| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,508,079 | G→T | 17.7% | intergenic (+18/+44) | sspB → / ← PP_1322 | ClpXP protease specificity‑enhancing factor/lipoprotein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,508,079 | 0 | G | T | 17.7% | 117.9 / 18.4 | 51 | intergenic (+18/+44) | sspB/PP_1322 | ClpXP protease specificity‑enhancing factor/lipoprotein |
| Reads supporting (aligned to +/- strand): ref base G (15/27); new base T (3/6); total (18/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.78e-01 | |||||||||||
GAGCCGTCTTCGATGGATGGCGACGAGCTGGAAGACGATGATGTGCAGCCGGATGACGATGGCCCGCCAGACGATGGAGGGCAGCCGCCGCGTCCGACTGGCCGGCCAAGCCTGAAAGTGGTCAAGTAACAAAAAAAGGCGA‑TCCGGATGGATCGCC‑TTTTTTGTTTGGCCTGTCCAGGCAATCACTGAGTCAATCGATGTACTCGAACAGCTTGACGATCTTCTGCACGCCCGAAACACCCTGCACCACGGCAGTGGCCGAGTTGGCTTCTTGACGGGTGACCAGGCCC > NC_002947/1507933‑1508222 | gAGCCGTCTTCGATGGATGGCGACGAGCTGGAAGACGATGATGTGCAGCCGTATGACGATGGCCCGCCAGACTATGGAGGGCAGCCGCCGCGTCCGACTGGCCGGCCAAGCCTGAAAGTGGTCAATTAACAAAAAAAGGCGA‑TCCgga < 2:240667/148‑1 (MQ=255)gAGCCGTCTTCGATGGATGGCGACGAGCTGGAAGACGATGATGTGCAGCCGGATGACGATGGCCCGCCAGACGATGGAGGGCAGCCGCCGCGTCCGACTGGCCGGCCAAGCCTGAAAGTGGTCAAGTAACAAAAAAAGGCGA‑TCCggat < 7:107520/149‑1 (MQ=255)gAGCCGTCTTAGATGGATGGCGACGAGCTGGAAGACGATGATGTGCAGCCGGATGACGATGGCCCGCCAGACGATGGACGGCAGCCGCCGCGTCCGACTGGCCGGCCAAGCCTGAAAGTGGTCAAGTAACAAAAAAAGGCGA‑TCCggat > 6:20719/1‑149 (MQ=255) cGTCTTCGATGGATGGCGACGAGCTGGAAGACGATGATGTGCAGCCGGATGACGATGGCCCGCCAGACGATGGAGGGCAGCCGCCGCGTCCGACTGGCCGGCCAAGCCTGAAAGTGGTCAAGTAACAAAAAAAGGCGA‑TCCggatggat > 6:160464/1‑149 (MQ=255) cGTCTTCGATGGATGGCGACGAGCTGGAAGACGATGATGTGCAGCCGGATGACGATGGCCCGCCAGACGATGGAGGGCAGCCGCCGCGTCCGACTGGCCGGCCAAGCCTGAAAGTGGTCAAGTAACAAAAAAAGGCGA‑TCCggatggat < 2:101980/149‑1 (MQ=255) ttCGATGGATGGCGACGAGCTGGAAGACGATGATGTGCAGCCGGATGACGATGGCCCGCCAGACGATGGAGGGCAGCCGCCGCGTCCGACTGGCCGGCCAAGCCTGAAAGTGGTCAAGTAACAAAAAAAGGCGA‑TCCGGATGGATCGcc > 7:66260/1‑149 (MQ=255) cGATGGATGGCGACGAGCTGGAAGACGATGATGTGCAGCCGGATGACGATGGCCCGCCAGACGATGGAGGGCAGCCGCCGCGTCCGACTGGCCGGCCAAGCCTGAAAGTGGTCAAGTAAC‑AAAAAAGGCGA‑TCCATCCGGATCGCC‑t < 4:22221/147‑1 (MQ=255) gatggatgGCGACGAGCTGGAAGACGATGATGTGCAGCCGGATGACGATGGCCCGCCAGACGATGGAGGGCAGCCGCCGCGTCCGACTGGCCGGCCAAGCCTGAAAGTGGTCAAGTAACAAAAAAAGGCGA‑TCCGGATGGATCGCC‑tt < 1:94103/148‑1 (MQ=255) gatggatgGCGACGAGCTGGAAGACGATGATGTGCAGCCGGATGACGATGGCCCGCCAGACGATGGAGGGCAGCCGCCGCGTCCGACTGGCCGGCCAAGCCTGAAAGTGGTCAAGTAACAAAAAAAGGCGA‑TCCATCCGGATCGCC‑tt < 5:214727/148‑1 (MQ=255) atggatggCGACGAGCTGGAAGACGATGATGTGCAGCCGGATGACGATGGCCCGCCAGACGATGGAGGGCAGCCGCCGCGTCCGACTGGCCGGCCAAGCCTGAAAGTGGTCAAGTAACAAAAAAAGGCGA‑TCCGGATGGATCGCC‑tttt < 6:173472/149‑1 (MQ=255) tggatggCGACGAGCTGGAAGACGATGATGTGCAGCCGGATGACGATGGCCCGCCAGACGATGGAGGGCAGCCGCCGCGTCCGACTGGCCGGCCAAGCCTGAAAGTGGTCAAGTAACAAAAAAAGGCGA‑TCCGGATGGATCGCC‑ttttt < 4:239199/149‑1 (MQ=255) catcgaagacgaGCTGGAAGACGATGATGTGCAGCCGGATGACGATGGCCCGCCAGACGATGGAGGGCAGCCGCCGCGTCCGACTGGCCGGCCAAGCCTGAAAGTGGTCAAGTAACAAAAAAAGGCGA‑TCCGGATGGATCGCC‑tttttt < 6:148480/142‑1 (MQ=255) gatggCGACGAGCTGGAAGACGATGATGTGCAGCCGGATGACGATGGCCCGCCAGACGATGGAGGGCAGCCGCCGCGTCCGACTGGCCGGCCAAGCCTGAAAGTGGTCAAGTAACAAAAAAAGGCGA‑TCCATCCGGATCGCC‑ttttttt < 4:14807/149‑2 (MQ=255) gCGACGAGCTGGAAGACGATGATGTTCAGCCGTATGACGATGGGCCGCCAGACGATGGAGGGCAGCCGCCGCGTACGACTGGCCGGCCAAGCCTGAAAGTGGTCAAGTAAC‑AAAAAAGGCGA‑TCCATCCGGATCGCC‑tttttttgttac < 7:6405/149‑7 (MQ=37) gCGACGAGCTGGAAGACGATGATGTGCAGCCGGATGACGATGGCCCGCCAGACGATGGAGGGCAGCCGCCGCGTCCGACTGGCCGGCCAAGCCTGAAAGTGGTCAAGTAACAAAAAAAGGCGA‑TCCGGATGGATCGCC‑TTTtttgttt < 1:632/148‑1 (MQ=255) cgacgaGCTGGAAGACGATGATGTGCAGCCGGATGACGATGGCCCGCCAGACGATGGAGGGCAGCCGCCGCGTCCGACTGGCCGGCCAAGCCTGAAAGTGGTCAAGTAACAAAAAAAGGCGA‑TCCGGATGGATCGCC‑TTTTTTGTTTgg > 4:63950/1‑149 (MQ=255) cgacgaGCTGGAAGACGATGATGTGCAGCCGGATGACGATGGCCCGCCAGACGATGGAGGGCAGCCGCCGCGTCCGACTGGCCGGCCAAGCCTGAAAGTGGTCAAGTAACAAAAAAAGGCGA‑TCCGGATGGATCGCC‑TTTTTTGTTTgg < 5:102664/149‑1 (MQ=255) cgacgaGCTGGAAGACGATGATGTGCAGCCGGATGACGATGGCCCGCCAGACGATGGAGGGCAGCCGCCGCGTCCGACTGGCCGGCCAAGCCTGAAAGTGGTCAAGTAACAAAAAAAGGCGA‑TCCGGATGGATCGCC‑TTTTTTGTTTgg < 5:160464/149‑1 (MQ=255) gacgaGCTGGAAGACGATGATGTGCAGCCGGATGACGATGGCCCGCCAGACGATGGAGGGCAGCCGCCGCGTCCGACTGGCCGGCCAATCCTGAAAGTGGTCAAGTAACAAAAAAAGGCGA‑TCCGGATGGATCGCC‑TTTTTTGTTTGGc < 6:5177/149‑1 (MQ=255) tGGAAGACGATGATGTGCAGCCGGATGACGATGGCCCGCCAGACGATGGAGGGCAGCCGCCGCGTCCGACTGGCCGGCCAAGCCTGAAAGTGGTCAAGTAACAAAAAAAGGCGA‑TCAGGATGGATCGCC‑TTTTTTGTTTGGCCTGTc > 6:79163/1‑147 (MQ=255) gAAGACGATGATGTGCAGCCGGATGACGATGGCCCGCCAGACGATGGAGGGCAGCCGCCGCGTCCGACTGGCCGGCCAAGCCTGAAAGTGGTCAAGTAACAAAAAAAGGCGA‑TCCGGATGGATCGCC‑TTTTTTGTTTGGCCTGTCCAgg > 8:246990/1‑149 (MQ=255) tgatgTGCAGCCGGATGACGATGGCCCGCCAGACGATGGAGGGCAGCCGCCGCGTCCGACTGGCCGGCCAAGCCTGAAAGTGGTCAAGTAACAAAAAAAGGCGA‑TCCGGATGGATCGCC‑TTTTTTGTTTGGCCTGTCCAGGCAATCACt < 5:20719/149‑1 (MQ=255) atgTGCAGCCGGATGACGATGGCCCGCCAGACGATGGAGGGCAGCCGCCGCGTCCGACTGGCCGGCCAAGCCTGAAAGTGGTCAAGTAACAAAAAAAGGCGACTCCGGATGGATCGCC‑TTTTTTGTTTGGCCTGTCCAGGCAATCACt < 1:192105/148‑1 (MQ=255) gCAGCCGGATGACGATGGCCCGCCAGACGATGGAGGGCAGCCGCCGCGTCCGACTGGCCGGCCAAGCCTGAAAGTGGTCAAGTAACAAAAAAAGGCGA‑TCCGGATGGATCGCC‑TTTTTTGTTTGGCCTGTCCAGGCAATCACTGAGTc > 7:97313/1‑148 (MQ=255) cAGCCGGATGACGATGGCCCGCCAGACGATGGAGGGCAGCCGCCGCGTCCGACTGGCCGGCCAAGCCTGAAAGTGGTCAAGTAACAAAAAAAGGCGA‑TCCGGATGGATCGCC‑TTTTTTGTTTGGTCTGTCCAGGCAATCACTGAGTCaa > 2:179867/1‑149 (MQ=255) aGCCGGATGACGATGGCCCGCCAGACGATGGAGGGCAGCCGCCGCGTCCGACTGGCCGGCCAAGCCTGAAAGTGGTCAAGTAACAAAAAAAGGCGA‑TCCGGATGGATCGCC‑TTTTTTGTTTGGCCTGTCCAGGCAATCACTGAGTCAAt < 2:208632/149‑1 (MQ=255) gCCGGATGACGATGGCCCGCCAGACGATGGAGGGCAGCCGCCGCGTCCGACTGGCCGGCCAAGCCTGAAAGTGGTCAAGTAACAAAAAAAGGCGA‑TCCGGATGGATCGCC‑TTTTTTGTTTGGCCTGTCCAGGCAATCACTGAGTCAATc < 2:135596/149‑1 (MQ=255) ggATGACGATGGCCCGCCAGACGATGGAGGGCAGCCGCCGCGTCCGACTGGCCGGCCAAGCCTGAAAGTGGTCAAGTAACAAAAAAAGGCGA‑TCCGGATGGATCGCC‑TTTTTTGTTTGGCCTGTCCAGGCAATCACTGAGTCAATCGAt < 5:160631/149‑1 (MQ=255) ggATGACGATGGCCCGCCAGACGATGGAGGGCAGCCGCCGCGTCCGACTGGCCGGCCAAGCCTGAAAGTGGTCAAGTAACAAAAAAAGGCGA‑TCCGGATGGATCGCC‑TTTTTTGTTTGGCCTGTCCAGGCAATCACTGAGTCAATCGAt < 7:246990/149‑1 (MQ=255) cGATGGCCCGCCAGACGATGGAGGGCAGCCGCCGCGTCCGACTGGCCGGCCAAGCCTGAAAGTGGTCAAGTAACAAAAAAAGGCGA‑TCCGGATGGATCGCC‑TTTTTTGTTTGGCCTGTCCAGGCAATCACTGAGTCAATCGATGTACTc < 1:28705/149‑1 (MQ=255) cGATGGCCCGCCAGACGATGGAGGGCAGCCGCCGCGTCCGACTGGCCGGCCAAGCCTGAAAGTGGTCAAGTAACAAAAAAAGGCGA‑TCCGGATGGATCGCC‑TTTTTTGTTTGGCCTGTCCAGGCAATCACTGAGTCAATCGATGTACTc > 2:28705/1‑149 (MQ=255) ccAGACGATGGAGGGCAGCCGCCGCGTCCGACTGGCCGGCCAAGCCTGAAAGTGGTCAAGTAACAAAAAAAGGCGA‑TCCGGATGGATCGCC‑TTTTTTGTTTGGCCTGTCCAGGCAATCACTGAGTCAATCGATGTACTCGAACAGCTTg < 3:63950/149‑1 (MQ=255) gATGGAGGGCAGCCGCCGCGTCCGACTGGCCGGCCAAGCCTGAAAGTGGTCAAGTAACAAAAAAAGGCGA‑TCCGGATGGATCGCC‑TTTTTTGTTTGGc > 8:67065/1‑98 (MQ=255) gATGGAGGGCAGCCGCCGCGTCCGACTGGCCGGCCAAGCCTGAAAGTGGTCAAGTAACAAAAAAAGGCGA‑TCCGGATGGATCGCC‑TTTTTTGTTTGGc < 7:67065/98‑1 (MQ=255) tCCGACTGGCCGGCCAAGCCTGAAAGTGGTCAAGTAACAAAAAAAGGCGA‑TCCGGATGGATCGCC‑TTTTTTGTTTGGTCTGTCCAGGCAATCACTGAGTCAATCGATGTACTCGAACAGCTTGACGATCTTCTGCACGCCCGAAACAcc < 1:179867/149‑1 (MQ=255) tCCGACTGGCCGGCCAAGCCTGAAAGTGGTCAAGTAACAAAAAAAGGCGA‑TCCGGATGGATCGCC‑TTTTTTGTTTGGCCTGTCCAGGCAATCACTGAGTCAATCGATGTACTCGAACAGCTTGACGATCTTCTGCACGCCCGAAACAcc < 8:66260/149‑1 (MQ=255) aCTGGCCGGCCAAGCCTGAAAGTGGTCAAGTAACAAAAAAAGGCGA‑TCCGGATGGATCGCC‑TTTTTTGTTTGGCCTGTCCAGGCAATCACTGAGTCAATCGATGTACTCGAACAGCTTGACGATCTTCTGCACGCCCGAAACACCCTg > 2:86397/1‑148 (MQ=255) tGGCCGGCCAAGCCTGAAAGTGGTCAAGTAACAAAAAAAGGCGA‑TCCGGATGGATCGCC‑TTTTTTGTTTGGCCTGTCCAGGCAATCACTGAGTCAATCGATGTACTCGAACAGCTTGACGATCTTCTGCACGCCCGAAACACCCTGcac > 7:121405/1‑149 (MQ=255) ggccggccAAGCCTGAAAGTGGTCAAGTAACAAAAAAAGGCGA‑TCCGGATGGATCGCC‑TTTTTTGTTTGGCCTGTCCAGGCAATCACTGAGTCAATCGATGTACTCGAACAGCTTGACGATCTTCTg < 1:103340/127‑1 (MQ=255) ggccggccAAGCCTGAAAGTGGTCAAGTAACAAAAAAAGGCGA‑TCCGGATGGATCGCC‑TTTTTTGTTTGGCCTGTCCAGGCAATCACTGAGTCAATCGATGTACTCGAACAGCTTGACGATCTTCTg > 2:103340/1‑127 (MQ=255) ggccAAGCCTGAAAGTGGTCAAGTAACAAAAAAAGGCGA‑TCCGGATGGATCGCC‑TTTTTTGTTTGGCCTGTCCAGGCAATCACTGAGTCAATCGATGTACTCGAACAGCTTGACGATCTTCTGCACGCCCGAAACAccc < 5:73085/139‑1 (MQ=255) ggccAAGCCTGAAAGTGGTCAAGTAACAAAAAAAGGCGA‑TCCGGATGGATCGCC‑TTTTTTGTTTGGCCTGTCCAGGCAATCACTGAGTCAATCGATGTACTCGAACAGCTTGACGATCTTCTGCACGCCCGAAACAccc > 6:73085/1‑139 (MQ=255) aGTAACAAAAAAAGGCGA‑TCCGGATGGATCGCC‑TTTTTTGTTTGGCCTGTCCAGGCAATCACTGAGTCAATCGATGTACTCGAACAGCTTGACGATCTTCTGCACGCCCGAAACACCCTGCACCACGGCAGTGGCCGAGTTGGcttctt < 1:86397/149‑1 (MQ=255) aaaaaaaGGCGA‑TCCGGATGGATCGCC‑TTTTTTGTTTGGCCTGTCCAGGCAATCACTGAGTCAATCGATGTACTCGAACAGCTTGACGATCTTCTGCACGCCCGAAACACCCTGCACCACGGCAGTGGCCGAGTTGGCTTCTTGACg > 5:34811/1‑147 (MQ=255) aaaaaaaGGCGA‑TCCGGATGGATCGCC‑TTTTTTGTTTGGCCTGTCCAGGCAATCACTGAGTCAATCGATGTACTCGAACAGCTTGACGATCTTCTGCACGCCCGAAACACCCTGCACCACGGCAGTGGCCGAGTTGGCTTCTTGACg < 6:34811/147‑1 (MQ=255) aaaaaaGGCGA‑TCCATCCGGATCGCC‑TTTTTTGTTTGGCCTGTCCAGGCAATCACTGAGTCAATCGATGTACTCGAACAGCTTGACGATCTTCTGCACGCCCGAAACACCCTGCACCACGGCAGTGGCCGAGTTGGCTTCTTGAc > 6:103008/1‑145 (MQ=255) aaaaaaGGCGA‑TCCATCCGGATCGCC‑TTTTTTGTTTGGCCTGTCCAGGCAATCACTGAGTCAATCGATGTACTCGAACAGCTTGACGATCTTCTGCACGCCCGAAACACCCTGCACCACGGCAGTGGCCGAGTTGGCTTCTTGAc < 5:103008/145‑1 (MQ=255) aaaaaaGGCGA‑TCCATCCGGATCGCC‑TTTTTTGTTTGGCCTGTCCAGGCAATCACTGAGTCAATCGATGTACTCGAACAGCTTGACGATCTTCTGCACGCCCGAAACACCCTGCACCACGGCAGTGGCCGAGTTGGCTTCTTGAc > 2:93787/1‑145 (MQ=255) aaaaaaGGCGA‑TCCATCCGGATCGCC‑TTTTTTGTTTGGCCTGTCCAGGCAATCACTGAGTCAATCGATGTACTCGAACAGCTTGACGATCTTCTGCACGCCCGAAACACCCTGCACCACGGCAGTGGCCGAGTTGGCTTCTTGAc < 1:93787/145‑1 (MQ=255) aaaaaGGCGA‑TCCGGATGGATCGCC‑TTTTTTGTTTGGCCTGTCCAGGCAATCACTGAGTCAATCGATGTACTCGAACAGCTTGACGATCTTCTGCACGCCCGAAACACCCTGCACCACGGCAGTGGCCGAGTTGGCTTCTTGACGGGTg > 6:35631/1‑149 (MQ=255) aaaaGGCGA‑TCCGGATGGATCGCC‑TTTTTTGTTTGGCCTGTCCAGGCAATCACTGAGTCAATCGATGTACTCGAACAGCTTGACGATCTTCTGCACGCCCGAAACACCCt < 7:31931/110‑1 (MQ=255) aaaaGGCGA‑TCCGGATGGATCGCC‑TTTTTTGTTTGGCCTGTCCAGGCAATCACTGAGTCAATCGATGTACTCGAACAGCTTGACGATCTTCTGCACGCCCGAAACACCCt > 8:31931/1‑110 (MQ=255) aaGGCGA‑TCCGGATGTATCGCC‑TTTTTTGTTTGGCCTGTCCAGGCAATCACTGAGTCAATCGATGTACTCGAACAGCTTGACGATCTTCTGCACGCCCGAAACACCCTGCACCACGGCAGTGGCCGAGTTGGCTTCTTGACGGGTGAcc < 5:79163/149‑1 (MQ=255) aaGGCGA‑TCCATCCGGATCGCCTTTTTTTGTTTGGCCTGTCCAGGCAATCACTGAGTCAATCGATGTACTCGAACAGCTTGACGATCTTCTGCACGCCCGAAACACCCTGCACCACGGCAGTGGCCGAGTTGGCTTCTTGACGGGTGAc > 6:272285/1‑149 (MQ=255) ggCGA‑TCCGGATGGATCGCC‑TTTTTTGTTTGGCCTGTCCAGGCAATCACTGAGTCAATCGATGTACTCGAACAGCTTGACGATCTTCTGCACGCCCGAAACACCCTGCACCACGGCAGTGGCCGAGTTGGCTTCTTGACGGGTGACCAg < 8:121405/149‑1 (MQ=255) a‑TCCGGATGGATCGCC‑TTTTTTGTTTGGCCTGTCCAGGCAATCACTGAGTCAATCGATGTACTCGAACAGCTTGACGATCTTCTGCACGCCCGAAACACCCTGCACCACGGCAGTGGCCGAGTTGGCTTCTTGACGGGTGACCAGGccc < 8:97313/149‑1 (MQ=255) | GAGCCGTCTTCGATGGATGGCGACGAGCTGGAAGACGATGATGTGCAGCCGGATGACGATGGCCCGCCAGACGATGGAGGGCAGCCGCCGCGTCCGACTGGCCGGCCAAGCCTGAAAGTGGTCAAGTAACAAAAAAAGGCGA‑TCCGGATGGATCGCC‑TTTTTTGTTTGGCCTGTCCAGGCAATCACTGAGTCAATCGATGTACTCGAACAGCTTGACGATCTTCTGCACGCCCGAAACACCCTGCACCACGGCAGTGGCCGAGTTGGCTTCTTGACGGGTGACCAGGCCC > NC_002947/1507933‑1508222 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |