Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,071,038 G→C 10.7% H116Q (CAC→CAG)  PP_0928 ← K+‑dependent Na+/Ca+ exchanger‑like protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,071,0380GC10.7% 133.0 / 4.3 46H116Q (CAC→CAG) PP_0928K+‑dependent Na+/Ca+ exchanger‑like protein
Reads supporting (aligned to +/- strand):  ref base G (18/23);  new base C (2/3);  total (20/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGCGTACCTGACCAGAAGCGCCCGGCCCCGGACCTGGCGGCGTCCGGGGCAGGGTAGGTGCGTGCGTAATGGCGCGACTGATGCCAGAGCATTGCCAGGTAACCGGCCAGCCCCAGCAATAGCAGTGCCCCTTCAACCCTGCCCAGGTGGGCATTGGCGGACAGCGCATACACCAGCCCGCTGGCAAGGATCATCAGGGGAATGTCCAGCCGCACCAGCTGGCGTGACACCCGCAGCGGGATGATCAGCGCGGCCAGGCCGAGAATGACCAGTACGTTGAAGATGTTGCTG  >  NC_002947/1070892‑1071182
                                                                                                                                                  |                                                                                                                                                
agcGTACCTGACCAGAAGCGCCCGGCCCCGGACCTGGCGGCGTCCGGCGCCGGGGAGGGGCGTGCGTAATGGCGCGCCTGCTGCCAGAGCAGTGCCAGGTCACCGGCCAGCCCCAGCCATAGCAGGGCCCCTTCACCCCTGCCCAgggc                                                                                                                                                >  5:54712/1‑147 (MQ=255)
    tACCTGACCAGAAGCGCCCGGCCCCGGACCTGGCGGCGTCCGGGGCAGGGTAGGTGCGTGCGTAATGGCGCGACTGATGCCAGAGCATTGCCAGGTAACCGGCCAGCCCCAGCAATAGCAGTGCCCCTTCAACCCTGCCCACCTGGGCa                                                                                                                                            <  7:20659/149‑1 (MQ=255)
     aCCTGACCAGAAGCGCCCGGCCCCGGACCTGGCGGCGTCCGGGGCAGGGTAGGTGCGTGCGTAATGGCGCGACTGATGCCAGAGCATTGCCAGGTAACCGGCCAGCCCCAGCAATAGCAGTGCCCCTTCAACCCTGCCCAGGTGGGCAt                                                                                                                                           >  1:187361/1‑149 (MQ=255)
       cTGACCAGAAGCGCCCGGCCCCGGACCTGGCGGCGTCCGGGGCAGGGTAGGTGCGTGCGTAATGGCGCGACTGATGCCAGAGCATTGCCAGGTAACCGGCCAGCCCCAGCAATAGCAGTGCCCCTTCAACCCTGCCCAGGTGGGCATTg                                                                                                                                         >  6:193071/1‑149 (MQ=255)
       cTGACCAGAAGCGCCCGGCCCCGGACCTGGCGGCGTCCGGGGCAGGGTAGGTGCGTGCGTAATGGCGCGACTGATGCCAGAGCATTGCCAGGTAACCGGCCAGCCCCAGCAATAGCAGTGCCCCTTCAACCCTGCCCACCTGGGCaggg                                                                                                                                         <  2:220068/149‑4 (MQ=255)
            cAGAAGCGCCCGGCCCCGGACCTGGCGGCGTCGGGGGCAGGGTAGGTGCGTGCGTAATGGCGCGACTGATGCCTGAGCATTGCCAGGTAACCGGCCAGCCCCAGCAATAGCAGTGCCCCTTCACCCCTGCCCAGGTGGGCATTGGCGGa                                                                                                                                    <  6:54712/149‑1 (MQ=255)
               aaGCGCCCGGCCCCGGACCTGGCGGCGTCCGGGGCAGGGTAGGTGCGTGCGTAATGGCGCGACTGATGCCAGAGCATTGCCAGGTAACCGGCCAGCCCCAGCAATAGCAGTGCCCCTTCAACCCTGCCCAGGTGGGCATTGGCGGACAg                                                                                                                                 <  5:193071/149‑1 (MQ=255)
               aaGCGCCCGGCCCCGGACCTGGCGGCGTCCGGGGCAGGGTAGGTGCGTGCGTAATGGCGCGACTGATGCCAGAGCATTGCCAGGTAACCGGCCAGCCCCAGCAATAGCAGTGCCCCTTCAACCCTGCCCAGGTGGGCATTGGCGGACAg                                                                                                                                 <  8:184900/149‑1 (MQ=255)
                  cgcCCGGCCCCGGACCTGGCGGCGTCCGGGGCAGGGTAGGTGCGTGCGTAATGGCGCGACTGATGCCAGAGCATTGCCAGGTAACCGGCCAGCCCCAGCAATAGCAGTGCCCCTTCAACCCTGCCCAGGTGGGCATTGGCGGACAgcgc                                                                                                                              <  2:187361/149‑1 (MQ=255)
                    cccGGCCCCGGACCTGGCGGCGTCCGGGGCAGGGTAGGTGCGTGCGTAATGGCGCGACTGATGCCAGAGCATTGCCAGGTAACCGGCCAGCCCCAGCAATAGCAGTGCCCCTTCAACCCTGCCCAGGTGGGCATTGGCGGACAGCGCAt                                                                                                                            <  5:33658/149‑1 (MQ=255)
                      cGGCCCCGGACCTGGCGGCGTCCGGGGCAGGGTAGGTGCGTGCGTAATGGCGCGACTGATGCCAGAGCATTGCCAGGTAACCGGCCAGGCCCAGCAATAGCAGTGCCCCTTCAACCGTGCCCACCTGGGCaggg                                                                                                                                         <  8:132991/134‑4 (MQ=255)
                      cGGCCCCGGACCTGGCGGCGTCCGGGGCAGGGTAGGTGCGTGCGTAATGGCGCGACTGATGCCAGAGCATTGCCAGGTAACCGGCCAGCCCCAGCAATAGCAGTGCCCCTTCAACCCTGCCCACCTGGGCaggg                                                                                                                                         >  7:132991/1‑131 (MQ=255)
                          cccGGACCTGGCGGCGTCCGGGGCAGGGTAGGTGCGTGCGTAATGGCGCGACTGATGCCAGAGCATTGCCAGGTAACCGGCCAGCCCCAGCAATAGCAGTGCCCCTTCAACCCTGCCCAGGTGGGCATTGGCGGACAGCGCATacac                                                                                                                        >  7:11939/1‑147 (MQ=255)
                                        cgTCCGGGGCAGGGTAGGTGCGTGCGTAATGGCGCGACTGATGCCAGAGCATTGCCAGGTAACCGGCCAGCCCCAGCAATAGCAGTGCCCCTTCAACCCTGCCCAGGTGGGCATTGGCGGACAGCGCATACACCAGCCCGCTGGCAAgg                                                                                                        <  2:153989/149‑1 (MQ=255)
                                          tCCGGGGCAGGGTAGGTGCGTGCGTAATGGCGCGACTGATGCCAGAGCATTGCCAGGTAACCGGCCAGCCCCAGCAATAGCAGTGCCCCTTCAACCCTGCCCAGGTGGGCATTGGCGGACAGCGCATACACCAGCCCGCTGGCAAGGat                                                                                                      >  3:111928/1‑149 (MQ=255)
                                                gCAGGGTAGGTGCGTGCGTAATGGCGCGACTGATGCCAGAGCATTGCCAGGTAACCGGCCAGCCCCAGCAATAGCAGTGCCCCTTCAACCCTGCCCAGGTGGGCATTGGCGGACAGCGCATACACCAGCCCGCTGGCAAGGATCATCAg                                                                                                <  8:116403/149‑1 (MQ=255)
                                                gCAGGGTAGGTGCGTGCGTAATGGCGCGACTGATGCCAGAGCATTGCCAGGTAACCGGCCAGCCCCAGCAATAGCAGTGCCCCTTCAACCCTGCCCAGGTGGGCATTGGCGGACAGCGCATACACCAGCCCGCTGGCAAGGATCATCAg                                                                                                <  4:111928/149‑1 (MQ=255)
                                                gCAGGGTAGGTGCGTGCGTAATGGCGCGACTGATGCCAGAGCATTGCCAGGTAACCGGCCAGCCCCAGCAATAGCAGTGCCCCTTCAACCCTGCCCAGGTGGGCATTGGCGGACAGCGCATACACCAGCCCGCTGGCAAGGATCATCAg                                                                                                >  1:229943/1‑149 (MQ=255)
                                                 cAGGGTAGGTGCGTGCGTAATGGCGCGACTGATGCCAGAGCATTGCCAGGTAACCGGCCAGCCCCAGCAATAGCAGTGCCCCTTCAACCCTGCCCAGGTGGGCATTGGCGGACAGCGCATACACCAGCCCGCTGGCAAGGATCATCAgg                                                                                               <  3:210731/149‑1 (MQ=255)
                                                          tgcgtgcgTAATGGCGCGACTGATGCCAGAGCATTGCCAGGTAACCGGCCAGCCCCAGCAATAGCAGTGCCCCTTCAACCCTGCCCAGGTGGGCATTGGCGGACAGCGCATACACCAGCCCGCTGGCAAGGATCATCAGGGGAATGTc                                                                                       >  8:13416/1‑148 (MQ=255)
                                                           gcgtgcgtAATGGCGCGACTGATGCCAGAGCATTGCCAGGTAACCGGCCAGCCCCAGCAATAGCAGTGCCCCTTCAACCCTGCCCAGGTGGGCATTGGCGGACAGCGCATACACCAGCCCGCTGGCAAGGATCATCAGGGGAATGTc                                                                                       >  5:205185/1‑147 (MQ=255)
                                                            cgtgcgtAATGGCGCGACTGATGCCAGAGCATTGCCAGGTAACCGGCCAGCCCCAGCAATAGCAGTGCCCCTTCAACCCTGCCCAGGTGGGCATTGGCGGACAGCGCATACACCAGCCCGCTGGCAAGGATCATCAGGGGAATGTCCAg                                                                                    <  8:11939/149‑1 (MQ=255)
                                                             gtgcgtAATGGCGCGACTGATGCCAGAGCATTGCCAGGTAACCGGCCAGCCCCAGCAATAGCAGTGCCCCTTCAACCCTGCCCAGGTGGGCATTGGCGGACAGCGCATACACCAGCCCGCTGGCAAGGATCATCAGGGGAATGTCCAGc                                                                                   >  7:139267/1‑149 (MQ=255)
                                                                              tGATGCCAGAGCATTGCCAGGTAACCGGCCAGCCCCAGCAATAGCTGTGCCCCTTCAACCCTGCCCAGGTGGGCATTGGCGGACAGCGCa                                                                                                                             <  2:215137/90‑1 (MQ=255)
                                                                              tGATGCCAGAGCATTGCCAGGTAACCGGCCAGCCCCAGCAATAGCTGTGCCCCTTCAACCCTGCCCAGGTGGGCATTGGCGGACAGCGCa                                                                                                                             >  1:215137/1‑90 (MQ=255)
                                                                                      gagCATTGCCAGGTAACCGGCCAGCCCCAGCAATAGCAGTGCCCCTTCAACCCTGCCCAGGTGGGCATTGGCGGACAGCGCATACACCAGCCCGCTGGCGAGGATCATCAGGGGAATGTCCAGCCGCACCAGCTGGCGTGACACCCg                                                            >  3:136991/1‑147 (MQ=255)
                                                                                         cATTGCCAGGTAACCGGCCAGCCCCAGCAATAGCAGTGCCCCTTCAACCCTGCCCAGGTGGGCATTGGCGGACAGCGCATACACCAGCCCGCTGGCAAGGATCATCAGGGGAATGTCCAGCCGCACCAGCTGGCGTGACACCCGCAGCg                                                       >  5:2273/1‑149 (MQ=255)
                                                                                           ttGCCAGGTAACCGGCCAGCCCCAGCAATAGCAGTGCCCCTTCAACCCTGCCCAGGTGGGCATTGGCGGACAGCGCATACACCAGCCCGCTGGCAAGGATCATCAGGGGAATGTCCAGCCGc                                                                                <  4:181796/122‑1 (MQ=255)
                                                                                           ttGCCAGGTAACCGGCCAGCCCCAGCAATAGCAGTGCCCCTTCAACCCTGCCCAGGTGGGCATTGGCGGACAGCGCATACACCAGCCCGCTGGCAAGGATCATCAGGGGAATGTCCAGCCGc                                                                                >  3:181796/1‑122 (MQ=255)
                                                                                                 ggTAACCGGCCAGCCCCAGCAATAGCAGTGCCCCTTCAACCCTGCCCAGGTGGGCATTGGCGGACAGCGCATACACCAGCCCGCTGGCAAGGATCATCAGCGGAATGTCCAGCCGCACCAGATGGCGTGACACCCGCAGCGGgatgat                                                >  2:97619/1‑148 (MQ=255)
                                                                                                      ccGGCCAGCCCCAGCAATAGCAGTGCCCCTTCAACCCTGCCCAGGTGGGCATTGGCGGACAGCGCATACACCAGCCCGCTGGCAAGGATCATCAGGGGAATGTCCAGCCGCACCAGCTGGCGTg                                                                   <  8:87621/124‑1 (MQ=255)
                                                                                                      ccGGCCAGCCCCAGCAATAGCAGTGCCCCTTCAACCCTGCCCAGGTGGGCATTGGCGGACAGCGCATACACCAGCCCGCTGGCAAGGATCATCAGGGGAATGTCCAGCCGCACCAGCTGGCGTg                                                                   >  7:87621/1‑124 (MQ=255)
                                                                                                       cGGCCAGCCCCAGCAATAGCAGTGCCCCTTCAACCCTGCCCAGGTGGGCATTGGCGGACAGCGCATACACCAGCCCGCTGGCAAGGATCATCAGGGGAATGTCCAGCCGCACCAGCTGGCGTGACACCCGCAGCGGGATGATCAGcgcg                                         >  3:153795/1‑149 (MQ=255)
                                                                                                               cccAGCAATAGCAGTGCCCCTTCAACCCTGCCCAGGTGGGCATTGGCGGACAGCGCATACACCAGCCCGCTGGCAAGGATCATCAGGGGAATGTCCAGCCGCACCAGCTGGCGTGACACCCGCAGCGGGATGATCAGCGCGGCCAGGcc                                 <  4:153795/149‑1 (MQ=255)
                                                                                                                ccAGCAATAGCAGTGCCCCTTCAACCCTGCCCAGGTGGGCATTGGCGGACAGCGCATACACCAGCCCGCTGGCAAGGATCATCAGGGGAATGTCCAGCCGCACCAGCTGGCGTGACACCCGCAGCGGGATGATCAGCGCGGCCAGGcc                                 >  6:189610/1‑148 (MQ=255)
                                                                                                                 cAGCAATAGCAGTGCCCCTTCAACCCTGCCCAGGTGGGCATTGGCGGACAGCGCATACACCAGCCCGCTGGCAAGGATCATCAGGGGAATGTCCAGCCGCACCAGCTGGCGTGACACCCGCAGCGGGATGATCAGCGCGGCCAGGCCga                               <  6:2273/149‑1 (MQ=255)
                                                                                                                 cAGCAATAGCAGTGCCCCTTCAACCCTGCCCAGGTGGGCATTGGCGGACAGCGCATACACCAGCCCGCTGGCAAGGATCATCAGGGGAATGTCCAGCCGCACCAGCTGGCGTGACACCCGCAGCGGGATGATCAGCGCGGCCAGGCCga                               <  2:229943/149‑1 (MQ=255)
                                                                                                                  atcAATAGCAGTGCCCCTTCAACCCTGCCCAGGTGGGCATTGGCGGACAGCGCATACACCAGCCCGCTGGCAAGGATCATCAGGGGAATGTCCAGCCGCACCAGCTGGCGTGACACCCGCAGCGGGATGATCAGCGCGGCCAGGCCgag                              <  6:205185/147‑1 (MQ=255)
                                                                                                                   gCAATAGCAGTGCCCCTTCAACCCTGCCCAGGTGGGCATTGGCGGACAGCGCATACACCAGCCCGCTGGCAAGGATCATCAGGGGAATGTCCAGCCGCACCAGCTGGCGTGACACCCGCAGCGGGATGATCAGCGCGGCCAGGCCgaga                             <  7:145948/149‑1 (MQ=255)
                                                                                                                     aaTAGGAGTGCCACTTCAACCCTGCCCAGGTGGGGATAGGCTGACATCGCAGAGACCAGCCCGCTGGCAAGGATGATCAGGGGAATGTCCAGCCGCACCAGCTGGCGTGACACCCGCAGCGGGATGATCAGCGCGGCCAGGCCGAGAAt                           <  8:88910/149‑1 (MQ=255)
                                                                                                                        aGCAGTGCCCCTTCAACCCTGCCCAGGTGGGCATTGGCGGACAGCGCATACACCAGCCCGCTGGCAAGGATCATCAGGGGAATGTCCAGCCGCACCAGCTGGCGTGACACCCGCAGCGGGATGATCAGCGCGGCCAGGCCGAGAATGAc                        <  7:252489/149‑1 (MQ=255)
                                                                                                                         gCAGTGCCCCTTCAACCCTGCCCAGGTGGGCATTGGCGGACAGCGCATACACCAGCCCGCTGGCAAGGATCATCAGGGGAATGTCCAGCCGCACCAGCTGGCGTGACACCCGCAGCGGGATGATCAGCGCGGCCAGGCCGAGAATGAc                        <  5:189610/148‑1 (MQ=255)
                                                                                                                          cAGTGCCCCTTCAACCCTGCCCAGGTGGGCATTGGCGGACAGCGCATACACCAGCCCGCTGGCAAGGATCATCAGGGGAATGTCCAGCCGCACCAGCTGGCGTGCCACCCGCAGCGGGATGATCAGCGCGGCCAGGCCGAGAATGAc                        >  7:218580/1‑147 (MQ=255)
                                                                                                                                      aaCCCTGCCCAGGTGGGCATTGGCGGACAGCGCATACACCAGCCCGCTGGCAAGGATCATCAGGGGAATGTCCAGCCGCACCAGCTGGCGTGACACCCGCAGCGGGATGATCAGCGCGGCCAGGCCGAGAATGACCAGTACGTTGAAg           <  2:148696/148‑1 (MQ=255)
                                                                                                                                       ccaatGCCCACCTGGGCATTGGCGGACAGCGCATACACCAGCCCGCTGGCAAGGATCATCAGGGGAATGTCCAGCCGCACCAGCTGGCGTGACACCCGCAGCTGGATGATCAGCGCGGCCAGGCCGAGAATGACCAGTACGTTGAAGa          >  1:230461/5‑148 (MQ=255)
                                                                                                                                        cccTGCCCAGGTGGGCATTGGCGGACAGCGCATACACCAGCCCGCTGGCAAGGATCATCAGGGGAATGTCCAGCCGCACCAGCTGGCGTGACACCCGCAGCGGGATGATCAGCGCGGCCAGGCCGAGAATGACCAGTACGTTGAAGATg        >  3:243468/1‑149 (MQ=255)
                                                                                                                                              ccAGGTGGGCATTGGCGGACAGCGCATACACCAGCCCGCTGGCAAGGATCATCAGGGGAATGTCCAGCCGCACCAGCTGGCGTGACACCCGCAGCGGGATGATCAGCGCGGCCAGGCCGAGAATGACCAGTACGTTGAAGATGTtgctg  <  4:243468/149‑1 (MQ=255)
                                                                                                                                                  gTGGGCATTGGCGGACAGCGCATACACCAGCCCGCTGGCAAGGATCATCAGGGGAATGTCCAGCCGCACCAGCTGGCGTGACACCCGCAGCGGGATGATCAGCGCGGCCAGGCCGAGAATGACCAGTACGTTGa             >  7:19435/1‑134 (MQ=255)
                                                                                                                                                  gTGGGCATTGGCGGACAGCGCATACACCAGCCCGCTGGCAAGGATCATCAGGGGAATGTCCAGCCGCACCAGCTGGCGTGACACCCGCAGCGGGATGATCAGCGCGGCCAGGCCGAGAATGACCAGTACGTTGa             <  8:19435/134‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
AGCGTACCTGACCAGAAGCGCCCGGCCCCGGACCTGGCGGCGTCCGGGGCAGGGTAGGTGCGTGCGTAATGGCGCGACTGATGCCAGAGCATTGCCAGGTAACCGGCCAGCCCCAGCAATAGCAGTGCCCCTTCAACCCTGCCCAGGTGGGCATTGGCGGACAGCGCATACACCAGCCCGCTGGCAAGGATCATCAGGGGAATGTCCAGCCGCACCAGCTGGCGTGACACCCGCAGCGGGATGATCAGCGCGGCCAGGCCGAGAATGACCAGTACGTTGAAGATGTTGCTG  >  NC_002947/1070892‑1071182

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑