Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,492,266 T→C 7.5% intergenic (+27/+68) cysNC → / ← imm sulfate adenylyltransferase subunit 1/adenylyl‑sulfate kinase/colicin‑E7 immunity protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,492,2660TC 7.5% 149.8 / 2.7 54intergenic (+27/+68)cysNC/immsulfate adenylyltransferase subunit 1/adenylyl‑sulfate kinase/colicin‑E7 immunity protein
Reads supporting (aligned to +/- strand):  ref base T (24/25);  new base C (2/2);  total (27/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.35e-01

TTCCCGTTCGATGTGCCGCTGGATGCTGATCTTGTGATCGACACCCAGGCCACCAGCGTGGACGAAGGCGTGAAACAGGTGCTGGACGTGCTGCGTCAGCGTGGCGCGATCTAAGCGCTAGCCTGCAAGGAAAACCCCGCTTCGGCGGGGTTTTTTCTATTGCTCAGTTCGGGTGAAAAGGCCCTCAGAGTTCAGGGCCTTGCTCGTGTTAGCTGGGCTTGAAACCGTCTTTACCGTTGGCTAGCCGCCATTGCTTGACTATTTCTAGTACTTTTTCAGGTTTACC  >  NC_002947/1492126‑1492411
                                                                                                                                            |                                                                                                                                                 
ttCCCGTTCGATGTGCCGCTGGATGCTGATCTTGAGATCGACACCCACGCCACCAGAGTGGAAGAAGGAGTGAAACACGTGCTGGAAGTGCTGCGTCACCGTGGCGCGATCAAAGACCATGCCTACAAGCAAAACCCCGCTAAGGCggg                                                                                                                                           >  8:122044/1‑149 (MQ=255)
    cGTTCGATGTGCCGCTGGATGCTGATCTTGTGATCGACACCCAGGCCACCAGCGTGGACGAAGGCGTGAAACAGGTGCTGGACGTGCTGCGTCAGCGTGGCGCGATCTAAGCGCTAGCCTGCAAGGAAAACCCCGCTTCGGCGGGGttt                                                                                                                                       >  4:247895/1‑149 (MQ=255)
    cGTTCGATGTGCCGCTGGATGCTGATCTTGTGATCGACACCCAGGCCACCAGCGTGGACGAAGGCGTGAAACAGGTGCTGGACGTGCTGCGTCAGCGTGGCGCGATCTAAGCGCTAGCCTGCAAGG‑AAACCCCGCCGAAGCGGGGtttt                                                                                                                                      <  2:199843/149‑1 (MQ=255)
     gTTCGATGTGCCGCTGGATGCTGATCTAGTGATCGACACCCAGCCCACCAGCGTGGACGAACGCGTCAAACAGCTGCTGGACGTGCTGAGTCAGCGTGGAGCGATCTAAGCGCTACCCAGCAAGGAAAACCACCCTTCCGCGGGGtata                                                                                                                                      >  6:267255/1‑146 (MQ=255)
        cGATGTGCCGCTGGATGCTGATCTTGTGATCGACACCCAGGCCACCAGCGTGGACGAAGGCGTGAAACAGGTGCTGGACGTGCTGCGTCAGCGTGGCGCGATCTAAGCGCTTGCCTGCAAGGAAAACCCCGCTTCGGCGGGGtttttt                                                                                                                                    <  7:122044/148‑1 (MQ=255)
        cGATGTGCCGCTGGATGCTGATCTTGTGATCGACACCCAGGCCACCAGCGTGGACGAAGGAGTGAAACATGTGCTGGACGTGCTGCGTCAGCGTGGCGCGATCTAAGCGCTAGCCTGCAAGGAAAACCCCGCTTCGGCGGGGtttttt                                                                                                                                    <  6:149051/148‑1 (MQ=255)
          aTGTGCCGCTGGATGCTGATCTTGTGATCGACACCCAGGCCACCAGCGTGGACGAAGGCGTGAAACAGGTGCTGGACGTGCTGCGTCAGCGTGGCGCGATCTAAGCGCTAGCCTGCAAGGTAAACCCCGCTTCGGCGGGGtttttt                                                                                                                                    <  2:166132/146‑1 (MQ=255)
          aTGTGCCGCTGGATGCTGATCTTGTGATCGACACCCAGGCCACCAGCGTGGACGAAGGCGTGAAACAGGTGCTGGACGTGCTGCGTCAGCGTGGCGCGATCTAAGCGCTAGCCTGCAAGGGAAACCCCGCTTCGGCGGGGtttttt                                                                                                                                    >  1:166132/1‑146 (MQ=255)
          aTGTGCCGCTGGATGCTGATCTTGTGATCGACACCCAGGCCACCAGCGTGGACGAAGGCGTGAAACAGGTGCTGGACGTGCTGCGTCAGCGTGGCGCGATCTAAGCGCTAGCCTGCAAGGAAAACCCCGCTTCGGCGGGGTTTTTTCTa                                                                                                                                 <  5:52892/149‑1 (MQ=255)
             tgCCGCTGGATGCTGATCTTGTGATCGACACCCAGGCCACCAGCGTGGACGAAGGCGTGAAACAGGTGCTGGACGTGCTGCGTCAGCGTGGCGCGATCTAAGCGCTAGCCTGCAAGGAAAACCCCGCCGAAGCGGGGttttccttgca                                                                                                                               <  5:112579/148‑8 (MQ=255)
               ccGCTGGATGCTGATCTTGTGATCGACACCCAGGCCACCAGCGTGGACGAAGGCGTGAAACAGGTGCTGGACGTGCTGCGTCAGCGTGGCGCGATCTAAGCGCTAGCCTGCAAGGAAAACCCCGCTTCGGCGGGGTTTTTTCTATTGCt                                                                                                                            <  6:145058/149‑1 (MQ=255)
                cGCTGGATGCTGATCTTGTGATCGACACCCAGGCCACCAGCGTGGACGAAGGCGTGAAAGAGGTGCTGGACGTGCTGCGTCAGCGTGGCGCGATCTTAGCGCTAGCCTGCTAGGAAAACCCCGCGTCGGCGGGGGGTTTTGTATTGGTc                                                                                                                           >  8:201576/1‑149 (MQ=255)
                      aTGCTGATCTTGTGATCGACACCCAGGCCACCAGCGTGGACGAAGGCGTGAAACAGGTGCTGGACGTGCTGCGTCAGCGTGGCGCGATCTAAGCGCTAGCCTGCAAGGAAAACCCCGCTTCGGCGGGGTTTTTTCTATTGCTCAGTTCg                                                                                                                     <  7:201576/149‑1 (MQ=255)
                         cTGTTCTTGTGATCGACACCCAGGCCACGAGCGTGGACGAAGGCGTGAAACAGGTGCTGGACGTGCTGCGTCAGGGTGGCGCGATCTAAGCGCTAGCCTGCAAGGAAAACCCCGCTTCGGCGGGGTTTTTTCTATTGCTCAGTTCggg                                                                                                                   <  3:62118/148‑1 (MQ=255)
                          tGATCTTGTGATCGACACCCAGGCCACCAGCGTGGACGAAGGCGTGAAACAGGTGCTGGACGTGCTGCGTCAGCGTGGCGCGATCTAAGCGCTAGCCTGCAAGGAAAACCCCGCTTCGGCGGGGTTTTTTCTATTGCTCAGTTCGGGt                                                                                                                  >  5:223588/1‑148 (MQ=255)
                          tGATCTTGTGATCGACACCCAGGCCACCAGCGTGGACGAAGGCGTGAAACAGGTGCTGGACGTGCTGCGTCAGCGTGGCGCGATCTAAGCGCTAGCCTGCAAGGAAAACCCCGCTTCGGCGGGGTTTTTTCTATTGCTCAGTTCGGGt                                                                                                                  >  8:150024/1‑148 (MQ=255)
                            aTCTTGTGATCGACACCCAGGCCACCAGCGTGGACGAAGGCGTGAAACAGGTGCTGGACGTGCTGCGTCAGCGTGGCGCGATCTAAGCGCTAGCCTGCAAGGAAAACCCCGCTTCGGCGGGGTTTTTTCTATTGCTCAGTTCGGGt                                                                                                                  <  6:223588/146‑1 (MQ=255)
                             tCTTGTGATCGACACCCAGGCCACCAGCGTGGACGAAGGCGTGAAACAGGTGCTGGACGTGCTGCGTCAGCGTGGCGCGATCTAAGCGCTAGCCTGCAAGGAAAACCCCGCCGAAGCGGGGttttccttgc                                                                                                                                >  8:217052/1‑125 (MQ=255)
                                       gACACCCAGGCCACCAGCGTGGACGAAGGCGTGAAACAGGTGCTGGACGTGCTGCGTCAGCGTGGCGCGATCTAAGCGCTAGCCTGCAAGGAAAACCCCGCTTCGGCGGGGTTTTTTCTATTGCTCAGTTCGGGTGAAAAGGCCCTCa                                                                                                     <  7:91612/148‑1 (MQ=255)
                                        acacCCAGGCCACCAGCGTGGACGAAGGCGTGAAACAGGTGCTGGACGTGCTGCGTCAGCGTGGCGCGATCTAAGCGCTAGCCTGCAAGGAAAACCCCGCTTCGGCGGGGTTTTTTCTATTGCTCAGTTCGGGTGAAAAGGCCCTCaga                                                                                                   <  8:74665/149‑1 (MQ=255)
                                            ccAGGCCACCAGCGTGGACGAAGGCGTGAAACAGGTGCTGGACGTGCTGCGTCAGCGTGGCGCGATCTAAGCGCTAGCCTGCAAGGAAAACCCCGCTTCGGCGGGGTTTTTTCTATTGCTCAGTTCgg                                                                                                                    >  8:230934/1‑128 (MQ=255)
                                            ccAGGCCACCAGCGTGGACGAAGGCGTGAAACAGGTGCTGGACGTGCTGCGTCAGCGTGGCGCGATCTAAGCGCTAGCCTGCAAGGAAAACCCCGCTTCGGCGGGGTTTTTTCTATTGCTCAGTTCgg                                                                                                                    <  7:230934/128‑1 (MQ=255)
                                            ccAGGCCACCAGCGTGGACGAAGGCGTGAAACAGGTGCTGGACGTGCTGCGTCAGCGTGGCGCGATCTAAGCGCTAGCCTGCAAGGAAAACCCCGCTTCGGCGGGGTTTTTTCTATTGCTCAGTTCGGGTGAAAAGGCCCTCAGAGtt                                                                                                >  1:258734/1‑148 (MQ=255)
                                            ccAGGCCACCAGCGTGGACGAAGGCGTGAAACAGGTGCTGGACGTGCTGCGTCAGCGTGGCGCGATCTAAGCGCTAGCCTGCAAGGAAAACCCCGCTTCGGCGGGGTTTTTTCTATTGCTCAGTTCGGGTGAAAAGGCCCTCAGAGTTc                                                                                               <  7:150024/149‑1 (MQ=255)
                                                    ccaGCGTGGACGAAGGCGTGAAACAGGTGCTGGACGTGCTGCGTCAGCGTGGCGCGATCTAAGCGCTAGCCTGCAAGGAAAACCCCGCTTCGGCGGGGTTTTTTCTATTGCTCAGTTCGGGt                                                                                                                  <  4:45884/122‑1 (MQ=255)
                                                    ccaGCGTGGACGAAGGCGTGAAACAGGTGCTGGACGTGCTGCGTCAGCGTGGCGCGATCTAAGCGCTAGCCTGCAAGGAAAACCCCGCTTCGGCGGGGTTTTTTCTATTGCTCAGTTCGGGt                                                                                                                  >  3:45884/1‑122 (MQ=255)
                                                    ccaGCGTGGACGAAGGCGTGAAACAGGTGCTGGACGTGCTGCGTCAGCGTGGCGCGATCTAAGCGCTAGCCTGCAAGGAAAACCCCGCTTCGGCGGGGTTTTTTCTATTGCTCAGTTCGGGTGAAAAGGCCCTCAGAGTTCAGGGCCtt                                                                                       <  3:247895/149‑1 (MQ=255)
                                                            gACGAAGGCGTGAAACAGGTGCTGGACGTGCTGCGTCAGCGTGGCGCGATCTAAGCGCTAGCCTGCAAGGAAAACCCCGCTTCGGCGGGGTTTTTTCTATTGCTCAGTTCGGGTGAAAAGGCCCTCAGAGTTCAGGGCCTTGCTCgtgt                                                                               >  7:154276/1‑149 (MQ=255)
                                                                        aaaCAGGTGCTGGACGTGCTGCGTCAGCGTGGCGCGATCTAAGCGCTAGCCTGCAAGGAAAACCCCGCTTCGGCGGGGTTTTTTCTATTGCTCAGTTCGGGTGAAAAGGCCCTc                                                                                                      >  5:204380/1‑114 (MQ=255)
                                                                        aaaCAGGTGCTGGACGTGCTGCGTCAGCGTGGCGCGATCTAAGCGCTAGCCTGCAAGGAAAACCCCGCTTCGGCGGGGTTTTTTCTATTGCTCAGTTCGGGTGAAAAGGCCCTc                                                                                                      <  6:204380/114‑1 (MQ=255)
                                                                         aaCAGGTGCTGGACGTGCTGCGTCAGCGTGGCGCGATCTAAGCGCTAGCCTGCAAGGAAAACCCCGCTTCGGCGGGGTTTTTTCTATTGCTCAGTTCGGGTGAAAAGGCCCTCAGAGTTCAGGGCCTTGCTCGTGTTAGCTGGGCTTg                                                                   >  6:57066/1‑148 (MQ=255)
                                                                            aGGTGCTGGACGTGCTGCGTCAGCGTGGCGCGATCTAAGCGCTAGCCTGCAAGGAAAACCCCGCTTCGGCGGGGTTTTTTCTATTGCTCAGTTCGGGTGAAAAGGCCCTCAGAGTTCAGGGCCTTGc                                                                                     <  2:236251/127‑1 (MQ=255)
                                                                            aGGTGCTGGACGTGCTGCGTCAGCGTGGCGCGATCTAAGCGCTAGCCTGCAAGGAAAACCCCGCTTCGGCGGGGTTTTTTCTATTGCTCAGTTCGGGTGAAAAGGCCCTCAGAGTTCAGGGCCTTGc                                                                                     >  1:236251/1‑127 (MQ=255)
                                                                            aGGTGCTGGACGTGCTGCGTCAGCGTGGCGCGATCTAAGCGCTAGCCTGCAAGGAAAACCCCGCTTCGGCGGGGTTTTTTCTATTGCTCAGTTCGGGTGAAAAGGCCCTCAGAGTTCAGGGCCTTGCTCGTGTTAGCTGGGCTTGAAAc                                                               >  8:215989/1‑149 (MQ=255)
                                                                                        tgctgcGTCAGCGTGGCGCGATCTAAGCGCTAGCCTGCAAGGAAAACCCCGCTTCGGCGGGGTTTTTTCTATTGCTCAGTTCGGGTGAAAAGGCCCTCAGAGTTCAGGGCCTTGCTCGTGTTAGCTGGGCTTGAAACCGTCTTTACCGt                                                   <  2:258734/149‑1 (MQ=255)
                                                                                         gctgcGTCAGCGTGGCGCGATCTAAGCGCTAGCCTGCAAGGAAAACCCCGCTTCGGCGGGGTTTTTTCTATTGCTCAGTTCGGGTGAAAAGGCCCTCAGAGTTCAGGGCCTTGCTCGTGTTAGCTGGGCTTGAAACCGTCTTTACCGtt                                                  >  1:15640/1‑149 (MQ=255)
                                                                                            gcGTCAGCGTGGCGCGATCTAAGCGCTAGCCTGCAAGGAAAACCCCGCTTCGGCGGGGTTTTTTCTATTGCTCAGTTCGGGTGAAAAGGCCCTCAGAGTTCAGGGCCTTGCTCGTGTTAGCTGGGCTTGAAACCGTCTTTACCGTTGGc                                               >  7:76590/1‑149 (MQ=255)
                                                                                             cGTCAGCGTGGCGCGATCTAAGCGCTAGCCTGCAAGGAAAACCCCGCTTCGGCGGGGTTTTTTCTATTGCTCAGTTCGGGTGAAAAGGCCCTCAGAGTTCAGGGCCTTGCTCGTGTTAGCTGGGCTTGAAACCGTCTTTACCGTTGGc                                               >  8:171894/1‑148 (MQ=255)
                                                                                             cGTCAGCGTGGCGCGATCTAAGCGCTAGCCTGCAAGGAAAACCCCGCTTCGGCGGGGTTTTTTCTATTGCTCAGTTCGGGTGAAAAGGCCCTCAGAGTTCAGGGCCTTGCTCGTGTTAGCTGGGCTTGAAACCGTCTTTACCGTTGGc                                               <  8:76590/148‑1 (MQ=255)
                                                                                                        cgcgATCTAAGCGCTAGCCTGCAAGGAAAACCCCGCTTCGGCGGGGTTTTTTCTATTGCTCAGTTCGGGTGAAAAGGCCCTCAGAGTTCAGGGCCTTGCTCGTGTTAGCTGGGCTTGAAACCGTCTTTACCGTTGGCTAGCCGCCATTg                                   >  5:154283/1‑149 (MQ=255)
                                                                                                               tAAGCGCTAGCCTGCAAGGAAAACCCCGCTTCGGCGGGGTTTTTTCTATTGCTCAGTTCGGGTGAAAAGGCCCTCAGAGTTCAGGGCCTTGCTCGTGTTAGCTGGGCTTGAAACCGTCTTTACCGTTGGCTAGCCGCCATTGCTTGAc                             >  7:201764/1‑148 (MQ=255)
                                                                                                                 aGCGCTAGCCTGCAAGGAAAACCCCGCTTCGGCGGGGTTTTTTCTATTGCTCAGTTCGGGTGAAAAGGCCCTCAGAGTTCAGGGCCTTGCTCGTGTTAGCTGGGCTTGAAACCGTCTTTACCGtt                                                  >  4:251069/1‑125 (MQ=255)
                                                                                                                 aGCGCTAGCCTGCAAGGAAAACCCCGCTTCGGCGGGGTTTTTTCTATTGCTCAGTTCGGGTGAAAAGGCCCTCAGAGTTCAGGGCCTTGCTCGTGTTAGCTGGGCTTGAAACCGTCTTTACCGtt                                                  <  3:251069/125‑1 (MQ=255)
                                                                                                                 aGCGCTAGCCTGCAAGGAAAACCCCGCTTCGGCGGGGTTTTTTCTATTGCTCAGTTCGGGTGAAAAGGCCCTCAGAGTTCAGGGCCTTGCTCGTGTTAGCTGGGCTTGAAACCGTCTTTACCGTTGGCTAGCCGCCATTGCtt                                <  3:160110/143‑1 (MQ=255)
                                                                                                                    gcTAGCCTGCAAGGAAAACCCCGCTTCGGCGGGGTTTTTTCTATTGCTCAGTTCGGGTGAAAAGGCCCTCAGAGTTCAGGGCCTTGCTCGTGTTAGCTGGGCTTGAAACCGTCTTTACCGTTGGCTAGCCGCCATTGCTTGACTATTTc                       <  7:171894/149‑1 (MQ=255)
                                                                                                                        gCCTGCAAGGAAAACCCCGCTTCGGCGGGGTTTTTTCTATTGCTCAGTTCGGGTGAAAAGGCCCTCAGAGTTCAGGGCCTTGCTCGTGTTAGCTGGGCTTGAAACCGTCTTTACCGTTGGCTAGCCGCCATTGCTTGACTATTTCTa                     <  5:57066/147‑1 (MQ=255)
                                                                                                                              aaGGAAAACCCCGCTTCGGCGGGGTTTTTTCTATTGCTCAGTTCGGGTGAAAAGGCCCTCAGAGTTCAGGGCCTTGCTCGTGTTAGCTGGGCTTGAAACCGTCTTTACCGTTGGCTAGCCGCCATTGCTTGACTATTTCTAGTACtttt             >  1:94745/1‑149 (MQ=255)
                                                                                                                                 aaaaaCCCCGCCGAAGCGGGGTTTTTTCTATTGCTCAGTTCGGGTGAAAAGGCCCTCAGAGTTCAGGGCCTTGCTCGTGTTAGCTGGGCTTGAAACCGTCTTTACCGTTGGCTAGCCGCCATTGCTTGACTATTTCTAGTACTTTTTCa          >  2:241065/2‑149 (MQ=255)
                                                                                                                                    acccccGCTTCGGCGGGGTTTTTTCTATTGCTCAGTTCGGGTGAAAAGGCCCTCAGAGTTCAGGGCCTTGCTCGTGTTAGCTGGGCTTGAAACCGTCTTTACCGTTGGCTAGCCGCCATTGCTTGACTATTTCTAGTACTTTTTCAGGt       <  8:120854/147‑1 (MQ=255)
                                                                                                                                      ccccGCTTCGGCGGGGTTTTTTCTATTGCTCAGTTCGGGTGAAAAGGCCCTCAGAGTTCAGGGCCTTGCTCGTGTTAGCTGGGCTTGAAACCGTCTTTACCGTTGGCTAGCCGCCATTGCTTGACTATTTCTAGTACTTTTTCAGGtt      >  4:233106/1‑148 (MQ=255)
                                                                                                                                        ccGCTTCGGCGGGGTTTTTTCTATTGCTCAGTTCGGGTGAAAAGGCCCTCAGAGTTCAGGGCCTTGCTCGTGTTAGCTGGGCTTGAAACCGTCTTTACCGTTGGCTAGCCGCCATTGCTTGACTATTTCTAGTACTTTTTCAGGTTTAc   >  2:102029/1‑149 (MQ=255)
                                                                                                                                         cGCTTCGGCGGGGTTTTTTCTATTGCTCAGTTCGGGTGAAAAGGCCCTCAGAGTTCAGGGCCTTGCTCGTGTTAGCTGGGCTTGAAACCGTCTTTACCGTTGGCTAGCCGCCATTGCTTGACTATTTCTAGTACTTTTTCAGGTTTAcc  <  6:154283/149‑1 (MQ=255)
                                                                                                                                          gCTTCGGCGGGGTTTTTTCTATTGCTCAGTTCGGGTGAAAAGGCCCTCAGAGTTCAGGGCCTTGCTCGTGTTAGCTGGGCTTGAAACCGTCTTTACCGTTGGCTAGCCGCCATTGCTTGACTATTTCt                      <  4:6364/128‑1 (MQ=255)
                                                                                                                                          gCTTCGGCGGGGTTTTTTCTATTGCTCAGTTCGGGTGAAAAGGCCCTCAGAGTTCAGGGCCTTGCTCGTGTTAGCTGGGCTTGAAACCGTCTTTACCGTTGGCTAGCCGCCATTGCTTGACTATTTCt                      >  3:6364/1‑128 (MQ=255)
                                                                                                                                            |                                                                                                                                                 
TTCCCGTTCGATGTGCCGCTGGATGCTGATCTTGTGATCGACACCCAGGCCACCAGCGTGGACGAAGGCGTGAAACAGGTGCTGGACGTGCTGCGTCAGCGTGGCGCGATCTAAGCGCTAGCCTGCAAGGAAAACCCCGCTTCGGCGGGGTTTTTTCTATTGCTCAGTTCGGGTGAAAAGGCCCTCAGAGTTCAGGGCCTTGCTCGTGTTAGCTGGGCTTGAAACCGTCTTTACCGTTGGCTAGCCGCCATTGCTTGACTATTTCTAGTACTTTTTCAGGTTTACC  >  NC_002947/1492126‑1492411

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑