Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,377,541 G→C 7.1% A31G (GCC→GGC) ‡ PP_1201 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,377,5410GC 7.1% 176.1 / 4.0 56A31G (GCC→GGC) ‡PP_1201hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (27/25);  new base C (2/2);  total (29/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.71e-01

GCTCAGGTCGAGCAGTTGCTTGCCCGTCAGTGCCTGGTCTTGTGGCACGCCGATGGCCACCCGGGCCTTGGGGTGCACGCCAGGGGTAACACGGGTGATGCTGGTGTCATTGATGCTCAACTGTTCCAGGCGCGCCTCGGGGGCCGGGCGCGTCCACAGCCAGAAGGCCAGCGCACCACCCAGGGCGGCCAGCAGCAGGGGAACCAGCAGGTACAACAAAAAGCGTCGGGTCATCAGCGTTTCACCAATCCAGTCAGGCCGCCTGCAATCAGGGCGGCGGTGTCGGCCAGT  >  NC_002947/1377399‑1377689
                                                                                                                                              |                                                                                                                                                    
gCTCAGGTCGAGCAGTTGCTTGCCCGTCAGTGCCTGGTCTTGTGGCTCGCCGATGGCCACCCGGGCCTTGGGGTGCACGCCAGGGGTAACACGGGTGATGCTGGTGTCATTGATGCTCAACTGTTCCAGGCGCGCCTCGGGGGCCGggc                                                                                                                                                >  1:89115‑M2/1‑146 (MQ=255)
    aGGTCGAGCAGTTGCTTGCCCGTCAGTGCCTGGTCTTGTGGCACGCCGATGGCCACCCGGGCCTTGGGGTGCACGCCAGGGGTAACACGGGTGATGCTGGTGTCATTGATGCTCAACTGTTCCAGGCGCGCCTCGGGGGCCGggcgcg                                                                                                                                             >  5:28925‑M2/1‑142 (MQ=255)
      gTCGAGCAGTTGCTTGCCCGTCAGTGCCTGGTCTTGTGGCACGCCGATGGCCACCCGGGCCTTGGGGTGCACGCCAGGGGTAACACGGGTGATGCTGGTGTCATTGATGCTCAACTGTTCCAGGCGCGCCTCGGGGGCCGg                                                                                                                                                  <  4:113526‑M2/141‑2 (MQ=255)
      gTCGAGCAGTTGCTTGCCCGTCAGTGCCTGGTCTTGTGGCACGCCGATGGCCACCCGGGCCTTGGGGTGCACGCCAGGGGTAACACGGGTGATGCTGGTGTCATTGATGCTCAACTGTTCCAGGCGCGCCTCGGGGGCCGg                                                                                                                                                  >  3:113526‑M2/1‑140 (MQ=255)
                 gcttgcCCGTCAGTGCCTGGTCTTGTGGCACGCCGATGGCCACCCGGGCCTTGGGGTGCACGCCAGGGGTAACACGGGTGATGCTGGTGTCATTGATGCTCAACTGTTCCAGGCGCGCCTCGGGGGCCGGGCGCGTCCACAGCCAGAAg                                                                                                                               >  1:260404/1‑149 (MQ=255)
                   ttgcCCGTCAGTGCCTGGTCTTGTGGCACGCCGATGGCCACCCGGGCCTTGGGGTGCACGCCAGGGGTAACACGGGTGATGCTGGTGTCATTGATGCTCAACTGTTCCAGGCGCGCCTCGGGGGCCGGGCGCGTCCACAGCCAGAAGGc                                                                                                                             >  1:145785/1‑149 (MQ=255)
                     gcCCGTCAGTGCCTGGTCTTGTGGCACGCCGATGGCCACCCGGGCCTTGGGGTGCACGCCAGGGGTAACACGGGTGATGCTGGTGTCATTGATGCTCAACTGTTCCAGGCGCGCCTCGGGGGCCGGGCGCGTCCACAGCCAGAAGGc                                                                                                                             <  2:79237/147‑1 (MQ=255)
                         gTCAGTGCCTGGTCTTGTGGCACGCCGATGGCCACCCGGGCCTTGGGGTGCACGCCAGGGGTAACACGGGTGATGCTGGTGTCATTGATGCTCAACTGTTCCAGGCGCGCCTCGGGGGCCGGGCGCGTCCACAGCCAGAAGGCCAgcgc                                                                                                                       <  6:138616/149‑1 (MQ=255)
                             gTGCCTGGTCTTGTGGCACGCCGATGGCCACCCGGGCCTTGGGGTGCACGCCAGGGGTAACACGGGTGATGCTGGTGTCATTGATGCTCAACTGTTCCAGGCGCGCCTCGGGGGCCGGGCGCGTCCACAGCCAGAAGGCCAGCGcacca                                                                                                                   >  7:251613/1‑149 (MQ=255)
                             gTGCCTGGTCTTGTGGCACGCCGATGGCCACCCGGGCCTTGGGGTGCACGCCAGGGGTAACACGGGTGATGCTGGTGTCATTGATGCTCAACTGTTCCAGGCGCGCC‑CGGCCCCCGaggcgcgcctggaacagttga                                                                                                                              <  5:272709‑M2/137‑22 (MQ=255)
                             gTGCCTGGTCTTGTGGCACGCCGATGGCCACCCGGGCCTTGGGGTGCACGCCAGGGGTAACACGGGTGATGCTGGTGTCATTGATGCTCAACTGTTCCAGGCGCGCC‑CGGCCCCCGaggcgcgcctggaacagttga                                                                                                                              >  6:272709‑M2/1‑116 (MQ=255)
                                 cTGGTCTTGTGGCACGCCGATGGCCACCCGGGCCTTGGGGTGCACGCCAGGGGTAACACGGGTGATGCTGGTGTCATTGATGCTCAACTGTTCCAGGCGCGCCTCGGGGGCCGGGCGCGTCCACAGCCAGAAGGCCAGCGCACCACCCa                                                                                                               <  5:236318/149‑1 (MQ=255)
                                       ttGTGGCACGCCGATGGCCACCCGGGCCTTGGCGTGCACGCCAGGGGTAACACGGGTGATGCTGGTGTCATTGATGCTCAACTGTTCCAGGCGCGCCTCGGGGGCCGGGCGCTTCAACAGCGAGACGGCCCGCCCCCCACCCCGggcgg                                                                                                         >  8:233562/1‑149 (MQ=255)
                                         gtgGCACGCCGATGGCCACCCGGGCCTTGGGGTGCACGCCAGGGGTAACACGGGTGATGCTGGTGTCATTGATGCTCAACTGTTCCAGGCGCGCCTCGGGGGCCGGGCGCGTCCACAGCCAGAAGGCCa                                                                                                                           <  8:202928/129‑1 (MQ=255)
                                         gtgGCACGCCGATGGCCACCCGGGCCTTGGGGTGCACGCCAGGGGTAACACGGGTGATGCTGGTGTCATTGATGCTCAACTGTTCCAGGCGCGCCTCGGGGGCCGGGCGCGTCCACAGCCAGAAGGCCa                                                                                                                           >  7:202928/1‑129 (MQ=255)
                                            gCACGCCGATGGCCACCCGGGCCTTGGGGTGCACGCCAGGGGTAACACGGGTGATGCTGGTGTCATTGATGCTCAACTGTTCCAGGCGCGCCTCGGGGGCCGGGCGCGTCCACAGCCAGAAGGCCAGCGCACCACCCAGGGCGGCcag                                                                                                     <  7:53286/148‑1 (MQ=255)
                                              aCGCCGATGGCCACCCGGGCCTTGGGGTGCACGCCAGGGGTAACACGGGTGATGCTGGTGTCATTGATGCTCAACTGTTCCAGGCGCGCCTCGGGGGCCGGGCGCGTCCACAGCCAGAAGGCCAGCGCACCACCCAGGGCGGCcagcag                                                                                                  >  1:134351/1‑149 (MQ=255)
                                                    aTGGCCACCCGGGCCTTGGGGTGCACGCCAGGGGTAACACGGGTGATGCTGGTGTCATTGATGCTCAACTGTTCCAGGCGCGCCTCGGGGGCCGGGCGCGTCCACAGCCAGAAGGCCAGCGCACCACCCAGGGCGGCCAGCAGCAgg                                                                                              <  7:194450/147‑1 (MQ=255)
                                                    aTGGCCACCCGGGCCTTGGGGTGCACGCCAGGGGTAACACGGGTGATGCTGGTGTCATTGATGCTCAACTGTTCCAGGCGCGCCTCGGGGGCCGGGCGCGTCCACAGCCAGAAGGCCAGCGCACCACCCAGGGCGGCCAGCAGCAgg                                                                                              >  8:194450/1‑147 (MQ=255)
                                                       gCCACCCGGGCCTTGGGGTGCACGCCAGGGGTAACACGGGTGATGCTGGTGTCATTGATGCTCAACTGTTCCAGGCGCGCCTCGGGGGCCGGGCGCGTCCACAGCCAGAAGGCCAGCGCACCACCCAGGGCGGCCAGCAGCAGGGGAAc                                                                                         <  2:63278/149‑1 (MQ=255)
                                                        ccACCCGGGCCTTGGGGTGCACGCCAGGGGTAACACGGGTGATGCTGGTGTCATTGATGCTCAACTGTTCCAGGCGCGCCTCGGGGGCCGGGCGCGTCCACAGCCAGAAGGCCAGCGCACCACCCAGGGCGGCCAGCAGCAGGGGAAc                                                                                         >  6:23452/1‑148 (MQ=255)
                                                        ccACCCGGGCCTTGGGGTGCACGCCAGGGGTAACACGGGTGATGCTGGTGTCATTGATGCTCAACTGTTCCAGGCGCGCCTCGGGGGCCGGGCGCGTCCACAGCCAGAAGGCAAGCGCACCACCCAGGGCGGCCAGCAGCAGGGGAAc                                                                                         >  7:121566/1‑148 (MQ=255)
                                                            ccGGGCCTTGGGGTGCACGCCAGGGGTAACACGGGTGATGCTGGTGTCATTGATGCTCAACTGTTCCAGGCGCGCCTCGGGGGCCGg                                                                                                                                                  <  1:154477‑M2/87‑2 (MQ=255)
                                                            ccGGGCCTTGGGGTGCACGCCAGGGGTAACACGGGTGATGCTGGTGTCATTGATGCTCAACTGTTCCAGGCGCGCCTCGGGGGCCGg                                                                                                                                                  >  2:154477‑M2/1‑86 (MQ=255)
                                                            ccGGGCCTTGGGGTGCACGCCAGGGGTAACACGGGTGATGCTGGTGTCATTGATGCTCAACTGTTCCAGGCGCGCCTCGGGGGCCGGGCGCGTCCACAGCCAGAAGGCCAGCGCACCACCCAGGGCGGCCAGCAGCAGGGGAACcagca                                                                                    <  4:202123/149‑1 (MQ=255)
                                                            ccGGGCCTTGGGGTGCACGCCAGGGGTAACACGGGTGATGCTGGTGTCATTGATGCTCAACTGTTCCAGGCGCGCCTCGGGGGCCGGGCGCGTCCACAGCCAGAAGGCCAGCGCACCACCCAGGGCGGCCAGCAGCAGGGGAACcagca                                                                                    <  5:23452/149‑1 (MQ=255)
                                                                        gTGCACGCCAGGGGTAACACGGGTGATGCTGGTGTCATTGATGCTCAACTGTTCCAGGCGCGCCTCGGGGGCCGGGCGCGTCCACAGCCAGAAGGCCAGCGCACCACCCAGGGCGGCCAGCAGCAGGGGAACCAGCAGGTACAACaaa                                                                         >  6:126511/1‑148 (MQ=255)
                                                                         tGCACGCCAGGGGTAACACGGGTGATGCTGGTGTCATTGATGCTCAACTGTTCCAGGCGCGCCTCGGGGGCCGGGCGCGTCCACAGCCAGAAGGCCAGCGcac                                                                                                                     <  8:121295/103‑1 (MQ=255)
                                                                         tGCACGCCAGGGGTAACACGGGTGATGCTGGTGTCATTGATGCTCAACTGTTCCAGGCGCGCCTCGGGGGCCGGGCGCGTCCACAGCCAGAAGGCCAGCGcac                                                                                                                     >  7:121295/1‑103 (MQ=255)
                                                                           cACGCCAGGGGTAACACGGGTGATGCTGGTGTCATTGATGCTCGACTGTTCCAGGCGCGCC‑CGGCCCCCGaggcgcgcctggaac                                                                                                                                    <  2:196915‑M2/85‑16 (MQ=255)
                                                                           cACGCCAGGGGTAACACGGGTGATGCTGGTGTCATTGATGCTCGACTGTTCCAGGCGCGCC‑CGGCCCCCGaggcgcgcctggaac                                                                                                                                    >  1:196915‑M2/1‑70 (MQ=255)
                                                                               ccAGGGGTAACACGGGTGATGCTGGTGTCATTGATGCTCAACTGTTCCAGGCGCGCCTCGGGGGCCGGGCGCGTCCACAGCCAGAAGGCCAGCGCACCACCCAGGGCGGCCAGCAGCAGGGGAACCAGCAGGTACAACAAAAAGCGTCg                                                                 <  5:126511/149‑1 (MQ=255)
                                                                                cAGGGGTAACACGGGTGATGCTGGTGTCATTGATGCTCAACTGTTCCAGGCGCGCCTCGGGGGCCGGGCGCGTCCACAGCCAGAAGGCCAGCGCACCACCCAGGGCGGCCAGCAGCAgggg                                                                                            >  2:181587/1‑121 (MQ=255)
                                                                                cAGGGGTAACACGGGTGATGCTGGTGTCATTGATGCTCAACTGTTCCAGGCGCGCCTCGGGGGCCGGGCGCGTCCACAGCCAGAAGGCCAGCGCACCACCCAGGGCGGCCAGCAGCAgggg                                                                                            <  1:181587/121‑1 (MQ=255)
                                                                                         cacGGGTGATGCTGGTGTCATTGATGCTCAACTGTTCCAGGCGCGCCTCGGGGGCCGGGCGCGTCCACAGCCAGAAGGCCAGCGCACCACCCAGGGCGGCCAGCAGCAGGGGAACCAGCAGGTACAACAAAAAGCGTCGGGTCATCAGc                                                       >  1:204032/1‑149 (MQ=255)
                                                                                               tGATGCTGGTGTCATTGATGCTCAACTGTTCCAGGCGCGCCTCGGGGGCCGGGCGCGTCCACAGCCAGAAGGCCAGCGCACCACCCAGGGCGGCCAGCAGCAGGGGAACCAGCAGGTACAACAAAAAGCGTCGGGTCATCAGCGTTTCa                                                 <  2:134351/149‑1 (MQ=255)
                                                                                                 aTGCTGGTGTCATTGATGCTCAACTGTTCCAGGCGCGCCTCGGGGGCCGGGCGCGTCCACAGCCAGAAGGCCAGCGCACCACCCAGGGCGGCCAGCAGCAGGGGAACCAGCAGGTACAACAAAAAGCGTCGGGTCATCAGCGTTTCAc                                                <  8:121566/148‑1 (MQ=255)
                                                                                                     tGGTGTCATTGATGCTCAACTGTTCCAGGCGCGCCTCGGGGGCCGGGCGCGTCCACAGCCAGAAGGCCAGCGCACCACCCAGGGCGGCCAGCAGCAGGGGAACCAGCAGGTACAACAAAAAGCGTCGGGTCATCAGCGTTTCACCAATc                                           >  5:255555/1‑149 (MQ=255)
                                                                                                         gtCATTGATGCTCAACTGTTCCAGGCGCGCCTCGGGGGCCGGGCGCGTCCACAGCCAGAAGGCCAGCGCACCACCCAGGGCGGCCAGCAGCAGGGGAACCAGCAGGTACAACAAAAAGCGTCGGGTCATCAGCGTTTCACCAATCCAGt                                       >  3:130846/1‑149 (MQ=255)
                                                                                                            aTTGATGCTCAACTGTTCCAGGCTCGCCTCGGGGGCCGGGCGCGGACACATCCAGAATGCCAGCGCACCACCCAGGGCGGCCAGCAGCAGGGTAACCAGCAGGTACAACAAAAAGCGTCGGGTCATCAGCGTTTCACCAATCCAGTCAg                                    <  7:233562/149‑1 (MQ=255)
                                                                                                            aTTGATGCTCAACTGTTCCAGGCGCGCCTCGGGGGCCGGGCGCGTCCACAGCCAGAAGGCCAGCGCACCACCCAGggcg                                                                                                          >  8:247336/1‑79 (MQ=255)
                                                                                                            aTTGATGCTCAACTGTTCCAGGCGCGCCTCGGGGGCCGGGCGCGTCCACAGCCAGAAGGCCAGCGCACCACCCAGggcg                                                                                                          <  7:247336/79‑1 (MQ=255)
                                                                                                                   cTCAACTGTTCCAGGCGCGCCTCGGGGGCCGGGCGCGTCCACAGCCATAAGGCCAGCGCACCACCCAGGGCGGCCAGCAGCAGGGTAACCAGCAGGTACAACAAAAAGCGTCGGGTCATCAGCGTTTCACCAATCCAGTCAGGCCGCCt                             <  2:260404/149‑1 (MQ=255)
                                                                                                                   cTCAACTGTTCCAGGCGCGCCTCGGGGGCCGGGCGCGTCCACAGCCAGAAGGCCAGCGCACCACCCAGGGCGGCCAGCAGCAGGGGAACCAGCAGGTACAACAAAAAGCGTCGGGTCATCAGCGTTTCACCAATCCAGTCAGGCCGCCt                             >  6:232993/1‑149 (MQ=255)
                                                                                                                      aaCTGTTCCAGGCGCGCCTCGGGGGCCGGGCGCGTCCACAGTCAGAAGGCCAGCGCACCACCCAGGGCGGCCAGCAGTAGGGGAACCAGCAGGTACAACAAAAAGCGTCGGGTCATCAGCGTTTCACCAATCCAGTCAGGCCGCCTGc                           >  8:188243/1‑148 (MQ=255)
                                                                                                                           ttCCAGGCGCGCCTCGGGGGCCGGGCGCGTCCACAGCCAGAAGGCCAGCGCACCACCCAGGGCGGCCAGCAGCAGGGGAACCAGCAGGTACAACAAAAAGCGTCGGGTCATCAGCGTTTCACCAATCCAGTCAGGCCGCCTGCAATCa                      <  8:251613/148‑1 (MQ=255)
                                                                                                                            tCCAGGCGCGCCTCGGGGGCCGGGCGCGTCCACAGCCAGAAGGCCAGCGCACCACCCAGGGCGGCCAGCAGCAGGGGAACCAGCAGGTACAACAAAAAGCGTCGGGTCATCAGCGTTTCACCAATCCAGTCAGGCCGCCTGCAATCAgg                    >  8:151758/1‑149 (MQ=255)
                                                                                                                             ccAGGCGCGCCTCGGGGGCCGGGCGCGTCCACAGCCAGAAGGCCAGCGCACCACCCAGGACGGCCAGCAGCAGGGGAACCAGCAGGTACAACAAAAAGCGTCGGGTCATCAGCGTTTCACCAATCCAGTCAGGCCGCCt                             <  4:237151/139‑1 (MQ=255)
                                                                                                                             ccAGGCGCGCCTCGGGGGCCGGGCGCGTCCACAGCCAGAAGGCCAGCGCACCACCCAGGACGGCCAGCAGCAGGGGAACCAGCAGGTACAACAAAAAGCGTCGGGTCATCAGCGTTTCACCAATCCAGTCAGGCCGCCt                             >  3:237151/1‑139 (MQ=255)
                                                                                                                                 gcgcgcCTCGGGGGCCGGGCGCGTCCACAGCCAGAAGGCCAGCGCACCACCCAGGGCGGCCAGCAGCAGGGGAACCAGCAGGTACAACAAAAAGCGTCGGGTCATCAGCGTTTCACCAATCCAGTCAGGCCGCCTGCAATCAGggcggc               <  4:130846/149‑1 (MQ=255)
                                                                                                                                  cgcgcCTCGGGGGCCGGGCGCGTCCACAGCCAGAAGGCCAGCGCACCACCCAGGGCGGCCAGCAGCAGGGGAACCAGCAGGTACAACAAAAAGCGTCGGGTCATCAGCGTTTCACCAATCCAGTCAGGCCGCCTGCAATCAGGgcggcg              <  2:145785/149‑1 (MQ=255)
                                                                                                                                     gcCTCGGGGGCCGGGCGCGTCCACAGCCAGAAGGCCAGCGCACCACCCAGGGCGGCCAGCAGCAGGGGAACCAGCAGGTACAACAAAAAGCGTCGGGTCATCAGCGTTTCACCAATCCAGTCAGGCCGCCTGCAATCAGGGCGGCGgtg           >  3:122592/1‑149 (MQ=255)
                                                                                                                                     gcCTCGGGGGCCGGGCGCGTCCACAGCCAGAAGGCCAGCGCACCACCCAGGGCGGCCAGCAGCAGGGGAACCAGCAGGTACAACAAAAAGCGTCGGGTCATCAGCGTTTCACCAATCCAGTCAGGCCGCCTGCAATCAGGGCGGCGgtg           <  6:255555/149‑1 (MQ=255)
                                                                                                                                       cTCGGGGGCCGGGCGCGTCCACAGTCAGAAGGCCAGCGCACCACCCAGGGCGGCCAGCAGTAGGGGAACCAGCAGGTACAACAAAAAGCGTCGGGTCATCAGCGTTTCACCAATCCAGTCAGGCCGCCTGCAATCAGGGCGGCGGTGTc         <  7:188243/149‑1 (MQ=255)
                                                                                                                                       cTCGGGGGCCGGGCGCGTCCACAGCCAGAAGGCCAGCGCACCACCCAGGGCGGCCAGCAGCAGGGGAACCAGCAGGTACAACAAAAAGCGTCGGGTCATCAGCGTTTCACCAATCCAGTCAGGCCGCCTGCAATCAGGGCGGCGGTGTc         >  7:127094/1‑149 (MQ=255)
                                                                                                                                        tCGGGGGCCGGGCGCGTCCACAGCCAGAAGGCCAGCGCACCACCCAGGGCGGCCAGCAGCAGGGGAACCAGCAGGTACAACAAAAAGCGTCGGGTCATCAGCGTTTCACCAATCCAGTCAGGCCGCCTGCAATCAGGGCGGCGGTGTCg        >  6:54446/1‑149 (MQ=255)
                                                                                                                                              gCCGGGCGCGTCCACAGCCAGAAGGCCAGCGCACCACCCAGGGCGGCCAGCAGCAGGGGAACCAGCAGGTACAACAAAAAGCGTCGGGTCATCAGCGTTTCACCAATCCAGTCAGGCCGCCTGCAATCAGGGCGGCGGTGTCGGCCAGt  <  5:54446/149‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                                    
GCTCAGGTCGAGCAGTTGCTTGCCCGTCAGTGCCTGGTCTTGTGGCACGCCGATGGCCACCCGGGCCTTGGGGTGCACGCCAGGGGTAACACGGGTGATGCTGGTGTCATTGATGCTCAACTGTTCCAGGCGCGCCTCGGGGGCCGGGCGCGTCCACAGCCAGAAGGCCAGCGCACCACCCAGGGCGGCCAGCAGCAGGGGAACCAGCAGGTACAACAAAAAGCGTCGGGTCATCAGCGTTTCACCAATCCAGTCAGGCCGCCTGCAATCAGGGCGGCGGTGTCGGCCAGT  >  NC_002947/1377399‑1377689

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑