| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,394,408 | C→G | 10.3% | R49G (CGC→GGC) | ruvB → | Holliday junction ATP‑dependent DNA helicase RuvB |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,394,408 | 0 | C | G | 10.3% | 109.0 / 5.1 | 39 | R49G (CGC→GGC) | ruvB | Holliday junction ATP‑dependent DNA helicase RuvB |
| Reads supporting (aligned to +/- strand): ref base C (17/18); new base G (2/2); total (19/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.67e-01 | |||||||||||
CCAAGTGATCGAAGCCGACCGCCTGATCGCCGCCAGTGGCCGCGACCGCGAAGAAGTCCAGGACCGTGCGATTCGCCCGCTGAGCCTGGACGAGTACATCGGCCAGCCGGTGGTGCGCGAGCAGATGGCGCTGTTCATCCAGGCCGCCCGCGGCCGCGGCGAGTCGCTTGACCACACGCTGATCTTCGGC‑CCGCCCGGCCTGGGCAAGACCACCCTGGCCAACATCATCGCCCATGAAATGGGCGTGTCGGTGAAGAGCACTTCGGGGCCGATTCTCGAGCGCCCCGGCGACCTGG > NC_002947/1394260‑1394555 | ccAAGTGATCGAAGCCGACCGCCTGATCGCCGCCAGTGGCCGCGACCGCGAAGAAGTCCAGGACCGTGCGATTCGCCCGCTGAGCCTGGACGAGTACATCGGCCAGCCGGTGGTGCGCGAGCAGATGGCGCTGTTCATCCAGGccgccc > 5:210118/1‑149 (MQ=255) cGAAGCCGACCGCCTGATCGCCGCCAGTGGCCGCGACCGCGAAGAAGTCCAGGACCGTGCGATTCGCCCGCTGAGCCTGGACGAGTACATCGGCCAGCCGGTGGTGCGCGAGCAGATGGCGCTGTTCATCCAGGCCGCccgcggccgcg > 1:122809/1‑149 (MQ=255) gAAGCCGACCGCCTGATCGCCGCCAGTGGCCGCGACCGCGAAGAAGTCCAGGACCGTGCGATTCGCCCGCTGAGCCTGGACGAGTACATCGGCCAGCCGGTGGTGCGCGAGCAGATGGCGCTGTTCATCCAGGCCGCgggcggcctgga < 2:164780/149‑5 (MQ=255) caGCCGCCCGCGTGATCGCCGCCCGGGGCCGCGACCGCGAAGATGTCCAGGACCGTGCGATTCGCCCGCTGAGCCTGGACGAGTACATCGGCCAGCCGGTGGTGGGCGAGCTGATGGCGCTGTTCATCCAGGCCGCCcgcggccgcggc < 8:215525/148‑1 (MQ=255) aaGCCGACCGCCTGATCGCCGCCAGTGGCCGCGACCGCGAAGAAGTCCAGGACCGTGCGATTCGCCCGCTGAGCCTGGACGAGTACATCGGCCAGCCGGTGGTGCGCGAGCAGATGGCGCTGTTCATCCAGGCCGCCcgcggccgcggc > 2:215545/1‑149 (MQ=255) gCCGACCGCCTGATCGCCGCCAGTGGCCGCGACCGCGAAGAAGTCCAGGACCGTGCGATTCGCCCGCTGAGCCTGGACGAGTACATCGGCCAGCCGGTGGTGCGCGAGCAGATGGCGCTGTTCATCCAGGCCGCCCGCGGCCGCGGCGa < 5:133601/149‑1 (MQ=255) cGACCGCCTGATCGCCGCCAGTGGCCGCGACCGCGAAGAAGTCCAGGACCGTGCGATTCGCCCGCTGAGCCTGGACGAGTACATCGGCCAGCCGGTGGTGCGCGAGCAGATGGCGCTGTTCATCCAGGCCGCCCGCGGCCGCGGCGAGt < 3:51576/149‑1 (MQ=255) aCCGCCTGATCGCCGCCAGTGGCCGCGACCGCGAAGAAGTCCAGGACCGTGCGATTCGCCCGCTGAGCCTGGACGAGTACATCGGCCAGCCGGTGGTGCGCGAGCAGATGGCGCTGTTCATCCAGGCCGCCCGCGGCCGCGGCGa > 4:199078/1‑145 (MQ=255) aCCGCCTGATCGCCGCCAGTGGCCGCGACCGCGAAGAAGTCCAGGACCGTGCGATTCGCCCGCTGAGCCTGGACGAGTACATCGGCCAGCCGGTGGTGCGCGAGCAGATGGCGCTGTTCATCCAGGCCGCCCGCGGCCGCGGCGa < 3:199078/145‑1 (MQ=255) ccGCCTGATCGCCGCCAGTGGCCGCGACCGCGAAGAAGTCCAGGACCGTGCGATTCGCCCGCTGAGCCTGGACGAGTACATCGGCCAGCCGGTGGTGCGCGAGCAGATGGCGCTGTTCATCCAGGCCGCCCGCGGCCGCGGCGAGTCGc < 2:41784/149‑1 (MQ=255) ccTGATCGCCGCCAGTGGCCGCGACCGCGAAGAAGTCCAGGACCGTGCGATTCGCCCGCTGAGCCTGGACGAGTACATCGGCCAGCCGGTGGTGCGCGAGCAGATGGCGCTGTTCATCCAGGCCGCCCGCGGCCGCGGCGAGTCGCtt > 5:2640/1‑148 (MQ=255) ccTGATCGCCGCCAGTGGCCGCGACCGCGAAGAAGTCCAGGACCGTGCGATTCGCCCGCTGAGCCTGGACGAGTACATCGGCCAGCCGGTGGTGCGCGAGCAGATGGCGCTGTTCATCCAGGCCGCCCGCGGCCGCGGCGAGTCGCTTg > 7:4071/1‑149 (MQ=255) cTGATCGCCGCCAGTGGCCGCGACCGCGAAGAAGTCCAGGACCGTGCGATTCGCCCGCTGAGCCTGGACGAGTACATCGGCCAGCCGGTGGTGCGCGAGCAGATGGCGCTGTTCATCCAGGCCGCCCGCGGCCGCGGCGAGTCGCTTGa < 6:210118/149‑1 (MQ=255) gccgccAGTGGCCGCGACCGCGAAGAAGTCCAGGACCGTGCGATTCGCCCGCTGAGCCTGGACGAGTACATCGGCCAGCCGGTGGTGCGCGAGCAGATGGCGCTGTTCATCCAGGCCGCCCGCGGCCGCGGCGAGTCGCTTGACCacac > 7:174553/1‑149 (MQ=255) gCCGCGACCGCGAAGAAGTCCAGGACCGTGCGATTCGCCCGCTGAGCCTGGACGAGTACATCGGCCAGCCGGTGGTGCGCGAGCAGATGGCGCTGTTCATCCAGGCCGCgggcggcctggat < 3:109528/122‑6 (MQ=255) gCCGCGACCGCGAAGAAGTCCAGGACCGTGCGATTCGCCCGCTGAGCCTGGACGAGTACATCGGCCAGCCGGTGGTGCGCGAGCAGATGGCGCTGTTCATCCAGGCCGCgggcggcctggat > 4:109528/1‑117 (MQ=255) gCCGCGACCGCGAAGAAGTCCAGGACCGTGCGATTCGCCCGCTGAGCCTGGACGAGTACATCGGCCAGCCGGTGGTGCGCGAGCAGATGGCGCTGTTCATCCAGGCCGCCCGCGGCCGCGGCGAGTCGCTTGACCACACGCTGATCTTc < 3:19324/149‑1 (MQ=255) ccgcgaccgcgaAGAAGTCCAGGACCGTGCGATTCGCCCGCTGAGCCTGGACGAGTACATCGGCCAGCCGGTGGTGCGCGAGCAGATGGCGCTGTTCATCCAGGCCGCCCGCGGCCGCGGCGAGTCGCTTGACCACACGCTGATCTTCg < 8:174553/149‑1 (MQ=255) gcgaAGAAGTCCAGGACCGTGCGATTCGCCCGCTGAGCCTGGACGAGTACATCGGCCAGCCGGTGGTGCGCGAGCAGATGGCGCTGTTCATCCAGGCCGCCCGCGGCCGCGGCGAGTCGc > 4:195848/1‑120 (MQ=255) agTCCAGGACCGTGCGATTCGCCCGCTGAGCCTGGACGAGTACATCGGCCAGCCGGTGGTGCGCGAGCAGATGGCGCTGTTCATCCAGGCCGCCCGCGGCCGCGGCGAGTCGCTTGACCACACGCTGATCTTCGGC‑CCGCCCGGCCTg < 6:2640/148‑1 (MQ=255) tCCAGGACCGTGCGATTCGCCCGCTGAGCCTGGACGAGTACATCGGCCAGCCGGTGGTGCGCGAGCAGATGGCGCTGTTCATCCAGGCCGCCCGCGGCCGCGGCGAGTCGCTTGACCACACGCTGATCTTCGGC‑CCGCCCGGCCTggg > 3:112893/1‑148 (MQ=255) aGGACCGTGCGATTCGCCCGCTGAGCCTGGACGAGTACATCGGCCAGCCGGTGGTGCGCGAGCAGATGGCGCTGTTCATCCAGGCCGCCCGCGGCCGCGGCGAGTCGCTTGACCACACGCTGATCTTCGGC‑CCGCCCGGCCTGGGc > 1:223404/1‑146 (MQ=255) tGCGATTCGCCCGCTGAGCCTGGACGAGTACATCGGCCAGCCGGTGGTGCGCGAGCAGATGGCGCTGTTCATCCAGGCCGCCGGCGGCCGCGGCGAGTCGCTTGACCACACGCTg < 7:46796/115‑1 (MQ=255) tGCGATTCGCCCGCTGAGCCTGGACGAGTACATCGGCCAGCCGGTGGTGCGCGAGCAGATGGCGCTGTTCATCCAGGCCGCCGGCGGCCGCGGCGAGTCGCTTGACCACACGCTg > 8:46796/1‑115 (MQ=255) ttCGCCCGCTGAGCCTGGACGAGTACATCGGCCAGCCGGTGGTGCGCGAGCAGATGGCGCTGTTCATCCAGGCCGCCCGCGGCCGCGGCGAGTCGCTTGACCACACGCTGATCTTCGGC‑CCGCCCGGCCTGGGCAAGaccac < 8:222997/142‑1 (MQ=255) ttCGCCCGCTGAGCCTGGACGAGTACATCGGCCAGCCGGTGGTGCGCGAGCAGATGGCGCTGTTCATCCAGGCCGCCCGCGGCCGCGGCGAGTCGCTTGACCACACGCTGATCTTCGGC‑CCGCCCGGCCTGGGCAAGaccac > 7:222997/1‑142 (MQ=255) gCTGAGCCTGGACGAGTACATCGGCCAGCCGGTGGTGCGCGAGCAGATGGCGCTGTTCATCCAGGCCGCCCGCGGCCGCGGCGAGTCGCTTGACCACACGCTGATCTTCGGC‑CCGCCCGGCCTGGGCAAGACCACCCTGGCCAAcatca > 5:118017/1‑149 (MQ=255) ccTGGACGAGTACATCGGCCAGCCGGTGGTGCGCGAGCAGATGGCGCTGTTCATCCAGGCCGCCCGCGGCCGCGGCGAGTCGCTTGACCACACGCTGATCTTCGGC‑CCGCCCGGCCTgg > 3:148224/1‑119 (MQ=255) ccTGGACGAGTACATCGGCCAGCCGGTGGTGCGCGAGCAGATGGCGCTGTTCATCCAGGCCGCCCGCGGCCGCGGCGAGTCGCTTGACCACACGCTGATCTTCGGC‑CCGCCCGGCCTgg < 4:148224/119‑1 (MQ=255) ccTGGACGAGTACATCGGCCAGCCGGTGGTGCGCGAGCAGATGGCGCTGTTCATCCAGGCCGCCCGCGGCCGCGGCGAGTCGCTTGACCACACGCTGATCTTCGGC‑CCGCCCGGCCTGGGCAAGACCACCCTGGCCAACATCATCGcc > 5:137169/1‑148 (MQ=255) aTCGGCCAGCCGGTGGTGCGCGAGCAGATGGCGCTGTTCATCCAGGCCGCCCGCGGCCGCGGCGAGTCGCTTGACCACACGCTGATCTTCGGC‑CCGCCCGGCCTGGGCAAGACCACCCTGGCCAACATCATCGCCCATTTCATGGGCga < 7:98032/149‑2 (MQ=255) aTCGGCCAGCCGGTGGTGCGCGAGCAGATGGAGCTGTTCATCCAGGCCGCCCGCGGCCGCGGCGAGTCGCTTGACCACACGCTGATCTTCGGC‑CCGCCCCGCCTGGGCAAGACCACCCTGGCCAACATCATCGCCCATGAAATGGGCgt > 4:97658/1‑149 (MQ=255) ggCCAGCCGGTGGTGCGCGAGCAGATGGCGCTGTTCATCCAGGCCGCCCGCGGCCGCGGCGAGTCGCTTGACCACACGCTGATCTTCGGC‑CCGCCCGGCCTGGGCAAGACCACCCTGGCCAACATCATCGCCCATTTCATGGGCgatg < 6:226822/148‑4 (MQ=255) aGCCGGTGGTGCGCGAGCAGATGGCGCTGTTCATCCAGGCCGCCCGCGGCCGCGGCGAGTCGCTTGACCACACGCTGATCTTCGGC‑CCGCCCGGCCTGGGCAAGACCACCCTGGCCAACATCATCGCCCATGAAATGGGCGTGTCGGTg < 2:237763/149‑1 (MQ=255) cccGGTGGTGCGCGAGCAGATCGCGCTGATCATCCACGCCGCCCGCCGCAGCGGCTAGACGCTTGACCACACGCTGATCTTCGGC‑AAGCCCGGCCTGGGCAAGACCACCCTGGCCAACATCAACTCCCAAGAAAACGGCGAGTCGGTg > 5:697/2‑148 (MQ=255) ccGGTGGTGCGCGAGCAGATGGCGCTGTTCATCCAGGCCGCCCGCGGCCGCGGCGAGTCGCTTGACCACACGCTGATCTTCGGC‑CCGCCCGGCCTGGGCAAGACCACCCTGGCCAACATCATCGCCCATGAAATGGGCGTGTCGGTGaa < 6:137169/149‑1 (MQ=255) ggtggtGCGCGAGCAGATGGCGCTGTTCATCCAGGCCGCCCGCGGCCGCGGCGAGTCGCTTGACCACACGCTGATCTTCGGCTACGCCCGGCCTGGGCAAGACCACCCTGGCCAACATCATCGCCCATGAAATGGGCGTGTCGGTGAag < 1:215545/149‑1 (MQ=255) ggtggtGCGCGAGCAGATGGCGCTGTTCATCCAGGCCGCCCGCGGCCGCGGCGAGTCGCTTGACCACACGCTGATCTTCGGC‑CCGCCCGGCCTGGGCAAGACCACCCTGGCCAACATCATCGCCCATGAAATGGGCGTGTCGGTGAaga < 8:4071/149‑1 (MQ=255) ggtgCGCGAGCAGATGGCGCTGTTCATCCAGGCCGCCCGCGGCCGCGGCGAGTCGCTTGACCACACGCTGATCTTCGGC‑CCGCCCGGCCTGGGCAAGACCACCCTGGCCAACATCATCGCCCATGAAATGGGCGTGTCGGTGAAGAGCa < 4:31138/149‑1 (MQ=255) aGCAGATGGCGCTGTTCATCCAGGCCGCCCGCGGCCGCGGCGAGTCGCTTGACCACACGCTGATCTTCGGC‑CCGCCCGGCCTGGGCAAGACCACCCTGGCCAACATCATCGCCCATGAAATGGGCGTGTCGGTGAAGAGCACTTCgggg > 1:213270/1‑149 (MQ=255) aGCAGATGGCGCTGTTCATCCAGGCCGCCCGCGGCCGCGGCGAGTCGCTTGACCACACGCTGATCTTCGGC‑CCGCCCGGCCTGGGCAAGACCACCCTGGCCAACATCATCGCCCATGAAATGGGCGTGTCGGTGAAGAGCACTTCgggg < 2:122809/149‑1 (MQ=255) aTGGCGCTGTTCATCCAGGCCGCCCGCGGCCGCGGCGAGTAGGTTGACCACACGCTGATCTTCGGC‑CCGCCCGGCCTGGGCAAGACCACCCTGGCCAACATCATCGCCCATGAAATGGGCGTGTCGGTGAAGAGCACTTCGGGGCCGAt > 1:52637/1‑149 (MQ=255) gcTGTTCATCCAGGCCGCCCGCGGCCGCGGCGAGTCGCTTGACCACACGCTGATCTTCGGC‑CCGCCCGGCCTGGGCAAGACCACCCTGGCCAACATCATCGCCCATGAAATGGGCGTGTCGGTGAAGAGCACTTCGGGGCCGATtctc > 1:249544/1‑148 (MQ=255) ccgcggCCGCGGGCGGCCGCGGCGAGTCGCTTGACCACACGCTGATCTTCGGC‑CCGCCCGGCCTGGGCAAGACCACCCTGGCCAACATCATCGCCCATGAAATGGGCGTGTCgg < 4:259236/110‑1 (MQ=255) ccgcggCCGCGGGCGGCCGCGGCGAGTCGCTTGACCACACGCTGATCTTCGGC‑CCGCCCGGCCTGGGCAAGACCACCCTGGCCAACATCATCGCCCATGAAATGGGCGTGTCgg > 3:259236/5‑114 (MQ=255) ccAGGCCGCCCGCGGCCGCGGCGAGTCGCTTGACCACACGCTGATCTTCGGC‑CCGCCCGGCCTGGGCAAGACCACCCTGGCCAACATCATCGCCCATGAAATGGGCGTGTCGGTGAAGAGCACTTCGGGGCCGATTCTCGAGCGCCCCg > 1:83182/1‑149 (MQ=255) cgcccgcGGCCGCGGCGAGTCGCTTGACCACACGCTGATCTTCGGC‑CCGCCCGGCCTGGGCAAGACCACCCTGGCCAACATCATCGCCCATGAAATGGGCGTGTCGGTGAAGAGCACTTCGGGGCCGATTCTCGAGCGCCCCGGCGAcc > 3:80229/1‑149 (MQ=255) cgcccgcGGCCGCGGCGAGTCGCTTGACCACACGCTGATCTTCGGC‑CCGCCCGGCCTGGGCAAGACCACCCTGGCCAACATCATCGCCCATGAAATGGGCGTGTCGGTGAAGAGCACTTCGGGGCCGATTCTCGAGCGCCCCGGCGAcc < 2:223404/149‑1 (MQ=255) cgcccgcGGCCGCGGCGAGTCGCTTGACCACACGCTGATCTTCGGC‑CCGCCCGGCCTGGGCAAGACCACCCTGGCCAACATCATCGCCCATGAAATGGGCGTGTCGGTGAAGAGCACTTCGGGGCCGATTCTCGAGCGCCCCGGCGAcc > 2:132015/1‑149 (MQ=255) ccgcggccgcggCGAGTCGCTTGACCACACGCTGATCTTCGGC‑CCGCCCGGCCTGGGCAAGCCCACCCTGGCCAACATCATCGCCCATGaa > 5:138792/1‑91 (MQ=255) ccgcggccgcggCGAGTCGCTTGACCACACGCTGATCTTCGGC‑CCGCCCGGCCTGGGCAAGACCACCCTGGCCAACATCATCGCCCATGaa < 6:138792/91‑1 (MQ=255) ccgcggccgcggCGAGTCGCTTGACCACACGCTGATCTTCGGC‑CCGCCCGGCCTGGGCAAGACCACCCTGGCCAACATCATCGCCCATGAAATGGGCGTGTCGGTGAAGAGCACTTCGGGGCCGATtc > 5:269886/1‑128 (MQ=255) ccgcggccgcggCGAGTCGCTTGACCACACGCTGATCTTCGGC‑CCGCCCGGCCTGGGCAAGACCACCCTGGCCAACATCATCGCCCATGAAATGGGCGTGTCGGTGAAGAGCACTTCGGGGCCGATtc < 6:269886/128‑1 (MQ=255) ccgcggccgcggCGAGTCGCTTGACCACACGCCGATCTTCGGC‑CCGCCCGGCCTGGGCAAGACCACCCTGGCCAACATCATCGCCCATGAAATGGGCGTGTCGGTGAAGAGCACTTCGGGGCCGATTCTCGAGCGCCCCGGCGACCTgg > 2:206661/1‑149 (MQ=255) | CCAAGTGATCGAAGCCGACCGCCTGATCGCCGCCAGTGGCCGCGACCGCGAAGAAGTCCAGGACCGTGCGATTCGCCCGCTGAGCCTGGACGAGTACATCGGCCAGCCGGTGGTGCGCGAGCAGATGGCGCTGTTCATCCAGGCCGCCCGCGGCCGCGGCGAGTCGCTTGACCACACGCTGATCTTCGGC‑CCGCCCGGCCTGGGCAAGACCACCCTGGCCAACATCATCGCCCATGAAATGGGCGTGTCGGTGAAGAGCACTTCGGGGCCGATTCTCGAGCGCCCCGGCGACCTGG > NC_002947/1394260‑1394555 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |