Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,360,967 C→G 7.1% L11V (CTG→GTG) ‡ phoP → two‑component system DNA‑binding transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,360,9670CG 7.1% 173.9 / 4.9 56L11V (CTG→GTG) ‡phoPtwo‑component system DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (26/26);  new base G (2/2);  total (28/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.60e-01

TGCAGTGCCTTGGGTTTCTGCAGGTGCTACACAGGTTTCAGGGACTGTGCGATTACTGTGGGAGCGGGCGTGCCCGCGAAAGGGCCGGTACAGGCAATACACATAAGGAGCTGCACATGAAACTGCTGGTGGTCGAGGACGAAGCCCTGCTTCGCCATCACCTCTACACCCGCCTGGGCGAAAGCGGCCATGTG‑GTCGAGGCGGTCGCTGATGCCGAAGAGGCGCTGTACCAGGCCGAGCAGTACCATTTCGACCTTGCCATCATCGACCTGGGCCTGCCCGGTATCAG  >  NC_002947/1360821‑1361109
                                                                                                                                                  |                                                                                                                                               
tgCAGTGCCTTGGGTTTCTGCAGGTGCTACACAGGTTTCAGGGACTGTGCGATTACTGTGGGAGCGGGCGTGCCCGCGAAAGGGCCGGTACAGGCAATACACATAAGGAGCTGCACATGAAACTGCTGGTGGTCGAGGACGAAGCCCTg                                                                                                                                               >  4:127539/1‑149 (MQ=255)
  cAGTGCCTTGGGTTTCTGCAGGTGCTACACAGGTTTCAGGGACTGTGCGATTACTGTGGGAGCGGGCGTGCCCGCGAAAGGGCCGGTACAGGCAATACACATAAGGAGCTGCACATGAAACTGCTGGTGGTCGAGGACGAAGCCCTGc                                                                                                                                              <  3:127539/148‑1 (MQ=255)
       ccTTGGGTTTCTGCAGGTGCTACACAGGTTTCAGGGACTGTGCGATTACTGTGGGAGCGGGCGTGCCCGCGAAAGGGCCGGTACAGGCAATACACATAAGGAGCTGCACATGAAACTGCTGGTGGTCGAGGTCGAAGCCCTGCTTCGcc                                                                                                                                        <  7:48020/149‑1 (MQ=255)
             gTTTCTGCAGGTGCTACACAGGTTTCAGGGACTGTGCGATTACTGTGGGAGCGGGCGTGCCCGCGAAAGGGCCGGTACAGGCAATACACATAAGGAGCTGCACATGAAACTGCTGGTGGTCGAGGACGAAGCCCTGCTTCGCCATCAcc                                                                                                                                  >  4:193087/1‑149 (MQ=255)
              tttCTGCAGGTGCTACACAGGTTTCAGGGACTGTGCGATTACTGTGGGAGCGGGCGTGCCCGCGAAAGGGCCGGTACAGGCAATACACATAAGGAGCTGCACATGAAACTGCTGGTGGTCGAGGACGAAGCCCTGCTTCg                                                                                                                                          >  7:229489/1‑140 (MQ=255)
              tttCTGCAGGTGCTACACAGGTTTCAGGGACTGTGCGATTACTGTGGGAGCGGGCGTGCCCGCGAAAGGGCCGGTACAGGCAATACACATAAGGAGCTGCACATGAAACTGCTGGTGGTCGAGGACGAAGCCCTGCTTCg                                                                                                                                          <  8:229489/140‑1 (MQ=255)
                  tGCAGGTGCTACACAGGTTTCAGGGACTGTGCGATTACTGTGGGAGCGGGCGTGCCCGCGAAAGGGCCGGTACAGGCAATACACATAAGGAGCTGCACATGAAACTGCTGGTGGTCGAGGACGAAGCCCTGCTTCGCCATCACCTCTa                                                                                                                              >  5:97613/1‑148 (MQ=255)
                          cTACACAGGTTTCAGGGACTGTGCGATTACTGTGGGAGCGGGCGTGCCCGCGAAAGGGCCGGTACAGGCAATACACATAAGGAGCTGCACATGAAACTGCTGGTGGTCGAGGACGAAGCCCTGCTTCGCCATCACCTCTACACCCGCCt                                                                                                                     <  6:164994/149‑1 (MQ=255)
                              acaGGTTTCAGGGACTGTGCGATTACTGTGGGAGCGGGCGTGCCCGCGAAAGGGCCGGTACAGGCAATACACATAAGGAGCTGCACATGAAACTGCTGGTGGTCGAGGGCGAAGCAGGGCTTCgtcc                                                                                                                                       >  3:221640/1‑124 (MQ=38)
                              acaGGTTTCAGGGACTGTGCGATTACTGTGGGAGCGGGCGTGCCCGCGAAAGGGCCGGTACAGGCAATACACATAAGGAGCTGCACATGAAACTGCTGGTGGTCGAGGGCGAAGCAGGGCTTCgtcc                                                                                                                                       <  4:225334/127‑4 (MQ=38)
                              acaGGTTTCAGGGACTGTGCGATTACTGTGGGAGCGGGCGTGCCCGCGAAAGGGCCGGTACAGGCAATACACATAAGGAGCTGCACATGAAACTGCTGGTGGTCGAGGGCGAAGCAGGGCTTCgtcc                                                                                                                                       <  4:221640/127‑4 (MQ=38)
                              acaGGTTTCAGGGACTGTGCGATTACTGTGGGAGCGGGCGTGCCCGCGAAAGGGCCGGTACAGGCAATACACATAAGGAGCTGCACATGAAACTGCTGGTGGTCGAGGGCGAAGCAGGGCTTCgtcc                                                                                                                                       >  3:225334/1‑124 (MQ=38)
                              acaGGTTTCAGGGACTGTGCGATTACTGTGGGAGCGGGCGTGCCCGCGAAAGGGCCGGTACAGGCAATACACATAAGGAGCTGCACATGAAACTGCTGGTGGTCGAGGACGAAGCCCTGCTTCGCCATCACCTCTACACCCGCCTGGGc                                                                                                                 >  2:108573/1‑149 (MQ=255)
                                   tttCAGGGACTGTGCGATTACTGTGGGAGCGGGCGTGCCCGCGAAAGGGCCGGTACAGGCAATACACATAAGGAGCTGCACATGAAACTGCTGGTGGTCGAGGACGAAGCCCTGCTTCGCCATCACCTCTACACCCGCCTGGGCGAAAg                                                                                                            >  8:202608/1‑149 (MQ=255)
                                       aGGGACTGTGCGATTACTGTGGGAGCGGGCGTGCCCGCGAAAGGGCCGGTACAGGCAATACACATAAGGAGCTGCACATGAAACTGCTGGTGGTCGAGGACGAAGCCCTGCTTCGCCATCACCTCTACACCCGCCTGGGCGAAAGCgg                                                                                                         <  1:156302/148‑1 (MQ=255)
                                       aGGGACTGTGCGATTACTGTGGGAGCGGGCGTGCCCGCGAAAGGGCCGGTACAGGCAATACACATAAGGAGCTGCACATGAAACTGCTGGTGGTCGAGGACGAAGCCCTGCTTCGCCATCACCTCTACACCCGCCTGGGCGAAAGCgg                                                                                                         <  7:202608/148‑1 (MQ=255)
                                             tgtgCGATTACTGTGGGAGCGGGCGTGCCCGCGAAAGGGCCGGTACAGGCAATACACATAAGGAGCTGCACATGAAACTGCTGGTGGTCGAGGACGAAGCCCTGCTTCGCCATCACCTCTACACCCGCCTGGGCGAAAGCGGCCAtgtg                                                                                                  >  7:210055/1‑149 (MQ=255)
                                               tgCGATTACTGTGGGAGCGGGCGTGCCCGCGAAAGGGCCGGTACAGGCAATACACATAAGGAGCTGCACATGAAACTGCTGGTGGTCGAGGACGAAGCCCTGCTTCGCAATCACCTCTACACCCGCCTGGGCGAAAGTGGCCATGTG‑Gt                                                                                               >  5:53960/1‑149 (MQ=255)
                                                       cTGTGGGAGCGGGCGTGCCCGCGAAAGGGCCGGTACAGGCAATACACATAAGGAGCTGCACATGAAACTGCTGGTGGTCGAGGACGAAGCCCTGc                                                                                                                                              >  6:38673/1‑95 (MQ=255)
                                                       cTGTGGGAGCGGGCGTGCCCGCGAAAGGGCCGGTACAGGCAATACACATAAGGAGCTGCACATGAAACTGCTGGTGGTCGAGGACGAAGCCCTGc                                                                                                                                              <  5:38673/95‑1 (MQ=255)
                                                       cTGTGGGAGCGGGCGTGCCCGCGAAAGGGCCGGTACAGGCAATACACATAAGGAGCTGCACATGAAACTGCTGGTGGTCGAGGACGAAGCCCTGCTTCGCCATCACCTCTACACCCGCCTGGGCGAAAGCGGCCATGTG‑GTCGAGGCgg                                                                                       <  1:108573/149‑1 (MQ=255)
                                                          tgGGAGCGGGCGTGCCCGCGAAAGGGCCGGTACAGGCAATACACATAAGGAGCTGCACATGAAACTGCTGGTGGTCGAGGACGAAGCCCTGCTTCGCCATCACCTCTACACCCGCCTGGGCGAAAGCGGCCATGTG‑GTCGAGGCGGTCg                                                                                    >  7:71325/1‑149 (MQ=255)
                                                            ggAGCGGGCGTGCCCGCGAAAGGGCCGGTACAGGCAATACACATAAGGAGCTGCACATGAAACTGCTGGTGGTCGAGGACGAAGCCCTGCTTCGCCATCACCTCTACACCCGCCTGGGCGAAAGCGGCCATGTG‑GTCGAGGCGGTCGc                                                                                   <  7:199043/148‑1 (MQ=255)
                                                              aGCGGGCGTGCCCGCGAAAGGGCCGGTACAGGCAATACACATAAGGAGCTGCACATGAAACTGCTGGTGGTCGAGGACGAAGCCCTGCTTCGCCATCACCTCTACACCCGCCTGGGCGAAAGCGGCCATGTG‑GTCGAGGCGGTCGCTGa                                                                                <  3:193087/149‑1 (MQ=255)
                                                              aGCGGGCGTGCCCGCGAAAGGGCCGGTACAGGCAATACACATAAGGAGCTGCACATGAAACTGCTAGTGGTCGAGGACGAAGCCCTGCTTCGCCATCACCTCTACACCCGCCTGGGCGAAAGCGGCCATGTG‑GTCGAGGCGGTCGCTGa                                                                                <  8:199292/149‑1 (MQ=255)
                                                                cGGGCGTGCCCGCGAAAGGGCCGGTACAGGCAATACACATAAGGAGCTGCACATGAAACTGCTGGTGGTCGAGGACGAAGCCCTGCTTCGCCATCACCTCTACACCCGCCTGGGCGAAAGCGGCCATGTG‑GTCGAGGCGGTCGCTGATg                                                                              <  8:71325/149‑1 (MQ=255)
                                                                       gCCCGCGAAAGGGCCGGTACAGGCAATACACATAAGGAGCTGCACATGAAACTGCTGGTGGTCGAGGACGAAGCCCTGCTTCGCCATCACCTCTACACCCGCCTGGGCGAAAGCGGCCATGTG‑GTCGAGGCGGTCGCTGATGCCGaa                                                                         >  5:236836/1‑147 (MQ=255)
                                                                       gCCCGCGAAAGGGCCGGTACAGGCAATACACATAAGGAGCTGCACATGAAACTGCTGGTGGTCGAGGACGAAGCCCTGCTTCGCCATCACCTCTACACCCGCCTGGGCGAAAGCGGCCATGTG‑GTCGAGGCGGTCGCTGATGCCGAaga                                                                       <  3:169882/149‑1 (MQ=255)
                                                                        cccGCGAAAGGGCCGGTACAGGCAATACACATAAGGAGCTGCACATGAAACTGCTGGAGGTCGAGGACGAAGCCCTGCTTCGCCATCACCTCTACACCCGCCTGGGCGAAAGCGGCCATGTG‑GTCGAGGCGGTCGCTGATGCCGAagag                                                                      >  4:73429/1‑149 (MQ=255)
                                                                          cgcgAAAGGGCCGGTACAGGCAATACACATAAGGAGCTGCACATGAAACTGCTGGTGGTCGAGGACGAAGCCCTGCTTCGCCATCACCTCTACACCCGCCTGGGCGAAAGCGGCCATGTG‑GTCGAGGCTGTCGCTGATGCCg                                                                           <  6:193537/142‑1 (MQ=255)
                                                                          cgcgAAAGGGCCGGTACAGGCAATACACATAAGGAGCTGCACATGAAACTGCTGGTGGTCGAGGACGAAGCCCTGCTTCGCCATCACCTCTACACCCGCCTGGGCGAAAGCGGCCATGTG‑GTCGAGGCGGTCGCTGATGCCg                                                                           >  5:193537/1‑142 (MQ=255)
                                                                           gcgAAAGGGCCGGTACAGGCAATACACATAAGGAGCTGCACATGAAACTGCTGGTGGTCGAGGACGAAGCCCTGCTTCGCCATCACCTCTACACCCGCCGGGGCGAAAGCGGCCATGTG‑GTCGAGGCGGTCGCTGATGCCGAAGAGgcg                                                                   <  5:94462/149‑1 (MQ=255)
                                                                             gAAAGGGCCGGTACAGGCAATACACATAAGGAGCTGCACATGAAACTGCTGGTGGTCGAGGACGAAGCCCTGCTTCGCCATCACCTCTACACCCGCCTGGGCGAAAGCGGCCATGTG‑GTCGAGGCGGTCGCTGATGCCGAAGAGGCGCt                                                                 >  5:60784/1‑149 (MQ=255)
                                                                             gAAAGGGCCGGTACAGGCAATACACATAAGGAGCTGCACATGAAACTGCTGGTGGTCGAGGACGAAGCCCTGCTTCGCCATCACCTCTACACCCGCCTGGGCGAAAGCGGCCATGTG‑GTCGAGGCGGTCGCTGATGCCGAAGAGGCGCt                                                                 >  4:60111/1‑149 (MQ=255)
                                                                             gAAAGGGCCGGTACAGGCAATACACATAAGGAGCTGAACATGAAACTGCTGGTGGTCGAGGACGAAGCCCTGCTTCGCCATCACCTCTACACCCGCCTGGGCGAAAGCGGCCATGTG‑GTCGAGGCGGTCGCTGATGCCGAAGAGGCGCt                                                                 <  2:173726/149‑1 (MQ=255)
                                                                                   gCCGGTACAGGCAATACACATAAGGAGCTGCACATGAAACTGCTGGTGGTCGAGGACGAAGCCCTGCTTCGCCATCACCTCTACACCCGCCTGGGCGAAAGCGGCCATGTG‑GTCGAGGCGGTCGCTGATGCCGAAGAGGCGCTGTAcc                                                            <  6:236836/148‑1 (MQ=255)
                                                                                   gCCGGTACAGGCAATACACATAAGGAGCTGCACATGAAACTGCTGGTGGTCGAGGACGAAGCCCTGCTTCGCCATCACCTCTACACCCGCCTGGGCGAAAGCGGCCATGTG‑GTCGAGGCGGTCGCTGATGCCGAAGAGGCGCTGTACCa                                                           <  6:97613/149‑1 (MQ=255)
                                                                                             gCAATACACATAAGGAGCTGCACATGAAACTGCTGGTGGTCGAGGACGAAGCCCTGCTTCGCCATCACCTCTACACCCGCCTGGGCGAAAGCGGCCATGTG‑GTCGAGGCGGTCGCTGATGCCGAAGAGGCGCTGTACCAGGCCGAGCAg                                                 >  7:123855/1‑149 (MQ=255)
                                                                                                 tACACATAAGGAGCTGCACATGAAACTGCTGGTGGTCGAGGACGAAGCCCTGCTTCGCCATCACCTCTACACCCGCCTGGGCGAAAGCGGCCATGTG‑GTCGAGGCGGTCGCTGATGCCGAAGAGGCGCTGTACCAGGCCGAGCAGTAcc                                             <  3:60111/149‑1 (MQ=255)
                                                                                                      aTAAGGAGCTGCACATGAAACTGCTGGTGGTCGAGGACGAAGCCCTGCTTCGCCATCACCTCTACACCCGCCTGGGCGAAAGCGGCCATGTGATTCGAGGCGGTCGCTGATGCCGAAGAGGCGCTGTACCAGGCCGAGCAGTACCAttt                                         <  1:74081/149‑1 (MQ=255)
                                                                                                      aTAAGGAGCTGCACATGAAACTGCTGGTGGTCGAGGACGAAGCCCTGCTTCGCCATCACCTCTACACCCGCCTGGGCGAAAGCGGCCATGTG‑GTCGAGGCGGTCGCTGATGCCGAAGAGGCGCTGTACCAGGCCGAGCAGTACCATTTc                                        <  4:205476/149‑1 (MQ=255)
                                                                                                      aTAAGGAGCTGCACATGAAACTGCTGGTGGTCGAGGACGAAGCCCTGCTTCGCCATCACCTCTACACCCGCCTGGGCGAAAGCGGCCATGTG‑GTCGAGGCGGTCGCTGATGCCGAAGAGGCGCTGTACCAGGCCGAGCAGTACCATTTc                                        >  3:205476/1‑149 (MQ=255)
                                                                                                        aaGGAGCTGCACATGAAACTGCTGGTGGTCGAGGACGAAGCCCTGCTTCGCCATCACCTCTACACCCGCCTGGGCGAAAGCGGCCATGTG‑GTCGAGGCGGTCGCTGATGCCGAAGAGGCGCTGTACCAGGCCGAGCAGTACCATTTCGa                                      >  5:197630/1‑149 (MQ=255)
                                                                                                         aGGAGCTGCACATGAAACTGCTGGTGGTCGAGGACGAAGCCCTGCTTCGCCATCACCTCTACACCCGCCTGGGCGAAAGTGGCCATGTG‑GTCGAGGCGGTCGCTGATGCCGAAGAGGCGCTGTACCAGGCCGAGCAGTACCATTTCGa                                      <  6:53960/148‑1 (MQ=255)
                                                                                                              cTGCACATGAAACTGCTGGTGGTCGAGGACGAAGCCCTGCTTCGCCATCACCTCTACACCCGCCTGGGCGAAAGCGGCCATGTG‑GTCGAGGCGGTCGCTGATGCCGAAGAGGCGCTGTACCAGGCCGAGCAGTACCATTTCGACCTTGc                                <  8:210055/149‑1 (MQ=255)
                                                                                                                    aTGAAACTGCTGGTGGTCGAGGACGAAGCCCTGCTTCGCCATCACCTCTACACCCGCCTGGGCGAAAGCGGCCATGTG‑GTCGAGGCGGTCGCTGATGCCGAAGAGGCGCTGTACCAGGCCGAGCAGTACCATTTCGACCTTGCcatcat                          >  8:13149/1‑149 (MQ=255)
                                                                                                                     tGAAACTGCTGGTGGTCGAGGACGAAGCCCTGCTTCGCCATCACCTCTACACCCGCCTGGGCGAAAGCGGCCATGTG‑GTCGAGGCGGTCGCTGATGCCGAAGAGGCGCTGTACCAgg                                                         <  5:144050/117‑1 (MQ=255)
                                                                                                                     tGAAACTGCTGGTGGTCGAGGACGAAGCCCTGCTTCGCCATCACCTCTACACCCGCCTGGGCGAAAGCGGCCATGTG‑GTCGAGGCGGTCGCTGATGCCGAAGAGGCGCTGTACCAgg                                                         >  6:144050/1‑117 (MQ=255)
                                                                                                                      gAAACTGCTGGTGGTCGAGGACGAAGCCCTGCTTCGCCATCACCTCTACACCCGCCTGGGCGAAAGCGGCCATGTG‑GTCGAGGCGGTCGCTGATGCCGAAGAGGCGCTGTACCAGGCCGAGCAGTACCATTTCGACCTTGCCatcatc                         >  8:99431/1‑148 (MQ=255)
                                                                                                                       aaaCTGCTGGTGGTCGAGGACGAAGCCCTGCTTCGCCATCACCTCTACACCCGCCTGGGCGAAAGCGGCCATGTG‑GTCGAGGCGGTCGCTGATGCCGAAGAGGCGCTGTACCAGGCCGAGCAGTACCATTTCGACCTTGCCATCATCGa                       >  7:47322/1‑149 (MQ=255)
                                                                                                                         aCTGCTGGTGGTCGAGGACGAAGCCCTGCTTCGCCATCACCTCTACACCCGCCTGGGCGAAAGCGGCCATGTG‑GTCGAGGCGGTCGCTGATGCCGAAGAGCCGCTGTACCAGGCCGAGCAGTACCATTTCGACCTTGCCATCATCGAc                      >  8:192015/1‑148 (MQ=255)
                                                                                                                           tgctgGTGGTCGAGGACGAAGCCCTGCTTCGCCATCACCTCTACACCCGCCTGGGCGAAAGCGGCCATGTG‑GTCGAGGCGGTCGCTGATGCCGAAGAGGCGCTGTACCAGGCCGAGCAGTACCATTTCGACCTTGCCATCATCGACCTg                   >  6:231228/1‑149 (MQ=255)
                                                                                                                              tggtggTCGAGGACGAAGCCCTGCTTCGCCATCACCTCTACACCCGCCTGGGCGAAAGCGGCCATGTG‑GTCGAGGCGGTCGCTGATGCCGAAGAGGCGCTGTACCAGGCCGAGCAGTACCATTTCGACCTTGCCATCATCGACCTGGGc                >  4:206168/1‑149 (MQ=255)
                                                                                                                                 tggtCGAGGACGAAGCCCTGCTTCGCCATCACCTCTACACCCGCCTGGGCGAAAGCGGCCATGTG‑GTCGAGGCGGTCGCTGATGCCGAAGAGGCGCTGTACCAGGCCGAGCAGTACCATTTCGACCTTGCCATCATCGACCTGGGCCTg             <  8:47322/149‑1 (MQ=255)
                                                                                                                                           cGAAGCCCTGCTTCGCCATCACCTCTACACCCGCCTGGGCGAAAGCGGCCATGTG‑GTCGAGGCGGTCGCTGATGCCGAAGAGGCGCTGTACCAGGCCGAGCAGTACCATTTCGACCTTGCCATCATCGACCTGGGCCTGCCCGGTATCa   <  7:99431/149‑1 (MQ=255)
                                                                                                                                            gAAGCCCTGCTTCGCCATCACCTCTACACCCGCCTGGGCGAAAGCGGCCATGTG‑GTCGAGGCGGTCGCTGATGCCGAAGAGGCGCTGTACCAGGCCGAGCAGTACCATTTCGACCTTGCCATCATCGACCTGGGCCTGCCCGGTATCAg  >  3:89405/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                               
TGCAGTGCCTTGGGTTTCTGCAGGTGCTACACAGGTTTCAGGGACTGTGCGATTACTGTGGGAGCGGGCGTGCCCGCGAAAGGGCCGGTACAGGCAATACACATAAGGAGCTGCACATGAAACTGCTGGTGGTCGAGGACGAAGCCCTGCTTCGCCATCACCTCTACACCCGCCTGGGCGAAAGCGGCCATGTG‑GTCGAGGCGGTCGCTGATGCCGAAGAGGCGCTGTACCAGGCCGAGCAGTACCATTTCGACCTTGCCATCATCGACCTGGGCCTGCCCGGTATCAG  >  NC_002947/1360821‑1361109

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑