Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,054,810 T→C 8.8% Y341C (TAC→TGC) ‡ PP_0913 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,054,8100TC 8.8% 123.3 / 2.3 45Y341C (TAC→TGC) ‡PP_0913hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (18/23);  new base C (2/2);  total (20/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTTTGAGGGCGTGGTCTACGATCACCTGAAGCTTGGCCTCGGTGGCTTCCAGATCGGCCTTGAGGGCTGCATCGGCGGCCGGGTCAACCTTGGCGACCAGCGACGACAGGCTAGGGCCGGTAAGCTTGGTGCCGTCCGGGCGGGTGTACTCGCCCAGGTAGACGTTGCGGATGCCCTTGGCGTCGTAGAAGTGCGAGTAGTGAGTGTTGTCGCTGAAGCAGTCTTGCTCGTCTTCCGGCGAGTTGGCTTCCAGCGAAACTTTCATGCGCTCGCCAGCCAGTTCGCCCAGCGA  >  NC_002947/1054664‑1054955
                                                                                                                                                  |                                                                                                                                                 
cTTTGAGGGCGTGGTCTACGATCACCTGAAGCTTGGCCTCGGTGGCTTCCAGATCGGCCTTGAGGGCTGCATCGGCGGCCGGGTCAACCTTGGCGACCAGCGACGACAGGCTAGGGCCGGTAAGCTTGGTGCCGTCCGGGCGGGTGTAc                                                                                                                                                 >  8:24088/1‑149 (MQ=255)
      gggCGTGGTCTACGATCACCTGAAGCTTGGCCTCGGTGGCTTCCAGATCGGCCTTGAGGGCTGCATCGGCGGCCGGGTCAACCTTGGCGACCAGCGACGACAGGCTAGGGCCGGTAAGCTTGGTGCCGTCCGGGCGGGTGTACTCGccc                                                                                                                                           <  2:202621/149‑1 (MQ=255)
       ggCGTGGTCTACGATCACCTGAAGCTTGGCCTCGGTGGCTTCCAGATCGGCCTTGAGGGCTGCATCGGCGGCCGGGTCAACCTTGGCGACCAGCGACGACAGGCTAGGGCCGGTAAGCTTGGTGCCGTCCGGGCGGGTGTACTCGcc                                                                                                                                            >  2:260028/1‑147 (MQ=255)
         cGTGGTCTACGATCACCTGAAGCTTGGCCTCGGTGGCTTCCAGATCGGCCTTGAGGGCTGCATCGGCGGCCGGGTCAACCTTGGCGACCAGCGACGACAGGCTAGGGCCGGTAAGCTTGGTGCCGTCCGGGCGGGTGTACTCGCCCAgg                                                                                                                                        <  5:245409/149‑1 (MQ=255)
          gTGGTCTACGATCACCTGAAGCTTGGCCTCGGTCGCTTCCAGCTCGGCCTTGAGGGCTGCATCGGCGGCCGGGTCAACCTTGGCGACCAGCGACGACAGGCTAGGGCCGGTAAGCTTGGTGCCGTCCGGGCGAGTACACCCGcccggac                                                                                                                                       <  6:92137/149‑5 (MQ=255)
               cTACGATCACCTGAAGCTTGGCCTCGGTGGCTTCCAGATCGGCCTTGAGGGCTGCATCGGCGGCCGGGTCAACCTTGGCGACCAGCGACGACAGGCTAGGGCCGGTAAGCTTGGTGCCGTCCGGGCGGGTGTACTCGCCCAGGTAGACg                                                                                                                                  >  6:176394/1‑149 (MQ=255)
                   gATCACCTGAAGCTTGGCCTCGGTGGCTTCCAGATCGGCCTTGAGGGCTGCATCGGCGGCCGGGTCAACCTTGGCGACCAGCGACGACAGGCTAGGGCCGGTAAGCTTGGTGCCGTCCGGGCGGGTGTACTCGCCCAGGTAGACGTTGc                                                                                                                              >  4:62739/1‑149 (MQ=255)
                    aTCACCTGAAGCTTGGCCTCGGTGGCTTCCAGATCGGCCTTGAGGGCTGCATCGGCGGCCGGGTCAACCTTGGCGACCAGCGACGACAGGCTAGGGCCGGTAAGCTTGGTGCCGTCCGGGGGGGTGTACTCGCCCAGGTAGACGTTGCg                                                                                                                             <  8:157157/149‑1 (MQ=255)
                     tCACCTGAAGCTTGGCCTCGGTGGCTTCCAGATCGGCCTTGAGGGCTGCATCGGCGGCCGGGTCAACCTTGGCGACCAGCGACGACAGGCTAGGGCCGGTAAGCTTGGTGCCGTCCGGGCGGGTGTACTCGCCCAGGTAGACGTTGCg                                                                                                                             >  7:177649/1‑148 (MQ=255)
                              gCTTGGCCTCGGTGGCTTCCAGATCGGCCTTGAGGGCTGCATCGGCGGCCGGGTCAACCTTGGCGACCAGCGACGACAGGCTAGGGCCGGTAAGCTTGGTGCCGTCCGGGCGGGTGTACTCGCCCAGGTAGACGTTGCGGATGCCCTTg                                                                                                                   <  5:176394/149‑1 (MQ=255)
                                             cTTCCAGATCGGCCTTGAGGGCTGCATCGGCGGCCGGGTCAACCTTGGCGACCAGCGACGACAGGCTAGGGCCGGTAAGCTTGGTGCCGTCCGGGCGGGTGTACTCGCCCAGGTAGACGTTGCGGATGCCCTTGGCGTCGTAGAAGTGc                                                                                                    >  4:87661/1‑149 (MQ=255)
                                                   gATCGGCCTTGAGGGCTGCATCGGCGGCCGGGTCAACCTTGGCGACCAGCGACGACAGGCTAGGGCCGGTAAGCTTGGTGCCGTCCGGGCGGGTGTACTCGCCCAGGTAGACGTTGCGGATGCCCTTGGCGTCGTAGAAGTGCGagtag                                                                                              <  3:87661/149‑1 (MQ=255)
                                                    aTCGGCCTTGAGGGCTGCATCGGCGGCCGGGTCAACCTTGGCGACCAGCGACGACAGGCTAGGGCCGGTAAGCTTGGTGCCGTCCGGGCGGGTGTACTCGCCCAGGTAGACGTTGCGGATGCCCTTGGCGTCGTAGAAGTGCGagtagt                                                                                             <  7:24088/149‑1 (MQ=255)
                                                     tCGGCCTTGAGGGCTGCATCGGCGGCCGGGTCAACCTTGGCGACCAGCGACGACAGGCTAGGGCCGGTAAGCTTGGTGCCGTCCGGGCGGGTGTACTCGCCCAGGTAGACGTTGCGGATGCCCTTGGCGTCGTAGAAGTGCGagtagt                                                                                             <  7:183301/148‑1 (MQ=255)
                                                       ggCCTTGAGGGCTGCATCGGCGGCCGGGTCAACCTTGGCGACCAGCGACGACAGGCTAGGGCCGGTAAGCTTGGTGCCGTCCGGGCGGGTGTACTCGCCCAGGTAGACGTTGCGGATGCCCTTGGCGTCGTAGAAGTGCGAGTagtga                                                                                           <  3:62739/148‑1 (MQ=255)
                                                                ggCTGCATCGGCGGCCGGGTCAACCTTGGCGACCAGCGACGACAGGCTAGGGCCGGTAAGCTTGGTGCCGTCCGGGCGGGTGTACTCGCCCAGGTAGACGTTGCGGATGCCCTTGGCGTCGTAGAAGTGCGAGTagtga                                                                                           <  7:49095/139‑1 (MQ=255)
                                                                ggCTGCATCGGCGGCCGGGTCAACCTTGGCGACCAGCGACGACAGGCTAGGGCCGGTAAGCTTGGTGCCGTCCGGGCGGGTGTACTCGCCCAGGTAGACGTTGCGGATGCCCTTGGCGTCGTAGAAGTGCGAGTagtga                                                                                           >  8:49095/1‑139 (MQ=255)
                                                                    gCATCGGCGGCCGGGTCAACCTTGGCGACCAGCGACGACAGGCTAGGGCCGGTAAGCTTGGTGCCGTCCGGGCGGGTGTACTCGCCCAGGTAGACGTTGCGGATGCCCTTGGCGTCGTAGAAGTGCGAGTAGTGAGTGTTGTCGCTGaa                                                                             <  3:216980/149‑1 (MQ=255)
                                                                     gaTCGGCTGCCGTGGAAACCTTCGCCACCAGCGACGACACGCTAGGGCCGGTAAGCTTGGTGCCGTCCGGGCGTGTGTACTCGCCCAGGTAGACGTTGCGTATGCCGTTGGCGTCGTAGAAGTTCGAGTAGTCAGTGTTTGCGCTGAAg                                                                            <  2:150772/148‑1 (MQ=255)
                                                                            ggcCGGGTCAACCTTGGCGACCAGCGACGACAGGCTAGGGCCGGTAAGCTTGGTGCCGTCCGGGCGGGTGTACTCGCCCAGGTAGACGTTGCGGATGCCCTTGGCGTCGTAGAAGTGCGAGTAGTGAGTGTTGTCGCTGAAGCAGTCtt                                                                     <  8:177649/149‑1 (MQ=255)
                                                                               cGGGTCAACCTTGGCGACCAGCGACGACAGGCTAGGGCCGGTAAGCTTGGTGCCGTCCGGGCGGGTGTACTCGCCCAGGTAGACGTTGCGGATGCCCTTGGCGTCGTAGAAGTGCGAGTAGTGAGTGTTGTCGCTGAAGCAGTCTTGc                                                                   >  6:66995/1‑148 (MQ=255)
                                                                                gggTCAACCTTGGCGACCAGCGACGACAGGCTAGGGCCGGTAAGCTTGGTGCCGTCCGGGCGGGTGTACTCGCCCAGGTAGACGTTGCGGATGCCCTTGGCGTCGTAGAAGTGCGAGTAGTGAGTGTTGTCGCTGAAGCAGTCTTGCTc                                                                 <  1:260028/149‑1 (MQ=255)
                                                                                  gTCAACCTTGGCGACCAGCGACGACAGGCTAGGGCCGGTAAGCTTGGTGCCGTCCGGGCGGGTGTACTCGCCCAGGTAGACGTTGCGGATGCCCTTGGCGTCGTAGAAGTGCGAGTAGTGAGTGTTGTCGCTGAAGCAGTCTTGCTCGt                                                               <  5:66995/149‑1 (MQ=255)
                                                                                   tCAACCTTGGCGACCAGCGACGACAGGCTAGGGCCGGTAAGCTTGGTGCCGTCCGGGCGGGTGTACTCGCCCAGGTAGACGTTGCGGATGCCCTTGGCGTCGTAGAAGTGCGAGTAGTGAGTGTTGTCGCTGAAGCAGTCTTGCTCGTc                                                              >  5:56363/1‑149 (MQ=255)
                                                                                     aaCCTTGGCGACCAGCGACGACAGGCTAGGGCCGGTAAGCTTGGTGCCGTCCGGGCGGGTGTACTCGCCCAGGTAGACGTTGCGGATGCCCTTGGCGTCGTAGAAGTGCGAGTAGTGAGTGTTGTCGCTGAAGCAGTCTTGCTCGTCtt                                                            >  7:31999/1‑149 (MQ=255)
                                                                                     aaCCTTGGCGACCAGCGACGACAGGCTAGGGCCGGTAAGCTTGGTGCCGTCCGGGCGGGTGTACTCGCCCAGGTAGACGTTGCGGATGCCCTTGGCGTCGTAGAAGTGCGAGTAGTGAGTGTTGTCGCTGAAGCAGTCTTGCTCGTCtt                                                            >  3:30620/1‑149 (MQ=255)
                                                                                       ccTTGGCGACCAGCGACGACAGGCTAGGGCCGGTAAGCTTGGTGCCGTCCGGGCGGGTGTACTCGCCCAGGTAGACGTTGCGGATGCCCTTGGCGTCGTAGAAGTGCGAGTAGTGAGTGTTGTCGCTGAAGCAGTCTTGCTCGTCTTcc                                                          >  1:101655/1‑149 (MQ=255)
                                                                                              gACCAGCGACGACAGGCTAGGGCCGGTAAGCTTGGTGCCGTCCGGGCGGGTGTACTCGCCCAGGTAGACGTTGCGGATGCCCTTGGCGTCGTAGAAGTGCGAGTAGTGAGTGTTGTCGCTGAAGCAGTCTTGCTCGTCTTCCGGCGAGt                                                   <  2:101655/149‑1 (MQ=255)
                                                                                                      acgacAGGCTAGGGCCGGTAAGCTTGGTGCCGTCCGGGCGGGTGTACTCGCCCAGGTAGACGTTGCGGATGCCCTTGGCGTCGTAGAAGTGCGa                                                                                                  <  4:61090/94‑1 (MQ=255)
                                                                                                      acgacAGGCTAGGGCCGGTAAGCTTGGTGCCGTCCGGGCGGGTGTACTCGCCCAGGTAGACGTTGCGGATGCCCTTGGCGTCGTAGAAGTGCGa                                                                                                  >  3:61090/1‑94 (MQ=255)
                                                                                                           aGGCTAGGGCCGGTAAGCTTGGTGCCGTCCGGGCGGGTGTACTCGCCCAGGTAGACGTTGCGGATGCCCTTGGCGTCGTAGAAGTGCGAGTAGTGAGTGTTGTCGCTGAAGCAGTCTTGCTCGTCTTCCGGCGAGTTGGCTTCCAGCg                                       >  5:209913/1‑148 (MQ=255)
                                                                                                           aGGCTAGGGCCGGTAAGCTTGGTGCCGTCCGGGCGGGTGTACTCGCCCAGGTAGACGTTGCGGATGCCCTTGGCGTCGTAGAAGTGCGAGTAGTGAGTGTTGTCGCTGAAGCAGTCTTGCTCGTCTTCCGGCGAGTTGGCTTCCAGCGa                                      <  7:215945/149‑1 (MQ=255)
                                                                                                                    ccGGTAAGCTTGGTGCCGTCCGGGCGGGTGTACTCGCCCAGGTAGACGTTGCGGATGCCCTTGGCGTCGTAGAAGTGCGAGTAGTGAGTGTTGTCGCTGAAGCAGTCTTGCTCGTCTTCCGGCGAGTTGGCTTCCAGCGAAACTTTCAt                             <  6:56363/149‑1 (MQ=255)
                                                                                                                    ccGGTAAGCTTGGTGCCGTCCGGGCGGGTGTACTCGCCCAGGTAGACGTTGCGGATGCCCTTGGCGTCGTAGAAGTGCGAGTAGTGAGTGTTGTCGCTGAAGCAGTCTTGCTCGTCTTCCGGCGAGTTGGCTTCCAGCGAAACTTTCAt                             >  1:8759/1‑149 (MQ=255)
                                                                                                                      ggTAAGCTTGGTGCCGTCCGGGCGGGTGTACTCGCCCAGGTAGACGTTGCGGATGCCCTTGGCGTCGTAGAAGTGCGAGTAGTGAGTGTTGTCGCTGAAGCAGTCTTGCTCGTCTTCCGGCGAGTTGGCTTCCAGCGAAACTTTCATgc                           <  2:8759/149‑1 (MQ=255)
                                                                                                                       gTAAGCTTGGTGCCGTCCGGGCGGGTGTACTCGCCCAGGTAGACGTTGCGGATGCCCTTGGCGTCGTAGAAGTGCGAGTAGTGAGTGTTGTCGCTGAAGCAGTCTTGCTCGTCTTCCGGCGAGTTGGCTTCCAGCGAAACTTTCATg                            <  7:91975/147‑1 (MQ=255)
                                                                                                                       gTAAGCTTGGTGCCGTCCGGGCGGGTGTACTCGCCCAGGTAGACGTTGCGGATGCCCTTGGCGTCGTAGAAGTGCGAGTAGTGAGTGTTGTCGCTGAAGCAGTCTTGCTCGTCTTCCGGCGAGTTGGCTTCCAGCGAAACTTTCATg                            >  8:91975/1‑147 (MQ=255)
                                                                                                                            cTTGGTGCCGTCCGGGCGGGTGTACTCGCCCAGGTAGACGTTGCGGATGCCCTTGGCGTCGTAGAAGTGCGAGTAGTGAGTGTTGTCGCTGAAGCAGTCTTGCTCGTCTTCCGGCGAGTTGGCTTCCAGCGAAACTTTCATGCGCTCgc                     >  3:261524/1‑149 (MQ=255)
                                                                                                                                gTGCCGTCCGGGCGGGTGTACTCGCCCAGGTAGACGTTGCGGATGCCCTTGGCGTCGTAGAAGTGCGAGTAGTGAGTGTTGTCGc                                                                                 >  7:227562/1‑85 (MQ=255)
                                                                                                                                gTGCCGTCCGGGCGGGTGTACTCGCCCAGGTAGACGTTGCGGATGCCCTTGGCGTCGTAGAAGTGCGAGTAGTGAGTGTTGTCGc                                                                                 <  8:227562/85‑1 (MQ=255)
                                                                                                                                gTGCCGTCCGGGCGGGTGTACTCGCCCAGGTAGACGTTGCGGATGCCCTTGGCGTCGTAGAAGTGCGAGTAGTGAGTGTTGTCGCTGAAGCAGTCTTGCTCGTCTTCCGGCGAGTTGGCTTCCAGCGAAACTTTCATGCGCTCgccagc                 <  4:30620/149‑1 (MQ=255)
                                                                                                                                gTGCCGTCCGGGCGGGTGTACTCGCCCAGGTAGACGTTGCGGATGCCCTTGGCGTCGTAGAAGTGCGAGTAGTGAGTGTTGTCGCTGAAGCAGTCTTGCTCGTCTTCCGGCGAGTTGGCTTCCAGCGAAACTTTCATGCGCTCgccagc                 <  8:31999/149‑1 (MQ=255)
                                                                                                                                     acctgggcgagTACACCCGCCCAGGTAGACGTTGCGGATGCCCTTGGCGTCGTAGAAGTGCGAGTAGTGAGTGTTGTCGCTGAAGCAGTCTTGCTCGTCTTCCGGCGAGTTGGCTTCCAGCGAAACTTTCATGCGCTCGCCAGCCAGtt            >  3:21609/5‑149 (MQ=255)
                                                                                                                                       ctgggcgagTACACCCGCCCAGGTAGACGTTGCGGATGCCCTTGGCGTCGTAGAAGTGCGAGTAGTGAGTGTTGTCGCTGAAGCAGTCTTGCTCGTCTTCCGGCGAGTTg                                                 >  4:139524/3‑110 (MQ=255)
                                                                                                                                       ctgggcgagTACACCCGCCCAGGTAGACGTTGCGGATGCCCTTGGCGTCGTAGAAGTGCGAGTAGTGAGTGTTGTCGCTGAAGCAGTCTTGCTCGTCTTCCGGCGAGTTg                                                 <  3:139524/108‑1 (MQ=255)
                                                                                                                                                tgtACTCGCCCAGGTAGACGTTGCGGATGCCCTTGGCGTCGTAGAAGTGCGAGTAGTGAGTGTTGTCGCTGAAGCAGTCTTGCTCGTCTTCCGGCGAGTTGGCTTCCAGCGAAACTTTCATGCGCTCGCCAGCCAGTTCGCCCAGCGa  >  5:220154/1‑148 (MQ=255)
                                                                                                                                                tgtACTCGCCCAGGTAGACGTTGCGGATGCCCTTGGCGTCGTAGAAGTGCGAGTAGTGAGTGTTGTCGCTGAAGCAGTCTTGCTCGTCTTCCGGCGAGTTGGCTTCCAGCGAAACTTTCATGCGCTCGCCAGCCAGTTCGCCCAGCGa  >  4:210833/1‑148 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
CTTTGAGGGCGTGGTCTACGATCACCTGAAGCTTGGCCTCGGTGGCTTCCAGATCGGCCTTGAGGGCTGCATCGGCGGCCGGGTCAACCTTGGCGACCAGCGACGACAGGCTAGGGCCGGTAAGCTTGGTGCCGTCCGGGCGGGTGTACTCGCCCAGGTAGACGTTGCGGATGCCCTTGGCGTCGTAGAAGTGCGAGTAGTGAGTGTTGTCGCTGAAGCAGTCTTGCTCGTCTTCCGGCGAGTTGGCTTCCAGCGAAACTTTCATGCGCTCGCCAGCCAGTTCGCCCAGCGA  >  NC_002947/1054664‑1054955

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑