Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,534,879	G→A	9.2%	intergenic (+70/‑113)	secA → / → argJ	protein translocase subunit SecA/glutamate N‑acetyltransferase/amino acid acetyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,534,879	0	G	A	9.2%	153.4 / 4.8	54	intergenic (+70/‑113)	secA/argJ	protein translocase subunit SecA/glutamate N‑acetyltransferase/amino acid acetyltransferase
Reads supporting (aligned to +/- strand):  ref base G (28/21);  new base A (2/3);  total (30/24)
Fisher's exact test for biased strand distribution p-value = 6.46e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.35e-01

GAAGCTGGGCCGCAACGAGCCGTGCTGGTGTGGTTCGGGCAAGAAGTTCAAGCATTGCCACGGTCAGATCGAGTGATCTGAACCTTACGTTGTACAAGCCTGGGGTCGCTTTGCGGCCCTTTCGCAACACAAGGCCGCTCCCACAGGTACCGCGCTCGTCCATGAACGGGCGCGATCCTTGTGGGGGCGGCCTTGTGTCGCGATGGGGTGCGAAGCGGCCCCTTTTTCGACATCATCGTTTTTTCTAGGAGCGCTCTAATGGCTGTTGGTCTTGGTCCTTTGCCCACCCTGCAC  >  NC_002947/1534734‑1535027                                                                                                                                                  |                                                                                                                                                     gAAGCTGGGCCGCAACGAGCCGTGCTGGTGTGGTTCGGGCAAGAAGTTCAAGCATTGCCACGGTCAGATCGAGTGATCTGAACCTTACGTTGTACAAGCCTGGGGTCGCTTTGCGGCCCTTTCGCAACACAAGGCCGCTCCCACAGGTa                                                                                                                                                   >  6:40515/1‑149 (MQ=255)        ggCCGCAACGAGCCGTGCTGGTGTGGTTCGGGCAAGAAGTTCAAGCATTGCCACGGTCAGATCGAGTGATCTGAACCTTACGTTGTACAAGCCTGGGGTCGCTTTGCGGCCCTTTCGCAACACAAGGCCGCTCCCACAGGTACCGCGCt                                                                                                                                            >  4:85408/1‑149 (MQ=255)         gCCGCAACGATCCGTGCTGGTGTGGTTCGGGCAAGAAGTTCAAGCATTGCCACGGTCAGATCGAGTGATCTGAACCTTACGTTGTACAAGCCTGGGGTCGCTTTGCGGCCCTTTCGCAACACAAGGCCGCTCCCACAGGTACCGCGCTc                                                                                                                                           <  2:77432/149‑1 (MQ=255)          ccGCAACGAGCCGTGCTGGTGTGGTTCGGGCAAGAAGTTCAAGCATTGCCACGGTCAGATCGAGTGATCTGAACCTTACGTTGTACAAGCCTGGGGTCGCTTTGCGGCCCTTTCGCAACACAAGGCCGCTCCCACAGGTACCGCGCTCg                                                                                                                                          >  8:221345/1‑149 (MQ=255)               aCGAGCCGTGCTGGTGTGGTTCGGGCAAGAAGTTCAAGCATTGCCACGGTCAGATCGAGTGATCTGAACCTTACGTTGTACAAGCCTGGGGTCGCTTTGCGGCCCTTTCGCAACACAAGGCCGCTCCCACAGGTACCGCGCTCGTCCAt                                                                                                                                     <  5:88586/149‑1 (MQ=255)                       tGCTGGTGTGGTTCTGGCAAGAAGTTCAAGCATTGCCACGGTCAGATCGAGTGATCTGAACCTTACGTTGTACAAGCCTGGGGGCGCTTCGC‑ACCCCATCGCGACACAAGGCCGCCCCCACAAGGATCGCGCCCGTTCATGGACGAgcg                                                                                                                            <  5:71276/149‑1 (MQ=17)                       tGCTGGTGTGGTTCGGGCAAGAAGTTCAAGCATTGCCACGGTCAGATCGAGTGATCTGAACCTTACGTTGTACAAGCCTGGGGTCGCTTCGC‑ACCCCATCGCGACACAAGGCCGCCCCCACAAGGATCGCGCCCGTTCATGGACGAgcg                                                                                                                            <  5:74994/149‑1 (MQ=18)                             tgtgGTTCGGGCAAGAAGTTCAAGCATTGCCACGGTCAGATCGAGTGATCTGAACCTTACGTTGTACAAGCCTGGGGTCGCTTTGCGGCCCTTTCGCAACACAAGGCCGCTCCCACAGGTACCGCGCTCGTCCATGAACGGGCGCGATc                                                                                                                       >  2:208306/1‑149 (MQ=255)                                 gTTCGGGCAAGAAGTTCAAGCATTGCCACGGTCAGATCGAGTGATCTGAACCTTACGTTGTACAAGCCTGGGGTCGCTTTGCGGCCCTTTCGCAACACAAGGCCGCTCCCACAGGTACCGCGCTCGTCCATGAACGGGCGCGATcc                                                                                                                      >  3:58608/1‑146 (MQ=255)                                 gTTCGGGCAAGAAGTTCAAGCATTGCCACGGTCAGATCGAGTGATCTGAACCTTACGTTGTACAAGCCTGGGGTCGCTTTGCGGCCCTTTCGCAACACAAGGCCGCTCCCACAGGTACCGCGCTCGTCCATGAACGGGCGCGATcc                                                                                                                      <  4:58608/146‑1 (MQ=255)                                 gTTCGGGCAAGAAGTTCAAGCATTGCCACGGTCAGATCGAGTGATCTGAACCTTACGTTGTACAAGCCTGGGGTCGCTTTGCGGCCCTTTCGCAACACAAGGCCGCTCCCACAGGTACCGCGCTCGTCCATGAACGGGCGCGATCCTtg                                                                                                                   >  5:248026/1‑149 (MQ=255)                                 gTTCGGGCAAGAAGTTCAAGCATTGCCACGGTCAGATCGAGTGATCTGAACCTTACGTTGTACAAGCCTGGGGTCGCTTTGCGGCCCTTTCGCAACACAAGGCCGCTCCCACAGGTACCGCGCTCGTCCATGAACGGGCGCGATCCTtg                                                                                                                   >  4:191960/1‑149 (MQ=255)                                 gTTCGGGCAAGAAGTTCAAGCATTGCCACGGTCAGATCGAGTGATCTGAACCTTACGTTGTACAAGCCTGGGGTCGCTTTGCGGCCCTTTCGCAACACAAGGCCGCCCCCACAAGGATCGCGCCCGTTCATGGACGAGcgcggtacctg                                                                                                                   <  2:259415/149‑8 (MQ=25)                                    cGGGCAAGAAGTTCAAGCATTGCCACGGTCAGATCGAGTGATCTGAACCTTACGTTGTACAAGCCTGGGGTCGCTTTGCGGCCCTTTCGCAACACAAGGCCGCTCCCACAGGTACCGCGCTCGTCCATGAACGGGCGCGATCCTtgtg                                                                                                                 <  6:232851/148‑1 (MQ=255)                                     gggCAAGAAGTTCAAGCATTGCCACGGTCAGATCGAGTGATCTGAACCTTACGTTGTACAAGCCTGAGGTCGCTTTGCGGCCCTTTCGCAACACAAGGCCGCTCCCACAGGTACCGCGCTCGTCCATGAACGGGCGCGATCCTTGTgg                                                                                                                <  5:108161/148‑1 (MQ=255)                                        cAAGAAGTTCAAGCATTGCCACGGTCAGATCGAGTGATCTGAACCTTACGTTGTACAAGCCTGGGGTCGCTTTGCGGCCCTTTCGCAACACAAGGCCGCTCCCACAGGTACCGCGCTCGTCCATGAACGGGCGCGATCCTTGTGGGggc                                                                                                            >  5:53106/1‑149 (MQ=255)                                           gaagTTCAAGCATTGCCACGGTCAGATCGAGTGATCTGAACCTTACGTTGTACAAGCCTGGGGTCGCTTTGCGGCCCTTTCGCAACACAAGGCCGCTCCCACAGGTAcc                                                                                                                                                 >  4:29282/1‑109 (MQ=255)                                           gaagTTCAAGCATTGCCACGGTCAGATCGAGTGATCTGAACCTTACGTTGTACAAGCCTGGGGTCGCTTTGCGGCCCTTTCGCAACACAAGGCCGCTCCCACAGGTAcc                                                                                                                                                 <  3:29282/109‑1 (MQ=255)                                             agTTCAAGCATTGCCACGGTCAGATCGAGTGATCTGAACCTTACGTTGTACAAGCCTGGGGTCGCTTTGCGGCCCTTTCGCAACACAAGGCCGCTCCCACAGGTACCGCGCTCGTCCATGAACGGGCGCGATCCTTGTGGGGGCGGcc                                                                                                        <  6:53106/148‑1 (MQ=255)                                              gTTCAAGCATTGCCACGGTCAGATCGAGTGATCTGAACCTTACGTTGTACAAGCCTGGGGTCGCTTTGCGGCCCTTTCGCAACACAAGGCCGCTCCCACAGGTACCGCGCTCGTCCATGAACGGGCGCGATCCTTGTGGGGGCGGCCtt                                                                                                      <  7:221345/149‑1 (MQ=255)                                               ttCAAGCATTGCCACGGTCAGATCGAGTGATCTGAACCTTACGTTGTACAAGCCTGGGGTCGCTTTGCGGCCCTTTCGCAACACAAGGCCGCTCCCACAGGTACCGCGCTCGTCCATGAACGGGCGCGATCCTTGTGGGGGCGGCCTtg                                                                                                     >  5:77243/1‑149 (MQ=255)                                               ttCAAGCATTGCCACGGTCAGATCGAGTGATCTGAACCTTACGTTGTACAAGCCTGGGGTCGCTTTGCGGCCCTTTCGCAACACAAGGCCGCTCCCACAGGTACCGCGCCCGTTCATGGACGAGcgcgg                                                                                                                         >  2:208159/1‑128 (MQ=38)                                               ttCAAGCATTGCCACGGTCAGATCGAGTGATCTGAACCTTACGTTGTACAAGCCTGGGGTCGCTTTGCGGCCCTTTCGCAACACAAGGCCGCTCCCACAGGTACCGCGCCCGTTCATGGACGAGcgcgg                                                                                                                         <  1:208159/129‑2 (MQ=38)                                                 cAAGCATTGCCACGGTCAGATCGAGTGATCTGAACCTTACGTTGTACAAGCCTGGGGTCGCTTTGCGGCCCTTTCGCAACACAAGGCCGCTCCCACAGGTACCGCGCTCGTCCATGAACGGGCGCGATCCTTGTGGGGGCGGCCTtgtg                                                                                                   >  1:123117/1‑149 (MQ=255)                                                 cAAGCATTGCCACGGTCAGATCGAGTGATCTGAACCTTACGTTGTACAAGCCTGGGGTCGCTTTGCGGCCCTTTCGCAACACAAGGCCGCTCCCACAGGTACCGCGCTCGTCCATGAACGGGCGCGATCCTTGTGGGGGCGGCCTtgtg                                                                                                   >  3:207025/1‑149 (MQ=255)                                                               gTCAGATCGAGTGATCTGAACCTTACGTTGTACAAGCCTGGCGTCGCTTTGCGGCCCTTTCGCAACACAAGGCCGCTCCCACAGGTACCGCGCTCGTCCATGAACGGGCGCGATCCTTGTGGGGGCGGCCTTGTGTCGCGATGGGGTGc                                                                                     <  5:231275/149‑1 (MQ=255)                                                                            aTCTGAACCTTACGTTGTACAAGCCTGGGGTCGCTTTGCGGCCCTTTCGCAACACAAGGCCGCTCCCACAGCTACCGCGCTCGTCCATGAACGGGCGCGATCCTTGTGGGGGCGGCCTTGTGTCGCGATGGGGTGCGAAGCGGCCCCtt                                                                        >  8:92811/1‑149 (MQ=255)                                                                                 aaCCTTACGTTGTACAAGCCTGGGGTCGCTTTGCGGCCCTTTCGCAACACAAGGCCGCTCCCACAGGTACCGCGCTCGTCCATGAACGGGCGCGATCCTTGTGGGGGCGGCCTTGTGTCGCGATGGGGTGCGAAGCGGCCCCTTTTTc                                                                    <  4:29492/148‑1 (MQ=255)                                                                                      tACGTTGTACAAGCCTGGGGTCGCTTTGCGGCCCTTTCGCAACACAAGGCCGCTCCCACAGGTACCGCGCTCGTCCATGAACGGGCGCGATCCTTGTgggg                                                                                                              >  1:92939/1‑101 (MQ=255)                                                                                      tACGTTGTACAAGCCTGGGGTCGCTTTGCGGCCCTTTCGCAACACAAGGCCGCTCCCACAGGTACCGCGCTCGTCCATGAACGGGCGCGATCCTTGTgggg                                                                                                              <  2:92939/101‑1 (MQ=255)                                                                                             tACAAGCCTGGGGTCGCTTTGCGGCCCTTTCGCAACACAAGGCCGCTCCCACAGGTACCGCGCTCGTCCATGAACGGGCGCGATCCTTGTGGGGGCGACCTAGTGTCGCGATGGGGTGCGAAGCGGCCCCTTTTTCGACATCATCGttt                                                       >  8:170087/1‑149 (MQ=255)                                                                                                aaGCCTGGGGTCGCTTTGCGGCCCTTTCGCAACACAAGGCCGCTCCCACAGGTACCGCGCTCGTCCATGAACGGGCGCGATCCTTGTGGGGGCGGCCTTGTGTCGCGATGGGGTGCGAAGCGGCCCCTTTTTCGACATCATCGttttt                                                     >  2:144084/1‑148 (MQ=255)                                                                                                   ccTGGGGTCGCTTTGCGGCCCTTTCGCAACACAAGGCCGCTCCCACAGGTACCGCGCTCGTCCATGAACGGGCGCGATCCTTGTGGGGGCGGCCTTGTGTCGCGATGGGGTGCGAAGCGGCCCCTTTTTCGAc                                                                 <  4:186642/133‑1 (MQ=255)                                                                                                    cTGGGGTCGGTTTGCGGCCCTTTCGCAACACAAGGCCGCTCCCACAGGTACCGCGCTCGTCCATGAACGGGCGCGATCCTTGTGGGGGCGGCCTTGTGTCGCGATGGGGTGCGAAGCGGCCCCTTTTTCGACATCATCGTTTTTTCTAg                                                <  5:169752/149‑1 (MQ=255)                                                                                                      ggggTCGCTTTGCGGCCCTTTCGCAACACAAGGCCGCTCCCACAGGTACCGCGCTCGTCCATGAACGGGCGCGATCCTTGTGGGGGCGGCCTTGTGTCGCGATGGGGTg                                                                                      <  1:14447/109‑1 (MQ=255)                                                                                                      ggggTCGCTTTGCGGCCCTTTCGCAACACAAGGCCGCTCCCACAGGTACCGCGCTCGTCCATGAACGGGCGCGATCCTTGTGGGGGCGGCCTTGTGTCGCGATGGGGTg                                                                                      >  2:14447/1‑109 (MQ=255)                                                                                                       gggTCGCTTTGCGGCCCTTTCGCAACACAAGGCCGCTCCCACAGGTACCGCGCTCGTCCATGAACGGGCGCGATCCTTGTGGGGGCGGCCTTGTGTCGCGATGGGGTGCGAAGCGGCCCCTTTTTCGACATCATCGTTTTTTCTAGGa                                              <  4:207025/148‑1 (MQ=255)                                                                                                          tCGCTTTGCGGCCCTTTCGCAACACAAGGCCGCTCCCACAGGTACCGCGCTCGTCCATGAACGGGCGCGATCCTTGTGGGGGCGGCCTTGTGTCGCGATGGGGTGCGAAGCGGCCCCTTTTTCGACATCATCGTTTTTTCTAGGAgcgc                                          >  2:91694/1‑149 (MQ=255)                                                                                                            gCTTTGCGGCCCTTTCGCAACACAAGGCCGCTCCCACAGGTACCGCGCTCGTCCATGAACGGGCGCGATCCTTGTGGGGGCGGCCTTGTGTCGCGATGGGGTGCGAAGCGGCCCCTTTTTCGACATCATCGTTTTTTCTAGGAGCGctc                                        <  3:191960/149‑1 (MQ=255)                                                                                                              tttGCGGCCCTTTCGCAACACAAGGCCGCTCCCACAGGTACCGCGCTCGTCCATGAACGGGCGCGATCCTTGTGGGGGCGGCCTTGTGTCGCGATGGGGTGCGAAGCGGCCCCTTTTTCGACATCATCGTTTTTTCTAGGAGCGctct                                       >  4:100028/1‑148 (MQ=255)                                                                                                                  cGGCCCTTTCGCAACACAAGGCCGCTCCCACAGGTACCGCGCTCGTCCATGAACGGGCGCGATCCTTGTGGGGGCGGCCTTGTGTCGCGATGGGGTGCGAAGCGGCCCGTTTTTCGACATCATCGTTTTTTATAGGAGCGAACTAATgg                                  >  6:228239/1‑149 (MQ=255)                                                                                                                  cGGCCCTTTCGCAACACAAGGCCGCTCCCACAGGTACCGCGCTCGTCCATGAACGGGCGCGATCCTTGTGGGGGCGGCCTTGTGTCGCGATGGGGTGCGAAGCGGCCCCTTTTTCGACATCATCGTTTTTTCTAGGAGCGCTCTAATg                                   <  1:91694/148‑1 (MQ=255)                                                                                                                   ggCCCTTTCGCAACACAAGGCCGCTCCCACAGGTACCGCGCTCGTCCATGAACGGGCGCGATCCTTGTGGGGGCGGCCTTGTGTCGCGATGGGGTGCGAAGCGGCCCCTTTTTCGACATCATCGTTTTTTCTAGGAGCGCTCTAATGGc                                 <  3:85408/149‑1 (MQ=255)                                                                                                                   ggCCCTTTCGCAACACAAGGCCGCTCCCACAGGTACCGCGCTCGTCCATGAACGGGCGCGATCCTTGTGGGGGCGGCCTTGTGTCGCGATGGGGTGCGAAGCGGCCCCTTTTTCGACATCATCGTTTTTTCTAGGAGCGCTCTAATGGc                                 >  2:34254/1‑149 (MQ=255)                                                                                                                        tttCGCAACACAAGGCCGCTCCCACAGGTACCGCGCTCGTCCATGAACGGGCGCGATCCTTGTGGGGGCGGCCTTGTGTCGCGATGGGGTGCGAAGCGGCCCCTTTTTCGACATCATCGTTTTTTCTAGGAGCGCTCTAATGGCTGttg                            >  2:208750/1‑149 (MQ=255)                                                                                                                        tttCGCAACACAAGGCCGCTCCCACAGGTACCGCGCTCGTCCATGAACGGGCGCGATCCTTGTGGGGGCGGCCTTGTGTCGCGATGGGGTGCGAAGCGGCCCCTTTTTCGACATCATCGTTTTTTCTAGGAGCGCTCTAATGGCTGttg                            >  8:28462/1‑149 (MQ=255)                                                                                                                           cGCAACACAAGGCCGCTCCCACAGGTACCGCGCTCGTCCATGAACGGGCGCGATCCTTGTGGCGCCGGCCTTGTGTCGCGATGGCGTGCCAAGCGGCCCCTTTTTCGACATCATCGTTTTTTCTAGGAGCGCTCTAATGGCTGttggtc                         >  5:65984/1‑149 (MQ=255)                                                                                                                                   aaGGCCGCTCCCACAGGTACCGCGCTCGTCCATGAACGGGCGCGATCCTTGTGGGGGCGGCCTTGTGTCGCGATGGGGTGCGAAGCGGCCCCTTTTTCGACATCATCGTTTTTTCTAGGAGCGCTCTAATGGCTGTTGGTCTTGGTCCt                 >  8:83168/1‑149 (MQ=255)                                                                                                                                     ggCCGCTCCCACAGGTACCGCGCTCGTCCATGAACGGGCGCGATCCTTGTGGGGGCGGCCTTGTGTCGCGATGGGGTGCGAAGCGGCCCCTTTTTCGACATCATCGTTTTTTCTAGGAGCGCTCTAATGGCTGTTGGTCTTGGTCCt                 <  6:248026/147‑1 (MQ=255)                                                                                                                                     ggCCGCCCCCACAAGGATCGCGCCCGTTCATGGACGAGCGCGGTACCTGTGGGAGCGGCCTTGTGTCGCGATGGGGTGCGAAGCGGCCCCTTTTTCGACATCATCGTTTTTTCTAGGAGCGCTCTAATGGCTGTTGGTCTTGGTCCttt               >  4:52844/1‑149 (MQ=18)                                                                                                                                        cGCTCCCACAGGTACCGCGCTCGTCCATGAACGGGCGCGATCCTTGTGGGGGCGGCCTTGTGTCGCGATGGGGTGCGAAGCGGCCCCTTTTTCGACATCATCGTTTTTTCTAGGAGCGCTCTAATGGCTGTTGGTCTTGGTCCTTTGcc            <  1:34254/149‑1 (MQ=255)                                                                                                                                        cGCCCCCACAAGGATCGCGCCCGTTCATGGACGAGCGCGGTACCTGTGGGAGCGGCCTTGTGTTGCGATGGGGTGCGAAGCGGCCCCTTTTTCGACATCATCGTTTTTTCTAGGAGCGCTCTAATGGCTGTTGGTCTTGGTCCTTTGcc            >  4:67407/1‑149 (MQ=18)                                                                                                                                         gCTCCCACAGGTACCGCGCTCGTCCATGAACGGGCGCGATCCTTGTGGGGGCGGCCTTGTGTCGCGATGGGGTGCGAAGCGGCCCCTTTTTCGACATCATCGTTTTTTCTAGGAGCGCTCTAATGGCTGTTGGTCTTGGTCCTTTGcc            >  8:114197/1‑148 (MQ=255)                                                                                                                                            cccACAGGTACCGCGCTCGTCCATGAACGGGCGCGATCCTTGTGGGGGCGGCCTTGTGTCGCGATGGGGTGCGAAGCGGCCCCTTTTTCGACATCATCGTTTTTTCTAGGAGCGCTCTAATGGCTGTTGGTCTTGGTCCTTTGCCCAcc        >  6:184744/1‑149 (MQ=255)                                                                                                                                                  ggTACCGCGCTCGTCCATGAACGGGCGCGATCCTTGTGGGGGCGGCCTTGTGTCGCGATGGGGTGCGAAGCGGCCCCTTTTTCGACATCATCGTTTTTTCTAGGAGCGCTCTAATGGCTGTTGGTCTTGGTCCTTTGCCCACCCTGCAc  >  3:44606/1‑149 (MQ=255)                                                                                                                                                  |                                                                                                                                                     GAAGCTGGGCCGCAACGAGCCGTGCTGGTGTGGTTCGGGCAAGAAGTTCAAGCATTGCCACGGTCAGATCGAGTGATCTGAACCTTACGTTGTACAAGCCTGGGGTCGCTTTGCGGCCCTTTCGCAACACAAGGCCGCTCCCACAGGTACCGCGCTCGTCCATGAACGGGCGCGATCCTTGTGGGGGCGGCCTTGTGTCGCGATGGGGTGCGAAGCGGCCCCTTTTTCGACATCATCGTTTTTTCTAGGAGCGCTCTAATGGCTGTTGGTCTTGGTCCTTTGCCCACCCTGCAC  >  NC_002947/1534734‑1535027

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑