| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,318,420 | T→A | 11.1% | L613Q (CTG→CAG) | PP_1150 → | membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,318,420 | 0 | T | A | 11.1% | 93.7 / 3.7 | 36 | L613Q (CTG→CAG) | PP_1150 | membrane protein |
| Reads supporting (aligned to +/- strand): ref base T (17/15); new base A (2/2); total (19/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01 | |||||||||||
CTTCACGGGCTGGATATCGGCGACGAACTGGTGCACCTGCGCATGTGCCTGGCGGTCGCTCAGGCCCCTTTGGGGCGGGCCGAGCGCGAGTACCTGCAACACGTGGAGGCAGCGCTGGCCAAAGGGCCTGCTGCCGGGCGTGGGCAGCTGCTGGATGCTGCCAGTGAGCAGTTCATTGCGGCCTTGCGTCGGCTACCCGCCAGCGACCCGCTTCGCCTGGCCGAGGGGGCAGTACTGCAATTGCAGAAAAGCTGGGGCAAATGGTGCCGCTGGCAGGAGGACACCCATGGG > NC_002947/1318272‑1318562 | cTTCACGGGCTGGATATCGGCGACGAACTGGTGCACCTGCGCATGTGCCTGGCGGTCGCTCAGGCCCCTTTGGGGCGGGCCGAGCGCGAGTACCTGCAACACGTGGAGGCAGCGCTGGCCAAAGGGCCTGCTGCCGGGCGTGGGCAgct < 1:75511/149‑1 (MQ=255) aCGGGCTGGATATCGGCGACGAACTGGTGCACCTGCGCATGTGCCTGGCGGTCGCTCAGGCCCCTTTGGGGCGGGCCGAGCGCGAGTACCTGCAACACGTGGAGGCAGCGCTGGCCAAAGGGCCTGCTGCCGGGCGTGGGCAGctgctg < 8:171559/149‑1 (MQ=255) gCTGGATATCGGCGACGAACTGGTGCACCTGCGCATGTGCCTGGCGGTCGCTCAGGCCCCTTTGGGGCGGGTCGAGCGCGAGTACCTGCAACACGTGGAGGCAGCGCTGGCCAAAGGGCCTGCTGCCGGGCGTGGGCAGCTGCTGGAtg > 6:159272/1‑149 (MQ=255) gATATCGGCGACGAACTGGTGCACCTGCGCATGTGCCTGGCGGTCGCTCAGGCCCCTTTGGGGCGGGCCGAGCGCGAGTACCTGCAACACGTGGAGGCAGCGCTGGCCAAAGGGCCTGCTGCCGGGCGTGGGCAGCTGCTGGAtgctgc < 8:113830/149‑1 (MQ=255) tCGGCGACGAACTGGTGCACCTGCGCATGTGCCTGGCGGTCGCTCAGGCCCCTTTGGGGCGGGCCGAGCGCGAGTACCTGCAACACGTGGAGGCAGCGCTGGCCAAAGGGCCTGCTGCCGGGCGTGGGCAGCTGCTGGATGCTGCCAGt < 6:139486/149‑1 (MQ=255) tCGGCGACGAACTGGTGCACCTGCGCATGTGCCTGGCGGTCGCTCAGGCCCCTTTGGGGCGGGCCGAGCGCGAGTACCTGCAACACGTGGAGGCAGCGCTGGCCAAAGGGCCTGCTGCCGGGCGTGGGCAGCTGCTGGATGCTGCCAGt > 3:65252/1‑149 (MQ=255) gTGCACCTGCGCATGTGCCTGGCGGTCGCTCAGGCCCCTTTGGGGCGGGCCGAGCGCGAGTACCTGCAACACGTGGAGGCAGCGCTGGCCAAAGGGCCTGCTGCCGGGCGTGGGCAGCTGCTGGATGCTGCCAGTGAGCAGTTCATTGc > 8:83266/1‑149 (MQ=255) cACCTGCGCATGTGCCTGGCGGTCGCTCAGGCCCCTTTGGGGCGGGCCGAGCGCGAGTACCTGCAACACGTGGAGGCCGCGCTGGCCAAAGGGCCTGCTGCCGGGCGTGGGCAGCTGCTGGATGCTGCCAGTGAGCAGTTCATTGCgg < 7:118259/148‑1 (MQ=255) gcgcATGTGCCTGGCGGTCGCTCAGGCCCCTTTGGGGCGGGCCGAGCGCGAGTACCTGCAACACGTGGAGGCAGCGCTGGCCAAAGGGCCTGCTGCCGGGCGTGGGCAGCTGCTGGATGCTGCCAGTGAGCAGt < 3:107861/134‑1 (MQ=255) gcgcATGTGCCTGGCGGTCGCTCAGGCCCCTTTGGGGCGGGCCGAGCGCGAGTACCTGCAACACGTGGAGGCAGCGCTGGCCAAAGGGCCTGCTGCCGGGCGTGGGCAGCTGCTGGATGCTGCCAGTGAGCAGt > 4:107861/1‑134 (MQ=255) aTGTGCCTGGCGGTCGCTCAGGCCCCTTTGGGGCGGGCCGAGCGCGAGTACCTGCAACACGTGGAGGCAGCGCTGGCCAAAGGGCCTGCTGCCGGGCGTGGGCAGCTGCTGGATGCTGCCAGTGAGCAGTTCATTGCGGCCTTGCGTCg > 3:250873/1‑149 (MQ=255) ccTGGCGGTCGCTCAGGCCCCTTTGGGGCGGGCCGAGCGCGAGTACCT‑CAACACGTGGAGGCAGCGCTGGCCAAAGGGCCTGCTGCCGGGCGTGGGCAGCTGCTGGATGCTGCCAGTGAGCAGTTCATTGCGGCCTTGCGTCGGCTAcc > 2:92846/1‑149 (MQ=255) tGGCGGTCGCTCAGGCCCCTTTGGGGCGGGCCGAGCGCGAGTACCTGCAACACGTGGAGGCAGCGCTGGCCAAAGGGCCTGCTGCCGGGCGTGGGCAGCAGCTGGATGCTGCCAGTGAGCAGTTCATTGCGGCCTTGCGTCGGCTAccc > 7:121403/1‑149 (MQ=255) tGGCGGTCGCTCAGGCCCCTTTGGGGCGGGCCGAGCGCGAGTACCTGCAACACGTGGAGGCAGCGCTGGCCAAAGGGCCTGCAGCCGGGCGTGGGCAGCAGCTGGATGCTGCCAGTGAGCAGTTCATTGCGGCCTTGCGTCGGCTAccc > 7:124894/1‑149 (MQ=255) ggCCCCTTTGGGGCGGGCCGAGCGCGAGTACCTGCAACACGTGGAGGCAGCGCTGGCCAAAGGGCCTGCTGCCGGGCGTGGGCAGCTGCTGGATGCTGCCAGTGAGCAGtt > 6:245870/1‑111 (MQ=255) ggCCCCTTTGGGGCGGGCCGAGCGCGAGTACCTGCAACACGTGGAGGCAGCGCTGGCCAAAGGGCCTGCTGCCGGGCGTGGGCAGCTGCTGGATGCTGCCAGTGAGCAGtt < 5:245870/111‑1 (MQ=255) ggCCCCTTTGGGGCGGGCCGAGCGCGAGTACCTGCAACACGTGGAGGCAGCGCTGGCCAAAGGGCCTGCTGCCGGGCGTGGGCAGCAGCTGGATGCTGCCAGTGAGCAGTTCATTGCGGCCTTGCGTCGGCTACCCGCCAGCGACCCGc < 8:121403/149‑1 (MQ=255) ggCCCCTTTGGGGCGGGCCGAGCGCGAGTACCTGCAACACGTGGAGGCAGCGCTGGCCAAAGGGCCTGCTGCCGGGCGTGGGCAGCAGCTGGATGCTGCCAGTGAGCAGTTCATTGCGGCCTTGCGTCGGCTACCCGCCAGCGACCCGc < 8:124894/149‑1 (MQ=255) gCCCCTTTGGGGCGGGCCGAGCGCGAGTACCTGCAACACGTGGAGGCAGCGCTGGCCAAAGGGCCTGCTGCCGGGCGTGGGCAGCTGCTGGATGCTGCCAGTGAGCAGTTCATTGCGGCCTTGCGTCGGCTACCCGCCAGCGACCCGCt > 1:163142/1‑149 (MQ=255) cccTTTGGGGCGGGCCGAGCGCGAGTACCTGCAACACGTGGAGGCAGCGCTGGCCAAAGGGCCTGCTGCCGGGCGTGGGCAGCTGCTGGATGCTGCCAGTGAGCAGTTCATTGCGGCCTTGCGTCGGCTACCCGCCAGCGACCCGCTTc < 1:175499/149‑1 (MQ=255) ggggCGGGCCGAGCGCGAGTACCTGCAACACGTGGAGGCAGCGCTGGCCAAAGGGCCTGCTGCCGGGCGTGGGCAGCTGCTGGATGCTGCCAGTGAGCAGTTCATTGCGGCCTTGCGTCGGCTACCCGCCAGCGACCCGCTTCGCCTgg > 6:95341/1‑149 (MQ=255) gcctgAGCGCGAGTACCTGCAACACGTGGAGGCAGCGCTGGCCAAAGGGCCTGCTGCCGGGCGTGGGCAGCTGCTGGATGCTGCCAGTGAGCAGTTCATTGCGGCCTTGCGTCGGCTACCCGCCAGCGACCCGCTTCGCCTGGCCGAgg < 5:64314/145‑1 (MQ=255) ccGAGCGCGAGTACCTGCAACACCTGCAGGCAGCGCTGGCCAAAGGGCCTGCTGCCGGGCGTGGGCAGCTGCTGCATGCTGCCACTGAGCAGTTCATTGCGGCCTTTCGTCGGCTACCCGCCAGCGACCCCCATCGCCTGGCCGacgag > 8:75917/1‑145 (MQ=255) cGAGCGCGAGTACCTGCAACACGTGGAGGCAGCGCTGGCCAAAGGGCCTGCTGCCGGGCGTGGGCAGCTGCTGGATGCTGCCAGTGAGCAGTTCATTGCGGCCTTGCGTCGGCTACCCGCCAGCGACCCGCTTCGCCTGGCCGAggggg < 5:159272/149‑1 (MQ=255) aGCGCGAGTACCTGCAACACGTGGAGGCAGCGCTGGCCAAAGGGCCTGCTGCCGGGCGTGGGCAGCTGCTGGATGCTGCCAGTGAGCAGTTCATTGCGGCCTTGCGTCGGCTACCCGCCAGCGACCCGCTTc > 5:94714/1‑132 (MQ=255) aGCGCGAGTACCTGCAACACGTGGAGGCAGCGCTGGCCAAAGGGCCTGCTGCCGGGCGTGGGCAGCTGCTGGATGCTGCCAGTGAGCAGTTCATTGCGGCCTTGCGTCGGCTACCCGCCAGCGACCCGCTTc < 6:94714/132‑1 (MQ=255) aGCGCGAGTACCTGCAACACGTGGAGGCAGCGCTGGCCAAAGGGCCTGCTGCCGGGCGTGGGCAGCTGCTGGATGCTGCCAGTGAGCAGTTCATTGCGGCCTTGCGTCGGCTACCCGCCAGCGACCCGCTTCGCCTGGCCGAGGGGGCa < 2:163142/149‑1 (MQ=255) ccTGCAACACGTGGAGGCAGCGCTGGCCAAAGGGCCTGCTGCCGGGCGTGGGCAGCTGCTGGATGCTGCCAGTGAGCAGTTCATTGCGGCCTTGCGTCGGCTACCCGCCAGCGACCCGCTTCGCCTGGCCGAGGGGGCAGTACTGCaa < 4:65252/148‑1 (MQ=255) cTGCAACACGTGGAGGCAGCGCTGGCCAAAGGGCCTGCTGCCGGGCGTGGGCAGCTGCTGGATGCTGCCAGTGAGCAGTTCATTGCGGCCTTGCGTCGGCTAc < 4:60564/103‑1 (MQ=255) cTGCAACACGTGGAGGCAGCGCTGGCCAAAGGGCCTGCTGCCGGGCGTGGGCAGCTGCTGGATGCTGCCAGTGAGCAGTTCATTGCGGCCTTGCGTCGGCTAc > 3:60564/1‑103 (MQ=255) tGGAGGCAGCGCTGGCCAAAGGGCCTGCTGCCGGGCGTGGGCAGCTGCTGGATGCTGCCAGTGAGCAGTTCATTGCGGCCTTGCGTCGGCTACCCGCCAGCGACCCGCTTCGCCTGGCCGAGGGGGCAGTACTGCAATTGCAGAAAAGc > 5:116826/1‑149 (MQ=255) ggAGGCAGCGCTGGCCAAAGGGCCTGCTGCCGGGCGTGGGCAGCTGCTGGATGCTGCCAGTGAGCAGTTCATTGCGGCCTTGCGTCGGCTACCCGCCAGCGACCCGCTTCGCCTGGCCGAGGGGGCAGTTCTGCAATTGCAGAAAAGCt > 7:72935/1‑149 (MQ=255) gCAGGGCTGGCCAATGGGCCTGCTGCCGGGCGTGGGCAGCTGCTTGATGCTGCGAGTGAGCAGTTCATTGCGGCCTTGCGTCGGCTACCCGCCAGCGACCCGCTTCGCCTGGCCGAGGGGGCAGTACTGCAATTGCAGAAAAGCTggg < 8:234479/148‑1 (MQ=255) cAAAGGGCCTGCTGCCGGGCGTGGGCAGCTGCTGGATGCTGCCAGTGAGCAGTTCATTGCGGCCTTGCGTCGGCTACCCGCCAGCGACCCGCTTCGCCTGGCCGAGGGGGCAGTACTGCAATTGCAGAAAAGCTGGGGCAAATGGTGcc > 8:71998/1‑149 (MQ=255) ccGGGCGTGGGCAGCTGCTGGATGCTGCCAGTGAGCAGTTCATTGCGGCCTTGCGTCGGCTACCCGCCAGCGACCCGCTTCGCCTGGCCGAGGGGGCAGTACTGCAATTGCAGAAAAGCTGGGGCAAATGGTGCCGCTGGCAggagga > 2:127804/1‑148 (MQ=255) ccGGGCGTGGGCAGCTGCTGGATGCTGCCAGTGAGCAGTTCATTGCGGCCTTGCGTCGGCTACCCGCCAGCGACCCGCTTCGCCTGGCCGAGGGGGCAGTACTGCAATTGCAGAAAAGCTGGGGCAAATGGTGCCGCTGGCAggagga < 5:95341/148‑1 (MQ=255) gggCGTGGGCAGCTGCTGGATGCTGCCAGTGAGCAGTTCATTGCGGCCTTGCGTCGGCTACCCGCCAGCGACCCGCTTCGCCTGGCCGAGGGGGCAGTACTGCAATTGCAGAAAAGCTGGGGCAAATGGTGCCGCTGGCAGGAGGacac > 3:60944/1‑149 (MQ=255) ggCAGCTGCTGGATGCTGCCAGTGAGCAGTTCATTGCGGCCTTGCGTCGGCTACCCGCCAGCGACCCGCTTCGCCTGGCCGAGGGGGCAGTACTGCAATTGCAGAAAAGCTGGGGCAAATGGTGCCGCTGGCAGGAGGACACCCATggg < 1:127804/149‑1 (MQ=255) | CTTCACGGGCTGGATATCGGCGACGAACTGGTGCACCTGCGCATGTGCCTGGCGGTCGCTCAGGCCCCTTTGGGGCGGGCCGAGCGCGAGTACCTGCAACACGTGGAGGCAGCGCTGGCCAAAGGGCCTGCTGCCGGGCGTGGGCAGCTGCTGGATGCTGCCAGTGAGCAGTTCATTGCGGCCTTGCGTCGGCTACCCGCCAGCGACCCGCTTCGCCTGGCCGAGGGGGCAGTACTGCAATTGCAGAAAAGCTGGGGCAAATGGTGCCGCTGGCAGGAGGACACCCATGGG > NC_002947/1318272‑1318562 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |