Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,189,035 A→G 7.7% intergenic (+25/+17) PP_1038 → / ← tatC‑I hypothetical protein/Sec‑independent protein translocase protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,189,0350AG 7.7% 141.1 / 3.2 52intergenic (+25/+17)PP_1038/tatC‑Ihypothetical protein/Sec‑independent protein translocase protein
Reads supporting (aligned to +/- strand):  ref base A (26/22);  new base G (2/2);  total (28/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.58e-01

GAAGAGTGGAAGGTGGAGCTGGTGGTGGCTGACGAGCTGATTGTGCAGGTCGTCGCTGCGCTCAAGCAGAGCCACCCGTACGAGACGCCAGCCTATGAAGTCTGGCGGCTCGCCGAGTTCTAGAACGCATCACGGCTATTCGCGGGCACGCCCGCGAAAGGGCCCTACAATCCAGCCACAATCTCCTTGCCTGGCTCCGGCCGCTTCAACATTCCACAAGCCAGCAACCCCATCTCGAACAGCACCCACATCGGCACTGCCAGCATCGTCTGCGAAAACACATCCGGCGGTGTCAG  >  NC_002947/1188888‑1189183
                                                                                                                                                   |                                                                                                                                                    
gAAGAGTGGAAGGTGGAGCTGGTGGTGGCTGACGAGCTGATTGTGCAGGTCGTCGCTGCGCTCAAGCAGAGCCACCCGTACGAGACGCCAGCCTATGAAGTCTGGCGGCTCGCCGAGTTCTAGAACGCATCACGGCTATTCGCGGGCAc                                                                                                                                                     >  5:37037/1‑149 (MQ=255)
    agTGGAAGGTGGAGCTGGTGGTGGCTGACGAGCTGATTGTGCAGGTCGTCGCTGCGCTCAAGCAGAGCCACCCGTACGAGACGCCAGCCTATGAAGTCTGGCGGCTCGCCGAGTTCTAGAACGCATCACGGCTATTCGCGGGCACGccc                                                                                                                                                 <  6:109014/149‑1 (MQ=255)
        gAAGGTGGAGCTGGTGGTGGCTGACGAGCTGATTGTGCAGGTCGTCGCTGCGCTCAAGCAGAGCCACCCGTACGAGACGCCAGCCTATGAAGTCTGGCGGCTCGCCGAGTTCTAGAACGCATCACGGCTATTCGCGGGCACGCCcgcg                                                                                                                                              >  6:265032/1‑148 (MQ=255)
              ggAGCTGGTGGTGGCTGACGAGCTGATTGTGCAGGTCGTCGCTGCGCTCAAGCAGAGCCACCCGTACGAGACGCCAGCCTATGAAGTCTGGCGGCTCGCCGAGTTCTAGAACGCATCACGGCTATTCGCGGGCGTGCCCGCGaatagcc                                                                                                                                       <  7:250151/149‑6 (MQ=255)
               gAGCTGGTGGTGGCTGACGAGCTGATTGTGCAGGTCGTCGCTGCGCTCAAGCAGAGCCACCCGTACGAGACGCCAGCCTATGAAGTCTGGCGGCTCGCCGAGTTCTAGAACGCATCACGGCTATTCGCGGGCACGCCCGCGAAAGGGcc                                                                                                                                      >  7:94885/1‑149 (MQ=255)
               gAGCTGGTGGTGGCTGACGAGCTGATTGTGCAGGTCGTCGCTGCGCTCAAGCAGAGCCACCCGTACGAGACGCCAGCCTATGAAGTCTGGCGGCTCGCCGAGTTCTAGAACGCATCACGGCTATTCGCGGGCACGCCCGCGAAAGGGcc                                                                                                                                      <  7:85733/149‑1 (MQ=255)
               gAGCTGGTGGTGGCTGACGAGCTGATTGTGCAGGTCGTCGCTGCGCTCAAGCAGAGCCACCCGTACGAGACGCCAGCCTATGAAGGCTGGCGGCTCGCCGAGTTCTAGAACGCATCACGGCTATTCGCGGGCACGCCCGCGAAAGGGcc                                                                                                                                      <  5:208229/149‑1 (MQ=255)
                aGCTGGTGGTGGCTGACGAGCTGATTGTGCAGGTCGTCGCTGCGCTCAAGCAGAGCCACCCGTACGAGACGCCAGCCTATGAAGTCTGGCGGCTCGCCGAGTTCTAGAACGCATCACGGCTATTCGCGGGCACGCCCGCGAAAGGGccc                                                                                                                                     <  5:265032/149‑1 (MQ=255)
                        gtggCTGACGAGCTGATTGTGCAGGTCGTCGCTGCGCTCAAGCAGAGCCACCCGTACGAGACGCCAGCCTATGAAGTCTGGCGGCTCGCCGAGTTCTAGAACGCATCACGGCTATTCGCGGGCACGCCCGCGAAAGGGCCCTACAATc                                                                                                                              >  5:271503/1‑148 (MQ=255)
                           gCTGACGAGCTGATTGTGCAGGTCGTCGCTGCGCTCAAGCAGAGCCACCCGTACGAGACGCCAGCCTATGAAGTCTGGCGGCTCGCCGAGTTCTAGAACGCATCACGGCTATTCGCGGGCACGCCCGCGAAAGGGCCCTACAATCCAGc                                                                                                                          >  4:63972/1‑149 (MQ=255)
                                    cTGATTGTGCAGGTCGTCGCTGCGCTCAAGCAGAGCCACCCGTACGAGACGCCAGCCTATGAAGTCTGGCGGCTCGCCGAGTTCTAGAACGCATCACGGCTATTCGCGGGCGTGCCCGCGaatagccg                                                                                                                                      >  6:253601/1‑122 (MQ=255)
                                    cTGATTGTGCAGGTCGTCGCTGCGCTCAAGCAGAGCCACCCGTACGAGACGCCAGCCTATGAAGTCTGGCGGCTCGCCGAGTTCTAGAACGCATCACGGCTATTCGCGGGCGTGCCCGCGaatagccg                                                                                                                                      <  5:253601/128‑7 (MQ=255)
                                    cTGATTGTGCAGGTCGTCGCTGCGCTCAAGCAGAGCCACCCGTACGAGACGCCAGCCTATGAAGTCTGGCGGCTCGCCGAGTTCTAGAACGCATCACGGCTATTCGCGGGCACGCCCGCGAAAGGGCCCTACAATCCAGCCACAAtctc                                                                                                                 <  3:63972/149‑1 (MQ=255)
                                      gATTGTGCAGGTCGTCGCTGCGCTCAAGCAGAGCCACCCGTACGAGACGCCAGCCTATGAAGTCTGGCGGCTCGCCGAGTTCTAGAACGCATCACGGCTATTCGCGGGCACGCCCGCGAAAGGGCCCTACAATCCAGCCACAATCTCCt                                                                                                               >  7:185537/1‑149 (MQ=255)
                                      gATTGTGCAGGTCGTCGCTGCGCTCAAGCAGAGCCACCCGTACGAGACGCCAGCCTATGAAGTCTGGCGGCTCGCCGAGTTCTAGAACGCATCACGGCTATTCGCGGGCACGCCCGCGAAAGGGCCCTACAATCCAGCCACAATCTCCt                                                                                                               >  8:63165/1‑149 (MQ=255)
                                             cAGGTCGTCGCTGCGCTCAAGCAGAGACACCCGTACGAGACGCCAGCCTATGAAGTCTGGCGGCTCGCCGAGTTCTAGAACGCATCACGGCTATTCGCGGGCACGCCCGCGAAAGGGCCCTACAATCCAGCCACAATCTCCTTGCCTgg                                                                                                        >  2:62103/1‑149 (MQ=255)
                                                gtcgtcGCTGCGCTCAAGCAGAGCCACCCGTACGAGACGCCAGCCTATGAAGTCTGGCGGCTCGCCGAGTTCTAGAACGCATCACGGCTATTCGCGGGCACGCCCGCGAAAGGGCCCTACAATCCAGCCACAATCTCCTTGCCTGGCTc                                                                                                     >  7:112498/1‑149 (MQ=255)
                                                   gtcgCTGCGCTCAAGCAGAGCCACCCGTACGAGACGCCAGCCTATGAAGTCTGGCGGCTCGCCGAGTTCTAGAACGCATCACGGCTATTCGCGGGCACGCCCGCGAAAGGGCCCTACAATCCAGCCACAATCTCCTTGCCTGGCTCCg                                                                                                   <  8:94885/148‑1 (MQ=255)
                                                    tcgCTGCGCTCAAGCAGAGCCACCCGTACGAGACGCCAGCCTATGAAGTCTGGCGGCTCGCCGAGTTCTAGAACGCATCACGGCTATTCGCGGGCACGCCCGCGAAAGGGCCCTACAATCCAGCCACAATCTCCTTGCCTGGCTCCGGc                                                                                                 <  7:63165/149‑1 (MQ=255)
                                                       cTGCGCTCAAGCAGAGCCACCCGTACGAGACGCCAGCCTATGAAGTCTGGCGGCTCGCCGAGTTCTAGAACGCATCACGGCTATTCGCGGGCACGCCCGCGAAAGGGCCCTACAATCCAGCCACAATCTCCTTGCCTGGCTCCGGCCGc                                                                                              <  4:218994/149‑1 (MQ=255)
                                                            cTCAAGCAGAGCCACCCGTACGAGACGCCAGCCTATGAAGTCTGGCGGCTCGCCGAGTTCTAGAACGCATCACGGCTATTCGCGGGCACGCCTGCGAAAGGGCCCTACAATCCAGCCACAATCTCCTTGCCTGGCTCCGGCCGCTTCaa                                                                                         >  7:183830/1‑149 (MQ=255)
                                                                 gCAGAGCCACCCGTACGAGACGCCAGCCTATGAAGTCTGGCGGCTCGCCGAGTTCTAGAACGCATCACGGCTATTCGCGGGCACGCCCGCGAAAGGGCCCTACAATCCAGCCACAATCTCCTTGCCTGGCTCCGGCCGCTTCAACATTc                                                                                    <  8:185537/149‑1 (MQ=255)
                                                                          cccGTACGAGACGCCAGCCTATGAAGTCTGGCGGCTCGCCGAGTTCTAGAACGCATCACGGCTATTCGCGGGCACGCCCGCGAAAGGGCCCTACAATCCAGCCACAATCTCCTTGCCTGGCTCCGGCCGCTTCAACATTCCACAAGc                                                                             >  5:261042/1‑147 (MQ=255)
                                                                          cccGTACGAGACGCCAGCCTATGAAGTCTGGCGGCTCGCCGAGTTCTAGAACGCATCACGGCTATTCGCGGGCACGCCCGCGAAAGGGCCCTACAATCCAGCCACAATCTCCTTGCCTGGCTCCGGCCGCTTCAACATTCCACAAGc                                                                             <  6:261042/147‑1 (MQ=255)
                                                                            cGTACGAGACGCCAGCCTATGAAGTCTGGCGGCTCGCCGAGTTCTAGAACGCATCACGGCTATTCGCGGGCACGCCCGCGAAAGGGCCCTACAATCCAGCCACAATCTCCTTGCCTGGCTCCGGCCGCTTCAACATTCCACAAGCCAGc                                                                         <  6:271503/149‑1 (MQ=255)
                                                                             gTACGAGACGCCAGCCTATGAAGTCTGGCGGCTCGCCGAGTTCTAGAACGCATCACGGCTATTCGCGGGCACGCCCGCGAAAGGGCCCTACAATCCAGCCACAATCTCCTTGCCTGGCTCCGGCCGCTTCAACATTCCACAAGCCaac                                                                         >  1:187667/1‑146 (MQ=255)
                                                                              tACGAGACGCCAGCCTATGAAGTCTGGCGGCTCGCCGAGTTCTAGAACGCATCACGGCTATTCGCGGGCACGCCCGCGAAAGGGCCCTACAATCCAGCCACAATCTCCTTGCCTGGCTCCGGCCGCTTCAACATTCCACAAGCCAGCaa                                                                       >  6:104764/1‑149 (MQ=255)
                                                                                 gagaCGCCAGCCTATGAAGTCTGGCGGCTCGCCGAGTTCTAGAACGCATCACGGCTATTCTCGGGCACGACCGCGAAAGGGCCCTACAAACCAGCCACAATCTCCTTGAAAGGCTCAGGCCGCTTCAACAATACACAACCCACCAAcc                                                                     >  5:168356/1‑148 (MQ=255)
                                                                                    aCGCCAGCCTATGAAGTCTGGCGGCTCGCCGAGTTCTAGAACGCATCACGGCTATTCGCGGGCACGCCCGCGAAAGGGCCCTACAATCCAGCCACAATCTCCTTGCCTGGCTCCGGCCGCTTCAACATTCCACAAGc                                                                             <  8:87538/137‑1 (MQ=255)
                                                                                    aCGCCAGCCTATGAAGTCTGGCGGCTCGCCGAGTTCTAGAACGCATCACGGCTATTCGCGGGCACGCCCGCGAAAGGGCCCTACAATCCAGCCACAATCTCCTTGCCTGGCTCCGGCCGCTTCAACATTCCACAAGc                                                                             >  7:87538/1‑137 (MQ=255)
                                                                                    aCGCCAGCCTATGAAGTCTGGCGGCTCGCCGAGTTCTAGAACGCATCACGGCTATTCGCGGGCACGCCCGCGAAAGGGCCCTACAATCCAGCCACAATCTCCTTGCCTGGCTCCGGCCGCTTCAACATTCCACAAGCCAGCAACCCCAt                                                                 <  6:821/149‑1 (MQ=255)
                                                                                      gCCAGCCTATGAAGTCTGGCGGCTCGCCGAGTTCTAGAACGCATCACGGCTATTCGCGGGCACGCCCGCGAAAGGGCCCTACAATCCAGCCACAATCTCCTTGCCTGGCTCCGGCCGCTTCAACATTCCACaa                                                                               <  6:77500/133‑1 (MQ=255)
                                                                                      gCCAGCCTATGAAGTCTGGCGGCTCGCCGAGTTCTAGAACGCATCACGGCTATTCGCGGGCACGCCCGCGAAAGGGCCCTACAATCCAGCCACAATCTCCTTGCCTGGCTCCGGCCGCTTCAACATTCCACaa                                                                               >  5:77500/1‑133 (MQ=255)
                                                                                       ccAGCCTATGAAGTCTGGCGGCTCGCCGAGTTCTAGAACGCATCACGGCTATTCGCGGGCACGCCCGCGAAAGGGCCCTACAATCCAGCCACAATCTCCTTGCCTGGCTCCGGCCGCTTCAACATTCCACAAGCCAGCAACCCCAtctc                                                              <  1:62103/149‑1 (MQ=255)
                                                                                         aGCCTATGAAGTCTGGCGGCTCGCCGAGTTCTAGAACGCATCACGGCTATTCGCGGGCACGCCCGCGAAAGGGCCCTACAATCCAGCCACAATCTCCTTGCCTGGCTCCGGc                                                                                                 >  5:32939/1‑112 (MQ=255)
                                                                                         aGCCTATGAAGTCTGGCGGCTCGCCGAGTTCTAGAACGCATCACGGCTATTCGCGGGCACGCCCGCGAAAGGGCCCTACAATCCAGCCACAATCTCCTTGCCTGGCTCCGGc                                                                                                 <  6:32939/112‑1 (MQ=255)
                                                                                              aTGAAGTCTGGCGGCTCGCCGAGTTCTAGAACGCATCACGGCTATTCGCGGGCACGCCCGCGAAAGGGCCCTACAATCCAGCCACAATCTCCTTGCCTGGCTCCGGCCGCTTCAACATTCCACAAGCCAGCAACCCCATCTCGAACAGc                                                       >  7:78460/1‑149 (MQ=255)
                                                                                                         cggcTCGCCGAGTTCTAGAACGCATCACGGCTATTCGCGGGCACGCCCGCGAAAGGGCCCTACAATCCAGCCACAATCTCCTTGTCTGGCTCCGGCCGCTTCAACATTCCACAAGCCAGCAACCCCATCTCGAACAGCACCCACATCgg                                            <  6:37037/149‑1 (MQ=255)
                                                                                                           gcTCGCCGAGTTCTAGAACGCATCACGGCTATTCGCGGGCACGCCCGCGAAAGGGCCCTACAATCCAGCCACAATCTCCTTGCCTGGCTCCGGCCGCTTCAACATTCCACAAGCCAGCAACCCCATCTCGAACAGCACCCACATCGGCa                                          >  5:32957/1‑149 (MQ=255)
                                                                                                             tCGCCGAGTTCTAGAACGCATCACGGCTATTCGCGGGCACGCCCGCGAAAGGGCCCTACAATCCAGCCACAATCTCCTTGCCTGGCTCCGGCCGCTTCAACATTCCACAAGCCAGCAACCCCATCTCGAACAGCACCCACATCGGCACt                                        >  2:208865/1‑149 (MQ=255)
                                                                                                              cGCCGAGTTCTAGAACGCATCACGGCTATTCGCGGGCACGCCCGCGAAAGGGCCCTACAATCCAGCCACAATCTCCTTGCCTGGCTCCGGCCGCTTCAACATTCCACAAGCCAGCAACCCCATCTCGAACAGCACCCACATCGGCACTg                                       >  7:230082/1‑149 (MQ=255)
                                                                                                               gCCGAGTTCTAGAACGCATCACGGCTATTCGCGGGCACGCCTGCGAAAGGGCCCTACAATCCAGCCACAATCTCCTTGCCTGGCTCCGGCCGCTTCAACATTCCACAAGCCAGCAACCCCATCTCGAACAGCACCCACATCGGCACTGc                                      <  8:183830/149‑1 (MQ=255)
                                                                                                               gCCGAGTTCTAGAACGCATCACGGCTATTCGCGGGCACGCCCGCGAAAGGGCCCTACAATCCAGCCACAATCTCCTTGCCTGGCTCCGGCCGCTTCAACATTCCACAAGCCAGCAACCCCATCTCGAACAGCACCCACATCGGCACTg                                       <  6:32957/148‑1 (MQ=255)
                                                                                                                ccGAGTTCTAGAACGCATCACGGCTATTCGCGTGCACTCCCGCGAAAGGGCCCTACAATCCAGCCACAATCTCCTTGCCTGGCTCCGTCCGCTTCAACATTCCACAAGCCATCAACCCCATCTCGAACAGCACCCACATCGGCACTGcc                                     <  6:168356/149‑1 (MQ=255)
                                                                                                                ccGAGTTCTAGAACGCATCACGGCTATTCGCGGGCACGCCCGCGAAAGGGCCCTACAATCCAGCCACAATCTCCTTGCCTGGCTCCGGCCGCTTCAACATTCCACAAGCCAGCAACCCCATCTCGAACAGCACCCACATCGGCACTGcc                                     >  6:183018/1‑149 (MQ=255)
                                                                                                                        tAGAACGCATCACGGCTATTCGCGGGCACGCCCGCGAAAGGGCCCTACAATCCAGCCACAATCTCCTTGCCTGGCTCCGGCCGCTTCAACATTCCACAAGCCAGCAACCCCATCTCGAACAGCACCCACATCGGCACTGCCAGCATCg                              >  2:270444/1‑148 (MQ=255)
                                                                                                                        tAGAACGCATCACGGCTATTCGCGGGAACGCCCGCGAAAGGGCCCTACAATCCAGCCACAATCTCCTTGCCTGGCTCCGGCCGCTTCAACATTCCACAAGCCAGCAACCCCGTCTCGCACAGCCCCCACATCGGCACTGCCAGCATCGt                             >  6:178705/1‑149 (MQ=255)
                                                                                                                                   aCGGCTATTCGCGGGCACGCCCGCGAAAGGGCCCTACAATCCAGCCACAATCTCCTTGCCTGGCTCCGGCCGCTTCAACATTCCACAAGCCAGCAACCCCATCTCGAACAGCACCCACATCGGCACTGCCAGCATCGTCTGCGAAAaca                  >  7:66319/1‑149 (MQ=255)
                                                                                                                                   aCGGCTATTCGCGGGCACGCCCGCGAAAGGGCCCTACAATCCAGCCACAATCTCCTTGCCTGGCTCCGGCCGCTTCAACATTCCACAAGCCAGCAACCCCATCTCGAACAGCACCCACATCGGCACTGCCAGCATCGTCTGCGAAAac                   <  5:104764/148‑1 (MQ=255)
                                                                                                                                     ggCTATTCGCGGGCACGCCCGCGAAAGGGCCCTACAATCCAGCCACAATCTCCTTGCCTGGCTCCGGCCGCTTCAACATTCCACAAGCCAGCAACCCCATCTCGAACAGCACCCACATCGGCACTGCCAGCATCGTCTGCGAAAAcaca                >  7:178938/1‑149 (MQ=255)
                                                                                                                                         tttCGCGGGCGTGCCCGCGAAAGGGCCCTACAATCCAGCCACAATCTCCTTGCCTGGCTCCGGCCGCTTCAACATTCCACAAGCCAGCAACCCCATCTCGAACAGCACCCACATCGGCACTGCCAGCATCGTCTGCGAAAACACATCcg            >  3:22622/2‑149 (MQ=255)
                                                                                                                                                  cACGCCCGCGAAAGGGCCCTACAATCCAGCCACAATCTCCTTGCCTGGCTCCGGCCGCTTCAACATTCCACAAGCCAGCAACCCCATCTCGAACAGCACCCACATCGGCACTGCCAGCATCGTCTGCGAAAACACATCCGGCGGTGTCa   <  8:66319/149‑1 (MQ=255)
                                                                                                                                                   aCGCCCGCGAAAGGGCCCTACAATCCAGCCACAATCTCCTTGCCTGGCTCCGGCCGCTTCAACATTCCACAAGCCAGCAACCCCATCTCGAACAGCACCCACATCGGCACTGCCAGCATCGTCTGCGAAAACACATCCGGCGGTGTCAg  <  1:270444/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
GAAGAGTGGAAGGTGGAGCTGGTGGTGGCTGACGAGCTGATTGTGCAGGTCGTCGCTGCGCTCAAGCAGAGCCACCCGTACGAGACGCCAGCCTATGAAGTCTGGCGGCTCGCCGAGTTCTAGAACGCATCACGGCTATTCGCGGGCACGCCCGCGAAAGGGCCCTACAATCCAGCCACAATCTCCTTGCCTGGCTCCGGCCGCTTCAACATTCCACAAGCCAGCAACCCCATCTCGAACAGCACCCACATCGGCACTGCCAGCATCGTCTGCGAAAACACATCCGGCGGTGTCAG  >  NC_002947/1188888‑1189183

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑