Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 983,256 T→C 6.6% V89V (GTT→GTC)  fdx → ferredoxin

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947983,2560TC 6.6% 220.2 / 4.8 75V89V (GTT→GTC) fdxferredoxin
Reads supporting (aligned to +/- strand):  ref base T (32/38);  new base C (3/2);  total (35/40)
Fisher's exact test for biased strand distribution p-value = 6.59e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.14e-01

CCTGCGGCGGCGTCAAGGCCTGTACCACTTGCCACTGCATCGTGCGCAAGGGCTTCGATTCGCTCGAAGAGGCTGACGAGCTGGAAGAGGACATGCTGGACAAAGCCTGGGGCCTGGAAGCGCAATCGCGCCTGGGTTGCCAGGTTTTCGTCGCTGACGAAGACCTGACCATCGAGATCCCCAAGTATTCGCTCAACCATGCTGCCGAAGCACCGCACTGAGGGCCTGTACATGAGTCTGAAATGGGTTGATGTACTTGAGATCGCAATCCAGCTTGCAGAAAGCAAGC  >  NC_002947/983111‑983399
                                                                                                                                                 |                                                                                                                                               
ccTGCGGCGGCGTCAAGGCCTGTACCACTTGCCACTGCATCGTGCGCAAGGGCTTCGATTCGCTCGAAGAGGCTGACGAGCTGGAAGAGGACATGCTGGACAAAGCCTGGGGCCTGGAAGCGCAATCGCGCCTGGGTTGCCAGGTTTtc                                                                                                                                              <  1:111404/149‑1 (MQ=255)
      gcggcgTCAAGGCCTGTACCACTTGCCACTGCATCGTGCGCAAGGGCTTCGATTCGCTCGAAGAGGCTGACGAGCTGGAAGAGGACATGCTGGACAAAGCCTGGGGCCTGGAAGCGCAATCGCGCCTGGGTTGCCAGGTTTTCGTCGCt                                                                                                                                        >  1:33101/1‑149 (MQ=255)
         gcgTCAAGGCCTGTACCACTTGCCACTGCATCGTGCGCAAGGGCTTCGATTCGCTCGAAGAGGCTGACGAGCTGGAAGAGGACATGCTGGACAAAGCCTGGGGCCTGGAAGCGCAATCGCGCCTGGGTTGCCAGGTTTTCGTCGCTGAc                                                                                                                                     >  1:13730/1‑149 (MQ=255)
            tCAAGGCCTGTACCACTTGCCACTGCATCGTGCGCAAGGGCTTCGATTCGCTCGAAGAGGCTGACGAGCTGGAAGAGGACATGCTGGACAAAGCCTGGGGCCTGGAAGCGCAATCGCGCCTGGGTTGCCAGGTTTTCGTCGCt                                                                                                                                        <  6:153926/143‑1 (MQ=255)
            tCAAGGCCTGTACCACTTGCCACTGCATCGTGCGCAAGGGCTTCGATTCGCTCGAAGAGGCTGACGAGCTGGAAGAGGACATGCTGGACAAAGCCTGGGGCCTGGAAGCGCAATCGCGCCTGGGTTGCCAGGTTTTCGTCGCt                                                                                                                                        >  5:153926/1‑143 (MQ=255)
               aGGCCTGTACCACTTGCCACTGCATCGTGCGCAAGGGCTTCGATTCGCTCGAAGAGGCTGACGAGCTGGAAGAGGACATGCTGGACAAAGCCTGGGGCCTGGAAGCGCAATCGCGCCTGGGTTGCCAGGTTTTCGTCGCTGACGAAGAc                                                                                                                               <  3:239508/149‑1 (MQ=255)
               aGGCCTGTACCACTTGCCACTGCATCGTGCGCAAGGGCTTCGATTCGCTCGAAGAGGCTGACGAGCTGGAAGAGGACATGCTGGACAAAGCCTGGGGCCTGGAAGCGCAATCGCGCCTGGGTTGCCAGGTTTTCGTCGCTGACGAAGAc                                                                                                                               <  6:192254/149‑1 (MQ=255)
                ggCCTGTACCACTTGCCACTGCATCGTGCGCAAGGGCTTCGATTCGCTCGAAGAGGCTGACGAGCTGGAAGAGGACATGCTGGACAAAGCCTGGGGCCTGGAAGCGCAATCGCGCCTGGGTTGCCAGGTCTTCGTCAGCGACGAAAAcc                                                                                                                              <  5:171500/149‑1 (MQ=255)
                 gCCTGTACCACTTGCCACTGCATCGTGCGCAAGGGCTTCGATTCGCTCGAAGAGGCTGACGAGCTGGAAGAGGACATGCTGGACAAAGCCTGGGGCCTGGAAGCGCAATCGCGCCTGGGTTGCCAGGTCTTCGTCAGCGACGAAAACCt                                                                                                                             <  4:226234/149‑1 (MQ=255)
                   cTGTACCACTTGCCACTGCATCGTGCGCAAGGGCTTCGATTCGCTCGAAGAGGCTGACGAGCTGGAAGAGGACATGCTGGACAAAGCCTGGGGCCTGGAAGCGCAATCGCGCCTGGGTTGCCAGGTTTTCGTCGCTGACGAAGACCTGa                                                                                                                           >  6:255819/1‑149 (MQ=255)
                      tACCACTTGCCACTGCATCGTGCGCAAGGGCTTCGATTCGCTCGAAGAGGCTGACGAGCTGGAAGAGGACATGCTGGACAAAGCCTGGGGCCTGGAAGCGCAATCGCGCCTGGGTTGCCAGGTTTTCGTCGCTGACGAAGACCTGACCa                                                                                                                        <  5:175540/149‑1 (MQ=255)
                         cACTTGCCACTGCATCGTGCGCAAGGGCTTCGATTCGCTCGAAGAGGCTGACGAGCTGGAAGAGGACATGCTGGACAAAGCCTGGGGCCTGGAAGCGCAATCGCGCCTGGGTTGCCAGGTTTTCGTCGCTGACGAAGACCTGACCATCg                                                                                                                     <  4:98418/149‑1 (MQ=255)
                               ccACTGCATCGTGCGCAAGGGCTTCGATTCGCTCGAAGAGGCTGACGAGCTGGAAGAGGACATGCTGGACAAAGCCTGGGGCCTGGAAGCGCAATCGCGCCTGGGTTGCCAGGTTTTCGTCGCTGACGAAGACCTGACCATCGAGATcc                                                                                                               <  5:255819/149‑1 (MQ=255)
                               ccACTGCATCGTGCGCAAGGGCTTCGATTCGCTCGAAGAGGCTGACGAGCTGGAAGAGGACATGCTGGACAAAGCCTGGGGCCTGGAAGCGCAATCGCGCCTGGGTTGCCAGGTTTTCGTCGCTGACGAAGACCTGACCATCGAGATcc                                                                                                               <  2:164461/149‑1 (MQ=255)
                                cacaGCATCGTGCGCAAGGGCTTCGATTCGCTCGAAGAGGCTGACGAGCTGGAAGAGGACATGCTGGACAAAGCCTGGGGCCTGGAAGCGCAATCGCGCCTGGGTTGCCAGGTTTTCGTCGCTGACGAAGACCTGACCATCGAGATccc                                                                                                              >  2:259072/1‑149 (MQ=255)
                                cACTGCATCGTGCGCAAGGGCTTCGATTCGCTCGAAGAGGCTGACGAGCTGGAAGAGGACATGCTGGACAAAGCCTGGGGCCTGGAAGCGCAATCGCGCCTGGGTTGCCAGGTTTtc                                                                                                                                              <  1:246936/117‑1 (MQ=255)
                                cACTGCATCGTGCGCAAGGGCTTCGATTCGCTCGAAGAGGCTGACGAGCTGGAAGAGGACATGCTGGACAAAGCCTGGGGCCTGGAAGCGCAATCGCGCCTGGGTTGCCAGGTTTtc                                                                                                                                              >  2:246936/1‑117 (MQ=255)
                                     cATCGTGCGCAAGGGCTTCGATTCGCTCGAAGAGGCTGACGAGCTGGAAGAGGACATGCTGGACAAAGCCTGGGGCCTGGAAGCGCAATCGCGCCTGGGTTGCCAGGTTTTCGTCGCTGACGAAGACCTGACCATCGAGATCCCCAAGt                                                                                                         >  4:162395/1‑149 (MQ=255)
                                       tCGTGCGCAAGGGCTTCGATTCGCTCGAAGAGGCTGACGAGCTGGAAGAGGACATGCTGGACAAAGCCTGGGGCCTGGAAGCGCAATCGCGCCTGGGTTGCCAGGTTTTCGTCGCTGACGAAGACCTGACCATCGAGATCCCCAAGTAt                                                                                                       >  6:172412/1‑149 (MQ=255)
                                         gTGCGCAAGGGCTTCGATTCGCTCGAAGAGGCTGACGAGCTGGAAGAGGACATGCTGGACAAAGCCTGGGGCCTGGAAGCGCAATCGCGCCTGGGTTGCCAGGTTTTCGTCGCTGACGAAGACCTGACCATCGAGATCCCCAAGTATTc                                                                                                     >  2:104347/1‑149 (MQ=255)
                                           gcgcAAGGGCTTCGATTCGCTCGAAGAGGCTGACGAGCTGGAAGAGGACATGCTGGACAAAGCCTGGGGCCTGGAAGCGCAATCGCGCCTGGGTTGCCAGGTTTTCGTCGCTGACGAAGACCTGACCATCGAGATCCCCAAGTATTCGc                                                                                                   >  8:210237/1‑149 (MQ=255)
                                            cgcAAGGGCTTCGATTCGCTCGAAGAGGCTGACGAGCTGGAAGAGGACATGCTGGACAAAGCCTGGGGCCTGGAAGCGCAATCACGCCTGGGTTGCCAGGTTTTCGTCGCTGACGAAGACCTGACCATCGAGATCCCCAAGTATTCGCt                                                                                                  >  3:188919/1‑149 (MQ=255)
                                                 gggCTTCGATTCGCTCGAAGAGGCTGACGAGCTGGAAGAGGACATGCTGGACAAAGCCTGGGGCCTGGAAGCGCAATCGCGCCTGGGTTGCCAGGTTTTCGTCGCTGACGAAGACCTGACCATCGAGATCCCCAAGTATTCGCTCAAcc                                                                                             <  5:172412/149‑1 (MQ=255)
                                                     ttCGATTCGCTCGAAGAGGCTGACGAGCTGGAAGAGGACATGCTGGACAAAGCCTGGGGCCTGGAAGCGCAATCGCGCCTGGGTTGCCAGGTTTTCGTCGCTGACGAAGACCTGACCATCGAGATCCCCAAGTATTCGCTCAACCAtgc                                                                                         >  4:155592/1‑149 (MQ=255)
                                                     ttCGATTCGCTCGAAGAGGCTGACGAGCTGGAAGAGGACATGCTGGACAAAGCCTGGGGCCTGGAAGCGCAATCGCGCCTGGGTTGCCAGGTTTTCGTCGCTGACGAAGACCTGACCATCGAGATCCCCAAGTATTCGCTCAACCAtgc                                                                                         >  1:213701/1‑149 (MQ=255)
                                                       cGATTCGCTCGAAGAGGCTGACGAGCTGGAAGAGGACATGCTGGACAAAGCCTGGGGCCTGGAAGCGCAATCGCGCCTGGGTTGCCAGGTTTTCGTCGCTGACGAAGACCTGACCATCGAGATCCCCAAGTATTCGCTCAACCAtgc                                                                                         <  2:33101/147‑1 (MQ=255)
                                                        gATTCGCTCGAAGAGGCTGACGAGCTGGAAGAGGACATGCTGGACAAAGCCTGGGGCCTGGAAGCGCAATCGCGCCTGGGTTGCCAGGTTTTCGTCGCTGACGAAGACCTGACCATCGAGATCCCCAAGTATTCGCTCAACCAtgctgc                                                                                      >  2:60483/1‑149 (MQ=255)
                                                               tCGAAGAGGCTGACGAGCTGGAAGAGGACATGCTGGACAAAGCCTGGGGCCTGGAAGCGCAATCGCGCCTGGGTTGCCAGGTTTTCGTCGCTGACGAAGACCTGACCATCGAGATCCCCAAGTATTCGCTCAACCATGCTGCCGAAGCa                                                                               >  2:25592/1‑149 (MQ=255)
                                                               tCGAAGAGGCTGACGAGCTGGAAGAGGACATGCTGGACAAAGCCTGGGGCCTGGAAGCGCAATCGCGCCTGGGTTGCCAGGTTTTCGTCGCTGACGAAGACCTGACCATCGAGATCCCCAAGTATTCGCTCAACCATGCTGCCGAAGCa                                                                               >  5:27762/1‑149 (MQ=255)
                                                                cGAAGAGGCGGACGAGCTGGAAGAGGACATGCTGGACAAAGCCTGGGGCCTGGAAGCGCAATCGCGCCTGGGTTGCCAGGTTTTCGTCGCTGACGAAGACCTGACCATCGAGATCCCCAAGTATTCGCTCAACCATGCTGCCGAAGCAc                                                                              <  6:132050/149‑1 (MQ=255)
                                                                  aaGAGGCTGACGAGCTGGAAGAGGACATGCTGGACAAAGCCTGGGGCCTGGAAGCGCAATCGCGCCTGGGTTGCCAGGTTTTCGTCGCTGACGAAGACCTGACCATCGAGATCCCCAAGTATTCGCTCAACCATGCTGCCGAAGCACCg                                                                            <  3:162395/149‑1 (MQ=255)
                                                                        cTGACGAGCTGGAAGAGGACATGCTGGACAAAGCCTGGGGCCTGGAAGCGCAATCGCGCCTGGGTTGCCAGGTTTTCGTCGCTGACGAAGACCTGACCATCGAGATCCCCAAGTATTCGCTCAACCATGCTGCCGAAGCACCGCACTGa                                                                      <  6:91717/149‑1 (MQ=255)
                                                                         tGACGAGCTGGAAGAGGACATGCTGGACAAAGCCTGGGGCCTGGAAGCGCAATCGCGCCTGGGTTGCCAGGTTTTCGTCGCTGACGAAGACCTGACCATCGAGATCCCCAAGTATTCGCTCAACCAtgct                                                                                        <  6:58937/130‑1 (MQ=255)
                                                                         tGACGAGCTGGAAGAGGACATGCTGGACAAAGCCTGGGGCCTGGAAGCGCAATCGCGCCTGGGTTGCCAGGTTTTCGTCGCTGACGAAGACCTGACCATCGAGATCCCCAAGTATTCGCTCAACCAtgct                                                                                        >  5:58937/1‑130 (MQ=255)
                                                                         tGACGAGCTGGAAGAGGACATGCTGGACAAAGCCTGGGGCCTGGAAGCGCAATCGCGCCTGGGTTGCCAGGTTTTCGTCGCTGACGAAGACCTGACCATCGAGATCCCCAAGTATTCGCTCAACCATGCTGCCGAAGCACCGCACTGAg                                                                     >  2:88865/1‑149 (MQ=255)
                                                                           aCGAGCTGGAAGAGGACATGCTGGACAAAGCCTGGGGCCTGGAAGCGCAATCGCGCCTGGGTTGCCAGGTTTTCGTCGCTGACGAAGACCTGACCATCGAGATCCCCGAGTATTCGCTCAACCATGCTGCCGAAGCACCGCACTGAgg                                                                    <  2:13730/148‑1 (MQ=255)
                                                                                 tGGAAGAGGACATGCTGGACAAAGCCTGGGGCCTGGAAGCGCAATCGCGCCTGGGTTGCCAGGTTTTCGTCGCTGACGAAGACCTGACCATCGAGATCCCCAAGTATTCGCTCCACCATGCTGCCGAAGCACCGCACTGAGGGCCTGTa                                                             >  8:155146/1‑149 (MQ=255)
                                                                                   gAAGAGGACATGCTGGAGAAAGCGGGGGGCCTGGAAGCGCAATGGCGCGTGGGTTGCCAGGTTTTCGTCGCTGACGATGACGTGACCATCGAGATCCCCTAGTATTCGCTCAACGATGCTGGCGAAGCTCCGCTGTGAGGGCCTGTAc                                                            <  1:255005/148‑1 (MQ=255)
                                                                                   gAAGAGGACATGCTGGACAAAGCCTGGGGCCTGGAAGCGCAATCGCGCCTGGGTTGCCAGGTTTTCGTCGCTGACGAAGACCTGACCATCGAGATCCCCAAGTATTCGCTCAACCATGCTGCCGAAGCACCGCACTGAGGGCCTGTAc                                                            <  1:259072/148‑1 (MQ=255)
                                                                                    aaGAGGACATGCTGGACAAAGCCTGGGGCCTGGAAGCGCAATCGCGCCTGGGTTGCCAGGTTTTCGTCGCTGACGAAGACCTGACCATCGAGATCCCCAAGTATTCGCTCAACCATGCTGCCGAAGCACCGCACTGAGGGCCTGTACAt                                                          <  1:88865/149‑1 (MQ=255)
                                                                                    aaGAGGACATGCTGGACAAAGCCTGGGGCCTGGAAGCGCAATCGCGCCTGGGTTGCCAGGTTTTCGTCGCTGACGAAGACCTGACCATCGAGATCCCCAAGTATTCGCTCAACCATGCTGCCGAAGCACCGCACTGAGGGCCTGTACAt                                                          >  3:70406/1‑149 (MQ=255)
                                                                                        ggACATGCTGGACAAAGCCTGGGGCCTGGAAGCGCAATCGCGCCTGGGTTGCCAGGTTTTCGTCGCTGACGAAGACCTGACCATCGAGATCCCCAAGTATTCGCTCAACCATGCTGCCGAAGCACCGCACTGAGGGCCTGTACATGAGt                                                      <  6:222320/149‑1 (MQ=255)
                                                                                             tGCTGGACAAAGCCTGGGGCCTGGAAGCGCAATCGCGCCTGGGTTGCCAGGTTTTCGTCGCTGACGAAGACCTGACCATCGAGATCCCCAAGTATTCGCTCAACCATGCTGCCGAAGCACCGCACTGAGGGCCTGTACATGAGTCTGa                                                  >  7:39734/1‑148 (MQ=255)
                                                                                             tGCTGGACAAAGCCTGGGGCCTGGAAGCGCAATCGCGCCTGGGTTGCCAGGTTTTCGTCGCTGACGAAGACCTGACCATCGAGATCCCCAAGTATTCGCTCAACCATGCTGCCGAAGCACCGCACTGAGGGCCTGTACATGAGTCTGa                                                  >  5:88643/1‑148 (MQ=255)
                                                                                              gCTGGACAAAGCCTGGGGCCTGGAAGCGCAATCGCGCCTGGGTTGCCAGGTTTTCGTCGCTGACGAAGACCTGACCATCGAGATCCCCAAGTATTCGCTCAACCATGCTGCCGAAGCACCGCACTGAGGGCCTGt                                                              >  4:96285/1‑135 (MQ=255)
                                                                                              gCTGGACAAAGCCTGGGGCCTGGAAGCGCAATCGCGCCTGGGTTGCCAGGTTTTCGTCGCTGACGAAGACCTGACCATCGAGATCCCCAAGTATTCGCTCAACCATGCTGCCGAAGCACCGCACTGAGGGCCTGt                                                              <  3:96285/135‑1 (MQ=255)
                                                                                                 tgaaaacgCCTGGGGCCTGTAAGCCCAATCGCGCGTGGGTTGTACCCCTTTCGTCGCTGACGAAGACCTGACCATCGAGATCCCCAAGTATTCGCTCAACCATGCTGCCGAAGCACCGCACTGAGGGCCTGTACATGAGTCTGAAATgg                                             <  1:224716/142‑1 (MQ=255)
                                                                                                  gACAAAGCCTGGGGCCTGGAAGCGCAATCGCGCCTGGGTTGCCAGGTTTTCGTCGCTGACGAAGACCTGACCATCGAGATCCCCAAGTATTCGCTCAACCATGCTGCCGAAGCACCGCACTGAGGGCCTGTACATGAGTCTGAAATggg                                            <  1:60483/149‑1 (MQ=255)
                                                                                                  gACAAAGCCTGGGGCCTGGAAGCGCAATCGCGCCTGGGTTGCCAGGTTTTCGTCGCTGACGAAGACCTGACCATCGAGATCCCCAAGTATTCGCTCAACCATGCTGCCGAAGCACCGCACTGAGGGCCTGTACATGAGTCTGAAATgg                                             <  6:27762/148‑1 (MQ=255)
                                                                                                   aCAAAGCCTGGGGCCTGGAAGCGCAATCGCGCCTGGGTTGCCAGGTTTTCGTCGCTGACGAAGACCTGACCATCGAGATCCCCAAGTATTCGCTCAACCATGCTGCCGAAGCACCGCACTGAGGGCCTGTACATGAGTCTGAAATGGGt                                           >  3:201910/1‑149 (MQ=255)
                                                                                                     aaaGCCTGGGGCCTGGAAGCGCAATCGCGCCTGGGTTGCCAGGTTTTCGTCGCTGACGAAGACCTGACCATCGAGATCCCCAAGTATTCGCTCAACCATGCTGCCGAAGCACCGCACTGAGGGCCTGTACATGAGTCTGAAATGGGtt                                          >  3:147405/1‑148 (MQ=255)
                                                                                                     aaaGCCTGGGGCCTGGAAGCGCAATCGCGCCTGGGTTGCCAGGTTTTCGTCGCTGACGAAGACCTGACCATCGAGATCCCCAAGTATTCGCTCAACCATGCTGCCGAAGCACCGCACTGAGGGCCTGTACATGAGTCTGAAATGGGtt                                          <  4:147405/148‑1 (MQ=255)
                                                                                                      aaGCCTGGGGCCTGGAAGCGCAATCGCGCCTGGGTTGCCAGGTTTTCGTCGCTGACGAAGACCTGACCATCGAGATCCCCAAGTATTCGCTCAACCATGCTGCCGAAGCACCGCACTGAGGGCCTGTACATGAGTCTGAAATGGGTTGa                                        <  3:155592/149‑1 (MQ=255)
                                                                                                          cTGGGGCCTGGAAGCGCAATCGCGCCTGGGTTGCCAGGTTTTCGTCGCTGACGAAGACCTGACCATCGAGATCCCCAAGTATTCGCTCAACCATGCTGCCGAAGCACCGCACTGAGGGCCTGTACATGAGTCTGAAATGGGTTGATGTa                                    <  4:188919/149‑1 (MQ=255)
                                                                                                                 cTGGAAGCGCAATCGCGCCTGGGTTGCCAGGTTTTCGTCGCTGACGAAGACCTGACCATCGAGATCCCCAAGTATTCGCTCAACCATGCTGCCGAAGCACCGCACTGAGGGCCTGTACATGAGTCTGAAATGGGTTGATGTACtt                                 >  8:167562/1‑145 (MQ=255)
                                                                                                                  tGGAAGCGCAATCGCGCCTGGGTTGCCAGGTTTTCGTCGCTGACGAAGACCTGACCATCGAGATCCCCAAGTATTCGCTCAACCATGCTGCCGAAGCACCGCACTGAg                                                                     <  3:80478/108‑1 (MQ=255)
                                                                                                                  tGGAAGCGCAATCGCGCCTGGGTTGCCAGGTTTTCGTCGCTGACGAAGACCTGACCATCGAGATCCCCAAGTATTCGCTCAACCATGCTGCCGAAGCACCGCACTGAg                                                                     >  4:80478/1‑108 (MQ=255)
                                                                                                                   ggAAGCGCAATCGCGCCTGGGTTGCCAGGTTTTCGTCGCTGACGAAGACCTGACCATCGAGATCCCCAAGTATTCGCTCAACCATGCTGCCGAAGCACCGCACTGAGGGCCTGTACATGAGTCTGAAATGGGTTGATGTACtt                                 >  7:191375/1‑143 (MQ=255)
                                                                                                                    gAAGCGCAATCGCGCCTGGGTTGCCAGGTTTTCGTCGCTGACGAAGACCTGACCATCGAGATCCCCAAGTATTCGCTCAACCATGCTGCCGAAGCACCGCACTGAGGGCCTGTACATGAGTCTGAAATGGGTTGATGTACtt                                 >  4:63725/1‑142 (MQ=255)
                                                                                                                    gAAGCGCAATCGCGCCTGGGTTGCCAGGTTTTCGTCGCTGACGAAGACCTGACCATCGAGATCCCCAAGTATTCGCTCAACCATGCTGCCGAAGCACCGCACTGAGGGCCTGTACATGAGTCTGAAATGGGTTGATGTACtt                                 <  3:63725/142‑1 (MQ=255)
                                                                                                                    gAAGCGCAATCGCGCCTGGGTTGCCAGGTTTTCGTCGCTGACGAAGACCTGACCATCGAGATCCCCAAGTATTCGCTCAACCATGCTGCCGAAGCACCGCACTGAGGGCCTGTACATGAGTCTGAAATGGGTTGATGTACTTGAGATc                           <  7:210237/148‑1 (MQ=255)
                                                                                                                         gcAATCGCGCCTGGGTTGCCAGGTTTTCGTCGCTGACGAAGACCTGACCATCGAGATCCCCAAGTATTCGCTCAACCATGCTGCCGAAGCACCGCACTGAGGGCCTGTACATGAGTCTGAAATGGGTTGATGTACTTGAGATCGCAATc                     <  2:213701/149‑1 (MQ=255)
                                                                                                                            aTCGCGCCTGGGTTGCCAGGTTTTCGTCGCTGACGAAGACCTGACCATCGAGATCCCCAAGTATTCGCTCAACCATGCTGCCGAAGCACCGCACTGAGGGCCTGTACATGa                                                        <  1:230469/111‑1 (MQ=255)
                                                                                                                            aTCGCGCCTGGGTTGCCAGGTTTTCGTCGCTGACGAAGACCTGACCATCGAGATCCCCAAGTATTCGCTCAACCATGCTGCCGAAGCACCGCACTGAGGGCCTGTACATGa                                                        >  2:230469/1‑111 (MQ=255)
                                                                                                                             tCGCGCCTGGGTTGCCAGGTTTTCGTCGCTGACGAAGACCTGACCATCGAGATCCCCAAGTATTCGCTCAACCATGCTGCCGAAGCACCGCACTGAGGGCCTGTACATGAGTCTGAAATGGGTTGATGTACTTGAGATCGCAATCCAGc                 >  8:124474/1‑149 (MQ=255)
                                                                                                                               gcgcCTGGGTTGCCAGGTTTTCGTCGCTGACGAAGACCTGACCATCGAGATCCCCAAGTATTCGCTCAACCATGCTGCCGAAGCACCGCACTGAGGGCCTGTACATGAGTCTGAAATGGGTTGATGTACTTGAGATCGCAATCCAGCt                <  8:39734/148‑1 (MQ=255)
                                                                                                                                  ccTGGGTTGCCAGGTTTTCGTCGCTGACGAAGACCTGACCATCGAGATCCCCAAGTATTCGCTCAACCATGCTGCCGAAGCACCGCACTGAGGGCCTGTACATGAGTCTGAAATGGGTTGATGTACTTGAGATCGCAATCCAGCTTGCa            <  6:88643/149‑1 (MQ=255)
                                                                                                                                      ggTTGCCAGGTTTTCGTCGCTGACGAAGACCTGACCATCGAGATCCCCAAGTATTCGCTCAACCATGCTGCCGAAGCACCGCACTGAGGGCCTGTACATGAGTCTGAAATGGGTTGATGTACTTGAGATCGCAATCCAGCTTGCAGaaa        <  4:201910/149‑1 (MQ=255)
                                                                                                                                        tggtcAGGTCTTCGTCAGCGACGAAAACCTGACCATCGAGATCCCCAAGTATTCGCTCAACCATGCTGCCGAAGCACCGCACTGAGGGCCTGTACATGAGTCTGAAATGGGTTGATGTACTTGAGATCGCAATCCAGCTTGCAGAaag       >  2:230138/5‑148 (MQ=255)
                                                                                                                                         ggtcAGGTCTTCGTCAGCGACGACAACCTGACCATCGAGATCCCCCAGTAGTCGCTCACCCATGCTGCCGACGCACCGCACTGAGGGCCTGTACAGGAGTCTGAAATGGGTTGATGTACTTGAGATCGCAATCCAGCTTGCAGAaagc      >  5:164000/4‑148 (MQ=255)
                                                                                                                                          gCCAGGTTTTCGTCGCTGACGAAGACCTGACCATCGAGATCCCCAAGTATTCGCTCAACCATGCTGCCGAAGCACCGCACTGAGGGCCTGTACATGAGTCTGAAATGGGTTGATGTACTTGAGATCGCAATCCAGCTTGCAGAaagca     <  4:70406/148‑1 (MQ=255)
                                                                                                                                           tcAGGTCTTCGTCAGCGACGAAAACCTGACCATCGAGATCCCCAAGTATTCGCTCAACCATGCTGCCGAAGCACCGCACTGAGGGCCTGTACATGAGTCTGAAATGGGTTGATGTACTTGAGATCGCAATCCAGCTTGCAGAaagcaag   >  2:150053/2‑149 (MQ=255)
                                                                                                                                            cAGGTTTTCGTCGCTGACGAAGACCTGACCATCGAGATCCCCAAGTATTCGCTCAACCATGCTGCCGAAGCACCGCACTGAGGGCCTGTACATGAGTCTGAAATgg                                             <  7:70055/106‑1 (MQ=255)
                                                                                                                                            cAGGTTTTCGTCGCTGACGAAGACCTGACCATCGAGATCCCCAAGTATTCGCTCAACCATGCTGCCGAAGCACCGCACTGAGGGCCTGTACATGAGTCTGAAATgg                                             >  8:70055/1‑106 (MQ=255)
                                                                                                                                            cAGGTTTTCGTCGCTGACGAAGACCTGACCATCGAGATCCCCAAGTATTCGCTCAACCATGCTGCCGAAGCACCGCACTGAGGGCCTGTACATGAGTCTGAAATGGGTTGATGTACTTGAGATCGCAATCCAGCTTGCAGAaagcaagc  <  3:8790/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
CCTGCGGCGGCGTCAAGGCCTGTACCACTTGCCACTGCATCGTGCGCAAGGGCTTCGATTCGCTCGAAGAGGCTGACGAGCTGGAAGAGGACATGCTGGACAAAGCCTGGGGCCTGGAAGCGCAATCGCGCCTGGGTTGCCAGGTTTTCGTCGCTGACGAAGACCTGACCATCGAGATCCCCAAGTATTCGCTCAACCATGCTGCCGAAGCACCGCACTGAGGGCCTGTACATGAGTCTGAAATGGGTTGATGTACTTGAGATCGCAATCCAGCTTGCAGAAAGCAAGC  >  NC_002947/983111‑983399

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑