Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,350,501	T→A	8.3%	K11N (AAA→AAT) ‡	PP_1176 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,350,501	0	T	A	8.3%	175.5 / 4.5	60	K11N (AAA→AAT) ‡	PP_1176	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (28/27);  new base A (3/2);  total (31/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTTTCTGACGAGAACGGGATTCCGCTATGTGAGCGTTCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCAC‑CCGTCTGATACTCGATCACACGCGTCTCAAAGAAGTTCTTCTCCTTCTTCAAGTCCATGA‑TCTCGCTCATCCATGGGAACGGGTTGGTA  >  NC_002947/1350361‑1350645                                                                                                                                             |                                                                                                                                                   gTTTCTGACGAGAACGGGATTCCGCTATGTGAGCGTTCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTgg                                                                                                                                                <  6:33190/145‑1 (MQ=255) gTTTCTGACGAGAACGGGATTCCGCTATGTGAGCGTTCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTgg                                                                                                                                                >  5:33190/1‑145 (MQ=255)       gACGAGAACGGGATTCCGCTATGTGAGCGTTCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCttgtt                                                                                                                                      >  5:104003/1‑149 (MQ=255)       gACGAGAACGGGATTCCGCTATGTGAGCGTTCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCttgtt                                                                                                                                      >  6:253955/1‑149 (MQ=255)       gACGAGAACGGGATTCCGCTATGTGAGCGTTCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGACGTAGGAAACGTCATGTTCTGCTTCTGCCAGGGAATAAAAAGGCTGCCATTTGGCAGCCttgtt                                                                                                                                      >  6:243441/1‑149 (MQ=255)          gagaACGGGATTCCGCTATGTGAGCGTTCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGAt                                                                                                                                   >  3:3681/1‑149 (MQ=255)           agaACGGGATTCCGCTATGTGAGCGTTCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATg                                                                                                                                  <  5:211304/149‑1 (MQ=255)             aaCGGGATTCCGCTATGTGAGCGTTCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTg                                                                                                                                                 <  8:237330/132‑1 (MQ=255)             aaCGGGATTCCGCTATGTGAGCGTTCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTg                                                                                                                                                 >  7:237330/1‑132 (MQ=255)                     tCCGCTATGTGAGCGTTCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCgg                                                                                                                        >  2:191506/1‑149 (MQ=255)                       cGCTATGTGAGCGTTCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGt                                                                                                                       >  4:36832/1‑148 (MQ=255)                         cTATGTGAGCGTTCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCt                                                                                                                                <  2:240309/137‑1 (MQ=255)                         cTATGTGAGCGTTCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCt                                                                                                                                >  1:240309/1‑137 (MQ=255)                         cTATGTGAGCGTTCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGt                                                                                                                       >  5:267378/1‑146 (MQ=255)                         cTATGTGAGCGTTCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGt                                                                                                                       <  6:267378/146‑1 (MQ=255)                           aTGTGAGCGTTCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGtcaat                                                                                                                   >  5:70371/1‑148 (MQ=255)                            tgtgAGCGTTCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGATCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGtcaat                                                                                                                   <  6:171830/147‑1 (MQ=255)                             gtgAGCGTTCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCttgttg                                                                                                                                     <  6:85873/128‑1 (MQ=255)                                  cGTTCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATcc                                                                                                             >  4:15011/1‑147 (MQ=255)                                   gTTCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAg                                                                                                          <  6:104003/149‑1 (MQ=255)                                         aaGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCttgttg                                                                                                                                     <  1:150922/116‑1 (MQ=255)                                         aaGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCttgttg                                                                                                                                     >  2:150922/1‑116 (MQ=255)                                               cGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCa                                                                                              >  1:122068/1‑149 (MQ=255)                                                   aaaCGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCAc‑cc                                                                                          <  3:204071/148‑1 (MQ=255)                                                        ttATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCAC‑CCGTCTGa                                                                                    <  5:253955/149‑1 (MQ=255)                                                             tcgATGTCCAAAAACAGGCAAGGTCGGCATCCGTATGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCAC‑CCGTCTGATACTc                                                                               <  8:204472/148‑1 (MQ=255)                                                             gcgATGTCCAAAAACAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCAC‑CCGTCTGATACt                                                                                <  6:70371/148‑1 (MQ=255)                                                                aTGTCCAAAAACAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCAC‑CCGTCTGATACTCGAt                                                                            <  2:122068/149‑1 (MQ=255)                                                                   tCCAAAAACAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCAC‑CCGTCTGATACTCGATca                                                                          >  2:157497/1‑148 (MQ=255)                                                                     cAAAAACAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCCGCCCGGTCAATCAATCCCAGCTCAACGCCCCAC‑CCGTCTGATACTCGATCacac                                                                       >  1:193312/1‑149 (MQ=255)                                                                         aaCAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCAC‑CCGTCTGATACTCGATCACACGCGt                                                                   >  3:120563/1‑149 (MQ=255)                                                                            aGGCAAGGTCGGCATCCGTAGGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCATCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCAC‑CCGTCTGATACTCGATCACACGCGtctc                                                                <  7:208358/149‑1 (MQ=255)                                                                               cAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCAC‑CCGTCTGATACTCGATCACACGCGTCTCaaa                                                             <  3:36832/149‑1 (MQ=255)                                                                                aaGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCAC‑CCGTCTGATACTCGATCACACGCGTCTCa                                                               >  5:124196/1‑146 (MQ=255)                                                                                 aGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCAC‑CCGTCTGATACTCGATCACACGCGTCTCAaag                                                            >  8:113357/1‑148 (MQ=255)                                                                                            cGTAGGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCAC‑CCGTCTGATACTCGATCACACGCGTCTCAAAGAAGTTCTTCTcc                                                <  3:15011/149‑1 (MQ=255)                                                                                              tAGGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCACGCCGTCTGATACTCGATCACACGCGTCTCAAAGAAGTTCTTCTCct                                               <  1:191506/149‑1 (MQ=255)                                                                                                          tGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCAC‑CCGTCTGATACTCGATCACACGCGTCTCAAAGAAGttc                                                      >  3:70217/1‑129 (MQ=255)                                                                                                          tGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCAC‑CCGTCTGATACTCGATCACACGCGTCTCAAAGAAGttc                                                      <  4:70217/129‑1 (MQ=255)                                                                                                                gcttctgcCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCAC‑CCGTCTGATACTCGATCACACGCGTCTCAAAGAAGTTCTTCTCCTTCTTCAAGTCCATGA‑tctc                           >  5:274921/1‑149 (MQ=255)                                                                                                                     tgcCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGc                                                                                                  <  4:260211/75‑1 (MQ=255)                                                                                                                       ccAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCAC‑CCGTCTGATACTCGATCACACGCGTCTCAAAGAAGTTCTTCTCCTTCTTCAAGTCCATGA‑TCTCGCTc                       <  6:124196/146‑1 (MQ=255)                                                                                                                       ccAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCAC‑CCGTCTGATACTCGATCACACGCGTCTCAAAGAAGTTCTTCTCCTTCTTCAAGTCCATGA‑TCTCGCTCATc                    >  6:79817/1‑149 (MQ=255)                                                                                                                             atcaacaaGGCTGCCAAATGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCAC‑CCGTCTGATACTCGATCACACGCGTCTCAAAGAAGTTCTTCTCCTTCTTCAAGTCCATGA‑TCTCGCTCATCCATgg               >  5:181428/7‑148 (MQ=255)                                                                                                                             aaTAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCAC‑CCGTCTGATACTCGATCACACGCGTCTCAAAGAAGTTCTTCTCCTTCTTCAAGTCCATGA‑TCTCGCTCATCCAt                 <  3:125387/146‑1 (MQ=255)                                                                                                                             aaTAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCAC‑CCGTCTGATACTCGATCACACGCGTCTCAAAGAAGTTCTTCTCCTTCTTCAAGTCCATGA‑TCTCGCTCATCCAt                 >  4:125387/1‑146 (MQ=255)                                                                                                                             aaTAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCAC‑CCGTCTGATACTCGATCACACGCGTCTCAAAGAAGTTCTTCTCCTTCTTCAAGTCCATGA‑TCTCGCTCATCCATgg               >  5:28034/1‑148 (MQ=255)                                                                                                                              tcaacaaGGCTGCCAAATGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCAC‑CCGTCTGATACTCGATCACACGCGTCTCAAAGAAGTTCTTCTCCTTCTTCAAGTCCATGA‑TCTCGCTCATCCATggg              >  6:151294/6‑148 (MQ=255)                                                                                                                                    aGGCTGCCAAATGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCAC‑CCGTCTGATACTCGATCACACGCGTCTCAAAGAAGTTCTTCTCCTTCTTCAAGTCCATGA‑TCTCGCTCATCCATGGGAACGGGt       <  6:181428/149‑1 (MQ=255)                                                                                                                                    aGGCTGCCAAATGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCAC‑CCGTCTGATACTCGATCACACGCGTCTCAAAGAAGTTCTTCTCCTTCTTCAAGTCCATGA‑TCTCGCTCATCCATGGGAACGGGt       >  8:163277/1‑149 (MQ=255)                                                                                                                                      gCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCAC‑CCGTCTGATACTCGATCACACGCGTCTCAAAGAAGTTCTTCTCCTTCTTCAAGTCCATCA‑TCTCGCTCATCAATGGGAACTGGttt     >  4:32923/1‑148 (MQ=255)                                                                                                                                       cTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCAC‑CCGTCTGATACTCGATCACACGCGTCTCAAAGAAGTTCTTCTCCTTCTTCAAGTCCATGA‑TCTCGCTCATCCATGGGAACGGGTTgg    <  6:274921/149‑1 (MQ=255)                                                                                                                                       cTGCCAAATGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCAC‑CCGTCTGATACTCGATCACACGCGTCTCAAAGAAGTTCTTCTCCTTCTTCAAGTCCATGA‑TCTCGCTCATCCATGGGAACGGGTTgg    <  5:151294/149‑1 (MQ=255)                                                                                                                                        tGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCAC‑CCGTCTGATACTCGATCACACGCGTCTCAaagaa                                                          <  2:27099/95‑1 (MQ=255)                                                                                                                                        tGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCAC‑CCGTCTGATACTCGATCACACGCGTCTCAaagaa                                                          >  1:27099/1‑95 (MQ=255)                                                                                                                                        tGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCAC‑CCGTCTGATACTCGATCACACGCGTCTCAAAGAAGTTCTTCTCCTTCTTCAAGTCCATGA‑TCTCGCTCATCCATGGGAACGGGTTg     <  4:27187/147‑1 (MQ=255)                                                                                                                                        tGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCAC‑CCGTCTGATACTCGATCACACGCGTCTCAAAGAAGTTCTTCTCCTTCTTCAAGTCCATGA‑TCTCGCTCATCCATGGGAACGGGTTg     >  3:27187/1‑147 (MQ=255)                                                                                                                                          ccATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCAC‑CCGTCTGATACTCGATCACACGCGTCTCAAAGAAGTTCTTCTCCTTCTTCAAGTCCATGACTCTCGCTCATCCATGGGAACGGGTTGGTa  <  1:157497/149‑1 (MQ=255)                                                                                                                                          ccATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCAC‑CCGTCTGATACTCGATCACACGCGTCTCAAAGAAGTTCTTCTCCTTCTTCAAGTCCATGA‑TCTCGCTCATCCATGGGAACg          <  7:188191/140‑1 (MQ=255)                                                                                                                                          ccATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCAC‑CCGTCTGATACTCGATCACACGCGTCTCAAAGAAGTTCTTCTCCTTCTTCAAGTCCATGA‑TCTCGCTCATCCATGGGAACg          >  8:188191/1‑140 (MQ=255)                                                                                                                                             |                                                                                                                                                   GTTTCTGACGAGAACGGGATTCCGCTATGTGAGCGTTCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCAC‑CCGTCTGATACTCGATCACACGCGTCTCAAAGAAGTTCTTCTCCTTCTTCAAGTCCATGA‑TCTCGCTCATCCATGGGAACGGGTTGGTA  >  NC_002947/1350361‑1350645

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑