Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,293,369 T→C 10.5% P324P (CCT→CCC)  PP_1128 → OmpA family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,293,3690TC10.5% 97.8 / 3.2 38P324P (CCT→CCC) PP_1128OmpA family protein
Reads supporting (aligned to +/- strand):  ref base T (17/17);  new base C (2/2);  total (19/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.00e-01

GATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCC‑AAAACGCCTGCGAAGACTGCAGCCAAGAAAAAAGCTGCGCCGGCCAAGCCTGCCGCCAAGAAAGCTGCCGTTGACAAGAAAGTAGCTGCCAACAGCCCTGCGAAGACCAACTGATCGTCAAGGAACCGCAGTAATGACCCAATCCCTGGCCGATATGCGCCGCGACTACACCCGTGATGGCCTGGCCGAAGACCAGGCA  >  NC_002947/1293222‑1293517
                                                                                                                                                    |                                                                                                                                                    
gATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCC‑AAAACGCCTGCGAAGACTGCAGCCAAGAAAAAAGCTGCGCCGGCCAAGCCTg                                                                                                                                                     <  6:174773/149‑1 (MQ=255)
 aTGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCC‑AAAACGCCTGCGAAGACTGCAGCCAAGAAAAAAGCTGCGCCGGCCAAGCCTgc                                                                                                                                                    <  8:80742/149‑1 (MQ=255)
         ggTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGTCC‑AAAACGCCTGCGAAGACTGCAGCCAAGAAAAAAGCTGCGCCGGCCAAGCCTGCCGCCAAGa                                                                                                                                            <  2:178573/149‑1 (MQ=255)
          gTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCC‑AAAACGCCTGCGAAGACTGCAGCCAAGAAAAAAGCTGCGCCGGCCAAGCCTGCCGCCAAGa                                                                                                                                            >  3:168874/1‑148 (MQ=255)
                gATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCC‑AAAACGCCTGCGAAGACTGCAGCCAAGAAAAAAGCTGCGCCGGCCAAGCCTGCCGCCAAGAAAGCTGc                                                                                                                                     >  7:36201/1‑149 (MQ=255)
                    ccAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCC‑AAAACGCCTGCGAAGACTGCAGCCAAGAAAAAAGCTGCGCCGGCCAAGCCTGCCGCCAAGAAAGCTGCCGtt                                                                                                                                 <  7:187416/149‑1 (MQ=255)
                           aCCAGCGGCCTTGGCAGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCC‑AAAACGCCTGCGAAGACTGCAGCCAAGAAAAAAGCTGCGCCGGCCAAGCCTGCCGCCAAGAAAGCTGCCGTTGACa                                                                                                                             >  7:172940/1‑146 (MQ=255)
                           aCCAGCGGCCTTGGCAGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCC‑AAAACGCCTGCGAAGACTGCAGCCAAGAAAAAAGCTGCGCCGGCCAAGCCTGCCGCCAAGAAAGCTGCCGTTGACAAg                                                                                                                           >  1:134246/1‑148 (MQ=255)
                                                  gCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCC‑AAAACGCCTGCGAAGACTGCAGCCAAGAAAAAAGCTGCGCCGGCCAAGCCTGCCGCCAAGAAAGCTGCCGTTGACAAGAAAGTAGCTGCCAACAGCCCTGc                                                                                                    >  2:145312/1‑148 (MQ=255)
                                                  gCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCC‑AAAACGCCTGCGAAGACTGCAGCCAAGAAAAAAGCTGCGCCGGCCAAGCCTGCCGCCAAGAAAGCTGCCGTTGACAAGAAAGTAGCTGCCAACAGCCCTGCg                                                                                                   <  8:36201/149‑1 (MQ=255)
                                                              tCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCC‑AAAACGCCTGCGAAGACTGCAGCCAAGAAAAAAGCTGCGCCGGCCAAGCCTGCCGCCAAGAAAGCTGCCGTTGACAAGAAAGTAGCTGCCAACAGCCCTGCGAAGACCAACTGa                                                                                       <  2:134246/149‑1 (MQ=255)
                                                                     tGCGGCGAAGAAACCTGTAGCCAAGGCCGAAAACGCCTGCGAAGACTGCAGCCAAGAAAAAAGCTGCGCCGGCCAAGCCCGCCGCCAAGAAAGCTGCCGTTGACAAGAAAGTAGCTGCCAACAGCCCTGCGaa                                                                                                 >  1:15918/1‑133 (MQ=255)
                                                                     tGCGGCGAAGAAACCTGTAGCCAAGGCC‑AAAACGCCTGCGAAGACTGCAGCCAAGAAAAAAGCTGCGCCGGCCAAGCCTGCCGCCAAGAAAGCTGCCGTTGACAAGAAAGTAGCTGCCAACAGCCCTGCGAAGACCAACTGATCGTCaa                                                                                <  3:65494/149‑1 (MQ=255)
                                                                     tGCGGCGAAGAAACCTGTAGCCAAGGCC‑AAAACGCCTGCGAAGACTGCAGCCAAGAAAAAAGCTGCGCCGGCCAAGCCCGCCGCCAAGAAAGCTGCCGTTGACAAGAAAGTAGCTGCCAACAGCCCTGCGaa                                                                                                 <  8:7385/132‑1 (MQ=255)
                                                                     tGCGGCGAAGAAACCTGTAGCCAAGGCC‑AAAACGCCTGCGAAGACTGCAGCCAAGAAAAAAGCTGCGCCGGCCAAGCCCGCCGCCAAGAAAGCTGCCGTTGACAAGAAAGTAGCTGCCAACAGCCCTGCGaa                                                                                                 >  7:7385/1‑132 (MQ=255)
                                                                     tGCGGCGAAGAAACCTGTAGCCAAGGCC‑AAAACGCCTGCGAAGACTGCAGCCAAGAAAAAAGCTGCGCCGGCCAAGCCCGCCGCCAAGAAAGCTGCCGTTGACAAGAAAGTAGCTGCCAACAGCCCTGCGaa                                                                                                 <  2:15918/132‑1 (MQ=255)
                                                                            aagaaACCTGTAGCCAAGGCC‑AAAACGCCTGCGAAGACTGCAGCCAAGAAAAAAGCTGCGCCGGCCAAGCCTGCCGCCAAGAAAGCTGCCGTTGACAAGAAAGTAGCTGCCAACAGCCCTGCGAAGACCAACTGATCGTCAAGGAACCg                                                                         >  1:101259/1‑149 (MQ=255)
                                                                              gaaACCTGTAGCCAAGGCC‑AAAACGCCTGCGAAGACTGCAGCCAAGAAAAAAGCTGCGCCGGCCAAGCCTGCCGCCAAGAAAGCTGCCGTTGACAAGAAAGTAGCTGCCAACAGCCCTGCGaa                                                                                                 <  5:273833/123‑1 (MQ=255)
                                                                              gaaACCTGTAGCCAAGGCC‑AAAACGCCTGCGAAGACTGCAGCCAAGAAAAAAGCTGCGCCGGCCAAGCCTGCCGCCAAGAAAGCTGCCGTTGACAAGAAAGTAGATGCCAACAGCCCTGCGaa                                                                                                 >  6:273833/1‑123 (MQ=255)
                                                                                   ctgtccccAAGGCC‑AAAACCCCTGCGACCACTCCAGCCAAGAAAAAAGCTGCGCCGGCCAAGCCTGCCGCCAACAAAGCTGCCGTTGACAAGAAAGTACCTGCCAACAGCCCTGCGAAGACCAACTGAGCGGCAAGGAACCGCATTAAt                                                                  <  6:11946/143‑1 (MQ=255)
                                                                                   cTGTAGCCAAGGCC‑AAAACGCCTGCGAAGACTGGAGCCAAGAAAAAAGCTGCGCCGGCCAAGCCTGCCGCCAAGAAAGCTGCCGTTGACAAGAAAGTAGCTGCCAACAGCCCTGCGAAGACCAACTGATCGTCAAGGAACCGCAGTAAt                                                                  <  7:163670/149‑1 (MQ=255)
                                                                                      tAGCCAAGGCC‑AAAACCCCTGCGAAGACTGCAGCCAAGAAAAAAGCTGCGCCGGCCAAGCCTGCCCCCAAGAACGCTGCCGATGACAAGAAAGTAGCTGCCAACCGCCCTGCGACGCCCACCGGATCGTCCAGGCACCGCCGTAAtccc                                                               >  4:186617/1‑146 (MQ=255)
                                                                                         ccAAGGCC‑AAAACGCCTGCGAAGACTGCAGCCAAGAAAAAAGCTGCGCCGGCCAAGCCTGCCGCCAAGAAAGCTGCCGTTGACAAGAAAGTAGCt                                                                                                                  >  1:124843/1‑95 (MQ=255)
                                                                                         ccAAGGCC‑AAAACGCCTGCGAAGACTGCAGCCAAGAAAAAAGCTGCGCCGGCCAAGCCTGCCGCCAAGAAAGCTGCCGTTGACAAGAAAGTAGCt                                                                                                                  <  2:124843/95‑1 (MQ=255)
                                                                                           aaGGCC‑AAAACGCCTGCGAAGACTGCAGCCAAGAAAAAAGCTGCGCCGGCCAAGCCTGCCGCCAAGAAAGCTGCCGTTGACAAGAAAGTAGCTGCCAACAGCCCTGCGAAGACCAACTGATCGTCAAGGAACCGCAGTAATGACCCAAt                                                          >  2:207914/1‑149 (MQ=255)
                                                                                              gCC‑AAAACGCCTGCGAAGACTGCAGCCAAGAAAAAAGCTGCGCCGGCCAAGCCTGCCGCCAAGAAAGCTGCCGTTGACAAGAAAGTAGCTGCCAACAGCCCTGCGAAGACCAACTGATCGTCAAGGAACCGCAGTAATGACCCAATccc                                                       >  2:49920/1‑149 (MQ=255)
                                                                                                c‑AAAACGCCTGCGAAGACTGCAGCCAAGAAAAAAGCTGCGCCGGCCAAGCCTGCCGCCAAGAAAGCTGCCGTTGACAAGAAAGTAGCTGCCAACAGCCCTGCGAAGACCAACTGATCGTCAAGGAACCGCAGTAATGACCCAATCCCTg                                                     >  5:25849/1‑149 (MQ=255)
                                                                                                c‑AAAACGCCTGCGAAGACTGCAGCCAAGAAAAAAGCTGCGCCGGCCAAGCCTGCCGCCAAGAAAGCTGCCGTTGACAAGAAAGTAGCTGCCAACAGCCCTGCGAAGACCAACTGATCGTCAAGGAACCGCAGTAATGACCCAATCCCTg                                                     >  7:190938/1‑149 (MQ=255)
                                                                                                          tGCGAAGACTGCAGCCAAGAAAAAAGCTGCGCCGGCCAAGCCTGCCGCCAAGAAAGCTGCCGTTGACAAGAAAGTAGCTGCCAACAGCCCTGCGAAGACCAACTGATCGTCAAGGAACCGCAGTAATGACCCAATCCCTGGCCGATATg                                            >  1:186852/1‑149 (MQ=255)
                                                                                                              aaGACTGCAGCCAAGAAAAAAGCTGCGCCGGCCAAGCCTGCCGCCAAGAAAGCTGCCGTTGACAAGAAAGTAGCTGCCAACAGCCCTGCGAAGACCAACTGATCGTCAAGGAACCGCAGTAATGACCCAATCCCTGGCCGATATGcgcc                                        <  1:49920/149‑1 (MQ=255)
                                                                                                                  cTGCAGCCAAGAAAAAAGCTGCGCCGGCCAAGCCTGCCGCCAAGAAAGCTGCCGTTGACAAGAAAGTAGCTGCCAACAGCCCTGCGAAGACCAACTGATCGTCAAGGAACCGCAGTAATGACCCAATCCCTGGCCGATATGCGCCGCGa                                    <  6:25849/149‑1 (MQ=255)
                                                                                                                         cAAGAAAAAAGCTGCGCCGGCCAAGCCTGCCGCCAAGAAAGCTGCCGTTGACAAGAAAGTAGCTGCCAACAGCCCTGCGAAGACCAACTGATCGTCAAGGAACCGCAGTAATGACCCAATCCCTGGCCGATATGCGCCGCGACTACAcc                             >  1:16037/1‑149 (MQ=255)
                                                                                                                               aaaaGCTGCGCCGGCCAAGCCTGCCGCCAAGAAAGCTGCCGTTGACAAGAAAGTAGCTGCCAACAGCCCTGCGAAGACCAACTGATCGTCAAGGAACCGCAGTAATGACCCAATCCCTGGCCGATATGCGCCGCGACTACACCCGTGAt                       >  2:80609/1‑149 (MQ=255)
                                                                                                                                       cgcCGGCCAAGCCTGCCGCCAAGAAAGCTGCCGTTGACAAGAAAGTAGCTGCCAACAGCCCTGCGAAGACCAACTGATCGTCAAGGAACCGCAGTAATGACCCAATCCCTGGCCGATATGCGCCGCGACTACACCCGTGAtggcctggc               <  4:168874/149‑1 (MQ=255)
                                                                                                                                       cgcCGGCCAAGCCTGCCGCCAAGAAAGCTGCCGTTGACAAGAAAGTAGCTGCCAACAGCCCTGCGAAGACCAACTGATCGTCAAGGAACCGCAGTAATGACCCAATCCCTGGCCGATATGCGCCGCGACTACACCCGTGAtggcctg                 <  2:186852/147‑1 (MQ=255)
                                                                                                                                       cgcCGGCCAAGCCTACCGCCAAGAAAGCTGCCGTTGACAAGAAAGTAGCTGCCAACAGCCCTGCGAAGACCAACTGATCGTCAAGGAACCGCAGTAATGACCCAATCCCTGGCCGATATGCGCCGCGACTACACCCGTGAtggcctgg                <  7:44076/148‑1 (MQ=255)
                                                                                                                                           ggCCAAGCCTGCCGCCAAGAAAGCTGCCGTTGACAAGAAAGTAGCTGCCAACAGCCCTGCGAAGACCAACTGATCGTCAAGGAACCGCAGTAATGACCCAATCCCTGGCCGATATGCGCCGCGACTACACCCGTGATGGCCTGGCCGaa           <  2:101259/149‑1 (MQ=255)
                                                                                                                                             ccAAGCCTGCCGCCAAGAAAGCTGCCGTTGACAAGAAAGTAGCTGCCAACAGCCCTGCGAAGACCAACTGATCGTCAAGGAACCGCAGTAATGACCCAATCCCTGGCCGATATGCGCCGCGACTACACCCGTGATGGCCTGGCCGAAGa         >  5:212524/1‑149 (MQ=255)
                                                                                                                                                    tGCCGCCAAGAAAGCTGCCGTTGACAAGAAAGTAGCTGCCAACAGCCCTGCGAAGACCAACTGATCGTCAAGGAACCGCAGTAATGACCCAATCCCTGGCCGATATGCGCCGCGACTACACCCGTGATGGCCTGGCCGAAGACCAGGCa  >  6:65920/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCC‑AAAACGCCTGCGAAGACTGCAGCCAAGAAAAAAGCTGCGCCGGCCAAGCCTGCCGCCAAGAAAGCTGCCGTTGACAAGAAAGTAGCTGCCAACAGCCCTGCGAAGACCAACTGATCGTCAAGGAACCGCAGTAATGACCCAATCCCTGGCCGATATGCGCCGCGACTACACCCGTGATGGCCTGGCCGAAGACCAGGCA  >  NC_002947/1293222‑1293517

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑