Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 182,035 A→T 10.2% intergenic (+24/+41) PP_5SB → / ← PP_0160 5S ribosomal RNA/ferrioxamine receptor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947182,0350AT10.2% 135.9 / 5.7 49intergenic (+24/+41)PP_5SB/PP_01605S ribosomal RNA/ferrioxamine receptor
Reads supporting (aligned to +/- strand):  ref base A (23/21);  new base T (2/3);  total (25/24)
Fisher's exact test for biased strand distribution p-value = 6.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.71e-01

TTTCTTGACGACCATAGAGCATTGGAACCACCTGATCCCATCCCGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCTGACCGTGGCGCTCACATTGCGCTCTTCACCCATGTAGCAGTAGTTCAGGCTGGCACATGAGGTGATGTAGCTTTCATTGGTAAGGTTGTTGG  >  NC_002947/181891‑182182
                                                                                                                                                |                                                                                                                                                   
tttCTTGACGACCATAGAGCATTGGAACCACCTGATCCCATCCCGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGATg                                                                                                                                                   >  8:147491/1‑147 (MQ=255)
   cTTGACGACCATAGAGCATTGGAACCACCTGATCCCATCCCGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGATGCAGa                                                                                                                                               <  7:147491/148‑1 (MQ=255)
      gacgacCATAGAGCATTGGAACCACCTGATCCCATCCCGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGATGCAGACa                                                                                                                                             <  2:47047/147‑1 (MQ=255)
      gacgacCATAGAGCATTGGAACCACCTGATCCCATCCCGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGATGCAGACAg                                                                                                                                            <  6:132581/148‑1 (MQ=255)
      gacgacCATAGAGCATTGGAACCACCTGATCCCATCCCGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCATCGCAGACAg                                                                                                                                            <  2:57328/148‑1 (MQ=255)
            cATAGAGCATTGGAACCACCTGATCCCATCCCGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGATGCAGACAGGGGCttt                                                                                                                                     <  3:172166/149‑1 (MQ=255)
              tAGAGCATTGGAACCACCTGATCCCATCCCGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGATGCAGACAgg                                                                                                                                           >  1:173368/1‑141 (MQ=255)
              tAGAGCATTGGAACCACCTGATCCCATCCCGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGATGCAGACAgg                                                                                                                                           <  2:173368/141‑1 (MQ=255)
              tAGAGCATTGGAACCACCTGATCCCATCCCGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGATGCAGACAGGGGCTTTg                                                                                                                                    >  4:131482/1‑148 (MQ=255)
                        gAACCACCTGATCCCATCCCGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGATGCAGACAGGGGCTTTGTCTTTTCCggg                                                                                                                         >  1:168140/1‑149 (MQ=255)
                             accTGATCCCATCCCGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGATGCAGACAGGGGCTTTGTCTTTTCCgg                                                                                                                          >  3:152519/1‑143 (MQ=255)
                             accTGATCCCATCCCGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGATGCAGACAGGGGCTTTGTCTTTTCCgg                                                                                                                          <  4:152519/143‑1 (MQ=255)
                                   tcccatcccGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCg                                                                                                              <  3:131482/149‑1 (MQ=255)
                                    cccatcccGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCgg                                                                                                             >  7:201511/1‑149 (MQ=255)
                                      catcccGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGtt                                                                                                           <  2:168140/149‑1 (MQ=255)
                                       atcccGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTa                                                                                                          >  6:209847/1‑149 (MQ=255)
                                                         aaaCGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCTGACCg                                                                                         <  8:201511/148‑1 (MQ=255)
                                                              aTGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCTGACCGTGgcgc                                                                                   >  7:109838/1‑149 (MQ=255)
                                                                   tCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCTGACCGTGGCGCTcaca                                                                              >  3:247251/1‑149 (MQ=255)
                                                                       cGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCTGACCGTGGCGCTCACATTgc                                                                          >  2:226410/1‑149 (MQ=255)
                                                                        gATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCTGACCGTGGCGCTCACATTgcg                                                                         >  7:197701/1‑149 (MQ=255)
                                                                                  tgGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCTGACCGTGGCGCTca                                                                                <  5:226452/132‑1 (MQ=255)
                                                                                  tgGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCTGACCGTGGCGCTca                                                                                >  6:226452/1‑132 (MQ=255)
                                                                                    gggTTTCGCCATGGGAGAGTATGTCATCGACAAGATTCAAACCCAAAGCCCCTGTCTGCGATGCAGACAGGGGCTTTGTCTTTTACGGGTTTCAATATCGGTTAGAACTGATAGCTGACCGTGTCGCTCACATTGCGCTCTTCACCCAt                                                             <  7:226448/149‑1 (MQ=255)
                                                                                           cccATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCTGACCGTGGCGCTCACATTGCGCTCTTCACCCATGTAGCag                                                      <  8:197701/149‑1 (MQ=255)
                                                                                             cATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCTGACCGTGGCGCTCACATTGCGCTCTTCACCCATGTAGCagta                                                    >  7:28218/1‑149 (MQ=255)
                                                                                             cATGGGAGACTAGGTCCTCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCTGACCGTGGCGCTCACATTGCGCTCTTCACCCATGTAGCagta                                                    <  4:173867/149‑1 (MQ=255)
                                                                                                 tgAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCTGACCGTGGCGCTCACATTGCGCTCTTCACCCATGTAGCAGTAGtt                                                 <  8:28218/148‑1 (MQ=255)
                                                                                                           tCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCTGACCGTGGCGCTCACATTGCGCTCTTCACCCATGTa                                                          <  5:242513/129‑1 (MQ=255)
                                                                                                           tCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCTGACCGTGGCGCTCACATTGCGCTCTTCACCCATGTa                                                          >  6:242513/1‑129 (MQ=255)
                                                                                                                         cAAACCCAAAGCCCCTGTCTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCTGACCGTGGCGCTCACATTGCGCTCTTCACCCATGTAGCAGTAGTTCAGGCTGGCACATGAGGTGATGTa                         <  5:209847/148‑1 (MQ=255)
                                                                                                                          aaaCCCAAAGCCCCTGTCTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCTGACCGTGGCGCTCACATTGCGCTCTTCACCCATGTAGCAGTAGTTCAGGCTGGCACATGAGGTGATGTAGc                       >  6:211917/1‑149 (MQ=255)
                                                                                                                              ccAAAGCCCCTGTCTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCTGACCGTGGCGCTCACATTGCGCTCTTCACCCATGTAGCAGTAGTTCAGGCTGGCACATGAGGTGATGTAGCttt                    >  4:171078/1‑148 (MQ=255)
                                                                                                                               ctaaGCCCCTGTCTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCTGACCGTGGCGCTCACATTGCGCTCTTCACCCATGTAGCAGTAGTTCAGGCTGGCACATGAGGTGAt                            <  1:106261/137‑1 (MQ=255)
                                                                                                                                   gCCCCTGTCTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCTGACCGTGGCGCTCACATTGCGCTCTTCACCCATGTAGCAGTAGTTCAGGCTGGCACATGAGGTGATGTAGCTTTCATTgg              >  5:225876/1‑149 (MQ=255)
                                                                                                                                    ccccTGTCTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCTGACCGTGGCGCTCACATTGCGCTCTTCACCCATGTAGCAGTAGTTCAGGCTGGCACATGAGGTGATGTAGCTTTCATTgg              >  1:236665/1‑148 (MQ=255)
                                                                                                                                    ccccTGTCTGCATCGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCTGACCGTGGCGCTCACATTGCGCTCTTCACCCATGTAGCAGTAGTTCAGGCTGGCACATGa                                  <  2:5528/128‑1 (MQ=255)
                                                                                                                                    ccccTGTCTGCATCGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCTGACCGTGGCGCTCACATTGCGCTCTTCACCCATGTAGCAGTAGTTCAGGCTGGCACATGa                                  >  1:5528/1‑128 (MQ=255)
                                                                                                                                     cccTGTCTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCTGACCGTGGCGCTCACATTGCGCTCTTCACCCATGTAGCAGTAGTTCAGGCTgg                                         <  3:209792/120‑1 (MQ=255)
                                                                                                                                     cccTGTCTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCTGACCGTGGCGCTCACATTGCGCTCTTCACCCATGTAGCAGTAGTTCAGGCTgg                                         >  4:209792/1‑120 (MQ=255)
                                                                                                                                     cccTGTCTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCTGACCGTGGCGCTCACATTGCGCTCTTCACCCATGTAGCAGTAGTTCAGGCTGGCACATGAGGTGATGTAGCTTTCATTGGt             >  2:175387/1‑148 (MQ=255)
                                                                                                                                     cccTGTCTGCATCGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCTGACCGTGGCGCTCACATTGCGCTCTTCACCCATGTAGCAGTAGTTCAGGCTg                                          <  2:188803/119‑1 (MQ=255)
                                                                                                                                     cccTGTCTGCATCGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCTGACCGTGGCGCTCACATTGCGCTCTTCACCCATGTAGCAGTAGTTCAGGCTg                                          >  1:188803/1‑119 (MQ=255)
                                                                                                                                      ccTGTCTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCTGACCGTGGCGCTCACATTGCGCTCTTCACCCATGTAGCAGTAGTTCAGGCTGGCACATGAGGTGATGTAGCTTTCATTGGt             >  2:177669/1‑147 (MQ=255)
                                                                                                                                      ccTGTCTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCTGACCGTGGCGCTCACATTGCGCTCTTCACCCATGTAGCAGTAGTTCAGGCTGGCACATGAGGTGATGTAGCTTTCATTGGTaa           <  1:175387/149‑1 (MQ=255)
                                                                                                                                       cTGTCTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCTGACCGTGGCGCTCACATTGCGCTCTTCACCCATGTAGCAGTAGTTCAGGCTGGCACATGAGGTGATGTAGCTTTCATTGGTAAg          <  6:225876/149‑1 (MQ=255)
                                                                                                                                       cTGTCTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCTGACCGTGGCGCTCACATTGCGCTCTTCACCCATGTAGCAGTAGTTCAGGCTGGCACATGAGGTGATGTAGCTTTCATTGGTAAg          >  7:122087/1‑149 (MQ=255)
                                                                                                                                       cTGTCTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGGTTCAATATCGGTTAGAACTGATAGCTGACCGTGGCGCTCACATTGCGCTCTTCACCCATGTAGCAGTAGTTCAGGCTGGCACATGAGGTGATGTAGCTTTCATTGGTAAg          <  8:122087/149‑1 (MQ=255)
                                                                                                                                               gATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCTGACCGTGGCGCTCACATTGCGCTCTTCACCCATGTAGCAGTAGTTCAGGCTGGCACATGAGGTGATGTAGCTTTCATTGGTAAGGTTGTTgg  >  2:210607/1‑149 (MQ=255)
                                                                                                                                                |                                                                                                                                                   
TTTCTTGACGACCATAGAGCATTGGAACCACCTGATCCCATCCCGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCTGACCGTGGCGCTCACATTGCGCTCTTCACCCATGTAGCAGTAGTTCAGGCTGGCACATGAGGTGATGTAGCTTTCATTGGTAAGGTTGTTGG  >  NC_002947/181891‑182182

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑