Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,382,939 C→T 8.1% G121D (GGT→GAT)  PP_1204 ← lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,382,9390CT 8.1% 138.3 / 2.5 49G121D (GGT→GAT) PP_1204lipoprotein
Reads supporting (aligned to +/- strand):  ref base C (22/23);  new base T (2/2);  total (24/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.09e-01

TTCCTCGCGCTTGCCTTTCTGCACTTCGCTGATCCCGGCCAGGTTCTGCTGGGCGCGGCCGTTGGATGGCACGTTCATGAACGGGTTGAGCCCATCCACGCCATAGCTGAGCCAGTCGTGGGTGATACTTTGCGGCAGGTTACCCAAGGCGAAGACGTTGACCACATTGGCGCCGACACCCGCCACCACGGCCACTGCACCCAGCGGGATCTCGTAGATCTCCCGCCAGGGTTGGTAAGGCGTGTAGCGGTCATAACGACGGGTCACTTCGAATTCGGTGACTTCGAA  >  NC_002947/1382797‑1383084
                                                                                                                                              |                                                                                                                                                 
ttCCTCGCGCTTGCCTTTCTGCACTTCGCTGATCCCGGCCAGCTTCTCCTGGGCGCGGCCGTTGGATGGCACGTTCATGAACGGGATGAGCCCAACCACCCCAAAGCTGAGCCAGTCGTGGGTGATACTTTGCGGCAGGTTACCCAAgg                                                                                                                                             >  3:82533/1‑149 (MQ=255)
    tCGCGCTTGCCTTTCTGCACTTCGCTGATCCCGGCCAGGTTCTGCTGGGCGCGGCCGTTGGATGGCACGTTCATGAACGGGTTGAGCCCATCCACGCCATAGCTGAGCCAGTCGTGGGTGATACTTTGCGGCAGGTTACCCAAGGCg                                                                                                                                           >  7:28520/1‑147 (MQ=255)
        gcTTGCCTTTCTGCTCTTCGCTGATCCCGGCCAGGTTCTGCTGGGCGCGGCCGTTGGATGGCACGTTCATGAACGGGTTGAGCCCATCCACGCCATAGCTGAGCCAGTCGTGGGTGATACTTTGCGGCAGGTTACCCAAGGCGAAGACg                                                                                                                                     <  7:55811/149‑1 (MQ=255)
        gcTTGCCTTTCTGCACTTCGCTGATCCCGGCCAGGTTCTGCTGGGCGCGGCCGTTGGATGGCACGTTCATGAACGGGTTGAGCCCATCCACGCCATAGCTGAGCCAGTCGTGGGTGATACTTTGCGGCAGGTTACCCAAGGCGAAGACg                                                                                                                                     <  1:72766/149‑1 (MQ=255)
         cTTGCCTTTCTGCACTTCGCTGATCCCGGCCAGGTTCTGCTGGGCGCGGCCGTTGGATGGCACGTTCATGAACGGGTTGAGCCCATCCACGCCATAGCTGAGCCAGTCGTGGGTGATACTTTGCGGCAGGTTACCCAAGGCGAAGACGt                                                                                                                                    <  6:31859/149‑1 (MQ=255)
                     cACTTCGCTGATCCCGGCCAGGTTCTGCTGNGCGCGGCCGTTGGATGGCACGTTCATGAACGGGTTGAGCCCATCCACGCCATAGCTGAGCCAGTCGTGGGTGATACTTTGCGGCAGGTTATCCAAGGCGAAGACGTTGACca                                                                                                                              >  1:22879/1‑143 (MQ=255)
                     cACTTCGCTGATCCCGGCCAGGTTCTGCTGGGCGCGGCCGTTGGATGGCACGTTCATGAACGGGTTGAGCCCATCCACGCCATAGCTGAGCCAGTCGTGGGTGATACTTTGCGGCAGGTTATCCAAGGCGAAGACGTTGACca                                                                                                                              <  2:18677/143‑1 (MQ=255)
                     cACTTCGCTGATCCCGGCCAGGTTCTGCTGGGCGCGGCCGTTGGATGGCACGTTCATGAACGGGTTGAGCCCATCCACGCCATAGCTGAGCCAGTCGTGGGTGATACTTTGCGGCAGGTTATCCAAGGCGAAGACGTTGACca                                                                                                                              <  2:22879/143‑1 (MQ=255)
                     cACTTCGCTGATCCCGGCCAGGTTCTGCTGGGCGCGGCCGTTGGATGGCACGTTCATGAACGGGTTGAGCCCATCCACGCCATAGCTGAGCCAGTCGTGGGTGATACTTTGCGGCAGGTTATCCAAGGCGAAGACGTTGACca                                                                                                                              >  1:18677/1‑143 (MQ=255)
                        ttCGCTGATCCCGGCCAGGTTCTGCTGGGCGCGGCCGTTGGATGGCACGTTCATGAACGGGTTGAGCCCATCCACGCCATAGCTGAGCCAGTCGTGGGTGATACTTTGCGGCAGGTTACCCAAGGCGAAGACGTTGACCACATTGgcgc                                                                                                                     >  7:61371/1‑149 (MQ=255)
                               aTCCCGGCCAGGTTCTGCTGGGCGCGGCCGTTGGATGGCACTTTCATGAACGGGTTGAGCCCATCCACGCCATAGCTGAGCCAGTCGTGGGTGATACTTTGCGGCAGGTTACCCAAGGCGAAGACGTTGACCACATTGGCGCCGaca                                                                                                                <  4:37324/147‑1 (MQ=255)
                                tCCCGGCCAGGTTCTGCTGGGCGCGGCCGTTGGATGGCACGTTCATGAACGGGTTGAGCCCATCCACGCCATAGCTGAGCCAGTCGTGGGTGATACTTTGCGGCAGGTTACCCAAGGCGAAGACGTTGACCACATTGGCGCCGACAccc                                                                                                             >  7:239613/1‑149 (MQ=255)
                                   cGGCCAGGTTCTGCTGGGCGCGGCCGTTGGATGGCACGTTCATGAACGGGTTGAGCCCATCCACGCCATAGCTGAGCCAGTCGTGGGTGATACTTTGCGGCAGGTTACCCAAGGCGAAGACGTTGACCACATTGGCGCCGACACCCGcc                                                                                                          >  4:27258/1‑149 (MQ=255)
                                               tctgGGTGCGGCCGTTGGATGGCTCGTTCATGTACGGGTTGAGCCCTTCGACGCCATCGCTGAGCCCGTCGGGGGTGATACTTTGCGGCAGGTTACCCAAGGCGAAGACGTTGACCACATTGGCGCCGAGACCCGCGTCCTCGGCCACt                                                                                              <  4:82533/148‑1 (MQ=255)
                                               gctgGGCGCGGCCGTTGGATGGCACGTTCATGAACGGGTTGAGCCCATCCACGCCATAGCTGAGCCAGTCGTGGGTGATACTTTGCGGCAGGTTACCCAAGGCGAAGACGTTGACCACATTGGCGCCGACACCCGCCACCACGGCCACt                                                                                              >  4:67816/1‑149 (MQ=255)
                                                 tgGGCGCGGCCGTTGGATGGCACGTTCATGAACGGGTTGAGCCCATCCACGCCATAGCTGAGCCAGTCGTGGGTGATACTTTGCGGCAGGTTACCCAAGGCGAAGACGTTGACCACATTGGCGCCGACACCCGCCACCACGGCCACTGc                                                                                            <  8:221319/149‑1 (MQ=255)
                                                        ggCCGTTGGATGGCACGTTCATGAACGGGTTGAGCCCATCCACGCCATAGCTGAGCCAGTCGTGGGTGATACTTTGCGGCAGGTTACCCAAGGCGAAGACGTTGACCACATTGGCGCCGACAccc                                                                                                             <  5:202285/125‑1 (MQ=255)
                                                        ggCCGTTGGATGGCACGTTCATGAACGGGTTGAGCCCATCCACGCCATAGCTGAGCCAGTCGTGGGTGATACTTTGCGGCAGGTTACCCAAGGCGAAGACGTTGACCACATTGGCGCCGACAccc                                                                                                             >  6:202285/1‑125 (MQ=255)
                                                            gTTGGATGGCACGTTCATGAACGGGTTGAGCCCATCCACGCCATAGCTGAGCCAGTCGTGGGTGATACTTTGCGGCAGGTTACCCAAGGCGAAGACGTTGACCACATTGGCGCCGACACCCGCCACCACGGCCACTGCACCCAGCGGGa                                                                                 >  3:194947/1‑149 (MQ=255)
                                                            gTTGGATGGCACGTTCATGAACGGGTTGAGCCCATCCACGCCATAGCTGAGCCAGTCGTGGGTGATACTTTGCGGCAGGTTACCCAAGGCGAAGACGTTGACCACATTGGCGCCGACACCCGCCACCACGGCCACTGCACCCAGCGGGa                                                                                 <  3:27258/149‑1 (MQ=255)
                                                               ggATGGCACGTTCATGAACGGGTTGAGCCCATCCACGCCATAGCTGAGCCAGTCGTGGGTGATACTTTGCGGCAGGTTACCCAAGGCGAAGACGt                                                                                                                                    >  2:225319/1‑95 (MQ=255)
                                                               ggATGGCACGTTCATGAACGGGTTGAGCCCATCCACGCCATAGCTGAGCCAGTCGTGGGTGATACTTTGCGGCAGGTTACCCAAGGCGAAGACGt                                                                                                                                    <  1:225319/95‑1 (MQ=255)
                                                                  tGGCACGTTCATGAACGGGTTGAGCCCATCCACGCCATAGCTGAGCCAGTCGTGGGTGATACTTTGCGGCAGGTTACCCAAGGCGAAGACGTTGACCACATTGGCGCCGACACCCGCCACCACGGCCACTGCACCCAGCGGGATCTCg                                                                            >  2:162474/1‑148 (MQ=255)
                                                                      aCGTTCATGAACGGGTTGAGCCCATCCACGCCATAGCTGAGCCAGTCGTGGGTGATACTTTGCGGCAGGTTACCCAAGGCGAAGACGTTGACCACATTGGCGCCGACACCCGCCACCACGGCCa                                                                                                >  3:253413/1‑124 (MQ=255)
                                                                      aCGTTCATGAACGGGTTGAGCCCATCCACGCCATAGCTGAGCCAGTCGTGGGTGATACTTTGCGGCAGGTTACCCAAGGCGAAGACGTTGACCACATTGGCGCCGACACCCGCCACCACGGCCa                                                                                                <  4:253413/124‑1 (MQ=255)
                                                                      aCGGTCATGAACGGGTTGAGCCCATCCACGCCATAGCTGAGCCAGTCGTGGGGGATACTTTGCGGCAGGGTACCCAAGGCGAAGACGTTGACCACATTGGCGCCGACACCCGCCACCACGGCCACTGCACCCAGCGGGATCTCGTa                                                                          <  5:171866/146‑1 (MQ=255)
                                                                        gTTCATGAACGGGTTGAGCCCATCCACGCCATAGCTGAGCCAGTCGTGGGTGATACTTTGCGGCAGGTTACCCAAGGCGAAGACGTTGACCACATTGGCGCCGACACCCGCCACCACGGCCACTGCACCCAGc                                                                                     >  1:131690/1‑133 (MQ=255)
                                                                        gTTCATGAACGGGTTGAGCCCATCCACGCCATAGCTGAGCCAGTCGTGGGTGATACTTTGCGGCAGGTTACCCAAGGCGAAGACGTTGACCACATTGGCGCCGACACCCGCCACCACGGCCACTGCACCCAGc                                                                                     <  2:131690/133‑1 (MQ=255)
                                                                                aCGGGTTGAGCCCATCCACGCCATAGCTCAGCCACTCGTGGGTGATACTTTGCGGCAGGTTACCCAAGGCGAAGACGTTGCCCACATTGGCGCCGACACCCGCCACCACGGCCACTGCACCCAGCGGGATCTCGt                                                                           >  7:100316/1‑135 (MQ=255)
                                                                                aCGGGTTGACGCGATGCACGGCATAGCTGAGCCAGTCGTGGGTGATACTTTGCGGCAGGTTACCCAAGGCGAAGACGTTGACCACATTGGCGCCGACACCCGCCACCACGGCCACTGCACCCAGCGGGATCTCGt                                                                           <  8:100316/135‑1 (MQ=255)
                                                                                 cGGGGTGAGCCCATCCACGCCATAGCTGAGCCAGTCGTGGGTGATACTTTGCGGCAGGTTACCCAAGGCGCAGACGTTGACCACATTGGCGCCGACACCCGCCACCACGGCCACTGCACCCAGCGGGATCTCGTAGATCTCCCGCCAgg                                                            <  8:239613/149‑1 (MQ=255)
                                                                                         gCCCATCCACGCCATAGCTGAGCCAGTCGTGGGTGATACTTTGCGGCAGGTTACCCAAGGCGAAGACGTTGACCACATTGGCGCCGACACCCGCCACCACGGCCACTGCACCCAGCGGGATCTCGTAGATCTCCCGCCAGGGTTGGTaa                                                    <  3:193613/149‑1 (MQ=255)
                                                                                            cATCCACGCCATAGCTGAGCCAGTCGTGGGTGATACTTTGCGGCAGGTTACCCAAGGCGAAGACGTTGACCACATTGGCGCCGACACCCGCCACCACGGc                                                                                                  >  5:122083/1‑100 (MQ=255)
                                                                                            cATCCACGCCATAGCTGAGCCAGTCGTGGGTGATACTTTGCGGCAGGTTACCCAAGGCGAAGACGTTGACCACATTGGCGCCGACACCCGCCACCACGGc                                                                                                  <  6:122083/100‑1 (MQ=255)
                                                                                            cATCCACGCCATAGCTGAGCCAGTCGTGGGTGATACTTTGCGGCAGGTTACCCAAGGCGAAGACGTTGACCACATTGGCGCCGACACCCGCCACCACGGCCACTGCACCCAGCGGGATCTCGTAGATCTCCCGCCAGGGTTGGTAAGGc                                                 >  1:114505/1‑149 (MQ=255)
                                                                                            cATCCACGCCATAGCTGAGCCAGTCGTGGGTGATACTTTGCGGCAGGTTACCCAAGGCGAAGACGTTGACCACATTGGCGCCGACACCCGCCACCACGGCCACTGCACCCAGCGGGATCTCGTAGATCTCCCGCCAGGGTTGGTAAGGc                                                 >  2:58524/1‑149 (MQ=255)
                                                                                                   gCCATAGCTGAGCCAGTCGTGGGTGATACTTTGCGGCAGGTTACCCAAGGCGAAGACGTTGACCACATTGGCGCCGACACCCGCCACCACGGCCACTGCACCCAGCGGGATCTCGTAGATCTCCCGCCAGGGTTGGTAAGGCGTGTacc                                          >  1:230605/1‑147 (MQ=255)
                                                                                                      aTAGCTGAGCCAGTCGTGGGTGATACTTTGCGGCAGGTTACCCAAGGCGAAGACGTTGACCACATTGGCGCCGACACCCGCCACCACGGCCACTGCACCCAGCGGGATCTCGTAGAt                                                                       >  2:164430/1‑117 (MQ=255)
                                                                                                      aTAGCTGAGCCAGTCGTGGGTGATACTTTGCGGCAGGTTACCCAAGGCGAAGACGTTGACCACATTGGCGCCGACACCCGCCACCACGGCCACTGCACCCAGCGGGATCTCGTAGAt                                                                       <  1:164430/117‑1 (MQ=255)
                                                                                                      aTAGCTGAGCCAGTCGTGGGTGATACTTTGCGGCAGGTTACCCAAGGCGAAGACGTTGACCACATTGGCGCCGACACCCGCCACCACGGCCACTGCACCCAGCGGGATCTCGTAGATCTCCCGCCAGGGTTGGTAAGGCGTGTAGCGGt                                       <  4:194947/149‑1 (MQ=255)
                                                                                                             aGCCAGTCGTGGGTGATACTTTGCGGCAGGTTACCCAAGGCGAAGACGTTGACCACATTGGCGCCGACACCCGCCACCACGGCCACTGCACCCAGCGGGATCTCGTAGATCTCCCGCCAGGGTTGGTAAGGCGTGTAGCGGTCATaa                                  <  3:67816/147‑1 (MQ=255)
                                                                                                               ccAGTCGTGGGTGATACTTTGCGGCAGGTTACCCAAGGCGAAGACGTTGACCACATTGGCGCCGACACCCGCCACCACGGCCACTGCACCCAGCGGGATCTCGTAGATCTCCCGCCAGGGTTGGTAAGGCGTGTAGCGGTCATAacgac                              >  4:782/1‑149 (MQ=255)
                                                                                                               ccAGTCGTGGGTGATACTTTGCGGCAGGTTACCCAAGGCGAAGACGTTGACCACATTGGCGCCGACACCCGCCACCACGGCCACTGCACCCAGCGGGATCTCGTAGATCTCCCGCCAGGGTTGGTAAGGCGTGTAGCGGTCATAacgac                              >  4:4713/1‑149 (MQ=255)
                                                                                                                cAGTCGTGGGTGATACTTTGCGGCAGGTTACCCAAGGCGAAGACGTTGACCACATTGGCGCCGACACCCGCCACCACGGCCACTGCACCCAGCGGGATCTCGTAGATCTCCCGCCAGGGTTGGTAAGGCGTGTAGCGGTCATAacgacg                             <  2:230605/149‑1 (MQ=255)
                                                                                                                        ggTGATACTTTGCGGCAGGTTACCCAAGGCGAAGACGTTGACCACATTGGCGCCGACACCCGCCACCACGGCCACTGCACCCAGCGGGATCTCGTAGATCTCCCGCCAGGGTTGGTAAGGCGTGTAGCGGTCATAACGACGGGTCACtt                     >  1:201814/1‑149 (MQ=255)
                                                                                                                                   gCGGCAGGTTACCCAAGGCGAAGACGTTGACCACATTGGCGCCGACACCCGCCACCACGGCCACTGCACCCAGCGGGATCTCGTAGATCTCCCGCCAGGGTTGGTAAGGCGTGTAGCGGTCATAACGACGGGTCACTTCGAATTCGGt           <  8:61371/148‑1 (MQ=255)
                                                                                                                                          gTTACCCAAGGCGAAGACGTTGACCACATTGGCGCCGACACCCGCCACCACGGCCACTGCACCCAGCGGGATCTCGTAGATCTCCCGCCAGGGTTGGTAAGGCGTGTAGCGGTCATAacgac                              <  6:4147/122‑1 (MQ=255)
                                                                                                                                          gTTACCCAAGGCGAAGACGTTGACCACATTGGCGCCGACACCCGCCACCACGGCCACTGCACCCAGCGGGATCTCGTAGATCTCCCGCCAGGGTTGGTAAGGCGTGTAGCGGTCATAacgac                              >  5:4147/1‑122 (MQ=255)
                                                                                                                                           ttACCCAAGGCGAAGACGTTGACCACATTGGCGCCGACACCCGCCACCACGGCCACTGCACCCAGCGGGATCTCGTAGATCTCCCGCCAGGGTTGGTAAGGCGTGTAGCGGTCATAACGACGGGTCACTTCGAATTCGGTGACTTCGaa  <  1:162474/149‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                                 
TTCCTCGCGCTTGCCTTTCTGCACTTCGCTGATCCCGGCCAGGTTCTGCTGGGCGCGGCCGTTGGATGGCACGTTCATGAACGGGTTGAGCCCATCCACGCCATAGCTGAGCCAGTCGTGGGTGATACTTTGCGGCAGGTTACCCAAGGCGAAGACGTTGACCACATTGGCGCCGACACCCGCCACCACGGCCACTGCACCCAGCGGGATCTCGTAGATCTCCCGCCAGGGTTGGTAAGGCGTGTAGCGGTCATAACGACGGGTCACTTCGAATTCGGTGACTTCGAA  >  NC_002947/1382797‑1383084

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑