Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,377,539 G→C 7.1% P32A (CCC→GCC)  PP_1201 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,377,5390GC 7.1% 169.5 / 4.0 56P32A (CCC→GCC) PP_1201hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (27/25);  new base C (2/2);  total (29/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.52e-01

CGGCCTGGCTCAGGTCGAGCAGTTGCTTGCCCGTCAGTGCCTGGTCTTGTGGCACGCCGATGGCCACCCGGGCCTTGGGGTGCACGCCAGGGGTAACACGGGTGATGCTGGTGTCATTGATGCTCAACTGTTCCAGGCGCGCCTCGGGGGCCGGGCGCGTCCACAGCCAGAAGGCCAGCGCACCACCCAGGGCGGCCAGCAGCAGGGGAACCAGCAGGTACAACAAAAAGCGTCGGGTCATCAGCGTTTCACCAATCCAGTCAGGCCGCCTGCAATCAGGGCGGCGGTGTCG  >  NC_002947/1377392‑1377683
                                                                                                                                                   |                                                                                                                                                
cGGCCTGGCTCAGGTCGAGCAGTTGCTTGCCCGTCAGTGCCTGGTCTTGAGGCACGCCGATGGCCACCCGGGCCTTGGGGTGCACGCCAGGGGTAACACGGGTGATGCTGGTGTCATTGATGCTCAACTGTTCCAGGCGCGCCTCgggg                                                                                                                                                 >  1:63278/1‑149 (MQ=255)
       gCTCAGGTCGAGCAGTTGCTTGCCCGTCAGTGCCTGGTCTTGTGGCTCGCCGATGGCCACCCGGGCCTTGGGGTGCACGCCAGGGGTAACACGGGTGATGCTGGTGTCATTGATGCTCAACTGTTCCAGGCGCGCCTCGGGGGCCGggc                                                                                                                                          >  1:89115‑M2/1‑146 (MQ=255)
           aGGTCGAGCAGTTGCTTGCCCGTCAGTGCCTGGTCTTGTGGCACGCCGATGGCCACCCGGGCCTTGGGGTGCACGCCAGGGGTAACACGGGTGATGCTGGTGTCATTGATGCTCAACTGTTCCAGGCGCGCCTCGGGGGCCGggcgcg                                                                                                                                       >  5:28925‑M2/1‑142 (MQ=255)
             gTCGAGCAGTTGCTTGCCCGTCAGTGCCTGGTCTTGTGGCACGCCGATGGCCACCCGGGCCTTGGGGTGCACGCCAGGGGTAACACGGGTGATGCTGGTGTCATTGATGCTCAACTGTTCCAGGCGCGCCTCGGGGGCCGg                                                                                                                                            <  4:113526‑M2/141‑2 (MQ=255)
             gTCGAGCAGTTGCTTGCCCGTCAGTGCCTGGTCTTGTGGCACGCCGATGGCCACCCGGGCCTTGGGGTGCACGCCAGGGGTAACACGGGTGATGCTGGTGTCATTGATGCTCAACTGTTCCAGGCGCGCCTCGGGGGCCGg                                                                                                                                            >  3:113526‑M2/1‑140 (MQ=255)
                        gcttgcCCGTCAGTGCCTGGTCTTGTGGCACGCCGATGGCCACCCGGGCCTTGGGGTGCACGCCAGGGGTAACACGGGTGATGCTGGTGTCATTGATGCTCAACTGTTCCAGGCGCGCCTCGGGGGCCGGGCGCGTCCACAGCCAGAAg                                                                                                                         >  1:260404/1‑149 (MQ=255)
                          ttgcCCGTCAGTGCCTGGTCTTGTGGCACGCCGATGGCCACCCGGGCCTTGGGGTGCACGCCAGGGGTAACACGGGTGATGCTGGTGTCATTGATGCTCAACTGTTCCAGGCGCGCCTCGGGGGCCGGGCGCGTCCACAGCCAGAAGGc                                                                                                                       >  1:145785/1‑149 (MQ=255)
                            gcCCGTCAGTGCCTGGTCTTGTGGCACGCCGATGGCCACCCGGGCCTTGGGGTGCACGCCAGGGGTAACACGGGTGATGCTGGTGTCATTGATGCTCAACTGTTCCAGGCGCGCCTCGGGGGCCGGGCGCGTCCACAGCCAGAAGGc                                                                                                                       <  2:79237/147‑1 (MQ=255)
                                gTCAGTGCCTGGTCTTGTGGCACGCCGATGGCCACCCGGGCCTTGGGGTGCACGCCAGGGGTAACACGGGTGATGCTGGTGTCATTGATGCTCAACTGTTCCAGGCGCGCCTCGGGGGCCGGGCGCGTCCACAGCCAGAAGGCCAgcgc                                                                                                                 <  6:138616/149‑1 (MQ=255)
                                    gTGCCTGGTCTTGTGGCACGCCGATGGCCACCCGGGCCTTGGGGTGCACGCCAGGGGTAACACGGGTGATGCTGGTGTCATTGATGCTCAACTGTTCCAGGCGCGCCTCGGGGGCCGGGCGCGTCCACAGCCAGAAGGCCAGCGcacca                                                                                                             >  7:251613/1‑149 (MQ=255)
                                    gTGCCTGGTCTTGTGGCACGCCGATGGCCACCCGGGCCTTGGGGTGCACGCCAGGGGTAACACGGGTGATGCTGGTGTCATTGATGCTCAACTGTTCCAGGCGCGCC‑CGGCCCCCGaggcgcgcctggaacagttga                                                                                                                        <  5:272709‑M2/137‑22 (MQ=255)
                                    gTGCCTGGTCTTGTGGCACGCCGATGGCCACCCGGGCCTTGGGGTGCACGCCAGGGGTAACACGGGTGATGCTGGTGTCATTGATGCTCAACTGTTCCAGGCGCGCC‑CGGCCCCCGaggcgcgcctggaacagttga                                                                                                                        >  6:272709‑M2/1‑116 (MQ=255)
                                        cTGGTCTTGTGGCACGCCGATGGCCACCCGGGCCTTGGGGTGCACGCCAGGGGTAACACGGGTGATGCTGGTGTCATTGATGCTCAACTGTTCCAGGCGCGCCTCGGGGGCCGGGCGCGTCCACAGCCAGAAGGCCAGCGCACCACCCa                                                                                                         <  5:236318/149‑1 (MQ=255)
                                              ttGTGGCACGCCGATGGCCACCCGGGCCTTGGCGTGCACGCCAGGGGTAACACGGGTGATGCTGGTGTCATTGATGCTCAACTGTTCCAGGCGCGCCTCGGGGGCCGGGCGCTTCAACAGCGAGACGGCCCGCCCCCCACCCCGggcgg                                                                                                   >  8:233562/1‑149 (MQ=255)
                                                gtgGCACGCCGATGGCCACCCGGGCCTTGGGGTGCACGCCAGGGGTAACACGGGTGATGCTGGTGTCATTGATGCTCAACTGTTCCAGGCGCGCCTCGGGGGCCGGGCGCGTCCACAGCCAGAAGGCCa                                                                                                                     <  8:202928/129‑1 (MQ=255)
                                                gtgGCACGCCGATGGCCACCCGGGCCTTGGGGTGCACGCCAGGGGTAACACGGGTGATGCTGGTGTCATTGATGCTCAACTGTTCCAGGCGCGCCTCGGGGGCCGGGCGCGTCCACAGCCAGAAGGCCa                                                                                                                     >  7:202928/1‑129 (MQ=255)
                                                   gCACGCCGATGGCCACCCGGGCCTTGGGGTGCACGCCAGGGGTAACACGGGTGATGCTGGTGTCATTGATGCTCAACTGTTCCAGGCGCGCCTCGGGGGCCGGGCGCGTCCACAGCCAGAAGGCCAGCGCACCACCCAGGGCGGCcag                                                                                               <  7:53286/148‑1 (MQ=255)
                                                     aCGCCGATGGCCACCCGGGCCTTGGGGTGCACGCCAGGGGTAACACGGGTGATGCTGGTGTCATTGATGCTCAACTGTTCCAGGCGCGCCTCGGGGGCCGGGCGCGTCCACAGCCAGAAGGCCAGCGCACCACCCAGGGCGGCcagcag                                                                                            >  1:134351/1‑149 (MQ=255)
                                                           aTGGCCACCCGGGCCTTGGGGTGCACGCCAGGGGTAACACGGGTGATGCTGGTGTCATTGATGCTCAACTGTTCCAGGCGCGCCTCGGGGGCCGGGCGCGTCCACAGCCAGAAGGCCAGCGCACCACCCAGGGCGGCCAGCAGCAgg                                                                                        >  8:194450/1‑147 (MQ=255)
                                                           aTGGCCACCCGGGCCTTGGGGTGCACGCCAGGGGTAACACGGGTGATGCTGGTGTCATTGATGCTCAACTGTTCCAGGCGCGCCTCGGGGGCCGGGCGCGTCCACAGCCAGAAGGCCAGCGCACCACCCAGGGCGGCCAGCAGCAgg                                                                                        <  7:194450/147‑1 (MQ=255)
                                                              gCCACCCGGGCCTTGGGGTGCACGCCAGGGGTAACACGGGTGATGCTGGTGTCATTGATGCTCAACTGTTCCAGGCGCGCCTCGGGGGCCGGGCGCGTCCACAGCCAGAAGGCCAGCGCACCACCCAGGGCGGCCAGCAGCAGGGGAAc                                                                                   <  2:63278/149‑1 (MQ=255)
                                                               ccACCCGGGCCTTGGGGTGCACGCCAGGGGTAACACGGGTGATGCTGGTGTCATTGATGCTCAACTGTTCCAGGCGCGCCTCGGGGGCCGGGCGCGTCCACAGCCAGAAGGCCAGCGCACCACCCAGGGCGGCCAGCAGCAGGGGAAc                                                                                   >  6:23452/1‑148 (MQ=255)
                                                               ccACCCGGGCCTTGGGGTGCACGCCAGGGGTAACACGGGTGATGCTGGTGTCATTGATGCTCAACTGTTCCAGGCGCGCCTCGGGGGCCGGGCGCGTCCACAGCCAGAAGGCAAGCGCACCACCCAGGGCGGCCAGCAGCAGGGGAAc                                                                                   >  7:121566/1‑148 (MQ=255)
                                                                   ccGGGCCTTGGGGTGCACGCCAGGGGTAACACGGGTGATGCTGGTGTCATTGATGCTCAACTGTTCCAGGCGCGCCTCGGGGGCCGg                                                                                                                                            >  2:154477‑M2/1‑86 (MQ=255)
                                                                   ccGGGCCTTGGGGTGCACGCCAGGGGTAACACGGGTGATGCTGGTGTCATTGATGCTCAACTGTTCCAGGCGCGCCTCGGGGGCCGg                                                                                                                                            <  1:154477‑M2/87‑2 (MQ=255)
                                                                   ccGGGCCTTGGGGTGCACGCCAGGGGTAACACGGGTGATGCTGGTGTCATTGATGCTCAACTGTTCCAGGCGCGCCTCGGGGGCCGGGCGCGTCCACAGCCAGAAGGCCAGCGCACCACCCAGGGCGGCCAGCAGCAGGGGAACcagca                                                                              <  4:202123/149‑1 (MQ=255)
                                                                   ccGGGCCTTGGGGTGCACGCCAGGGGTAACACGGGTGATGCTGGTGTCATTGATGCTCAACTGTTCCAGGCGCGCCTCGGGGGCCGGGCGCGTCCACAGCCAGAAGGCCAGCGCACCACCCAGGGCGGCCAGCAGCAGGGGAACcagca                                                                              <  5:23452/149‑1 (MQ=255)
                                                                               gTGCACGCCAGGGGTAACACGGGTGATGCTGGTGTCATTGATGCTCAACTGTTCCAGGCGCGCCTCGGGGGCCGGGCGCGTCCACAGCCAGAAGGCCAGCGCACCACCCAGGGCGGCCAGCAGCAGGGGAACCAGCAGGTACAACaaa                                                                   >  6:126511/1‑148 (MQ=255)
                                                                                tGCACGCCAGGGGTAACACGGGTGATGCTGGTGTCATTGATGCTCAACTGTTCCAGGCGCGCCTCGGGGGCCGGGCGCGTCCACAGCCAGAAGGCCAGCGcac                                                                                                               >  7:121295/1‑103 (MQ=255)
                                                                                tGCACGCCAGGGGTAACACGGGTGATGCTGGTGTCATTGATGCTCAACTGTTCCAGGCGCGCCTCGGGGGCCGGGCGCGTCCACAGCCAGAAGGCCAGCGcac                                                                                                               <  8:121295/103‑1 (MQ=255)
                                                                                  cACGCCAGGGGTAACACGGGTGATGCTGGTGTCATTGATGCTCGACTGTTCCAGGCGCGCC‑CGGCCCCCGaggcgcgcctggaac                                                                                                                              <  2:196915‑M2/85‑16 (MQ=255)
                                                                                  cACGCCAGGGGTAACACGGGTGATGCTGGTGTCATTGATGCTCGACTGTTCCAGGCGCGCC‑CGGCCCCCGaggcgcgcctggaac                                                                                                                              >  1:196915‑M2/1‑70 (MQ=255)
                                                                                      ccAGGGGTAACACGGGTGATGCTGGTGTCATTGATGCTCAACTGTTCCAGGCGCGCCTCGGGGGCCGGGCGCGTCCACAGCCAGAAGGCCAGCGCACCACCCAGGGCGGCCAGCAGCAGGGGAACCAGCAGGTACAACAAAAAGCGTCg                                                           <  5:126511/149‑1 (MQ=255)
                                                                                       cAGGGGTAACACGGGTGATGCTGGTGTCATTGATGCTCAACTGTTCCAGGCGCGCCTCGGGGGCCGGGCGCGTCCACAGCCAGAAGGCCAGCGCACCACCCAGGGCGGCCAGCAGCAgggg                                                                                      >  2:181587/1‑121 (MQ=255)
                                                                                       cAGGGGTAACACGGGTGATGCTGGTGTCATTGATGCTCAACTGTTCCAGGCGCGCCTCGGGGGCCGGGCGCGTCCACAGCCAGAAGGCCAGCGCACCACCCAGGGCGGCCAGCAGCAgggg                                                                                      <  1:181587/121‑1 (MQ=255)
                                                                                                cacGGGTGATGCTGGTGTCATTGATGCTCAACTGTTCCAGGCGCGCCTCGGGGGCCGGGCGCGTCCACAGCCAGAAGGCCAGCGCACCACCCAGGGCGGCCAGCAGCAGGGGAACCAGCAGGTACAACAAAAAGCGTCGGGTCATCAGc                                                 >  1:204032/1‑149 (MQ=255)
                                                                                                      tGATGCTGGTGTCATTGATGCTCAACTGTTCCAGGCGCGCCTCGGGGGCCGGGCGCGTCCACAGCCAGAAGGCCAGCGCACCACCCAGGGCGGCCAGCAGCAGGGGAACCAGCAGGTACAACAAAAAGCGTCGGGTCATCAGCGTTTCa                                           <  2:134351/149‑1 (MQ=255)
                                                                                                        aTGCTGGTGTCATTGATGCTCAACTGTTCCAGGCGCGCCTCGGGGGCCGGGCGCGTCCACAGCCAGAAGGCCAGCGCACCACCCAGGGCGGCCAGCAGCAGGGGAACCAGCAGGTACAACAAAAAGCGTCGGGTCATCAGCGTTTCAc                                          <  8:121566/148‑1 (MQ=255)
                                                                                                            tGGTGTCATTGATGCTCAACTGTTCCAGGCGCGCCTCGGGGGCCGGGCGCGTCCACAGCCAGAAGGCCAGCGCACCACCCAGGGCGGCCAGCAGCAGGGGAACCAGCAGGTACAACAAAAAGCGTCGGGTCATCAGCGTTTCACCAATc                                     >  5:255555/1‑149 (MQ=255)
                                                                                                                gtCATTGATGCTCAACTGTTCCAGGCGCGCCTCGGGGGCCGGGCGCGTCCACAGCCAGAAGGCCAGCGCACCACCCAGGGCGGCCAGCAGCAGGGGAACCAGCAGGTACAACAAAAAGCGTCGGGTCATCAGCGTTTCACCAATCCAGt                                 >  3:130846/1‑149 (MQ=255)
                                                                                                                   aTTGATGCTCAACTGTTCCAGGCTCGCCTCGGGGGCCGGGCGCGGACACATCCAGAATGCCAGCGCACCACCCAGGGCGGCCAGCAGCAGGGTAACCAGCAGGTACAACAAAAAGCGTCGGGTCATCAGCGTTTCACCAATCCAGTCAg                              <  7:233562/149‑1 (MQ=255)
                                                                                                                   aTTGATGCTCAACTGTTCCAGGCGCGCCTCGGGGGCCGGGCGCGTCCACAGCCAGAAGGCCAGCGCACCACCCAGggcg                                                                                                    >  8:247336/1‑79 (MQ=255)
                                                                                                                   aTTGATGCTCAACTGTTCCAGGCGCGCCTCGGGGGCCGGGCGCGTCCACAGCCAGAAGGCCAGCGCACCACCCAGggcg                                                                                                    <  7:247336/79‑1 (MQ=255)
                                                                                                                          cTCAACTGTTCCAGGCGCGCCTCGGGGGCCGGGCGCGTCCACAGCCATAAGGCCAGCGCACCACCCAGGGCGGCCAGCAGCAGGGTAACCAGCAGGTACAACAAAAAGCGTCGGGTCATCAGCGTTTCACCAATCCAGTCAGGCCGCCt                       <  2:260404/149‑1 (MQ=255)
                                                                                                                          cTCAACTGTTCCAGGCGCGCCTCGGGGGCCGGGCGCGTCCACAGCCAGAAGGCCAGCGCACCACCCAGGGCGGCCAGCAGCAGGGGAACCAGCAGGTACAACAAAAAGCGTCGGGTCATCAGCGTTTCACCAATCCAGTCAGGCCGCCt                       >  6:232993/1‑149 (MQ=255)
                                                                                                                             aaCTGTTCCAGGCGCGCCTCGGGGGCCGGGCGCGTCCACAGTCAGAAGGCCAGCGCACCACCCAGGGCGGCCAGCAGTAGGGGAACCAGCAGGTACAACAAAAAGCGTCGGGTCATCAGCGTTTCACCAATCCAGTCAGGCCGCCTGc                     >  8:188243/1‑148 (MQ=255)
                                                                                                                                  ttCCAGGCGCGCCTCGGGGGCCGGGCGCGTCCACAGCCAGAAGGCCAGCGCACCACCCAGGGCGGCCAGCAGCAGGGGAACCAGCAGGTACAACAAAAAGCGTCGGGTCATCAGCGTTTCACCAATCCAGTCAGGCCGCCTGCAATCa                <  8:251613/148‑1 (MQ=255)
                                                                                                                                   tCCAGGCGCGCCTCGGGGGCCGGGCGCGTCCACAGCCAGAAGGCCAGCGCACCACCCAGGGCGGCCAGCAGCAGGGGAACCAGCAGGTACAACAAAAAGCGTCGGGTCATCAGCGTTTCACCAATCCAGTCAGGCCGCCTGCAATCAgg              >  8:151758/1‑149 (MQ=255)
                                                                                                                                    ccAGGCGCGCCTCGGGGGCCGGGCGCGTCCACAGCCAGAAGGCCAGCGCACCACCCAGGACGGCCAGCAGCAGGGGAACCAGCAGGTACAACAAAAAGCGTCGGGTCATCAGCGTTTCACCAATCCAGTCAGGCCGCCt                       >  3:237151/1‑139 (MQ=255)
                                                                                                                                    ccAGGCGCGCCTCGGGGGCCGGGCGCGTCCACAGCCAGAAGGCCAGCGCACCACCCAGGACGGCCAGCAGCAGGGGAACCAGCAGGTACAACAAAAAGCGTCGGGTCATCAGCGTTTCACCAATCCAGTCAGGCCGCCt                       <  4:237151/139‑1 (MQ=255)
                                                                                                                                        gcgcgcCTCGGGGGCCGGGCGCGTCCACAGCCAGAAGGCCAGCGCACCACCCAGGGCGGCCAGCAGCAGGGGAACCAGCAGGTACAACAAAAAGCGTCGGGTCATCAGCGTTTCACCAATCCAGTCAGGCCGCCTGCAATCAGggcggc         <  4:130846/149‑1 (MQ=255)
                                                                                                                                         cgcgcCTCGGGGGCCGGGCGCGTCCACAGCCAGAAGGCCAGCGCACCACCCAGGGCGGCCAGCAGCAGGGGAACCAGCAGGTACAACAAAAAGCGTCGGGTCATCAGCGTTTCACCAATCCAGTCAGGCCGCCTGCAATCAGGgcggcg        <  2:145785/149‑1 (MQ=255)
                                                                                                                                            gcCTCGGGGGCCGGGCGCGTCCACAGCCAGAAGGCCAGCGCACCACCCAGGGCGGCCAGCAGCAGGGGAACCAGCAGGTACAACAAAAAGCGTCGGGTCATCAGCGTTTCACCAATCCAGTCAGGCCGCCTGCAATCAGGGCGGCGgtg     >  3:122592/1‑149 (MQ=255)
                                                                                                                                            gcCTCGGGGGCCGGGCGCGTCCACAGCCAGAAGGCCAGCGCACCACCCAGGGCGGCCAGCAGCAGGGGAACCAGCAGGTACAACAAAAAGCGTCGGGTCATCAGCGTTTCACCAATCCAGTCAGGCCGCCTGCAATCAGGGCGGCGgtg     <  6:255555/149‑1 (MQ=255)
                                                                                                                                              cTCGGGGGCCGGGCGCGTCCACAGTCAGAAGGCCAGCGCACCACCCAGGGCGGCCAGCAGTAGGGGAACCAGCAGGTACAACAAAAAGCGTCGGGTCATCAGCGTTTCACCAATCCAGTCAGGCCGCCTGCAATCAGGGCGGCGGTGTc   <  7:188243/149‑1 (MQ=255)
                                                                                                                                              cTCGGGGGCCGGGCGCGTCCACAGCCAGAAGGCCAGCGCACCACCCAGGGCGGCCAGCAGCAGGGGAACCAGCAGGTACAACAAAAAGCGTCGGGTCATCAGCGTTTCACCAATCCAGTCAGGCCGCCTGCAATCAGGGCGGCGGTGTc   >  7:127094/1‑149 (MQ=255)
                                                                                                                                               tCGGGGGCCGGGCGCGTCCACAGCCAGAAGGCCAGCGCACCACCCAGGGCGGCCAGCAGCAGGGGAACCAGCAGGTACAACAAAAAGCGTCGGGTCATCAGCGTTTCACCAATCCAGTCAGGCCGCCTGCAATCAGGGCGGCGGTGTCg  >  6:54446/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                
CGGCCTGGCTCAGGTCGAGCAGTTGCTTGCCCGTCAGTGCCTGGTCTTGTGGCACGCCGATGGCCACCCGGGCCTTGGGGTGCACGCCAGGGGTAACACGGGTGATGCTGGTGTCATTGATGCTCAACTGTTCCAGGCGCGCCTCGGGGGCCGGGCGCGTCCACAGCCAGAAGGCCAGCGCACCACCCAGGGCGGCCAGCAGCAGGGGAACCAGCAGGTACAACAAAAAGCGTCGGGTCATCAGCGTTTCACCAATCCAGTCAGGCCGCCTGCAATCAGGGCGGCGGTGTCG  >  NC_002947/1377392‑1377683

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑