Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 955,149 A→G 13.3% intergenic (+103/‑210) cyoE → / → alaC protoheme IX farnesyltransferase/aminotransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947955,1490AG13.3% 93.3 / 6.6 39intergenic (+103/‑210)cyoE/alaCprotoheme IX farnesyltransferase/aminotransferase
Reads supporting (aligned to +/- strand):  ref base A (16/17);  new base G (2/3);  total (18/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.17e-01

GACAGCCAGACCGCTGCGGATGTGCTGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGGTTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAGGTTCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCGGCACTATCTTCTGA  >  NC_002947/955002‑955292
                                                                                                                                                   |                                                                                                                                               
gACAGCCAGACCGCTGCGGATGTGCTGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGGTTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAg                                                                                                                                                >  5:91297/1‑149 (MQ=255)
gACAGCCAGACCGCTGCGGATGTGCTGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGGTTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAg                                                                                                                                                >  4:84518/1‑149 (MQ=255)
   aGCCAGACCGCTGCGGATGTGCTGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGGTTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAGGt                                                                                                                                              >  6:127425/1‑148 (MQ=255)
        gACCGCTGCGGATGTGCTGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGGTTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTTAACCCGCTGCAACAATGTTACTGt                                                                                                                                        >  1:68581/1‑149 (MQ=255)
             cTGCGGATGTGCTGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGGTTTTTTTTTGGGGGTATTTTTCCTGTGCTGGCCTCTTCGCGGGTAAACCCGCTCCTACAGGAACCTTGTAGGAg                                                                                                                                   <  1:12116/149‑1 (MQ=37)
                cGGATGTGCTGATGTCCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGTTTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAGGTTCCTGTAGGAGCgg                                                                                                                                <  6:103384/149‑1 (MQ=255)
                cGGATGTGCTGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGGTTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAGGTTCCTGTAGGAGc                                                                                                                                  <  2:144121/147‑1 (MQ=255)
                 ggATGTGCTGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGGTTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAGGTTCCTGTAGGAGCggg                                                                                                                               <  7:76242/149‑1 (MQ=255)
                 atctgtgCTGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGGTTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAGGTTCCTGTAGGAGCggg                                                                                                                               <  5:264288/146‑1 (MQ=255)
                  gctgtgCTGATGACCTACCCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGGTTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAGGTTCCTGTAGGAGCGGGt                                                                                                                              <  2:214081/147‑1 (MQ=255)
                      tgCTGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGGTTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAGGTTCCTGTAGGAGCggg                                                                                                                               >  6:264288/1‑144 (MQ=255)
                      tgCTGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGGTTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAGGTTCCTGTAGGAGCGGGTTTa                                                                                                                           <  5:38285/148‑1 (MQ=255)
                        cTGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGGTTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAGGTTCCTGTAGGAGCGGGTTTAccc                                                                                                                        <  6:91297/149‑1 (MQ=255)
                           atgaCCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGGTTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAGGTTCCTg                                                                                                                                         <  6:90952/129‑1 (MQ=255)
                           atgaCCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGGTTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAGGTTCCTg                                                                                                                                         >  5:90952/1‑129 (MQ=255)
                                cTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGGTTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAGGTTCCTGTAGGAGCGGGTTTACCCGCGAagag                                                                                                                <  8:80821/149‑1 (MQ=255)
                                 tACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGGTTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAGGAACCTTGTAGGAGCgg                                                                                                                                >  6:62412/1‑132 (MQ=37)
                                 tACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGGTTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAGGAACCTTGTAGGAGCgg                                                                                                                                <  5:62412/132‑1 (MQ=37)
                                    gcacgcTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGGTTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAAACCCCAACTACAAGGTTACAGGTGGCGCGGGGTTAACCGCGAAAAggg                                                                                                              >  4:228675/1‑146 (MQ=255)
                                     cacgcTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGGTTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTTAAACCCCGCCGAAAAAGTTGCGGTTGGGGCGGGGTTTCCCCCGCACAGGGCa                                                                                                            >  1:94235/1‑148 (MQ=255)
                                           gAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGGTTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAAACCGCTCCTACAAGGTTACTGTTGGAGCGGGTTTACCCGCGAAGAGGCCAGCCCAg                                                                                                      >  3:200491/1‑148 (MQ=255)
                                                       tGTTTCACGAAAACCCCGGCCACTGTGCCGGGGTTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCCCTACAAAAAAGTTCCTGTAGGAGCGGGTTTAACCCCGAAGAGGCCAGCACAGaaaaaaaaaaat                                                                                          >  6:271248/1‑148 (MQ=38)
                                                                    ccccGGCCACTGTGCCGGGGTTTTTTTTTGGGGGTATTTTT‑CTGTGCTGGCCTCTTCGCGGGTAAACCCGCTCCTACAGGAACCTTGTAGGAGCgg                                                                                                                                >  3:123738/1‑96 (MQ=14)
                                                                    ccccGGCCACTGTGCCGGGGTTTTTTTTTGGGGGTATTTTT‑CTGTGCTGGCCTCTTCGCGGGTAAACCCGCTCCTACAGGAACCTTGTAGGAGCgg                                                                                                                                <  4:123738/96‑1 (MQ=14)
                                                                                        tttttttttGGGGTTTTTTTTCCTGTGCCGGCCTATTCGCGGTAAAACCCGCTCATAAAAGGTTCCTGTAGGAGCGGTTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTc                                                         <  3:263662/148‑1 (MQ=38)
                                                                                          tttttttGGGTTTATTTTTCGTGTGCCGGCTTGTTCGGGGTTAAACCGGTTCCTACATGGTTCTTTTTGTTGGGGTTTTACACGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCta                                                       <  8:166479/148‑1 (MQ=255)
                                                                                          tttttttGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCATACAAGTTTCCTGTAGTAGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCta                                                       <  3:228675/148‑1 (MQ=255)
                                                                                                    ggTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAGGTTCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGa                                            <  5:127425/149‑1 (MQ=255)
                                                                                                                   ccGGCCTCTTCGCGGGTAAACCCGCTCCTACAAGGTTCCTTTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACa                              <  5:72332/148‑1 (MQ=255)
                                                                                                                           ttCGCGGGTAAACCCGCTCCTACAAGGTTCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACa                                               <  6:127490/123‑1 (MQ=255)
                                                                                                                           ttCGCGGGTAAACCCGCTCCTACAAGGTTCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACa                                               >  5:127490/1‑123 (MQ=255)
                                                                                                                              gcgGGTAAACCCGCTCCTACAAGGTTCCTTTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGttt                  <  4:200491/149‑1 (MQ=255)
                                                                                                                                   tAAACCCGCTCCTACAAGGTTCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCGGCa             <  2:68581/149‑1 (MQ=255)
                                                                                                                                      aCCCGCTCCTACAAGGTTCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCGGCACTa          >  7:235744/1‑149 (MQ=255)
                                                                                                                                        ccGCTCCTACAAGGTTCCTGTAGGAGCGGGTTTACACGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAAGAGGATATTATCCTTTACAGGCATCGTTTTTCGTCACTTtc        >  3:29740/1‑149 (MQ=39)
                                                                                                                                          gCTCCTACAAGGTTCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGATCTAATAAAACAGGAACTTATCCTTTACAGGCATCCTGTTTCGGCACTAtct       >  8:103621/1‑148 (MQ=255)
                                                                                                                                            tCCTACAAGGTTCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTc                 >  7:164152/1‑136 (MQ=255)
                                                                                                                                            tCCTACAAGGTTCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTc                 <  8:164152/136‑1 (MQ=255)
                                                                                                                                              cTACAAGGTTCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCGGCACTATCTACTGa  >  5:215655/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                               
GACAGCCAGACCGCTGCGGATGTGCTGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGGTTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAGGTTCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCGGCACTATCTTCTGA  >  NC_002947/955002‑955292

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑