Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,075,001 G→A 8.1% A60V (GCC→GTC)  gatC ← aspartyl/glutamyl‑tRNA(Asn/Gln) amidotransferase subunit C

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,075,0010GA 8.1% 140.8 / 2.4 49A60V (GCC→GTC) gatCaspartyl/glutamyl‑tRNA(Asn/Gln) amidotransferase subunit C
Reads supporting (aligned to +/- strand):  ref base G (20/25);  new base A (2/2);  total (22/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.45e-01

CATGGCAGGCTCTATCCCTTACTCGATGACTTTGGGAACCAGGTACAGACCGCTTTCGGTCGACGGCGCGATGGCCTGGTAGGCATCGCGCTGGTTGCTTTCGGTGACTTGGTCAGGACGCAGGCGCTGGCTGGCCTCCAGCGGGTGGGCCAGGGGCTCGATGCCAGTGGTATCGACCGCTTGCATCTGGTCGACCAGGCCGAGAATGCTGTTCAGGGCGTCGGTAATGCGTGGCAGTTCGCCATCATTCAGGCCCAGGCGGGCCAGATGGGCGATCTTTTCCAC  >  NC_002947/1074853‑1075137
                                                                                                                                                    |                                                                                                                                        
cATGGCAGGCTCTATCCCTTACTCGATGACTTTGGGAACCAGGTACAGACCGCTTTCGGTCGACGGCGCGATGGCCTGGTAGGCATCGCGCTGGTTGCTTTCGGTGACTTGGTCAGGACGCAGGCGCTGGCTGGCCTCCAGCGGGTggg                                                                                                                                          >  5:72586/1‑149 (MQ=255)
      aGGCTCTATCCCTTACTCGATGACTTTGGGAACCAGGTACAGACCGCTTTCGGTCGACGGCGCGATGGCCTGGTAGGCATCGCGCTGGTTGCTTTCGGTGACTTGGTCAGGACGCAGGCGCTGGCTGGCCTCCAGCGGGTGGGCCAggg                                                                                                                                    >  8:151496/1‑149 (MQ=255)
       ggCTCTATCCCTTACTCGATGACTTTGGGAACCAGGTACAGACCGCTTTCGGTCGACGGCGCGATGGCCTGGTAGGCATCGCGCTGGTTGCTTTCGGTGACTTGGTCAGGACGCAGGCGCTGGCTGGCCTCCAGCGGGTGGGCCAgggg                                                                                                                                   <  8:202895/149‑1 (MQ=255)
                ccTTACTCGATGACTTTGGGAACCAGGTACAGACCGCTTTCGGTCGACGGCGCGATGGCCTGGTAGGCATCGCGCTGGTTGCTTTCGGTGACTTGGTCAGGACGCAGGCGCTGGCTGGCCTCCAGCGGGTGGGCCAGGGGCTCGATGc                                                                                                                           <  4:79198/148‑1 (MQ=255)
                ccTTACTCGATGACTTTGGGAACCAGGTACAGACCGCTTTCGGTCGACGGCGCGATGGCCTGGTAGGCATCGCGCTGGTTGCTTTCGGTGACTTGGTCAGGACGCAGGCGCTGGCTGGCCTCCAGCGGGTGGGCCAGGGGCTCGATGc                                                                                                                           >  4:109637/1‑148 (MQ=255)
                     cTCGATGACTTTGGGAACCAGGTACAGACCGCTTTCGGTCGACGGCGCGATGGCCTGGTAGGCATCGCGCTGGTTGCTTTCGGTGACTTGGTCAGGACGCAGGCGCTGGCTGGCCTCCAGCGGGTGGGCCAGGGGCTCGATGCCAGTg                                                                                                                      <  2:80207/148‑1 (MQ=255)
                     cTCGATGACTTTGGGAACCAGGTACAGACCGCTTTCGGTCGACGGCGCGATGGCCTGGTAGGCATCGCGCTGGTTGCTTTCGGTGACTTGGTCAGGACGCAGGCGCTGGCTGGCCTCCAGCGGGTGGGCCAGGGGCTCGATGCCAGTg                                                                                                                      >  1:80207/1‑148 (MQ=255)
                     cTCGATGACTTTGGGAACCAGGTACAGACCGCTTTCGGTCGACGGCGCGATGGCCTGGTAGGCATCGCGCTGGTTGCTTTCGGTGACTTGGTCAGGACGCAGGCGCTGGCTGGCCTCCAGCGGGTGGGCCAGGGGCTCGATGCCAGTg                                                                                                                      <  1:64446/148‑1 (MQ=255)
                     cTCGATGACTTTGGGAACCAGGTACAGACCGCTTTCGGTCGACGGCGCGATGGCCTGGTAGGCATCGCGCTGGTTGCTTTCGGTGACTTGGTCAGGACGCAGGCGCTGGCTGGCCTCCAGCGGGTGGGCCAGGGGCTCGATGCCAGTg                                                                                                                      <  3:30086/148‑1 (MQ=255)
                     cTCGATGACTTTGGGAACCAGGTACAGACCGCTTTCGGTCGACGGCGCGATGGCCTGGTAGGCATCGCGCTGGTTGCTTTCGGTGACTTGGTCAGGACGCAGGCGCTGGCTGGCCTCCAGCGGGTGGGCCAGGGGCTCGATGCCAGTg                                                                                                                      >  4:30086/1‑148 (MQ=255)
                      tCGATGACTTTGGGAACCAGGTACAGACCGCTTTCGGTCGACGGCGCGATGGCCTGGTAGGCATCGCGCTGGTTGCTTTCGGTGACTTGGTCAGGACGCAGGCGCTGGCTGGCCTCCAGCGGGTGGGCCAGGGGCTCGATGCCAGTgg                                                                                                                     >  7:232676/1‑148 (MQ=255)
                        gATGACTTTGGGAACCAGGTACAGACCGCTTTCGGTCGACGGCGCGATGGCCTGGTAGGCATCGCGCTGGTTGCTTTCGGTGACTTGGTCAGGAAGCAGGCGCTGGCTGGCCACCAGCTCGTCGGCCAGGGCCTCGATGCCAGTGGTAt                                                                                                                  >  6:141757/1‑149 (MQ=255)
                         aTGACTTTGGGAACCATGTACAGACCGCTTTCGGTCGACGGCGCGATGGCCTGGTAGGCATCGCGCTGGTTGCTTTCGGTGACTTGGTCAGGACGCAGGCGCTGGCTGGCCTCCAGCGGGTGGGCCAGGGGCt                                                                                                                                 <  6:117483/133‑1 (MQ=255)
                         aTGACTTTGGGAACCAGGTACAGACCGCTTTCGGTCGACGGCGCGATGGCCTGGTAGGCATCGCGCTGGTTGCTTTCGGTGACTTGGTCAGGACGCAGGCGCTGGCTGGCCTCCAGCGGGTGGGCCAGGGGCt                                                                                                                                 >  5:117483/1‑133 (MQ=255)
                             cTTTGGGAACCAGGTACAGACCGCTTTCGGTCGACGGCGCGATGGCCTGGTAGGCATCGCGCTGGTTGCTTTCGGTGACTTGGTCAGGACGCAGGCGCTGGCTGGCCTCCAGCGGGTGGGCCAGGGGCTCGATACCACTGGCATCGagc                                                                                                             <  5:113336/149‑3 (MQ=255)
                                  ggAACCAGGTACAGACCGCTTTCGGTCGACGGCGCGATGGCCTGGTAGGCATCGCGCTGGTTGCTTTCGGTGACTTGGTCAGGACGCAGGCGCTGGCTGGCCTCCAGCGGGTGGGCCAGGGGCTCGATGCCAGTGGTATCGACCGCtt                                                                                                         <  8:232676/148‑1 (MQ=255)
                                  ggAACCAGGTACAGACCGCTTTCGGTCGACGGCGCGATGGCCTGGTAGGCATCGCGCTGGTTGCTTTCGGTGACTTGGTCAGGACGCAGGCGCTGGCTGGCCTCCAGCGGGTGGGCCAGGGGCTCGATGCCAGTGGTATCGACCGCTTg                                                                                                        <  7:151496/149‑1 (MQ=255)
                                      ccAGGTACAGACCGCTTTCGGTCGACGGCGCGAAGGCCTGGTAGGCATCGCGCTGGTTGCTTTCGGTGACTTGGTCAGGACGCAGGCGCTGGCTGGCCTCCAGCGGGTGGGCCAGGGGCTCGATGCCAGTGGTATCGACCGCTTGCATc                                                                                                    >  4:14939/1‑149 (MQ=255)
                                        aGGTACAGACCGCTTTCGGTCGACGGCGCGAAGGCCTGGTAGGCATCGCGCTGGTTGCTTTCGGTGACTTGGTCAGGACGCAGGCGCTGGCTGGCCTCCAGCGGGTGGGCCAGGGGCTCGATGCCAGTGGTATCGACCGCTTGCATCTg                                                                                                  <  3:14939/149‑1 (MQ=255)
                                         ggTACAGACCGCTTTCGGTCGACGGCGCGATGGCCTGGTAGGCATCGCGCTGGTTGCTTTCGGTGACTTGGTCAGGACGCAGGCGCTGGCTGGCCTCCAGCGGGTGGGCCAGGGGCTCGa                                                                                                                              <  8:103577/120‑1 (MQ=255)
                                         ggTACAGACCGCTTTCGGTCGACGGCGCGATGGCCTGGTAGGCATCGCGCTGGTTGCTTTCGGTGACTTGGTCAGGACGCAGGCGCTGGCTGGCCTCCAGCGGGTGGGCCAGGGGCTCGa                                                                                                                              >  7:103577/1‑120 (MQ=255)
                                         ggTACAGACCGCTTTCGGTCGACGGCGCGATGGCCTGGTAGGCATCGCGCTGGTTGCTTTCGGTGACTTGGTCAGGACGCAGGCGCTGGCTGGCCTCCAGCGGGTGGGCCAGGGGCTCGATGCCAGTGGTATCGACCGCTTGCATCTgg                                                                                                 <  8:251428/149‑1 (MQ=255)
                                             cAGACCGCTTTCGGTCGACGGCGCGATGGCCTGGTTGGCATCGCGCTGGTTGCTTTCGGTGACTTGGTCAGGACGCAGGCGCTGGCTGGCCTCCAGCGGGTGGACCAGGGGCTCGATGCCAGTGGTATCGACCGCTTGCATCTGGTCGa                                                                                             >  7:159651/1‑149 (MQ=255)
                                             cAGACCGCTTTCGGTCGACGGCGCGATGGCCTGGTTGGCATCGCGCTGGTTGCTTTCGGTGACTTGGTCAGGACGCAGGCGCTGGCTGGCCTCCAGCGGGTGGACCAGGGGCTCGATGCCAGTGGTATCGACCGCTTGCATCTGGTCGa                                                                                             >  7:163702/1‑149 (MQ=255)
                                                  cGCTTTCGGTCGACGGCGCGATGGCCTGGTAGGCATCGCGCTGGTTGCTTTCGGTGACTTGGTCAGGACGCAGGCGCTGGCTGGCCTCCAGCGGGTGGGCCAGGGGCTCGATGCCAGTGGTATCGACCGCTTGCATCTGGTCGACCAgg                                                                                        <  2:89883/149‑1 (MQ=255)
                                                  cGCTTTCGGTCGACGGCGCGATGGCCTGGTAGGCATCGCGCTGGTTGCTTTCGGTGACTTGGTCAGGACGCAGGCGCTGGCTGGCCTCCAGCGGGTGGGCCAGGGGCTCGATGCCAGTGGTATCGACCGCTTGCATCTGGTCGACCAgg                                                                                        <  4:2638/149‑1 (MQ=255)
                                                         ggTCGACGGCGCGATGGCCTGGTTGGCATCGCGCTGGTTGCTTTCGGTGACTTGGTCAGGACGCAGGCGCTGGCTGGCCTCCAGCGGGTGGACCAGGGGCTCGATGCCAGTGGTATCGACCGCTTGCATCTGGTCGACCAGGCCgaga                                                                                  <  8:159651/148‑1 (MQ=255)
                                                         ggTCGACGGCGCGATGGCCTGGTTGGCATCGCGCTGGTTGCTTTCGGTGACTTGGTCAGGACGCAGGCGCTGGCTGGCCTCCAGCGGGTGGACCAGGGGCTCGATGCCAGTGGTATCGACCGCTTGCATCTGGTCGACCAGGCCgaga                                                                                  <  8:163702/148‑1 (MQ=255)
                                                         ggTCGACGGCGCGATGGCCTGGTAGGCATCGCGCTGGTTGCTTTCGGTGACTTGGTCAGGACGCAGGCGCTGGCTGGCCTCCAGCGGGTGGGCCAGGGGCTCGATGCCAGTGGTATCGACCGCTTGCATCTGGTCGACCAGGCCGAGaa                                                                                 <  7:56600/149‑1 (MQ=255)
                                                           tCGACGGCGCGATGGCCTGGTAGGCATCGCGCTGGTTGCTTTCGGTGACTTGGTCAGGACGCAGGCGCTGGCTGGCCTCCAGCGGGTGGGCCAGGGGCTCGATGCCAGTGGTATCGACCGCGTGCATCTGGTCGACCAGGCCGAGAATg                                                                               >  6:259503/1‑149 (MQ=255)
                                                             gACGGCGCGATGGCCTGGTAGGCATCGCGCTGGTTGCTTTCGGTGACTTGGTCAGGACGCAGGCGCTGGCTGGCCTCCAGCGGGTGGGCCAGGGGCTCGATGCCAGTGGTATCGACCGCTTGCATCTGGTCGACCAGGCCGAGAATGCt                                                                             <  3:109637/149‑1 (MQ=255)
                                                                      aTGGCCTGGTAGGCATCGCGCTGGTTGCTTTCGGTGACTTGGTCAGGACGCAGGCGCTGGCTGGCCTCCAGCGGGTGGGCCAGGGGCTCGATGCCAGTGGTATCGACCGCTTGCATCTGGTCGACCAGGCCGAGAATGCTGTTCAGGGc                                                                    <  2:188234/149‑1 (MQ=255)
                                                                          ccTGGTAGGCATCGCGCTGGTTGCTTTCGGTGACTTGGTCAGGACGCAGGCGCTGGCTGGCCTCCAGCGGGTGGGCCAGGGGCTCGATGCCAGTGGTATCGACCGCTTGCATCTGGTCGACCAGGCCGAGAATGCTGTTCAGGGCGTCg                                                                >  6:41397/1‑149 (MQ=255)
                                                                            tGGTAGGCATCGCGCTGGTTGCTTTCGGTGACTTGGTCAGGACGCAGGCGCTGGCTGGCCTCCAGCGGGTGGGCCAGGGGCTCGATGCCAGTGGTATCGACCGCTTGCATCTGGTCGACCAGGCCGAGAATGCTGTTCAGGGCGTCgg                                                               >  1:184669/1‑148 (MQ=255)
                                                                                     tCGCGCTGGTTGCTTTCGGTGACTTGGTCAGGACGCAGGCGCTGGCTGGCCTCCAGCGGGTGGGCCAGGGGCTCGATGCCAGTGGTATCGACCGCTTGCATCTGGTCGACCAGGCCGAGAATGCTGTTCAGGGCGTCGGTAATGCGTgg                                                     <  6:111071/149‑1 (MQ=255)
                                                                                             gTTGCTTTCGGTGACTTGGTCAGGACGCAGGCGCTGGCTGGCCTCCAGCGGGTGGGCCAGGGGCTCGATGCCAGTGGTATCGACCGCTTGCATCTGGTCGACCAGGCCGAGAATGCTGTTCAGGGCGTCGGTAATGCGTGGCAGTTCGc                                             <  6:72586/149‑1 (MQ=255)
                                                                                             gTTGCTTTCGGTGACTTGGTCAGGACGCAGGCGCTGGCTGGCCTCCAGCGGGTGGGCCAGGGGCTCGATGCCAGTGGTATCGACCGCTTGCATCTGGTCGACCAGGCCGAGAATGCTGTTCAGGGCGTCGGTAATGCGTGGCAGTTCGc                                             >  7:60870/1‑149 (MQ=255)
                                                                                                        tGACTTGGTCAGGACGCAGGCGCTGGCTGGCCTCCAGCGGGTGGGCCAGGGGCTCGATGCCAGTGGTATCGACCGCTTGCATCTGGTCGACCAGGCCGAGAATGCTGTTCAGGGCGTCGGTAATGCGTGGCAGTTCGCCATCATTCAgg                                  <  5:41397/149‑1 (MQ=255)
                                                                                                            ttGGTCAGGACGCAGGCGCTGGCTGGCCTCCAGCGGGTGGGCCAGGGGCTCGATGCCAGTGGTATCGACCGCTTGCATCTGGTCGACCAGGCCGAGAATGCTGTTCAGGGCGTCGGTAATGCGTGGCAGTTCGCCATCATTCAGGcccg                              >  6:265092/1‑148 (MQ=255)
                                                                                                            ttGGTCAGGACGCAGGCGCTGGCTGGCCTCCAGCGGGTGGGCCAGGGGCTCGATGCCAGTGGTATCGACCGCTTGCATCTGGTCGACCAGGCCGAGAATGCTGTTCAGGGCGTCGGTAATGCGTGGCAGTTCGCCATCATTCAGGcc                                >  6:226284/1‑147 (MQ=255)
                                                                                                            ttGGTCAGGACGCAGGCGCTGGCTGGCCTCCAGCGGGTGGGCCAGGGGCTCGATGCCAGTGGTATCGACCGCTTGCATCTGGTCGACCAGGCCGAGAATGCTGTTCAGGGCGTCGGTAATGCGTGGCAGTTCGCCATCATTCAGGcc                                >  8:16261/1‑147 (MQ=255)
                                                                                                                       gCAGGCGCTGGCTGGCCTCCAGCGGGTGGGCCAGGGGCTCGATGCCAGTGGTATCGACCGCTTGCATCTGGTCGACCAGGCCGAGAATGCTGTTCAGGGCGTCGGTAATGCGTGGCAGTTCGCCATCATTCAGGCCCAGGCGGGc                       >  4:181439/1‑145 (MQ=255)
                                                                                                                       gCAGGCGCTGGCTGGCCTCCAGCGGGTGGGCCAGGGGCTCGATGCCAGTGGTATCGACCGCTTGCATCTGGTCGACCAGGCCGAGAATGCTGTTCAGGGCGTCGGTAATGCGTGGCAGTTCGCCATCATTCAGGCCCAGGCGGGCCAGa                   <  3:181439/149‑1 (MQ=255)
                                                                                                                                 gctggcCTCCAGCGGGTGGGCCAGGGGCTCGATGCCAGTGGTATCGACCGCTTGCATCTGGTCGACCAGGCCGAGAATGCTGTTCAGGGCGTCGGTAATGCGTGGCAGTTCGCCATCATTCAGGCCCAGGCGGGCCAGATGGGCGATc          <  1:262629/148‑1 (MQ=255)
                                                                                                                                   tggcCTCCAGCGGGTGGGCCAGGGGCTCGATGCCAGTGGTATCGACCGCTTGCATCTGGTCGACCAGGCCGAGAATGCTGTTCAGGGCGTCGGTAATGCGTGGCAGTTCGCCATCATTCAGGCCCAGGCGGGCCAGATGGGCGATCttt       >  7:239853/1‑149 (MQ=255)
                                                                                                                                     gcCTCCAGCGGGTGGGCCCGGGGCTCGATGCCAGTGGTATCGACCGCTTGCATCTGGGCGCCCAGGCCGAGAAGGCTGTTCAGGGCGTCGGTAATGCGGGGCAGTTCGCCATCATTCAGGCCCAGGCGGGCCAGATGGGCGATCTTTTc     <  5:259503/149‑1 (MQ=255)
                                                                                                                                     gcCTCCAGCGGGTGGGCCAGGGGCTCGATGCCAGTGGTATCGACCGCTTGCATCTGGTCGACCAGGCCGAGAATGCTGTTCAGGGCGTCGGTAATGCGTGGCAGTTCGCCATCATTCAGTCCCAGGCGGGCCAGATGGGCGATCTTTTc     <  5:141757/149‑1 (MQ=255)
                                                                                                                                     gcCTCCAGCGGGTGGGCCAGGGGCTCGATGCCAGTGGTATCGACCGCTTGCATCTGGTCGACCAGGCCGAGAATGCTGTTCAGGGCGTCGGTAATGCGTGGCAGTTCGCCATCATTCAGGCCCAGGCGGGCCAGATGGGCGATCTTTTc     <  5:226284/149‑1 (MQ=255)
                                                                                                                                       cTCCAGCGGGTGGGCCAGGGGCTCGATGCCAGTGGTATCGACCGCTTGCATCTGGTCGACCAGGCCGAGAATGCTGTTCAGGGCGTCGGTAATGCGTGGCAGTTCGCCATCATTCAGGCCCAGGCGGGCCAGATGGGCGATCTTTTCCa   >  7:252045/1‑149 (MQ=255)
                                                                                                                                        tCCAGCGGGTGGGCCAGGGGCTCGATGCCAGTGGTATCGACCGCTTGCATCTGGTCGACCAGGCCGAGAATGCTGTTCAGGGCGTCGGTAATGCGTGGCAGTTCGCCATCATTCAGGCCCAGGCGGGCCAGATGGGCGATCTTTTCCAc  >  8:74212/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                        
CATGGCAGGCTCTATCCCTTACTCGATGACTTTGGGAACCAGGTACAGACCGCTTTCGGTCGACGGCGCGATGGCCTGGTAGGCATCGCGCTGGTTGCTTTCGGTGACTTGGTCAGGACGCAGGCGCTGGCTGGCCTCCAGCGGGTGGGCCAGGGGCTCGATGCCAGTGGTATCGACCGCTTGCATCTGGTCGACCAGGCCGAGAATGCTGTTCAGGGCGTCGGTAATGCGTGGCAGTTCGCCATCATTCAGGCCCAGGCGGGCCAGATGGGCGATCTTTTCCAC  >  NC_002947/1074853‑1075137

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑