Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,042,285 G→A 8.1% G32D (GGC→GAC)  colS → integral membrane sensor signal transduction histidine kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,042,2850GA 8.1% 137.0 / 2.5 49G32D (GGC→GAC) colSintegral membrane sensor signal transduction histidine kinase
Reads supporting (aligned to +/- strand):  ref base G (21/24);  new base A (2/2);  total (23/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.42e-01

CCGTTGCTGCATACCGTCCATGGCGTCGGCTATCGCCTCGCCGAGGGCCGCGATGGAGTTTAAGCAAAGCCTTGCCCAGCGCATCATCATCGCCTTTGCCCTGATGAGCGCGCTGGTGGCCGGGGCCTTCGCATTCGGTATCGTCGGCACGGTGCACCTGGTCGAAGAGCGACTGATTTCCTCGGTACTGGGCGGTGACCTGCAGCGCCTGCTGCGTATGGACAGCGTCAGTGACTGGAGTCATCGGCCTCGTCCAGACCAGCTGTTCTATTTCAGTGGCGGGCGTGAC  >  NC_002947/1042139‑1042427
                                                                                                                                                  |                                                                                                                                              
ccGTTGCTGCATACCGTCCATGGCGTCGGCTATCGCCTCGCCGAGGGCCGCGATGGAGTTTAAGCAAAGCCTTGCCCAGCGCATCATCATCGCCTTTGCCCTGATGAGCGCGCTGGTGGCCGGGGCCTTCGCATTCGGTATCGTCGGCa                                                                                                                                              >  3:86886/1‑149 (MQ=255)
             ccGTCCATGGCGTCGGCTATCGCCTCGCCGAGGGCCGCGATGGAGTTTAAGCAAAGCCTTGCCCAGCGCATCATCATCGCCTTTGCCCTGATGAGCGCGCTGGTGGCCGGGGCCTTCGCATTCGGTATCGTCGGCACGGTGCACCTgg                                                                                                                                  <  1:65706/148‑1 (MQ=255)
                  cATGGCGTCGGCTATCGCCTCGCCGAGGGCCGCGATGGAGTTTAAGCAAAGCCTTGCCCAGCGCATCATCATCGCCTTTGCCCTGATGAGCGCGCTGGTGGCCGGGGCCTTCGCATTCGGTATCGTCGGCACGGTGCACCTGGTCGaa                                                                                                                             <  4:86886/148‑1 (MQ=255)
                      gCGTCGGCTATCGCCTCGCCGAGGGCCGCGATGGAGTTTAAGCAAAGCCTTGCCCAGCGCATCATCATCGCCTTTGCCCTGATGAGCGCGCTGGTGGCCGGGGCCTTCGCATTCGGTATCGTCGGCACGGTGCACCTGGTCGAAGAGc                                                                                                                         >  1:187647/1‑148 (MQ=255)
                       cGTCGGCTATCGCCTCGCCGAGGGCCGCGATGGAGTTTAAGCAAAGCCTTGCCCAGCGCATCATCATCGCCTTTGCCCTGATGAGCGCGCTGGTGGCCGGGGCCTTCGCATTCGGTATCGTCGGCACGGTGCACCTGGTCGAAGAGCGa                                                                                                                       <  4:208900/149‑1 (MQ=255)
                         tCGGCTATCGCCTCGCCGAGGGCCGCGATGGAGTTTAAGCAAAGCCTTGCCCAGCGCATCATCATCGCCTTTGCCCTGATGAGCGCGCTGGTGGCCGGGGCCTTCGCATTCGGTATCGTCGGCACGGTGCACCTGGTCGAAGAGCGACt                                                                                                                     >  8:2160/1‑149 (MQ=255)
                             cTATCGCCTCGCCGAGGGCCGCGATGGAGTTTAAGCAAAGCCTTGCCCAGCGCATCATCATCGCCTTTGCCCTGATGAGCGCGCTGGTGGCCGGGGCCTTCGCATTCGGTATCGTCGGCACGGTGCACCTGGTCGAAGAGCGACTGAtt                                                                                                                 <  7:2160/149‑1 (MQ=255)
                                  gcctcgccGAGGGCCGCGATGGAGTTTAAGCAAAGCCTTGCCCAGCGCATCATCATCGCCTTTGCCCTGATGAGCGCGCTGGTGGCCGGGGCCTTCGCATTCGGTATCGTCGGCACGGTGCACCTGGTCGAAGAGCGACTGATTTCCt                                                                                                             <  1:38523/148‑1 (MQ=255)
                                    ctcgccGAGGGCCGCGATGGAGTTTAAGCAAAGCCTTGCCCAGCGCATCATCATCGCCTTTGCCCTGATGAGCGCGCTGGTGGCCGGGGCCTTCGCATTCGGTATCGTCGGCACGGTGCACCTGGTCGAAGAGCGACTGATTTCCTCgg                                                                                                          <  2:177074/149‑1 (MQ=255)
                                               ccGCGATGGAGTTTAAGCAAAGCCTTGCCCAGCGCATCATCATCGCCTTTGCCCTGATGAGCGCGCTGGTGGCCGGGGCCTTAGCATTCGGTATCGTCGGCACGGTGCACCTGCTCGAAGAGCGACTGATTTGCTCGGTAATGGGCGGt                                                                                               >  8:100073/1‑149 (MQ=255)
                                                   gATGGAGTTTAAGCAAAGCCTTGCCCAGCGCATCATCATCGCCTTTGCCCTGATGAGCGCGCTGGTGGCCGGGGCCTTCGCATTCGGTATCGTCGGCACGGTGCACCTGGTCGAaga                                                                                                                           <  6:146862/117‑1 (MQ=255)
                                                   gATGGAGTTTAAGCAAAGCCTTGCCCAGCGCATCATCATCGCCTTTGCCCTGATGAGCGCGCTGGTGGCCGGGGCCTTCGCATTCGGTATCGTCGGCACGGTGCACCTGGTCGAaga                                                                                                                           >  5:146862/1‑117 (MQ=255)
                                                          tttAAGCAAAGCCTTGCCCAGCGCATCATCATCGCCTTTGCCCTGATGAGCGCGCTGGTGGCCGGGGCCTTCGCATTCGGTATCGTCGGCCCGGTGCACCTGGTCGAAGGGCGCCTGCTTTCGGCGGTACTGGGCGGTGGCCTGCAgcg                                                                                    >  8:235171/1‑149 (MQ=255)
                                                           ttAAGCAAAGCCTTGCCCAGCGCATCATCATCGCCTATGCCCTGATGAGCGCGCTGGTGGCCGGGGCCTTCGCATTCGGTATCGTCGGCACGGTGCACCTGGTCGAAGAGCGACTGATTTCCTCGGTACTGGGCGGTGACCTGCAgcgc                                                                                   >  1:44862/1‑149 (MQ=255)
                                                               gCAAAGCCTTGCGCAGCGCATCATCATCGCCTTTGCGCTGATGAGCGCGCTGGTGGCCGGGGCCTTGGCTTTCGGTTTCGTCGGCACGGTGCACCTGGTCTAagag                                                                                                                          <  1:43259/106‑1 (MQ=255)
                                                               gCAAAGCCTTGCCCAGCGCATCATCATCGCCTTTGCCCTGATGAGCGCGCTGGTGGCCGGGGCCTTCGCATTCGGTATCGTCGGCTCGGTGCACCTGGTCGAAGAGCGACTGATTTCCTCGGTACTGGGCGGt                                                                                               <  6:113238/133‑1 (MQ=255)
                                                               gCAAAGCCTTGCCCAGCGCATCATCATCGCCTTTGCCCTGATGAGCGCGCTGGTGGCCGGGGCCTTCGCATTCGGTATCGTCGGCCCGGTGCACCTGGTCGAAGAGCGACTGATTTCCTCGGTACTGGGCGGt                                                                                               >  5:113238/1‑133 (MQ=255)
                                                               gCAAACCCTTGCCCAGCGCATCATCATCGCCTTTGCCATGATGAGCGCGCTGGTGTCCTGGGCCTTCGCATTCGGTATCGTCGGCACGATGAACCTGGTCGAacag                                                                                                                          >  2:43259/1‑106 (MQ=255)
                                                                       ttGCCCAGCGCATCATCATCGCCTTTGCCCTGATGAGCGCGCTGGTGGCCGGGGCCTTCGCATTCGGTATCGTCGACACGGTGCACCTGGTCGAAGAGCGACTGATTTCCTCGGTACTg                                                                                                     <  5:112043/119‑1 (MQ=255)
                                                                       ttGCCCAGCGCATCATCATCGCCTTTGCCCTGATGAGCGCGCTGGTGGCCGGGGCCTTCGCATTCGGTATCGTCGACACGGTGCACCTGGTCGAAGAGCGACTGATTTCCTCGGTACTg                                                                                                     >  6:107815/1‑119 (MQ=255)
                                                                       ttGCCCAGCGCATCATCATCGCCTTTGCCCTGATGAGCGCGCTGGTGGCCGGGGCCTTCGCATTCGGTATCGTCGACACGGTGCACCTGGTCGAAGAGCGACTGATTTCCTCGGTACTg                                                                                                     <  5:107815/119‑1 (MQ=255)
                                                                       ttGCCCAGCGCATCATCATCGCCTTTGCCCTGATGAGCGCGCTGGTGGCCGGGGCCTTCGCATTCGGTATCGTCGACACGGTGCACCTGGTCGAAGAGCGACTGATTTCCTCGGTACTg                                                                                                     >  6:112043/1‑119 (MQ=255)
                                                                         gCCCAGCGCATCATCATCGCCTTTGCCCTGATGAGCGCGCTGGTGGCCGGGGCCTTCGCATTCGGTATCGTCGGCACGGTGCACCTg                                                                                                                                   <  4:85122/87‑1 (MQ=255)
                                                                           ccAGCGCATCATCATCGCCTTTGCCCTGATGAGCGCGCTGGTGGCCGGGGCCTTCGCATTCGGTATCGTCGGCACGGTGCACCTGGTCGAAGAGCGACTGATTTCCTCGGTACTGGGCGGTGACCTGCAGCGCCTGCTGCGTATTGACa                                                                   <  2:44862/149‑1 (MQ=255)
                                                                             aGCGCATCATCATCGCCTTTGCCCTGCTGAGCGCGCTGGTGGCCGGGGCCTTCGCATTCGGTATCGTCGGCACGGTGCACCTGGTCGAAGAGCGACTGATTTCCTCGGTACTGGGCGGTGACCTGCAGCGCCTGCTGCGTATGGACAGc                                                                 <  7:235171/149‑1 (MQ=255)
                                                                                  atcatcatcGCCTTTGCCCTGATGAGCGCGCTGGTGGCCGGGGCCTTCGCATTCGGTATCGTCGGCACGGTGCACCTGGTCGAAGAGCGACTGATTTCCTCGGTACTGGGCGGTGACCTGCAGCGcc                                                                                  >  2:232935/1‑127 (MQ=255)
                                                                                   tcatcatcGCCTTTGCCCTGATGAGCGCGCTGGTGGCCGGGGCCTTCGCATTCGGTATCGTCGGCACGGTGCACCTGGTCGAAGAGCGACTGATTTCCTCGGTACTGGGCGGTGACCTGCAGCGCCTGCTGCGTATGGACAGCGTCAGt                                                           <  1:12597/149‑1 (MQ=255)
                                                                                        atcGCCTTTGCCCTGATGAGCGCGCTGGTGGCCGGGGCCTTCGCATTCGGTATCGTCGGCACGGTGCACCTGGTCGAAGAGCGACTGATTTCCTCGGTACTGGGCGGTGACCTGCAGCGCCTGCTGCGTATGGACAGCGTCAGTGACt                                                       <  1:107820/148‑1 (MQ=255)
                                                                                        atcGCCTTTGCCCTGATGAGCGCGCTGGTGGCCGGGGCCTTCGCATTCGGTATCGTCGGCACGGTGCACCTGGTCGAAGAGCGACTGATTTCCTCGGTACTGGGCGGTGACCTGCAGCGCCTGCTGCGTATGGACAGCGTCAGTGACTg                                                      >  1:197785/1‑149 (MQ=255)
                                                                                         tcGCCTTTGCCCTGATGAGCGCGCTGGTGGCCGGGGCCTTCGCATTCGGTATCGTCGGCACGGTGCACCTGGTCGAAGAGCGACTGATTTCCTCGGTACTGGGCGGTGACCTGCAGCGCCTGCTGCGTATGGACAGCGTCAGTGACTgg                                                     <  3:66409/149‑1 (MQ=255)
                                                                                         tcGCCTTTGCCCTGATGAGCGCGCTGGTGGCCGGGGCCTTCGCATTCGGTATCGTCGGCACGGTGCACCTGGTCGAAGAGCGACTGATTTCCTCGGTACTGGGCGGTGACCTGCAGCGCCTGCTGCGTATGGACAGCGTCAGTGACTg                                                      >  1:91530/1‑148 (MQ=255)
                                                                                            ccTTTGCCCTGATGAGCGCGCTGGTGGCCGGGGCCTTCGCATTCGGTATCGTCGGCACGGTGCACCTGGTCGAAGAGCGACTGATTTCCTCGGTACTGGGCGGTGACCTGCAGCGCCTGCTGCGTATGGACAGCGTCAGTGACTGGAg                                                   >  5:69703/1‑148 (MQ=255)
                                                                                             cTTTGCCCTGATGAGCGCGCTGGTGGCCGGGGCCTTCGCATTCGGTATCGTCGGCACGGTGCACCTGGTCGAAGAGCGACTGATTTCCTCGGTACTGGGCGGTGACCTGCAGCGCCTGCTGCGTATGGACAGCGTCAGTGACTGGAGt                                                  >  7:17021/1‑148 (MQ=255)
                                                                                              tttGCCCTGATGAGCGCGCTGGTGGCCGGGGCCTTCGCATTCGGTATCGTCGGCACGGTGCACCTGGTCGAAGAGCGACTGATTTCCTCGGTACTGGGCGGTGACCTGCAGCGCCTGCTGCGTATGGACAGCGTCAGTGACTGGAGTCa                                                >  1:117673/1‑149 (MQ=255)
                                                                                                  cccTGATGAGCGCGCTGGTGGCCGGGGCCTTCGCATTCGGTATCGTCGGCACGGTGCACCTGGTCGAAGAGCGACTGATTTCCTCGGTACTGGGCGGTTACCTGCAGCGCCTGCTGCGTATGGACAGCGTCAGTGACTGGAGTCATCg                                             <  1:266679/148‑1 (MQ=255)
                                                                                                    cTGATGAGCGCGCTGGTGGCCGGGGCCTTCGCATTCGGTATCGTCGGCACGGTGCACCTGGTCGAAGAGCGACTGATTTCCTCGGTACTGGGCGGTGACCTGCAGCGCCTGCTGCGTATGGACAGCGTCAGTGACTGGAGTCATCGGcc                                          >  7:157420/1‑149 (MQ=255)
                                                                                                     tgatgaGCGCGCTGGTGGCCGGGGCCTTCGCATTCGGTATCGTCGGCACGGTGCACCTGGTCGAAGAGCGACTGATTTCCTCGGTACTGGGCGGTGACCTGCAGCGCCTGCTGCGTATGGACAGCGTCAGTGACTGGAGTCATCGGCCt                                         >  3:14159/1‑149 (MQ=255)
                                                                                                            cgcgcTGGTGGCCGGGGCCTTCGCATTCGGTATCGTCGGCACGGTGCACCTGGTCGAAGAGCGACTGATTTCCTCGGTACTGGGCGGTGACCTGCAGCGCCTGCTGCGTATGGACAGCGTCAGTGACTGGAGTCATCGGCCTCGTCCa                                   <  8:17021/148‑1 (MQ=255)
                                                                                                               gcTGGTGGCCGGGGCCTTCGCATTCGGTATCGTCGGCACGGTGCACCTGGTCGAAGAGCGACTGATTTCCTCGGTACTGGGCGGTGACCTGCAGCGCCTGCTGCGTATGGACAGCGTCAGTGACTGGAGTCATCGGCCTCGTCCAGa                                 <  5:17896/147‑1 (MQ=255)
                                                                                                               gcTGGTGGCCGGGGCCTTCGCATTCGGTATCGTCGGCACGGTGCACCTGGTCGAAGAGCGACTGATTTCCTCGGTACTGGGCGGTGACCTGCAGCGCCTGCTGCGTATGGACAGCGTCAGTGACTGGAGTCATCGGCCTCGTCCAGa                                 >  6:17896/1‑147 (MQ=255)
                                                                                                                      gCCGGGGCCTTCGCATTCGGTATCGTCGGCACGGTGCACCTGGTCGAAGAGCGACTGATTTCCTCGGTACTGGGCGGTGACCTGCAGCGCCTGCTGCGTATGGACAGCGTCAGTGACTGGAGTCATCGGCCTCGTCCAGACCAGCTGtt                        <  2:187647/149‑1 (MQ=255)
                                                                                                                       ccGGGGCCTTCGCATTCGGTATCGTCGGCACGGTGCACCTTGTCGAAGTGCGACTGATTTCCTCGGTACTGGGCGGTGAGCTGCAGCGCCTGCTGCGTATGGACAGCGTCAGTGACTGGAGTCATCGGCCTCGTCCAGACCAGCTGTTc                       <  7:100073/149‑1 (MQ=255)
                                                                                                                         ggggCCTTCGCATTCGGTATCGTCGGCACGGTGCACCTGGTCGAAGAGCGACTGATTTCCTCGGTACTGGGCGGTGACCTGCAGCGCCTGCTGCGTATGGACAGCGTCAGTGACTGGAGTCATCGGCCTCGTCCAGACCAGCTGTTCTa                     <  6:69703/149‑1 (MQ=255)
                                                                                                                         ggggCCTTCGCATTCGGTATCGTCGGCACGGTGCACCTGGTCGAAGAGCGACTGATTTCCTCGGTACTGGGCGGTGACCTGCAGCGCCTGCTGCGTATGGACAGCGTCAGTGACTGGAGTCATCGGCCTCGTCCAGACCAGCTGTTCTa                     <  2:117673/149‑1 (MQ=255)
                                                                                                                          gggCCTTCGCATTCGGTATCGTCGGCACGGTGCACCTGGTCGAAGAGCGACTGATTTCCTCGGTACTGGGCGGTGACCTGCAGCGCCTGCTGCGTATGGACAGCGTCAGTGACTGGAGTCATCGGCCTCGTCCAGACCAGCTGTTCTAt                    <  2:91530/149‑1 (MQ=255)
                                                                                                                                    aTTCGGTATCGTCGGCACGGTGCACCTGGTCGAAGAGCGACTGATTTCCTCGGTACTGGGCGGTGACCTGCAGCGCCTGCTGCGTATGGACAGCGTCAGTGACTGGAGTCAACCGCCTCGTCCAGCCCAGCTGTTCTAATTCAGTggcg          >  8:60498/1‑149 (MQ=255)
                                                                                                                                        ggTATCGTCGGCACGGTGCACCTGGTCGAAGAGCGACTGATTTCCTCGGTACTGGGCGGTGACCTGCAGCGCCTGCTGCGTATGGACAGCGTCAGTGACTGGAGTCATCGGCCTCGTCCAGACCAGCTGTTCTATTTCAGTggcgggcg      >  6:228986/1‑149 (MQ=255)
                                                                                                                                            tcgtcgGCACGGTGCACCTGGTCGAAGAGCGACTGATTTCCTCGGTACTGGGCGGTGACCTGCAGCGCCTGCTGCGTATGGACAGCGTCAGTGACTGGAGTCATCGGCCTCGTCCAGACCAGCTGTTCTATTTCAGTGGCGGGCGTgac  >  6:49686/1‑149 (MQ=255)
                                                                                                                                            tcgtcgGCACGGTGCACCTGGTCGAAGAGCGACTGATTTCCTCGGTACTGGGCGGTGACCTGCAGCGCCTGCTGCGTATGGACAGCGTCAGTGACTGGAGTCATCGGCCTCGTCCAGACCAGCTGTTCTATTTCAGTGGCGGGCGTga   <  4:14159/148‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                              
CCGTTGCTGCATACCGTCCATGGCGTCGGCTATCGCCTCGCCGAGGGCCGCGATGGAGTTTAAGCAAAGCCTTGCCCAGCGCATCATCATCGCCTTTGCCCTGATGAGCGCGCTGGTGGCCGGGGCCTTCGCATTCGGTATCGTCGGCACGGTGCACCTGGTCGAAGAGCGACTGATTTCCTCGGTACTGGGCGGTGACCTGCAGCGCCTGCTGCGTATGGACAGCGTCAGTGACTGGAGTCATCGGCCTCGTCCAGACCAGCTGTTCTATTTCAGTGGCGGGCGTGAC  >  NC_002947/1042139‑1042427

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑