Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,178,753 A→C 8.2% intergenic (+58/+36) guaA → / ← PP_1033 glutamine‑hydrolyzing GMP synthase/sulfatase domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,178,7530AC 8.2% 138.1 / 3.7 49intergenic (+58/+36)guaA/PP_1033glutamine‑hydrolyzing GMP synthase/sulfatase domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base A (22/22);  new base C (2/2);  total (24/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.73e-01

CAGCGGTCGTATCATCAACGAAATCGACGGTATCTCGCGCGTTACCTACGACGTGTCGAGCAAGCCGCCTGCCACTATCGAGTGGGAGTGATCGGGTAGCCAGCCCAGGCTGGCACCCGCAGGACGGATACACCAGAAAGCCCGCATGATTGCGGGCTTTCTGGTTCTTGTTCGTTCAGATTGCTTATTCTTCTGGCTTTCTGGCAAATAGCGCTTTCTGGTAGGCGTTATAGGAAAGCAGCCTGCTCCTCACTGCGATGAACTCGTCACCCTCCACGCGTTGGGGGTGCTTTT  >  NC_002947/1178605‑1178898
                                                                                                                                                    |                                                                                                                                                 
cAGCGGTCGTATCATCAACGAAATCGACGGTATCTCGCGCGTTACCTACGACGTGTCGAGCAAGCCGCCTGCCACTATCGAGTGGGAGTGATCGGGTAGCCAGCCCAGGCTGGCACCCGCAGGACGGATACACCAGAAAGCCCGCATGa                                                                                                                                                   >  7:3410/1‑149 (MQ=255)
     tgcGTATCTTCCACGACTTCGACGGGAGCTGGCGCGTGACCTACGACGTGTCGAGCAAGCCGCCTGCCACTATCGAGTGGGAGTGATCGGGTAGCCAGCCCAGGCTGGCACCCGCAGGACGGATACACCAGAAAGCCCGCATGATTGCg                                                                                                                                              <  4:186442/147‑1 (MQ=255)
     gTCGTATCATGAACGTATTCGACGGTATCTCGCGCGTTACCTACGTCGTGTCGAGCAAGCCGCCTGCCACTATCGAGTGGGAGTGATCGGGTAGCCAGCCCAGGCTGGCACCCGCAGGACGGATACACCAGAAAGCCCGCATGATTGCg                                                                                                                                              <  4:189877/149‑1 (MQ=255)
        gTATCATCAACGAAATCGACGGTATCTCGCGCGTTACCTACGACGTGTCGAGCAAGCCGCCTGCCACTATCGAGTGGGAGTGATCGGGTAGCCAGCCCAGGCTGGCACCCGCAGGACGGATACACCAGAAAGCCCGCATGATTGCggg                                                                                                                                            <  4:234241/148‑1 (MQ=255)
        gTATCATCAACGAAATCGACGGTATCTCGCGCGTTACCTACGACGTGTCGAGCAAGCCGCCTGCCACTATCGAGTGGGAGTGATCGGGTAGCCAGCCCAGGCTGGCACCCGCAGGACGGATACACCAGAAAGCCCGCATGATTGCGGGc                                                                                                                                           <  6:227188/149‑1 (MQ=255)
             atcaACGAAATCGACGGTATCTCGCGCGTTACCTACGACGTGTCGAGCAAGCCGCCTGCCACTATCGAGTGGGAGTGATCGGGTAGCCAGCCCAGGCTGGCACCCGCAGGACGGATACACCAGAAAGCCCGCATGATTGCGGGCTTTCt                                                                                                                                      >  4:209401/1‑149 (MQ=255)
             atcaACGAAATCGACGGTATCTCGCGCGTTACCTACGACGTGTCGAGCAAGCCGCCTGCCACTATCGAGTGGGAGTGATCGGGTAGCCAGCCCAGGCTGGCACCCGCAGGACGGATACACCAGAAAGCCCGCATGATTGCGGGCTTTCt                                                                                                                                      <  3:209401/149‑1 (MQ=255)
              tcaACGAAATCGACGGTATCTCGCGCGTTACCTACGACGTGTCGAGCAAGCCGCCTGCCACTATCGAGTGGGAGTGATCGGGTAGCCAGCCCAGGCTGGCACCCGCAGGACGGATACACCAGAAAGCCCGCAATCATGCGGGCTTTCt                                                                                                                                      <  7:246907/148‑1 (MQ=255)
              tcaACGAAATCGACGGTATCTCGCGCGTTACCTACGACGTGTCGAGCAAGCCGCCTGCCACTATCGAGTGGGAGTGATCGGGTAGCCAGCCCAGGCTGGCACCCGCAGGACGGATACACCAGAAAGCCCGCAATCATGCGGGCTTTCt                                                                                                                                      >  8:246907/1‑148 (MQ=255)
                aaCGAAATCGACGGTATCTCGCGCGTTACCTACGACGTGTCGAGCAAGCCGCCTGCCACTATCGAGTGGGAGTGATCGGGTAGCCAGCCCAGGCTGGCACCCGCAGGACGGATACACCAGAAAGCCCGCATGATTGCGGGCTTTCTGGt                                                                                                                                   >  7:201953/1‑149 (MQ=255)
                 aCGAAATCGACGGTATCTCGCGCGTTACCTACGACGTGTCGAGCAAGCCGCCTGCCACTATCGAGTGGGAGTGATCGGGTAGCCAGCCCAGGCTGGCACCCGCAGGACGGATACACCAGAAAGCCCGCATGATTGCGGGCTTTCTGGtt                                                                                                                                  >  5:55634/1‑149 (MQ=255)
                 aCGAAATCGACGGTATCTCGCGCGTTACCTACGACGTGTCGAGCAAGCCGCCTGCCACTATCGAGTGGGAGTGATCGGGTAGCCAGCCCAGGCTGGCACCCGCAGGACGGATACACCAGAAAGCCCGCATGATTGCGGGCTTTCTGGtt                                                                                                                                  >  8:97705/1‑149 (MQ=255)
                   gAAATCGACGGTATCTCGCGCGTTACCTACGACGTGTCGAGCAAGACGCCTGCCACTATCGAGTGGGAGTGATCGGGTAGCCAGCCCAGGCTGGCACCCGCAGGACGGATACACCAGAAAGCCCGCATCATTTCGGGCTTTCTGGATCt                                                                                                                                >  6:149877/1‑149 (MQ=255)
                      aTCGACGGTATCTCGCGCGTTACCTACGACGTGTCGAGCAAGCCGCCTGCCACTATCGAGTGGGAGTGATCGGGTAGCCAGCCCAGGCTGGCACCCGCAGGACGGATACACCAGAAAGCCCGCATGATTGCGGGCTTTCTGGTTCTTgt                                                                                                                             >  5:250763/1‑149 (MQ=255)
                      aTCGACGGTATCTCGCGCGTTACCTACGACGTGTCGAGCAAGCCGCCTGCCACTATCGAGTGGGAGTGATCGGGTAGCCAGCCCAGGCTGGCACCCGCAGGACGGATACACCAGAAAGCCCGCATGATTGCGGGCTTTCTGGTTCTTgt                                                                                                                             >  6:148057/1‑149 (MQ=255)
                         gACGGTATCTCGCGCGTTACCTACGACGTGTCGAGCAAGCCGCCTGCCACTATCGAGTGGGAGTGATCGGGTAGCCAGCCCAGGCTGGCACCCGCAGGACGGATACACCAGAAAGCCCGCATGATTGCGGGCTTTCTGGTTCTTgttc                                                                                                                           <  6:55634/148‑1 (MQ=255)
                         gACGGTATCTCGCGCGTTACCTACGACGTGTCGAGCAAGCCGCCTGCCACTATCGAGTGGGAGTGATCGGGTAGCCAGCCCAGGCTGGCACCCGCAGGACGGATACACCAGAAAGCCCGCATGATTGCGGGCTTTCTGGTTCTTgttc                                                                                                                           <  5:74962/148‑1 (MQ=255)
                          aCGGTATCTCGCGCGTTACCTACGACGTGTCGAGCAAGCCGCCTGCCACTATCGAGTGGGAGTGATCGGGTAGCCAGCCCAGGCTGGCACCCGCAGGACGGATACACCAGAAAGCCCGCATGATTGCGGGCTTTCTGGTTCTTgttcgt                                                                                                                         >  6:48641/1‑149 (MQ=255)
                                tctcGCGCGTTACCTACGACGTGTCGAGCAAGCCGCCTGCCACTATCGAGTGGGAGTGATCGGGTAGCCAGCCCAGGCTGGCACCCGCAGGACGGATACACCAGAAAGCCCGCATGATTGCGGGCTTTCTGGTTCTTGTTCGTTCAGa                                                                                                                    <  2:176489/148‑1 (MQ=255)
                                   cgcgcgTTACCTACGACGTGTCGAGCAAGCCGCCTGCCACTATCGAGTGGGAGTGATCGGGTAGCCAGCCCAGGCTGGCACCCGCAGGACGGATACACCAGAAAGCCCGCATGATTGCGGGCTTTCTGGTTCTTGTTCGTTCAGAt                                                                                                                   <  2:108472/146‑1 (MQ=255)
                                   cgcgcgTTACCTACGACGTGTCGAGCAAGCCGCCTGCCACTATCGAGTGGGAGTGATCGGGTAGCCAGCCCAGGCTGGCACCCGCAGGACGGATACACCAGAAAGCCCGCATGATTGCGGGCTTTCTGGTTCTTGTTCGTTCAGAt                                                                                                                   >  1:108472/1‑146 (MQ=255)
                                    gcgcgTTACCTACGACGTGTCGAGCAAGCCGCCTGCCACTATCGAGTGGGAGTGATCGGGTAGCCAGCCCAGGCTGGCACCCGCAGGACGGATACACCAGAAAGCCCGCATGATTGCGGGCTTTCTGGTTCTTGTTCGTTCAGATTGc                                                                                                                <  5:48641/148‑1 (MQ=255)
                                       cgTTACCTACGACGTGTCGAGCAAGCCGCCTGCCACTATCGAGTGGGAGTGATCGGGTAGCCAGCCCAGGCTGGCACCCGCAGGACGGATACACCAGAAAGCCCGCATGATTGCGGGCTTTCTGGTTCTTGTTCGTTCAGATTGCTTAt                                                                                                            >  1:208139/1‑149 (MQ=255)
                                                cgacgTGTCGAGCAAGCCGCCTGCCACTATCGAGTGGGAGTGATCGGGTAGCCAGCCCAGGCTGGCACCCGCAGGACGGATACACCAGAAAGCCCGCATGATTGCGGGCTTTCTGGTTCTTGTTCGTTCAGATTGCTTATTCTTCTg                                                                                                     <  6:250763/147‑1 (MQ=255)
                                                 gacgTGTCGAGCAAGCCGCCTGCCACTATCGAGTGGGAGTGATCGGATAGCCAGCCCAGGCTGGCACCCGCAGGACGGATACACCAGAAAGCCCGCATGATTGCGGGCTTTCTGGTTCTTGTTCGTTCAGATTGCTTATtcttct                                                                                                      >  6:211188/1‑145 (MQ=255)
                                                 gacgTGTCGAGCAAGCCGCCTGCCACTATCGAGTGGGAGTGATCGGATAGCCAGCCCAGGCTGGCACCCGCAGGACGGATACACCAGAAAGCCCGCATGATTGCGGGCTTTCTGGTTCTTGTTCGTTCAGATTGCTTATtcttct                                                                                                      <  5:211188/145‑1 (MQ=255)
                                                    gtgtCGAGCAAGCCGCCTGCCACTATCGAGTGGGAGTGATCGGGTAGCCAGCCCAGGCTGGCACCCGCAGGACGGATACACCAGAAAGCCCGCATGATTGCGGGCTTTCTGGTTCTTGTTCGTTCAGATTGCTTATTCTTCTGGCTTTc                                                                                               <  7:15646/149‑1 (MQ=255)
                                                     tgtCGAGCAAGCCGCCTGCCACTATCGAGTGTGAGTGATCGGGTAGCGAGCCCAGGCTGGCACCCGCATGACGGATACACCAGAAAGCCCGCATGTTTGCGGGCTTTCTGGTTCTTGTTCGTTCAGATTGCTTATTCTTCTGGCTTTCt                                                                                              <  5:149877/149‑1 (MQ=255)
                                                        cGAGCAAGCCGCCTGCCACTATCGAGTGGGAGTGATCGGGTAGCCAGCCCAGGCTGGCACCCGCAGGACGGATACACCAGAAAGCCCGCATGATTGCGGGCTTTCTGGTTCTTGTTCGTTCAGATTGCTTATTCTTCTGGCTTTCTgg                                                                                            >  5:186705/1‑148 (MQ=255)
                                                                       ccACTATCGAGTGGGAGTGATCGGGTAGCCAGCCCAGGCTGGCACCCGCAGGACGGATACGCCAGAAAGCCCGCATGATTGCGGGCTTTCTGGTTCTTGTTCGTTCAGATTGCTTATTCTTCTGGCTTTCTGGCAAATAGCGCTTTc                                                                              <  4:251619/147‑1 (MQ=255)
                                                                             tCGAGTGGGAGTGATCGGGTAGCCAGCCCAGGCTGGCACCCGCAGGACGGATACACCAGAAAGCCCGCATGATTGCGGGCTTTCTGGTTCTTGTTCGTTCAGATTGCTTATTCTTCTGGCTTTCTGGCAAATAGCGCTTTCTGGTAGGc                                                                      <  5:148057/149‑1 (MQ=255)
                                                                                aGTGGGAGTGATCGGGTAGCCAGCCCAGGCTGGCACCCGCAGGACGGATACACCAGAAAGCCCGCATGATTGCGGGCTTTCTGGTTCTTGTTCGTTCAGATTGCTTATTCTTCTGGCTTTCTGGCAAATAGCGCTTTCTGGTAGGCGtt                                                                   >  8:157779/1‑149 (MQ=255)
                                                                                 gttggAGTGATCGGGTAGCCAGCCCAGGCTGGCACCCGCAGGACGGATACACCAGAAAGCCCGCATGATTGCGGGCTTTCTGGTTCTTGTTCGTTCAGATTGCTTATTCTTCTGGCTTTCTGGCAAATAGCGCTTTCTGGTa                                                                         <  3:159965/139‑1 (MQ=255)
                                                                                 gTGGGAGTGATCGGGTAGCCAGCCCAGGCTGGCACCCGCAGGACGGATACACCAGAAAGCCCGCATGATTGCGGGCTTTCTGGTTCTTGTTCGTTAAGATTGCTTATTCTTCTGGCTTTCTGGCAAATAGCGCTTTCTGGTa                                                                         >  4:159965/1‑142 (MQ=255)
                                                                                  tGGGAGTGATCGGGTAGCCAGCCCAGGCTGGCACCCGCAGGACGGATACACCAGAAAGCCCGCATGATTGCGGGCTTTCTGGTTCTTGTTCGTTCAGATTGCTTATTCTTCTGGCTTTCTGGCAAATAGCGCTTTCTGGTAGGCGTtat                                                                 <  2:160786/149‑1 (MQ=255)
                                                                                             gggTAGCCAGCCCAGGCTGGCACCCGCAGGACGGATACACCAGAAAGCCCGCATGATTGCGGGCTTTCTGGTTCTTGTTCGTTCAGATTGCTTATTCTTCTGGCTTTCTGGCAAATAGCGCTTTCTGGTAGGCGTTATAGGAAagcagc                                                      <  2:208139/149‑1 (MQ=255)
                                                                                                  gccagccCAGGCTGGCACCCGCAGGACGGATACACCAGAAAGCCCGCATGATTGCGGGCTTTCTGGTTCTTGTTCGTTCAGATTGCTTATTCTTCTGGCTTTCTGGCAAATAGCGCTTTCTGGTAGGCGTTATAGGAAAGCAGCCTGct                                                 >  1:163891/1‑149 (MQ=255)
                                                                                                                                   acCAGAAAGCCCGCATGATTGCGGGCTTTCTGGTTCTTGTTCGTTCAGATTGCTTATTCTTCTGGCTTTCTGGCAAATAGCGCTTTCTGGTAGGCGTTATAGGAAAGCAGCCTGCTCCTCACTGCGATGAACTCGTCACCCTc                      >  4:204600/1‑143 (MQ=255)
                                                                                                                                   acCAGAAAGCCCGCATGATTGCGGGCTTTCTGGTTCTTGTTCGTTCAGATTGCTTATTCTTCTGGCTTTCTGGCAAATAGCGCTTTCTGGTAGGCGTTATAGGAAAGCAGCCTGCTCCTCACTGCGATGAACTCGTCACCCTc                      <  3:204600/143‑1 (MQ=255)
                                                                                                                                   acCAGAAAGCCCGCATGATTGCGGGCTTTCTGGTTCTTGTTCGTTCAGATTGCTTATTCTTCTGGCTTTCTGGCAAATAGCGCTTTCTGGTAGGCGTTATAGGAAAGCAGCCTGCTCCTCACTGCGATGAACTCGTCACCCTCCAcgcg                >  7:35000/1‑149 (MQ=255)
                                                                                                                                   acCAGAAAGCCCGCATGATTGCGGGCTTTCTGGTTCTTGTTCGTTCAGATTGCTTATTCTTCTGGCTTTCTGGCAAATAGCGCTTTCTGGTAGGCGTTATAGGAAAGCAGCCTGCTCCTCACTGCGATGAACTCGTCACCCTCCAcgcg                >  1:122697/1‑149 (MQ=255)
                                                                                                                                     cAGAAAGCCCGCATGATTGCGGGCTTTCTGGTTCTTGTTCGTTCAGATTGCTTATTCTTCTGGCTTTCTGGCAAATAGCGCTTTCTGGTAGGCGTTATAGGAAAGCAGCCTGCTCCTCACTg                                         <  6:85539/122‑1 (MQ=255)
                                                                                                                                     cAGAAAGCCCGCATGATTGCGGGCTTTCTGGTTCTTGTTCGTTCAGATTGCTTATTCTTCTGGCTTTCTGGCAAATAGCGCTTTCTGGTAGGCGTTATAGGAAAGCAGCCTGCTCCTCACTg                                         >  5:85539/1‑122 (MQ=255)
                                                                                                                                     cAGAAAGCCCGCATGATTGCGGGCTTTCTGGTTCTTGTTCGTTCAGATTGCTTATTCTTCTGGCTTTCTGGCAAATAGCGCTTTCTGGTAGGCGTTATAGGAAAGCAGCCTGCTCCTCACTGCGATGAACTCGTCACCCTCCACGCGtt              >  2:26169/1‑149 (MQ=255)
                                                                                                                                     cAGAAAGCCCGCATGATTGCGGGCTTTCTGGTTCTTGTTCGTTCAGATTGCTTATTCTTCTGGCTTTCTGGCAAATAGCGCTTTCTGGTAGGCGTTATAGGAAAGCAGCCTGCTCCTCACTGCGATGAACTCGTCACCCTCCACGCGtt              >  2:156830/1‑149 (MQ=255)
                                                                                                                                      aGAAAGCCCGCAATCATGCGGGCTTTCTGGTTCTTGTTCGTTCAGATTGCTTATTCTTCTGGCTTTCTGGCAAATAGCGCTTTCTGGTAGGCGTtat                                                                 >  6:226760/1‑97 (MQ=38)
                                                                                                                                      aGAAAGCCCGCAATCATGCGGGCTTTCTGGTTCTTGTTCGTTCAGATTGCTTATTCTTCTGGCTTTCTGGCAAATAGCGCTTTCTGGTAGGCGTtat                                                                 <  5:226760/97‑1 (MQ=38)
                                                                                                                                                cATGATTGCGGGCTTTCTGGTTCTTGTTCGTTCAGATTGCTTATTCTTCTGGCTTTCTGGCAAATAGCGCTTTCTGGTAGGCGTTATAGGAAAGCAGCCTGCTCCTCACTGCGATGAACTCGTCACCCTCCACGCGTTGGGGGTGCttt   >  6:121948/1‑149 (MQ=255)
                                                                                                                                                cATGATTGCGGGCTTTCTGGTTCTTGTTCGTTCAGATTGCTTATTCTTCTGGCTTTCTGGCAAATAGCGCTTTCTGGTAGGCGTTATAGGAAAGCAGCCTGCTCCTCACTGCGATGAACTCGTCACCCTCCACGCGTTGGGGGTGCttt   <  1:156830/149‑1 (MQ=255)
                                                                                                                                                 aTGATTGCGGGCTTTCTGGTTCTTGTTCGTTCAGATTGCTTATTCTTCTGGCTTTCTGGCAAATAGCGCTTTCTGGTAGGCGTTATAGGAAAGCAGCCTGCTCCTCACTGCGATGAACTCGTCACCCTCCACGCGTTGGGGGTGCtttt  <  7:97705/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
CAGCGGTCGTATCATCAACGAAATCGACGGTATCTCGCGCGTTACCTACGACGTGTCGAGCAAGCCGCCTGCCACTATCGAGTGGGAGTGATCGGGTAGCCAGCCCAGGCTGGCACCCGCAGGACGGATACACCAGAAAGCCCGCATGATTGCGGGCTTTCTGGTTCTTGTTCGTTCAGATTGCTTATTCTTCTGGCTTTCTGGCAAATAGCGCTTTCTGGTAGGCGTTATAGGAAAGCAGCCTGCTCCTCACTGCGATGAACTCGTCACCCTCCACGCGTTGGGGGTGCTTTT  >  NC_002947/1178605‑1178898

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑