Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,346,864 A→C 9.1% L10R (CTG→CGG)  udh ← uronate dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,346,8640AC 9.1% 130.3 / 5.0 44L10R (CTG→CGG) udhuronate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (19/21);  new base C (2/2);  total (21/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

GGCCAGGTCACAGGTAATGACTTCTTCATGCGGGCCCGCGGCCGGGGCCATGGGGCTGATGTCAGACAGGCGCAGGACCTCGGCGTAGCCTTTCAGGCGTTCGCGAAGGACCTTGCCCAGGCCGCCTGCGGCTCCGGTGAGCAGCAGGCGATTGAAGGGGGTAGTGGTCATGGCCGGCTCTTGTTGTTAGTTGTTGTAGGTTGTCGTATGACTTGGGTGATTATTGGCGGTGGTTCTGTGTGTTGTCAATGAGGCTTGGGCGGCTTTTGCCGGCCTTTCGCGGGTAAGAAA  >  NC_002947/1346722‑1347012
                                                                                                                                              |                                                                                                                                                    
ggCCAGGTCACAGGTAATGACTTCTTCATGCGGGCCCGCGGCCGGGGCCATGGGGCTGATGTCAGACAGGCGCAGGACCTCGGCGTAGCCTTTCAGGCGTTCGCGAAGGACCTTGCCCAGGCCGCCTGCGGCTCCGGTGAGCAGCAGGc                                                                                                                                                <  6:216721/149‑1 (MQ=255)
ggCCAGGTCACAGGTAATGACTTCTTCATGCGGGCCCGCGGCCGGGGCCATGGGGCTGATGTCAGACAGGCGCAGGACCTCGGCGTAGCCTTTCAGGCGTTCGCGAAGGACCTTGCCAAGGCCGCCTGCGGCTCCGGTGAGCAGCAGGc                                                                                                                                                >  8:245656/1‑149 (MQ=255)
           aGGTAATGACTTCTTCATGCGGGCCCGCGGCCGGGGCCATGGGGCTGATGTCAGACAGGCGCAGGACCTCGGCGTAGCCTTTCAGGCGTTCGCGAAGGACCTTGCCCAGGCCGCCTGCTGCTCACCGGAGCCGCAGGCggcctgg                                                                                                                                         >  6:212665/1‑139 (MQ=255)
           aGGTAATGACTTCTTCATGCGGGCCCGCGGCCGGGGCCATGGGGCTGATGTCAGACAGGCGCAGGACCTCGGCGTAGCCTTTCAGGCGTTCGCGAAGGACCTTGCCCAGGCCGCCTGCTGCTCACCGGAGCCGCAGGCggcctgg                                                                                                                                         <  5:212665/145‑7 (MQ=255)
             gTAATGACTTCTTCATGCGGGCCCGCGGCCGGGGCCATGGGGCTGATGTCAGACAGGCGCAGGACCTCGGCGTAGCCTTTCAGGCGTTCGCGAAGGACCTTGCCCAGGCCGCCTGCGGCTCCGGTGAGCAGCAGGCGATTGTAGGGGGt                                                                                                                                   <  1:136784/149‑1 (MQ=255)
               aaTGACTTCTTCATGCGGGCCCGCGGCCGGGGCCATGGGGCTGATGTCAGACAGGCGCAGGACCTCGGCGTAGCCTTTCAGGCGTTCGCGAAGGACCTTGCCCAGGCCGCCTGCGGCTCCGGTGAGCAGCAGGCGATTGAAGGGGGTAg                                                                                                                                 >  2:17823/1‑149 (MQ=255)
                           aTGCGGGCCCGCGGCCGGGGCCATGGGGCTGATGTCAGACAGGCGCAGGACCTCGGCGTAGCCTTTCAGGCGTTCGCGAAGGACCTTGCCCAGGCCGCCTGCGGCTCCGGTGAGCAGCAGGCGATTGAAGGGGGTAGTGGTCATGGCCg                                                                                                                     <  1:261323/149‑1 (MQ=255)
                           aTGCGGGCCCGCGGCCGGGGCCATGGGGCTGATGTCAGACAGGCGCAGGACCTCGGCGTAGCCTTTCAGGCGTTCGCGAAGGACCTTGCCCAGGCCGCCTGCGGCTCCGGTGAGCAGCAGGCGATTGAAGGGGGTAGTGGTCATGGCCg                                                                                                                     >  4:200109/1‑149 (MQ=255)
                            tGCGGGCCCGCGGCCGGGGCCATGGGGCTGATGTCAGACAGGCGCAGGACCTCGGCGTAGCCTTTCAGGCGTTCGCGAAGGACCTTGCCCAGGCCGCCTGCGGCTCCGGTGAGCAGCAGGCGATTGAAGGGGGTAGTGGTCATGGCCgg                                                                                                                    <  2:24688/149‑1 (MQ=255)
                                 gCCCGCGGCCGGGGCCATGGTGCTGATGTCAGACAGGCGCAGGACCTCGGCGTAGCCTTTCAGGCGTTCGCGAAGGACCTTGCCCAGGCCGCCTGCGGCTCCGGTGAGCAGCAGGCGATTGAAGGGGGTAGTGGTCATGGCCGGCTCtt                                                                                                               <  8:23725/149‑1 (MQ=255)
                                    cgcgGCCGGGGCCATGGGGCTGATGTCAGACAGGCGCAGGACCTCGGCGTAGCCTTTCAGGCGTTCGCGAAGGACCTTGCCCAGGCCGCCTGCGGCTCCGGTGAGCAGCAGGCGATTGAAGGGGGTAGTGGTCATGGCCGGCTCttgtt                                                                                                            <  4:64804/149‑1 (MQ=255)
                                     gcgGCCGGGGCCATGGGGCTGATGTCAGACAGGCGCAGGACCTCGGCGTAGCCTTTCAGGCGTTCGCGAAGGACCTTGCCCAGGCCGCCTGCGGCTCCGGTGAGCAGCAGGCGATTGAAGGGGGTAGTGGTCATGGCCGGCTCttgttg                                                                                                           >  8:61043/1‑149 (MQ=255)
                                     gcgGCCGGGGCCATGGGGCTGATGTCAGACAGGCGCAGGACCTCGGCGTAGCCTTTCAGGCGTTCGCGAAGGACCTTGCCCAGGCCGCCTGCGGCTCCGGTGAGCAGCAGGCGATTGAAGGGGGTAGTGGTCATGGCCGGCTCttgttg                                                                                                           >  8:53359/1‑149 (MQ=255)
                                        gCCGGGGCCATGGGGCTGATGTCAGACAGGCGCAGGACCTCGGCGTAGCCTTTCAGGCGTTCGCGAAGGACCTTGCCCAGGCCGCCTGCGGCTCCGGTGAGCAGCAGGCGATTGAAGGGGGTAGTGGTCATGGCCGGCTCTTGTTGTTa                                                                                                        <  1:17823/149‑1 (MQ=255)
                                            gggCCATGGGGCTGATGTCAGACAGGCGCAGGACCTCGGCGTAGCCTTTCAGGCGTTCGCGAAGGACCTTGCCCAGGCCGCCTGCGGCTCCGGTGAGCAGCAGGCGATTGAAGGGGGTAGTGGTCATGGCCGGCTCTTGTTGTTAgttg                                                                                                    >  1:3194/1‑149 (MQ=255)
                                                          aTGTCAGACAGGCGCAGGACCTCGGCGTAGCCTTTCAGGCGTTCGCGAAGGACCTTGCCCAGGCCGCCTGCGGCTCCGGTGAGCAGCAGGCGATTGAAGGGGGTAGTGGTCATGGCCGGCTCTTGTTGTTAGTTGTTGTAGGTTGTc                                                                                        <  3:200109/147‑1 (MQ=255)
                                                                 aCAGGCGCAGGACCTCGGCGTAGCCTTTCAGGCGTTCGCGAAGGACCTTGCCCAGGCCGCCTGCGGCTCCGGTGAGCAGCAGGCGATTGAAGGGGGTAGTGGTCATGGCCGGCTCTTGTTGTTAGTTGTTGTAGGTTg                                                                                          <  3:130281/138‑1 (MQ=255)
                                                                 aCAGGCGCAGGACCTCGGCGTAGCCTTTCAGGCGTTCGCGAAGGACCTTGCCCAGGCCGCCTGCGGCTCCGGTGAGCAGCAGGCGATTGAAGGGGGTAGTGGTCATGGCCGGCTCTTGTTGTTAGTTGTTGTAGGTTg                                                                                          >  4:130281/1‑138 (MQ=255)
                                                                 aCAGGCGCAGGACCTCGGCGTAGCCTTTCAGGCGTTCGCGAAGGACCTTGCCCAGGCCGCCTGCGGCTCCGGTGAGCAGCAGGCGATTGAAGGGGGTAGTGGTCATGGCCGGCTCTTGTTGTTAGTTGTTGTAGGTTGTCGTATGACtt                                                                               >  4:64440/1‑149 (MQ=255)
                                                                 aCAGGCGCAGGACCTCGGCGTAGCCTTTCAGGCGTTCGCGAAGGACCTTGCCCAGGCCGCCTGCGGCTCCGGTGAGCAGCAGGCGATTGAAGGGGGTAGTGGTCATGGCCGGCTCTTGTTGTTAGTTGTTGTAGGTTGTCGTATGACtt                                                                               <  5:213934/149‑1 (MQ=255)
                                                                     gcgcAGGACCTCGGCGTAGCCTTTCAGGCGTTCGCGAAGGACCTTGCCCAGGCCGCCTGCGGCTCCGGTGAGCAGCAGGCGATTGAAGGGGGTAGTGGTCATGGCCGGCTCTTGTTGTTAGTTGTTGTAGGTTGTCGTATGACTTg                                                                              <  3:25104/146‑1 (MQ=255)
                                                                     gcgcAGGACCTCGGCGTAGCCTTTCAGGCGTTCGCGAAGGACCTTGCCCAGGCCGCCTGCGGCTCCGGTGAGCAGCAGGCGATTGAAGGGGGTAGTGGTCATGGCCGGCTCTTGTTGTTAGTTGTTGTAGGTTGTCGTATGACTTg                                                                              >  4:25104/1‑146 (MQ=255)
                                                                          ggACCTCGGCGTAGCCTTTCAGGCGTTCGCGAAGGACCTTGCCCAGGCCGCCTGCGGCTCCGGTGAGCAGCAGGCGATTGAAGGGGGTAGTGGTCATGGCCGGCTCTTGTTGTTAGTTGTTGTAGGTTGTCGTATGACTTGGGTGatta                                                                      >  4:14488/1‑149 (MQ=255)
                                                                             ccTCGGCGTAGCCTTTCAGGCGTTCGCGAAGGACCTTGCCCAGGCCGCCTGCGGCTCCGGTGAGCAGCAGGCGATTGAAGGGGGTAGTGGTCATGGCCGGCTCTTGTTGTTAGTTGTTGTAGGTTGTCGTATGACTTGGGTGATTATTg                                                                   >  7:177462/1‑149 (MQ=255)
                                                                              cTCGGCGTAGCCTTTCAGGCGTTCGCGAAGGACCTTGCCCAGGCCGCCTGCGGCTCCGGTGAGCAGCAGGCGATTGAAGGGGGTAGTGGTCATGGCCGGCTCTTGTTGTTAGTTGTTGTAGGTTGTCGTATGACTTGGGTGATTATTgg                                                                  <  7:53359/149‑1 (MQ=255)
                                                                               tCGGCGTAGCCTTTCAGGCGTTCGCGAAGGACCTTGCCCAGGCCGCCTGCGGCTCCGGTGAGCAGCAGGCGATTGAAGGGGGTAGTGGTCATGGCCGGCTCTTGTTGTTAGTTGTTGTAGGTTGACGTATGACTTGGGTg                                                                          >  4:147948/1‑140 (MQ=255)
                                                                               tCGGCGTAGCCTTTCAGGCGTTCGCGAAGGACCTTGCCCAGGCCGCCTGCGGCTCCGGTGAGCAGCAGGCGATTGAAGGGGGTAGTGGTCATGGCCGGCTCTTGTTGTTAGTTGTTGTAGGTTGACGTATGACTTGGGTg                                                                          <  3:147948/140‑1 (MQ=255)
                                                                                 ggCGTAGCCTTTCAGGCGTTCGCGAAGGACCTTGCCCAGGCCGCCTGCGGCTCCGGTGAGCAGCAGGCGATTGAAGGGGGTAGTGGTCATGGCCGGCTCTTGTTGTTAGTTGTTGTAGGTTGTCGTATGACTTGGGTGATTATTGGc                                                                 <  8:120589/147‑1 (MQ=255)
                                                                                        ccTTTCAGGCGTTCGCGAAGGACCTTGCCCAGGCCGCCTGCGGCTCCGGTGAGCAGCAGGCGATTGAAGGGGGTAGTGGTCATGGCCGGCTCTTGTTGTTAGTTGTTGTAGGTTGTCGTATGACTTGGGTGATTATTGGCGGTGGTTCt                                                        >  6:62494/1‑149 (MQ=255)
                                                                                            tCAGGCGTTCGCGAAGGACCTTGCCCAGGCCGCCTGCGGCTCCGGTGAGCAGCAGGCGATTGAAGGGGGTAGTGGTCATGGCCGGCTCTTGTTGTTAGTTGTTGTAGGTTGTCGTATGACTTGGGTGATTATTGGCGGTGGTTCtgt                                                      <  8:177462/147‑1 (MQ=255)
                                                                                                gCGTTCGCGAAGGACCTTGCCCAGGCCGCCTGCGGCTCCGGTGAGCAGCAGGCGATTGAAGGGGGTAGTGGTCATGGCCGGCTCTTGTTGTTAGTTGTTGTAGGTTGTCGTATGACTTGGGTGATTATTGGCGGTGGTTCTGTGtgttg                                                <  3:14488/149‑1 (MQ=255)
                                                                                                       cgAAGGACCTTGCCCAGGCCGCCTGCGGCTCCGGTGAGCAGCAGGCGATTGAAGGGGGTAGTGGTCATGGCCGGCTCTTGTTGTTAGTTGTTGTAGGTTGTCGTATGACTTGGGTGATTATTGGCGGTGGTTCTGTGTGTTGTCAATGa                                         <  2:3194/149‑1 (MQ=255)
                                                                                                        gAAGGACCTTGCCCAGGCCGCCTGCGGCTCCGGTGAGCAGCAGGCGATTGAAGGGGGTAGTGGTCATGGCCGGCTCTTGTTGTTAGTTGTTGTAGGTTGTCGTATGACTTGGGTGATTATTGGCGGTGGTTCTGTGTGTTGTCAATGAg                                        >  5:259795/1‑149 (MQ=255)
                                                                                                        gAAGGACCTTGCCCAGGCCGCCTGCGGCTCCGGTGAGCAGCAGGCGATTGAAGGGGGTAGTGGTCATGGCCGGCTCTTGTTGTTAGTTGTTGTAGGTTGTCGTATGACTTGGGTGATTATTGGCGGTGGTTCTGTGTGTTGTCAATGAg                                        >  1:199655/1‑149 (MQ=255)
                                                                                                            gACCTTGCCCAGGCCGCCTGCGGCTCCGGTGAGCAGCAGGCGATTGAAGGGGGTAGTGGTCATGGCCGGCTCTTGTTGTTAGTTGTTGTAGGTTGTCGTATGACTTGGGTGATTATTGGCGGTGGTTCTGTGTGTTGTCAATGAGGCtt                                    >  7:3612/1‑149 (MQ=255)
                                                                                                                    ccAGGCCGCCTGCGGCTCCGGTGAGCAGCAGGCGATTGAAGGGGGTAGTGGTCATGGCCGGCTCTTGTTGTTAGTTGTTGTAGGTTGTCGTATGACTTGGGTGATTATTGGCGGTGGTTCTGTGTGTTGTCAATGAGGCTTGGGCGGCt                            <  6:259795/149‑1 (MQ=255)
                                                                                                                      aGGCCGCCTGCGGCTCCGGTGAGCAGCAGGCGATTGAAGGGGGTAGTGGTCATGGCCGGCTCTTGTTGTTAGTTGTTGTAGGTTGTCGTATGACTTGGGTGATTATTGGCGGTGGTTCTGTGTGTTGTCAATGAGGCTTGGGCGGCttt                          >  7:187427/1‑149 (MQ=255)
                                                                                                                      aGGCCGCCTGCGGCTCCGGTGAGCAGCAGGCGATTGAAGGGGGTAGTGGTCATGGCCGGCTCTTGTTGTTAGTTGTTGTAGGTTGTCGTATGACTTGGGTGATTATTGGCGGTGGTTCTGTGTGTTGTCAATGAGGCTTGGGCGGCttt                          >  3:182906/1‑149 (MQ=255)
                                                                                                                       ggCCGCCTGCGGCTCCGGTGAGCAGCAGGCGATTGAAGGTGGTAGTGGTCATGGCCTGCTCTTGTTGTTAGTTGTTGTAGGTTGTCGTATGACTTGGGTGATTATTGGCGGTGGTTCTGTGTGTTGTCAATGAGGCTTGGGCGGCtttt                         <  7:245656/149‑1 (MQ=255)
                                                                                                                       ggCCGCCTGCGGCTCCGGTGAGCAGCAGGCGATTGAAGGGGGTAGTGGTCATGGCCGGCTCTTGTTGTTAGTTGTTGTAGGTTGTCGTATGACTTGGGTGATTATTGGCGGTGGTTCTGTGTGTTGTCAATGAGGCTTGGGCGGCtttt                         <  5:62494/149‑1 (MQ=255)
                                                                                                                         tcgccTGCTGCTCACCGGAGCCGCAGGCGATTGAAGGGGGTAGTGGTCATGGCCGGCTCTTGTTGTTAGTTGTTGTAGGTTGTCGTATGACTTGGGTGATTATTGGCGGTGGTTCTGTGTGTTGTCAATGAGGCTTGGGCGGCTTTTGc                       >  4:215758/2‑149 (MQ=255)
                                                                                                                                      cGGTGATCAGCAGGCGATTGAAGGGGGTAGTGGTCATGGCCGGCTCTTGTTGTTAGTTGTTGTAGGTTGTCGTATGACTTGGGAGATTATTGGCGGTGGTTCTGTGTGTTGTCAATGAGGCTTGGGCGGCTTTTGCCGGCCTTTcgcg           <  5:42564/148‑1 (MQ=255)
                                                                                                                                      accggAGCCGCAGGCGATTGAAGGGGGTAGTGGTCATGGCCGGCTCTTGTTGTTAGTTGTTGTAGGTTGTCGTATGACTTGGGTGATTATTGGCGGTGGTTCTGTGTGTTGTCAATGAGGCTTGGGCGGCTTTTGCCGGCCTTTcgc            <  3:215758/143‑1 (MQ=255)
                                                                                                                                       ggTGAGCAGCAGGCGATTGAAGGGGGTAGTGGTCATGGCCGGCTCTTGTTGTTAGTTGTTGTAGGTTGTCGTATGACTTGGGTGATTATTGGCGGTGGTTCTGTGTGTTGTCAATGAGGCTTGGGCGGCTTTTGCCGGCCTTTCGCggg         >  7:118147/1‑149 (MQ=255)
                                                                                                                                              agcagGCGATTGAAGGGGGTAGTGGTCATGGCCGGCTCTTGTTGTTAGTTGTTGTAGGTTGTCGTATGACTTGGGTGATTATTGGCGGTGGTTCTGTGTGTTGTCAATGAGGCTTGGGCGGCTTTTGCCGGCCTTTCGCGGGTAAGaaa  <  8:118147/149‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                                    
GGCCAGGTCACAGGTAATGACTTCTTCATGCGGGCCCGCGGCCGGGGCCATGGGGCTGATGTCAGACAGGCGCAGGACCTCGGCGTAGCCTTTCAGGCGTTCGCGAAGGACCTTGCCCAGGCCGCCTGCGGCTCCGGTGAGCAGCAGGCGATTGAAGGGGGTAGTGGTCATGGCCGGCTCTTGTTGTTAGTTGTTGTAGGTTGTCGTATGACTTGGGTGATTATTGGCGGTGGTTCTGTGTGTTGTCAATGAGGCTTGGGCGGCTTTTGCCGGCCTTTCGCGGGTAAGAAA  >  NC_002947/1346722‑1347012

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑