Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,054,813 T→C 8.7% E340G (GAG→GGG)  PP_0913 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,054,8130TC 8.7% 128.4 / 3.0 46E340G (GAG→GGG) PP_0913hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (19/23);  new base C (2/2);  total (21/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGGCGTGGTCTACGATCACCTGAAGCTTGGCCTCGGTGGCTTCCAGATCGGCCTTGAGGGCTGCATCGGCGGCCGGGTCAACCTTGGCGACCAGCGACGACAGGCTAGGGCCGGTAAGCTTGGTGCCGTCCGGGCGGGTGTACTCGCCCAGGTAGACGTTGCGGATGCCCTTGGCGTCGTAGAAGTGCGAGTAGTGAGTGTTGTCGCTGAAGCAGTCTTGCTCGTCTTCCGGCGAGTTGGCTTCCAGCGAAACTTTCATGCGCTCGCCAGCCAGTTCGCCCAGCGACAGGCT  >  NC_002947/1054670‑1054961
                                                                                                                                               |                                                                                                                                                    
gggCGTGGTCTACGATCACCTGAAGCTTGGCCTCGGTGGCTTCCAGATCGGCCTTGAGGGCTGCATCGGCGGCCGGGTCAACCTTGGCGACCAGCGACGACAGGCTAGGGCCGGTAAGCTTGGTGCCGTCCGGGCGGGTGTACTCGccc                                                                                                                                                 <  2:202621/149‑1 (MQ=255)
 ggCGTGGTCTACGATCACCTGAAGCTTGGCCTCGGTGGCTTCCAGATCGGCCTTGAGGGCTGCATCGGCGGCCGGGTCAACCTTGGCGACCAGCGACGACAGGCTAGGGCCGGTAAGCTTGGTGCCGTCCGGGCGGGTGTACTCGcc                                                                                                                                                  >  2:260028/1‑147 (MQ=255)
   cGTGGTCTACGATCACCTGAAGCTTGGCCTCGGTGGCTTCCAGATCGGCCTTGAGGGCTGCATCGGCGGCCGGGTCAACCTTGGCGACCAGCGACGACAGGCTAGGGCCGGTAAGCTTGGTGCCGTCCGGGCGGGTGTACTCGCCCAgg                                                                                                                                              <  5:245409/149‑1 (MQ=255)
    gTGGTCTACGATCACCTGAAGCTTGGCCTCGGTCGCTTCCAGCTCGGCCTTGAGGGCTGCATCGGCGGCCGGGTCAACCTTGGCGACCAGCGACGACAGGCTAGGGCCGGTAAGCTTGGTGCCGTCCGGGCGAGTACACCCGcccggac                                                                                                                                             <  6:92137/149‑5 (MQ=255)
         cTACGATCACCTGAAGCTTGGCCTCGGTGGCTTCCAGATCGGCCTTGAGGGCTGCATCGGCGGCCGGGTCAACCTTGGCGACCAGCGACGACAGGCTAGGGCCGGTAAGCTTGGTGCCGTCCGGGCGGGTGTACTCGCCCAGGTAGACg                                                                                                                                        >  6:176394/1‑149 (MQ=255)
             gATCACCTGAAGCTTGGCCTCGGTGGCTTCCAGATCGGCCTTGAGGGCTGCATCGGCGGCCGGGTCAACCTTGGCGACCAGCGACGACAGGCTAGGGCCGGTAAGCTTGGTGCCGTCCGGGCGGGTGTACTCGCCCAGGTAGACGTTGc                                                                                                                                    >  4:62739/1‑149 (MQ=255)
              aTCACCTGAAGCTTGGCCTCGGTGGCTTCCAGATCGGCCTTGAGGGCTGCATCGGCGGCCGGGTCAACCTTGGCGACCAGCGACGACAGGCTAGGGCCGGTAAGCTTGGTGCCGTCCGGGGGGGTGTACTCGCCCAGGTAGACGTTGCg                                                                                                                                   <  8:157157/149‑1 (MQ=255)
               tCACCTGAAGCTTGGCCTCGGTGGCTTCCAGATCGGCCTTGAGGGCTGCATCGGCGGCCGGGTCAACCTTGGCGACCAGCGACGACAGGCTAGGGCCGGTAAGCTTGGTGCCGTCCGGGCGGGTGTACTCGCCCAGGTAGACGTTGCg                                                                                                                                   >  7:177649/1‑148 (MQ=255)
                        gCTTGGCCTCGGTGGCTTCCAGATCGGCCTTGAGGGCTGCATCGGCGGCCGGGTCAACCTTGGCGACCAGCGACGACAGGCTAGGGCCGGTAAGCTTGGTGCCGTCCGGGCGGGTGTACTCGCCCAGGTAGACGTTGCGGATGCCCTTg                                                                                                                         <  5:176394/149‑1 (MQ=255)
                                       cTTCCAGATCGGCCTTGAGGGCTGCATCGGCGGCCGGGTCAACCTTGGCGACCAGCGACGACAGGCTAGGGCCGGTAAGCTTGGTGCCGTCCGGGCGGGTGTACTCGCCCAGGTAGACGTTGCGGATGCCCTTGGCGTCGTAGAAGTGc                                                                                                          >  4:87661/1‑149 (MQ=255)
                                             gATCGGCCTTGAGGGCTGCATCGGCGGCCGGGTCAACCTTGGCGACCAGCGACGACAGGCTAGGGCCGGTAAGCTTGGTGCCGTCCGGGCGGGTGTACTCGCCCAGGTAGACGTTGCGGATGCCCTTGGCGTCGTAGAAGTGCGagtag                                                                                                    <  3:87661/149‑1 (MQ=255)
                                              aTCGGCCTTGAGGGCTGCATCGGCGGCCGGGTCAACCTTGGCGACCAGCGACGACAGGCTAGGGCCGGTAAGCTTGGTGCCGTCCGGGCGGGTGTACTCGCCCAGGTAGACGTTGCGGATGCCCTTGGCGTCGTAGAAGTGCGagtagt                                                                                                   <  7:24088/149‑1 (MQ=255)
                                               tCGGCCTTGAGGGCTGCATCGGCGGCCGGGTCAACCTTGGCGACCAGCGACGACAGGCTAGGGCCGGTAAGCTTGGTGCCGTCCGGGCGGGTGTACTCGCCCAGGTAGACGTTGCGGATGCCCTTGGCGTCGTAGAAGTGCGagtagt                                                                                                   <  7:183301/148‑1 (MQ=255)
                                                 ggCCTTGAGGGCTGCATCGGCGGCCGGGTCAACCTTGGCGACCAGCGACGACAGGCTAGGGCCGGTAAGCTTGGTGCCGTCCGGGCGGGTGTACTCGCCCAGGTAGACGTTGCGGATGCCCTTGGCGTCGTAGAAGTGCGAGTagtga                                                                                                 <  3:62739/148‑1 (MQ=255)
                                                          ggCTGCATCGGCGGCCGGGTCAACCTTGGCGACCAGCGACGACAGGCTAGGGCCGGTAAGCTTGGTGCCGTCCGGGCGGGTGTACTCGCCCAGGTAGACGTTGCGGATGCCCTTGGCGTCGTAGAAGTGCGAGTagtga                                                                                                 <  7:49095/139‑1 (MQ=255)
                                                          ggCTGCATCGGCGGCCGGGTCAACCTTGGCGACCAGCGACGACAGGCTAGGGCCGGTAAGCTTGGTGCCGTCCGGGCGGGTGTACTCGCCCAGGTAGACGTTGCGGATGCCCTTGGCGTCGTAGAAGTGCGAGTagtga                                                                                                 >  8:49095/1‑139 (MQ=255)
                                                              gCATCGGCGGCCGGGTCAACCTTGGCGACCAGCGACGACAGGCTAGGGCCGGTAAGCTTGGTGCCGTCCGGGCGGGTGTACTCGCCCAGGTAGACGTTGCGGATGCCCTTGGCGTCGTAGAAGTGCGAGTAGTGAGTGTTGTCGCTGaa                                                                                   <  3:216980/149‑1 (MQ=255)
                                                               gaTCGGCTGCCGTGGAAACCTTCGCCACCAGCGACGACACGCTAGGGCCGGTAAGCTTGGTGCCGTCCGGGCGTGTGTACTCGCCCAGGTAGACGTTGCGTATGCCGTTGGCGTCGTAGAAGTTCGAGTAGTCAGTGTTTGCGCTGAAg                                                                                  <  2:150772/148‑1 (MQ=255)
                                                                      ggcCGGGTCAACCTTGGCGACCAGCGACGACAGGCTAGGGCCGGTAAGCTTGGTGCCGTCCGGGCGGGTGTACTCGCCCAGGTAGACGTTGCGGATGCCCTTGGCGTCGTAGAAGTGCGAGTAGTGAGTGTTGTCGCTGAAGCAGTCtt                                                                           <  8:177649/149‑1 (MQ=255)
                                                                         cGGGTCAACCTTGGCGACCAGCGACGACAGGCTAGGGCCGGTAAGCTTGGTGCCGTCCGGGCGGGTGTACTCGCCCAGGTAGACGTTGCGGATGCCCTTGGCGTCGTAGAAGTGCGAGTAGTGAGTGTTGTCGCTGAAGCAGTCTTGc                                                                         >  6:66995/1‑148 (MQ=255)
                                                                          gggTCAACCTTGGCGACCAGCGACGACAGGCTAGGGCCGGTAAGCTTGGTGCCGTCCGGGCGGGTGTACTCGCCCAGGTAGACGTTGCGGATGCCCTTGGCGTCGTAGAAGTGCGAGTAGTGAGTGTTGTCGCTGAAGCAGTCTTGCTc                                                                       <  1:260028/149‑1 (MQ=255)
                                                                            gTCAACCTTGGCGACCAGCGACGACAGGCTAGGGCCGGTAAGCTTGGTGCCGTCCGGGCGGGTGTACTCGCCCAGGTAGACGTTGCGGATGCCCTTGGCGTCGTAGAAGTGCGAGTAGTGAGTGTTGTCGCTGAAGCAGTCTTGCTCGt                                                                     <  5:66995/149‑1 (MQ=255)
                                                                             tCAACCTTGGCGACCAGCGACGACAGGCTAGGGCCGGTAAGCTTGGTGCCGTCCGGGCGGGTGTACTCGCCCAGGTAGACGTTGCGGATGCCCTTGGCGTCGTAGAAGTGCGAGTAGTGAGTGTTGTCGCTGAAGCAGTCTTGCTCGTc                                                                    >  5:56363/1‑149 (MQ=255)
                                                                               aaCCTTGGCGACCAGCGACGACAGGCTAGGGCCGGTAAGCTTGGTGCCGTCCGGGCGGGTGTACTCGCCCAGGTAGACGTTGCGGATGCCCTTGGCGTCGTAGAAGTGCGAGTAGTGAGTGTTGTCGCTGAAGCAGTCTTGCTCGTCtt                                                                  >  7:31999/1‑149 (MQ=255)
                                                                               aaCCTTGGCGACCAGCGACGACAGGCTAGGGCCGGTAAGCTTGGTGCCGTCCGGGCGGGTGTACTCGCCCAGGTAGACGTTGCGGATGCCCTTGGCGTCGTAGAAGTGCGAGTAGTGAGTGTTGTCGCTGAAGCAGTCTTGCTCGTCtt                                                                  >  3:30620/1‑149 (MQ=255)
                                                                                 ccTTGGCGACCAGCGACGACAGGCTAGGGCCGGTAAGCTTGGTGCCGTCCGGGCGGGTGTACTCGCCCAGGTAGACGTTGCGGATGCCCTTGGCGTCGTAGAAGTGCGAGTAGTGAGTGTTGTCGCTGAAGCAGTCTTGCTCGTCTTcc                                                                >  1:101655/1‑149 (MQ=255)
                                                                                        gACCAGCGACGACAGGCTAGGGCCGGTAAGCTTGGTGCCGTCCGGGCGGGTGTACTCGCCCAGGTAGACGTTGCGGATGCCCTTGGCGTCGTAGAAGTGCGAGTAGTGAGTGTTGTCGCTGAAGCAGTCTTGCTCGTCTTCCGGCGAGt                                                         <  2:101655/149‑1 (MQ=255)
                                                                                                acgacAGGCTAGGGCCGGTAAGCTTGGTGCCGTCCGGGCGGGTGTACTCGCCCAGGTAGACGTTGCGGATGCCCTTGGCGTCGTAGAAGTGCGa                                                                                                        <  4:61090/94‑1 (MQ=255)
                                                                                                acgacAGGCTAGGGCCGGTAAGCTTGGTGCCGTCCGGGCGGGTGTACTCGCCCAGGTAGACGTTGCGGATGCCCTTGGCGTCGTAGAAGTGCGa                                                                                                        >  3:61090/1‑94 (MQ=255)
                                                                                                     aGGCTAGGGCCGGTAAGCTTGGTGCCGTCCGGGCGGGTGTACTCGCCCAGGTAGACGTTGCGGATGCCCTTGGCGTCGTAGAAGTGCGAGTAGTGAGTGTTGTCGCTGAAGCAGTCTTGCTCGTCTTCCGGCGAGTTGGCTTCCAGCg                                             >  5:209913/1‑148 (MQ=255)
                                                                                                     aGGCTAGGGCCGGTAAGCTTGGTGCCGTCCGGGCGGGTGTACTCGCCCAGGTAGACGTTGCGGATGCCCTTGGCGTCGTAGAAGTGCGAGTAGTGAGTGTTGTCGCTGAAGCAGTCTTGCTCGTCTTCCGGCGAGTTGGCTTCCAGCGa                                            <  7:215945/149‑1 (MQ=255)
                                                                                                              ccGGTAAGCTTGGTGCCGTCCGGGCGGGTGTACTCGCCCAGGTAGACGTTGCGGATGCCCTTGGCGTCGTAGAAGTGCGAGTAGTGAGTGTTGTCGCTGAAGCAGTCTTGCTCGTCTTCCGGCGAGTTGGCTTCCAGCGAAACTTTCAt                                   <  6:56363/149‑1 (MQ=255)
                                                                                                              ccGGTAAGCTTGGTGCCGTCCGGGCGGGTGTACTCGCCCAGGTAGACGTTGCGGATGCCCTTGGCGTCGTAGAAGTGCGAGTAGTGAGTGTTGTCGCTGAAGCAGTCTTGCTCGTCTTCCGGCGAGTTGGCTTCCAGCGAAACTTTCAt                                   >  1:8759/1‑149 (MQ=255)
                                                                                                                ggTAAGCTTGGTGCCGTCCGGGCGGGTGTACTCGCCCAGGTAGACGTTGCGGATGCCCTTGGCGTCGTAGAAGTGCGAGTAGTGAGTGTTGTCGCTGAAGCAGTCTTGCTCGTCTTCCGGCGAGTTGGCTTCCAGCGAAACTTTCATgc                                 <  2:8759/149‑1 (MQ=255)
                                                                                                                 gTAAGCTTGGTGCCGTCCGGGCGGGTGTACTCGCCCAGGTAGACGTTGCGGATGCCCTTGGCGTCGTAGAAGTGCGAGTAGTGAGTGTTGTCGCTGAAGCAGTCTTGCTCGTCTTCCGGCGAGTTGGCTTCCAGCGAAACTTTCATg                                  >  8:91975/1‑147 (MQ=255)
                                                                                                                 gTAAGCTTGGTGCCGTCCGGGCGGGTGTACTCGCCCAGGTAGACGTTGCGGATGCCCTTGGCGTCGTAGAAGTGCGAGTAGTGAGTGTTGTCGCTGAAGCAGTCTTGCTCGTCTTCCGGCGAGTTGGCTTCCAGCGAAACTTTCATg                                  <  7:91975/147‑1 (MQ=255)
                                                                                                                      cTTGGTGCCGTCCGGGCGGGTGTACTCGCCCAGGTAGACGTTGCGGATGCCCTTGGCGTCGTAGAAGTGCGAGTAGTGAGTGTTGTCGCTGAAGCAGTCTTGCTCGTCTTCCGGCGAGTTGGCTTCCAGCGAAACTTTCATGCGCTCgc                           >  3:261524/1‑149 (MQ=255)
                                                                                                                          gTGCCGTCCGGGCGGGTGTACTCGCCCAGGTAGACGTTGCGGATGCCCTTGGCGTCGTAGAAGTGCGAGTAGTGAGTGTTGTCGc                                                                                       <  8:227562/85‑1 (MQ=255)
                                                                                                                          gTGCCGTCCGGGCGGGTGTACTCGCCCAGGTAGACGTTGCGGATGCCCTTGGCGTCGTAGAAGTGCGAGTAGTGAGTGTTGTCGc                                                                                       >  7:227562/1‑85 (MQ=255)
                                                                                                                          gTGCCGTCCGGGCGGGTGTACTCGCCCAGGTAGACGTTGCGGATGCCCTTGGCGTCGTAGAAGTGCGAGTAGTGAGTGTTGTCGCTGAAGCAGTCTTGCTCGTCTTCCGGCGAGTTGGCTTCCAGCGAAACTTTCATGCGCTCgccagc                       <  8:31999/149‑1 (MQ=255)
                                                                                                                          gTGCCGTCCGGGCGGGTGTACTCGCCCAGGTAGACGTTGCGGATGCCCTTGGCGTCGTAGAAGTGCGAGTAGTGAGTGTTGTCGCTGAAGCAGTCTTGCTCGTCTTCCGGCGAGTTGGCTTCCAGCGAAACTTTCATGCGCTCgccagc                       <  4:30620/149‑1 (MQ=255)
                                                                                                                               acctgggcgagTACACCCGCCCAGGTAGACGTTGCGGATGCCCTTGGCGTCGTAGAAGTGCGAGTAGTGAGTGTTGTCGCTGAAGCAGTCTTGCTCGTCTTCCGGCGAGTTGGCTTCCAGCGAAACTTTCATGCGCTCGCCAGCCAGtt                  >  3:21609/5‑149 (MQ=255)
                                                                                                                                 ctgggcgagTACACCCGCCCAGGTAGACGTTGCGGATGCCCTTGGCGTCGTAGAAGTGCGAGTAGTGAGTGTTGTCGCTGAAGCAGTCTTGCTCGTCTTCCGGCGAGTTg                                                       <  3:139524/108‑1 (MQ=255)
                                                                                                                                 ctgggcgagTACACCCGCCCAGGTAGACGTTGCGGATGCCCTTGGCGTCGTAGAAGTGCGAGTAGTGAGTGTTGTCGCTGAAGCAGTCTTGCTCGTCTTCCGGCGAGTTg                                                       >  4:139524/3‑110 (MQ=255)
                                                                                                                                          tgtACTCGCCCAGGTAGACGTTGCGGATGCCCTTGGCGTCGTAGAAGTGCGAGTAGTGAGTGTTGTCGCTGAAGCAGTCTTGCTCGTCTTCCGGCGAGTTGGCTTCCAGCGAAACTTTCATGCGCTCGCCAGCCAGTTCGCCCAGCGa        >  5:220154/1‑148 (MQ=255)
                                                                                                                                          tgtACTCGCCCAGGTAGACGTTGCGGATGCCCTTGGCGTCGTAGAAGTGCGAGTAGTGAGTGTTGTCGCTGAAGCAGTCTTGCTCGTCTTCCGGCGAGTTGGCTTCCAGCGAAACTTTCATGCGCTCGCCAGCCAGTTCGCCCAGCGa        >  4:210833/1‑148 (MQ=255)
                                                                                                                                               tCGCCCAGGTAGACGTTGCGGATGCCCTTGGCGTCGTAGAAGTGCGAGTAGTGAGTGTTGTCGCTGAAGCAGTCTTGCTCGTCTTCCGGCGAGTTGGCTTCCAGCGAAACTTTCATGCGCTCGCCAGCCAGTTCGCCCAGCGACAGGCt  >  2:240168/1‑149 (MQ=255)
                                                                                                                                               |                                                                                                                                                    
GGGCGTGGTCTACGATCACCTGAAGCTTGGCCTCGGTGGCTTCCAGATCGGCCTTGAGGGCTGCATCGGCGGCCGGGTCAACCTTGGCGACCAGCGACGACAGGCTAGGGCCGGTAAGCTTGGTGCCGTCCGGGCGGGTGTACTCGCCCAGGTAGACGTTGCGGATGCCCTTGGCGTCGTAGAAGTGCGAGTAGTGAGTGTTGTCGCTGAAGCAGTCTTGCTCGTCTTCCGGCGAGTTGGCTTCCAGCGAAACTTTCATGCGCTCGCCAGCCAGTTCGCCCAGCGACAGGCT  >  NC_002947/1054670‑1054961

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑