Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,382,539 A→C 7.5% D254E (GAT→GAG)  PP_1204 ← lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,382,5390AC 7.5% 156.9 / 2.4 51D254E (GAT→GAG) PP_1204lipoprotein
Reads supporting (aligned to +/- strand):  ref base A (23/24);  new base C (2/2);  total (25/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACTGCAGGAACTCCTGCTGCAGCTCCGGATCGTTGCGGGTCAGCTCGATCAGGCTCTGTTCCATTTCACTGGCTTCCTCTTCAAGGCCCAGTTCGGA‑CAGGCGCTTGACCCGGTGCACCCACTGGCCGACATCATCATCTTCGAGGTCATCGAAGATCAGCTCATGGGCTTCGAGCAGCTTATTGCGCAGGGTGGCGCTGACCAACAGGCTGGCATCGCCTCGTACCGCATTGTCCTCGTCAAGTACTTGCAGGTGCAGGGTGCCGACGTGGTTGAGGTTCTGCTCGGAGAACGGGC  >  NC_002947/1382391‑1382687
                                                                                                                                                     |                                                                                                                                                    
aCTGCAGGAACTCCTGCTGCAGCTCCGGATCGTTGCGGGTCAGCTCGATCAGGCTCTGTTCCATTTCACTGGCTTCCTCTTCAAGGCCCAGTTCGGA‑CAGGCGCTTGACCCGGTGCACCCACTGGCCGACATCATCATCTTCGAGGTCa                                                                                                                                                      >  1:131404/1‑149 (MQ=255)
    cAGGAACTCCTGCTGCAGCTCCGGATCGTTGCGGGTCAGCTCGATCAGGCTCTGTTCCATTTCACTGGCTTCCTCTTCAAGGCCCAGTTCGGA‑CAGGCGCTTGACCCGGTGCACCCACTGGCCGACATCATCATCTTCGAGGTCATc                                                                                                                                                    <  4:243592/147‑1 (MQ=255)
    cAGGAACTCCTGCTGCAGCTCCGGATCGTTGCGGGTCAGCTCGATCAGGCTCTGTTCCATTTCACTGGCTTCCTCTTCAAGGCCCAGTTCGGA‑CAGGCGCTTGACCCGGTGCACCCACTGGCCGACATCATCATCTTCGAGGTCATCg                                                                                                                                                   >  7:208336/1‑148 (MQ=255)
     aGGAACTCCTGCTGCAGCTCCGGATCGTTGCGGGTCAGCTCGATCTGGCTCTGTTCCATTTCACTGGCTTCCTCTTCAAGGCCCAGTTCGGA‑CAGGCGCTTGACCCGGTGCACCCACTGGCCGACATCATCATCTTCGAGGTCATCg                                                                                                                                                   >  1:189319/1‑147 (MQ=255)
       gAACTCCTGCGGCAGCTCCGGATCGTTGCGGGTCAGCTCGATCAGGCTCTGTTCCATTTCACTGGCTTCCTCTTCAAGGCCCAGTTCGGA‑CAGGCGCTTGACCCGGTGCACCCACTGGCCGACATCATCATCTTCGAGGTCATCg                                                                                                                                                   >  2:25630/1‑145 (MQ=255)
         aCTCCTGCTGCAGCTCCGGATCGTTGCGGGTCAGCTCGATCAGGCTCTGTTCCATTTCACTGGCTTCCTCTTCAAGGCCCAGTTCGGA‑CAGGCGCTTGACCCGGTGCACCCACTGGCCGACATCATCATCTTCGATGACCTCGAAGAtg                                                                                                                                             <  2:32048/149‑2 (MQ=255)
         aCTCCTGCTGCAGCTCCGGATCGTTGCGGGTCAGCTCGATCAGGCTCTGTTCCATTTCACTGGCTTCCTCTTCAAGGCCCAGTTCGGA‑CAGGCGCTTGACCCGGTGCACCCACTGGCCGACATCATCATCTTCGAGGTCATCGAAGATc                                                                                                                                             <  3:129131/149‑1 (MQ=255)
         aCTCCTGATGCAGCTCCGGATCGTTGCGGGTCAGCTCGATGAGGCTCTGTTCCATTTCACTGGCTTCCTCTTCAAGGCCCAGTTCGGA‑CAGGCGCTTGACCCGGTGCACCCACTGGCCGACATCATCATCTTCGATGACCTCGAAGAtg                                                                                                                                             <  8:162923/149‑2 (MQ=255)
                ctgcAGCTCCGGATCGTTGCGGGTCAGCTCGATCAGGCTCTGTTCCATTTCACTGGCTTCCTCTTCAAGGCCCAGTTCGGA‑CAGGCGCTTGACCCGGTGCACCCACTGGCCGACATCATCATCTTCGAGGTCATCGAAGATCAGCTCAt                                                                                                                                      <  8:208336/149‑1 (MQ=255)
                      cTCCGGATCGTTGCGGGTCAGCGCGATCAGGCTCTGTTCCATTTCACTGGCTTACTCTTCAAGGCCCAGTTCGGA‑CAGGCGCGTGACCCGGTGCACCCACTGGCCGACATCATCATCTTCGAGGTCATCGAAGATCAGCTCATGGGCtt                                                                                                                                <  7:250468/149‑1 (MQ=255)
                          ggATCGTTGCGGGTCAGCTCGATCAGGCTCTGTTCCATTTCACTGGCTTCCTCTTCAAGGCCCAGTTCGGA‑CAGGCGCTTGACCCGGTGCACCCACTGGCCGACATCATCATCTTCGAGGTCATCGAAGATCAGCTCATGGGCTTCGag                                                                                                                            <  2:131404/149‑1 (MQ=255)
                               gTTGCGGGTCAGCTCGATCAGGCTCTGTTCCATTTCACTGGCTTCCTCTTCAAGGCCCAGTTCGGA‑CAGGCGCTTGACCCGGTGCACCCACTGGCCGACATCATCATCTTCGAGGTCATCGAAGATCAGCTCATGGGCTTCGagcagc                                                                                                                        <  1:45281/148‑1 (MQ=255)
                                 tGCGGGTCAGCTCGATCAGGCTCTGTTCCATTTCACTGGCTTCCTCTTCAAGGCCCAGTTCGGA‑CAGGCGCTTGACCCGGTGCACCCACTGGCCGACATCATCATCTTCGAGGTCATCGAAGATCAGCTCATGGGCTTCGAGCAGCTTa                                                                                                                     >  4:207675/1‑149 (MQ=255)
                                     gggcAGCTCGATCAGGCTCTGTTCCATTTCACTGGCTTCCTCTTCAAGGCCCAGTTCGGA‑CAGGCGCTTGACCCGGTGCACCCACTGGCCGACATCATCATCTTCGAGGTCATCGAAGATCAGCTCATGGGCTTCGAGCAGCTTATTgc                                                                                                                 <  8:222121/146‑1 (MQ=255)
                                            tCGATCAGGCTCTGTTCCATTTCACTGGCTTCCTCTTCAAGGCCCAGTTCGGA‑CAGGCGCTTGACCCGGTGCACCCACTGGCCGACATCATCATCTTCGAGGTCATCGAAGATCAGCTCATGGGCTTCGAGCAGCTTATTGCGCAGGGt                                                                                                          >  5:97226/1‑149 (MQ=255)
                                                          ttCCATTTCACTGGCTTCCTCTTCAAGGCCCAGTTCGGA‑CAGGCGCTTGACCCGGTACACCCACTGGCAGACATCATCATCTTCGAGGTCATAGAAGATCAGCTCATGGGATTAGAGCAGCTTATTGCGCAGGGTCGCTCTTACCaa                                                                                              >  6:220355/1‑147 (MQ=255)
                                                              aTTTCACTGGCTTCCTCTTCAAGGCCCAGTTCGGA‑CAGGCGCTTGACCCGGTGCACCCACTGGCCGACATCATCATCTTCGAGGTCATCGAAGATCAGCTCATGGGCTTCGAGCAGCTTATTGCGCAGGGTGGCGCTGACCAACAGGCt                                                                                        >  4:224139/1‑149 (MQ=255)
                                                              aTTTCACTGGCTTCCTCTTCAAGGCCCAGTTCGGA‑CAGGCGCTTGACCCGGTGCACCCACTGGCCGACATCATCATCTTCGAGGTCATCGAAGATCAGCTCATGGGCTTCGAGCAGCTTATTGCGCAGGGTGGCGCTGACCAACAGGCt                                                                                        <  1:25630/149‑1 (MQ=255)
                                                               tttCACTGGCTTCCTCTTCAAGGCCCAGTTCGGAGCAGGCGCTTGACCCGGTGCACCCACTGGCCGACATCATCATCTTCGAGGTCATCGAAGATCAGCTCATGGGCTTCGAGCAGCTTATTGCGCAGGGTGGCGCTGACCAACAGGCt                                                                                        >  1:36519/1‑149 (MQ=255)
                                                               tttCACTGGCTTCCTCTTCAAGGCCCAGTTCGGA‑CAGGCGCTTGACCCGGTGCACCCACTGGCCGACATCATCATCTTCGAGGTCATCGAAGATCAGCTCATGGGCTTCGAGCAGCTTATTGCGCAGGGTGGCGCTGACCAACAGGCTg                                                                                       <  7:84621/149‑1 (MQ=255)
                                                                ttCACTGGCTTCCTCTTCAAGGCCCAGTTCGGA‑CAGGCGCTTGACCCGGTGCACCCACTGGCCGACATCATCATCTTCGAGGTCATCGAAGATCAGCTCATGGGCTTCGAGCAGCTTATTGCGCAGGGTGGCGCTGACCAACAGGCTgg                                                                                      >  4:146653/1‑149 (MQ=255)
                                                                   aCTGGCTTCCTCTTCAAGGCCCAGTTCGGA‑CAGGCGCTTGACCCGGTGCACCCACTGGCCGACATCATCATCTTCGAGGTCATCGAAGATCAGCTCATGGGCTTCGagcag                                                                                                                         <  2:236991/111‑1 (MQ=255)
                                                                   aCTGGCTTCCTCTTCAAGGCCCAGTTCGGA‑CAGGCGCTTGACCCGGTGCACCCACTGGCCGACATCATCATCTTCGAGGTCATCGAAGATCAGCTCATGGGCTTCGagcag                                                                                                                         >  1:236991/1‑111 (MQ=255)
                                                                   aCTGGCTTCCTCTTCAAGGCCCAGTTCGGA‑CAGGCGCTTGACCCGGTGCACCCACTGGCCGACATCATCATCTTCGAGGTCATCGAAGATCAGCTCATGGGCTTCGAGCAGCTTATTGCGCAGGGTGGCGCTGACCAACAGGCTGGCAt                                                                                   >  2:268773/1‑149 (MQ=255)
                                                                         ttCCTCTTCAAGGCCCAGTTCGGA‑CAGGCGCTTGACCCGGTGCACCCACTGGCCGACATCATCATCTTCGAGGTCATCGAAGATCAGCTCATGGGGTTCGAGCAGCTTATTGCGCAGGGTGGCGCTGACCAACAGGCTg                                                                                       >  1:219834/1‑139 (MQ=255)
                                                                         ttCCTCTTCAAGGCCCAGTTCGGA‑CAGGCGCTTGACCCGGTGCACCCACTGGCCGACATCATCATCTTCGAGGTCATCGAAGATCAGCTCATGGGCTTCGAGCAGCTTATTGCGCAGGGTGGCGCTGACCAACAGGCTg                                                                                       <  2:219834/139‑1 (MQ=255)
                                                                            ctctTCAAGGCCCAGTTCGGA‑CATGCGCTTGACCCGGTGCACCCACTGGCCGACATCATCATCTTCGAGGTCATCGAAGATCAGCTCATGGGCTTCGAGCAGCTTATTGCGCAGGGTGGCGCTGACCAACAGGCTGGCATCGCCTCGt                                                                           <  7:191718/148‑1 (MQ=255)
                                                                                 cAAGGCCCAGTTCGGA‑CAGGCGCTTGACCCGGTGCACCCACTGGCCGACATCATCATCTTCGAGGTCATCGAAGATAAGCTCATGGGCTTCGAGCAGCTTATTGCGCAGGGTGGCGCTGACCAACAGGCTGGCATCGCCTCGTACCGCa                                                                     >  5:260591/1‑149 (MQ=255)
                                                                                          gTTCGGA‑CAGGCGCTTGACCCGGTGCACCCACTGGCCGACATCATCATCTTCGAGGTCATCGAAGATCAGCTCATGGGCTTCGAGCAGCTTATTGCGCAGGGTGGCGCTGACCAACAGGCTGGCATCGCCTCGTACCGCATTGTCCTCg                                                            >  5:115483/1‑149 (MQ=255)
                                                                                              ggA‑CAGGCGCTTGACCCGGTGCACCCACTGGCCGACATCATCATCTTCGAGGTCATCGAAGATCAGCTCATGGGCTTCGAGCAGCTTATTGCGCAGGGTGGCGCTGACCAACAGGCTGGCATCGCCTCGTACCGCATTGTCCTCGTCaa                                                        <  6:115483/149‑1 (MQ=255)
                                                                                               gA‑CAGGCGCTTGACCCGGCGCACCCACTGGCCGACATCATCATCTTCGAGGTCATCGAAGATCAGCTCATGGGCTTCGagca                                                                                                                          <  6:167454/82‑1 (MQ=255)
                                                                                               gA‑CAGGCGCTTGACCCGGCGCACCCACTGGCCGACATCATCATCTTCGAGGTCATCGAAGATCAGCTCATGGGCTTCGagca                                                                                                                          >  5:167454/1‑82 (MQ=255)
                                                                                                a‑CAGGCGCTTGACCCGGTGCACCCACTGGCCGACATCATCATCTTCGAGGTCATCGAAGATCAGCTCATGGGCTTCGAGCAGCTTATTGCGCAGGGTGGCGCTGACCAACAGGCTGGCATCGc                                                                                >  7:92691/1‑123 (MQ=255)
                                                                                                a‑CAGGCGCTTGACCCGGTGCACCCACTGGCCGACATCATCATCTTCGAGGTCATCGAAGATCAGCTCATGGGCTTCGAGCAGCTTATTGCGCAGGGTGGCGCTGACCAACAGGCTGGCATCGc                                                                                <  8:92691/123‑1 (MQ=255)
                                                                                                  cAGGCGCTTGACCCGGTGCACCCACTGGCCGACATCATCATCTTCGAGGTCATCGAAGATCAGCTCATGGGCTTCGAGCAGCTTATTGCGCAGGGTGGCGCTGACCAACAGGCTGGCATCGCCTCGTACCGCATTGTCCTCGTCAAGTa                                                     <  2:36519/149‑1 (MQ=255)
                                                                                                     gcgcTTGACCCGGTGCACCCACTGGCCGACATCATCATCTTCGAGGTCATCGAAGATCAGCTCATGGGCTTCGAGCAGCTTATTGCGCAGGGTGGCGCTGACCAACAGGCTGGCATCGCCTCGTACCGCATTGTCCTCGTCAAGTACtt                                                  >  5:114669/1‑149 (MQ=255)
                                                                                                              ccGGTGCACCCACTGGCCGACATCATCATCTTCGAGGTCATCGAAGATCAGCTCATGGGCTTCGAGCAGCTTATTGCGCAGGGTGGCGCTGACCAACAGGCTGGCATCGCCTCGTACCGCATTGTCCTCGTCAAGTACTtgcaggtgca                                         <  2:189319/149‑1 (MQ=255)
                                                                                                               ctgTGAACCCACTGGCCGACATCATAAACTACGAGGTCATCGAAGATCAGCTCATGGGCTTCGAGCAGCTTATTGCGCAGGCTGTCGCTGACAAACAGGCTGGCATCGCCTCGTACCGCATTGACCTCGTCAAGTAATtcaaggtcaac                                        >  6:153916/3‑145 (MQ=255)
                                                                                                                    cACCCACTGGCCGACATCATCATCTTCGAGGTCATCGAAGATCAGCTCATGGGCTTCGAGCAGCTTATTGCGCAGGGTGGCGCTGACCAACAGGCTGGCATCGCCTCGTACCGCATTGTCCTCGTCAAGTACTTGCAGGTGCAGGGTGc                                   <  3:207675/149‑1 (MQ=255)
                                                                                                                       ccACTGGCCGACATCATCATCTTCGAGGTCATCGAAGATCAGCTCATGGGCTTCGAGCAGCTTATTGCGCAGGGTGGCGCTGACCAACAGGCTg                                                                                       >  2:103891/1‑94 (MQ=255)
                                                                                                                       ccACTGGCCGACATCATCATCTTCGAGGTCATCGAAGATCAGCTCATGGGCTTCGAGCAGCTTATTGCGCAGGGTGGCGCTGACCAACAGGCTg                                                                                       <  1:103891/94‑1 (MQ=255)
                                                                                                                             gCCGACATCATCATCTTCGAGGTCATCGAAGATCAGCTCATGGGCTTCGAGCAGCTTATTGCGCAGGGTGGCGCTGACCAACAGGCTGGCATCGCCTCGTACCGCATTGTCCTCGTCAAGTACTTGCAGGTGCAGGGTGCCGACGTgg                           <  1:268773/148‑1 (MQ=255)
                                                                                                                                gACATCTTCATCTTCGAGGTCAACGAAGATCAGCTCATGTGCTTCGAGCAGCTTATTGCGCAGGGTGGCGCTGACCAACAGGCTGGCATCGCCTCGTACCGCATTGTCCTCGTCAAGTACTTGCAGGTGCAGGGTGCCGACGTGGTTGa                       <  5:220355/149‑1 (MQ=255)
                                                                                                                                  catcatcatCTTCGAGGTCATCGAAGATCAGCTCATGGGCTTCGAGCAGCTTATTGCGCAGGGTGGCGCTGACCAACAGGCTGGCATCGCCTCGTACCGCATTGTCCTCGTCAAGTACTTGCAGGTGCAGGGTGCCGACGTGGTTGAgg                     <  6:114669/149‑1 (MQ=255)
                                                                                                                                      atcatcTTCGAGGTCATCGAAGATCAGCTCATGGGCTTCGAGCAGCTTATTGCGCAGGGTGGCGCTGACCAACAGGCTGGCATCGCCTCGTACCGCATTGTCCTCGTCAAGTACTTGCAGGTGCAGGGTGCCGACGTGGtt                         <  4:74233/141‑1 (MQ=255)
                                                                                                                                      atcatcTTCGAGGTCATCGAAGATCAGCTCATGGGCTTCGAGCAGCTTATTGCGCAGGGTGGCGCTGACCAACAGGCTGGCATCGCCTCGTACCGCATTGTCCTCGTCAAGTACTTGCAGGTGCAGGGTGCCGACGTGGtt                         >  3:74233/1‑141 (MQ=255)
                                                                                                                                      atcatcTTCGAGGTCATCGAAGATCAGCTCATGGGCTTCGAGCAGCTTATTGCGCAGGGTGGCGCTGACCAACAGGCTGGCATCGCCTCGTACCGCATTGTCCTCGTCAAGTACTTGCAGGTGCAGGGTGCCGACGTGGTTGAGGTTCt                 >  7:173838/1‑149 (MQ=255)
                                                                                                                                         atcTTCGATGACCTCGAAGATCAGCTCATGGGCTTCGAGCAGCTTATTGCGCAGGGTGGCGCTGACCAACAGGCTGGCATCGCCTCGTACCGCATTGTCCTCGTCAAGTACTTGCAGGTGCAGGGTGCCGACGTGGTTGAGGTTCTGCt              >  2:53793/1‑149 (MQ=255)
                                                                                                                                         atcTTCGATGACCTCGAAGATCAGCTCATGGGCTTCGAGCAGCTTATTGCGCAGGGTGGCGCTGACCAACAGGCTGGCATCGCCTCGTACCGCATTGTCCTCGTCAAGTACTTGCAGGTGCAGGGTGCCGACGTGGTTGAGGTTCTGCt              >  4:151569/1‑149 (MQ=255)
                                                                                                                                         atcTTCGAGGTCATCGAAGATCAGCTCATGGGCTTCGAGCAGCTTATTGCGCAGGGTGGCGCTGACCAACAGGCTGGCATCGCCTCGTACCGCATTGTCCTCGTCAAGTACTTGCAGGTGCAGGGTGCCGACGTGGTTGAGGTTCTGCt              >  4:226957/1‑149 (MQ=255)
                                                                                                                                                  gTCATCGAAGATCAGCTCATGGGCTTCGAGCAGCTTATTGCGCAGGGTGGCGCTGACCAACAGGCTGGCATCGCCTCGTACCGCATTGTCCTCGt                                                           >  2:4762/1‑95 (MQ=255)
                                                                                                                                                  gTCATCGAAGATCAGCTCATGGGCTTCGAGCAGCTTATTGCGCAGGGTGGCGCTGACCAACAGGCTGGCATCGCCTCGTACCGCATTGTCCTCGt                                                           <  1:4762/95‑1 (MQ=255)
                                                                                                                                                     aTCGAAGATCAGCTCATGGGCTTCGAGCAGCTTATTGCGCAGGGTGGCGCTGACCAACAGGCTGGCATCGCGTCGTACCGCATTGTCCTCGTCAAGTACTTGCAGGTGCAGGGTGCCGACGGGGTTGAGGTTCTGCTCGGAGAACGGGc  >  6:69330/1‑149 (MQ=255)
                                                                                                                                                     aTCGAAGATCAGCTCATGGGCTTCGAGCAGCTTATTGCGCAGGGTGGCGCTGACCAACAGGCTGGCATCGCCTCGTACCGCATTGTCCTCGTCAAGTACTTGCAGGTGCAGGGTGCCGACGTGGTTGAGGTTCTGCTCGGAGAACggg   <  5:153916/148‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
ACTGCAGGAACTCCTGCTGCAGCTCCGGATCGTTGCGGGTCAGCTCGATCAGGCTCTGTTCCATTTCACTGGCTTCCTCTTCAAGGCCCAGTTCGGA‑CAGGCGCTTGACCCGGTGCACCCACTGGCCGACATCATCATCTTCGAGGTCATCGAAGATCAGCTCATGGGCTTCGAGCAGCTTATTGCGCAGGGTGGCGCTGACCAACAGGCTGGCATCGCCTCGTACCGCATTGTCCTCGTCAAGTACTTGCAGGTGCAGGGTGCCGACGTGGTTGAGGTTCTGCTCGGAGAACGGGC  >  NC_002947/1382391‑1382687

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑