Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,534,902	G→A	13.7%	intergenic (+93/‑90)	secA → / → argJ	protein translocase subunit SecA/glutamate N‑acetyltransferase/amino acid acetyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,534,902	0	G	A	13.7%	146.3 / 12.8	58	intergenic (+93/‑90)	secA/argJ	protein translocase subunit SecA/glutamate N‑acetyltransferase/amino acid acetyltransferase
Reads supporting (aligned to +/- strand):  ref base G (26/24);  new base A (3/5);  total (29/29)
Fisher's exact test for biased strand distribution p-value = 7.06e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.55e-01

TGCTGGTGTGGTTCGGGCAAGAAGTTCAAGCATTGCCACGGTCAGATCGAGTGATCTGAACCTTACGTTGTACAAGCCTGGGGTCGCTTTGCGGCCCTTTCGCAACACAAGGCCGCTCCCACAGGTACCGCGCTCGTCCATGAACGGGCGCGATCCTTGTGGGGGCGGCCTTGTGTCGCGATGGGGTG‑CGAAGCGGCCCCTTTTTCGACATCATCGTTTTTTCTAGGAGCGCTCTAATGGCTGTTGGTCTTGGTCCTTTGCCCACCCTGCACCCGGTTCCGGGTTTTGAACTC  >  NC_002947/1534756‑1535048                                                                                                                                                   |                                                                                                                                                    tGCTGGTGTGGTTCTGGCAAGAAGTTCAAGCATTGCCACGGTCAGATCGAGTGATCTGAACCTTACGTTGTACAAGCCTGGGGGCGCTTCGC‑ACCCCATCGCGACACAAGGCCGCCCCCACAAGGATCGCGCCCGTTCATGGACGAgcg                                                                                                                                                  <  5:71276/149‑1 (MQ=17) tGCTGGTGTGGTTCGGGCAAGAAGTTCAAGCATTGCCACGGTCAGATCGAGTGATCTGAACCTTACGTTGTACAAGCCTGGGGTCGCTTCGC‑ACCCCATCGCGACACAAGGCCGCCCCCACAAGGATCGCGCCCGTTCATGGACGAgcg                                                                                                                                                  <  5:74994/149‑1 (MQ=18)       tgtgGTTCGGGCAAGAAGTTCAAGCATTGCCACGGTCAGATCGAGTGATCTGAACCTTACGTTGTACAAGCCTGGGGTCGCTTTGCGGCCCTTTCGCAACACAAGGCCGCTCCCACAGGTACCGCGCTCGTCCATGAACGGGCGCGATc                                                                                                                                             >  2:208306/1‑149 (MQ=255)           gTTCGGGCAAGAAGTTCAAGCATTGCCACGGTCAGATCGAGTGATCTGAACCTTACGTTGTACAAGCCTGGGGTCGCTTTGCGGCCCTTTCGCAACACAAGGCCGCTCCCACAGGTACCGCGCTCGTCCATGAACGGGCGCGATcc                                                                                                                                            <  4:58608/146‑1 (MQ=255)           gTTCGGGCAAGAAGTTCAAGCATTGCCACGGTCAGATCGAGTGATCTGAACCTTACGTTGTACAAGCCTGGGGTCGCTTTGCGGCCCTTTCGCAACACAAGGCCGCTCCCACAGGTACCGCGCTCGTCCATGAACGGGCGCGATcc                                                                                                                                            >  3:58608/1‑146 (MQ=255)           gTTCGGGCAAGAAGTTCAAGCATTGCCACGGTCAGATCGAGTGATCTGAACCTTACGTTGTACAAGCCTGGGGTCGCTTTGCGGCCCTTTCGCAACACAAGGCCGCTCCCACAGGTACCGCGCTCGTCCATGAACGGGCGCGATCCTtg                                                                                                                                         >  4:191960/1‑149 (MQ=255)           gTTCGGGCAAGAAGTTCAAGCATTGCCACGGTCAGATCGAGTGATCTGAACCTTACGTTGTACAAGCCTGGGGTCGCTTTGCGGCCCTTTCGCAACACAAGGCCGCTCCCACAGGTACCGCGCTCGTCCATGAACGGGCGCGATCCTtg                                                                                                                                         >  5:248026/1‑149 (MQ=255)           gTTCGGGCAAGAAGTTCAAGCATTGCCACGGTCAGATCGAGTGATCTGAACCTTACGTTGTACAAGCCTGGGGTCGCTTTGCGGCCCTTTCGCAACACAAGGCCGCCCCCACAAGGATCGCGCCCGTTCATGGACGAGcgcggtacctg                                                                                                                                         <  2:259415/149‑8 (MQ=25)              cGGGCAAGAAGTTCAAGCATTGCCACGGTCAGATCGAGTGATCTGAACCTTACGTTGTACAAGCCTGGGGTCGCTTTGCGGCCCTTTCGCAACACAAGGCCGCTCCCACAGGTACCGCGCTCGTCCATGAACGGGCGCGATCCTtgtg                                                                                                                                       <  6:232851/148‑1 (MQ=255)               gggCAAGAAGTTCAAGCATTGCCACGGTCAGATCGAGTGATCTGAACCTTACGTTGTACAAGCCTGAGGTCGCTTTGCGGCCCTTTCGCAACACAAGGCCGCTCCCACAGGTACCGCGCTCGTCCATGAACGGGCGCGATCCTTGTgg                                                                                                                                      <  5:108161/148‑1 (MQ=255)                  cAAGAAGTTCAAGCATTGCCACGGTCAGATCGAGTGATCTGAACCTTACGTTGTACAAGCCTGGGGTCGCTTTGCGGCCCTTTCGCAACACAAGGCCGCTCCCACAGGTACCGCGCTCGTCCATGAACGGGCGCGATCCTTGTGGGggc                                                                                                                                  >  5:53106/1‑149 (MQ=255)                       agTTCAAGCATTGCCACGGTCAGATCGAGTGATCTGAACCTTACGTTGTACAAGCCTGGGGTCGCTTTGCGGCCCTTTCGCAACACAAGGCCGCTCCCACAGGTACCGCGCTCGTCCATGAACGGGCGCGATCCTTGTGGGGGCGGcc                                                                                                                              <  6:53106/148‑1 (MQ=255)                        gTTCAAGCATTGCCACGGTCAGATCGAGTGATCTGAACCTTACGTTGTACAAGCCTGGGGTCGCTTTGCGGCCCTTTCGCAACACAAGGCCGCTCCCACAGGTACCGCGCTCGTCCATGAACGGGCGCGATCCTTGTGGGGGCGGCCtt                                                                                                                            <  7:221345/149‑1 (MQ=255)                         ttCAAGCATTGCCACGGTCAGATCGAGTGATCTGAACCTTACGTTGTACAAGCCTGGGGTCGCTTTGCGGCCCTTTCGCAACACAAGGCCGCTCCCACAGGTACCGCGCTCGTCCATGAACGGGCGCGATCCTTGTGGGGGCGGCCTtg                                                                                                                           >  5:77243/1‑149 (MQ=255)                         ttCAAGCATTGCCACGGTCAGATCGAGTGATCTGAACCTTACGTTGTACAAGCCTGGGGTCGCTTTGCGGCCCTTTCGCAACACAAGGCCGCTCCCACAGGTACCGCGCCCGTTCATGGACGAGcgcgg                                                                                                                                               >  2:208159/1‑128 (MQ=38)                         ttCAAGCATTGCCACGGTCAGATCGAGTGATCTGAACCTTACGTTGTACAAGCCTGGGGTCGCTTTGCGGCCCTTTCGCAACACAAGGCCGCTCCCACAGGTACCGCGCCCGTTCATGGACGAGcgcgg                                                                                                                                               <  1:208159/129‑2 (MQ=38)                           cAAGCATTGCCACGGTCAGATCGAGTGATCTGAACCTTACGTTGTACAAGCCTGGGGTCGCTTTGCGGCCCTTTCGCAACACAAGGCCGCTCCCACAGGTACCGCGCTCGTCCATGAACGGGCGCGATCCTTGTGGGGGCGGCCTtgtg                                                                                                                         >  3:207025/1‑149 (MQ=255)                           cAAGCATTGCCACGGTCAGATCGAGTGATCTGAACCTTACGTTGTACAAGCCTGGGGTCGCTTTGCGGCCCTTTCGCAACACAAGGCCGCTCCCACAGGTACCGCGCTCGTCCATGAACGGGCGCGATCCTTGTGGGGGCGGCCTtgtg                                                                                                                         >  1:123117/1‑149 (MQ=255)                                         gTCAGATCGAGTGATCTGAACCTTACGTTGTACAAGCCTGGCGTCGCTTTGCGGCCCTTTCGCAACACAAGGCCGCTCCCACAGGTACCGCGCTCGTCCATGAACGGGCGCGATCCTTGTGGGGGCGGCCTTGTGTCGCGATGGGGTG‑c                                                                                                          <  5:231275/149‑1 (MQ=255)                                                      aTCTGAACCTTACGTTGTACAAGCCTGGGGTCGCTTTGCGGCCCTTTCGCAACACAAGGCCGCTCCCACAGCTACCGCGCTCGTCCATGAACGGGCGCGATCCTTGTGGGGGCGGCCTTGTGTCGCGATGGGGTG‑CGAAGCGGCCCCtt                                                                                             >  8:92811/1‑149 (MQ=255)                                                           aaCCTTACGTTGTACAAGCCTGGGGTCGCTTTGCGGCCCTTTCGCAACACAAGGCCGCTCCCACAGGTACCGCGCTCGTCCATGAACGGGCGCGATCCTTGTGGGGGCGGCCTTGTGTCGCGATGGGGTG‑CGAAGCGGCCCCTTTTTc                                                                                         <  4:29492/148‑1 (MQ=255)                                                                tACGTTGTACAAGCCTGGGGTCGCTTTGCGGCCCTTTCGCAACACAAGGCCGCTCCCACAGGTACCGCGCTCGTCCATGAACGGGCGCGATCCTTGTgggg                                                                                                                                    <  2:92939/101‑1 (MQ=255)                                                                tACGTTGTACAAGCCTGGGGTCGCTTTGCGGCCCTTTCGCAACACAAGGCCGCTCCCACAGGTACCGCGCTCGTCCATGAACGGGCGCGATCCTTGTgggg                                                                                                                                    >  1:92939/1‑101 (MQ=255)                                                                       tACAAGCCTGGGGTCGCTTTGCGGCCCTTTCGCAACACAAGGCCGCTCCCACAGGTACCGCGCTCGTCCATGAACGGGCGCGATCCTTGTGGGGGCGACCTAGTGTCGCGATGGGGTG‑CGAAGCGGCCCCTTTTTCGACATCATCGttt                                                                            >  8:170087/1‑149 (MQ=255)                                                                          aaGCCTGGGGTCGCTTTGCGGCCCTTTCGCAACACAAGGCCGCTCCCACAGGTACCGCGCTCGTCCATGAACGGGCGCGATCCTTGTGGGGGCGGCCTTGTGTCGCGATGGGGTG‑CGAAGCGGCCCCTTTTTCGACATCATCGttttt                                                                          >  2:144084/1‑148 (MQ=255)                                                                             ccTGGGGTCGCTTTGCGGCCCTTTCGCAACACAAGGCCGCTCCCACAGGTACCGCGCTCGTCCATGAACGGGCGCGATCCTTGTGGGGGCGGCCTTGTGTCGCGATGGGGTG‑CGAAGCGGCCCCTTTTTCGAc                                                                                      <  4:186642/133‑1 (MQ=255)                                                                              cTGGGGTCGGTTTGCGGCCCTTTCGCAACACAAGGCCGCTCCCACAGGTACCGCGCTCGTCCATGAACGGGCGCGATCCTTGTGGGGGCGGCCTTGTGTCGCGATGGGGTG‑CGAAGCGGCCCCTTTTTCGACATCATCGTTTTTTCTAg                                                                     <  5:169752/149‑1 (MQ=255)                                                                                ggggTCGCTTTGCGGCCCTTTCGCAACACAAGGCCGCTCCCACAGGTACCGCGCTCGTCCATGAACGGGCGCGATCCTTGTGGGGGCGGCCTTGTGTCGCGATGGGGTg                                                                                                            <  1:14447/109‑1 (MQ=255)                                                                                ggggTCGCTTTGCGGCCCTTTCGCAACACAAGGCCGCTCCCACAGGTACCGCGCTCGTCCATGAACGGGCGCGATCCTTGTGGGGGCGGCCTTGTGTCGCGATGGGGTg                                                                                                            >  2:14447/1‑109 (MQ=255)                                                                                 gggTCGCTTTGCGGCCCTTTCGCAACACAAGGCCGCTCCCACAGGTACCGCGCTCGTCCATGAACGGGCGCGATCCTTGTGGGGGCGGCCTTGTGTCGCGATGGGGTG‑CGAAGCGGCCCCTTTTTCGACATCATCGTTTTTTCTAGGa                                                                   <  4:207025/148‑1 (MQ=255)                                                                                    tCGCTTTGCGGCCCTTTCGCAACACAAGGCCGCTCCCACAGGTACCGCGCTCGTCCATGAACGGGCGCGATCCTTGTGGGGGCGGCCTTGTGTCGCGATGGGGTG‑CGAAGCGGCCCCTTTTTCGACATCATCGTTTTTTCTAGGAgcgc                                                               >  2:91694/1‑149 (MQ=255)                                                                                      gCTTTGCGGCCCTTTCGCAACACAAGGCCGCTCCCACAGGTACCGCGCTCGTCCATGAACGGGCGCGATCCTTGTGGGGGCGGCCTTGTGTCGCGATGGGGTG‑CGAAGCGGCCCCTTTTTCGACATCATCGTTTTTTCTAGGAGCGctc                                                             <  3:191960/149‑1 (MQ=255)                                                                                        tttGCGGCCCTTTCGCAACACAAGGCCGCTCCCACAGGTACCGCGCTCGTCCATGAACGGGCGCGATCCTTGTGGGGGCGGCCTTGTGTCGCGATGGGGTG‑CGAAGCGGCCCCTTTTTCGACATCATCGTTTTTTCTAGGAGCGctct                                                            >  4:100028/1‑148 (MQ=255)                                                                                            cGGCCCTTTCGCAACACAAGGCCGCTCCCACAGGTACCGCGCTCGTCCATGAACGGGCGCGATCCTTGTGGGGGCGGCCTTGTGTCGCGATGGGGTG‑CGAAGCGGCCCGTTTTTCGACATCATCGTTTTTTATAGGAGCGAACTAATgg                                                       >  6:228239/1‑149 (MQ=255)                                                                                            cGGCCCTTTCGCAACACAAGGCCGCTCCCACAGGTACCGCGCTCGTCCATGAACGGGCGCGATCCTTGTGGGGGCGGCCTTGTGTCGCGATGGGGTG‑CGAAGCGGCCCCTTTTTCGACATCATCGTTTTTTCTAGGAGCGCTCTAATg                                                        <  1:91694/148‑1 (MQ=255)                                                                                             ggCCCTTTCGCAACACAAGGCCGCTCCCACAGGTACCGCGCTCGTCCATGAACGGGCGCGATCCTTGTGGGGGCGGCCTTGTGTCGCGATGGGGTG‑CGAAGCGGCCCCTTTTTCGACATCATCGTTTTTTCTAGGAGCGCTCTAATGGc                                                      <  3:85408/149‑1 (MQ=255)                                                                                             ggCCCTTTCGCAACACAAGGCCGCTCCCACAGGTACCGCGCTCGTCCATGAACGGGCGCGATCCTTGTGGGGGCGGCCTTGTGTCGCGATGGGGTG‑CGAAGCGGCCCCTTTTTCGACATCATCGTTTTTTCTAGGAGCGCTCTAATGGc                                                      >  2:34254/1‑149 (MQ=255)                                                                                                  tttCGCAACACAAGGCCGCTCCCACAGGTACCGCGCTCGTCCATGAACGGGCGCGATCCTTGTGGGGGCGGCCTTGTGTCGCGATGGGGTG‑CGAAGCGGCCCCTTTTTCGACATCATCGTTTTTTCTAGGAGCGCTCTAATGGCTGttg                                                 >  2:208750/1‑149 (MQ=255)                                                                                                  tttCGCAACACAAGGCCGCTCCCACAGGTACCGCGCTCGTCCATGAACGGGCGCGATCCTTGTGGGGGCGGCCTTGTGTCGCGATGGGGTG‑CGAAGCGGCCCCTTTTTCGACATCATCGTTTTTTCTAGGAGCGCTCTAATGGCTGttg                                                 >  8:28462/1‑149 (MQ=255)                                                                                                     cGCAACACAAGGCCGCTCCCACAGGTACCGCGCTCGTCCATGAACGGGCGCGATCCTTGTGGCGCCGGCCTTGTGTCGCGATGGCGTG‑CCAAGCGGCCCCTTTTTCGACATCATCGTTTTTTCTAGGAGCGCTCTAATGGCTGttggtc                                              >  5:65984/1‑149 (MQ=255)                                                                                                             aaGGCCGCTCCCACAGGTACCGCGCTCGTCCATGAACGGGCGCGATCCTTGTGGGGGCGGCCTTGTGTCGCGATGGGGTG‑CGAAGCGGCCCCTTTTTCGACATCATCGTTTTTTCTAGGAGCGCTCTAATGGCTGTTGGTCTTGGTCCt                                      >  8:83168/1‑149 (MQ=255)                                                                                                               ggCCGCTCCCACAGGTACCGCGCTCGTCCATGAACGGGCGCGATCCTTGTGGGGGCGGCCTTGTGTCGCGATGGGGTG‑CGAAGCGGCCCCTTTTTCGACATCATCGTTTTTTCTAGGAGCGCTCTAATGGCTGTTGGTCTTGGTCCt                                      <  6:248026/147‑1 (MQ=255)                                                                                                               ggCCGCCCCCACAAGGATCGCGCCCGTTCATGGACGAGCGCGGTACCTGTGGGAGCGGCCTTGTGTCGCGATGGGGTG‑CGAAGCGGCCCCTTTTTCGACATCATCGTTTTTTCTAGGAGCGCTCTAATGGCTGTTGGTCTTGGTCCttt                                    >  4:52844/1‑149 (MQ=18)                                                                                                                  cGCTCCCACAGGTACCGCGCTCGTCCATGAACGGGCGCGATCCTTGTGGGGGCGGCCTTGTGTCGCGATGGGGTG‑CGAAGCGGCCCCTTTTTCGACATCATCGTTTTTTCTAGGAGCGCTCTAATGGCTGTTGGTCTTGGTCCTTTGcc                                 <  1:34254/149‑1 (MQ=255)                                                                                                                  cGCCCCCACAAGGATCGCGCCCGTTCATGGACGAGCGCGGTACCTGTGGGAGCGGCCTTGTGTTGCGATGGGGTG‑CGAAGCGGCCCCTTTTTCGACATCATCGTTTTTTCTAGGAGCGCTCTAATGGCTGTTGGTCTTGGTCCTTTGcc                                 >  4:67407/1‑149 (MQ=18)                                                                                                                   gCTCCCACAGGTACCGCGCTCGTCCATGAACGGGCGCGATCCTTGTGGGGGCGGCCTTGTGTCGCGATGGGGTG‑CGAAGCGGCCCCTTTTTCGACATCATCGTTTTTTCTAGGAGCGCTCTAATGGCTGTTGGTCTTGGTCCTTTGcc                                 >  8:114197/1‑148 (MQ=255)                                                                                                                      cccACAGGTACCGCGCTCGTCCATGAACGGGCGCGATCCTTGTGGGGGCGGCCTTGTGTCGCGATGGGGTG‑CGAAGCGGCCCCTTTTTCGACATCATCGTTTTTTCTAGGAGCGCTCTAATGGCTGTTGGTCTTGGTCCTTTGCCCAcc                             >  6:184744/1‑149 (MQ=255)                                                                                                                            ggTACCGCGCTCGTCCATGAACGGGCGCGATCCTTGTGGGGGCGGCCTTGTGTCGCGATGGGGTG‑CGAAGCGGCCCCTTTTTCGACATCATCGTTTTTTCTAGGAGCGCTCTAATGGCTGTTGGTCTTGGTCCTTTGCCCACCCTGCAc                       >  3:44606/1‑149 (MQ=255)                                                                                                                             gTACCGCGCTCGTCCATGAACGGGTGCGATGCTTGTGGGGGCGGCCTTGTGTCGCGATGGGGTG‑CGAAGCGGCCCCTTTTTCGACATCATCGTTTTTTCTAGGAGCGCTCTAATGGCTGTTGGTCTTGGTCCTTTGCCCACCCTGCAcc                      <  6:77243/149‑1 (MQ=255)                                                                                                                              tACCGCGCTCGTGCATGATCGGGGGCGATCGTTGTGGGGGCGGCCTTGTGTCGCGATGGGGTG‑CGAAGCGGCCCCTTTTTCGACATCATCGTTTTTTCTAGGAGCGCTCTAATGGCTGTTGGTCTTGGTCCTTTGCCCACCCTGCAccc                     <  6:65984/149‑1 (MQ=255)                                                                                                                                 cgcgCTCGTCCATGAACGGGCGCGATCCTTGTGGGGGCGGCCTTGTGTCGCGATGGGGTG‑CGAAGCGGCCCCTTTTTCGACATCATCGTTTTTTCTAGGAGCGCTCTAATGGCTGTTGGTCTTGGTCATTTGCGCACCCTGCACCCGGt                  >  6:182503/1‑149 (MQ=255)                                                                                                                                        gTCCATGAACGGGCGCGATCCTTGTGGGGGCGGCCTTGTGTCGCGATGGGGTG‑CGAAGCGGCCCCTTTTTCGACATCATCGTTTTTTCTAGGAGCGCTCTAATGGCTGTTGGTCTTGGTCCTTTGCCCACCCTGCACCCGGTTCCgg             <  7:83168/147‑1 (MQ=255)                                                                                                                                        gTCCATGAACGGGCGCGATCCTTGTGGGGGCGGCCTTGTGTCGCGATGGGGTG‑CGAAGCGGCCCCTTTTTCGACATCATCGTTTTTTCTAGGAGCGCTCTAATGGCTGTTGGTCTTGGTCCTTTGCCCACCCTGCACCCGGTTCCgg             <  1:208750/147‑1 (MQ=255)                                                                                                                                        gTCCATGAACGGGCGCGATCCTTGTGGGGGCGGCCTTGTGTCGCGATGGGGTG‑CGAAGCGGCCCCTTTTTCGACATCATCGTTTTTTCTAGGAGCGCTCTAATGGCTGTGGGTCTTGGTCCTTTGCCCACCCTGCACCCGGTTCCGGGt           >  8:99411/1‑149 (MQ=255)                                                                                                                                          ccATGAACGGGCGCGATCCTTGTGGGGGCGGCCTTGTGTCGCGATGGGGTG‑CGAAGCGGCCCCTTTTTCGACATCATCGTTTTTTCTAGGAGCGCTCTAATGGCTGTTGGTCTTGGTCCTTTGCCCACCCTGCACCCGGTTCCGGGttt         <  7:92811/149‑1 (MQ=255)                                                                                                                                           cATGGACGAGCGCGGTACCTGTGGGAGCGGCCTTGTGTCGCGATGGGGTG‑CGAAGCGGCCCCTTTTTCGACATCATCGTTTTTTCTAGGAGCGCTCTAATGGCTGTTGGTCTTGGTCCTTTGCCCACCCTGCACCCGGTTCCGGGttt         <  3:52844/148‑1 (MQ=38)                                                                                                                                               aaCGGGCGCGATCCTTGTGGGGGCGGCCTTGTGTCGCGATGGGGTG‑CGAAGCGGCCCCTTTTTCGACATCATCGTTTTTTCTAGGAGCGCTCTAATGGCTGTTGGTCTTGGTCCTTTGCCCACCCTGCACCCGGTTCCGGGTTTTGAAc    <  1:208306/149‑1 (MQ=255)                                                                                                                                                 cGGGCGCGATCCTTGTGGGGGCGGCCTTGTTTCGCGTTGGGGTG‑CGAAGCGGCCCCTTTTTCGACATCATCGTTTTTTCTAGGAGCGCTCTAATGGCTGTTGGTCTTGGTCCTTTGCCCACCCTGCACCCGGTTCCGGGTTTTGAACTc  <  5:228239/149‑1 (MQ=255)                                                                                                                                                  gggCGCGATCCTTGTGGGGGCGGCCTTGTGTCGCGATGGGGTGTCGAAGCGGCCCCTTTTTCGACATCATCGTTTTTTCTAGGAGCGGTCTAATGGCTGTTGGTCTTGGTCCTTTGCCCACCCTGCACCCGGTTCCGGGTTTTGAACTc  >  1:188410/1‑149 (MQ=255)                                                                                                                                                   |                                                                                                                                                    TGCTGGTGTGGTTCGGGCAAGAAGTTCAAGCATTGCCACGGTCAGATCGAGTGATCTGAACCTTACGTTGTACAAGCCTGGGGTCGCTTTGCGGCCCTTTCGCAACACAAGGCCGCTCCCACAGGTACCGCGCTCGTCCATGAACGGGCGCGATCCTTGTGGGGGCGGCCTTGTGTCGCGATGGGGTG‑CGAAGCGGCCCCTTTTTCGACATCATCGTTTTTTCTAGGAGCGCTCTAATGGCTGTTGGTCTTGGTCCTTTGCCCACCCTGCACCCGGTTCCGGGTTTTGAACTC  >  NC_002947/1534756‑1535048

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑