Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,070,260	G→C	17.9%	intergenic (+60/+82)	aroP‑I → / ← PP_0928	aromatic amino acid transport protein/K+‑dependent Na+/Ca+ exchanger‑like protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,070,260	0	G	C	17.9%	126.5 / 17.8	50	intergenic (+60/+82)	aroP‑I/PP_0928	aromatic amino acid transport protein/K+‑dependent Na+/Ca+ exchanger‑like protein
Reads supporting (aligned to +/- strand):  ref base G (18/23);  new base C (4/5);  total (22/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CATCTCGGTAATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑TGT‑‑GA‑‑TCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAGCACGCACAGTGGTGTACAGCAACACCGTAGCGAGCACCGGCAGTAC  >  NC_002947/1070116‑1070407                                                                                                                                                       |                                                                                                                                                    cATCTCGGTAATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑TGTGAGA‑‑TCGAGCGCcgcgcg                                                                                                                                                   <  1:232863/149‑1 (MQ=255)        gTAATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑TGTGAGA‑‑TCGAGCGCCGCGCGGgcggcg                                                                                                                                            >  7:251483/1‑149 (MQ=255)        gTAATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑TGTGAGA‑‑TCGAGCGCCGCCCGCgcggcg                                                                                                                                            >  7:148749/1‑149 (MQ=39)            tGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑TGTGAGA‑‑TCGAGCGCCGCGCGGGCGGCGCTCg                                                                                                                                        <  3:205203/149‑1 (MQ=255)            tGCTGATCCCCGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCTTTAGGCTTTCATCGCC‑‑TGTGAGA‑‑TCGAGCGCCGCCCGCGCGGCGCTCg                                                                                                                                        <  4:99808/149‑1 (MQ=25)              cTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑TGTGAGA‑‑TCGAGCGCCGCCCGCGCGGCGCTCGAt                                                                                                                                      <  8:148749/149‑1 (MQ=38)                  tCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑TGTGAGA‑‑TCGAGCGCCGCGCGGGCGGCGCTCGATCTca                                                                                                                                  <  7:123010/149‑1 (MQ=255)                    ccGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑TGTGAGA‑‑TCGAGCGCCGCGCGGGCGGCGCTCGATCTcac                                                                                                                                 <  5:115137/148‑1 (MQ=255)                     cGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑TGTGAGA‑‑TCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAg                                                                                                                               >  8:84656/1‑149 (MQ=255)                                gctgtgctgtGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑TGTGAGA‑‑TCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAg                                                                                                                     <  6:122221/148‑1 (MQ=255)                                            gtggCTTGCAAGCTGACGAAGGCTCGCCAGGCCAAGGGCGGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑TGTGAGA‑‑TCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGc                                                                                                        <  2:120993/149‑1 (MQ=38)                                               gCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑TGTGAGA‑‑TCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGt                                                                                                      <  7:84656/148‑1 (MQ=255)                                                 tttCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑TGTGAGA‑‑TCGAGCGCCGCCCGCGCGGCGCTCGa                                                                                                                                       >  2:18448/1‑113 (MQ=37)                                                 tttCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑TGTGAGA‑‑TCGAGCGCCGCCCGCGCGGCGCTCGa                                                                                                                                       <  1:18448/113‑1 (MQ=37)                                                        cTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑TGTGAGA‑‑TCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGccc                                                                                            >  5:202334/1‑149 (MQ=255)                                                         tGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑TGTGAGA‑‑TCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCt                                                                                           >  6:228928/1‑149 (MQ=255)                                                          gaagaaGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑TGTGAGA‑‑TCGAGCGCCGCGCGGGCGGCGCTCGATCTca                                                                                                                                  <  6:30364/109‑1 (MQ=255)                                                          gaagaaGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑TGTGAGA‑‑TCGAGCGCCGCGCGGGCGGCGCTCGATCTca                                                                                                                                  >  5:30364/1‑109 (MQ=255)                                                          gaagaaGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑TGTGAGA‑‑TCGAGCGCCGCGCGGGCGGCGCTCGATCTca                                                                                                                                  >  5:34362/1‑109 (MQ=255)                                                          gaagaaGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑TGTGAGA‑‑TCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAg                                                                                                                     <  2:150705/122‑1 (MQ=255)                                                          gaagaaGGCTCGCCAGGCCAAGGGCTGAGCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑TGTGAGA‑‑TCGAGCGCCGCGCGGGCGGCGCTCGATCTca                                                                                                                                  <  6:34362/109‑1 (MQ=37)                                                          gaagaaGGCTCGCCAGGCCAAGGGCTGAACGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑TGTGAGA‑‑TCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAg                                                                                                                     >  1:150705/1‑122 (MQ=37)                                                              aagGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑TGTGAGA‑‑TCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGAcgc                                                                                      <  8:251483/149‑1 (MQ=255)                                                                   tCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑TGTGAGA‑‑TCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTcc                                                                                 >  8:136052/1‑149 (MQ=255)                                                                      ccAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑TGTGAGA‑‑TCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCt                                                                              >  1:114390/1‑149 (MQ=255)                                                                             aaGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑TGTGAGA‑‑TCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGc                                                                       <  1:225838/149‑1 (MQ=255)                                                                              aGGGCGGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGGTTTCGGCGCCGGTGA‑‑GA‑‑TCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGcc                                                                      <  2:223844/149‑1 (MQ=38)                                                                                ggCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑TGTGAGA‑‑TCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGcccc                                                                    >  2:182002/1‑149 (MQ=255)                                                                                 gCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑TGTGAGA‑‑TCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCt                                                                   >  5:276544/1‑149 (MQ=255)                                                                                 gCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑TGTGAGA‑‑TCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTACCCTTCCATGCCCCt                                                                   >  7:70431/1‑149 (MQ=255)                                                                                     aTCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑TGTGAGA‑‑TCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCa                                                                 <  1:182002/147‑1 (MQ=255)                                                                                        gggTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑TGTGAGA‑‑TCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAAtgct                                                            <  6:276544/149‑1 (MQ=255)                                                                                        gggTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑TGTGAGA‑‑TCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAAtgct                                                            >  1:165450/1‑149 (MQ=255)                                                                                        gggTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑TGTGAGA‑‑TCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAAtgct                                                            <  5:71546/149‑1 (MQ=255)                                                                                                 cGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑TGTGAGA‑‑TCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTggcggc                                                                                              >  3:29165/1‑106 (MQ=255)                                                                                                 cGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑TGTGAGA‑‑TCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTggcggc                                                                                              <  4:29165/106‑1 (MQ=255)                                                                                                     aGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑TGTGAGA‑‑TCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAg                                                >  3:235375/1‑148 (MQ=255)                                                                                                      ggggTATGCCGTTAGGTTTTCGGCGCC‑‑TGTGAGA‑‑TCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAgca                                              <  2:114390/149‑1 (MQ=255)                                                                                                       gggTATGCCGTTAGGTTTTCGGCGCC‑‑TGTGAGA‑‑TCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAgcac                                             >  6:130887/1‑149 (MQ=255)                                                                                                         gcaTGCCTTTAGGCTTTCATCGCC‑‑TGTGAGA‑‑TCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGCCGAAAACCTAACGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAgcacg                                            >  4:246069/3‑148 (MQ=17)                                                                                                            tGCCGTTAGGTTTTCGGCGCC‑‑TGTGAGA‑‑TCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAgcacgcac                                         <  4:235375/148‑1 (MQ=255)                                                                                                               ctttAGGCTTTCATCGCC‑‑TGTGAGA‑‑TCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGCCGAAAACCTAACGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAGCACGCACAgtg                                     <  3:246069/147‑1 (MQ=18)                                                                                                               cGTTAGGTTTTCGGCGCC‑‑TGTGAGA‑‑TCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAGCACGCACAgtg                                     <  5:228928/149‑1 (MQ=255)                                                                                                                gTTAGGTTTTCGGCGCC‑‑TGTGAGA‑‑TCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAGCACGcaca                                        >  1:30423/1‑145 (MQ=255)                                                                                                                gTTAGGTTTTCGGCGCC‑‑TGTGAGA‑‑TCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAGCACGcaca                                        <  2:30423/145‑1 (MQ=255)                                                                                                                   aGGTTTTCGGCGCC‑‑TGTGAGA‑‑TCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAGCACGCACAgttgtg                                  <  6:202334/148‑1 (MQ=255)                                                                                                                           ggCGCC‑‑TGT‑‑GCTCTCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAGCACGCACAGTGGTGTACAGCaa                          <  4:10467/148‑1 (MQ=255)                                                                                                                            gcgcC‑‑TGTGAGA‑‑TCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAGCACGCACAGTGGTGTACAGCAaca                        >  1:219831/1‑149 (MQ=255)                                                                                                                                     tgagA‑‑TCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAGCACGCACAGTGGTGTACAGCAACACCGTAg                  <  7:136052/145‑1 (MQ=255)                                                                                                                                               aGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGCCGAAAACCTAACGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAAtgct                                                            <  6:129558/98‑1 (MQ=25)                                                                                                                                               aGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGCCGAAAACCTAACGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAAtgct                                                            >  5:129558/1‑98 (MQ=25)                                                                                                                                                    cgcgcgGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAGCACGCACAGTGGTGTACAGCAACACCGTAGCGAGCACCGGCAg     >  1:108886/1‑148 (MQ=255)                                                                                                                                                      cgcgGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAGCACGCACAGTGGTGTACAGCAACACCGTAGCGAGCACCGGCAGTAc  <  1:186460/149‑1 (MQ=255)                                                                                                                                                       |                                                                                                                                                    CATCTCGGTAATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑TGT‑‑GA‑‑TCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAGCACGCACAGTGGTGTACAGCAACACCGTAGCGAGCACCGGCAGTAC  >  NC_002947/1070116‑1070407

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑