Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 127,123 G→C 12.6% intergenic (‑33/+37) thrB ← / ← PP_0122 homoserine kinase/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947127,1230GC12.6% 118.6 / 10.5 48intergenic (‑33/+37)thrB/PP_0122homoserine kinase/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (20/21);  new base C (3/3);  total (24/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.96e-01

AAATTGCTGTTCTCGGTACCGGCGGCAATGCCCTGGAAGTCGAGCAGACGGCCCAGCTCGTACGGCGCCAGAAAGGTTTCCAGCTCAGGCCGGGTCACGGGGGTGAAGACTGACATGATGAAAAATTGCCCATACGGGCACTTCCGCCGGGAAGTGCCGGGTTGATGTGAACGGTGCGCGTCAGCTTACCACTCGAAGATCTTCCAGGACGGAATCAGCATGTCCGGCTGGTCGGATCGAATGAAATTGGCATCAGTGCCATCAGCGCGTACCAGAAAATAAGGCT  >  NC_002947/126975‑127260
                                                                                                                                                    |                                                                                                                                         
aaaTTGCTGTTCTCGGTACCGGCGGCAATGCCCTGGAAGTCGAGCAGACGGCCCAGCTCGTACGGCGCCAGAAAGGTTTCCAGCTCAGGCCGGGTCACGGGGGTGAAGACTGACATGATGAAAAATTGCCCATACGGGCACTTccgccg                                                                                                                                           >  7:210562/1‑149 (MQ=255)
     gCTGTTCTCGGTACCGGCGGCAATGCCCTGGAAGTCGAGCAGACGGCCCAGCTCGTACGGCGCCAGAAAGGTTTCCAGCTCAGGCCGGGTCACGGGGGTGAAGACTGACATGATGAAAAATTGCCCATACGGGCACTTCCGCCGGGAAg                                                                                                                                      >  2:80131/1‑149 (MQ=255)
         ttCTCGGTACCGGCGGCAATGCCCTGGAAGTCGAGCAGACGGCCCAGCTCGTACGGCGCCAGAAAGGTTTCCAGCTCAGGCCGGGTCACGGGGGTGAAGACTGACATGATGAAAAATTGCCCATACGGGCACTTCCCGGCGGAAGTGcc                                                                                                                                  <  2:100732/149‑1 (MQ=255)
         ttCTCGGTACCGGCGGCAATGCCCTGGAAGTCGAGCAGACGGCCCAGCTCGTACGGCGCCAGAAAGGTTTCCAGCTCAGGCCGGGTCACGGGGGAGAAGACTGACATGATGAAAAATTGCCCATACGGGCACTTCCGCCGGGAAGTGcc                                                                                                                                  >  7:239810/1‑149 (MQ=255)
           ctcGGTACCGGCGGCAATGCCCTGGAAGTCGAGCAGACGGCCCAGCTCGTACGGCGCCAGAAAGGTTTCCAGCTCAGGCCGGGTCACGGGGGTGAAGACTGACATGATGAAAAATTGCCCATACGGGCACTTCCGCCGGGAAGTGCCgg                                                                                                                                >  3:241500/1‑149 (MQ=255)
            tcGGTACCGGCGGCAATGCCCTTGAAGTCGAGCAGACGGCCCAGCTCGTACGGCGCCAGAAAGGTTTCCAGCTCAGGCCGGGTCACTGGGGTGAAGACTGACATGATGAAAAATTGCCCATACGGGCACTTCCGCCGGGAAGTGCCgg                                                                                                                                <  8:210562/148‑1 (MQ=255)
              ggTACCGGCGGCAATGCCCTGGAAGTCGAGCAGACGGCCCAGCTCGTACGGCGCCAGAAAGGTTTCCAGCTCAGGCCGGGTCACGGGGGTGAAGACTGACATGATGAAAAATTGCCCATACGGGCACTTCCGCCGGGAAGTGCCGGGtt                                                                                                                             <  4:241500/149‑1 (MQ=255)
              gctACCGGCGGCAATGCCCTGGAAGTCGAGCAGACGGCCCAGCTCGTACGGCGCCAGAAAGGTTTCCAGCTCAGGCCGGGTCACGGGGGTGATGACTGACAAGATGAAAAATTGCCCATACCGCCACTTCCCGGCGGAAGTGcccgtat                                                                                                                             <  1:207923/147‑6 (MQ=255)
                    ggcggcAATGCCCTGGAAGTCGAGCAGACGGCCCAGCTCGTACGGCGCCAGAAAGGTTTCCAGCTCAGGCCGGGTCACGGGGGTGAAGACTGACATGATGAAAAATTGCCCATACGGGCACTTCCGCCGGGAAGTGCCGGGTTGAtgtg                                                                                                                       <  1:131563/149‑1 (MQ=255)
                             gCCCTGGAAGTCGAGCAGACGGCCCAGCTCGTACGGCGCCAGAAAGGTTTCCAGCTCAGGCCGGGTCACGGGGGTGAAGACTGACATGATGAAAAATTGCCCATACGGGCACTTCTGCCGGGAAGTGCCGGGTTGATGTGAACGGTgcg                                                                                                              >  5:155974/1‑149 (MQ=255)
                                  ggAAGTCGAGCAGACGGCCCAGCTCGTACGGCGCCAGAAAGGTTTCCAGCTCAGGCCGGGTCACGGGGGTGAAGACTGACATGATGAAAAATTGCCCATACGGGCACTTCCGCCGGGAAGTGCCGGGTTGAt                                                                                                                          >  6:70854/1‑132 (MQ=255)
                                  ggAAGTCGAGCAGACGGCCCAGCTCGTACGGCGCCAGAAAGGTTTCCAGCTCAGGCCGGGTCACGGGGGTGAAGACTGACATGATGAAAAATTGCCCATACGGGCACTTCCGCCGGGAAGTGCCGGGTTGAt                                                                                                                          <  5:70854/132‑1 (MQ=255)
                                   gAAGTCGAGCAGACGGCCCAGCTCGTACGGCGCCAGAAAGGTTTCCAGCTCAGGCCGGGTCACGGGGGTGAAGACTGACATGATGAAAAATTGCCCATACGGGCACTTCCGCCGGGAAGTGCCGGGTTGATGTGAACGGTGCGCGt                                                                                                           >  3:24633/1‑146 (MQ=255)
                                   gAAGTCGAGCAGACGGCCCAGCTCGTACGGCGCCAGAAAGGTTTCCAGCTCAGGCCGGGTCACGGGGGTGAAGACTGACATGATGAAAAATTGCCCATACGGGCACTTCCGCCGGGAAGTGCCGGGTTGATGTGAACGGTGCGCGTCa                                                                                                         <  4:24633/148‑1 (MQ=255)
                                   gAAGTCGAGCAGACGGCCCAGCTCGTACGGCGCCAGAAAGGTTTCCAGCTCAGGCCGGGTCACGGGGGTGAAGACTGACATGATGAAAAATTGCCCATACGGGCACTTCCGCCGGGAAGTGCCGGGTTGATGTGAACGGTGCGCGTCAg                                                                                                        >  5:27415/1‑149 (MQ=255)
                                    aaGTCGAGCAGACGGCCCAGCTCGTAGGGCGCCAGAAAGGTTTCCAGCTCAGGCCGGGTCACGGGGGTGAAGACTGACATGATGAAAAATTGCCCATACGGGCACTTCCGCCGGGAAGTGCCGGGTTGATGTGAACGGTGCGCGTCAGc                                                                                                       <  7:199463/149‑1 (MQ=255)
                                     aGTCGAGCAGACGGCCCAGCTCGTACGGCGCCAGAAAGGTTTCCAGCTCAGGCCGGGTCACGGGGGTGAAGACTGACATGATGAAAAATTGCCCATACGGGCACTTCCGCCGGGAAGTGCCGGGTTGATGTGAACGGTGCGCGTCGGCt                                                                                                      <  1:80131/149‑1 (MQ=255)
                                       tCGAGCAGACGGCCCAGCTCGTACGGCGCCAGAAAGGTTTCCAGCTCAGGCCGGGTCACGGGGGTGAAGACTGACATGATGAAAAATTGCCCATACGGGCACTTCCGCCGGGAAGTGCCGGGTTGATGTGAACGGTGCGCGTCAGCTTa                                                                                                    <  3:118102/149‑1 (MQ=255)
                                         gAGCAGACGGCCCAGCTCGTACGGCGCCAGAAAGGTTTCCAGCTCAGGCCGGGTCACGGGGGTGAAGACTGACATGATGAAAAATTGCCCATACGGGCACTTCCGCCGGGAAGTGCCGGGTTGATGTGAACGGTGCGCGTCAGCTTAc                                                                                                   <  6:176933/148‑1 (MQ=255)
                                              gACGGCCCAGCTCGTACGGCGCCAGAAAGGTTTCCAGCTCAGGCCGGGTCACGGGGGTGAAGACTGACATGATGAAAAATTGCCCATACGGGCACTTCCGCCGGGAAGTGCCGGGTTGATGTGAACGGTGCGCGTCAGCTTACCACTc                                                                                              >  8:244961/1‑148 (MQ=255)
                                               aCGGCCCAGCTCGTACGGCGCCAGAAAGGTTTCCAGCTCAGGCCGGGTCACGGGGGTGAAGACTGACATGATGAAAAATTGCCCATACGGGCACTTCCGCCGGGAAGTGCCGGGTTGATGTGAACGGTGCGCGTCAGCTTACCACTCg                                                                                             >  7:117380/1‑148 (MQ=255)
                                                       gCTCGTACGGCGCCAGAAAGGTTTCCAGCTCAGGCCGGGTCACGGGGGTGAAGACTGACATGATGAAAAATTGCCCATACGGGCACTTCCGCCGGGAAGTGCCGGGTTGATGTGAACGGTGCGCGTCAGCTTACCACTCGAAGATCTTc                                                                                    <  7:204576/149‑1 (MQ=255)
                                                       gCTCGTACGGCGCCAGAAAGGTTTCCAGCTCAGGCCGGGTCACGGGGGTGAAGACTGACATGATGAAAAATTGCCCATACGGGCACTTCCGCCGGGAAGTGCCGGGTTGATGTGAACGGTGCGCGTCAGCTTACCACTCGAAGATCTTc                                                                                    <  5:253112/149‑1 (MQ=255)
                                                          cGTACGGCGCCAGAAAGGTTTCCAGCTCAGGCCGGGTCACGGGGGTGAAGACTGACATGATGAAAAATTGCCCATACGGGCACTTCCGCCGGGAAGTGCCGGGTTGATGTGAACGGTGCGCGTCAGCTTACCACTCGAAGATCTTCCAg                                                                                 >  7:137089/1‑149 (MQ=255)
                                                           gTACGGCGCCAGAAAGGTTTCCAGCTCAGGCCGGGTCACGGGGGTGAAGACTGACATGATGAAAAATTGCCCATACGGGCACTTCCGCCGGGAAGTGCCGGGTTGATGTGAACGGTGCGCGTCAGCTTACCACTCGAAGATCTTCCa                                                                                  <  8:233181/147‑1 (MQ=255)
                                                           gTACGGCGCCAGAAAGGTTTCAAGCTCAGGCCGGGTCACGGGGGTGAAGACTGACATGATGAAAAATTGCCAATACGGGCACTTCCGCCGGGAAGTGCCGGGTTGATGTGAACGGTTCGCTTCAGCTTACAAATCGAAGATCTTCCAg                                                                                 >  8:190155/1‑148 (MQ=255)
                                                              cGGCGCCAGAAAGGTTTCCAGCTCAGGCCGGGTCACGGGGGTGAAGACTGACATGATGAAAAATTGCCCATACGGGCACTTCAGCCGGGAAGTGCCGGGTTGATGTGAACGGTGCGCGTCAGCTTACCACTCGAAGATCTTCCAGGACg                                                                             <  6:155974/149‑1 (MQ=255)
                                                                gcgcCAGAAAGGTTTCCAGCTCAGGCCGGGTCACGGGGGTGAAGACTGACATGATGAAAAATTGCCCATACGGGCACTTCCGCCGGGAAGTGCCGGGTTGATGTGAACGGTGCGCGTCAGCTTACCACTCGAAGATCTTCCAGGACGGa                                                                           <  8:137089/149‑1 (MQ=255)
                                                                        aaGGTTTCCAGCTCAGGCCGGGTCACGGGGGTGAAGACTGACATGATGAAAAATTGCCCATACGGGCACTTCCGCCGGGAAGTGCCGGGTTGATGTGAACGGTGCGCGTCAGCTTACCACTCGAAGATCTTCCAGGACGGAATCAGCa                                                                    <  6:27415/148‑1 (MQ=255)
                                                                                cAGCTCAGGCCGGGTCACGGGGGTGAAGACTGACATGATGAAAAATTGCCCATACGGGCACTTCCGCCGGGAAGTGCCGGGTTGATGTGAACGGTGCGCGTCAGCTTACCACTCGAAGATCTTCCAGGACGGAATCAGCATGTCCGGCt                                                           >  5:272336/1‑149 (MQ=255)
                                                                                        gCCGGGTCACGGGGGTGAAGACTGACATGATGAAAAATTGCCCATACGGGCACTTCCGCCGGGAAGTGCCGGGTTGATGTGAACGGTGCGCGTCAGCTTACCACTCGAAGATCTTCCAGGAGGGAATCAGCATGTCAGGCTGGTCGGAt                                                   >  7:91107/1‑149 (MQ=255)
                                                                                                        gAAGACTGACATGATGAAAAATTGCCCATACGGGCACTTCCGCCGGGAAGTGCCGGGTTGATGTGAACGGTGCGCGTCAGCTTACCACTCGAAGATCTTCCAGGACGGAATCAGCATGTCCGGCTGGTCGGATCGAATGAAATTGGCAt                                   >  5:143344/1‑149 (MQ=255)
                                                                                                             cTGACATGATGAAAAATTGCCCATACGGGCACTTCCGCCGGGAAGTGCCGGGTTGATGTGAACGGTGCGCGTCAGCTTACCACTCGAAGATCTTCCAGGACGGAATCAGCATGTCCGGCTGGTCGGATCGAATGAAATTGGCATCAGTg                              <  8:117380/149‑1 (MQ=255)
                                                                                                                     atgaAAAATTGCCCATACGGGCACTTCCGCCGGGAAGTGCCGGGTTGATGTGAACGGTGCGCGTCAGCTTACCACTCGAAGATCTTCCAGGACGGAATCAGCATGTCCGGCTGGTCGGATCGAATGAAATTGGCATCAGTGCCATCAg                       <  7:244961/148‑1 (MQ=255)
                                                                                                                      tgaAAAATTGCCCATACGGGCACTTACGCCGGGAAGTGCCGGGTTGATGTGAACGGTGCGCGTCAGCTTACCACTCGAAGATCTTCCAGGACGGAATCAGCATGTCCGGCTGGTCGGATCGAATGAAATTGGCATCAGTGCCATCAgc                      <  7:190155/148‑1 (MQ=255)
                                                                                                                       gaAAAATTGCCCATACGGGCACTTCCGCCGGGAAGTGCCGGGTTGATGTGAACGGTGCGCGTCAGCTTACCACTCGAAGATCTTCCAGGACGGAATCAGCATGTCCGGCTGGTCGGATCGAATGAAATTGGCATCAGTGCCATCAgcgc                    >  8:234493/1‑149 (MQ=255)
                                                                                                                                cccATACGGGCACTTCCGCCGGGAAGTGCCGGGTTGATGTGAACGGTGCGCGTCAGCTTACCACTCGAAGATCTTCCAGGACGGAATCAGCATGTCCGGCTGGTCGGATCGAATGAAAtt                                        >  4:164254/1‑120 (MQ=255)
                                                                                                                                cccATACGGGCACTTCCGCCGGGAAGTGCCGGGTTGATGTGAACGGTGCGCGTCAGCTTACCACTCGAAGATCTTCCAGGACGGAATCAGCATGTCCGGCTGGTCGGATCGAATGAAAtt                                        <  3:164254/120‑1 (MQ=255)
                                                                                                                                 ccATACGGGCACTTCCGCCGGGAAGTGCCGGGTTGATGTGAACGGTGCGCGTCAGCTTACCACTCGAAGATCTTCCAGGACGGAATCAGCATGTACGGCTGGTCGGATCGAATGAAATTGGCATCACTGCAAt                          >  8:190068/1‑133 (MQ=255)
                                                                                                                                 ccATACGGGCACTTCCGCCAGGAAGTGCCGGGTTGATGTGAACGGTGCGCGTCAGCTTACCACTCGAAGATCTTCCAGGACGGAATCAGCATGTCCGGCTGGTCGGATCGAATGAAATTGGCATCAGTGCCATCAGCGCGTACCAGaa           >  3:258731/1‑148 (MQ=255)
                                                                                                                                   aTACGGGCACTTCCGCCGGGAAGTGCCGGGTTGATGTGAACGGTGCGCGTCAGCTTACCACTCGAAGATCTTCCAGGACGGAATCAGCATGTCCGGCTGGTCGGATCGAAt                                              >  5:62071/1‑111 (MQ=255)
                                                                                                                                   aTACGGGCACTTCCGCCGGGAAGTGCCGGGTTGATGTGAACGGTGCGCGTCAGCTTACCACTCGAAGATCTTCCAGGACGGAATCAGCATGTCCGGCTGGTCGGATCGAAt                                              <  6:62071/111‑1 (MQ=255)
                                                                                                                                   aTACGGGCACTTCCGCCGGGAAGTGCCGGGTTGATGTGAACGGTGCGCGTCAGCTTACCACTCGAAGATCTTCCAGGACGGAATCAGCATGTCCGGCTGGTCGGATCGAATGAAATTGGCATCAGTGCCATCAGCGCGTACCAGaaaa         >  1:76296/1‑148 (MQ=255)
                                                                                                                                      ccggCACTTCCCGGCGGAAGTGCCGGGTTGATGTGAACGGTGCGCGTCAGCTTACCACTCGAAGATCTTCCAGGACGGAATCAGCATGTCCGGCTGGTCGGATCGAATGAAATTGGCATCAGTGCCATCAGCGCGTACCAGAAAATAAg     >  7:57542/3‑149 (MQ=255)
                                                                                                                                      cGGGCACTTCCGCCGGGAAGTGCCGGGTTGATGTGAACGGTGCGCGTCAGCTTACCACTCGAAGATCTTCCAGGACGGAATCAGCATGTCCGGCTGGTCGGATCGAATGAAATTGGCATCAGTGCCATCAGCGCGTACCAGAAAATaa      <  8:239810/148‑1 (MQ=255)
                                                                                                                                       cggCACTTCCCGGCGGAAGTGCCGGGTTGATGTGAACGGTGCGCGTCAGCTTACCACTCGAAGATCTTCCAGGACGGAATCAGCATGTCCGGCTGGTCGGATCGAATGAAATTGGCATCAGTGCCATCAGCGCGTACCAGaaa          <  7:251813/142‑1 (MQ=255)
                                                                                                                                       cggCACTTCCCGGCGGAAGTGCCGGGTTGATGTGAACGGTGCGCGTCAGCTTACCACTCGAAGATCTTCCAGGACGGAATCAGCATGTCCGGCTGGTCGGATCGAATGAAATTGGCATCAGTGCCATCAGCGCGTACCAGaaa          >  8:251813/2‑143 (MQ=255)
                                                                                                                                       cggCACTTCCCGGCGGAAGTGCCGGGTTGATGTGAACGGTGCGCGTCAGCTTACCACTCGAAGATCTTCCAGGACGGAATCAGCATGTCCGGCTGGTCGGATCGAATGAAATTGGCATCAGTGCCATCAGCGCGTACCAGAAAATAAgg    >  6:53780/2‑149 (MQ=255)
                                                                                                                                         gCACTTCCGCCGGGAAGTGCCGGGTTGATGTGAACGGTGCGCGTCAGCTTACCACTCGAAGATCTTCCAGGACGGAATCAGCATGTCCGGCTGGTCGGATCGAATGAAATTGGCATCAGTGCCATCAGCGCGTACCAGAAAATAAGGCt  >  5:36830/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                         
AAATTGCTGTTCTCGGTACCGGCGGCAATGCCCTGGAAGTCGAGCAGACGGCCCAGCTCGTACGGCGCCAGAAAGGTTTCCAGCTCAGGCCGGGTCACGGGGGTGAAGACTGACATGATGAAAAATTGCCCATACGGGCACTTCCGCCGGGAAGTGCCGGGTTGATGTGAACGGTGCGCGTCAGCTTACCACTCGAAGATCTTCCAGGACGGAATCAGCATGTCCGGCTGGTCGGATCGAATGAAATTGGCATCAGTGCCATCAGCGCGTACCAGAAAATAAGGCT  >  NC_002947/126975‑127260

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑