Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,178,754 T→A 8.2% intergenic (+59/+35) guaA → / ← PP_1033 glutamine‑hydrolyzing GMP synthase/sulfatase domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,178,7540TA 8.2% 139.8 / 3.4 49intergenic (+59/+35)guaA/PP_1033glutamine‑hydrolyzing GMP synthase/sulfatase domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base T (22/23);  new base A (2/2);  total (24/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.42e-01

GTCGTATCATCAACGAAATCGACGGTATCTCGCGCGTTACCTACGACGTGTCGAGCAAGCCGCCTGCCACTATCGAGTGGGAGTGATCGGGTAGCCAGCCCAGGCTGGCACCCGCAGGACGGATACACCAGAAAGCCCGCATGATTGCGGGCTTTCTGGTTCTTGTTCGTTCAGATTGCTTATTCTTCTGGCTTTCTGGCAAATAGCGCTTTCTGGTAGGCGTTATAGGAAAGCAGCCTGCTCCTCACTGCGATGAACTCGTCACCCTCCACGCGTTGGGGGTGCTTTT  >  NC_002947/1178610‑1178898
                                                                                                                                                |                                                                                                                                                
tgcGTATCTTCCACGACTTCGACGGGAGCTGGCGCGTGACCTACGACGTGTCGAGCAAGCCGCCTGCCACTATCGAGTGGGAGTGATCGGGTAGCCAGCCCAGGCTGGCACCCGCAGGACGGATACACCAGAAAGCCCGCATGATTGCg                                                                                                                                              <  4:186442/147‑1 (MQ=255)
gTCGTATCATGAACGTATTCGACGGTATCTCGCGCGTTACCTACGTCGTGTCGAGCAAGCCGCCTGCCACTATCGAGTGGGAGTGATCGGGTAGCCAGCCCAGGCTGGCACCCGCAGGACGGATACACCAGAAAGCCCGCATGATTGCg                                                                                                                                              <  4:189877/149‑1 (MQ=255)
   gTATCATCAACGAAATCGACGGTATCTCGCGCGTTACCTACGACGTGTCGAGCAAGCCGCCTGCCACTATCGAGTGGGAGTGATCGGGTAGCCAGCCCAGGCTGGCACCCGCAGGACGGATACACCAGAAAGCCCGCATGATTGCggg                                                                                                                                            <  4:234241/148‑1 (MQ=255)
   gTATCATCAACGAAATCGACGGTATCTCGCGCGTTACCTACGACGTGTCGAGCAAGCCGCCTGCCACTATCGAGTGGGAGTGATCGGGTAGCCAGCCCAGGCTGGCACCCGCAGGACGGATACACCAGAAAGCCCGCATGATTGCGGGc                                                                                                                                           <  6:227188/149‑1 (MQ=255)
        atcaACGAAATCGACGGTATCTCGCGCGTTACCTACGACGTGTCGAGCAAGCCGCCTGCCACTATCGAGTGGGAGTGATCGGGTAGCCAGCCCAGGCTGGCACCCGCAGGACGGATACACCAGAAAGCCCGCATGATTGCGGGCTTTCt                                                                                                                                      >  4:209401/1‑149 (MQ=255)
        atcaACGAAATCGACGGTATCTCGCGCGTTACCTACGACGTGTCGAGCAAGCCGCCTGCCACTATCGAGTGGGAGTGATCGGGTAGCCAGCCCAGGCTGGCACCCGCAGGACGGATACACCAGAAAGCCCGCATGATTGCGGGCTTTCt                                                                                                                                      <  3:209401/149‑1 (MQ=255)
         tcaACGAAATCGACGGTATCTCGCGCGTTACCTACGACGTGTCGAGCAAGCCGCCTGCCACTATCGAGTGGGAGTGATCGGGTAGCCAGCCCAGGCTGGCACCCGCAGGACGGATACACCAGAAAGCCCGCAATCATGCGGGCTTTCt                                                                                                                                      >  8:246907/1‑148 (MQ=255)
         tcaACGAAATCGACGGTATCTCGCGCGTTACCTACGACGTGTCGAGCAAGCCGCCTGCCACTATCGAGTGGGAGTGATCGGGTAGCCAGCCCAGGCTGGCACCCGCAGGACGGATACACCAGAAAGCCCGCAATCATGCGGGCTTTCt                                                                                                                                      <  7:246907/148‑1 (MQ=255)
           aaCGAAATCGACGGTATCTCGCGCGTTACCTACGACGTGTCGAGCAAGCCGCCTGCCACTATCGAGTGGGAGTGATCGGGTAGCCAGCCCAGGCTGGCACCCGCAGGACGGATACACCAGAAAGCCCGCATGATTGCGGGCTTTCTGGt                                                                                                                                   >  7:201953/1‑149 (MQ=255)
            aCGAAATCGACGGTATCTCGCGCGTTACCTACGACGTGTCGAGCAAGCCGCCTGCCACTATCGAGTGGGAGTGATCGGGTAGCCAGCCCAGGCTGGCACCCGCAGGACGGATACACCAGAAAGCCCGCATGATTGCGGGCTTTCTGGtt                                                                                                                                  >  8:97705/1‑149 (MQ=255)
            aCGAAATCGACGGTATCTCGCGCGTTACCTACGACGTGTCGAGCAAGCCGCCTGCCACTATCGAGTGGGAGTGATCGGGTAGCCAGCCCAGGCTGGCACCCGCAGGACGGATACACCAGAAAGCCCGCATGATTGCGGGCTTTCTGGtt                                                                                                                                  >  5:55634/1‑149 (MQ=255)
              gAAATCGACGGTATCTCGCGCGTTACCTACGACGTGTCGAGCAAGACGCCTGCCACTATCGAGTGGGAGTGATCGGGTAGCCAGCCCAGGCTGGCACCCGCAGGACGGATACACCAGAAAGCCCGCATCATTTCGGGCTTTCTGGATCt                                                                                                                                >  6:149877/1‑149 (MQ=255)
                 aTCGACGGTATCTCGCGCGTTACCTACGACGTGTCGAGCAAGCCGCCTGCCACTATCGAGTGGGAGTGATCGGGTAGCCAGCCCAGGCTGGCACCCGCAGGACGGATACACCAGAAAGCCCGCATGATTGCGGGCTTTCTGGTTCTTgt                                                                                                                             >  5:250763/1‑149 (MQ=255)
                 aTCGACGGTATCTCGCGCGTTACCTACGACGTGTCGAGCAAGCCGCCTGCCACTATCGAGTGGGAGTGATCGGGTAGCCAGCCCAGGCTGGCACCCGCAGGACGGATACACCAGAAAGCCCGCATGATTGCGGGCTTTCTGGTTCTTgt                                                                                                                             >  6:148057/1‑149 (MQ=255)
                    gACGGTATCTCGCGCGTTACCTACGACGTGTCGAGCAAGCCGCCTGCCACTATCGAGTGGGAGTGATCGGGTAGCCAGCCCAGGCTGGCACCCGCAGGACGGATACACCAGAAAGCCCGCATGATTGCGGGCTTTCTGGTTCTTgttc                                                                                                                           <  5:74962/148‑1 (MQ=255)
                    gACGGTATCTCGCGCGTTACCTACGACGTGTCGAGCAAGCCGCCTGCCACTATCGAGTGGGAGTGATCGGGTAGCCAGCCCAGGCTGGCACCCGCAGGACGGATACACCAGAAAGCCCGCATGATTGCGGGCTTTCTGGTTCTTgttc                                                                                                                           <  6:55634/148‑1 (MQ=255)
                     aCGGTATCTCGCGCGTTACCTACGACGTGTCGAGCAAGCCGCCTGCCACTATCGAGTGGGAGTGATCGGGTAGCCAGCCCAGGCTGGCACCCGCAGGACGGATACACCAGAAAGCCCGCATGATTGCGGGCTTTCTGGTTCTTgttcgt                                                                                                                         >  6:48641/1‑149 (MQ=255)
                           tctcGCGCGTTACCTACGACGTGTCGAGCAAGCCGCCTGCCACTATCGAGTGGGAGTGATCGGGTAGCCAGCCCAGGCTGGCACCCGCAGGACGGATACACCAGAAAGCCCGCATGATTGCGGGCTTTCTGGTTCTTGTTCGTTCAGa                                                                                                                    <  2:176489/148‑1 (MQ=255)
                              cgcgcgTTACCTACGACGTGTCGAGCAAGCCGCCTGCCACTATCGAGTGGGAGTGATCGGGTAGCCAGCCCAGGCTGGCACCCGCAGGACGGATACACCAGAAAGCCCGCATGATTGCGGGCTTTCTGGTTCTTGTTCGTTCAGAt                                                                                                                   >  1:108472/1‑146 (MQ=255)
                              cgcgcgTTACCTACGACGTGTCGAGCAAGCCGCCTGCCACTATCGAGTGGGAGTGATCGGGTAGCCAGCCCAGGCTGGCACCCGCAGGACGGATACACCAGAAAGCCCGCATGATTGCGGGCTTTCTGGTTCTTGTTCGTTCAGAt                                                                                                                   <  2:108472/146‑1 (MQ=255)
                               gcgcgTTACCTACGACGTGTCGAGCAAGCCGCCTGCCACTATCGAGTGGGAGTGATCGGGTAGCCAGCCCAGGCTGGCACCCGCAGGACGGATACACCAGAAAGCCCGCATGATTGCGGGCTTTCTGGTTCTTGTTCGTTCAGATTGc                                                                                                                <  5:48641/148‑1 (MQ=255)
                                  cgTTACCTACGACGTGTCGAGCAAGCCGCCTGCCACTATCGAGTGGGAGTGATCGGGTAGCCAGCCCAGGCTGGCACCCGCAGGACGGATACACCAGAAAGCCCGCATGATTGCGGGCTTTCTGGTTCTTGTTCGTTCAGATTGCTTAt                                                                                                            >  1:208139/1‑149 (MQ=255)
                                           cgacgTGTCGAGCAAGCCGCCTGCCACTATCGAGTGGGAGTGATCGGGTAGCCAGCCCAGGCTGGCACCCGCAGGACGGATACACCAGAAAGCCCGCATGATTGCGGGCTTTCTGGTTCTTGTTCGTTCAGATTGCTTATTCTTCTg                                                                                                     <  6:250763/147‑1 (MQ=255)
                                            gacgTGTCGAGCAAGCCGCCTGCCACTATCGAGTGGGAGTGATCGGATAGCCAGCCCAGGCTGGCACCCGCAGGACGGATACACCAGAAAGCCCGCATGATTGCGGGCTTTCTGGTTCTTGTTCGTTCAGATTGCTTATtcttct                                                                                                      >  6:211188/1‑145 (MQ=255)
                                            gacgTGTCGAGCAAGCCGCCTGCCACTATCGAGTGGGAGTGATCGGATAGCCAGCCCAGGCTGGCACCCGCAGGACGGATACACCAGAAAGCCCGCATGATTGCGGGCTTTCTGGTTCTTGTTCGTTCAGATTGCTTATtcttct                                                                                                      <  5:211188/145‑1 (MQ=255)
                                               gtgtCGAGCAAGCCGCCTGCCACTATCGAGTGGGAGTGATCGGGTAGCCAGCCCAGGCTGGCACCCGCAGGACGGATACACCAGAAAGCCCGCATGATTGCGGGCTTTCTGGTTCTTGTTCGTTCAGATTGCTTATTCTTCTGGCTTTc                                                                                               <  7:15646/149‑1 (MQ=255)
                                                tgtCGAGCAAGCCGCCTGCCACTATCGAGTGTGAGTGATCGGGTAGCGAGCCCAGGCTGGCACCCGCATGACGGATACACCAGAAAGCCCGCATGTTTGCGGGCTTTCTGGTTCTTGTTCGTTCAGATTGCTTATTCTTCTGGCTTTCt                                                                                              <  5:149877/149‑1 (MQ=255)
                                                   cGAGCAAGCCGCCTGCCACTATCGAGTGGGAGTGATCGGGTAGCCAGCCCAGGCTGGCACCCGCAGGACGGATACACCAGAAAGCCCGCATGATTGCGGGCTTTCTGGTTCTTGTTCGTTCAGATTGCTTATTCTTCTGGCTTTCTgg                                                                                            >  5:186705/1‑148 (MQ=255)
                                                                  ccACTATCGAGTGGGAGTGATCGGGTAGCCAGCCCAGGCTGGCACCCGCAGGACGGATACGCCAGAAAGCCCGCATGATTGCGGGCTTTCTGGTTCTTGTTCGTTCAGATTGCTTATTCTTCTGGCTTTCTGGCAAATAGCGCTTTc                                                                              <  4:251619/147‑1 (MQ=255)
                                                                        tCGAGTGGGAGTGATCGGGTAGCCAGCCCAGGCTGGCACCCGCAGGACGGATACACCAGAAAGCCCGCATGATTGCGGGCTTTCTGGTTCTTGTTCGTTCAGATTGCTTATTCTTCTGGCTTTCTGGCAAATAGCGCTTTCTGGTAGGc                                                                      <  5:148057/149‑1 (MQ=255)
                                                                           aGTGGGAGTGATCGGGTAGCCAGCCCAGGCTGGCACCCGCAGGACGGATACACCAGAAAGCCCGCATGATTGCGGGCTTTCTGGTTCTTGTTCGTTCAGATTGCTTATTCTTCTGGCTTTCTGGCAAATAGCGCTTTCTGGTAGGCGtt                                                                   >  8:157779/1‑149 (MQ=255)
                                                                            gttggAGTGATCGGGTAGCCAGCCCAGGCTGGCACCCGCAGGACGGATACACCAGAAAGCCCGCATGATTGCGGGCTTTCTGGTTCTTGTTCGTTCAGATTGCTTATTCTTCTGGCTTTCTGGCAAATAGCGCTTTCTGGTa                                                                         <  3:159965/139‑1 (MQ=255)
                                                                            gTGGGAGTGATCGGGTAGCCAGCCCAGGCTGGCACCCGCAGGACGGATACACCAGAAAGCCCGCATGATTGCGGGCTTTCTGGTTCTTGTTCGTTAAGATTGCTTATTCTTCTGGCTTTCTGGCAAATAGCGCTTTCTGGTa                                                                         >  4:159965/1‑142 (MQ=255)
                                                                             tGGGAGTGATCGGGTAGCCAGCCCAGGCTGGCACCCGCAGGACGGATACACCAGAAAGCCCGCATGATTGCGGGCTTTCTGGTTCTTGTTCGTTCAGATTGCTTATTCTTCTGGCTTTCTGGCAAATAGCGCTTTCTGGTAGGCGTtat                                                                 <  2:160786/149‑1 (MQ=255)
                                                                                        gggTAGCCAGCCCAGGCTGGCACCCGCAGGACGGATACACCAGAAAGCCCGCATGATTGCGGGCTTTCTGGTTCTTGTTCGTTCAGATTGCTTATTCTTCTGGCTTTCTGGCAAATAGCGCTTTCTGGTAGGCGTTATAGGAAagcagc                                                      <  2:208139/149‑1 (MQ=255)
                                                                                             gccagccCAGGCTGGCACCCGCAGGACGGATACACCAGAAAGCCCGCATGATTGCGGGCTTTCTGGTTCTTGTTCGTTCAGATTGCTTATTCTTCTGGCTTTCTGGCAAATAGCGCTTTCTGGTAGGCGTTATAGGAAAGCAGCCTGct                                                 >  1:163891/1‑149 (MQ=255)
                                                                                                                              acCAGAAAGCCCGCATGATTGCGGGCTTTCTGGTTCTTGTTCGTTCAGATTGCTTATTCTTCTGGCTTTCTGGCAAATAGCGCTTTCTGGTAGGCGTTATAGGAAAGCAGCCTGCTCCTCACTGCGATGAACTCGTCACCCTc                      >  4:204600/1‑143 (MQ=255)
                                                                                                                              acCAGAAAGCCCGCATGATTGCGGGCTTTCTGGTTCTTGTTCGTTCAGATTGCTTATTCTTCTGGCTTTCTGGCAAATAGCGCTTTCTGGTAGGCGTTATAGGAAAGCAGCCTGCTCCTCACTGCGATGAACTCGTCACCCTc                      <  3:204600/143‑1 (MQ=255)
                                                                                                                              acCAGAAAGCCCGCATGATTGCGGGCTTTCTGGTTCTTGTTCGTTCAGATTGCTTATTCTTCTGGCTTTCTGGCAAATAGCGCTTTCTGGTAGGCGTTATAGGAAAGCAGCCTGCTCCTCACTGCGATGAACTCGTCACCCTCCAcgcg                >  1:122697/1‑149 (MQ=255)
                                                                                                                              acCAGAAAGCCCGCATGATTGCGGGCTTTCTGGTTCTTGTTCGTTCAGATTGCTTATTCTTCTGGCTTTCTGGCAAATAGCGCTTTCTGGTAGGCGTTATAGGAAAGCAGCCTGCTCCTCACTGCGATGAACTCGTCACCCTCCAcgcg                >  7:35000/1‑149 (MQ=255)
                                                                                                                                cAGAAAGCCCGCATGATTGCGGGCTTTCTGGTTCTTGTTCGTTCAGATTGCTTATTCTTCTGGCTTTCTGGCAAATAGCGCTTTCTGGTAGGCGTTATAGGAAAGCAGCCTGCTCCTCACTg                                         <  6:85539/122‑1 (MQ=255)
                                                                                                                                cAGAAAGCCCGCATGATTGCGGGCTTTCTGGTTCTTGTTCGTTCAGATTGCTTATTCTTCTGGCTTTCTGGCAAATAGCGCTTTCTGGTAGGCGTTATAGGAAAGCAGCCTGCTCCTCACTg                                         >  5:85539/1‑122 (MQ=255)
                                                                                                                                cAGAAAGCCCGCATGATTGCGGGCTTTCTGGTTCTTGTTCGTTCAGATTGCTTATTCTTCTGGCTTTCTGGCAAATAGCGCTTTCTGGTAGGCGTTATAGGAAAGCAGCCTGCTCCTCACTGCGATGAACTCGTCACCCTCCACGCGtt              >  2:26169/1‑149 (MQ=255)
                                                                                                                                cAGAAAGCCCGCATGATTGCGGGCTTTCTGGTTCTTGTTCGTTCAGATTGCTTATTCTTCTGGCTTTCTGGCAAATAGCGCTTTCTGGTAGGCGTTATAGGAAAGCAGCCTGCTCCTCACTGCGATGAACTCGTCACCCTCCACGCGtt              >  2:156830/1‑149 (MQ=255)
                                                                                                                                 aGAAAGCCCGCAATCATGCGGGCTTTCTGGTTCTTGTTCGTTCAGATTGCTTATTCTTCTGGCTTTCTGGCAAATAGCGCTTTCTGGTAGGCGTtat                                                                 >  6:226760/1‑97 (MQ=38)
                                                                                                                                 aGAAAGCCCGCAATCATGCGGGCTTTCTGGTTCTTGTTCGTTCAGATTGCTTATTCTTCTGGCTTTCTGGCAAATAGCGCTTTCTGGTAGGCGTtat                                                                 <  5:226760/97‑1 (MQ=38)
                                                                                                                                           cATGATTGCGGGCTTTCTGGTTCTTGTTCGTTCAGATTGCTTATTCTTCTGGCTTTCTGGCAAATAGCGCTTTCTGGTAGGCGTTATAGGAAAGCAGCCTGCTCCTCACTGCGATGAACTCGTCACCCTCCACGCGTTGGGGGTGCttt   >  6:121948/1‑149 (MQ=255)
                                                                                                                                           cATGATTGCGGGCTTTCTGGTTCTTGTTCGTTCAGATTGCTTATTCTTCTGGCTTTCTGGCAAATAGCGCTTTCTGGTAGGCGTTATAGGAAAGCAGCCTGCTCCTCACTGCGATGAACTCGTCACCCTCCACGCGTTGGGGGTGCttt   <  1:156830/149‑1 (MQ=255)
                                                                                                                                            aTGATTGCGGGCTTTCTGGTTCTTGTTCGTTCAGATTGCTTATTCTTCTGGCTTTCTGGCAAATAGCGCTTTCTGGTAGGCGTTATAGGAAAGCAGCCTGCTCCTCACTGCGATGAACTCGTCACCCTCCACGCGTTGGGGGTGCtttt  <  7:97705/149‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                
GTCGTATCATCAACGAAATCGACGGTATCTCGCGCGTTACCTACGACGTGTCGAGCAAGCCGCCTGCCACTATCGAGTGGGAGTGATCGGGTAGCCAGCCCAGGCTGGCACCCGCAGGACGGATACACCAGAAAGCCCGCATGATTGCGGGCTTTCTGGTTCTTGTTCGTTCAGATTGCTTATTCTTCTGGCTTTCTGGCAAATAGCGCTTTCTGGTAGGCGTTATAGGAAAGCAGCCTGCTCCTCACTGCGATGAACTCGTCACCCTCCACGCGTTGGGGGTGCTTTT  >  NC_002947/1178610‑1178898

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑