Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 170,419 A→G 8.1% Q274R (CAG→CGG)  PP_0159 → family III CoA‑transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947170,4190AG 8.1% 138.8 / 2.7 49Q274R (CAG→CGG) PP_0159family III CoA‑transferase
Reads supporting (aligned to +/- strand):  ref base A (20/25);  new base G (2/2);  total (22/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.45e-01

CCTGACCACGGGCACCCCTCCCCGTCGGCTGGGTAATGCGCATCCTAATATAGTCCCTTACCAGGACTTTCCGACGGCAGATGGCGATTTCATTCTTACCGTCGGTAATGACAGCCAGTTCCGCAAGTTCGCTGAGGTGGCCGGGCAGCCGCAATGGGCGGAGGACCCTCGGTTCGTTACCAATAAGCTGCGGGTCGCCAACCGGGCTGAGCTGATACCTTTGATTCGCCAGGCTACGGTGTTCAAGACCACGGCTGAGTGGGTGGCCCAGTTGGAGGCTGCAGGAGTGCC  >  NC_002947/170273‑170563
                                                                                                                                                  |                                                                                                                                                
ccTGACCACGGGCACCCCTCCCCGTCGGCTGGGTAATGCGCATCCTAATATAGTCCCTTACCAGGACTTTCCGACGGCAGATGGCGATTTCATTCTTACCGTCGGTAATGACAGCCAGTTCCGCAAGTTCGCTGAGGTGGCCGGGCAGc                                                                                                                                                >  5:195161/1‑149 (MQ=255)
    aCCACGGGCACCCCTCCCCGTCGGCTGGGTAATGCGCATCCTAATATAGTCCCTTACCAGGACTTTCCGACGGCAGATGGCGATTTCATTCTTACCGTCGGTAATGACAGCCAGTTCCGCAAGTTCGCTGAGGTGGCCGGGCAGCCg                                                                                                                                              <  3:198430/147‑1 (MQ=255)
             aCCCCTCCCCGTCGGCTGGGTAATGCGCATCCTAATATAGTCCCTTACCAGGACTTTCCGACGGCAGATGGCGATTTCATTCTTACCGTCGGTAATGACAGCCAGTTCCGCAAGTTCGCTGAGGTGGCCGGGCAGCCGCAATGGGCgga                                                                                                                                   <  1:246767/149‑1 (MQ=255)
                    cccGTCGGCTGGGTAATGCGCATCCTAATATAGTCCCTTACCAGGACTTTCCGACGGCAGATGGCGATTTCATTCTTACCGTCGGTAATGACAGCCAGTTCCGCAAGTTCGCTGAGGTGGCCGGGCAGCCGCAATGGGCGGAGGACCCt                                                                                                                            >  7:91334/1‑149 (MQ=255)
                     ccGTCGGCTGGGTAATGCGCATCCTAATATAGTCCCTTACCAGGACTTTCCGACGGCAGATGGCGATTTCATTCTTACCGTCGGTAATGACAGCCAGTTCCGCAAGTTCGCTGAGGTGGCCGGGCGGCCGCAATGGGCGGAGGACCCTc                                                                                                                           >  1:18976/1‑149 (MQ=255)
                     ccGTCGGCTGGGTAATGCGCATCCTAATATAGTCCCTTACCAGGACTTTCCGACGGCAGATGGCGATTTCATTCTTACCGTCGGTAATGACAGCCAGTTCCGCAAGTTCGCTGAGGTGGCCGGGCGGCCGCAATGGGCGGAGGACCCTc                                                                                                                           >  1:23195/1‑149 (MQ=255)
                     ccGTCGGCTGGGTAATGCGCATCCTAATATAGTCCCTTACCAGGACTTTCCGACGGCAGATGGCGATTTCATTCTTACCGTCGGTAATGACAGCCAGTTCCGCAAGTTCGCTGAGGTGGCCGGGCAGCCGCAATGGGCGGAGGACCCTc                                                                                                                           >  1:240621/1‑149 (MQ=255)
                          ggCTGGGTAATGCGCATCCTAATATAGTCCCTTACCAGGACTTTCCGACGGCAGATGGCGATTTCATTCTTACCGTCGGTAATGACAGCCAGTTCCGCAAGTTCGCTGAGGTGGCCGGGCGGCCGCAATGGGCGGAGGACCCTCGGTTc                                                                                                                      <  2:23195/149‑1 (MQ=255)
                          ggCTGGGTAATGCGCATCCTAATATAGTCCCTTACCAGGACTTTCCGACGGCAGATGGCGATTTCATTCTTACCGTCGGTAATGACAGCCAGTTCCGCAAGTTCGCTGAGGTGGCCGGGCGGCCGCAATGGGCGGAGGACCCTCGGTTc                                                                                                                      <  2:18976/149‑1 (MQ=255)
                           gCTGGGTAATGCGCATCCTAATATAGTCCCTTACCAGGACTTTCCGACGGCAGATGGCGATTTCATTCTTACCGTCGGTAATGACAGCCAGTTCCGCAAGTTCGCTGAGGTGGCCGGGCAGCCGCAATGGGCGGAGGACCCTCGGTTCg                                                                                                                     >  4:86434/1‑149 (MQ=255)
                               ggTAATGCGCATCCTAATATAGTCCCTTACCAGGACTTTCCGACGGCAGATGGCGATTTCATTCTTACCGTCGGTAATGACAGCCAGTTCCGCAAGTTCGCTGAGGTGGCCGGGCAGCCGCAATGGGCGGAGGACCCTCGGTTCGTTAc                                                                                                                 <  4:213567/149‑1 (MQ=255)
                                            cTAATATAGTCCCTTACCAGGACTTTCCGACGGCAGATGGCGATTTCATTCTTACCGTCGGTAATGACAGCCAGTTCCGCAAGTTCGCTGAGGTGGCCGGGCAGCCGCAATGGGCGGAGGACCCTCGGTTCGTTACCAATAAGCTGCgg                                                                                                    <  6:195161/149‑1 (MQ=255)
                                            cTAATATAGTCCCTTACCAGGACTTTCCGACGGCAGATGGCGATTTCATTCTTACCGTCGGTAATGACAGCCAGTTCCGCAAGTTCGCTGAGGTGGCCGGGCAGCCGCAATGGGCGGAGGACCCTCGGTTCGTTACCAATAAGCTGCgg                                                                                                    <  7:25282/149‑1 (MQ=255)
                                            cTAATATAGTCCCTTACCAGGACTTTCCGACGGCAGATGGCGATTTCATTCTTACCGTCGGTAATGACAGCCAGTTCCGCAAGTTCGCTGAGGTGGCCGGGCAGCCGCAATGGGCGGAGGACCCTCGGTTCGTTACCAATAAGCTGCg                                                                                                     <  8:91334/148‑1 (MQ=255)
                                                   aGTCCCTTACCAGGACTTTCCGACGGCAGATGGCGATTTCATTCTTACCGTCGGTAATGACAGCCAGTTCCGCAAGTTCGCTGAGGTGGCCGGGCAGCCGCAATGGGCGGAGGACCCTCGGTTCGTTACCAATAAGCTGCGGGTCGcc                                                                                              >  4:60354/1‑148 (MQ=255)
                                                       ccTTACCAGGACTTTCCGACGGCAGATGGCGATTTCATTCTTACCGTCGGTAATGACAGCCAGTTCCGCAAGTTCGCTGAGGTGGCCGGGCAGCCGCAATGGGCGGAGGACCCTCGGTTc                                                                                                                      <  6:122613/120‑1 (MQ=255)
                                                       ccTTACCAGGACTTTCCGACGGCAGATGGCGATTTCATTCTTACCGTCGGTAATGACAGCCAGTTCCGCAAGTTCGCTGAGGTGGCCGGGCAGCCGCAATGGGCGGAGGACCCTCGGTTc                                                                                                                      >  5:122613/1‑120 (MQ=255)
                                                         ttACCAGGACTTTCCGACGGCAGATGGCGATTTCATTCTTACCGTCGGTAATGACAGCCAGTTCCGCAAGTTCGCTGAGGTGGCCGGGCAGCCGCAATGGGCGGAGGACCCTCGGTTCGTTACCAATAAGCTGCGGGTCGCCAACCggg                                                                                       >  7:3058/1‑149 (MQ=255)
                                                            ccAGGACTTTCCGACGGCAGATGGCGATTTCATTCTTACCGTCGGTAATGACAGCCAGTTCCGCAAGTTCGCTGAGGTGGCCGGGCAGCCGCAATGGGCGGAGGACCCTCgg                                                                                                                         <  6:1525/112‑1 (MQ=255)
                                                            ccAGGACTTTCCGACGGCAGATGGCGATTTCATTCTTACCGTCGGTAATGACAGCCAGTTCCGCAAGTTCGCTGAGGTGGCCGGGCAGCCGCAATGGGCGGAGGACCCTCgg                                                                                                                         >  5:1525/1‑112 (MQ=255)
                                                            ccAGGACTTTCCGACGGCAGATGGCGATTTCATTCTTACCGTCGGTAATGACAGCCAGTTCCGCAAGTTCGCTGAGGTGGCCGGGCAGCCGCAATGGGCGGAGGACCCTCGGTTCGTTACCAATAAGCTGCGGGTCGCCAACCGGgct                                                                                     <  2:163454/148‑1 (MQ=255)
                                                             cAGGACTTTCCGACGGCAGATGGCGATTTCATTCTTACCGTCGGTAATGACAGCCAGTTCCGCAAGTTCGCTGAGGTGGCCGGGCAGCCGCAATGGGCGGAGGACCCTCGGTTCGTTACCAATAAGCTGCGGGTCGCCAACCGGgct                                                                                     >  5:277110/1‑147 (MQ=255)
                                                               ggACTTTCCGACGGCAGATGGCGATTTCATTCTTACCGTCGGTAATGACAGCCAGTTCCGCAAGTTCGCTGAGGTGGCCGGGCAGCCGCAATGGGCGGAGGACCCTCGGTTCGTTACCAATAAGCTGCGGGTCGCCAACCGGgctgagc                                                                                 <  3:60354/149‑1 (MQ=255)
                                                               ggACTTTCCGACGGCAGATGGCGATTTCATTCTTACCGTCGGTAATGACAGCCAGTTCCGCAAGTGCGCTGAGGTGGCCGGGCAGCCGCAATGGGCGGAGGACCCTCGGTTCGTTACCAATACGCTGCCGGTCGCCAACCGGgctgagc                                                                                 <  1:163655/149‑1 (MQ=255)
                                                                        gACGGCAGATGGCGATTTCATTCTTACCGTCGGTAATGACAGCCAGTTCCGCAAGTTCGCTGAGGTGGCCGGGCAGCCGCAACGGGCGGAGGACCCTCGGTTCGTTACCAATAAGCTGCGGGTCGCCAACCGGGCTGAGCTGATACCtt                                                                        >  1:210614/1‑149 (MQ=255)
                                                                         aCGGCAGATGGCGATTTCATTCTTACCGTCGGTAATGACAGCCAGTTCCGCAAGTTCGCTGAGGTGGCCGGGCAGCCGCAATGGGCGGAGGACCCTCGGTTCGTTACCAATAAGCTGCgg                                                                                                    >  1:161935/1‑120 (MQ=255)
                                                                         aCGGCAGATGGCGATTTCATTCTTACCGTCGGTAATGACAGCCAGTTCCGCAAGTTCGCTGAGGTGGCCGGGCAGCCGCAATGGGCGGAGGACCCTCGGTTCGTTACCAATAAGCTGCgg                                                                                                    <  2:161935/120‑1 (MQ=255)
                                                                              aGATGGCGATTTCATTCTTACCGTCGGTAATGACAGCCAGTTCCGCAAGTTCGCTGAGGTGGCCGGGCAGCCGCAATGGGCGGAGGACCCTCGGTTCGTTACCAATAAGCTGCGGGTCGCCAACCGGGCTGAGCTGATACCTTTGATTc                                                                  <  6:215162/149‑1 (MQ=255)
                                                                                    cGATTTCATTCTTACCGTCGGTAATGACAGCCAGTTCCGCAAGTTCGCTGAGGTGGCCGGGCAGCCGCAATGGGCGGAGGACCCTCGGTTCGTTACCAATAAGCTGCGGGTCGCCAACCGGGCTGAGCTGATACCTTTGATTCGCCAgg                                                            <  2:33977/149‑1 (MQ=255)
                                                                                     gATTTCATTCTTACCGTCGGTAATGACAGCCAGTTCCGCAAGTTCGCTGAGGTGGCCGGGCAGCCGCAATGGGCGGAGGACCCTCGGTTCGTTACCAATAAGCTGCGGGTCGCCAACCGGGCTGAGCTGATACCTTTGATTCGCCAGGc                                                           >  8:103877/1‑149 (MQ=255)
                                                                                          cATTCTTACCGTCGGTAATGACAGCCAGTTCCGCAAGTTCGCTGAGGTGGCCGGGCAGCCGCAATGGGCGGAGGACCCTCGGTTCGTTACCAATAAGCTGCGGGTCGCCAACCGGGCTGAGCTGATACCTTTGATTCGCCAGGCTACg                                                       <  2:240621/148‑1 (MQ=255)
                                                                                                  ccGTCGGTAATGACAGCCAGTTCCGCAAGTTCGCTGAGGTGGCCGGGCAGCCGCAATGGGCGGAGGACCCTCGGTTCGTTACCAATAAGCTGCGGGTCGCCAACCGGGCTGAGCTGATACCTTTGATTCGCCAGGCTACGGTGTTc                                                 >  8:209625/1‑146 (MQ=255)
                                                                                                   cGTCGGTAATGACAGCCAGTTCCGCAAGTTCGCTGAGGTGGCCGGGCAGCCGCAATGGGCGGAGGACCCTCGGTTCGTTACCAATAAGCTGCGGGTCGCCAACCGGGCTGAGCTGATACCTTTGATTCGCCAGGCTACGGTGTTCAAGa                                             <  3:86434/149‑1 (MQ=255)
                                                                                                     tCGGTAATGACAGCCAGTTCCGCAAGTTCGCTGAGGTGGCCGGGCAGCCGCAATGGGCGGAGGACCCTCGGTTCGTTACCAATAAGCTGCGGGTCGCCAACCGGgctgagctga                                                                              <  4:176098/114‑1 (MQ=255)
                                                                                                     tCGGTAATGACAGCCAGTTCCGCAAGTTCGCTGAGGTGGCCGGGCAGCCGCAATGGGCGGAGGACCCTCGGTTCGTTACCAATAAGCTGCGGGTCGCCAACCGGgctgagctga                                                                              >  3:176098/1‑114 (MQ=255)
                                                                                                        gTAATGACAGCCAGTTCCGCAAGTTCGCTGAGGTGGCCGGGCAGCCGCAATGGGCGGAGGACCCTCGGTTCGTTACCAATAAGCTGCGGGTCGCCAACCGGGCTGAGCTGATACCTTTGATTCGCCAGGCTACGGTGTTc                                                 <  6:265351/140‑1 (MQ=255)
                                                                                                        gTAATGACAGCCAGTTCCGCAAGTTCGCTGAGGTGGCCGGGCAGCCGCAATGGGCGGAGGACACTCGGTTCGTTACCAATAAGCTGCGGGTCGCCAACCGGGCTGAGCTGATACCTTTGATTCGCCAGGCTACGGTGTTc                                                 >  5:265351/1‑140 (MQ=255)
                                                                                                        gTAATGACAGCCAGTTCCGCAAGTGCGCTGAGGTGGCCGGGCAGCCGCAATGGGCGGAGGACCCTCGGTTCGTTACCAATAAGCTGCGGGTCGCCAACCGGGCTGAGCTGATACCTTTGATTCGCCAGGCTACGGTGTTCAAGACCACg                                        >  8:248469/1‑149 (MQ=255)
                                                                                                         tAATGACAGCCAGTTCCGCAAGTTCGCTGAGGTGGCCGGGCAGCCGCAATGGGCGGAGGACCCTCGGTTCGTTACCAATAAGCTGCGGGTCGCCAACCGGGCTGAGCTGATACCTTTGATTCGCCAGGCTACGGTGTTCACGACCACgg                                       >  4:86027/1‑149 (MQ=255)
                                                                                                           aTGACAGCCAGTTCCGCAAGTTCGCTGAGGTGGCCGGGCAGCCGCAATGGGCGGAGGACCCTCGGTTCGTTACCAATAAGCTGCGGGTCGCCAACCGGGCTGAGCTGATACCTTTGATTCGCCAGGCTACGGTGTTCAAGACCACGGCt                                     <  7:209625/149‑1 (MQ=255)
                                                                                                                 gCCAGTTCCGCAAGTTCGCTGAGGTGGCCGGGCAGCCGCAATGGGCGGAGGACCCTCGGTTCGTTACCAATAAGCTGCGGGTCGCCAACCGGGCTGAGCTGATACCTTTGATTCGCCAGGCTACGGTGTTCAAGACCACGGCTGAgtgg                               <  7:103877/149‑1 (MQ=255)
                                                                                                                  ccAGTTCCGCAAGTTCGCTGAGGTGGCCGGGCAGCCGCAATGGGCGGAGGACCCTCGGTTCGTTACCAATAAGCTGCGGGTCGCCAACCGGGCTGAGCTGATACCTTTGATTCGCCAGGCTACGGTGTTCAAGACCACGGCTGAgtggg                              >  1:149642/1‑149 (MQ=255)
                                                                                                                   cAGGTCCGCAAGTTCGCTGAGGTGGCCGGGCAGCCGCAATGGGCGGAGGACCCTCGGTTCGTTACCAAAAAGCTGCGGGTCGCCAACCGGGCTGAGCTGATACCTTTGATTCGCCAGGCTACGGTGTTCAAGACCACGGCTGAgtgggt                             <  8:196498/149‑1 (MQ=255)
                                                                                                                          gCAAGTTCGCTGAGGTGGCCGGGCAGCCGCAATGGGCGGAGGACCCTCGGTTCGTTACCAATAAGCTGCGGGTCGCCAACCGGGCTGAGCTGATACCTTTGATTCGCCAGGCTACGGTGTTCAAGACCACGGCTGAGTGGGTGGCCCAg                      <  6:277110/149‑1 (MQ=255)
                                                                                                                                 cGCTGAGGTGGCCGGGCAGCCGCAATGGGCGGAGGACCCTCGGTTCGTTACCAATAAGCTGCGGGTCGCCAACCGGGCTGAGCTGATACCTTTGATTCGCCAGGCTACGGTGTTCAAGACCACGGCTGAGTGGGTGGCCCAGTTGGAg                <  8:3058/148‑1 (MQ=255)
                                                                                                                                  gCTGAGGTGGCCGGGCAGCCGCAATGGGCGGAGGACCCTCGGTTCGTTACCAATAAGCTGCGGGTCGCCAACCGGGCTGAGCTGATACCTTTGATTCGCCAGGCTACGGTGTTCAAGACCACGGCTGAGTGGGTGGCCCAGTTGGAGGc              >  6:6307/1‑149 (MQ=255)
                                                                                                                                    tGAGGTGGCCGGGCAGCCGCAATGGGCGGAGGACCCTCGGTTCGTTCCCAATAAGCTGCGGGTCGCCACCCGGGCTGAGCTGATACCTGTGATTCGCCAGGCTACGGTGTTCAAGACCACGGCTGAGTGGGTGGCCCAGTTGGAGGCTg            >  7:56219/1‑149 (MQ=255)
                                                                                                                                            ccGGGCAGCCGCAATGGGCGGAGGACCCTCGGTTCGTTACCAATAAGCTGCGGGTCGCCAACCGGGCTGAGCTGATACCTTTGATTCGCCAGGCTACGGTGTTCAAGACCACGGCTGAGTGGGTGGCCCAGTTGGAGGCTGCAGGAGTg    <  5:23400/149‑1 (MQ=255)
                                                                                                                                              gggCAGCCGCAATGGGCGGAGGACCCTCGGTTCGTTACCAATAAGCTGCGGGTCGCCAACCGGGCTGAGCTGATACCTTTGATTCGCCAGGCTACGGTGTTCAAGACCACGGCTGAGTGGGTGGCCCAGTTGGAGGCTGCAGGAGTGcc  <  2:149642/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
CCTGACCACGGGCACCCCTCCCCGTCGGCTGGGTAATGCGCATCCTAATATAGTCCCTTACCAGGACTTTCCGACGGCAGATGGCGATTTCATTCTTACCGTCGGTAATGACAGCCAGTTCCGCAAGTTCGCTGAGGTGGCCGGGCAGCCGCAATGGGCGGAGGACCCTCGGTTCGTTACCAATAAGCTGCGGGTCGCCAACCGGGCTGAGCTGATACCTTTGATTCGCCAGGCTACGGTGTTCAAGACCACGGCTGAGTGGGTGGCCCAGTTGGAGGCTGCAGGAGTGCC  >  NC_002947/170273‑170563

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑