaca < 2:42411/148‑1 (MQ=255) tgtTGGCTGATATGTGTGAAAAGGCCTGAATCGAAGGGGCCGCTTTGCGG‑CCCCTTCCGACCGGTCATACATCCAGCTTCTTGTGCTTCCAGCTGTCATTACCCGGCAGAATCAGGTTCAGGGCAATAGCCACAATGGCGCACAGGGc > 6:9257/1‑148 (MQ=255) tGATATGTGTGAAAAGGCCTGAATCGAAGGGGCCGCTTTGCGG‑CCCCTTCCGACCGGTCATACATCCAGCTTCTTGTGCTTCCAGCTGTCATTACCCGGCAGAATCAGGTTCAGGGCAATa < 2:75548/121‑1 (MQ=255) tGATATGTGTGAAAAGGCCTGAATCGAAGGGGCCGCTTTGCGG‑CCCCTTCCGACCGGTCATACATCCAGCTTCTTGTGCTTCCAGCTGTCATTACCCGGCAGAATCAGGTTCAGGGCAATa > 1:75548/1‑121 (MQ=255) tcttgtgtgAAAAGGCCTGAATCGAAGGGGCCGCTTTGCGG‑CCCCTTCCGACCGGTCATACATCCAGCTTCTTGTGCTTCCAGCTGTCATTACCCGGCAGAATCAGGTTCAGGGCAATAGCCACAATGGCGCACAGGGCGATGCCCtt < 2:48623/145‑1 (MQ=255) tatGTGTGAAAAGGCCTGAATCGAAGGGGCCGCTTTGCGG‑CCCCTTCCGACCGGTCATACATCCAGCTTCTTGTGCTTCCAGCTGTCATTACCCGGCAGAATCAGGTTCAGGGCAATAGCCACAATGGCGCACAGGGCGATGCCCTTCa < 3:79116/149‑1 (MQ=255) tgtgtgAAAAGGCCTGAATCGAAGGGGCCGCTTTGCGG‑CCCCTTCCGACCGGTCATACATCCAGCTTCTTGTGCTTCCAGCTGTCATTACCCGGCAGAATCa > 5:264625/1‑102 (MQ=255) tgtgtgAAAAGGCCTGAATCGAAGGGGCCGCTTTGCGG‑CCCCTTCCGACCGGTCATACATCCAGCTTCTTGTGCTTCCAGCTGTCATTACCCGGCAGAATCa < 6:264625/102‑1 (MQ=255) tgtgtgAAAAGGCCTGAATCGAAGGGGCCGCTTTGCGG‑CCCCTTCCGACCGGTCATACATCCAGCTTCTTGTGCTTCCAGCTGTCATTACCCGGCAGAATCAGGTTCAGGGCAATAGCCACAATGGCGCACAGGGCGATGCCCTTCAgg < 5:9257/149‑1 (MQ=255) gtgtgAAAAGGCCTGAATCGAAGGGGCCGCTTTGCGGACCCCTTCCGACCGGTCATACATCCAGCTTCTTGTGCTTCCAGCTGTCATTACCCGGCAGAATCAGGTTAAGGGCAATAGCCACAATGGCGCACAGGGCGATGCCCtt > 1:48623/1‑145 (MQ=255) gtgtgAAAAGGCCTGAATCGAAGGGGCCGCTTTGCGG‑CCCCTTCCGACCGGTCATACATCCAGCTTCTTGTGCTTCCAGCTGTCATTACCCGGCAGAATCAGGTTCAGGGCAATAGCCACAATGGCGCACAGGGCGATGCCCtt < 6:223076/144‑1 (MQ=255) gtgtgAAAAGGCCTGAATCGAAGGGGCCGCTTTGCGG‑CCCCTTCCGACCGGTCATACATCCAGCTTCTTGTGCTTCCAGCTGTCATTACCCGGCAGAATCAGGTTCAGGGCAATAGCCACAATGGCGCACAGGGCGATGCCCtt > 5:223076/1‑144 (MQ=255) gtgtgAAAAGGCCTGAATCGAAGGGGCCGCTTTGCGG‑CCCCTTCCGACCGGTCATACATCCAGCTTCTTGTGCTTCCAGCTGTCATTACCCGGCAGAATCAGGTTCAGGGCAATAGCCACAATGGCGCACAGGGCGATGCCCTTCAGGc > 5:259072/1‑149 (MQ=255) tgAAAAGGCCTGAATCGAAGGGGCCGCTTTGCGG‑CCCCTTCCGACCGGTCATACATCCAGCTTCTTGTGCTTCCAGCTGTCATTACCCGGCAGAATCAGGTTCAGGGCAATAGCCACAATGGCGCACAGGGCGATGCCCTTCAGGcccc < 7:204710/149‑1 (MQ=255) gAAAAGGCCTGAATCGAAGGGGCCGCTTTGCGG‑CCCCTTCCGACCGGTCATACAACCAGCTTGTTGTGCTTGCAGCTGTCCTTACCCGGCAGAATCAGGTTCAGGGCAATAGCCAGAATGGCGCACAGGGCCATGCCCTTCAGGCCCCa > 3:12501/1‑149 (MQ=255) aaaaGGCCTGAATCGAAGGGGCCGCTTTGCGG‑CCCCTTCCGACCGGTCATACATCCAGCTTCTTGTGCTTCCAGCTGTCATTACCCGGCAGAATCAGGTTCAGGGCAATAGCCACAATGGCGCACAGGGCGATGCCCTTCAGGCCCCa < 6:259072/148‑1 (MQ=255) gAAGGGGCCGCAAAGCGG‑CCCCTTCCGACCGGTCATAAATCCAGCTTCTTGTGCTTCCAGCTGTCATTACCCGGCAGAATCAGGTTCAGGGCAATAGCCACAATGGCGCACAGGGCGATGCCCTTCAGGCCCCAGTCATCCGGGCCGt > 8:82436/1‑148 (MQ=255) aGGGGCCGCTTTGCGG‑CCCCTTCCGACCGGTCATACATCCAGCTTCTTGTGCTTCCAGCTGTCATTACCCGGCAGAATCAGGTTCAGGGCAATAGCCACAATGGCGCACAGGGCGATGCCCTTCAGGCCCCAGTCATCCGGGCCGTCAc < 8:250103/149‑1 (MQ=255) gCCGCTTTGCGG‑CCCCTTCCGACCGGTCATACATCCAGCTTCTTGTGCTTCCAGCTGTCATTACCCGGCAGAATCAGGTTCAGGGCAATAGCCACAATGGCGCACAGGGCGATGCCCTTCAGGCCCCAGTCATCCGGGCCGTCACCGCt < 1:58958/149‑1 (MQ=255) ccGCTTTGCGG‑CCCCTTCCGACCGGTCATACATCCAGCTTCTTGTGCTTCCAGCTGTCATTACCCGGCAGAATCAGGTTCAGGGCAATAGCCACAATGGCGCACAGGGCGATGCCCTTCAGGCCCCAGTCATCCGGGCCGTCACCGCTg < 2:243281/149‑1 (MQ=255) ccGCTTTGCGG‑CCCCTTCCGACCGGTCATACATCCAGCTTCTTGTGCTTCCAGCTGTCATTACCCGGCAGAATCAGGTTCAGGGCAATAGCCACAATGGCGCACAGGGCGATGCCCTTCAGGCCCCAGTCATCCGGGCCGTCACCGCTg < 2:254915/149‑1 (MQ=255) ccGCTTTGCGG‑CCCCTTCCGACCGGTCATACATCCAGCTTCTTGTGCTTCCAGCTGTCATTACCCGGCAGAATCAGGTTCAGGGCAATAGCCACAATGGCGCACAGGGCGATGCCCTTCAGGCCCCAGTCATCCGGGCCGTCACCGCTg > 6:169353/1‑149 (MQ=255) cGCTTTGCGG‑CCCCTTCCGACCGGTCATACATCCAGCTTCTTGTGCTTCCAGCTGTCATTACCCGGCAGAATCAGGTTCAGGGCAATAGCCACAATGGCGCACAGGGCGATGCCCTTCAGGCCCCAGTCATCCGGGCCGTCACCGCTGc > 4:127640/1‑149 (MQ=255) | GAAGAGATAGGCATGCGTGGCAGCGAGCAGTTTGTCGAGGCGGGCGGCCAGGAGCTGGTGCTGGTGCCGTGCCTGAATGATCACCCGGAGTGGGTGAGGGTGTTGGCTGATATGTGTGAAAAGGCCTGAATCGAAGGGGCCGCTTTGCGG‑CCCCTTCCGACCGGTCATACATCCAGCTTCTTGTGCTTCCAGCTGTCATTACCCGGCAGAATCAGGTTCAGGGCAATAGCCACAATGGCGCACAGGGCGATGCCCTTCAGGCCCCAGTCATCCGGGCCGTCACCGCTGC > NC_002947/863755‑864043 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |