Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 127,122 C→G 12.5% intergenic (‑32/+38) thrB ← / ← PP_0122 homoserine kinase/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947127,1220CG12.5% 121.3 / 9.5 48intergenic (‑32/+38)thrB/PP_0122homoserine kinase/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (21/21);  new base G (3/3);  total (24/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.62e-01

GAAATTGCTGTTCTCGGTACCGGCGGCAATGCCCTGGAAGTCGAGCAGACGGCCCAGCTCGTACGGCGCCAGAAAGGTTTCCAGCTCAGGCCGGGTCACGGGGGTGAAGACTGACATGATGAAAAATTGCCCATACGGGCACTTCCGCCGGGAAGTGCCGGGTTGATGTGAACGGTGCGCGTCAGCTTACCACTCGAAGATCTTCCAGGACGGAATCAGCATGTCCGGCTGGTCGGATCGAATGAAATTGGCATCAGTGCCATCAGCGCGTACCAGAAAATAAGGCT  >  NC_002947/126974‑127260
                                                                                                                                                    |                                                                                                                                          
gaaATTGCTGTTCTCGGTACCGGCGGCAATGCCCTGGAAGTCGAGCAGACGGCCCAGCTCGTACGGCGCCAGAAAGGTTTCCAGCTCAGGCCGGGTCACGGGGGTGAAGACTGACATGATGAAAAATTGCCCATACGGGCACTTccgcc                                                                                                                                            >  8:204576/1‑149 (MQ=255)
 aaaTTGCTGTTCTCGGTACCGGCGGCAATGCCCTGGAAGTCGAGCAGACGGCCCAGCTCGTACGGCGCCAGAAAGGTTTCCAGCTCAGGCCGGGTCACGGGGGTGAAGACTGACATGATGAAAAATTGCCCATACGGGCACTTccgccg                                                                                                                                           >  7:210562/1‑149 (MQ=255)
      gCTGTTCTCGGTACCGGCGGCAATGCCCTGGAAGTCGAGCAGACGGCCCAGCTCGTACGGCGCCAGAAAGGTTTCCAGCTCAGGCCGGGTCACGGGGGTGAAGACTGACATGATGAAAAATTGCCCATACGGGCACTTCCGCCGGGAAg                                                                                                                                      >  2:80131/1‑149 (MQ=255)
          ttCTCGGTACCGGCGGCAATGCCCTGGAAGTCGAGCAGACGGCCCAGCTCGTACGGCGCCAGAAAGGTTTCCAGCTCAGGCCGGGTCACGGGGGTGAAGACTGACATGATGAAAAATTGCCCATACGGGCACTTCCCGGCGGAAGTGcc                                                                                                                                  <  2:100732/149‑1 (MQ=255)
          ttCTCGGTACCGGCGGCAATGCCCTGGAAGTCGAGCAGACGGCCCAGCTCGTACGGCGCCAGAAAGGTTTCCAGCTCAGGCCGGGTCACGGGGGAGAAGACTGACATGATGAAAAATTGCCCATACGGGCACTTCCGCCGGGAAGTGcc                                                                                                                                  >  7:239810/1‑149 (MQ=255)
            ctcGGTACCGGCGGCAATGCCCTGGAAGTCGAGCAGACGGCCCAGCTCGTACGGCGCCAGAAAGGTTTCCAGCTCAGGCCGGGTCACGGGGGTGAAGACTGACATGATGAAAAATTGCCCATACGGGCACTTCCGCCGGGAAGTGCCgg                                                                                                                                >  3:241500/1‑149 (MQ=255)
             tcGGTACCGGCGGCAATGCCCTTGAAGTCGAGCAGACGGCCCAGCTCGTACGGCGCCAGAAAGGTTTCCAGCTCAGGCCGGGTCACTGGGGTGAAGACTGACATGATGAAAAATTGCCCATACGGGCACTTCCGCCGGGAAGTGCCgg                                                                                                                                <  8:210562/148‑1 (MQ=255)
               ggTACCGGCGGCAATGCCCTGGAAGTCGAGCAGACGGCCCAGCTCGTACGGCGCCAGAAAGGTTTCCAGCTCAGGCCGGGTCACGGGGGTGAAGACTGACATGATGAAAAATTGCCCATACGGGCACTTCCGCCGGGAAGTGCCGGGtt                                                                                                                             <  4:241500/149‑1 (MQ=255)
               gctACCGGCGGCAATGCCCTGGAAGTCGAGCAGACGGCCCAGCTCGTACGGCGCCAGAAAGGTTTCCAGCTCAGGCCGGGTCACGGGGGTGATGACTGACAAGATGAAAAATTGCCCATACCGCCACTTCCCGGCGGAAGTGcccgtat                                                                                                                             <  1:207923/147‑6 (MQ=255)
                     ggcggcAATGCCCTGGAAGTCGAGCAGACGGCCCAGCTCGTACGGCGCCAGAAAGGTTTCCAGCTCAGGCCGGGTCACGGGGGTGAAGACTGACATGATGAAAAATTGCCCATACGGGCACTTCCGCCGGGAAGTGCCGGGTTGAtgtg                                                                                                                       <  1:131563/149‑1 (MQ=255)
                              gCCCTGGAAGTCGAGCAGACGGCCCAGCTCGTACGGCGCCAGAAAGGTTTCCAGCTCAGGCCGGGTCACGGGGGTGAAGACTGACATGATGAAAAATTGCCCATACGGGCACTTCTGCCGGGAAGTGCCGGGTTGATGTGAACGGTgcg                                                                                                              >  5:155974/1‑149 (MQ=255)
                                   ggAAGTCGAGCAGACGGCCCAGCTCGTACGGCGCCAGAAAGGTTTCCAGCTCAGGCCGGGTCACGGGGGTGAAGACTGACATGATGAAAAATTGCCCATACGGGCACTTCCGCCGGGAAGTGCCGGGTTGAt                                                                                                                          >  6:70854/1‑132 (MQ=255)
                                   ggAAGTCGAGCAGACGGCCCAGCTCGTACGGCGCCAGAAAGGTTTCCAGCTCAGGCCGGGTCACGGGGGTGAAGACTGACATGATGAAAAATTGCCCATACGGGCACTTCCGCCGGGAAGTGCCGGGTTGAt                                                                                                                          <  5:70854/132‑1 (MQ=255)
                                    gAAGTCGAGCAGACGGCCCAGCTCGTACGGCGCCAGAAAGGTTTCCAGCTCAGGCCGGGTCACGGGGGTGAAGACTGACATGATGAAAAATTGCCCATACGGGCACTTCCGCCGGGAAGTGCCGGGTTGATGTGAACGGTGCGCGt                                                                                                           >  3:24633/1‑146 (MQ=255)
                                    gAAGTCGAGCAGACGGCCCAGCTCGTACGGCGCCAGAAAGGTTTCCAGCTCAGGCCGGGTCACGGGGGTGAAGACTGACATGATGAAAAATTGCCCATACGGGCACTTCCGCCGGGAAGTGCCGGGTTGATGTGAACGGTGCGCGTCa                                                                                                         <  4:24633/148‑1 (MQ=255)
                                    gAAGTCGAGCAGACGGCCCAGCTCGTACGGCGCCAGAAAGGTTTCCAGCTCAGGCCGGGTCACGGGGGTGAAGACTGACATGATGAAAAATTGCCCATACGGGCACTTCCGCCGGGAAGTGCCGGGTTGATGTGAACGGTGCGCGTCAg                                                                                                        >  5:27415/1‑149 (MQ=255)
                                     aaGTCGAGCAGACGGCCCAGCTCGTAGGGCGCCAGAAAGGTTTCCAGCTCAGGCCGGGTCACGGGGGTGAAGACTGACATGATGAAAAATTGCCCATACGGGCACTTCCGCCGGGAAGTGCCGGGTTGATGTGAACGGTGCGCGTCAGc                                                                                                       <  7:199463/149‑1 (MQ=255)
                                      aGTCGAGCAGACGGCCCAGCTCGTACGGCGCCAGAAAGGTTTCCAGCTCAGGCCGGGTCACGGGGGTGAAGACTGACATGATGAAAAATTGCCCATACGGGCACTTCCGCCGGGAAGTGCCGGGTTGATGTGAACGGTGCGCGTCGGCt                                                                                                      <  1:80131/149‑1 (MQ=255)
                                        tCGAGCAGACGGCCCAGCTCGTACGGCGCCAGAAAGGTTTCCAGCTCAGGCCGGGTCACGGGGGTGAAGACTGACATGATGAAAAATTGCCCATACGGGCACTTCCGCCGGGAAGTGCCGGGTTGATGTGAACGGTGCGCGTCAGCTTa                                                                                                    <  3:118102/149‑1 (MQ=255)
                                          gAGCAGACGGCCCAGCTCGTACGGCGCCAGAAAGGTTTCCAGCTCAGGCCGGGTCACGGGGGTGAAGACTGACATGATGAAAAATTGCCCATACGGGCACTTCCGCCGGGAAGTGCCGGGTTGATGTGAACGGTGCGCGTCAGCTTAc                                                                                                   <  6:176933/148‑1 (MQ=255)
                                               gACGGCCCAGCTCGTACGGCGCCAGAAAGGTTTCCAGCTCAGGCCGGGTCACGGGGGTGAAGACTGACATGATGAAAAATTGCCCATACGGGCACTTCCGCCGGGAAGTGCCGGGTTGATGTGAACGGTGCGCGTCAGCTTACCACTc                                                                                              >  8:244961/1‑148 (MQ=255)
                                                aCGGCCCAGCTCGTACGGCGCCAGAAAGGTTTCCAGCTCAGGCCGGGTCACGGGGGTGAAGACTGACATGATGAAAAATTGCCCATACGGGCACTTCCGCCGGGAAGTGCCGGGTTGATGTGAACGGTGCGCGTCAGCTTACCACTCg                                                                                             >  7:117380/1‑148 (MQ=255)
                                                        gCTCGTACGGCGCCAGAAAGGTTTCCAGCTCAGGCCGGGTCACGGGGGTGAAGACTGACATGATGAAAAATTGCCCATACGGGCACTTCCGCCGGGAAGTGCCGGGTTGATGTGAACGGTGCGCGTCAGCTTACCACTCGAAGATCTTc                                                                                    <  5:253112/149‑1 (MQ=255)
                                                        gCTCGTACGGCGCCAGAAAGGTTTCCAGCTCAGGCCGGGTCACGGGGGTGAAGACTGACATGATGAAAAATTGCCCATACGGGCACTTCCGCCGGGAAGTGCCGGGTTGATGTGAACGGTGCGCGTCAGCTTACCACTCGAAGATCTTc                                                                                    <  7:204576/149‑1 (MQ=255)
                                                           cGTACGGCGCCAGAAAGGTTTCCAGCTCAGGCCGGGTCACGGGGGTGAAGACTGACATGATGAAAAATTGCCCATACGGGCACTTCCGCCGGGAAGTGCCGGGTTGATGTGAACGGTGCGCGTCAGCTTACCACTCGAAGATCTTCCAg                                                                                 >  7:137089/1‑149 (MQ=255)
                                                            gTACGGCGCCAGAAAGGTTTCCAGCTCAGGCCGGGTCACGGGGGTGAAGACTGACATGATGAAAAATTGCCCATACGGGCACTTCCGCCGGGAAGTGCCGGGTTGATGTGAACGGTGCGCGTCAGCTTACCACTCGAAGATCTTCCa                                                                                  <  8:233181/147‑1 (MQ=255)
                                                            gTACGGCGCCAGAAAGGTTTCAAGCTCAGGCCGGGTCACGGGGGTGAAGACTGACATGATGAAAAATTGCCAATACGGGCACTTCCGCCGGGAAGTGCCGGGTTGATGTGAACGGTTCGCTTCAGCTTACAAATCGAAGATCTTCCAg                                                                                 >  8:190155/1‑148 (MQ=255)
                                                               cGGCGCCAGAAAGGTTTCCAGCTCAGGCCGGGTCACGGGGGTGAAGACTGACATGATGAAAAATTGCCCATACGGGCACTTCAGCCGGGAAGTGCCGGGTTGATGTGAACGGTGCGCGTCAGCTTACCACTCGAAGATCTTCCAGGACg                                                                             <  6:155974/149‑1 (MQ=255)
                                                                 gcgcCAGAAAGGTTTCCAGCTCAGGCCGGGTCACGGGGGTGAAGACTGACATGATGAAAAATTGCCCATACGGGCACTTCCGCCGGGAAGTGCCGGGTTGATGTGAACGGTGCGCGTCAGCTTACCACTCGAAGATCTTCCAGGACGGa                                                                           <  8:137089/149‑1 (MQ=255)
                                                                         aaGGTTTCCAGCTCAGGCCGGGTCACGGGGGTGAAGACTGACATGATGAAAAATTGCCCATACGGGCACTTCCGCCGGGAAGTGCCGGGTTGATGTGAACGGTGCGCGTCAGCTTACCACTCGAAGATCTTCCAGGACGGAATCAGCa                                                                    <  6:27415/148‑1 (MQ=255)
                                                                                 cAGCTCAGGCCGGGTCACGGGGGTGAAGACTGACATGATGAAAAATTGCCCATACGGGCACTTCCGCCGGGAAGTGCCGGGTTGATGTGAACGGTGCGCGTCAGCTTACCACTCGAAGATCTTCCAGGACGGAATCAGCATGTCCGGCt                                                           >  5:272336/1‑149 (MQ=255)
                                                                                         gCCGGGTCACGGGGGTGAAGACTGACATGATGAAAAATTGCCCATACGGGCACTTCCGCCGGGAAGTGCCGGGTTGATGTGAACGGTGCGCGTCAGCTTACCACTCGAAGATCTTCCAGGAGGGAATCAGCATGTCAGGCTGGTCGGAt                                                   >  7:91107/1‑149 (MQ=255)
                                                                                                         gAAGACTGACATGATGAAAAATTGCCCATACGGGCACTTCCGCCGGGAAGTGCCGGGTTGATGTGAACGGTGCGCGTCAGCTTACCACTCGAAGATCTTCCAGGACGGAATCAGCATGTCCGGCTGGTCGGATCGAATGAAATTGGCAt                                   >  5:143344/1‑149 (MQ=255)
                                                                                                              cTGACATGATGAAAAATTGCCCATACGGGCACTTCCGCCGGGAAGTGCCGGGTTGATGTGAACGGTGCGCGTCAGCTTACCACTCGAAGATCTTCCAGGACGGAATCAGCATGTCCGGCTGGTCGGATCGAATGAAATTGGCATCAGTg                              <  8:117380/149‑1 (MQ=255)
                                                                                                                      atgaAAAATTGCCCATACGGGCACTTCCGCCGGGAAGTGCCGGGTTGATGTGAACGGTGCGCGTCAGCTTACCACTCGAAGATCTTCCAGGACGGAATCAGCATGTCCGGCTGGTCGGATCGAATGAAATTGGCATCAGTGCCATCAg                       <  7:244961/148‑1 (MQ=255)
                                                                                                                       tgaAAAATTGCCCATACGGGCACTTACGCCGGGAAGTGCCGGGTTGATGTGAACGGTGCGCGTCAGCTTACCACTCGAAGATCTTCCAGGACGGAATCAGCATGTCCGGCTGGTCGGATCGAATGAAATTGGCATCAGTGCCATCAgc                      <  7:190155/148‑1 (MQ=255)
                                                                                                                        gaAAAATTGCCCATACGGGCACTTCCGCCGGGAAGTGCCGGGTTGATGTGAACGGTGCGCGTCAGCTTACCACTCGAAGATCTTCCAGGACGGAATCAGCATGTCCGGCTGGTCGGATCGAATGAAATTGGCATCAGTGCCATCAgcgc                    >  8:234493/1‑149 (MQ=255)
                                                                                                                                 cccATACGGGCACTTCCGCCGGGAAGTGCCGGGTTGATGTGAACGGTGCGCGTCAGCTTACCACTCGAAGATCTTCCAGGACGGAATCAGCATGTCCGGCTGGTCGGATCGAATGAAAtt                                        >  4:164254/1‑120 (MQ=255)
                                                                                                                                 cccATACGGGCACTTCCGCCGGGAAGTGCCGGGTTGATGTGAACGGTGCGCGTCAGCTTACCACTCGAAGATCTTCCAGGACGGAATCAGCATGTCCGGCTGGTCGGATCGAATGAAAtt                                        <  3:164254/120‑1 (MQ=255)
                                                                                                                                  ccATACGGGCACTTCCGCCGGGAAGTGCCGGGTTGATGTGAACGGTGCGCGTCAGCTTACCACTCGAAGATCTTCCAGGACGGAATCAGCATGTACGGCTGGTCGGATCGAATGAAATTGGCATCACTGCAAt                          >  8:190068/1‑133 (MQ=255)
                                                                                                                                  ccATACGGGCACTTCCGCCAGGAAGTGCCGGGTTGATGTGAACGGTGCGCGTCAGCTTACCACTCGAAGATCTTCCAGGACGGAATCAGCATGTCCGGCTGGTCGGATCGAATGAAATTGGCATCAGTGCCATCAGCGCGTACCAGaa           >  3:258731/1‑148 (MQ=255)
                                                                                                                                    aTACGGGCACTTCCGCCGGGAAGTGCCGGGTTGATGTGAACGGTGCGCGTCAGCTTACCACTCGAAGATCTTCCAGGACGGAATCAGCATGTCCGGCTGGTCGGATCGAAt                                              <  6:62071/111‑1 (MQ=255)
                                                                                                                                    aTACGGGCACTTCCGCCGGGAAGTGCCGGGTTGATGTGAACGGTGCGCGTCAGCTTACCACTCGAAGATCTTCCAGGACGGAATCAGCATGTCCGGCTGGTCGGATCGAAt                                              >  5:62071/1‑111 (MQ=255)
                                                                                                                                    aTACGGGCACTTCCGCCGGGAAGTGCCGGGTTGATGTGAACGGTGCGCGTCAGCTTACCACTCGAAGATCTTCCAGGACGGAATCAGCATGTCCGGCTGGTCGGATCGAATGAAATTGGCATCAGTGCCATCAGCGCGTACCAGaaaa         >  1:76296/1‑148 (MQ=255)
                                                                                                                                       ccggCACTTCCCGGCGGAAGTGCCGGGTTGATGTGAACGGTGCGCGTCAGCTTACCACTCGAAGATCTTCCAGGACGGAATCAGCATGTCCGGCTGGTCGGATCGAATGAAATTGGCATCAGTGCCATCAGCGCGTACCAGAAAATAAg     >  7:57542/3‑149 (MQ=255)
                                                                                                                                       cGGGCACTTCCGCCGGGAAGTGCCGGGTTGATGTGAACGGTGCGCGTCAGCTTACCACTCGAAGATCTTCCAGGACGGAATCAGCATGTCCGGCTGGTCGGATCGAATGAAATTGGCATCAGTGCCATCAGCGCGTACCAGAAAATaa      <  8:239810/148‑1 (MQ=255)
                                                                                                                                        cggCACTTCCCGGCGGAAGTGCCGGGTTGATGTGAACGGTGCGCGTCAGCTTACCACTCGAAGATCTTCCAGGACGGAATCAGCATGTCCGGCTGGTCGGATCGAATGAAATTGGCATCAGTGCCATCAGCGCGTACCAGaaa          >  8:251813/2‑143 (MQ=255)
                                                                                                                                        cggCACTTCCCGGCGGAAGTGCCGGGTTGATGTGAACGGTGCGCGTCAGCTTACCACTCGAAGATCTTCCAGGACGGAATCAGCATGTCCGGCTGGTCGGATCGAATGAAATTGGCATCAGTGCCATCAGCGCGTACCAGaaa          <  7:251813/142‑1 (MQ=255)
                                                                                                                                        cggCACTTCCCGGCGGAAGTGCCGGGTTGATGTGAACGGTGCGCGTCAGCTTACCACTCGAAGATCTTCCAGGACGGAATCAGCATGTCCGGCTGGTCGGATCGAATGAAATTGGCATCAGTGCCATCAGCGCGTACCAGAAAATAAgg    >  6:53780/2‑149 (MQ=255)
                                                                                                                                          gCACTTCCGCCGGGAAGTGCCGGGTTGATGTGAACGGTGCGCGTCAGCTTACCACTCGAAGATCTTCCAGGACGGAATCAGCATGTCCGGCTGGTCGGATCGAATGAAATTGGCATCAGTGCCATCAGCGCGTACCAGAAAATAAGGCt  >  5:36830/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                          
GAAATTGCTGTTCTCGGTACCGGCGGCAATGCCCTGGAAGTCGAGCAGACGGCCCAGCTCGTACGGCGCCAGAAAGGTTTCCAGCTCAGGCCGGGTCACGGGGGTGAAGACTGACATGATGAAAAATTGCCCATACGGGCACTTCCGCCGGGAAGTGCCGGGTTGATGTGAACGGTGCGCGTCAGCTTACCACTCGAAGATCTTCCAGGACGGAATCAGCATGTCCGGCTGGTCGGATCGAATGAAATTGGCATCAGTGCCATCAGCGCGTACCAGAAAATAAGGCT  >  NC_002947/126974‑127260

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑