Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,273,757 A→G 6.9% I110T (ATC→ACC)  PP_1115 ← lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,273,7570AG 6.9% 170.0 / 2.5 58I110T (ATC→ACC) PP_1115lipoprotein
Reads supporting (aligned to +/- strand):  ref base A (27/27);  new base G (2/2);  total (29/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.92e-01

TCACCACTGCGCGAGATGTGCAGGTCCCGATGGATGAGCAACCCGGTCGCCAGTTCGCCCTGCAGGGAGTAACGCAGCGGCTGCTTGCTTCTGAGCAGCTTGGCCAGTGGCTTCAGGTGCTGCCACAGGTTGGTTCGTGCGGTGATCTTGAAAGTGCGCCGTGCATGCCCGCCCACGCTGCGCCATACGCTGGCTTCATCCTGCACCAGTAGCAGGTCATTGAGGCGTATTGCATACGACACGTTGCGAATGAACAGGCGGCTGTCATTGGGGTTGTCGACCCGCAG  >  NC_002947/1273613‑1273899
                                                                                                                                                |                                                                                                                                              
tcACCACTGCGCGAGATGTGCAGGTCCCGATGGATGAGCAACCCGGTCGCCAGTTCGCCCTGCAGGGAGTAACGCAGCGGCTGCTTGCTTCTGAGCAGCTTGGCCAGTGGCTTCAGGTGCTGCCACAGGTTGGTTCGTGCGGTGATCtt                                                                                                                                            >  7:219448/1‑149 (MQ=255)
tcACCACTGCGCGAGATGTGCAGGTCCCGATGGATGAGCAACCCGGTCGCCAGTTCGCCCTGCAGGGAGTAACGCAGCGGCTGCTTGCTTCTGAGCAGCTTGGCCAGTGGCTTCAGGTGCTGCCACAGGTTGGTTCGTGCGGTGATCtt                                                                                                                                            >  6:71213/1‑149 (MQ=255)
  accacTGCGCGAGATGTGCAGGTCCCGATGGATGAGCAACCCGGTCGCCAGTTCGCCCTGCAGGGAGTAACGCAGCGGCTGCTTGCTTCTGAGCAGCTTGGCCAGTGGCTTCAGGTGCTGCCACAGGTTGGTTCGTGCGGTGATCTTGa                                                                                                                                          <  6:81173/149‑1 (MQ=255)
    cacTGCGCGAGATGTGCAGGTCCCGATGGATGAGCAACCCGGTCGCCAGTTCGCCCTGCAGGGAGTAACGCAGCGGCTGCTTGCTTCTGAGCAGCTTGGCCAGTGGCTTCAGGTGCTGCCACAGGTTGGTTCGTGCGGTGATCTTGaaa                                                                                                                                        <  4:87989/149‑1 (MQ=255)
      cTGCGCGAGATGTGCAGGTCCCGATGGATGAGCAACCCGGTCGCCAGTTCGCCCTGCAGGGAGTAACGCAGCGGCTGCTTGCTTCTGAGCAGCTTGGCCAGTGGCTTCAGGTGCTGCCACAGGTTGGTTCGTGCGGTGATCTTGAAAGt                                                                                                                                      >  8:177772/1‑149 (MQ=255)
          gcgAGATGTGCAGGTCCCGATGGATGAGCAACCCGGTCGCCAGTTCGCCCTGCAGGGAGTAACGCAGCGGCTGCTTGCTTCTGAGCAGCTTGGCCAGTGGCTTCAGGTGCTGCCACAGGTTGGTTCGTGCGGTGATCTTGAAAGTgcgc                                                                                                                                  >  4:247541/1‑149 (MQ=255)
                  tgCAGGTCCCGATGGATGAGCAACCCGGTCGCCAGTTCGCCCTGCAGGGAGTAACGCAGCGGCTGCTTGCTTCTGAGCAGCTTGGCCAGTGGCTTCAGGTGCTGCCACAGGTTGGTTCGTGCGGTGATCTTGAAAGTGCGCCGTGCATg                                                                                                                          >  3:148780/1‑149 (MQ=255)
                   gCAGGTCCCGATGGATGAGCAACCCGGTCGCCAGTTCGCCCTGCAGGGAGTAACGCAGCGGCTGCTTGCTTCTGAGCAGCTTGGCCAGTGGCTTCAGGTGCTGCCACAGGTTGGTTCGTGCGGTGATCTTGAAAGTGCGCCGTGCATgc                                                                                                                         >  6:136728/1‑149 (MQ=255)
                    cAGGTCCCGATGGATGAGCAACCCGGTCGCCAGTTCGCCCTGCAGGGAGTAACGCAGCGGCTGCTTGCTTCTGAGCAGCTTGGCCAGTGGCTTCAGGTGCTGCCACAGGTTGGTTCGTGCGGTGATCTTGAAAGTGCGCCGTGCATgcc                                                                                                                        >  6:183685/1‑149 (MQ=255)
                    cAGGTCCCGATGGATGAGCAACCCGGTCGCCAGTTCGCCCTGCAGGGAGTAACGCAGCGGCTGCTTGCTTCTGAGCAGCTTGGCCAGTGGCTTCAGGTGCTGCCACAGGTTGGTTCGTGCGGTGATCTTGAAAGTGCGCCGTGCATgcc                                                                                                                        >  8:57950/1‑149 (MQ=255)
                    cAGGTCCCGATGGATGAGCAACCCGGTCGCCAGTTCGCCCTGCAGGGAGTAACGCAGCGGCTGCTTGCTTCTGAGCAGCTTGGCCAGTGGCTTCAGGTGCTGCCACAGGTTGGTTCGTGCGGTGATCTTGAAAGTGCGCCGTGCATgc                                                                                                                         <  3:247541/148‑1 (MQ=255)
                    cAGGTCCCGATGGATGAGCAACCCGGTCGCCAGTTCGCCCTGCAGGGAGTAACGCAGCGGCTGCTTGCTTCTGAGCAGCTCGGCCAGTGGCTTCAGGTGCTGCCACAGGTTGGTTCGTGCGGTGATCTTGAAAGTGCGCCGTGCATgc                                                                                                                         <  4:37584/148‑1 (MQ=255)
                     aGGTCCCGCTGGAGGCGCAAGCCGGTCGCCAGTTCGCCCTGCAGGGAGTAACGCAGCGGCTGCTTGCTGCTGAGCAGCTTGGCCAGTGGCTTCAGGTGCTGCCACAGGTTGGTTCGTGCGGTGATCTTGAAAGTGCGCCGTGCATgccc                                                                                                                       <  8:182169/149‑1 (MQ=255)
                       gTCCCGATGGATGAGCAACCCGGTCGCCAGTTCGCCCTGCAGGGAGTAACGCAGCGGCTGCTTGCTTCTGAGCAGCTTGGCCAGTGGCTTCAGGTGCTGCCACAGGTTGGTTCGTGCGGTGATCTTGAAAGTGCGCCGTGCATgcccgc                                                                                                                     <  8:219448/149‑1 (MQ=255)
                          ccGATGGATGAGCAACCCGGTCGCCAGTTCGCCCTGCAGGGAGTAACGCAGCGGCTGCTTGCTTCTGAGCAGCTTGGCCAGTGGCTTCAGGTGCTGCCACAGGTTGGTTCGTGCGGTGATCTTGaaa                                                                                                                                        <  5:238186/127‑1 (MQ=255)
                          ccGATGGATGAGCAACCCGGTCGCCAGTTCGCCCTGCAGGGAGTAACGCAGCGGCTGCTTGCTTCTGAGCAGCTTGGCCAGTGGCTTCAGGTGCTGCCACAGGTTGGTTCGTGCGGTGATCTTGaaa                                                                                                                                        >  6:238186/1‑127 (MQ=255)
                          ccGATGGATGAGCAACCCGGTCGCCAGTTCGCCCTGCAGGGAGTAACGCAGCGGCTGCTTGCTTCTGAGCAGCTTGGCCAGTGGCTTCAGGTGCTGCCACAGGTTGGTTCGTGCGGTGATCTTGAAAGTGCGCCGTGCATGCCCGCCCa                                                                                                                  >  8:236215/1‑149 (MQ=255)
                                      cAACCCGGTCGCCAGTTCGCCCTGCAGGGAGTAACGCAGCGGCTGCTTGCTTCTGAGCAGCTTGGCCAGTGGCTTCAGGTGCTGCCACAGGTTGGTTCGTGCGGTGATCTTGAAAGTGCGCCGTGCATGCCCGCCCACGCTGCGCCATa                                                                                                      <  7:236215/149‑1 (MQ=255)
                                        aCCCGGTCGCCAGTTCGCCCTGCAGGGAGTAACGCAGCGGCTGCTTGCTTCTGAGCAGCTTGGCCAGTGGCTTCAGGTGCTGCCACAGGTTGGTTCGTGCGGTGATCTTGAAAGTGCGCCGTGc                                                                                                                             >  8:156577/1‑124 (MQ=255)
                                        aCCCGGTCGCCAGTTCGCCCTGCAGGGAGTAACGCAGCGGCTGCTTGCTTCTGAGCAGCTTGGCCAGTGGCTTCAGGTGCTGCCACAGGTTGGTTCGTGCGGTGATCTTGAAAGTGCGCCGTGc                                                                                                                             <  7:156577/124‑1 (MQ=255)
                                         cccGGTCGCCAGTTCGCCCTGCAGGGAGTAACGCAGCGGCTGCTTGCTTCTGAGCAGCTTGGCCAGTGGCTTCAGGTGCTGCCACAGGTTGGTTCGTGCGGTGATCTTGAAAGTGCGCCGTGc                                                                                                                             <  3:215293/123‑1 (MQ=255)
                                         cccGGTCGCCAGTTCGCCCTGCAGGGAGTAACGCAGCGGCTGCTTGCTTCTGAGCAGCTTGGCCAGTGGCTTCAGGTGCTGCCACAGGTTGGTTCGTGCGGTGATCTTGAAAGTGCGCCGTGc                                                                                                                             >  4:215293/1‑123 (MQ=255)
                                         cccGGTCGCCAGTTCGCCCTGCAGGGAGTAACGCAGCGGCTGCTTGCTTCTGAGCAGCTTGGCCAGTGGCTTCAGGTGCTGCCACAGGTTGGTTCGTGCGGTGATCTTGAAAGTGCGCCGTGCATGCCCGCCCACGCTGCGCCATACGc                                                                                                   >  5:245292/1‑149 (MQ=255)
                                           cGGTCGCCAGTTCGCCCTGCAGGGAGTAACGCAGCGGCTGCTTGCTTCTGAGCAGCTTGGCCAGTGGCTTCAGGTGCTGCCACAGGTTGGTTCGTGCGGTGATCTTGAAAGTGCGCCGTGCATGCCCGCCCACGCTGCGCCATACGCTg                                                                                                 <  5:183685/149‑1 (MQ=255)
                                             gTCGCCAGTTCGCCCTGCAGGGAGTAACGCAGCGGCTGCTTGCTTCTGAGCAGCTTGGCCAGTGGCTTCAGGTGCTGCCACAGGTTGGTTCGTGCGGTGATCTTGAAAGTGCGCCGTGCATGCCCGCCCACGCTGCGCCATACGCTGGc                                                                                               >  7:86528/1‑149 (MQ=255)
                                                gCCAGTTCGCCCTGCAGGGAGTAACGCAGCGGCTGCTTGCTTCTGAGCAGCTTGGCCAGTGGCTTCAGGTGCTGCCACAGGTTGGTTCGTGCGGTGATCTTGAAAGTGCGCCGTGCATGCCCGCCCACGCTGCGCCATACGCTGGCTTc                                                                                            <  4:148780/149‑1 (MQ=255)
                                                                   aGTAACGCAGCGGCTGCTTGCTTCTGAGCAGCTTGGCCAGTGGCTTCAGGTGCTGCCACAGGTTGGTTCGTGCGGTGATCTTGAAAGTGCGCCGTGCATGCCCGCCCACGCTGCGCCATACGCTGGCTTCATCCTGCACCAGTAGCAgg                                                                         >  1:246649/1‑149 (MQ=255)
                                                                     tAACGCAGCGGCTGCTTGCTTCTGAGCAGCTTGGCCAGTGGCTTCAGGTGCTGCCACAGGTTGGTTCGTGCGGTGATCTTGAAAGTGCGCCGTGCATGCCCGCCCACGCTGCGCCATACGCTGGCTTCATCCTGCACCAGTAGCAGGt                                                                        >  3:158689/1‑148 (MQ=255)
                                                                           aGCGGCTGCTTGCTTCTGAGCAGCTTGGCCAGTGGCTTCAGGTGCTGCCACAGGTTGGTTCGTGCGGTGATCTTGAAAGTGCGCCGTGCATGCCCGCCCACGCTGCGCCATACGCTGGCTTCATCCTGCACCAGTAGCAGGTCATTGAg                                                                 <  7:177772/149‑1 (MQ=255)
                                                                                   cttgcttCTGAGCAGCTTGGCCAGTGGCTTCAGGTGCTGCCACAGGTTGGTTCGTGCGGTGATCTTGAAAGTGCGCCGTGCATGCCCGCCCACGCTGCGCCATACGCTGGCTTCATCCTGCACCAGTAGCAGGTCATTGAGGCGTATTg                                                         <  7:57950/149‑1 (MQ=255)
                                                                                    ttgcttCTGAGCAGCTTGGCCAGTGGCTTCAGGTGCTGCCACAGGTTGGTTCGTGCGGTGATCTTGAAAGTGCGCCGTGCATGCCCGCCCACGCTGCGCCATACGCt                                                                                                  >  1:135488/1‑107 (MQ=255)
                                                                                    ttgcttCTGAGCAGCTTGGCCAGTGGCTTCAGGTGCTGCCACAGGTTGGTTCGTGCGGTGATCTTGAAAGTGCGCCGTGCATGCCCGCCCACGCTGCGCCATACGCt                                                                                                  <  2:135488/107‑1 (MQ=255)
                                                                                    ttgcttCTGAGCAGCTTGGCCAGTGGCTTCAGGTGCTGCCACAGGTTGGTTCGTGCGGTGATCTTGAAAGTGCGCCGTGCATGCCCGCCCACGCTGCGCCATACGCTGGCTTCATCCTGCACCAGTAGCAGGTCATTGAGGCGTATTg                                                         >  3:16508/1‑148 (MQ=255)
                                                                                       cttCTGAGCAGCTTGGCCAGTGGCTTCATGTTCTGCCACAGGTTGGTTCGTGCGGTGATCTTGAAAGTGCGCCGTGCATGCCCGCCCACGCTGCGCCATACGCTGGCTTCATCCTGCACCAGTAGCAGGTCATTGAGGCGTATTGCATa                                                     <  3:177812/149‑1 (MQ=255)
                                                                                               cagcTTGGCCAGTGGCTTCAGGTGCTGCCACAGGTTGGTTCGTGCGGTGATCTTGAAAGTGCGCCGTGCATGCCCGCCCACGCTGCGCCATACGCTGGCTTCATCCTGCACCAGTAGCAGGTCATTGAgg                                                                <  4:132232/130‑1 (MQ=255)
                                                                                               cagcTTGGCCAGTGGCTTCAGGTGCTGCCACAGGTTGGTTCGTGCGGTGATCTTGAAAGTGCGCCGTGCATGCCCGCCCACGCTGCGCCATACGCTGGCTTCATCCTGCACCAGTAGCAGGTCATTGAgg                                                                >  3:132232/1‑130 (MQ=255)
                                                                                                 gcTTGGCCAGTGGCTTCAGGTGCTGCCACAGGTTGGTTCGTGCGGTGATCTTGAAAGTGCGCCGTGCATGCCCGCCCACGCTGCGCCATACGCTGGCTTCATCCTGCACCAGTAGCAGGTCATTGAGGCGTATTGCATACGACACGTTg                                           <  5:71213/149‑1 (MQ=255)
                                                                                                  cTTGGCCAGTGGCTTCAGGTGCTGCCACAGGTTGGTTCGTGCGGTGATCTTGAAAGTGCGCCGTGCATGCCCGCCCACGCTGCGCCATACGCTGGCTTCATCCTGCACCAGTAGCAGGTCATTGAGGCGTATTGCATACGACACGTTGc                                          <  4:158689/149‑1 (MQ=255)
                                                                                                     ggCCAGTGGCTTCAGGTGCTGCCACAGGTTGGTTCGTGCGGTGATCTTGAAAGTGCGCCGTGCATGCCCGCCCACGCTGCGCCATACGCTGGCTTCATCCTGCACCAGTAGCAGGTCATTGAGGCGTAtt                                                          <  4:235732/130‑1 (MQ=255)
                                                                                                     ggCCAGTGGCTTCAGGTGCTGCCACAGGTTGGTTCGTGCGGTGATCTTGAAAGTGCGCCGTGCATGCCCGCCCACGCTGCGCCATACGCTGGCTTCATCCTGCACCAGTAGCAGGTCATTGAGGCGTAtt                                                          >  3:235732/1‑130 (MQ=255)
                                                                                                      gCCAGTGGCTTCAGGTGCTGCCACAGGTTGGTTCGTGCGGTGATCTTGAAAGTGCGCCGTGCATGCCCGCCCACGCTGCGCCATACGCTGGCTTCATCCTGCACCAGTAGCAGGTCATTGAGGCGTATTGCATACGACACGTTGCGAAt                                      <  5:136728/149‑1 (MQ=255)
                                                                                                          gTGGCTTCAGGTGCTGCCACAGGTTGGTTCGTGCGGTGATCTTGAAAGTGCGCCGTGCATGCCCGCCCACGCTGCGCCATACGCTGGCTTCATCCTGCACCAGTAGCAGGTCATTGAGGCGTATTGCATACGACACGTTGCGAATGaa                                   >  5:112108/1‑148 (MQ=255)
                                                                                                               ttCAGGTGCTGCCACAGGTTGGTTCGTGCGGTGATCTTGAAAGTGCGCCGTGCATGCCCGCCCACGCTGCGCCATACGCTGGCTTCATCCTGCACCAGTAGCAGGTCATTGAGGCGTATTGCATACGACACGTTGCGAATGAACAggcg                             >  5:255434/1‑149 (MQ=255)
                                                                                                                  aGGTGCTGCCACAGGTTGGTTCGTGCGGTGATCTTGAAAGTGCGCCGTGTATGCCCGCCCACGCTGCGCCATACGCTGGCTTCATCCTGCACCAGTAGCAGGTCATTGAGGCGTATTGCATACGACACGTTGCGAATGAACAGGCGGCt                          >  6:50740/1‑149 (MQ=255)
                                                                                                                          ccACAGGTTGGTTCGTGCGGTGATCTTGAAAGTGCGCCGTGCATGCCCGCCCACGCTGCGCCATACGCTGGCTTCATCCTGCACCAGTAGCAGGTCATTGAGGCGTATTGCATACGACACGTTGCGAATGAACGGGCGGCTGTCATTgg                  <  8:86528/149‑1 (MQ=255)
                                                                                                                          ccACAGGTTGGTTCGTGCGGTGATCTTGAAAGTGCGCCGTGCATGCCCGCCCACGCTGCGCCATACGCTGGCTTCATCCTGCACCAGTAGCAGGTCATTGAGGCGTATTGCATACGACACGTTGCGAATGAACAGGCGGCTGTCAtt                    <  6:245292/147‑1 (MQ=255)
                                                                                                                          ccACAGGTTGGTTCGTGCGGTGATCTTGAAAGTGCGCCGTGCATGCCCGCCCACGCTGCGCCATACGCTGGCTTCATCCTGCACCAGTAGCAGGTCATTGAGGCGTATTGCATACGACACGTTGCGAATGAACAGGCGGCTGTCATTgg                  >  1:141585/1‑149 (MQ=255)
                                                                                                                          ccACAGGTTGGTTCGTGCGGTGATCTTGAAAGTGCGCCGTGCATGCCCGCCCACGCTGCGCCATACGCTGGCTTAATCCTGCCCCAGTAGCAGCTCATTGAGGCGTATTGCATACGACACGTTGCGAATCAACAGGCCGCTGTCATTgg                  >  6:202546/1‑149 (MQ=255)
                                                                                                                            acaGGTTGGTTCGTGCGGTGATCTTGAAAGTGCGCCGTGCATGCCCGCCCACGCTGCGCCATACGCTGGCTTCATCCTGCACCAGTAGCAGGTCATTGAGGCGTATTGCATACGACACGTTGCGAAt                                      >  4:260554/1‑127 (MQ=255)
                                                                                                                            acaGGTTGGTTCGTGCGGTGATCTTGAAAGTGCGCCGTGCATGCCCGCCCACGCTGCGCCATACGCTGGCTTCATCCTGCACCAGTAGCAGGTCATTGAGGCGTATTGCATACGACACGTTGCGAAt                                      <  3:260554/127‑1 (MQ=255)
                                                                                                                              aGGTTGGTTCGTGCGGTGATCTTGAAAGTGCGCCGTGTATGCCCGCCCACGCTGCGCCATACGCTGGCTTCATCCTGCACCAGTAGCAGGTCATTGAGGCGTATTGCATACGACACGTTGCGAATGAACAGGCGGCTGTCATTGGGGtt              <  5:50740/149‑1 (MQ=255)
                                                                                                                                gttggttCGTGCGGTGGTCTTGAAAGTGCGCCGTGCATGCCCGCCCACGCTGCGCCATACGCTGGCTTCATCCTg                                                                                      >  7:79269/1‑75 (MQ=255)
                                                                                                                                gttggttCGTGCGGTGGTCTTGAAAGTGCGCCGTGCATGCCCGCCCACGCTGCGCCATACGCTGGCTTCATCCTg                                                                                      >  7:75478/1‑75 (MQ=255)
                                                                                                                                gttggttCGTGCGGTGGTCTTGAAAGTGCGCCGTGCATGCCCGCCCACGCTGCGCCATACGCTGGCTTCATCCTg                                                                                      <  8:75478/75‑1 (MQ=255)
                                                                                                                                gttggttCGTGCGGTGGTCTTGAAAGTGCGCCGTGCATGCCCGCCCACGCTGCGCCATACGCTGGCTTCATCCTg                                                                                      <  8:79269/75‑1 (MQ=255)
                                                                                                                                    gttCGTGCGGTGATCTTGAAAGTGCGCCGTGCATGCCCGCCCACGCTGCGCCAGACGCTGTCTTCATCCTGCACCAGTAGCAGGTCATTGAGGCGTATAGCATACGACACGTTGCGAATGAACAGGCGGCTGTCATTGGGGTTGTCGa         <  5:202546/148‑1 (MQ=255)
                                                                                                                                            ggTGATCTTGAAAGTGCGCCGTGCATGCCCGCCCACGCTGCGCCATACGCTGGCTTCATCCTGCACCAGTAGCAGGTCATTGAGGCGTATTGCATACGACACGTTGCGAATGAACAGGCGGCTGTCATTGGGGTTGTCGACCCGCAg  <  6:74465/147‑1 (MQ=255)
                                                                                                                                            ggTGATCTTGAAAGTGCGCCGTGCATGCCCGCCCACGCTGCGCCATACGCTGGCTTCATCCTGCACCAGTAGCAGGTCATTGAGGCGTATTGCATACGACACGTTGCGAATGAACAGGCGGCTGTCATTGGGGTTGTCGACCCGCAg  >  5:74465/1‑147 (MQ=255)
                                                                                                                                                |                                                                                                                                              
TCACCACTGCGCGAGATGTGCAGGTCCCGATGGATGAGCAACCCGGTCGCCAGTTCGCCCTGCAGGGAGTAACGCAGCGGCTGCTTGCTTCTGAGCAGCTTGGCCAGTGGCTTCAGGTGCTGCCACAGGTTGGTTCGTGCGGTGATCTTGAAAGTGCGCCGTGCATGCCCGCCCACGCTGCGCCATACGCTGGCTTCATCCTGCACCAGTAGCAGGTCATTGAGGCGTATTGCATACGACACGTTGCGAATGAACAGGCGGCTGTCATTGGGGTTGTCGACCCGCAG  >  NC_002947/1273613‑1273899

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑