Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,215,211	A→C	6.1%	V583G (GTG→GGG)	PP_1061 ←	ATP‑dependent DNA helicase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,215,211	0	A	C	6.1%	193.7 / 4.7	66	V583G (GTG→GGG)	PP_1061	ATP‑dependent DNA helicase
Reads supporting (aligned to +/- strand):  ref base A (29/33);  new base C (2/2);  total (31/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.88e-01

ACGGGTGCGGGCCAGGTAGTCGAGCAAC‑TGGCCCGCACAAGCTTCGTCCAGTTCGAAGGTCGCCTGCAACCAGGCCAGTACCTCCACCGTGTCGCCGCCTGCCAGCGCCAGTTGCTGGTCGATTCGCGCCTGCAGCCGGGCCACGGCGGCAGACAGCTCGTCACTGCGCCCCGGCGCTTCACCCAGCCAGAACGGAATGGTCGGTGGCAGGCCATGAGCATCCTCTACCCGCACCCGGCCCGGCTCGACGCGCAGAATGCGATAGGAGGCGTTGCCCAGCTGGAAAAT  >  NC_002947/1215069‑1215356                                                                                                                                                |                                                                                                                                                  aCGGGTGCGGGCCAGGTAGTCGAGCAAC‑TGGCCCGCACAAGCTTCGTCCAGCTCGAAGGTCGCCTGCAACCAGGCCAGTACCTCCACCGTGTCGCCGCCTGCCAGCGCCAGTTGCTGGTCGATTCGCGCCTGCAGCCGGGCCAcggcgg                                                                                                                                             <  5:247643/149‑1 (MQ=255)    ggTGCGGGCCAGGTAGTCGAGCAACTTGGCCCGCACAAGCTTCGTCCAGTTCGAAGGTCGCCTGCAACCAGGCCAGTACCTCCACCGTGTCGCCGCCTGCCAGCGCCAGTTGCTGGTCGATTCGCGCCTGCAGCCGGGCCAcggcgg                                                                                                                                             >  1:140179/1‑147 (MQ=255)      tGCGGGCCAGGTAGTCGAGCAAC‑TGGCCCGCACAAGCTTCGTCCAGTTCGAAGGTCGCCTGCAACCAGGCCAGTACCTCCACCGTGTCGCCGCCTGCCAGCGCCAGTTGCTGGTCGATTCGCGCCTGCCGCCGTGGCCCGGCTGCAggc                                                                                                                                        <  5:106419/149‑3 (MQ=255)       gCGGGCCAGTTGCTCGACTACC‑TGGCCCGCACAAGCTTCGTCCAGTTCGAAGGTCGCCTGCAACCAGGCCAGTACCTCCACCGTGTCGCCGCCTGCCAGCGCCAGTTGCTGGTCGATTCGCGCCTGCAGCCGGGCCACGGCGGCAGa                                                                                                                                         >  3:99218/1‑147 (MQ=255)        cGGGCCAGTTGCTCGACTACC‑TGGCCCGCACAAGCTTCGTCCAGTTCGAAGGTCGCCTGCAACCAGGCCAGTACCTCCACCGTGTCGCCGCCTGCCAGCGCCAGTTGCTGGTCGATTCGCGCCTGCAGCCGGGCCACGGCGGCAGACAg                                                                                                                                      >  7:9894/1‑149 (MQ=255)                      gAGCAAC‑TGGCCCGCACAAGCTTCGTCCAGTTCGAAGGTCGCCTGCAACCAGGCCAGTACCTCCACCGTGTCGCCGCCTGCCAGCGCCAGTTGCTGGTCGATTCGCGCCTGCAGCCGGGCCACGGCGGCAGACAGCTCGTCACTGCGcc                                                                                                                        >  3:168262/1‑149 (MQ=255)                          aaC‑TGGCCCGCACAAGCTTCGTCCAGTTCGAAGGTCGCCTGCAACCAGGCCAGTACCTCCACCGTGTCGCCGCCTGCCAGCGCCAGTTGCTGGTCGATTCGCGCCTGCAGCCGGGCCACGGCGGCAGACAGCTCGTCACt                                                                                                                             >  8:49543/1‑140 (MQ=255)                          aaC‑TGGCCCGCACAAGCTTCGTCCAGTTCGAAGGTCGCCTGCAACCAGGCCAGTACCTCCACCGTGTCGCCGCCTGCCAGCGCCAGTTGCTGGTCGATTCGCGCCTGCAGCCGGGCCACGGCGGCAGACAGCTCGTCACt                                                                                                                             <  7:49543/140‑1 (MQ=255)                           aC‑TGGCCCGCACAAGCTTCGTCCAGTTCGAAGGTCGCCTGCAACCAGGCCAGTACCTCCACCGTGTCGCCGCCTGCCAGCGCCAGTTGCTGGTCGATTCGCGCCTGCAGCCGGGCCACggcggc                                                                                                                                            <  3:243088/124‑1 (MQ=255)                           aC‑TGGCCCGCACAAGCTTCGTCCAGTTCGAAGGTCGCCTGCAACCAGGCCAGTACCTCCACCGTGTCGCCGCCTGCCAGCGCCAGTTGCTGGTCGATTCGCGCCTGCAGCCGGGCCACggcggc                                                                                                                                            >  4:243088/1‑124 (MQ=255)                                gCCCGCACAAGCTTCGTCCAGTTCGAAGGTCGCCTGCAACCAGGCCAGTACCTCCACCGTGTCGCCGCCTGCCAGCGCCAGTTGCTGGTCGATTCGCGCCTGCAGCCGGGCCACGGCGGCAGACAGCTCGTCACTGCGCCCCGgcgc                                                                                                                 <  8:9894/147‑1 (MQ=255)                                  ccGCACAAGCTTCGTCCAGTTCGAAGGTCGCCTGCAACCAGGCCAGTACCTCCACCGTGTCGCCGCCTGCCAGCGCCAGTTGCTGGTCGATTCGCGCCTGCAGCCGGGCCACGGCGGCAGACAGCTCGTCACTGCGCCCCGGCGCTTCa                                                                                                             >  4:52127/1‑149 (MQ=255)                                   cGCACAAGCTTCGTCCAGTTCGAAGGTCGCCTGCAACCAGGCCAGTACCTCCACCGTGTCGCCGCCTGCCAGCGCCAGTTGCTGGTCGATTCGCGCCTGCAGCCGGGCCACGGCGGCAGACAGCTCGTCACTGCGCCCCGGCGCTTCAc                                                                                                            <  3:205993/149‑1 (MQ=255)                                        aaGCTTCGTCCAGTTCGAAGGTCGCCTGCAACCAGGCCAGTACCTCCACCGTGTCGCCGCCTGCCAGCGCCAGTTGCTGGTCGATTCGCGCCTGCAGCCGGGCCACGGCGGCAGACAGCTCGTCACTGCGCCCCGGCGCTTCACccagc                                                                                                       <  8:35697/149‑1 (MQ=255)                                        aaGCTTCGTCCAGTTCGAAGGTCGCCTGCAACCAGGCCAGTACCTCCACCGTGTCGCCGCCTGCCAGCGCCAGTTGCTGGTCGATTCGCGCCTGCAGCCGGGCCACGGCGGCAGACAGCTCGTCACTGCGCCCCGGCGCTTCACccagc                                                                                                       >  8:150120/1‑149 (MQ=255)                                           cTTCGTCCAGTTCGAAGGTCGCCTGCAACCAGGCCAGTACCTCCACCGTGTCGCCGCCTGCCAGCGCCAGTTGCTGGTCGATTCGCGCCTGCAGCCGGGCCACGGCGGCAGACAGCTCGTCACTGCGCCCCGGCGCTTCACccagc                                                                                                       >  4:33183/1‑146 (MQ=255)                                               gTCCAGTTCGAAGGTCGCCTGCAACCAGGCCAGTACCTCCACCGTGTCGCCGCCTGCCAGCGCCAGTTGCTGGTCGATTCGCGCCTGCAGCCGGGCCACGGCGGCAGACAGCTCGTCACTGCGCCCCGGCGCTTCACCCAGCCAGAACg                                                                                                <  8:137167/149‑1 (MQ=255)                                                tCCAGTTCGAAGGTCGCCTGCAACCAGGCCAGTACCTCCACCGTGTCGCCGCCTGCCAGCGCCAGTTGCTGGTCGATTCGCGCCTGCAGCCGGGCCACGGCGGCAGACAGCTCGTCACTGCGCCCCGGCGCTTCACCCAGCCAGAACgg                                                                                               <  4:184401/149‑1 (MQ=255)                                                 ccAGTTCGAAGGTCGCCTGCAACCAGGCCAGTACCTCCACCGTGTCGCCGCCTGCCAGCGCCAGTTGCTGGTCGATTCGCGCCTGCAGCCGGGCCACGGCGGCAGACAGCTCGTCACTGCGCCCCGGCGCTTCACCCAGCCAGAACgg                                                                                               >  7:105696/1‑148 (MQ=255)                                                    gctCGAACGTCGCCTGCAACCAGGCCAGTACCTCCACCGCTTCTCCGCCTGCCAGCTCCAGTTGCTGGTAGATTCGCGCCTGCAGCCGGGCCACGGCGGCAGACAGCTCGTCACTTCGCCCCGGCGCTTCACCCAGCCATAACGGAAtt                                                                                           <  8:117599/147‑2 (MQ=255)                                                     ttCGAAGGTCGCCTGCAACCAGGCCAGTACCTCCACCGTGTCGCCGCCTGCCAGCGCCAGTTGCTGGTCGATTCGCGCCTGCAGCCGGGCCACGGCGGCAGACAGCTCGTCACTGCGCCCCGGCGCTTCACCCAGCCAGAACGGAATgg                                                                                          >  2:48018/1‑149 (MQ=255)                                                       cGAAGGTCGCCTGCAACCAGGCCAGTACCTCCACCGTGTCGCCGCCTGCCAGCGCCAGTTGCTGGTCGATTCGCGCCTGCAGCCGGGCCACGGCGGCAGACAGCTCGTCACTGCGCCCCGGCGCTTCACCCAGCCAGAACGGAATGGt                                                                                         <  5:115447/148‑1 (MQ=255)                                                        gAAGGTCGCCTGCAACCAGGCCAGTACCTCCACCGTGTCGCCGCCTGCCAGCGCCAGTTGCTGGTCGATTCGCGCCTGCAGCCGGGCCACGGCGGCAGACAGCTCGTCACTGCGCCCCGGCGCTTCACCCAGCCAGAACGGAATGGTCg                                                                                       <  7:150120/149‑1 (MQ=255)                                                         aaGGTCGCCTGCAACCAGGCCAGTACCTCCACCGTGTCGCCGCCTGCCAGCGCCAGTTGCTGGTCGATTCGCGCCTGCAGCCGGGCCACGGCGGCAGACAGCTCGTCACTGCGCCCCGGCGCTTCACCCAGCCAGAACGGAATGGTCgg                                                                                      <  1:33589/149‑1 (MQ=255)                                                             tCGCCTGCAACCAGGCCAGTACCTCCACCGTGTCGCCGCCTGCCAGCGCCAGTTGCTGGTCGATTCGCGCCTGCAGCCGGGCCACGGCGGCAGACAGCTCGTCACTGCGCCCCGGCGCTTCACCCAGCCAGAACGGAATGGTCGGTgg                                                                                   >  2:237705/1‑148 (MQ=255)                                                               gCCTGCAACCAGGCCAGTACCTCCACCGTGTCGCCGCCTGCCAGCGCCAGTTGCTGGTCGATTCGCGCCTGCAGCCGGGCCACGGCGGCAGACAGCTCGTCACTGCGCCCCGGCGCTTCACCCAGCCAGAACGGAATGGTCGGTGGCAg                                                                                <  8:105696/149‑1 (MQ=255)                                                                 cTGCAACCAGGCCAGTACCTCCACCGTGTCGCCGCCTGCCAGCGCCAGTTGCTGGTCGATTCGCGCCTGCCGCCGTGGCCcggc                                                                                                                                               >  7:78564/1‑84 (MQ=255)                                                                 cTGCAACCAGGCCAGTACCTCCACCGTGTCGCCGCCTGCCAGCGCCAGTTGCTGGTCGATTCGCGCCTGCCGCCGTGGCCcggc                                                                                                                                               <  8:78564/84‑1 (MQ=255)                                                                 cTGCAACCAGGCCAGTACCTCCACCGTGTCGCCGCCTGCCAGCGCCAGTTGCTGGTCGATTCGCGCCTGCAGCCGGGCCACGGCGGCAGACAGCTCGTCACTGCGCCCCGGCGCTTCACCCAGCCAGAACGGAATGGTCGGTGGCAGGc                                                                              <  3:52127/149‑1 (MQ=255)                                                                 cTGCAACCAGGCCAGTACCTCCACCGTGTCGCCGCCTGCCAGCGCCAGTTGCTGGTCGATTCGCGCCTGCAGCCGGGCCACGGCGGCAGACAGCTCGTCACTGCGCCCCGGCGCTTCACCCAGCCAGAACGGAATGGTCGGTGGCAGGc                                                                              >  4:53692/1‑149 (MQ=255)                                                                    cAACCAGGCCAGTACCTCCACCGTGTCGCCGCCTGCCAGCGCCAGTTGCTGGTCGATTCGCGCCTGCAGCCGGGCCACGGCGGCAGACAGCTCGTCACTGCGCCCCGGCGCTTCACCCAGCCAGAACGGAATGGTCGGTGGCAGGcc                                                                             <  4:99218/147‑1 (MQ=255)                                                                        cAGGCCAGTACCTCCACCGTGTCGCCGCCTGCCAGCGCCAGTTGCTGGTCGATTCGCGCCTGCAGCCGGGCCACGGCGGCAGACAGCTCGTCACTGCGCCCCGGCGCTTCACCCAGCCAGAACGGAATGGTCGGTGGCAGGCCATGAg                                                                        >  7:47608/1‑148 (MQ=255)                                                                        cAGGCCAGTACCTCCACCGTGTCGCCGCCTGCCAGCGCCAGTTGCTGGTCGATTCGCGCCTGCAGCCGGGCCACGGCGGCAGACAGCTCGTCACTGCGCCCCGGCGCTTCACCCAGCCAGAACGGAATGGTCGGTGGCAGGCCATGAGc                                                                       <  1:237705/149‑1 (MQ=255)                                                                         aGGCCAGTACCTCCACCGTGTCGCCGCCTGCCAGCGCCAGTTGCTGGTCGATTCGCGCCTGCAGCCGGGCCACGGCGGCAGACAGCTCGTCACTGCGCCCCGGCGCTTCACCCAGCCAGAACGGAATGGTCGTTGGCAGGCCATGAGCa                                                                      <  8:139729/149‑1 (MQ=255)                                                                         aGGCCAGTACCTCCACCGTGTCGCCGCCTGCCAGCGCCAGTTGCTGGTCGATTCGCGCCTGCAGCCGGGCCACGGCGGCAGACAGCTCGTCACTGCGCCCCGGCGCTTCACCCAGCCAGAACGGAATGGTCGGTGGCAGGCCATGAGCa                                                                      >  8:205992/1‑149 (MQ=255)                                                                          ggCCAGTACCTCCACCGTGTCGCCGCCTGCCAGCGCCAGTTGCTGGTCGATTCGCGCCTGCAGCCGGGCCACGGCGGCAGACAGCTCGTCACTGCGCCCCGGCGCTTCACCCAGCCAGAACGGAATGGTCGGTGGCAGGCCATGAGCAt                                                                     >  6:251988/1‑149 (MQ=255)                                                                             cAGTACCTCCACCGTGTCGCCGCCTGCCAGCGCCAGTTGCTGGTCGATTCGCGCCTGCAGCCGGGCCACGGCGGCAGACAGCTCGTCACTGCGCCCCGGCGCTTCACCCAGCCAGAACGGAATGGTCGGTGGCAGGCCATGAGCATCct                                                                  <  1:109147/149‑1 (MQ=255)                                                                                    tCCACCGTGTCGCCGCCTGCCAGCGCCAGTTGCTGGTCGATTCGCGCCTGCAGCCGGGCCACGGCGGCAGACAGCTCGTCACTGCGCCCCGGCGCTTCACCCAGCCAGAACGGAATGGTCGGTGGCAGGCCATGAGc                                                                       <  3:149901/137‑1 (MQ=255)                                                                                    tCCACCGTGTCGCCGCCTGCCAGCGCCAGTTGCTGGTCGATTCGCGCCTGCAGCCGGGCCACGGCGGCAGACAGCTCGTCACTGCGCCCCGGCGCTTCACCCAGCCAGAACGGAATGGTCGGTGGCAGGCCATGAGc                                                                       >  4:149901/1‑137 (MQ=255)                                                                                     ccACCGTGTCGCCGCCTGCCAGCGCCAGTTGCTGGTCGATTCGCGCCTGCAGCCGGGCCACGGCGGCAGACAGCTCGTCACTGCGCCCCGGCGCTTCACCCAGCCAGAACGGAATGGTCGGTGGCAGGCCATGAGCATCCTCTACCCg                                                           >  6:69346/1‑148 (MQ=255)                                                                                        ccGTGTCGCCGCCTGCCAGCGCCAGTTGCTGGTCGATTCGCGCCTGCAGCCGGGCCACGGCGGCAGACAGCTCGTCACTGCGCCCCGGCGCTTCACCCAGCCAGAACGGAATGGTCGGTGGCAGGCCATGAGCATCCTCTACCCGCAcc                                                       >  4:65005/1‑149 (MQ=255)                                                                                         cGTGTCGCCGCCTGCCAGCGCCAGTTGCTGGTCGATTCGCGCCTGCAGCCGGGCCACGGCGGCAGACAGCTCGTCACTGCGCCCCGGCGCTTCACCCAGCCAGAACGGAATGGTCGGTGGCAGGCCATGAGCATCCTCTACCCGCAccc                                                      <  1:48018/149‑1 (MQ=255)                                                                                          gtgtCGCCGCCTGCCAGCGCCAGTTGCTGGTCGATTCGCGCCTGCAGCCGGGCCACGGCGGCAGACAGCTCGTCACTGCGCCCCGGCGCTTCACCCAGCCAGAACGGAATGGTCg                                                                                       >  3:106601/1‑115 (MQ=255)                                                                                          gtgtCGCCGCCTGCCAGCGCCAGTTGCTGGTCGATTCGCGCCTGCAGCCGGGCCACGGCGGCAGACAGCTCGTCACTGCGCCCCGGCGCTTCACCCAGCCAGAACGGAATGGTCg                                                                                       <  4:106601/115‑1 (MQ=255)                                                                                                    cTGCCAGCGCCAGTTGCTGGTCGATTCGCGCCTGCAGCCGGGCCACGGCGGCAGACAGCTCGTCACTGCGCCCCGGCGCTTCACCCAGCCAGAACGGAATGGTCGGTGGCAGGCCATGAGCATCCTCTACCCGCACCCGGCCCGGCTc                                            >  3:86252/1‑148 (MQ=255)                                                                                                         aGCGCCAGTTGCTGGTCGATTCGCGCCTGCAGCCGGGCCACGGCGGCAGACAGCTCGTCACTGCGCCCCGGCGCTTCACCCAGCCAGAACGGAATGGTCGGTGGCAg                                                                                >  4:252369/1‑107 (MQ=255)                                                                                                         aGCGCCAGTTGCTGGTCGATTCGCGCCTGCAGCCGGGCCACGGCGGCAGACAGCTCGTCACTGCGCCCCGGCGCTTCACCCAGCCAGAACGGAATGGTCGGTGGCAg                                                                                <  3:252369/107‑1 (MQ=255)                                                                                                            gcCAGTTGCTGGTCGATTCGCGCCTGCAGCCGGGCCACGGCGGCAGACAGCTCGTCACTGCGCCCCGGCGCTTCACCCAGCCAGAACGGAATGGTCGGTGGCAGGCCATGAGCATCCTCTACCCGCACCCGGCCCGGCTCGAcgcg                                      >  3:260651/1‑146 (MQ=255)                                                                                                                    cTGGTCGATTCGCGCCTGCAGCCGGGCCACGGCGGCAGACAGCTCGTCACTGCGCCCCGGCGCTTCACCCAGCCAGAACGGAATGGTCGGTGGCAGGCCATGAGCATCCTCTACCCGCACCCGGCCCGGCTCGACGCGCAGAATGCGa                            <  5:251988/148‑1 (MQ=255)                                                                                                                      ggTCGATTCGCGCCTGCAGCCGGGCCACGGCGGCAGACAGCTCTTCACTGCGCCCCGGCGCTTCACCCAGCCAGAACGGAATGGTCGGTGGCAGGCCATGAGCATCCTCTACCCGCACCCGGCCCGGCTCGACGCGCAGAATGCGATag                         >  4:11538/1‑149 (MQ=255)                                                                                                                           aTTCGCGCCTGCAGCCGGGCCACGGCGGCAGACAGCTCGTCACTGCGCCCCGGCGCTTCACCCAGCCAGAACGGAATGGTCGGTGGCAGGCCATGAGCATCCTCTACCCGCACCCGGCCCGGCTCGACGCGCAGAATGCGATAGGAGGc                    >  2:245547/1‑149 (MQ=255)                                                                                                                            ttCGCGCCTGCAGCCGGGCCACGGCGGCAGACAGCTCGTCACTGCGCCCCGGCGCTTCACCCAGCCAGAACGGAATGGTCGGTGGCAGGCCATGAGCATCCCCTACCCGCACCCGGCCCGGCTCGACGCGCAGAATGCGATAGGAGGCg                   >  7:91243/1‑149 (MQ=255)                                                                                                                               gcgcCTGCAGCCGGGCCACGGCGGCAGACAGCTCGTCACTGCGCCCCGGCGCTTCACCCAGCCAGAACGGAATGGTCGGTGGCAGGCCATGAGCATCCTCTAccc                                                            <  4:156459/105‑1 (MQ=255)                                                                                                                               gcgcCTGCAGCCGGGCCACGGCGGCAGACAGCTCGTCACTGCGCCCCGGCGCTTCACCCAGCCAGAACGGAATGGTCGGTGGCAGGCCATGAGCATCCTCTAccc                                                            >  3:156459/1‑105 (MQ=255)                                                                                                                               gcgcCTGCAGCCGGGCCACGGCGGCAGACAGCTCGTCACTGCGCCCCGGCGCTTCACCCAGCCAGAACGGAATGGTCGGTGGCAGGCCATGAGCATCCTCTACCCGCACCCGGCCCGGCTCGACGCGCAGAATGCGATAGGAGGCGtt                 <  4:168262/148‑1 (MQ=255)                                                                                                                               gcgcCTGCAGCCGGGCCACGGCGGCAGACAGCTCGTCACTGCGCCCCGGCGCTTCACCCAGCCAGAACGGAATGGTCGGTGGCAGGCCATGAGCATCCTCTACCCGCACCCGGCCCGGCTCGACGCGCAGAATGCGATAGGAGGCGTTg                >  5:110563/1‑149 (MQ=255)                                                                                                                               gcgcCTGCAGCCGGGCCACGGCGGCAGACAGCTCGTCACTGCGCCCCGGCGCTTCACCCAGCCAGAACGGAATGGTCGGTGGCAGGCCATGAGCATCCTCTACCCGCACCCGGCCCGGCTCGACGCGCAGAATGCGATAGGAGGCGTTg                <  5:69346/149‑1 (MQ=255)                                                                                                                                tgtcTGCCGCCGTGGCCCGGCTGCAGACAGCTCGTCACTGCGCCCCGGCGCTTCACCCAGCCAGAACGGAATGGTCGGTGGCAGGCCATGAGCATCCTCTACCCGCACCCGGCCCGGCTCGACGCGCAGAATGCGATAGGAGGCGTTGc               >  1:106888/4‑149 (MQ=255)                                                                                                                                 gcCTGCAGCCGGGCCACGGCGGCAGACAGCTCGTCACTGCGCCCCGGCGCTTCACCCAGCCAGAACGGAATGGTCGGTGGCAGGCCATGAGCATCCTCTACCCGCACCCGGCCCGGCTCGACGCGCAGAATGCGATAGGAGGCGTTGcc              <  2:140179/149‑1 (MQ=255)                                                                                                                                  ccTGCAGCCGGGCCACGGCGGCAGACAGCTCGTCACTGCGCCCCGGCGCTTCACCCAGCCAGAACGGAATGGTCGGTGGCAGGCCATGAGCATCCTCTACCCGCACCCGGCCCGGCTCGACGCGCAGAATGCGATAGGAGGCGTTGccc             <  3:33183/149‑1 (MQ=255)                                                                                                                                  ccTGCAGCCGGGCCACGGCGGCAGACAGCTCGTCACTGCGCCCCGGCGCTTCACCCAGCCAGAACGGAATGGTCGGTGGCAGGCCATGAGCATCCTCTACCCGCACCCGGCCCGGCTCGACGCGCAGAATGCGATAGGAGGCGTTGccc             <  4:260651/149‑1 (MQ=255)                                                                                                                                      cAGCCGGGCCACGGCGGCAGACAGCTCGTCACTGCGCCCCGGCGCTTCACCCAGCCAGAACGGAATGGTCGGTGGCAGGCCATGAGCATCCTCTACCCGCACCCGGCCCGGCTCGACGCGCAGAATGCGATAGGAGGCGTTGCCCAg           <  7:205992/147‑1 (MQ=255)                                                                                                                                      cAGCCGGGCCACGGCGGCAGACAGCTCGTCACTGCGCCCCGGCGCTTCACCCAGCCAGAACGGAATGGTCGGTGGCAGGCCATGAGCATCCTCTACCCGCACCCGGCCCGGCTCGACGCGCAGAATGCGATAGGAGGCGTTGCCCAGCt         >  4:87539/1‑149 (MQ=255)                                                                                                                                      cAGCCGGGCCACGGCGGCAGACAGCTCGTCACTGCGCCCCGGCGCTTCACCCAGCCAGAACGGAATGGTCGGTGGCAGGCCATGAGCATCCTCTACCCGCACCCGGCCCGGCTCGACGCGCAGAATGCGATAGGAGGCGTTGCCCAGCt         <  1:245547/149‑1 (MQ=255)                                                                                                                                           gggCCACGGCGGCAGACAGCTCGTCACTGCGCCCCGGCGCTTCACCCAGCCAGAACGGAATGGTCGGTGGCAGGCCATGAGCATCCTCTACCCGCACCCGGCCCGGCTCGACGCGCAGAATGCGATAGGAGGCGTTGCCCAGCTGGaaa    <  3:87539/149‑1 (MQ=255)                                                                                                                                             gCCACGGCGGCAGACAGCTCGTCACTGCGCCCCGGCGCTTCACCCAGCCAGAACGGAATGGTCGGTGGCAGGCCATGAGCATCCTCTACCCGCACCCGGCCCGGCTCGACGCGCAGAATGCGATAGGAGGCGTTGCCCAGCTGGAAAat  <  3:65005/149‑1 (MQ=255)                                                                                                                                                |                                                                                                                                                  ACGGGTGCGGGCCAGGTAGTCGAGCAAC‑TGGCCCGCACAAGCTTCGTCCAGTTCGAAGGTCGCCTGCAACCAGGCCAGTACCTCCACCGTGTCGCCGCCTGCCAGCGCCAGTTGCTGGTCGATTCGCGCCTGCAGCCGGGCCACGGCGGCAGACAGCTCGTCACTGCGCCCCGGCGCTTCACCCAGCCAGAACGGAATGGTCGGTGGCAGGCCATGAGCATCCTCTACCCGCACCCGGCCCGGCTCGACGCGCAGAATGCGATAGGAGGCGTTGCCCAGCTGGAAAAT  >  NC_002947/1215069‑1215356

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑