Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,555,390 G→T 13.2% intergenic (+28/+46) sbcB → / ← PP_1366 exodeoxyribonuclease I/transcriptional regulator MvaT

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,555,3900GT13.2% 138.8 / 13.5 53intergenic (+28/+46)sbcB/PP_1366exodeoxyribonuclease I/transcriptional regulator MvaT
Reads supporting (aligned to +/- strand):  ref base G (21/25);  new base T (4/3);  total (25/28)
Fisher's exact test for biased strand distribution p-value = 6.94e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.72e-01

GAAAAGGCGCGCCAGCAAGCCTGGGAGGGCGCCGACGAGGCAGGCAGGCGCGTGTTGGAGGCCTGGCAACTGCATGCTCGACAATTGCAGGAACGCTATTCAATCGGCTGA‑TGGCAGATAAACAAAAACGCCGGCAATGTGCCGGCGTTTTTGTTGTGCGAAGCAAGAGGGCGTATGCGGATAATCAGTCCAGCAGGGTTGCCCAGCTTTCAACCACGTCGCCGCCCCAAGTGGCTTTCCACTCTTTCAGGGTCTTGTGGTTGCCGCCTTTGGTTTCGATC  >  NC_002947/1555252‑1555532
                                                                                                                                           |                                                                                                                                              
gAAAAGGCGCGCCAGCAAGCCTGGGAGGGCGCCGACGAGGCAGGCAGGCGCGTGTTGGAGGCCTGGCAACTGCATGCTCGACAATTGCAGGAACGCTATTCAATCGGCTGA‑TGGCAGATAAACAAAAACGCCGGCAATGTGCCGGCGtt                                                                                                                                      >  3:9815/1‑149 (MQ=255)
gAAAAGGCGCGCCAGCAAGCCTGGGAGGGCGCCGACGAGGCAGGCAGGCGCGTGTTGGAGGCCTGGCAACTGCATGCTCGACAATTGCAGGAACGCTATTCAATCGGCTGA‑TGGCAGATAAACAAAAACGCCGGCAATGTGCCGGCGt                                                                                                                                       <  6:241919/148‑1 (MQ=255)
 aaaaGGCGCGCCAGCAAGCCTGGGAGGGCGCCGACGAGGCAGGCAGGCGCGTGTTGGAGGCCTGGCAACTGCATGCTCGACAATTGCAGGAACGCTATTCAATCGGCTGA‑TGGCAGATAAACAAAAACGCCGGCAATGTGCCGGCGttt                                                                                                                                     <  7:123193/149‑1 (MQ=255)
      gcgcgcCAGCAAGCCTGGGAGGGCGCCGACGAGGCAGGCAGGCGCGTGTTGGAGGCCTGGCAACTGCATGCTCGACAATTGCAGGAACGCTATTCAATCGGCTGACTGGCAGATAAACAAAAACGCCGGCACATTGCCGGCGTTTttgt                                                                                                                                 <  1:187756/149‑1 (MQ=255)
        gcgcCAGCAAGCCTGGGAGGGCGCCGACGAGGCAGGCAGGCGCGTGTTGGAGGCCTGGCAACTGCATGCTCGACAATTGCAGGAACGCTATTCAATCGGCTGA‑TGGCAGATAAACAAAAACGCCGGCACATTGCCGGCGTTTttgttta                                                                                                                              >  3:76872/1‑147 (MQ=255)
        gcgcCAGCAAGCCTGGGAGGGCGCCGACGAGGCAGGCAGGCGCGTGTTGGAGGCCTGGCAACTGCATGCTCGACAATTGCAGGAACGCTATTCAATCGGCTGA‑TGGCAGATAAACAAAAACGCCGGCAATGTGCCGGCGTTTTtgttgt                                                                                                                              <  6:103000/149‑1 (MQ=255)
          gcCAGCAAGCCTGGGAGGGCGCCGACGAGGCAGGCAGGCGCGTGTTGGAGGCCTGGCAACTGCATGCTCGACAATTGCAGGAACGCTATTCAATCGGCTGA‑TGGCAGATAAACAAAAACGCCGGCAATGTGCCGGCGTTTTTGTTGTGc                                                                                                                            <  8:192216/149‑1 (MQ=255)
                aaGCCTGGGAGGGCGCCGACGAGGCAGGCAGGCGCGTGTTGGAGGCCTGGCAACTGCATGCTCGACAATTGCAGGAACGCTATTCAATCGGCTGA‑TGGCAGATAAACAAAAACGCCGGCAATGTGCCGGCGTTTTTGTTGTGCGAAGc                                                                                                                       >  5:181057/1‑148 (MQ=255)
                    cTGGGAGGGCGCCGACGAGGCAGGCAGGCGCGTGTTGGAGGCCTGGCAACTGCATGCTCGACAATTGCAGGAACGCTATTCAATCGGCTGA‑TGGCAGATAAACAAAAACGCCGGCAATGTGCCGGCGTTTTTGTTGTGCGAAGAAagag                                                                                                                  >  5:141914/1‑149 (MQ=255)
                                   cgaGGCAGGCAGGCGCGTGTTGGAGGCCTGGCAACTGCATGCTCGACAATTGCAGGAACGCTATTCAATCGGCTGA‑TGGCAGATAAACAAAAACGCCGGCAATGTGCCGGCGTTTTTGTTGTGCGAAGCAAGAGGGCGTATGCGGATaa                                                                                                   <  4:9815/149‑1 (MQ=255)
                                         aggcaggcGCGTGTTGGAGGCCTGGCAACTGCATGCTCGACAATTGCAGGAACGCTATTCAATCGGCTGA‑TGGCAGATAAACAAAAACGCCGGCAATGTGCCGGCGTTTTTGTTGTGCGAAGCAAGAGGGCGTATGCGGATAATCa                                                                                                <  6:235072/146‑1 (MQ=255)
                                         aggcaggcGCGTGTTGGAGGCCTGGCAACTGCATGCTCGACAATTGCAGGAACGCTATTCAATCGGCTGA‑TGGCAGATAAACAAAAACGCCGGCAATGTGCCGGCGTTTTTGTTGTGCGAAGCAAGAGGGCGTATGCGGATAATCa                                                                                                >  5:235072/1‑146 (MQ=255)
                                         aggcaggcGCGTGTTGGAGGCCTGGCAACTGCATGCTCGACAATTGCAGGAACGCTATTCAATCGGCTGA‑TGGCAGATAAACAAAAACGCCGGCAATGTGCCGGCGTTTTTGTTGTGCGAAGCAAGAGGGCGTATGCGGATAATCAGTc                                                                                             >  6:266707/1‑149 (MQ=255)
                                                cgcgTGTTGGAGGCCTGGCAACTGCATGCTCGACAATTGCAGGAACGCTATTCAATCGGCTGA‑TGGCAGATAAACAAAAACGCCGGCAATGTGCCGGCGTTTTTGTTGTGCGAAGCAAGAGGGCGTATGCGGATAATCAGTCCAGCAgg                                                                                      <  5:266707/149‑1 (MQ=255)
                                                 gcgTGTTGGAGGCCTGGCAACTGCATGCTCGACAATTGCAGGAACGCTATTCAATCGGCTGA‑TGGCAGATAAACAAAAACGCCGGCAATGTGCCGGCGTTTTTGTTGTGCGAAGCAAGAGGGCGTATGCGGATAATCAGTCCAGCAggg                                                                                     >  7:3138/1‑149 (MQ=255)
                                                      ttGGAGGCCTGGCAACTGCATGCTCGACAATTGCAGGAACGCTATTCAATCGGCTGA‑TGGCAGATAAACAAAAACGCCGGCAATGTGCCGGCGTTTTTGTTGTGCGAAGCAAGAGGGCGTATGCGGATAATCAGTCCAGCAGGGTTGc                                                                                 <  8:3138/148‑1 (MQ=255)
                                                              cTGGCAACTGCATGCTCGACAATTGCAGGAACGCTATTCAATCGGCTGA‑TGGCAGATAAACAAAAACGCCGGCACATTGCCGGCGttttt                                                                                                                                   >  1:116043/1‑90 (MQ=38)
                                                              cTGGCAACTGCATGCTCGACAATTGCAGGAACGCTATTCAATCGGCTGA‑TGGCAGATAAACAAAAACGCCGGCACATTGCCGGCGttttt                                                                                                                                   <  2:116043/90‑1 (MQ=38)
                                                              cTGGCAACTGCATGCTCGACAATTGCAGGAACGCTATTCAATCGGCTGA‑TGGCAGATAAACAAAAACGCCGGCAATGTGCCGGCGTTTTTGTTGTGCGAAGCAAGAGGGCGTATGCGGATAATCAGTCCAGCAGGGTTGCCCAGCttt                                                                         >  8:57814/1‑148 (MQ=255)
                                                               tGGCAACTGCATGCTCGACAATTGCAGGAACGCTATTCAATCGGCTGA‑TGGCAGATAAACAAAAACGCCGGCAATGTGCCGGCGTTTTTGTTGTGCGAAGCAAGAGGGCGTATGCGGATAATCAGTCCAGCAGGGTTGCCCAGCTTTc                                                                        >  6:82180/1‑148 (MQ=255)
                                                                        cATGCTCGACAATTGCAGGAACGCTATTCAATCGGCTGA‑TGGCAGATAAACAAAAACGCCGGCAATGTGCCGGCGTTTTTGTTGTGCGAAGCAAGAGGGCGTATGCGGATAATCAGTCCAGCAGGGTTGCCCAGCTTTCAACCACGt                                                                <  6:181057/147‑1 (MQ=255)
                                                                            cTCGACAATTGCAGGAACGCTATTCAATCGGCTGA‑TGGCAGATAAACAAAAACGCCGGCAATGTGCCGGCGTTTTTGTTGTGCGAAGCAAGAGGGCGTATGCGGATAATCAGTCCAGCAgg                                                                                      <  7:107216/121‑1 (MQ=255)
                                                                            cTCGACAATTGCAGGAACGCTATTCAATCGGCTGA‑TGGCAGATAAACAAAAACGCCGGCAATGTGCCGGCGTTTTTGTTGTGCGAAGCAAGAGGGCGTATGCGGATAATCAGTCCAGCAgg                                                                                      >  8:107216/1‑121 (MQ=255)
                                                                             tCGACAATTGCAGGAACGCTATTCAATCGGCTGA‑TGGCAGATAAACAAAAACGCCGGCAATGTGCCGGCGTTTTTGTTGTGCGAAGCAAGAGGGCGTATGCGGATAATCAGTCCAGCAGGGTTGCCCAGCTTTCACCCACGTCgccgcc                                                         >  8:128773/1‑149 (MQ=255)
                                                                             tCGACAATTGCAGGAACGCTATTCAATCGGCTGA‑TGGCAGATAAACAAAAACGCCGGCAATGTGCCGGCGTTTTTGTTGTGCGAAGCAAGAGGGCGTATGCGGATAATCAGTCCAGCAGGGTTGCCCAGCTTTCAACCACGTCgccgcc                                                         >  7:84254/1‑149 (MQ=255)
                                                                               gACAATTGCAGGAACGCTATTCAATCGGCTGA‑TGGCAGATAAACAAAAACGCCGGCAATGTGCCGGCGTTTTTGTTGTGCGAAGCAAGAGGGCGTATGCGGATAATCAGTCCAGCAGGGTTGCCCAGCTTTCAACCACGTCGCCGccc                                                        <  3:125482/148‑1 (MQ=255)
                                                                                 cAATTGCAGGAACGCTATTCAATCGGCTGA‑TGGCAGATAAACAAAAACGCCGGCAATGTGCCGGCGTTTTTGTTGTGCGAAGCAAGAGGGCGTATGCGGATAATCAGTCCAGCAGGGTTGCCCAGCTTTCAACCACGTCGCCGCCCCaa                                                     <  8:84254/149‑1 (MQ=255)
                                                                                  aaTTGCAGGAACGCTATTCAATCGGCTGA‑TGGCAGATAAACAAAAACGCCGGCAATGTGCCGGCGTTTTTGTTGTGCGAAGCAAGAGGGCGTATGCGGATAATCAGTCCAGCAGGGTTGCCCAGCTTTCAACCACGTCGCCGCACCAAg                                                    >  2:247316/1‑149 (MQ=255)
                                                                                       cAGGAACGCTATTCAATCGGCTGA‑TGGCAGATAAACAAAAACGCCGGCAATGTGCCGGCGTTTTTGTTGTGCGAAGCAAGAGGGCGTATGCGGATAATCAGTCCAGCAGGGTTGCCCAGCTTTCAACCACGTCGCCGCCCCAAGTGGCt                                               >  1:27165/1‑149 (MQ=255)
                                                                                        aGGAACGCTATTCAATCGGCTGA‑TGGCAGATAAACAAAAACGCCGGCAATGTGCCGGCGTTTTTGTTCTGCGAAGCAAGAGGGCGTATGCGGATAATCAGTCCAGCAGGGTTGCCCAGCTTTCAACCACGTCGCCGCCCCAAGTGGCt                                               >  1:184530/1‑148 (MQ=255)
                                                                                          gAACGCTATTCAATCGGCTGA‑TGGCAGATAAACAAAAACGCCGGCAATGTGCCGGCGTTTTTGTTGTGCGAAGCAAGAGGGCGTATGCGGATAATCAGTCCAGCAGGGTTGCCCAGCTTTCAACCACGTCGCCGCCCCAAGTGGCTTTc                                            >  7:101990/1‑149 (MQ=255)
                                                                                                tATTCAATCGGCTGA‑TGGCAGATAAACAAAAACGCCGGCAATGTGCCGGCGTTTTTGTTGTGCGAAGCAAGAGGGCGTATGCGGATAATCAGTCCAGCAGGGTTGCCCAGCTTTCAACCACGTCGCCGCCCCAAGTGGCTTTCCActct                                      >  6:65267/1‑149 (MQ=255)
                                                                                                  ttCAATCGGCTGA‑TGGCAGATAAACAAAAACGCCGGCAATGTGCCGGCGTTTTTGTTGTGCGAAGCAag                                                                                                                    <  1:85388/69‑1 (MQ=255)
                                                                                                  ttCAATCGGCTGA‑TGGCAGATAAACAAAAACGCCGGCAATGTGCCGGCGTTTTTGTTGTGCGAAGCAag                                                                                                                    >  2:85388/1‑69 (MQ=255)
                                                                                                     aaTCGGCTGA‑TGGCAGATAAACAAAAACGCCGGCAATGTGCCGGCGTTTTTGTTGTGCGAAGCAAGAGGGCGTATGCGGATAATCAGTCCAGCAGGGTTGCCCAGCTTTCa                                                                       <  4:95464/111‑1 (MQ=255)
                                                                                                     aaTCGGCTGA‑TGGCAGATAAACAAAAACGCCGGCAATGTGCCGGCGTTTTTGTTGTGCGAAGCAAGAGGGCGTATGCGGATAATCAGTCCAGCAGGGTTGCCCAGCTTTCAACCACGTCGCCGCCCCAAGTGGCTTTCCACTCTTTCAg                                 >  5:254072/1‑149 (MQ=255)
                                                                                                     aaTCGGCTGA‑TGGCAGATAAACAAAAACGCCGGCAATGTGCCGGCGTTTTTGTTGTGCGAAGCAAGAGGGCGTATGCGGATAATCAGTCCAGCAGGGTTGCCCAGCTTTCAACCACGTCGCCGCCCCAAGTGGCTTTCCACTCTTTCAg                                 <  7:57814/149‑1 (MQ=255)
                                                                                                      ataggCTGA‑TGGCAGATAAACAAAAACGCCGGAAATGTGACGGCGTTTTTGTTGTGCGAAGCAAGAGGGCGTATGCGGATAATCAGTCCAGCAGGGTTGCCCAGCTTTCAACCACGTCGCCGCCCCAAGTGGCTTTCCACTCTTTCAgg                                <  6:141914/146‑1 (MQ=255)
                                                                                                      aTCGGCTGA‑TGGCAGATAAACAAAAACGCCGGCAATGTGCCGGCGTTTTTGTTGTGCGAAGCAAGAGGGCGTATGCGGATAATCAGTCCAGCAGGGTTGCCCAGCTTTCAACCACGTCGCCGCCCCAAGTGGCTTTCCACTCTTTCAgg                                <  1:247316/149‑1 (MQ=255)
                                                                                                            ggA‑TGGCAGATAAACAACAACGCCGGCAATGTGCCGGCGTTTTTGTTGTGCGAAGCAAGAGGGCGTATGCGGATAAGCAGTCCAGCAGGGTTGCCCAGCTTTCAACCACGTCGCCGCCCCAAGTGGCTTTCCACTCTTTCAGGGTCTtg                          <  2:221597/148‑1 (MQ=255)
                                                                                                              a‑TGGCAGATAAACAAAAACGCCGGCAATGTGCCGGCGTTTTTGTTGTGCGAAGCAAGAGGGCGTATGCGGATAATCAGTCCAGCAGGGTTGCCCAGCTTTCAACCACGTCGCCGCCCCAAGTGGCTTTCCACTCTTTCAGGGTCTtgt                         <  6:254072/148‑1 (MQ=255)
                                                                                                              a‑TGGCAGATAAACAAAAACGCCGGCAATGTGCCGGCGTTTTTGTTGTGCGAAGCAAGAGGGCGTATGCGGATAATCAGTCCAGCAGGGTTGCCCAGCTTTCAACCACGTCGCCGCCCCAAGTGGCTTTCCACTCTTTCAGGGTCTtg                          <  2:187151/147‑1 (MQ=255)
                                                                                                                    aGATAAACAAAAACGCCGGCAATGTGCCGGCGTTTTTGTTGTGCGAAGCAAGAGGGCGTATGCGGATAATCAGTCCAGCAGGGTTGCCCAGCTTTCAACCACGTCGCCGCCCCAAGTGGCTTTCCACTCTTTCAGGGTCTTGTGGTTgc                   >  7:235835/1‑149 (MQ=255)
                                                                                                                    aGATAAACAAAAACGCCGGCAATGTGCCGGCGTTTTTGTTGTGCGAAGCAAGAGGGCGTATGCGGATAATCAGTCCAGCAGGGTTGCCCAGCTTTCAACCACGTCGCCGCCCCAAGTGGCTTTCCACTCTTTCAGGGTCTTGTGGTTgc                   <  4:132099/149‑1 (MQ=255)
                                                                                                                         aaCAAAAACGCCGGCACATTGCCGGCGTTTTTGTTGTGCGAAGCAAGAGGGCGTATGCGGATAATCAGTCCAGCAGGGTTGCCCAGCTTTCAACCACGGCGCCGCCCCAAGTGGCTTTCCACTCTTTCAGGGTCTTGTGGTTGCCGCCt              >  7:141562/1‑149 (MQ=255)
                                                                                                                           cAAAAACGCCGGCAATGTGCCGGCGTTTTTGTTGTGCGAAGCAAGAGGGCGTATGCGGATAATCAGTCCAGCAGGGTTGCCCAGCTTTCAACCACGTCGCCGCCCCAAGTGGCTTTCCACTCTTTCAGGGTCTTGTGGTTGCCGCCttt            <  8:101990/149‑1 (MQ=255)
                                                                                                                            aaaaaCGCCGGCAATGTGCCGGCGTTTTTGTTGTGCGAAGCAAGAGGGCGTATGCGGATAATCAGTCCAGCAGGGTTGCCCAGCTTTCAACCACGTCGCCGCCCCAAGTGGCTTTCCACTCTTTCAGGGTCTTGTGGTTGCCGCCTTTg           <  8:235835/149‑1 (MQ=255)
                                                                                                                             aaaaCGCCGGCACATTGCCGGCGTTTTTGTTGTGCGAAGCAAGAGGGCGTATGCGGATAATCAGTCCAGCAGGGTTGCCCAGCTTTCAACCACGTCGCCGCCCCAAGTGGCTTTCCACTCTTTCAgg                                >  2:253935/1‑127 (MQ=255)
                                                                                                                             aaaaCGCCGGCACATTGCCGGCGTTTTTGTTGTGCGAAGCAAGAGGGCGTATGCGGATAATCAGTCCAGCAGGGTTGCCCAGCTTTCAACCACGTCGCCGCCCCAAGTGGCTTTCCACTCTTTCAgg                                <  1:253935/127‑1 (MQ=255)
                                                                                                                              aaaCGCCGGCAATGTGCCGGCGTTTTTGTTGTGCGAAGCAAGAGGGCGTATGCGGATAATCAGTCCAGCAGGGTTGCCCAGCTTTCAACCACGTCGCCGCCCCAAGTGGCTTTCCTCTCTTTCACGGTCTTGTGGTTGCCGCCTTTGGt         >  6:65749/1‑149 (MQ=255)
                                                                                                                               aaCGCCGGCAATGTGCCGGCGTTTTTGTTGTGCGAAGCAAGAGGGCGTATGCGGATAATCAGTCCAGCAGGGTTGCCCAGCTTTCAACCACGTCGCCGCCCCAAGTGGCTTTCCACTCTTTCAGGGTCTTGTGGTTGCCGCCTTTGGtt        <  5:82180/149‑1 (MQ=255)
                                                                                                                                aCGCCGGCAATGTGCCGGCGTTTTTGTTGTGCGAAGCAAGAGGGCGTATGCGGATAATCAGTCCAGCAGGGTTGCCCAGCTTTCAACCACGTCGCCGCCCCAAGTGGCTTTCCACTCTTTCAGGGTCTTGTGGTTGCCGCCTTTGGttt       <  5:65267/149‑1 (MQ=255)
                                                                                                                                      gCAATGTGCCGGCGTTTTTGTTGTGCGAAGCAAGAGGGCGTATGCGGATAATCAGTCCAGCAGGGTTGCCCAGCTTTCAACCACGTCGCCGCCCCAAGTGGCTTTCCACTCTTTCAGGGTCTTGTGGTTGCTGCCTTTGGTTTCGATc  >  4:42923/1‑148 (MQ=255)
                                                                                                                                           |                                                                                                                                              
GAAAAGGCGCGCCAGCAAGCCTGGGAGGGCGCCGACGAGGCAGGCAGGCGCGTGTTGGAGGCCTGGCAACTGCATGCTCGACAATTGCAGGAACGCTATTCAATCGGCTGA‑TGGCAGATAAACAAAAACGCCGGCAATGTGCCGGCGTTTTTGTTGTGCGAAGCAAGAGGGCGTATGCGGATAATCAGTCCAGCAGGGTTGCCCAGCTTTCAACCACGTCGCCGCCCCAAGTGGCTTTCCACTCTTTCAGGGTCTTGTGGTTGCCGCCTTTGGTTTCGATC  >  NC_002947/1555252‑1555532

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑