| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 900,536 | T→G | 10.5% | intergenic (+28/+17) | PP_0784 → / ← cysZ | hypothetical protein/sulfate transport protein CysZ |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 900,536 | 0 | T | G | 10.5% | 154.2 / 10.4 | 57 | intergenic (+28/+17) | PP_0784/cysZ | hypothetical protein/sulfate transport protein CysZ |
| Reads supporting (aligned to +/- strand): ref base T (27/24); new base G (4/2); total (31/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.78e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.17e-01 | |||||||||||
GGCGTTCGGCGGGTCAAGCGACCTTGCTAACCTCGAGAAGGTTAAGGCAGTGGAACATCTAATACTGCTTTCTCAGATTGCCACCCTCGAACCCTACAGTTTCTCCGACTTCTAAAAAGCAATCCGCAAAAAGGGGCTGCCCTGCAGCCCCTCGCTCCTTCAGCTCCGCTCCCGCACCCAGAACAACGTAGCCCCGGCCACTGCCGCCGGCATCATCAGCACGTTCACGCCTGGAATCATCAGCGCCAGGTAGGTGATGCCGCCAAAGCCCAGGCTCTGCCAGCGCTTCTG > NC_002947/900394‑900684 | ggCGTTCGGCGGGTCAAGCGACCTTGCTAACCTCGAGAAGGTTAAGGCAGTGGAACATCTAATACTGCTTTCTCAGATTGCCACCCTCGAACCCTACAGTTTCTCCGACTTCTAAAAAGCAATCCGCAAAAAGGGGCTGCCCTGCAGc < 7:229433/148‑1 (MQ=255) gTTCGGCGGGTCAAGCGACCTTGCTAACCTCGAGAAGGTTAAGGCAGTGGAACATCTAATACTGCTTTCTCAGATTGCCACCCTCGAACCCTACAGTTTCTCCGACTTCTAAAAAGCAATCCGCAAAAAGGGGCTGCAGGGCAGcccc < 8:71592/148‑1 (MQ=255) gTTCGGCGGGTCAAGCGACCTTGCTAACCTCGAGAAGGTTAAGGCAGTGGAACATCTAATACTGCTTTCTCAGATTGCCACCCTCGAACCCTACAGTTTCTCCGACTTCTAAAAAGCAATCCGCAAAAAGGGGCTGCAGGGCAGcccc > 7:71592/1‑148 (MQ=255) ttCGGCGGGTCAAGCGACCTTGCTAACCTCGAGAAGGTTAAGGCAGTGGAACATCTAATACTGCTTTCTCAGATTGCCACCCTCGAACACTACAGTTTCTCCGACTTCTAAAAAGCAATCCGCAAAAAGGGGCTGCAGGGCAGCCCCtt > 4:23093/1‑148 (MQ=255) ggcggGTCAAGCGACCTTGCTAACCTCGAGAAGGTTAAGGCAGTGGAACATCTAATACTGCTTTCTCAGATTGCCACCCTCGAACCCTACAGTTTCTCCGACTTCTAAAAAGCAATCCGCAAAAAGGGGCTGCAGGGCAGCCCCtttt < 3:23093/148‑4 (MQ=255) gcggGTCAAGCGACCTTGCTAACCTCGAGAAGGTTAAGGCAGTGGAACATCTAATACTGCTTTCTCAGATTGCCACCCTCGAACCCTACAGTTTCTCCGACTTCTAAAAAGCAATCCGCAAAAAGGGGCTGCCCTGCAGACCCTCGCTc > 7:59483/1‑149 (MQ=255) ctatctCAAGCGACCTTGCTAACCTCGAGAAGGTTAAGGCATTGGAACATCTAATACTGCTTTCTCAGATTGCCACCCTCGAACCCTACAGTTTCTCCGACTTCTAAAAAGCAATCCGCAAAAAGGGGCTGCCCTGCAGCCCCTCGCt < 5:222232/143‑1 (MQ=255) gTCAAGCTAACTTGCTAACCTAGAGAAGGTTAAGGCAGTGGAAAATCTAATACTGCTTTCTCAGCTTGCCACCCTCGACCCCTACAGTTTCTCCGACTTCTAAAAAGCAATCCTCAAAAAGGGGCTGCCCTGCAGCCCCTCGCTCCTTc < 2:172985/149‑1 (MQ=255) aaGCGACCTTGCTAACCTCGAGAAGGTTAAGGCAGTGGAACATCTAATACTGCTTTCTCAGATTGCCACCCTCGAACCCTACAGTTTCTCCGACTTCTAAAAAGCAATCCGCAAAAAGGGGCTGCCCTGCAGCCCCTCGcc > 6:222232/1‑140 (MQ=255) cGACCTGGCTAACCTCGTGACGTTTATGGCTGTTTAAAATCTAATACTGCTTTCTCATATTGCCCCCCCCCACCCCTACTGTTTCTCCGACTTTTAAAACGCAAGCCGCAAAACGGGGCTGCCCTGCAGCCCCTCGCTCCTTCAgctcc < 4:148547/149‑1 (MQ=255) gCTAACCTCGAGAAGGTTAAGGCAGTGGAACATCTAATACTGCTTTCTCAGATTGCCACCCTCGAACCCTACAGTTTCTCCGACTTCTAAAAAGCAATCCGCAAAAAGGGGCTGCCCTGCAGCCCCTCGCTCCTTCAGCTCCGCTCCCg > 5:264665/1‑149 (MQ=255) aCCTCGAGAAGGTTAAGGCAGTGGAACATCTAATACTGCTTTCTCAGATTGCCACCCTCGAACCCTACAGTTTCTCCGACTTCTAAAAAGCAATCCGCAAAAAGGGGCTGCCCTGCAGCCCCTCGCTCCTTCAGCTCCGCTCCCGCAcc < 6:264665/149‑1 (MQ=255) aCCTCGAGAAGGTTAAGGCAGTGGAACATCTAATACTGCTTTCTCAGATTGCCACCCTCGAACCCTACAGTTTCTCCGACTTCTAAAAAGCAATCCGCAAAAAGGGGCTGCCCTGCAGCCCCTCGCTCCTTCAGCTCCGCTCCCGCAcc > 4:174243/1‑149 (MQ=255) cTCGAGAAGGTTAAGGCAGTGGAACATCTAATACTGCTTTCTCAGATTGCCACCCTCGAACCCTACAGTTTCTCCGACTTCTAAAAAGCAATCCGCAAAAAGGGGCTGCCCTGCAGCCCCTc > 3:55505/1‑122 (MQ=255) cTCGAGAAGGTTAAGGCAGTGGAACATCTAATACTGCTTTCTCAGATTGCCACCCTCGAACCCTACAGTTTCTCCGACTTCTAAAAAGCAATCCGCAAAAAGGGGCTGCCCTGCAGCCCCTc < 4:55505/122‑1 (MQ=255) cTCGAGAAGGTTAAGGCAGTGGAACATCTAATACTGCTTTCTCAGATTGCCACCCTCGAACCCTACAGTTTCTCCGACTTCTAAAAAGCAATCCGCAAAAAGGGGCTGCCCTGCAGCCCCTCGCTCCTTCAGCTCCGCTCCCGCACCCa > 6:216406/1‑149 (MQ=255) aGGTTAAGGCAGTGGAACATCTAATACTGCTTTCTCAGATTGCCACCCTCGAACCCTACAGTTTCTCCGACTTCTAAAAAGCAATCCGCAAAAAGGGGCTGCCCTGCAGCCCCTCGCTCCTTCAGCTCCGCTCCCGCACCCAGaacaac < 7:48256/149‑1 (MQ=255) ggTTAAGGCAGTGGAACATCTAATACTGCTTTCTCAGATTGCCACCCTCGAACCCTACAGTTTCTCCGACTTCTAAAAAGCAATCCGCAAAAAGGGGCTGCCCTGCAGCCCCTCGCTCCTTCAGCTCCGCTCCCGCACCCAGAACAACg < 3:174243/149‑1 (MQ=255) ttAAGGCAGTGGAACATCTAATACTGCTTTCTCAGATTGCCACCCTCGAACCCTACAGTTTCTCCGACTTCTAAAAAGCAATCCGCAAAAAGGGGCTGCCCTGCAGCCCCTCGCTCCTTCAGCTCCGCTCCCGCACCCAGAACAACGTa < 4:67203/149‑1 (MQ=255) aaGGCAGTGGAACATCTAATACTGCTTTCTCAGATTGCCACCCTCGAACCCTACAGTTTCTCCGACTTCTAAAAAGCAATCCGCAAAAAGGGGCTGCCCTGCAGCCCCTCGCTCCTTCAgctccgctc < 5:142632/128‑1 (MQ=255) aaGGCAGTGGAACATCTAATACTGCTTTCTCAGATTGCCACCCTCGAACCCTACAGTTTCTCCGACTTCTAAAAAGCAATCCGCAAAAAGGGGCTGCCCTGCAGCCCCTCGCTCCTTCAgctccgctc > 6:142632/1‑128 (MQ=255) aaGGCAGTGGAACATCTAATACTGCTTTCTCAGATTGCCACCCTCGAACCCTACAGTTTCTCCGACTTCTAAAAAGCAATCCGCAAAAAGGGGCTGCCCTGCAGCCCCTCGCTCCTTCAGCTCCGCTCCCGCACCCAGAACAACGTAg < 4:64169/148‑1 (MQ=255) gtaGTGGAACATCTAATACTGCTTTCTCAGATTGCCACCCTCGAACCCTACAGTTTCTCCGACTTCTAAAAAGCAATCCGCAAAAAGGGGCTGCCCTGCAGCCCCTCGCTCCTTCAGCTCCGCTCCCGCACCCAGAACAACGTAGcccc > 6:169971/3‑149 (MQ=255) cAGTGGAACATCTAATACTGCTTTCTCAGATTGCCACCCTCGAACCCTACAGTTTCTCCGACTTCTAAAAAGCAATCCTCAAAAAGGGGCTGCCCTGCAGCCCCTCGCTCCTTCAGCTCCGCTCCCGCACCCAGAACAACGTAGCCCCg < 5:192899/149‑1 (MQ=255) tGGAACATCTAATACTGCTTTCTCAGATTGCCACCCTCGAACCCTACAGTTTCTCCGACTTCTAAAAAGCAATCCGCAAAAAGGGGCTGCCCTGCAGCCCCTCGCTCCTTCAGCTCCGCTCCCGCACCCAGAACAACGTAGCCCCGGc > 1:29179/1‑148 (MQ=255) gAACATCTAATACTGCTTTCTCAGATTGCCACCCTCGAACCCTACAGTTTCTCCGACTTCTAAAAAGCAATCCGCAAAAAGGGGCTGCCCTGCAGCCCCTCGCTCCTTCAGCTCCGCTCCCGCACCCAGAACAACGTAGCCCCGGCCAc > 5:234148/1‑149 (MQ=255) gAACATCTAATACTGCTTTCTCAGATTGCCACCCTCGAACCCTACAGTTTCTCCGACTTCTAAAAAGCAATCCGCAAAAAGGGGCTGCCCTGCAGCCCCTCGCTCCTTCAGCTCCGCTCCCGCACCCAGAACAACGTAGCCCCGGCCAc > 6:109192/1‑149 (MQ=255) tCTAATACTGCTTTCTCAGATTGCCACCCTCGAACCCTACAGTTTCTCCGACTTCTAAAAAGCAATCCGCAAAAAGGGGCTGCCCTGCAGCCCCTCGCTCCTTCAGCTCCGCTCCCGCACCCAGAACAACGTAGCCCCGGCCACTgccg > 5:12127/1‑149 (MQ=255) aaTACTGCTTTCTCAGATTGCCACCCTCGAACCCTACAGTTTCTCCGACTTCTAAAAAGCAATCCGCAAAAAGGGGCTGCCCTGCAGCCCCTCGCTCCTTCAGCTCGGCTCCCGCACCCAGAACAACGTAGCCCCGGCCACTGccgccg > 7:16155/1‑149 (MQ=255) tGCTTTCTCAGATTGCCACCCTCGAACCCTACAGTTTCTCCGACTTCTAAAAAGCAATCCGCAAAAAGGGGCTGCCCTGCAGCCCCTCGCTCCTTCAGCTCCGCTCCCGCACCCAGAACAACGTAGCCCCGGCCACTGCCGCCGGcat > 8:214518/1‑148 (MQ=255) tctcAGATTGCCACCCTCGAACCCTACAGTTTCTCCGACTTCTAAAAAGCAATCCGCAAAAAGGGGCTGCCCTGCAGCCCCTCGCTCCTTCAGCTCCGCTCCCGCACCCAGAACAACGTAGCCCCGGCCACTGCCGCCGGCAtcatca < 5:216406/148‑1 (MQ=255) tcAGATTGCCACCCTCGAACCCTACAGTTTCTCCGACTTCTAAAAAGCAATCCGCAAAAAGGGGCTGCCCTGCAGCCCCTCGCTCCTTCAGCTCCGCTCCCGCACCCAGAACAACGTAGCCCCGGCCACTGCCGCCGGCATCATCAGCa > 6:155357/1‑149 (MQ=255) aGATTGCCACCCTCGAACCCTACAGTTTCTCCGACTTCTAAAAAGCAATCCGCAAAAAGGGGCTGCCCTGCAGCCCCTCGCTCCTTCAGCTCCGCTCCCGCACCCAGAACAACGTAGCCCCGGCCACTGCCGCCGGCATCATCAGCa < 7:32342/147‑1 (MQ=255) cACCCTCGAACCCTACAGTTTCTCCGACTTCTAAAAAGCAATCCGCAAAAAGGGGCTGCCCTGCAGCCCCTCGCTCCTTCAGCTCCGCTCCCGCACCCAGAACAACGTAGCCCCGGCCACTGCCGCCGGca < 8:79533/131‑1 (MQ=255) cACCCTCGAACCCTACAGTTTCTCCGACTTCTAAAAAGCAATCCGCAAAAAGGGGCTGCCCTGCAGCCCCTCGCTCCTTCAGCTCCGCTCCCGCACCCAGAACAACGTAGCCCCGGCCACTGCCGCCGGca > 7:79533/1‑131 (MQ=255) gAACCCTACAGTTTCTCCGACTTCTAAAAAGCAATCCGCAAAAAGGGGCTGCCCTGCAGCCCCTCGCTCCTTCAGCTCCGCTCCCGCACCCAGAACAACGTAGCCCCGGCCACTGCCGCCGGCATCATCAGCACGTTCACGCCTGGaa < 7:207212/148‑1 (MQ=255) ccTACAGTTTCTCCGACTTCTAAAAAGCAATCCGCAAAAAGGGGCTGCCCTGCAGCCCCTCGCTCCTTCAGCTCCGCTCCCGCACCCAGAACAACGTAGCCCCGGCCACTGCCGCCGGCATCATCAGCACGTTCACGCCCGGAatcatc < 5:155357/149‑1 (MQ=255) cTACAGTTTCTCCGACTTCTAAAAAGCAATCCGCAAAAAGGGGCTGCCCTGCAGCCCCTCGCTCCTTCAGCTCCGCTCCCGCACCCAGAACAACGTAGCCCCGGCCACTGCCGCCGGCATCATCAGCACGTTCACGCCTGGAAtcatca < 2:29179/149‑1 (MQ=255) ctcCGACTTCTAAAAAGCAATCCGCAAAAAGGGGCTGCCCTGCAGCCCCTCGCTCCTTCAGCTCCGCTCCCGCACCCAGAACAACGTAGCCCCGGCCACTGCCGCCGGCATCATCAGCACGTTCACGCCTGGAATCATCAGCGCCagg < 6:12127/148‑1 (MQ=255) ttCTAAAAAGCAATCCGCAAAAAGGGGCTGCCCTGCAGCCCCTCGCTCCTTCAGCTCCGCTCCCGCACCCAGAACAACGTAGCCCCGGCCACTGCCGCCGGCATCATCAGCACGTTCACGCCTGGAATCATCAGCGCCa > 4:6516/1‑139 (MQ=255) tAAAAAGCAATCCGCAAAAAGGGGCTGCCCTGCAGCCCCTCGCTCCTTCAGCTCCGCTCCCGCACCCAGAACAACGTAGCCCCGGCCACTGCCGCCGGCATCATCAGCACGTTCACGCCTGGAATCATCAGCGCCAGGTAGGTGATgcc > 8:165319/1‑149 (MQ=255) aaaaaGCAATCCGCAAAAAGGGGCTGCCCTGCAGCCCCTCGCTCCTTCAGCTCCGCTCCCGCACCCAGAACAACGTAGCCCCGGCCACTGCCGCCGGCATCATCAGCACGTTCACGCCTGGAATCATCAGCGCCagg > 5:5280/1‑137 (MQ=255) aaaaaGCAATCCGCAAAAAGGGGCTGCCCTGCAGCCCCTCGCTCCTTCAGCTCCGCTCCCGCACCCAGAACAACGTAGCCCCGGCCACTGCCGCCGGCATCATCAGCACGTTCACGCCTGGAATCATCAGCGCCagg < 6:5280/137‑1 (MQ=255) aaaaaGCAATCCGCAAAAAGGGGCTGCCCTGCAGCCCCTCGCTCCTTCAGCTCCGCTCCCGCACCCAGAACAACGTAGCCCCGGCCACTGCCGCCGGCATCATCAGCACGTTCACGCCTGGAATCATCAGCGCCAGGTAGGTGATgccg < 7:82720/149‑1 (MQ=255) aaaaGCAATCCGCAAAAAGGGGCTGCCCTGCAGCCCCTCGCTCCTTCAGCTCCGCTCCCGCACCCAGAACAACGTAGCCCCGGCCACTGCCGCCGGCATCATCAGCACGTTCACGcc < 4:61417/117‑1 (MQ=255) aaaaGCAATCCGCAAAAAGGGGCTGCCCTGCAGCCCCTCGCTCCTTCAGCTCCGCTCCCGCACCCAGAACAACGTAGCCCCGGCCACTGCCGCCGGCATCATCAGCACGTTCACGcc > 3:61417/1‑117 (MQ=255) aGCAATCCGCAAAAAGGGGCTGCCCTGCAGCCCCTCGCTCCTTCAGCTCCGCTCCCGCACCCAGAACAACGTAGCCCCGGCCACTGCCGCCGGCATCATCAGCACGTTCACGCCTGGAATCATCAGCGCCAGGTAGGTGATGCCGCCaa < 6:251958/149‑1 (MQ=255) gCAAAAAGGGGCTGCCCTGCAGCCCCTCGCTCCTTCAGCTCCGCTCCCGCACCCAGAACAACGTAGCCCCGGCCACTGCCGCCGGCATCATCAGCACGTTCACGCCTGGAATCATCAGCGCCAGGTAGGTGATGCCGCCAAAGCCCAgg > 2:113331/1‑149 (MQ=255) gCAAAAAGGGGCTGCCCTGCAGCCCCTCGCTCCTTAAGCTCCGCTCCCGCACCCAGAACAACGTAGCCCCGGCCACTGCCGCCGGCATCATCAGCACGTTCAAGCCTGGAATCATCAGCGCCAGGTAGGTGATGCCGCCAAAGCCCAgg > 2:136055/1‑149 (MQ=255) gaGGGGCTGCAGGGCAGCCCCTCGCTCCTTCAGCTCCGCTCCCGCACCCAGAACAACGTAGCCCCGGCCACTGCCGCCGGCATCATCAGCACGTTCACGCCTGGAATCATCAGCGCCAGGTAGGTGATGCCGCCAAAGCCCAGGCTCTg > 5:230362/2‑149 (MQ=255) aGGGGCTGCAGGGCAGCCCCTCGCTCCTTCAGCTCCGCTCCCGCACCCAGAACAACGTAGCCCCGGCCACTGCCGCCGGCATCATCAGCACCTTCACGCCTGCAATCATCAGCGCCAGGTAGGTGATGACCCCAAAGCCCACGctctcc > 2:67095/1‑147 (MQ=255) ggCTGCCCTGCAGCCCCTCGCTCCTTCAGCTCCGCTCCCGCACCCAGAACAACGTAGCCCCGGCCACTGCCGCCGGCATCATCAGCACGTTCACGCCTGGAATCATCAGCGCCAGGTAGGTGATGCCGCCAAAGCCCAGGCTCTGCCAg > 4:86973/1‑149 (MQ=255) tGCCCTGCAGCCCCTCGCTCCTTCAGCTCCGCTCCCGCACCCAGAACAACGTAGCCCCGGCCACTGCCGCCGGCATCATCAGCACGTTCACGCCTGGAATCATCAGCGCCAGGTAGGTGATGCCGCCAAAGCCCAGGCTCTGCCAgcgc > 5:171193/1‑149 (MQ=255) cccTGCAGCCCCTCGCTCCTTCAGCTCCGCTCCCGCACCCAGAACAACGTAGCCCCGGCCACTGCCGCCGGCATCATCAGCACGTTCACGCCTGGAatc > 7:40874/1‑99 (MQ=255) cccTGCAGCCCCTCGCTCCTTCAGCTCCGCTCCCGCACCCAGAACAACGTAGCCCCGGCCACTGCCGCCGGCATCATCAGCACGTTCACGCCTGGAatc < 8:40874/99‑1 (MQ=255) cccTGCAGCCCCTCGCTCCTTCAGCTCCGCTCCCGCACCCAGAACAACGTAGCCCCGGCCACTGCCGCCGGCATCATCAGCACGTTCACGCCTGGAATCATCAGCGCCAGGTAGGTGATGCCGCCAAAGCCCAGGCTCTGCCAGCGCtt > 2:216725/1‑149 (MQ=255) ccTGCAGCCCCTCGCTCCTTCAGCTCCGCTCCCGCACCCAGAACAACGTAGCCCCGGCCACTGCCGCCGGCATCATCAGCACGTTCACGCCTGGAATCATCAGCGCCAGGTAGGTGATGCCGCCAAAGCCCAGGCTCTGCCAGCGCTTc > 7:90881/1‑149 (MQ=255) tGCAGCCCCTCGCTCCTTCAGCTCCGCTCCCGCACCCAGAACAACGTAGCCCCGGCCACTGCCGCCGGCATCATCAGCACGTTCACGCCTGGAATCATCAGCGCCAGGTAGGTGATGCCGCCAAAGCCCAGGCTCTGCCAGCGCTTCTg > 4:48429/1‑149 (MQ=255) | GGCGTTCGGCGGGTCAAGCGACCTTGCTAACCTCGAGAAGGTTAAGGCAGTGGAACATCTAATACTGCTTTCTCAGATTGCCACCCTCGAACCCTACAGTTTCTCCGACTTCTAAAAAGCAATCCGCAAAAAGGGGCTGCCCTGCAGCCCCTCGCTCCTTCAGCTCCGCTCCCGCACCCAGAACAACGTAGCCCCGGCCACTGCCGCCGGCATCATCAGCACGTTCACGCCTGGAATCATCAGCGCCAGGTAGGTGATGCCGCCAAAGCCCAGGCTCTGCCAGCGCTTCTG > NC_002947/900394‑900684 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |